• In enzymology, an asparagine-tRNA ligase (EC 6.1.1.22) is an enzyme that catalyzes the chemical reaction ATP + L-asparagine + tRNAAsn ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + L-asparaginyl-tRNAAsn The 3 substrates of this enzyme are ATP, L-asparagine, and tRNA(Asn), whereas its 3 products are AMP, diphosphate, and L-asparaginyl-tRNA(Asn). (wikipedia.org)
  • The systematic name of this enzyme class is L-asparagine:tRNAAsn ligase (AMP-forming). (wikipedia.org)
  • Analysis of the regions flanking mip identified three tightly linked open reading frames coding for predicted products with sequence similarity to asparagine tRNA ligase (AspS), rRNA methylase (SpoU), and thioredoxin (TrxA). (oregonstate.edu)
  • Glycine-tRNA ligase, alpha subunit [Interproscan]. (ntu.edu.sg)
  • Other names in common use include asparaginyl-tRNA synthetase, asparaginyl-transfer ribonucleate synthetase, asparaginyl transfer RNA synthetase, asparaginyl transfer ribonucleic acid synthetase, asparagyl-transfer RNA synthetase, and asparagine translase. (wikipedia.org)
  • 4560 valyl-tRNA synthetase CP001857 CDS Arcpr_0004 4868. (go.jp)
  • 55056) tyrosyl-tRNA synthetase CP001857 CDS Arcpr_0065 complement(55112. (go.jp)
  • This domain is found at the amino terminus of Arginyl tRNA synthetase, also called additional domain 1 (Add-1). (embl-heidelberg.de)
  • Crystal structure of arginyl-tRNA synthetase from Klebsiella pneumoniae subsp. (embl-heidelberg.de)
  • However, if aspargine synthase and asparginyl-tRNA synthetase (asnRS) are absent, then we can conclude that the aspartyl-tRNA synthetase is non-discriminatory. (lbl.gov)
  • It is biosynthesized from aspartic acid and ammonia by asparagine synthetase. (rcsb.org)
  • The absence of asparagine synthetase and the presence of a mutant tRNA for asparagine encoded among RNA-active enzymes suggest that P. carbinolicus may make asparaginyl-tRNA in a novel way. (umass.edu)
  • Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding [Interproscan]. (ntu.edu.sg)
  • Serine-tRNA synthetase, type1, N-terminal [Interproscan]. (ntu.edu.sg)
  • Aminoacyl-tRNA synthetase, class II (D/K/N) [Interproscan]. (ntu.edu.sg)
  • This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. (wikipedia.org)
  • This enzyme participates in alanine and aspartate metabolism and aminoacyl-trna biosynthesis. (wikipedia.org)
  • The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. (embl-heidelberg.de)
  • In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. (embl-heidelberg.de)
  • Serum metabolomics analysis showed that the aqueous extracts of PA could alleviate myrosuppression by regulating the aminoacyl-tRNA, valine, leucine and isoleucine biosynthesis mediated by 13 different metabolites such as valine, leucine, asparagine and hydroxyisohexic acid. (bvsalud.org)
  • However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. (embl-heidelberg.de)
  • Also, the presence of the tRNA-dependent amidotransferase gatABC is not sufficient to conclude that aspS2 is present because gatABC are also involved in tRNA-dependent synthesis of glutamine. (lbl.gov)
  • A modified nucleoside which is present in the first position of the anticodon of tRNA-tyrosine, tRNA-histidine, tRNA-asparagine and tRNA-aspartic acid of many organisms. (ucdenver.edu)
  • Here, we show that Nsp5 binds and cleaves human tRNA methyltransferase 1 (TRMT1), a host enzyme required for a prevalent post-transcriptional modification in tRNAs. (elifesciences.org)
  • This manuscript provides important insights into the degradation of a host tRNA modification enzyme TRMT1 by SARS-CoV-2 protease nsp5. (elifesciences.org)
  • 42520) hypothetical protein CP001857 tRNA Arcpr_R0002 complement(42639. (go.jp)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing Arg_tRNA_synt_N domain in the selected taxonomic class. (embl-heidelberg.de)
  • Human cells infected with SARS-CoV-2 exhibit a decrease in TRMT1 protein levels and TRMT1-catalyzed tRNA modifications, consistent with TRMT1 cleavage and inactivation by Nsp5. (elifesciences.org)
  • There are 26085 Arg_tRNA_synt_N domains in 26083 proteins in SMART's nrdb database. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing Arg_tRNA_synt_N domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with Arg_tRNA_synt_N domain is also avaliable . (embl-heidelberg.de)
  • Purification of individual SARS-CoV-2 proteins from human cells have identified a potential interaction between a catalytic-inactive version of Nsp5 with human tRNA methyltransferase 1 (TRMT1) ( Gordon et al , 2020b ). (elifesciences.org)
  • The TRMT1 cleavage fragments exhibit altered RNA binding activity and are unable to rescue tRNA modification in TRMT1-deficient human cells. (elifesciences.org)
  • These findings provide evidence that Nsp5-dependent cleavage of TRMT1 and perturbation of tRNA modification patterns contribute to the cellular pathogenesis of SARS-CoV-2 infection. (elifesciences.org)
  • This also indicates the presence of the tRNA-dependent pathway. (lbl.gov)
  • Amino acid levels are reduced and uncharged tRNA levels are increased in the islets of mice fed a HFD. (jci.org)
  • It is believed to play a role in the regulatory function of tRNA. (ucdenver.edu)
  • This enzyme participates in alanine and aspartate metabolism and aminoacyl-trna biosynthesis. (wikipedia.org)
  • In pathway III, aspartate is ligated to tRNA(Asn) and then amidated to Asn-tRNA(Asn). (lbl.gov)
  • Both secreted and also cell floor human EGFDs are generally at the mercy of substantial selleck chemicals llc adjustments, such as aspartate and also asparagine residue C3-hydroxylations catalyzed with the 2-oxoglutarate oxygenase aspartate/asparagine-β-hydroxylase (AspH). (micrornalibrary.com)
  • In the mitochondrial decoding system of echinoderms, including organisms like sea urchins and starfish, the AAA codon specifies the amino acid asparagine (Asn) instead of lysine (Lys). (u-tokyo.ac.jp)
  • The research group led by Assistant Professor Asuteka Nagao and Professor Tsutomu Suzuki of the Department of Chemistry and Biotechnology at the University of Tokyo's Graduate School of Engineering analyzed mitochondrial tRNA Lys isolated from the Mesocentrotus nudus sea urchin, and discovered a novel modified base, hydroxyl- N 6 -threonylcarbamoyladenosine (ht 6 A), in a part adjacent to the anticodon. (u-tokyo.ac.jp)
  • Sequencing of the mitochondrial genome (mtDNA) identified a transition variant in the tRNA asparagine gene (MT-TN) (m.5728T>C) at in 41% of mtDNA molecules in muscle tissue. (mcmaster.ca)
  • The mt tRNA gene was annotated using multi-software combined with a manual annotation approach. (biomedcentral.com)
  • The use of multi-software combined with a manual annotation approach can improve the accuracy of tRNA gene annotation. (biomedcentral.com)
  • 22. tRNA-derived fragments (tRFs) regulate post-transcriptional gene expression via AGO-dependent mechanism in IL-1β stimulated chondrocytes. (nih.gov)
  • These lead to the recruitment of a corresponding tRNA that ends transcription of the gene. (ubc.ca)
  • As each triplet codon is read (for example AGC), a transfer RNA (tRNA) molecule brings a specific amino acid to the ribosome, and this amino acid is then chemically joined to the previous amino acid by a peptide bond. (ubc.ca)
  • After the tRNA molecule delivers its amino acid to the translation complex, it floats away to be recharged with another amino acid. (ubc.ca)
  • 32. A novel class of small RNAs: tRNA-derived RNA fragments (tRFs). (nih.gov)
  • Overview: Asparagine biosynthesis in GapMind is based on MetaCyc pathways L-asparagine biosynthesis I ( link ), II ( link ), or III (tRNA-dependent) ( link ). (lbl.gov)
  • In this review, we highlight the essential role of AdT for the faithful translation of glutamine and/or asparagine codons, we describe important features of the crystal structures of several GatCAB as well as tRNA/AdT/aaRS complexes for the formation of Gln-tRNAAsn and Asn-tRNAAsn, we finally summarize discoveries of AdT inhibitors based on their analogy to glutamine, adesosine tripoliphosphate and 3'-end of tRNA. (psychiatryhealthjournal.com)
  • The discovery that certain mutated tRNAs could suppress these amber or stop codons was made thirty years ago [3]. (ubc.ca)
  • The mRNA is divided into codons, three-letter "words" made up of nucleotide bases, and each codon matches up with a particular transfer RNA (tRNA). (nih.gov)
  • The others, selenocysteine and pyrrolysine use tRNAs that are able to base pair with stop codons in the mRNA during translation. (pressbooks.pub)
  • Also there are no hits for some of the terms -- often, only gatB is annotated as a aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit. (lbl.gov)
  • Although the loss of genes is rare in Sarcoptiformes mite mitogenomes, two of the six previously reported oribatid mites (Acariforms: Sarcoptiformes) are reported to have lost parts of their tRNA genes. (biomedcentral.com)
  • To confirm whether the tRNA genes were indeed lost and whether the loss is universal, we re-annotated the available oribatid mite sequences and sequenced the mitogenome of Oribatula sakamorii . (biomedcentral.com)
  • The mitogenomes of O . sakamorii contained 37 genes, including 22 tRNA genes. (biomedcentral.com)
  • We identified all mt tRNA genes that were reported as "lost" in Steganacarus magnus and Paraleius leontonychus and revealed certain atypical tRNA annotation errors in oribatid mite sequences. (biomedcentral.com)
  • Rather, highly atypical secondary structure of the inferred mt tRNA genes made them unidentifiable using a single type of tRNA search program. (biomedcentral.com)
  • 28. Transfer RNA-derived fragments as potential exosome tRNA-derived fragment biomarkers for osteoporosis. (nih.gov)
  • 29. Plasma tRNA Fragments Derived from 5' Ends as Novel Diagnostic Biomarkers for Early-Stage Breast Cancer. (nih.gov)
  • 35. Antitumor Activities of tRNA-Derived Fragments and tRNA Halves from Non-pathogenic Escherichia coli Strains on Colorectal Cancer and Their Structure-Activity Relationship. (nih.gov)
  • 39. Expression profiles of tRNA-derived fragments and their potential roles in lung adenocarcinoma. (nih.gov)
  • This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. (wikipedia.org)
  • Thus, the efficiency regarding translation firing is actually regulated through the structure-switching biosensors 3′ nucleotide wording following end codon and also depends upon the actual levels associated with eRFs as well as suppressor/near-cognate tRNAs.Skin growth factor-like domains (EGFDs) get important capabilities throughout cell-cell signaling. (micrornalibrary.com)
  • These ARSs essentially ensure that the tRNA waiters pick up the right order. (ubc.ca)
  • GatDE are thought to form glutaminyl-tRNA only, so they are not described here (but TIGRFam suggests that gatD might replace gatB in some archaea). (lbl.gov)