Treatment cycloheximide. Medical search. Frequent questions

A study was conducted to determine the effects of pretreatment at varying concentrations of Cycloheximide (CHI) on senescence and postharvest performance in Nerine sarniensis . (scialert.net)
The present study was undertaken to investigate the effects of pretreatment with different concentrations of Cycloheximide (CHI) before transfer to Distilled Water (DW) or Sucrose (0.15 M) on senescence with the ultimate aim to gain an insight into the mechanism of senescence and to improve its postharvest performance. (scialert.net)
The harvested scapes were immediately brought to the laboratory, cut to a uniform size of 30 cm and pulse treated for 1 h separately with varying concentrations of cycloheximide (0.01, 0.05, 0.1 and 0.5 mM). (scialert.net)
The change in distribution of gene 5 mRNA in polyribosome gradients in response to treatment with low concentrations of cycloheximide suggested that gene 5 is a poor translation initiation template compared with gene 6 mRNA. (microbiologyresearch.org)

In consequence, protein turnover of WT-TRPM4 and TRPM4 variants overexpressed in HEK293 cells was analyzed using cycloheximide, an inhibitor of protein biosynthesis. (frontiersin.org)
Cycloheximide, an inhibitor of protein synthesis, did not alter Cd-induced cytotoxicity indicating that J774A.1 cell death by Cd is independent of de novo protein synthesis including p53. (cdc.gov)

We isolated P. orbiculare on a peptone-glucose-yeast extract medium containing chloramphenicol and cycloheximide, and overlaid with olive oil. (who.int)

Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking eukaryotic translational elongation. (wikipedia.org)
The translational elongation freezing properties of cycloheximide are also used for ribosome profiling / translational profiling. (wikipedia.org)
Pretreatment with transcriptional (actinomycin D), translational (cycloheximide) or HSF1 (KNK437) inhibitors prevented the ITC-induced accumulation of HO-1, HSP70 and HSP30. (uwaterloo.ca)

Pretreatment of scapes with 0.05 or 0.01 CHI for 1 h can be used as an effective treatment to improve the post harvest longevity in this flower system. (scialert.net)
Cycloheximide or Chk2 inhibitor pretreatment abrogated not only activation of Chk2 but also G 2 /M arrest and apoptosis mediated by DIM. (aspetjournals.org)

The following represents susceptibility data for a few commonly targeted fungi: Candida albicans: 12.5 μg/ml Mycosphaerella graminicola: 47.2 μg/ml - 85.4 μg/ml Saccharomyces cerevisiae: 0.05 μg/ml - 1.6 μg/ml Neoscytalidium dimidiatum is an Athlete's foot like infection resistant to most antifungals but is rather sensitive to cycloheximide, so, it should be cultured in a medium free of cycloheximide. (wikipedia.org)
0.05) increase in IRES use under LA treatment. (nih.gov)

All the samples were processed in Campylobacter Enrichment Hi VegTM Broth Base (HiMedia, Mumbai) with addition of polymyxin B sulphate, rifampicin, trimethoprim and cycloheximide ( Campylobacter selective IV, HiMedia, Mumbai) and incubated at 42-43°C for 24 h. (scialert.net)

Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). (wikipedia.org)
Mitochondrial protein synthesis is resistant to inhibition by cycloheximide. (wikipedia.org)
Furthermore, studies using cycloheximide indicate that these GH-induced changes in mRNA expression do not require protein synthesis and hence are primary, not secondary events. (aacc.org)
On the contrary, treatment with the protein synthesis inhibitors cycloheximide, anisomycin, or puromycin as well as prolonged exposure to the RNA synthesis inhibitor actinomycin D mimicked the biochemical modulating effects of 6AN on cisplatin action. (aspetjournals.org)
Cycloheximide treatment also causes cytokeratin filaments to disappear, indicating that protein synthesis is needed for normal filament maintenance. (rupress.org)
Protein stability was determined following treatment with protein synthesis inhibitor cycloheximide and proteasome inhibitor MG132. (thno.org)

Treatment of CRC cells with MS-444 resulted in growth inhibition and increased apoptotic gene expression, while similar treatment doses in non-transformed intestinal cells had no appreciable effects. (oncotarget.com)

EGTA treatment, by disassembling desmosomes, causes the cytokeratin filaments to disappear and the 24B42 protein to pass into a soluble form in this cell line, as ascertained by 100,000 g fractionation and immunoenzymatic assay. (rupress.org)

Western blot analysis determined that HSPB6 was present constitutively in A6 cells, induced by MG132 and slightly by cadmium chloride treatment but not by heat shock. (uwaterloo.ca)

Cycloheximide has also been used to make isolation of bacteria from environmental samples easier. (wikipedia.org)
Experiments comprised 48-h incubations in 3 treatment variants: bacteria with protozoa, bacteria with protozoa added after some time and bacteria without protozoa. (ufz.de)
Illumina 16S rDNA amplicon sequence analysis of culturable L. terrestris -associated bacteria showed that antibiotic treatment influenced community composition revealing putative sensitive (Comomonas, Kosakonia and Sphingobacterium) and insensitive (Aeromonas, Pseudochrobactrum) taxa. (nerc.ac.uk)

A transient treatment of growing wild type maize shoots with exogenous CK also induced JA pathway gene expression, although this effect was blocked by co-treatment with cycloheximide. (usda.gov)

Cycloheximide has been used to isolate dermatophytes and inhibit the growth of fungi in brewing test media. (wikipedia.org)

Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life. (wikipedia.org)
and 2) simultaneous treatment of cells with 6AN and cycloheximide prevented the increase in GRP78 but not the sensitizing effect of 6AN. (aspetjournals.org)
Immunoblot analysis revealed that treatment of cells with each of the ITCs induced the accumulation of HO-1 and HSP70 while HSP30 levels were only enhanced in cells treated with BITC at 30 °C. In contrast, HSPB6 levels were not affected by ITCs. (uwaterloo.ca)
However, treatment of cells with BITC or PEITC at 30 °C greatly enhanced microtubule fragmentation. (uwaterloo.ca)
The final chapter of this thesis investigated characteristics of the Xenopus small heat shock protein HSPB6, a HSP whose levels were not affected by ITC treatment of A6 cells. (uwaterloo.ca)
HeLa cells expressing Myc-GnT1IP-L (Myc-tagged GlcNAcT-1 inhibitory protein, long transcript, membrane-anchored) (red) and GlcNAcT-1-HA (green) were treated with cycloheximide and brefeldin A to cause retrograde transport of Golgi enzymes into the ER. (cellimagelibrary.org)
These factors can be induced in G(0) cells by UV irradiation and inhibited in G(1) cells by cycloheximide treatment. (rupress.org)
DIM treatment inhibited the growth of SKOV-3, TOV-21G, and OVCAR-3 ovarian cancer cells in both a dose- and time-dependent manner with effective concentrations ranging from 40 to 100 μM. (aspetjournals.org)

Dogs sharing a household with another dog, dogs that had not received antibiotic treatment in the previous months and the age of the dog were significant indicators of Campylobacter carriage. (scialert.net)

It can also be combined with systemic oral antifungals providing more limited treatment time. (hindawi.com)
The downside of the antifungals are that they require blood testing to monitor the liver because they are systemic and also that they require long treatment courses (approximately 6 months for toenails and 4 months for fingernails). (hindawi.com)

Treatment with Cd at 20 and 50 microM induced phosphorylation of extracellular signal-regulated kinase (ERK), but did not alter p53 mRNA expression or the activation of p38 MAPK and c-Jun N-terminal MAPK. (cdc.gov)

To characterize endogenous substances and activities from the Golgi organic, protein in transit were 99% cleared from rat hepatocytes through the use of cycloheximide (CHX) treatment. (bioinf.org)

The fact that cycloheximide delays tepal senescence demonstrates the synthesis of particular proteins probably enzymes, responsible for degradation of cellular constituents, executes the cell death programme in flower tepals. (scialert.net)
Both proteins had punctate costaining around the nucleus, typical of ERGIC localization, in addition to reticular costaining of the ER after BFA treatment. (cellimagelibrary.org)

Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. (wikipedia.org)

Dr. Gronowski's work has examined the early events that occur after in vivo growth hormone (GH) treatment. (aacc.org)
These studies have demonstrated that in vivo GH treatment results in the activation of both the JAK/STAT and MAP kinase pathways in hepatic tissue. (aacc.org)
Recovery of enzymatic activity in vivo, which occurred within 24 h of low-dose MDMA treatment, appeared not to involve synthesis of new enzyme protein, because the return of enzymatic activity was not prevented by prior cycloheximide. (erowid.org)
In vivo treatment of MS-444 inhibited HuR cytoplasmic localization and decreased COX-2 expression in tumors. (oncotarget.com)

Upon addition of cycloheximide, WT-TRPM4 decayed with a half-life of ~20 h, while loss-of-expression variants showed a ~30% increase in degradation rate, with a half-life close to 12 h. (frontiersin.org)

13 Because onychomycosis in diabetic patients can lead to many complications,most insurance companies cover treatment in documented cases. (diabetesjournals.org)

Since a great deal of the molecular and cellular research with Xenopus is applicable to humans, an increase in our knowledge of the effects of ITCs is of importance given their potential role as therapeutic compounds in the treatment of cancer, neurodegenerative diseases and bacterial and viral infections. (uwaterloo.ca)

Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. (wikipedia.org)
US Centers for Disease Control and Prevention (CDC) recommendations are stressed, and promising experimental treatments are noted. (medscape.com)

Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. (wikipedia.org)
In addition, these recommendations propose a national strategy for reducing the morbidity of chlamydial infections by detection and treatment and through the prevention of transmission to uninfected persons. (cdc.gov)

Classical treatment options include mechanical and chemical debridement, topical antifungal lacquers, systemic antifungal drugs, and finally various combinations of the above. (hindawi.com)
A combination of topical and systemic treatment increases the cure rate. (medscape.com)

and (2) differential filtration on non-selective medium incubated at 37°C. For both treatments, 0.5 g faeces was emulsified in 0.1% (wt/vol) peptone water. (bmj.com)
cycloheximide 0.5 g/L were added to the Also, Sharquie, Al-Rubyae and Al-Rawi medium. (who.int)

Thus, this central regulator could serve as a potential therapeutic target for treatment of HCC. (nature.com)

MDMA-inactivated cortical tryptophan hydroxylase derived from rats killed later than 3 days after drug treatment could not be significantly reactivated under the conditions described above, indicating the development of irreversible enzymatic damage. (erowid.org)

Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation. (wikipedia.org)

All subjects were well satisfied with the treatments, there were no noticeable side effects, and no significant differences were found treating men versus women. (hindawi.com)

Least significant difference (LSD) was used to determine variation among treatment groups. (druglib.com)

But, all these treatments fail to provide a promising functional outcome or decrease mortality. (karger.com)
The discussion focuses on diagnosis, treatment, and clinical outcome. (medscape.com)

Because cycloheximide rapidly breaks down in a basic environment, decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soapy water or aqueous sodium bicarbonate. (wikipedia.org)

5 Hunan Provincial Gynecological Cancer Diagnosis and Treatment Engineering Research Center, Changsha, China. (aacrjournals.org)
In accordance with the 2008 European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) criteria, the patient was classified as having probable IFI [ 23 ]. (biomedcentral.com)

Additionally, long lasting treatment means high treatment costs for both the patient and health insurers. (hindawi.com)

Cycloheximide is used as a plant growth regulator to stimulate ethylene production. (wikipedia.org)
Treatment of wild type seedlings with exogenous JA reduces maize leaf size and growth rate, while JA deficient maize mutants have increased leaf size and growth rate. (usda.gov)

Treatment duration was approximately 15 minutes per session and patients were observed over a 3-month time period. (hindawi.com)
There is a short-of-effective medical treatment for secondary inflammation and reducing brain edema in ICH patients. (karger.com)

SMAL activity was also destroyed by treatment with 5% TCA, 0.4 M HCl or 60% acetonitrile, but resistant to 6 M urea or dialysis against pH 2 buffer for 24 hours. (etsu.edu)

Attempts are made to include the most recent and relevant treatment options, emphasizing the use of pharmaceuticals and their impact. (medscape.com)

Finally, high recurrence rates have been described, 22% three years after completion of treatment and higher recurrence rates at five years follow-up [ 8 - 10 ]. (hindawi.com)

Immunocytochemical studies determined that ITC treatment also resulted in F-actin disorganization and membrane ruffling. (uwaterloo.ca)

6 In both treatments, plates were incubated in microaerophilic conditions (5% (vol/vol) O 2 ) for two to five days. (bmj.com)

Anatomy9

Organisms2

Chemicals and Drugs35

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes16

Information Science2

Concentrations of cycloheximide4

Inhibitor of protein2

Chloramphenicol1

Translational3

Pretreatment2

0.052

Trimethoprim1

Protein synthesis6

Inhibition1

Filaments1

MG1321

Bacteria3

Gene expression1

Dermatophytes1

Cells8

Antibiotic treatment1

Antifungals2

MRNA1

Golgi1

Proteins2

Reproductive1

Vivo4

Variants1

Onychomycosis1

Bacterial1

Experimental2

Addition2

Systemic2

Medium2

Therapeutic1

Significantly1

Translation1

Effects1

Significant1

Outcome2

Breaks1

Cancer2

Health1

Growth2

Patients2

Activity1

Options1

Years1

Studies1

Conditions1