Transplantation of mscs. Medical search. Frequent questions

The patients will receive autologous MSC, although substantially expanded in vitro prior to transplantation, thereby avoiding potential risk of HLA immunization, transfer of viral infections and other diseases. (uu.se)
Although liver failure can be treated via hepatocyte transplantation, it also faces multiple problems comprising the shortage of high-quality hepatocytes sources, rejection of allogeneic transplants, difficulty to expand, and losing hepatic characteristics in vitro [ 7 , 8 ]. (springer.com)
Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
Different groups have established several protocols to induce the differentiation of MSCs into HLCs in vitro . (springer.com)
The primary objective of this study was to verify whether a natural antioxidant, lycopene, could protect MSCs from ischemia-induced apoptosis in vitro. (nih.gov)
With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. (nature.com)
Nevertheless, MSCs can lose their differentiation potential after expansion in vitro and transplantation into the site of injury 7 . (nature.com)
In order to cope with this task, in vitro systems are required which allow studies regarding cell-material interactions to unfold the impact of material surface properties on MSCs. (nature.com)
The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
similarly, L-MSCs were significantly more resistant than BM-MSCs to anchorage independent culture (4 h) in vitro. (nih.gov)

Life-threatening therapy-resistant grade III and IV graft vs. host disease (GvHD) of the gut and liver was dramatically suppressed in patients after MSC infusion. (uu.se)
The only curative treatment for acute liver failure is liver transplantation, but there are many restrictions on the application of liver transplantation. (springer.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
The only curative treatment for ALF is liver transplantation, but there are many restrictions on the application of liver transplantation because of financial considerations, a shortage of donor livers, and immunosuppression-related complications [ 3 ]. (springer.com)
Hepatologist, Associate Professor of Medicine - Dr. Neil Mehta is a gastroenterologist and specialist in liver transplantation. (ilts.org)
His other research interests include outcomes for patients who undergo liver transplantation and cardiac iron overload in patients with chronic liver disease. (ilts.org)
Dr. Rammohan has also completed a two-year postdoctoral fellowship in Liver Transplantation. (ilts.org)
Parissa Tabrizian, MD, MSc, FACS is a board-certified surgeon, associate professor of surgery who specializes in hepatobiliary and liver transplantation at the Recanati/Miller Transplantation Institute. (ilts.org)
Her research interests include hepatocellular carcinoma, cholangiocarcinoma, and liver transplantation. (ilts.org)
Ye is a Professor of Autoimmune liver diseases and Translational Hepatology at the Institute of Immunology and Immunotherapy, University of Birmingham and Consultant Hepatologist at the Queen Elizabeth Hospital where he specialises in the management of autoimmune liver diseases, immune mediated liver injury and liver transplantation. (birmingham.ac.uk)
He underwent general medical training in Scotland, Wales and Bournemouth followed by specialisation in Gastroenterology, Hepatology and Liver Transplantation at the West Midlands Deanery. (birmingham.ac.uk)
During his PhD he dissected the recruitment mechanism of regulatory T cells and developed isolation of GMP-grade regulatory T cells to apply as new therapy in autoimmune liver diseases and liver transplantation. (birmingham.ac.uk)
Conditioned medium (CM) derived from AF-MSCs or HPL cells was then delivered intrahepatically in order to determine whether the engraftment of the cells or their secreted molecules are the most important agents for liver repair. (bmj.com)
In addition, HPL-CM was found to be more efficient than CM derived from AF-MSCs in treatment of the liver. (bmj.com)
Conclusions Human spindle-shaped AF-MSCs or HPL cells might be valuable tools to induce liver repair and support liver function by cell transplantation. (bmj.com)
Furthermore, microarray profiling identified 226 genes with differential expression in the liver of animals treated with MSC, with cellular signaling, and actin cytoskeleton regulation as key upregulated processes. (frontiersin.org)
The TTS-ILTS Paired Transplant Centers Program is a collaboration between The Transplantation Society (TTS) and the International Liver Transplantation Society (ILTS) supporting new liver transplant programs in emerging countries. (tts.org)
Post-liver transplantation (LT) recurrent or de novo hep atic steatosis is a common complication in recipients, irrespective of tran splantation indication. (wjgnet.com)
Liver transplant recipients are at high risk of developing insulin resistance, new-onset diabetes, and post-transplantation metabolic syndrome that is highly associated with immunosuppressive treatment. (wjgnet.com)
Tragically, in Europe, NAFLD is now the leading cause of liver transplantation. (medscape.com)
Treatment is surgical, with portojejunostomy (Kasai procedure) or liver transplantation. (msdmanuals.com)
Many patients are not candidates given the advanced stage of their cancer at diagnosis or their degree of liver disease and, ideally, could be cured by liver transplantation . (medscape.com)

In the 1980's, Arnold Caplan and his colleagues published an isolation method of fibroblast-like stromal cells from bone marrow and first identified them as mesenchymal stem cells (MSCs) because of their multilineage differentiation potential ( Caplan, 1991 ). (frontiersin.org)
In previous studies, a distinct population termed human spindle-shaped MSCs were isolated and expanded from second trimester amniotic fluid (AF-MSCs) and characterised based on their phenotype, pluripotency and differentiation potential. (bmj.com)
In this study, we characterized L-MSC phenotype, clonogenicity, and differentiation potential, and compared L-MSCs to BM-MSCs in vivo survival, retention, paracrine gene expression, and repair or elastase injury after transplantation. (nih.gov)

We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
How L-MSCs compare with bone marrow-derived MSCs (BM-MSCs) is also unclear. (nih.gov)

New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. (nature.com)
After bone fractures mesenchymal stromal cells (MSCs) take on a key role in the in vivo bone repair process 1 . (nature.com)
While multipotent mesenchymal stromal cells have been recently isolated from adult lung (L-MSCs), there is very limited data on their biological properties and therapeutic potential in vivo. (nih.gov)
Interestingly, MaR1 also regulated the actions of primary isolated human lung mesenchymal stem (stromal) cells (MSCs). (cdc.gov)

The transplantation of both autogenous and allogeneic MSCs into a defect of the growth plate appears as an effective method of surgical treatment of physeal cartilage injury. (muni.cz)

Human integrin α10β1-selected adipose tissue-derived MSCs (integrin α10β1-MSCs) have shown immunomodulatory and regenerative effects in various disease models. (lu.se)
MSCs can be isolated from different locations, such as bone marrow, umbilical cord, placenta, adipose and dental tissues. (bvsalud.org)

Potential systems of MSC-mediated safety from ischemic damage will be the differentiation of MSCs into cardiomyocytes, the excitement of cardiac stem cell proliferation, and/or cells that enhance vascularization [3]. (mycareerpeer.com)
The differentiation, proliferation, prognosis, and safety of MSCs for in vivo therapy remain controversial. (molvis.org)
The current finding suggested that MSC administration promoted proliferation and activation of CD163 + or CD206 + M2 Mφs in parallel with reduction of proinflammatory cytokines and increase in anti-inflammatory cytokines. (frontiersin.org)
However, cultures without the presence of MSCs showed higher NK proliferation, expression of activation markers (CD16 and NKG2D) and related cytotoxic activity. (springer.com)
Furthermore, we found that OMSCs are distinct from long bone MSCs with respect to regulating T-lymphocyte survival and proliferation. (ca.gov)

Nevertheless, the reduced retention price of MSCs after transplantation and their low propensity to differentiate right into a cardiac phenotype [4] make it improbable that cell alternative is the major mechanism of great benefit. (mycareerpeer.com)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
Bone marrow mesenchymal stem cells (MSCs) have been identified to have the potential to differentiate into multiple types of cells. (nih.gov)
MSCs have the ability to differentiate into adipocytes and chondrocytes forming fat tissue and cartilage as well as into bone cells - called osteocytes. (nature.com)
EMSC were generated by inducing hESC to differentiate into MSC using the method we reported previously. (nih.gov)

Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
In conclusion, MSCs in musculoskeletal system, mediated by the interaction between MSC paracrine and Mφs, have therapeutic potential in musculoskeletal diseases. (frontiersin.org)
Methods AF-MSCs, hepatic progenitor-like (HPL) cells and hepatocyte-like (HL) cells derived from AF-MSCs were transplanted into CCl 4 -injured NOD/SCID mice with the AHF phenotype in order to evaluate their therapeutic potential. (bmj.com)
This study further evaluated the therapeutic potential of UCM-MSCs in a mouse Parkinson's disease (PD) model. (cryo-cell.com)

Promising preliminary results are also emanating from an ongoing open randomized clinical study conducted in collaboration with Prof Katarina LeBlanc, KI, Stockholm, in which the efficacy of islet transplantation with or without the support of autologous MSC is investigated. (uu.se)
Overall, these findings greatly enhance the utilization of islets for islet transplantation, and also provide valuable insight for future work in reaggregating islet cells with MSCs. (ku.edu)

MSC have marked immunosuppressive capacity in vivo also in humans. (uu.se)
The use of donor derived MSCs for triggering the bone regeneration in vivo is a promising tool for assisting the healing process. (nature.com)
Interaction of MSCs with bone morphogenic proteins (BMPs) in an alginate scaffold mimicking the extracellular matrix (ECM) in vivo enhances their differentiation into bone cells 9 . (nature.com)
In vivo experiments confirmed mitochondrial abundance and mitochondrial oxygen consumption rates were elevated in host tissues following MSC treatment. (frontiersin.org)
Further, L-MSCs with low ICAM-1 showed poorer lung retention and higher phagocytosis in vivo. (nih.gov)
We developed techniques to isolate mouse OMSCs derived from mandibles and verified their MSC characteristics by single colony formation, multi-lineage differentiation, and in vivo tissue regeneration. (ca.gov)
UMSC transplantation with a fibrin plug improves the healing of injured corneas and regeneration of transparent corneas, as determined with in vivo HRT II confocal microscopy. (molvis.org)

Additionally, the project is committed to addressing issues of inequality and discrimination in the lung transplant process, focusing on improving access to transplantation for marginalized groups and simplifying the complex referral processes. (lu.se)
Globally, only a fraction of all patients have access to transplantation, and, even in the developed world, organ shortage remains a major limiting factor. (medscape.com)

The hypothesis tested in these projects is that the development of TID may be halted at diagnosis by the immunomodulatory properties of mesenchymal stem cells (MSC). (uu.se)
The bone marrow contains not only hematopoietic cells, but also mesenchymal stem cells (MSC). (uu.se)
Introduction The advantage of transplantation of bone tissue marrow-derived mesenchymal stem cells (MSCs) after cardiac infarction continues to be evaluated in lots of preclinical and scientific research [1,2]. (mycareerpeer.com)
It had been proven that MSC-conditioned moderate can promote vascularization, improve the activation of body-owned stem cells [3,5], boost cell success [6], and improve contractility or SGX-523 price mobile redesigning. (mycareerpeer.com)
MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
Hematopoietic stem cell transplantation (HSCT) is a broad term that covers the transplantation of blood progenitor/stem cells from any source. (drugdiscoverynews.com)
Their ongoing Phase 2 study is evaluating whether infusion of mesenchymal stem cells (MSCs) can treat steroid-resistant acute graft-versus-host disease (GVHD) or poor graft function after HSCT. (drugdiscoverynews.com)
One trial is testing whether MSCs with or without peripheral blood stem cells could treat poor graft function and delayed platelet engraftment. (drugdiscoverynews.com)
In another trial, this group is evaluating whether peripheral blood stem cells combined with MSCs can treat poor graft function. (drugdiscoverynews.com)
Human orofacial bone-derived mesenchymal stem cells (OMSCs) showed distinct differentiation traits from mesenchymal stem cells (MSCs) derived from long bones, mouse OMSCs have not been isolated due to technical difficulties, which in turn precludes using mouse models to study orofacial diseases. (ca.gov)
Next, the effect of creating hybrid islets/spheroids using mixtures of cells was tested by combining cells from different human donors, or by combining mesenchymal stem cells (MSCs) and islet cells. (ku.edu)
Mesenchymal stem cells (MSCs) have been studied for their potential benefits in treating acute respiratory distress syndrome (ARDS) and have reported mild effects when trialed within human clinical trials. (lu.se)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
The project recognizes the limitations of EVLP in treating all damaged lungs and therefore aims to develop new therapies, particularly based on mesenchymal stem cells (MSC). (lu.se)

Transplantation And Cellular Therapy 2023, 29: s387-s388. (yale.edu)

However, the rejection reaction often occurs in the transplantation of MSCs and could induce cell death. (nih.gov)

In December 1967 Christiaan Barnard performed the first human cardiac transplantation in Cape Town, South Africa. (bcmj.org)
Systemic disease processes are a contraindication to cardiac transplantation. (medscape.com)

During such period, Mφs also induced MSCs into a motile and active phenotype via the influence of proinflammatory cytokines. (frontiersin.org)
Col5a1 ∆st/∆st mice display a cloudy cornea phenotype that is ameliorated following intrastromal transplantation of UMSCs. (molvis.org)

Since MSCs secrete a range of development and cytokines elements, recent research offers centered on the relevance of paracrine signaling in MSC-mediated cardioprotection [5]. (mycareerpeer.com)
This review focused on the elucidation of paracrine crosstalk between MSCs and Mφs during musculoskeletal diseases and injury. (frontiersin.org)
Such crosstalk between Mφs and MSCs further strengthens the effect of paracrine mediators from MSCs to regulate Mφs phenotypic alteration. (frontiersin.org)

No adverse side effects of MSC treatment have yet been observed in these or other clinical studies. (uu.se)
The detailed analysis of circulating lymphocytes and their antigen-specific cellular responses will be instrumental in the understanding of the mechanisms behind the autoimmune progression towards TID and to monitor the effect of the transplanted MSC in clinical trials. (uu.se)
Although there is a great deal of preclinical and clinical research about MSCs in the treatment of ALF, the exact mechanism underlying the therapeutic role of MSCs remains unclear [ 6 ]. (springer.com)
Future mechanistic and therapeutic investigations are warranted to ascertain the clinical usefulness of these lipids in regulating lung repair processes relevant to lung transplantation. (cdc.gov)
In 2005, he travelled to the University of Maryland Medical Center in Baltimore to complete a cardiac surgery clinical fellowship focused on thoracic transplantation/mechanical circulatory support and minimal access cardiac surgery. (uwo.ca)
This was hypothesized to reflect a clinical picture of treatment with MSCs in. (lu.se)
The WCMM clinical researcher Sandra Lindstedt has currently received EU funding for the project "The bridge - lungs for life", which aims to address the critical shortage of viable lungs for transplantation. (lu.se)

In experimental animal models, MSC have been shown to improve the outcome of renal, neural and lung injury by promoting a shift from pro-inflammatory to anti-inflammatory cytokines at the site of injury. (uu.se)
This study demonstrates that tissue-specific L-MSCs possess mechanisms that enhance their lung retention after intravenous transplantation, and produce substantial healing of elastase injury comparable to BM-MSCs. (nih.gov)
During lung transplantation, wound healing must occur, yet unchecked responses can result in negative outcomes including bronchiolitis obliterans. (cdc.gov)
Our results identify DHA and MaR1 as regulators of reparative processes after lung inflammation and wounding, including effects on two pivotal cellular players-bronchial epithelial cells and MSCs. (cdc.gov)
MSCs have been investigated in preclinical models with efficacy when administered at the time of lung injury. (lu.se)
Lung transplantation is the only treatment for patients with severe lung disease, but the organs are not enough, resulting in patients dying while waiting for organs. (lu.se)
Overall, " The bridge - lungs for life " seeks to revolutionize lung transplantation in Scandinavia by combining centralized EVLP facilities, innovative therapies, and efforts to promote equality in access to transplant services. (lu.se)

The University of the West of England (UWE) and NHSBT MSc in Applied Transfusion and Transplantation Science is accepting applications to start in September 2022. (blood.co.uk)

In mice treated with daily low doses of the beta-cell toxin streptozotocin, MSC obtained from human bone marrow were found to home to and promote repair of pancreatic islets, thereby ameliorating the level of diabetes. (uu.se)

MSC-mediated cardioprotection from ischemia/reperfusion (I/R) injury has been demonstrated through preconditioning of the cardiac tissue [17]. (mycareerpeer.com)
MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)

We hypothesized that integrin α10β1 selected-MSCs can be used to treat a sepsis-induced ARDS in a porcine model when administering cells after established injury rather than simultaneously. (lu.se)
6 cells/kg integrin α10β1-selected MSCs and both groups were followed for 12 h. (lu.se)
This provides new opportunities to investigate integrin α10β1-selected MSCs as a treatment for a disease which does not yet have any definitive therapeutic options. (lu.se)

MSC also protected from diabetes in the non-obese diabetic (NOD) mouse model by inducing regulatory T-cells in pancreatic lymph nodes. (uu.se)

Further investigation is needed to understand the mechanisms of NK-MSC interaction and its implications. (springer.com)

Researchers from several academic institutions in China-headed by Qifa Liu, of the Nanfang Hospital of Southern Medical University-are also studying MSCs as a therapy for poor graft function. (drugdiscoverynews.com)
Sirolimus, also known as rapamycin (SRL, Rapamune®), was approved in 1999 by the US Food and Drug Administration to prevent graft rejection in renal transplantation. (medscape.com)

Our findings of xenotransplantation have collectively suggested the potential utility of UCM-MSCs in developing viable therapeutic strategies for PD. (cryo-cell.com)
these spheroids were highly viable even long-term in culture, but secreted less insulin irrespective of the proportion of MSCs and islet cells in the spheroids, when compared to the spheroids containing only islet cells. (ku.edu)

Posteroanterior and lateral chest radiographs: Used to screen for other thoracic pathologies that may preclude transplantation. (medscape.com)

MSC constitute only 0.001-0.01% of total nucleated cells, but have unique immunosuppressive capacities. (uu.se)
MSC-mediated inhibition of T-cells is also associated with a reduction in inflammatory cytokine production. (uu.se)
However, a postconditioning benefit of MSCs was also demonstrated when cells were injected 2?h after ischemic injury [21]. (mycareerpeer.com)
This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells' differentiation for the development of new bone regenerating strategies. (nature.com)
Therefore, it is important to understand how MSC differentiation into bone cells is controlled and how this process can be guided or improved by externally added factors. (nature.com)
NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
For spheroid formation, MSC were split and seeded on adhesion substrates by using handing drop method at a density of 2.5×104 cells/drop as we recently reported 15, and the MSC in the handing drop formed spheroids within 2-day culture. (nih.gov)
Either MSC spheroids or cells dissociated from MSC in monolayer were harvested for transplantation. (nih.gov)
MSC mediated bone formation involves in both donor and recipient cells, but only recipient cells contribute to marrow element formation. (ca.gov)
Experimental mice, i.e., wild-type, Col5a1 f/f and Kera-Cre/Col5a1 f/f ( Col5a1 ∆st/∆st , collagen V null in the corneal stroma) mice in a C57BL/6J genetic background, were subjected to a lamellar keratectomy, and treated with or without UMSC (10 4 cells/cornea) transplantation via an intrastromal injection or a fibrin plug. (molvis.org)

Heart transplantation is the replacement of a failing heart with a heart from a suitable donor. (medscape.com)
Fecal microbiota transplantation (FMT) involves the delivery of an entire microbial community from a healthy donor to a recipient with the intention of ameliorating or curing a specific disease. (aap.org)

These results highlight the potential of MSCs to modulate mitochondria in both inherited and pathological disease states. (frontiersin.org)
these proteins were found to modulate endothelial adherence, directional migration, and migration across Matrigel in L-MSCs. (nih.gov)

We intend to use autologous MSC for treatment of recently diagnosed patients with TID. (uu.se)
Organ transplantation is accepted as a valid and advanced treatment method applied in many chronic organ diseases. (tts.org)
MaR1 treatment was also protective against protease activation of MSCs under inflammatory conditions. (cdc.gov)
The MSC treatment was not associated with adverse events over the monitoring period. (lu.se)

We are interested in investigating the potential neuroprotective benefits of stem cell transplantation, which has produced encouraging results in different models of CNS degeneration. (nih.gov)

Collectively, our data indicate that MSC therapy rescues impaired mitochondrial morphology, enhances host metabolic capacity, and induces widespread host gene shifting. (frontiersin.org)

The search strategies included MeSH terms and other related terms of MSC-derived mediators and Mφs. (frontiersin.org)

You'll examine the biology and evidence base behind these conditions, find out how they are diagnosed and treated by clinicians, and learn from researchers who are developing cutting-edge new treatments such as gene therapy and stem-cell transplantation. (sheffield.ac.uk)

Unfortunately, organ shortage is the greatest challenge facing the field of organ transplantation today. (tts.org)

Proof of concept has been shown in animal models of TID, and safety and potency for immune suppressive capacities of autologous MSC already tested in humans. (uu.se)

and immunosuppression, such as those with hematopoietic stem cell transplantation and hematologic malignancies ( 1 - 4 ). (cdc.gov)

Anatomy23

Organisms12

Diseases48

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment104

Psychiatry and Psychology1

Phenomena and Processes30

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science4

Named Groups4

Health Care28

Vitro11

Liver22

Differentiation potential3

Bone marrow-derived2

Stromal4

Allogeneic1

Adipose2

Proliferation5

Differentiate5

Therapeutic potential4

Islet transplantation2

Vivo7

Access to transplantation2

Stem cells15

20231

Induce1

Cardiac transplantation2

Phenotype2

Paracrine3

Clinical7

Lung7

Transfusion1

Obtained from1

Tissue2

Integrin3

Pancreatic1

Interaction1

Graft2

Viable2

Thoracic1

Cells10

Donor2

Modulate2

Treatment4

Potential1

Metabolic1

MeSH1

Gene therapy1

Shortage1

Immune1

Immunosuppression1