Transplantation epithelial cells. Medical search. Frequent questions

Genetically engineered human retinal pigment epithelial cells for intraocular transplantation therapy. (ox.ac.uk)
In the next clinical trial," continues Sugita, "we plan to use allogeneic iPS-retinal pigment epithelial cells from HLA homozygote donors. (neurosciencenews.com)
This system shows great potential for development as a substitute Bruch's membrane, providing a synthetic, non-toxic, biostable support for transplantation of the retinal pigment epithelial cells. (aru.ac.uk)

Now that we have established the lack of immune response in monkeys and in human cells in vitro," explains Sugita, "using the iPS cell bank appears to be a viable solution, at least in the case of retinal pigment epithelial cell transplantation. (neurosciencenews.com)

Pathologic examination of a bronchoalveolar lavage (BAL) sample showed scattered cells, primarily columnar bronchial cells, with cytomorphologic changes reminiscent of BK polyomavirus (BKPyV)-infected decoy cells. (cdc.gov)
Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
When primary isolated human bronchial epithelial cells were treated with 1-100 nM MaR1, transforming growth factor (TGF)-B1-induced EMT processes were altered. (cdc.gov)
Furthermore, when conditioned medium from DHA-treated MSCs was used to culture bronchial epithelial cells, the epithelial cells exhibited modulation of protease activation. (cdc.gov)
Our results identify DHA and MaR1 as regulators of reparative processes after lung inflammation and wounding, including effects on two pivotal cellular players-bronchial epithelial cells and MSCs. (cdc.gov)
To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-κB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-κB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-κB in non-CF and CF human bronchial gland epithelial cells. (ersjournals.com)
It was demonstrated that a relevant concentration of FP (10 −8 M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. (ersjournals.com)
Interestingly, the expression of two IκB kinases (IKK)-α/β, the degradation of cytosolic IκB-β inhibitor and the NF-κB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. (ersjournals.com)
It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IκB-α/β kinases in bronchial epithelial cells. (ersjournals.com)
In particular the strong effect on the IκB-β kinase, which is known to be elevated in bronchial epithelial cells in cystic fibrosis patients, was observed. (ersjournals.com)
The present authors and previous studies, have previously demonstrated that epithelial expression of interleukin (IL)-8 is excessively elevated in the bronchial epithelial cells of human CF adults 6 . (ersjournals.com)
CC16 levels correlate with cigarette smoke exposure in bronchial epithelial cells and with lung function decline in smokers. (medscape.com)
RNAseq analysis of bronchial epithelial cells to identify COPD-associated genes and SNPs. (medscape.com)

An allogeneic source of transplantable cells for use in total bilateral disease can be obtained from culture of donated cadaveric corneal tissue. (liverpool.ac.uk)
We performed a controlled multicenter study to examine the feasibility, safety, and efficacy of allogeneic corneal epithelial stem cells in the treatment of bilateral LSCD. (liverpool.ac.uk)
Patients were randomized to receive corneal epithelial stem cells cultured on amniotic membrane (AM): investigational medicinal product (IMP) or control AM only. (liverpool.ac.uk)
This first randomized control trial of allogeneic corneal epithelial stem cells in severe bilateral LSCD demonstrates the feasibility and safety of this approach. (liverpool.ac.uk)
To characterize the phenotype of human limbal epithelial cells expanded on a standardized biological substrate with regard to morphology, growth potential, and antigen expression patterns, and to evaluate their clinical application in corneal surface reconstruction. (arvojournals.org)
Human corneal limbal specimens were obtained from 10 donor eyes and expanded by explant culture on fibrin gels with 3T3 feeder cells. (arvojournals.org)
After standardization of the technique, fibrin-cultured autologous epithelial sheets were transplanted onto the corneal surface of 5 patients with limbal stem cell deficiency due to chemical burns. (arvojournals.org)
After transplantation of autologous fibrin-cultured limbal cells, the patients' corneas showed complete epithelialization and a decrease in corneal scarring and vascularization by 2 weeks following surgery. (arvojournals.org)
To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
This characteristic of human corneal epithelium may explain, at least in part, why amniotic membrane transplantation is proving to be clinically useful for human ocular surface reconstruction. (nih.gov)
Transplantation of autologous corneal epithelial stem/progenitor cells (CESCs) expanded in culture has successfully restored vision and revolutionized the treatment of limbal stem cell deficiency (LSCD), which is a major cause, either primary or secondary, of significant visual loss and blindness in many corneal disorders including aniridia. (uclahealth.org)
Because the maintenance of stem cell characteristics in the process of cell culture expansion is essential for the success of ocular surface reconstruction, the small molecules generated in this project may also be helpful in the development of novel pharmaceutical reagents for treating other corneal epithelial disorders. (uclahealth.org)

At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
The phenotypes of primary cultured cell sheets were evaluated by light and electron microscopy and by immunohistochemical staining with antibodies for putative stem cell markers, differentiation markers, basement membrane markers, and adhesion molecules. (arvojournals.org)
CCN1 also induced Jag1 expression in hepatic stellate cells, whereupon they interacted with hepatic progenitor cells to promote their differentiation into cholangiocytes. (jci.org)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
Toward this goal, we propose to compare the behavior of nerve cell differentiation in multiple lines of hESCs in one laboratory setting. (ca.gov)
We will further characterize molecular changes during directed cell differentiation and identify the cells that exhibit a pattern of DNA modification, namely DNA methylation, similar to primary neural cells in human brain. (ca.gov)
We are now ready to compare the property of different lines of nerve cells such as the efficiency of nerve cell differentiation and the preferential production of specific nerve cells in culture. (ca.gov)
By engineering stem cells carrying different levels of methylation, we aim to find the optimal levels of DNA methylation for efficient nerve cell differentiation. (ca.gov)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
Study author Professor Barbara Pierscionek, Deputy Dean (Research and Innovation) at Anglia Ruskin University (ARU), said: "This research has demonstrated, for the first time, that nanofibre scaffolds treated with the anti-inflammatory substance such as fluocinolone acetonide can enhance the growth, differentiation, and functionality of RPE cells. (aru.ac.uk)
Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. (lu.se)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

These findings are important in the future development of ocular tissue for transplantation into the patient's eye. (aru.ac.uk)
In particular, our work focuses on the design and use of biologic and synthetic scaffolds to bioengineer new lung tissue for transplantation. (lu.se)

HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. (thermofisher.com)
Human hematopoietic cells and thymic epithelial cells induce tolerance via different mechanisms in the SCID-hu mouse thymus. (rupress.org)
Immunohistology of the thymus grafts demonstrated that thymic epithelial cells were of fetal thymus donor (FTD) origin. (rupress.org)
These data demonstrate that T cells become tolerant to autologous and allogeneic HLA antigens expressed in the thymus via two different mechanisms: hematopoietic cells present in the thymus induce tolerance to "self"-antigens by clonal deletion, whereas thymic epithelial cells induce tolerance by clonal energy and possibly deletion of high affinity clones. (rupress.org)

Any problems that might arise in the case of allogeneic cells, which are donated by someone other than the patient, still need to be determined. (medicalxpress.com)
Recent clinical trials evaluating allogeneic retinal grafts derived from human embryonic stem cells (hESCs) show the procedure to be safe and potentially effective 1 . (nature.com)
Thymocytes of SCID-hu mice transplanted with liver and thymus of two different donors (FLDA/FTDB animals) were nonresponsive to Epstein-Barr virus-transformed B cell lines (B-LCL) established from both the FLDA and FTDB, but proliferated vigorously when stimulated with third-party allogeneic B-LCL. (rupress.org)

Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

FP induces apoptosis of eosinophils 18 and also reduces the production of several cytokines such as IL-1β, IL-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) by alveolar macrophages 19 and lymphocytes 20 , 21 . (ersjournals.com)
To analyze the mechanisms responsible for the Ron-dependent regulation of cytokine/chemokine production, the laboratory utilizes co-culture systems composed of primary tissue macrophages and epithelial cells from select gene knockout animals to dissect the role of macrophages in tissue injury. (uc.edu)
Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
Dendritic cells and macrophages of fetal liver donor (FLD) origin were abundantly present in the medullary and cortico-medullary areas. (rupress.org)
some is secondarily produced by alveolar macrophages and epithelial cells. (msdmanuals.com)

Limbal stem cell deficiency (LSCD) is a disease resulting from the loss or dysfunction of epithelial stem cells, which seriously impairs sight. (liverpool.ac.uk)
People who experience chemical burns and other eye injuries may develop limbal stem cell deficiency, an irreversible loss of cells on the tissue surrounding the cornea. (worldhealth.net)

In summary, we demonstrate a role of motile cilia in TGF-β1 sensing and showed that TGF-β1 disturbs TJ permeability of conductive airway epithelia by redistributing CLDN3 from TJs into cell nuclei. (springer.com)
During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
Lung transplantation-related pathology encompasses a spectrum of disorders that include, but are not limited to, indications for lung transplantation (seen in explanted lungs), surgical complications (airway anastomotic and vascular complications), ischemia-reperfusion injury, rejection (acute and chronic), infections, and posttransplantation lymphoproliferative disorders (PTLDs). (medscape.com)
Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. (ersjournals.com)
In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-κB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. (ersjournals.com)
Cystic fibrosis (CF) is a lethal inherited disorder caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) in airway epithelial cells 1 . (ersjournals.com)
One of the major clinical manifestations of CFTR mutations is neutrophil dominated inflammation 2 , 3 , implying that defective CFTR affects the immune function of airway epithelial cells, in particular the production of nuclear factor (NF)-κB-dependent chemokines. (ersjournals.com)
How CFTR dysfunction in CF airway cells contributes to the activation and nuclear localisation of NF-κB is still unclear. (ersjournals.com)
Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airway disease. (biovendor.com)
Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. (lu.se)

We tested the hypothesis that the stromal cells from PZ and transitional zone (TZ) have differential effects on the ability of tumorigenesis. (jcancer.org)
Stromal cells isolated from the PZ and TZ of normal human prostates mixed with DU145 cells subcutaneously injected into athymic nude mice. (jcancer.org)
The C-Kit inhibitor, imatinib mesylate, was administrated to confirm the effect of stromal cells on the tumorigenesis. (jcancer.org)
The volume and weight of tumors were significantly higher in mice transplanted with DU145 and stromal cells from PZ. (jcancer.org)
In contrast, the data was significantly lower with DU145 and stromal cells from TZ than DU145 alone. (jcancer.org)
The purified DU145 cells isolated from the tumors with DU145 and stromal cells in PZ had increased ability to migrate and proliferate, and had increased expression of C-Kit. (jcancer.org)
These effects of the stromal cells in the PZ on DU145 cells could be blocked using imatinib mesylate. (jcancer.org)
in contrast, the stromal cells in the TZ inhibit it through down-regulating the expression of C-Kit. (jcancer.org)
Interestingly, MaR1 also regulated the actions of primary isolated human lung mesenchymal stem (stromal) cells (MSCs). (cdc.gov)
Future studies will involve the utilization of in vivo gene-targeted murine models to study disease progression as well as the isolation and analysis of primary epithelial and stromal cells from the breast and prostate environments. (uc.edu)
The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
Mesenchymal stromal cells (MSCs) have shown benefit in other inflammatory diseases. (unav.edu)

Pterygium excision combined with autologous conjunctival flap transposition, pterygium excision combined with amniotic membrane transplantation, pterygium excision combined with autologous limbal stem cell transplantation and so on [12] . (researchsquare.com)

An amniotic epithelial cell is a form of stem cell extracted from the lining of the inner membrane of the placenta. (wikipedia.org)
Different epithelial membrane zone components have been recognized by antibodies in patients with mucous membrane pemphigoid, including BP antigens 1 and 2 (BP230 and BP180), laminin-332, laminin-311, type VII collagen, b4 integrin subunit, and antigens with unknown identities (a 45-kd protein, uncein, a 168-kd epithelial protein, and a 120-kd epithelial protein). (medscape.com)
Much research focuses on developing a synthetic substrate in order to transplant healthy cells to the native Bruch's membrane (BM), however, the diseased native BM and related structures still leave potential for transplanted cells to succumb to disease. (frontiersin.org)
The apical surface of the membrane was cultured with ARPE-19 cells and the underside was decorated with poly(lactic acid-co-glycolic acid) (PLGA) or poly(glycolic acid) (PGA) degradable nanoparticles by electrospraying. (frontiersin.org)
ARPE-19 cells were able to form a monolayer on the surface of the membrane and no cell invasion into the membrane was seen. (frontiersin.org)
The basal surface of the cells break through the basement membrane and infiltrates and grows into the matrix. (researchsquare.com)
AMD can be caused by changes in the Bruch's membrane, which supports the RPE cells, and breakdown of the choriocapillaris, the rich vascular bed that is adjacent to the other side of the Bruch's membrane. (aru.ac.uk)

These cells are known to have some of the same markers as embryonic stem cells, more specifically, Oct-4 and nanog. (wikipedia.org)
In harvesting embryonic stem cells, a human embryo is destroyed. (wikipedia.org)
Thus their use averts the controversy about embryonic stem cells. (wikipedia.org)
Unlike embryonic stem cells, amniotic stem cells have not shown a propensity for developing into teratomas and other cancer-like tumors upon injection into living tissue. (wikipedia.org)
Amniotic epithelial cells have not been known to produce cancerous tumors in the host despite the fact that these cells do express genes found in embryonic stem cells that are known to promote tumor formation. (wikipedia.org)
During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)

Fibrin gels, therefore, represent a standardized and efficient substrate for adhesion, proliferation, and maintenance of limbal epithelial cells for successful clinical application. (arvojournals.org)
Importantly, the regenerating epithelium around the DUM demonstrated heightened STAT3 phosphorylation and cell proliferation, which was suppressed in uteri of Stat3 conditional knockout mice. (jci.org)
It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)

Mitsuru Mizuno et al, Transplantation of human autologous synovial mesenchymal stem cells with trisomy 7 into the knee joint and 5 years of follow‐up, STEM CELLS Translational Medicine (2021). (medicalxpress.com)
BACKGROUND: In 2021, four patients who had received solid organ transplants in the USA developed encephalitis beginning 2-6 weeks after transplantation from a common organ donor. (cdc.gov)

Conducted by a multi-institutional team of researchers in Japan who had developed the new therapy, the study was designed to confirm that their treatment-which involves transplanting the patient's own mesenchymal stem cells (MSCs) into the affected knee-did not cause tumors. (medicalxpress.com)
Bioactive lipid mediators regulate lung epithelial and mesenchymal cell reparative processes in vitro. (cdc.gov)
Lung wound-healing responses such as the epithelial-to-mesenchymal transition (EMT) can be reparative or pathologic processes. (cdc.gov)
Both in animal models and humans, it has been shown that cell therapy can induce therapeutic angiogenesis, making mesenchymal stromal cell-based therapy one of the most promising therapeutic alternatives. (unav.edu)

We tested whether CRISPR could precisely edit the pathogenic mutation and produce gene-corrected iPSCs for eventual use in autologous transplantation. (nature.com)

For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)

Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. (biovendor.com)

The basic research for the establishment of the donor for the neural transplantation with the miniature swine neuroepitherial stem cells. (nii.ac.jp)

Amniotic epithelial cells have shown to be safe and successfully transplanted into hosts that are not related to the donor. (wikipedia.org)
One possible reason for this is that amniotic epithelial cells have low antigen levels that inhibit compatibility from a donor to the recipient. (wikipedia.org)
Unlike traditional tissue transplants, where the donor and recipient have to be in complete sync as far as blood type and overall compatibility and even still can show tissue rejection, amniotic epithelial cells can be used and show a higher likelihood of tissue acceptance. (wikipedia.org)
Outgrowth of epithelial cells from all explants, regardless of donor age, was seen after 3-4 days on fibrin and reached confluence in 14-21 days. (arvojournals.org)
We present here a case of a 70-year-old woman with extensive eruptive syringomas that developed after deceased donor liver transplantation (DDLT). (medicaljournals.se)
The transplantation of a "foreign" organ, despite meticulous matching for donor-recipient compatibility, leads to a complex adaptive and innate immune system-mediated injury. (medscape.com)
Strategies We gathered both major IE and RPE- cells from 5 newly iced individual donor eye, using laser dissection microscopy and excision respectively. (woofahs.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)

The loose connective tissue under the epithelium contains a large number of fibroblasts and abundant capillaries, which indicates that the pterygium epithelial cells have a strong ability to proliferate and migrate. (researchsquare.com)
These organoids include a polarized epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial arrangement that mimic what is observed in vivo. (stemcell.com)
The intestinal epithelium incorporates several distinct cell populations, including the rapidly dividing intestinal stem cells (ISCs) that facilitate the typical four-to-five day turnover cycle of the adult intestinal epithelium. (stemcell.com)
Diagram of the small intestinal epithelium highlighting the identity and spatial arrangement of key epithelial cell types. (stemcell.com)
The adult intestinal epithelium is primarily composed of six cell types that are arranged in a crypt-villus structure (Figure 1). (stemcell.com)

Additionally, we are studying the isolation, culture, and mechanical property characterization of cells residing within the trabecular meshwork, an ocular tissue responsible for intraocular pressure homeostasis. (uclahealth.org)
Autologous intra-ocular RPE transplantation was previously carried out with limited success [1,2], since surgical variability and complications remained high. (woofahs.com)

This study highlights the ability of a patient's own stem cells to potentially heal torn cartilage in the knee," said Anthony Atala, M.D., Editor-in-Chief of STEM CELLS Translational Medicine and director of the Wake Forest Institute for Regenerative Medicine. (medicalxpress.com)
Discussion and conclusion Extensive fascial resection combined with autologous limbal stem cell sharing transplantation for the treatment of pterygium, the patient's appearance satisfaction is higher, and the recurrence probability is lower than the average recurrence rate of limbal stem cell transplantation. (researchsquare.com)
Using a patient's own cells for transplantation would avoid these pitfalls and is possible with induced pluripotent stem cells (iPSCs). (nature.com)
In CALEC, stem cells from a patient's healthy eye are removed via a small biopsy and then expanded and grown on a graft via an innovative manufacturing process at the Connell and O'Reilly Families Cell Manipulation Core Facility at Dana-Farber Cancer Institute. (worldhealth.net)
Existing treatment strategies have limitations and associated risks the CALEC procedure aims to address through its unique approach of using a small amount of a patient's own stem cells that can then be grown and expanded to create a sheet of cells that serves as a surface for normal tissue to grow back. (worldhealth.net)

Finally, cell transplantation in a mouse stroke model will be used to study the mechanisms and efficacy of different types of hESC-derived neural cells in neural repair. (ca.gov)
Lung transplantation is the only option at end-stage disease and is further complicated by shortage in organs available for transplantation and low efficacy. (lu.se)

A 28-year-old woman with Job syndrome was seen at the NIH Clinical Center 6 months after bilateral lung transplantation. (cdc.gov)
The clinical data after the transplantation will allow us to see if the iPS cell bank is truly useful or not. (neurosciencenews.com)
These results have been applied in clinical practice to establish ABO-incompatible renal and liver transplantation programs in Gothenburg and Birmingham, UK. (gu.se)
Future mechanistic and therapeutic investigations are warranted to ascertain the clinical usefulness of these lipids in regulating lung repair processes relevant to lung transplantation. (cdc.gov)
The clinical trial represents the first human study of a stem cell therapy to be funded by the National Eye Institute (NEI), a part of the National Institutes of Health (NIH). (worldhealth.net)
According to the authors, despite landmark studies describing an autologous stem cell approach over the past 25 years and similar methods being utilized in Europe, no U.S. research team had successfully developed a manufacturing process and quality control tests that met U.S. Food and Drug Administration (FDA) requirements or showed any clinical benefit. (worldhealth.net)
Clinical features of lung transplantation-related pathology can range from an absence of symptoms to signs and symptoms of respiratory distress and/or infection and are not detailed in this article. (medscape.com)
The Registry of the International Society for Heart and Lung Transplantation (ISHLT) monitors and reports statistics on clinical lung transplantation. (medscape.com)
Also, the multidisciplinary coordination exercise needed to develop this clinical trial protocol will undoubtfully be useful to conduct academic clinical trials in the field of cell therapy in the near future. (unav.edu)
Journal of Investigational Allergology and Clinical Immunology is indexed/abstracted in Chemical Abstracts, Current Biology, Current Contents - Clinical Medicine, Database Subidase, Excerpta Medica - Immunology, Serology and Transplantation EMBASE, Index Medicus - Medline/Medlars, IBECS, Pascal INIST and Science Citation Index and its impact factor reaches 7.2 (2022) . (jiaci.org)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

The diagnostic difficulty of the present case owed to the fact that the patient was referred 11 years after liver transplantation and each lesion was larger than a typical syringoma. (medicaljournals.se)
To our knowledge, this is the first case of eruptive syringoma occurring after liver transplantation. (medicaljournals.se)

Apart from the current case, a case of eruptive syringoma that developed after kidney transplantation has been reported (6). (medicaljournals.se)
Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. (thermofisher.com)

Our data combined with that of the literature, represent a most comprehensive perspective on transcriptional variation, which may support future research in the development of therapeutic transplantation of IE. (woofahs.com)
Summary of Research Progress: Our research aims to identify the optimal culture conditions and the best hESC lines for the derivation of nerve lineage cells in therapeutic cell transplantation. (ca.gov)
The replacement of the RPE cells is among several promising therapeutic options for effective treatment of sight conditions like AMD, and researchers have been working on efficient ways to transplant these cells into the eye. (aru.ac.uk)

Tissues obtained from amniotic cell lines show promise for patients with congenital diseases or malformations of the heart, liver, lungs, kidneys, and cerebral tissue. (wikipedia.org)
A thorough examination of saposin D-mutated PD patient-derived cells from the Japanese patients reported endoplasmic reticulum PSAP retention but preserved sphingolipid hydrolase activity, which is inconsistent with the canonical theory of PSAP and saposins 7 . (nature.com)
The results showed that five years after transplantation, osteoarthritis-related tears to the knee meniscus had healed and, just as importantly, none of the patients experienced any serious side effects from the treatment. (medicalxpress.com)
We recruited 10 patients for the study and transplanted their own stem cells into the affected knee joints , then followed up with MRIs over the next five years. (medicalxpress.com)
The images revealed that tears in the patients' knee meniscus were obscured three years after transplantation. (medicalxpress.com)
Methods A retrospective case series analysis was performed with 403 patients (420 eyes) who underwent extensive fascial resection combined with autologous limbal stem cell sharing transplantation in the Department of Ophthalmology, Second People's Hospital of Yunnan Province from January 2017 to June 2018. (researchsquare.com)
Cultivated autologous limbal epithelial cells (CALEC) procedure shown to be safe and feasible with early positive results of restored cornea surfaces or vision gains in four patients with severe chemical burns. (worldhealth.net)
A team led by researchers from Mass Eye and Ear, a member of Mass General Brigham, reports the results of a phase I trial of a revolutionary stem cell treatment called cultivated autologous limbal epithelial cell transplantation (CALEC), which was found to be safe and well-tolerated over the short term in four patients with significant chemical burns in one eye. (worldhealth.net)
Without limbal cells and a healthy eye surface, patients are unable to undergo artificial cornea transplants, the current standard of vision rehabilitation. (worldhealth.net)
NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
The International Society for Heart and Lung Transplantation is a not-for-profit, multidisciplinary professional organization dedicated to improving the care of patients with advanced heart or lung disease through transplantation, mechanical support and innovative therapies. (ishlt.org)
Ninety eligible patients will be randomly assigned at a ratio 1:1:1 to one of the following: control group (n = 30), low-cell dose treatment group (n = 30), and high-cell dose treatment group (n = 30). (unav.edu)
In a first escalating-dose phase, three patients were included sequentially in three dose cohorts (10×106, 50×106 and 100×106 cells). (unav.edu)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
BACKGROUND: Recent reports with a small number of patients showed an association of red blood cell distribution width (RDW) with prostate cancer (PCa) progression. (bvsalud.org)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
These cells are heavily damaged in patients with lung fibrosis. (lu.se)
Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)

The bile ducts carry bile, a fluid that is produced by the liver cells (hepatocytes) and modified by the biliary lining (epithelial) cells as it flows through the ducts to the small intestine. (emedicinehealth.com)

Because there is not a sufficient amount of amniotic epithelial cells extracted from a single term placenta to be use in cellular therapies, the cells must be expanded in a lab. (wikipedia.org)
One prospective use of these cells includes cellular therapies aimed at dropping inflammation and scarring. (wikipedia.org)
This important proof-of-concept finding supports the development of personalized iPSC-based transplantation therapies for retinal disease. (nature.com)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

The team behind the current study, which included researchers from Tokyo Medical and Dental University, Kyoto University and Kazusa DNA Research Institute, recently developed a therapy involving the transplantation of MSCs derived from the knee's soft tissue (the synovium) into the injured meniscus. (medicalxpress.com)
MSCs are multipotent adult stem cells present in the umbilical cord, bone marrow, fat, dental and other body tissues. (medicalxpress.com)
Nevertheless, we believe that these data suggest that MSCs with trisomy 7 are safe for transplantation into human knees and show much promise in treating osteoarthritis. (medicalxpress.com)

The pluripotency of the human amniotic epithelial cells makes them useful in treating and fighting diseases and disorders of the nervous system as well as other tissues of the human body. (wikipedia.org)
By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)
These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)
One of the most promising future therapy options for RPE related disorders is usually cell replacement of the dysfunctional RPE. (woofahs.com)
Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)

More specifically, amniotic epithelial cells have been used in the past to treat genetic liver diseases such as ornithine transcarbamylase deficiency, familial hypercholesterolemia, and Crigler-Najjar syndrome. (wikipedia.org)

Interestingly, the first genome-wide CRISPR interference/activation screens to discover susceptibility genes of oxidative stress in induced pluripotent stem cell (iPSC)-derived human neurons identified PSAP. (nature.com)
Notes lead author Sunao Sugita, "In order to make iPSC transplantation a practical reality, the current goal is to create banks of iPSC-derived tissues that can be transplanted into anyone as they are needed. (neurosciencenews.com)
In vitro, the team saw that T cells failed to respond to the iPSC-derived retinal pigment cells if they were from an MHC-matched monkey. (neurosciencenews.com)
Therefore, many studies in the last decade focused on the development and use of induced pluripotent stem cells (iPSC) as a source for autologous cell replacement therapy. (woofahs.com)

Stromal and myometrial regeneration occurred following epithelial regeneration. (jci.org)
In ovariectomized mice, uterine regeneration of the DUM transplantation was similarly observed, suggesting that ovarian hormones are not essential for this regeneration process. (jci.org)
The DUM transplantation model is a powerful tool for uterine regeneration research. (jci.org)
17 This property of rapid regeneration at intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both inside and outside the specific context of intestinal function. (stemcell.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)

Placental stem cells. (scielo.org.ar)

The detection of trisomy 7 in epithelial cells has been associated with tumor formation. (medicalxpress.com)

Immunohistochemical examination showed that infiltration by inflammatory cells was only present in the transplanted grafts of MHC-mismatched monkeys. (neurosciencenews.com)
To generate healthy patient-derived cells, mutations might be repaired with new gene-editing technology based on the bacterial system of clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9, thereby yielding grafts that require no patient immunosuppression. (nature.com)
It was challenging to develop a process for creating limbal stem cell grafts that would meet the FDA's strict regulatory requirements for tissue engineering," said Ritz, executive director of the Connell and O'Reilly Families Cell Manipulation Core Facility at Dana-Farber and professor of medicine at Harvard Medical School. (worldhealth.net)

Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)
Recently, an in vitro study on the expression of ΔF508 CFTR (CFTR which contains a deletion of phenylalanine at position 508) in the ovary cells of Chinese hamsters, which do not express CFTR, revealed a seven-fold increase in the activation of NF-κB compared to the wild-type CFTR or the G551D mutant. (ersjournals.com)
Some of these techniques include novel tools for genetic manipulation, 4,5 approaches for in vitro disease modelling 6-9 and innovative co-culture system with autologous cell types 10,11 or bacteria, 12-14 as well as viral infection models. (stemcell.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)

The effects of corticosteroids are predominantly mediated by the inhibition of NF-κB deoxyribonucleic acid (DNA)-binding activity, which controls genes encoding inflammatory cytokines, chemokines and adhesion molecules in respiratory epithelial cells 10 - 13 . (ersjournals.com)
Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)

It is the first time this technology, called 'electrospinning', has been used to create a scaffold on which the RPE cells could grow, and could revolutionise treatment for one of age-related macular degeneration, one of the world's most common vision complaints. (aru.ac.uk)

This makes the possibility of rejection of the cells by the recipient tissue less likely. (wikipedia.org)
Summary: Researchers report they have successfully transplanted retinal pigment cells derived from stem cells without rejection or the need of immunosuppressant drugs. (neurosciencenews.com)
Researchers at the RIKEN Center for Developmental Biology (CDB) have successfully transplanted retinal pigment cells derived from stem cells of one monkey into the eyes of other monkeys without rejection and without the need for immunosuppressant drugs. (neurosciencenews.com)
In order to avoid tissue rejection, they used autologous iPSCs-iPSCs that were created from the recipient's own skin cells. (neurosciencenews.com)
The researchers found that these transplanted cells survived without rejection for at least 6 months in MHC-matched monkeys, without using any of the usually necessary immunosuppressant drugs. (neurosciencenews.com)
Although aimed at protecting the host from infection, in the context of transplantation, this injury leads to allograft rejection. (medscape.com)

Also, these lab grown cells have shown altered gene expression causing a differing phenotype as well as differing antigen levels. (wikipedia.org)
Over 500 products have been investigated for neuroprotective effects including those from the categories of free radical scavengers, anti-excitotoxic agents, apoptosis (programmed cell death) inhibitors, anti-inflammatory agents, neurotrophic factors, metal ion chelators, ion channel modulators, and gene therapy. (researchandmarkets.com)
In eukaryotic cells, chromatin comprises a complex consisting of DNA, RNA, and proteins where covalent modifications correlate with regulation of gene expression [ 1 ]. (biomedcentral.com)
In addition, the laboratory plans to use orthotopic transplantation of gene-modified cell lines into syngeneic models to explore these signaling pathways in detail. (uc.edu)

Currently, adult cells can be reprogrammed into stem cells, and then re-differentiated and grown into desired cell types. (neurosciencenews.com)
Stem cells exist both in embryos and adult cells. (articlecity.com)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

The cells stained positive with PAb416, a monoclonal antibody against the SV40 large T antigen. (cdc.gov)

The etiology of lung transplantation-related pathology is almost always immune-mediated. (medscape.com)

After his PhD examination, his biochemical studies relating to ABO-incompatible organ transplantation continued in the Department of Surgery. (gu.se)
Recent infection with yellow fever virus was confirmed in all four organ recipients by identification of yellow fever virus RNA consistent with the 17D vaccine strain in brain tissue from one recipient and seroconversion after transplantation in three recipients. (cdc.gov)

Since the introduction of the mouse small intestinal organoid model in 2009, 1 there has been an avalanche of developments in this field, including development of culture conditions for human organoids derived from primary colonic tissue, 2 as well as from human pluripotent stem cells (hPSCs). (stemcell.com)
The intestinal niche has been well-characterized, and shown to consist of spatial gradients of high WNT and Epithelial Growth Factor (EGF), while Bone Morphogenetic Protein (BMP) signals are inhibited. (stemcell.com)
18 At the base of the intestinal crypt, the ISCs are found intercalated with Paneth cells, 17 credited with much of the signaling required to maintain the stem cell niche. (stemcell.com)
The antimicrobial protein BPI (Bactericidal Permeability Increasing protein) is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. (biovendor.com)

Anatomy50

Organisms13

Diseases38

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment82

Phenomena and Processes34

Disciplines and Occupations1

Information Science6

Named Groups3

Health Care16

Pigment epithelial cells3

Retinal pigment epithelial cell transplantation1

Bronchial13

Corneal15

Differentiation17

Tissue for transplantation2

Thymic epithelial cells4

Allogeneic3

Progenitor cells1

Macrophages5

Stem cell deficiency2

Airway12

Stromal cells12

Amniotic membrane transplantation1

Membrane7

Embryonic stem9

Proliferation3

20212

Mesenchymal4

Autologous transplantation1

Bone marrow transpla1

Hematopoietic1

Neural transplantation1

Donor8

Epithelium5

Ocular2

Patient's5

Efficacy2

Clinical14

Liver transplantation2

Kidney transplantation2

Therapeutic3

Patients20

Biliary1

Therapies7

MSCs3

Disorders5

Deficiency1

IPSC4

Regeneration5

Placental1

Tumor formation1

Grafts3

Vitro5

Adhesion2

Macular degeneration1

Rejection6

Gene4

Adult3

Antigen1

Immune-mediated1

Organ2

Intestinal4