Transplantation 1996 wilmut nuclear transfer techniques. Medical search. Frequent questions

In 1996, Dolly the sheep achieved notoriety for being the first mammal cloned from a somatic cell. (wikipedia.org)
Dolly the Sheep, the first mammal to be cloned from an adult derived somatic cell, was born in 1996. (nottingham.ac.uk)
Professor Sir Ian Wilmut, who worked with Professor Campbell on the creation of Dolly the Sheep, said: "Always cheerful and friendly, Keith will be greatly missed by all of his friends and colleagues. (nottingham.ac.uk)
Yamanaka also noted that experiments in cloning Dolly the sheep in 1996, conducted by Ian Wilmut, Angelica Schnieke, Jim McWhir, Alex Kind, and Keith Campbell at the Roslin Institute in Roslin, Scotland, influenced his work. (asu.edu)

The Dolly experiment showed that scientists could reprogram the nucleus of somatic cells by transferring the contents of the nucleus into oocytes that have had their nuclei removed, a technique called somatic cell nuclear transfer (SCNT). (asu.edu)
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)

Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (chinagene.cn)
Even advanced donor cells from the endoderm of Xenopus tadpoles have nuclei that can sometimes yield normal individuals after nuclear transfer [data taken from Briggs and King ( Briggs and King, 1957 ) for Rana and from Gurdon ( Gurdon, 1962 ) for Xenopus ]. (biologists.com)
These observations suggest that further studies on nuclear reprogramming are needed in order to understand the underlying mechanisms of reprogramming and significantly improve the ability of the differentiated somatic nuclei to be reprogrammed. (biomedcentral.com)

is a British developmental biologist who was the first to use nuclear transfer of differentiated adult cells to generate a mammalian clone, a Finn Dorset sheep named Dolly, born in 1996. (mathisfunforum.com)
Following two postdoctoral positions he joined the Roslin Institute in Scotland in 1991, where he applied his previous experience to the production of mammalian embryos by nuclear transfer. (nottingham.ac.uk)
His research blossomed after he came to Roslin Institute where in a series of papers he put the intellectual framework into the method of mammalian cloning that ultimately led to the birth of Dolly in 1996. (nottingham.ac.uk)

This article summarizes the history and current situation in fish nuclear transfer tech-nology and discusses the factors that may influence the development of the cloned embryos. (chinagene.cn)
Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)

The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. (wikipedia.org)

A comprehensive understand-ing of the mechanism for epigenetic modification following nuclear transfer, such as genomic DNA methylation and histone acetylation and/or methylation, will likely increase the success rate and eventually lead to the future freedom of cloning technique. (chinagene.cn)

Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
It demonstrated that genes inactivated during tissue differentiation can be completely re-activated by a process called nuclear reprogramming: the reversion of a differentiated nucleus back to a totipotent status. (biomedcentral.com)

Much of this research was presented and discussed at the annual meeting of the International Embryo Transfer Society where he will be remembered as an enthusiastic participant in discussions on current topics. (nottingham.ac.uk)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)

Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. (wikipedia.org)
More than 100 nuclear transfer procedures could be required to produce one viable clone. (wikiquote.org)

Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)

Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)

Natural cloning is the production of clones without the involvement of genetic engineering techniques. (wikipedia.org)

12] Lee KY, Huang HG, Ju BS, Yang ZG, Lin S. Cloned ze-brafish by nuclear transfer from long-term-cultured cells. (chinagene.cn)

This Spotlight article is a commentary on the early nuclear transplant work in Xenopus , which was very important for the Nobel award in 2012, and the influence of this work on the reprogramming field. (biologists.com)

As an important sub-field in the study of animal cloning, fish nuclear transfer was first established in the early 1960s by Chinese embryologists. (chinagene.cn)