Transmembrane saccharomyces cerevisiae proteins. Medical search. Frequent questions

In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)
We have used the pheromone alpha-factor receptor (Ste2p) of the yeast Saccharomyces cerevisiae as a model system to understand ligand binding, receptor activation, and G protein interaction. (tennessee.edu)
The hydrophobic, but not the hydrophilic, domain of plant CHX is remarkably similar to monovalent cation/proton antiporter-2 (CPA2) proteins, especially yeast (Saccharomyces cerevisiae) KHA1 and Synechocystis NhaS4. (umn.edu)
In the yeast Saccharomyces cerevisiae , which is widely applied for industrial bioethanol production, uptake of hexoses is mediated by transporters with a facilitated diffusion mechanism. (biomedcentral.com)
To understand the molecular biology of peptide hormone action, we studied response of budding yeast ( Saccharomyces cerevisiae ) to its peptide mating pheromones ( a -factor and α-factor). (berkeley.edu)
Much of our work is conducted in the yeast Saccharomyces cerevisiae, an organism that permits both facile genetic manipulation and detailed biochemical analysis. (yale.edu)
The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. (uni-goettingen.de)
This is illustrated by its role in polarized cell growth in the yeast Saccharomyces cerevisiae . (rupress.org)

Saccharomyces cerevisiae (commonly known as baker's yeast) is a single-celled eukaryote that is frequently used in scientific research. (jove.com)
Saccharomyces cerevisiae , otherwise known as baker's yeast, is one of the many model organisms studied in laboratories all over the world. (jove.com)

The Saccharomyces cerevisiae EMP47 gene encodes a nonessential type-I transmembrane protein with sequence homology to a class of intracellular lectins defined by ERGIC-53 and VIP36. (rupress.org)
hZIP4 encodes eight transmembrane domains and a large extracellular domain (ECD). (bvsalud.org)
FGFR 3 encodes a transmembrane tyrosine kinase receptor involved in signaling via interaction with the family of fibroblast growth factors. (aacrjournals.org)

The size of the central cavity of CadB and PotE is dependent on the angle of transmembrane helix 6 (TM6) against the periplasm. (tcdb.org)
Evidence from statistical cluster analyses of a multiple sequence alignment of G protein-coupled receptor seven-helix folds supports the existence of structurally conserved transmembrane (TM) ligand binding sites in the opioid/opioid receptor-like (ORL1) and amine receptor families. (eurekamag.com)
Rhomboids are an ancient and conserved family of intramembrane-cleaving proteases, a small group of proteolytic enzymes capable of hydrolyzing a peptide bond within a transmembrane helix that anchors a substrate protein to the membrane. (ulaval.ca)
This function appears to have required the emergence of a structural feature that is unique among all other rhomboids: an additional transmembrane helix (TMH) positioned at the N-terminus of six TMHs that form the core proteolytic domain of all prokaryotic and eukaryotic rhomboids. (ulaval.ca)

Multi-pass membrane protein. (cusabio.com)
Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)
The di-lysine motif of Emp47p was functional when transplanted onto Ste2p, a plasma membrane protein, conferring ER localization. (rupress.org)
This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
We demonstrate the successful application of this protocol for the Met-Ser containing membrane protein fusion OmpX-Om14. (praiseworthyprize.org)
Cleavage of the Om14 (the yeast mitochondrial outer membrane protein) from E. coli OmpX (Outer membrane protein X) has provided yields of up to 70% product without detectable degradation levels. (praiseworthyprize.org)

Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
The predicted protein (85-95 kD) of 28 AtCHX genes after revision consists of an amino-terminal domain with 10 to 12 transmembrane spans (approximately 440 residues) and a hydrophilic domain of approximately 360 residues at the carboxyl end, which is proposed to have regulatory roles. (umn.edu)
1] "Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake. (tcdb.org)
This domain occurred 356 times on human genes ( 810 proteins). (umbc.edu)

For instance, how can cells control the diverse patterns of glycosylation of various secretory proteins in the endoplasmic reticulum and Golgi in an orderly manner with high accuracy? (go.jp)
Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)
7] "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. (tcdb.org)
We are currently studying a transmembrane ubiquitin ligase that resides in the nuclear envelope and endoplasmic reticulum. (yale.edu)
His Endoplasmic-reticulum-associated protein degradation and Endoplasmic reticulum study, which is part of a larger body of work in Cell biology, is frequently linked to Degradation, bridging the gap between disciplines. (research.com)
His studies deal with areas such as Ubiquitin, Ubiquitin ligase, Endoplasmic reticulum and Protein subunit as well as Protein degradation. (research.com)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
At the beginning of its establishment, KMD Bioscience started from the phage display technology platform to provide high-quality antibody discovery services, and gradually established stable cell line construction and screening, natural protein extraction and fermentation, and recombinant protein custom expression services, a multi-species antibody discovery platform. (kmdbioscience.com)

S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (jove.com)
Now that we've learned a bit about S. cerevisiae as an organism, let's discuss what makes it a great model system for research. (jove.com)

The Sty1/Hog1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)
The Wee1 class of protein-tyrosine kinase has an important role in cell cycle control. (berkeley.edu)
In EPH-related tyrosine kinases, appears to mediate cell-cell initiated signal transduction via the binding of SH2-containing proteins to a conserved tyrosine that is phosphorylated. (embl.de)

Biochemistry is represented through his Protein subunit, Saccharomyces cerevisiae, Ubiquitins, Biogenesis and Sumoylation Pathway research. (research.com)
His Proteasome research includes elements of Protein structure, Biogenesis, Protein subunit and Chaperone. (research.com)
Mark Hochstrasser focuses mostly in the field of Proteasome, narrowing it down to topics relating to Protein subunit and, in certain cases, Chaperone and Coiled coil. (research.com)
This dual sorting mechanism is currently being investigated, and in yeast appears to be regulated by a recently discovered subunit, the Mgr2 protein. (frontiersin.org)

They exhibit 7 putative transmembrane α-helical spanners (TMSs) and vary in size between about 200 and 500 amino acyl residues, although most have between 300 and 400 residues. (wikipedia.org)
An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
The sterile alpha motif (SAM) domain is a putative protein interaction module present in a wide variety of proteins [ ( PUBMED:9007998 ) ] involved in many biological processes. (embl.de)

Serine/Threonine Kinases (STKs), Fungal Mitogen-Activated Protein Kinase (MAPK) Sty1/Hog1 subfamily, catalytic (c) domain. (umbc.edu)
We investigated control mechanisms that regulate the activity, localization, and stability of Wee1, especially the bud neck-localized protein kinase Hsl1 and its more distant paralogs (Gin4 and Kcc4), in particular their recruitment to septin filaments, which assemble at the presumptive site of cell division. (berkeley.edu)
Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
Currently, four members of the FGFR family (FGFR1-4) have been identified which are composed of an extracellular Ig-like domain, a transmembrane domain, and an intracellular kinase domain (1) . (aacrjournals.org)
Par3 and Par6 bind one another and atypical protein kinase C (aPKC). (rupress.org)

Each GPCR has seven transmembrane domains and is in charge of sensing changes from the environment, transducing signals, and activating a series of biological responses. (tennessee.edu)

STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. (umbc.edu)
Residues that compose the RNA-binding surface are conserved in a subgroup of SAM domain-containing proteins, suggesting that the function of the domain is conserved from yeast to humans. (embl.de)

In such environments, the high extracellular glucose concentration can drive the uptake of glucose by yeast cells via diffusion, which is exemplified by the occurrence of many hexose transporters with a facilitated diffusion mechanism in S. cerevisiae . (biomedcentral.com)
Transmembrane and intracellular signal transduction mechanisms are the focus of our group, especially understanding how extracellular stimuli control cell growth and division, cell morphology, and gene expression at the biochemical level. (berkeley.edu)
Our studies are the first to show that an extracellular mucin-like protein plays an important role in animal osmoregulation in a manner that requires the activity of a novel transmembrane protein. (prolekarniky.cz)

Smaug, a protein that helps to establish a morphogen gradient in Drosophila embryos by repressing the translation of nanos (nos) mRNA, binds to the 3' untranslated region (UTR) of nos mRNA via two similar hairpin structures. (embl.de)
Anteroposterior patterning in Drosophila melanogaster is dependent on the sequence-specific RNA-binding protein Smaug, which binds to and regulates the translation of nanos (nos) mRNA. (embl.de)
The Nanos protein gradient in Drosophila, required for proper abdominal segmentation, is generated in part via translational repression of its mRNA by Smaug. (embl.de)

The lysosomal cystine transporter (LCT) family (TC# 2.A.43) is part of the TOG Superfamily and includes secondary transport proteins that are derived from animals, plants, fungi and other eukaryotes. (wikipedia.org)
These proteins are found in intracellular organelles of eukaryotes, many in lysosomes. (wikipedia.org)
Because many yeast proteins are similar in sequence and function to those found in other organisms, studies performed in yeast can help us to determine how a particular gene or protein functions in higher eukaryotes (including humans). (jove.com)
Interestingly, many proteins found in yeast share similar sequences with proteins from their fellow Eukaryotes. (jove.com)
By investigating the function of a given protein in yeast, researchers gain insight into the protein's function in higher eukaryotes, such as us, humans. (jove.com)
The G protein-coupled receptor (GPCR) family is composed of hundreds of members and is expressed in eukaryotes. (tennessee.edu)
Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)

All proteins in the LCT family are distantly related to the proteins of the microbial rhodopsin (MR) family (TC #3.E.1), an established member of the TOG Superfamily, which exhibit a 7 TMS topology. (wikipedia.org)
9] "A global topology map of the Saccharomyces cerevisiae membrane proteome. (tcdb.org)

A protein mutated in the rare human genetic disease, nephropathic intermediate cystinosis, also called cystinosin (TC# 2.A.43.1.1), is encoded by the CTNS gene. (wikipedia.org)
Genetic studies of osmoregulation in yeast have identified mucin-like proteins as critical regulators of osmosensitive signaling and gene expression. (prolekarniky.cz)
We are trying to understand the functional consequences of SUMO-protein modification, particularly in the cell cycle and chromatin-mediate gene transcription, and to determine the basis of specificity for the SUMO-cleaving proteases. (yale.edu)

The PI3K/Akt/mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. (go.jp)
In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
The signal transduction pathway of the receptor starts from sensing outside signal and then activates G proteins. (tennessee.edu)
These proteins take part in the fusion pathway in two consecutive steps. (centrodinoferrari.com)
The HOG pathway is mediated by two transmembrane osmosensors, Sln1 and Sho1. (umbc.edu)
We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)
The chloroplast albino 3 (ALB3) protein appears to integrate the light harvesting chlorophyll-binding protein into thylakoid membranes using a pathway that is distinct from the chloroplast Sec translocation pathway. (tcdb.org)

While ubiquitin is commonly used to mark its targets for destruction, the consequences of protein ligation to the various "ubiquitin-like proteins" are less understood. (yale.edu)
Ubiquitin and an array of related molecules (ubiquitin-like proteins or Ubls) such as SUMO are small, highly conserved proteins that are covalently attached to other intracellular proteins, resulting in various functional alterations of these targets. (yale.edu)

2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
Our study reveals the detailed molecular organization of the TOM complex and provides new insights about the mechanism of protein translocation into mitochondria. (escholarship.org)
Together, these suggest the dynamic nature of interactions within the subunits of the PAM complex, as well as between TOM and TIM23 subunits, during the process of translocation of a protein into mitochondria. (uni-goettingen.de)
Deletion of Mgr2p has been found to delay protein translocation into the matrix and accumulation in the inner membrane. (frontiersin.org)
The core of the TIM23 complex contains a pore that is essential for protein insertion into or translocation across the inner membrane. (frontiersin.org)

Both a wild-type and a mutant S. cerevisiae strain, in which the endogenous Zn2+ transporters were deleted, were used to test the expression and localization of an hZIP4-GFP fusion protein. (bvsalud.org)
Various classes of proteins and candidate molecules have been taken forward for clinical studies using our bacterial strain development capabilities. (premasbiotech.com)
S cerevisiae cell line is a PEP4/Prb1 protease deficient strain and has been consistently successful in expression of membrane proteins for study of target proteins. (premasbiotech.com)

A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)
It has been used for the production of reagent proteins, therapeutic recombinant proteins, including monoclonal antibodies. (premasbiotech.com)
Based on the above-mentioned major R&D platforms, KMD Bioscience has successfully developed many related reagents such as recombinant proteins, antibodies, antibody-drug similars, industrial enzymes, and diagnostic raw materials for scientific research and new drug discovery. (kmdbioscience.com)
J. C. Rodriguez, L. Wong, P. A. Jennings, The Solvent in CNBr Cleavage Reactions Determines the Fragmentation Efficiency of Ketosteroid Isomerase Fusion Proteins Used in the Production of Recombinant Peptides, Protein Expression and Purification, Volume 28, (Issue 2), April 2003, Pages 224-231. (praiseworthyprize.org)

A large pore is formed by transmembrane proteins. (tcdb.org)
Intrinsically disordered regions in Pex13 and Pex5 resemble those found in nuclear pore complex proteins. (tcdb.org)
Dimeric TOM consists of two copies each of five proteins arranged in two-fold symmetry: pore-forming β-barrel protein Tom40 and four auxiliary α-helical transmembrane proteins. (escholarship.org)
The gating of this protein-selective pore has been extensively studied in patch clamp experiments on isolated mitochondrial membranes. (frontiersin.org)

The proteasome is a molecular machine that fragments proteins into short peptides. (yale.edu)

Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. (frontiersin.org)
Based on the expectation that functionally conserved regions in homologous proteins will display locally higher levels of sequence identity compared with global sequence similarities that pertain to the overall fold, this approach may have wider applications in functional genomics to annotate sequence data. (eurekamag.com)

We found that the FERM domain in the tail region, which is known to bind to lipids as well as other proteins, is essential for both nuclear and chloroplast positioning, whereas the proximal MyTH4 domain plays a supporting role in chloroplast transport. (go.jp)
For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
The interacting domain between alpha-factor and transmembrane domain 2 to 3 of Ste2p was identified after DOPAC crosslinking, cyanogen bromide digestion and MALDI-TOF mass spectrometry. (tennessee.edu)
A full-length hZIP4-GFP and a truncated membrane-domain-only (mhZIP4-GFP) protein were observed to be present in the plasma membrane in yeast. (bvsalud.org)
Catalytic domain of the Serine/Threonine Kinases, Fungal Mitogen-Activated Protein Kinases Sty1 and Hog1. (umbc.edu)
2015 . The crystal structure of full-length E. coli YidC revealed that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans , which lacks a periplasmic domain. (tcdb.org)
The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
Widespread domain in signalling and nuclear proteins. (embl.de)
Taxonomic distribution of proteins containing SAM domain. (embl.de)
The complete taxonomic breakdown of all proteins with SAM domain is also avaliable . (embl.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing SAM domain in the selected taxonomic class. (embl.de)
This represents a new function for the SAM domain family, which is well characterized for mediating protein-protein interactions. (embl.de)
We show here that the SAM domain of Saccharomyces cerevisiae Vts1 binds RNA with the same specificity as Smaug and that Vts1 induces transcript degradation through a mechanism involving the cytoplasmic deadenylase CCR4. (embl.de)
His Ubiquitin ligase research integrates issues from DNA ligase and Transmembrane domain. (research.com)
T. J. Park, S. S. Choi, G. A. Gang, Y. Kim, High-Level Expression and Purification of the Second Transmembrane Domain of Wild-Type and Mutant Human Melanocortin-4 Receptor for Solid-State NMR Structural Studies, Protein Expression and Purification, Volume 62, (Issue 2), December 2008, Pages 139-145. (praiseworthyprize.org)

Some of these proteins exhibit 11 TMSs. (tcdb.org)

Nearly all mitochondrial proteins are encoded by the nuclear genome and imported into mitochondria after synthesis on cytosolic ribosomes. (escholarship.org)
These precursor proteins are translocated into mitochondria by the TOM complex, a protein-conducting channel in the mitochondrial outer membrane. (escholarship.org)
Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential transport systems for proper insertion of inner membrane and matrix proteins in mitochondria. (uni-goettingen.de)
The TOM and the TIM23 complexes cooperate for importing presequence-containing proteins into mitochondria. (uni-goettingen.de)
The ultra-modern look at through diagnosed a unique copper reductase enzyme, the histone H3-H4 tetramer, which binds to Cu2+ and catalyses its discount to Cu+ in Saccharomyces cerevisiae yeast to hold the characteristic of the electron delivery chain in mitochondria. (alliedacademies.org)

The structure contains 12 transmembrane helices, with the first 10 consisting of an inverted structural repeat of 5 transmembrane helices like LeuT (TC #2.A.22.4.2). (tcdb.org)

Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)

5] "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. (tcdb.org)
11] "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry. (tcdb.org)

2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)

The biogenesis of most proteins in the mitochondrial matrix and the inner membrane relies on the function of the TIM23 complex. (frontiersin.org)

After ligand binding, signal transduction is mediated by the interaction of activated Ste2p with its G protein (Gpa1p). (tennessee.edu)

The TIM22 complex is required for the import of polytopic inner membrane proteins which lack a presequence but have internal targeting signals. (uni-goettingen.de)
This result and other findings suggested that Mgr2p controls the lateral release of inner membrane proteins harboring a stop-transfer signal that follows an N-terminal amino acid signal. (frontiersin.org)

Distant homologues include the Lec15/Lec35 suppressor, SL15, of Chinese hamster ovary cells and ERS1, the ERD suppressor in S. cerevisiae. (wikipedia.org)
We found that one suppressor, the transmembrane protein PTR-23, is co-expressed with osm-8 in the hypodermis and strongly suppresses several Osm-8 phenotypes, including the transcriptional activation of many osmosensitive mRNAs, constitutive glycerol accumulation, and osmotic stress resistance. (prolekarniky.cz)

These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
The discount of Cu2+ to Cu+ is essential for its access into cells and is mediated through copper reductases, together with participants of the plasma membrane-sure six transmembrane epithelial antigen of the prostate (STEAP) family. (alliedacademies.org)

In its GTP-bound form, Cdc42 binds several effectors that help direct polarized cell growth: repolarizing actin and microtubules, directing polarized exocytosis via this reoriented cytoskeleton and by direct contact with exocytic machinery, and recruiting proteins such as septins that form a boundary restricting the region of cell growth. (rupress.org)

Mark Hochstrasser has researched Proteasome in several fields, including Degron, Functional studies, Protein quality and Protein folding. (research.com)

3] "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II. (tcdb.org)

The pheromone receptors have seven hydrophobic segments and are coupled to a heterotrimeric G protein. (berkeley.edu)

Along with mushrooms and molds, S. cerevisiae belongs to the Kingdom Fungi due to the presence of a cell wall made out of chitin, a polysaccharide polymer that's found not only in Fungi, but also in the exoskeletons of insects and crustaceans. (jove.com)

C-terminal PTS1 [(SAC)-(KRH)-(LM)] and N-terminal PTS2 [(RK)-(LIV)-X 5 -(HQ)-(LA)] targeting sequences are recognized by Pex5p and Pex7p, respectively, which may shuttle with the substrate protein to the peroxisomal lumen. (tcdb.org)

The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)
2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)

Using this protein expression platform, we have successfully prepared a variety of human transmembrane proteins, including a variety of GPCR family member proteins. (kmdbioscience.com)

These proteins are often homologous, and their similar sequences indicate that the organisms share a common ancestor. (jove.com)
Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
Mgr2p/Romo1 bridges TIM23 to the presequence translocase-associated motor (PAM) and is thought to regulate the lateral release of proteins harboring stop-transfer sequences. (frontiersin.org)
To circumvent these problems, we have developed a protocol for CNBr cleavage of protein fusions, which is particularly useful for Met-Ser sequences. (praiseworthyprize.org)

This basic two-step process has been confirmed in most mammalian cells, although its regulation and the repertoire of specific accessory proteins are likely to be highly context dependent. (centrodinoferrari.com)
They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
We use Gibco cell culture medium and related transfection reagents to ensure the high quality and high expression level of each batch of mammalian cell protein expression products. (kmdbioscience.com)

While direct structural efforts including x-ray crystallography, NMR and ab initio approaches have been effective tools in elucidating the structure of ZIPs, direct elucidation of the oligomeric state of these proteins is essential in understanding how wild type ZIP proteins function and whether mutations alter the oligomeric state of ZIPs. (bvsalud.org)
Although it's far much less considerable than different metals which include iron and zinc, copper is extensively applied as a catalytic or structural cofactor through enzymes and proteins which can be enormously applicable to cardiac body structure and pathology. (alliedacademies.org)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

This ligase attaches ubiquitin to both nuclear regulatory proteins and to misfolded membrane proteins degraded at the ER (ER-associated degradation or ERAD). (yale.edu)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)

Organic osmolytes accumulation is mediated by metabolic biosynthesis or transport into the cell via specialized organic osmolyte transporter proteins [5] . (prolekarniky.cz)

4] "Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases. (tcdb.org)

10] "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae. (tcdb.org)
We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)

Different classes of membrane proteins that range from 2-24 transmembrane domains have been successfully expressed. (premasbiotech.com)
There are 64647 SAM domains in 49933 proteins in SMART's nrdb database. (embl.de)
These proteins have predominantly been defined to contain either four or six transmembrane domains. (uni-goettingen.de)

This subfamily is composed of the MAPKs Sty1 from Schizosaccharomyces pombe, Hog1 from Saccharomyces cerevisiae, and similar proteins. (umbc.edu)

The most relevant proteins involved in the mitochondrial fusion process are three GTPase dynamin-like proteins: mitofusin 1 (MFN1) and 2 (MFN2), located in the outer mitochondrial membrane, and optic atrophy protein 1 (OPA1), in the inner membrane. (centrodinoferrari.com)

Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)

In cystinotic renal proximal tubules (RPTs), diminished cystinosin function appears to result in reduced reabsorption of solutes by other secondary transporters such as the Na+/Phosphate cotransporter, due to decreased expression of these other transport proteins. (wikipedia.org)

ZIP transmembrane proteins are expressed in the plasma membrane or membranes of intracellular organelles and have unique expression profiles across cell types. (bvsalud.org)
Given that mucins and transmembrane proteins play similar roles in yeast osmoregulation, our findings suggest a possible evolutionarily conserved role for the mucin-plasma membrane interface in eukaryotic osmoregulation. (prolekarniky.cz)

The coat on the budding vesicle comprises two layers, an inner layer of adaptor proteins (gray ovals) and an outer layer that forms a polyhedral cage. (biologists.com)
The specificity of vesicle trafficking: coat proteins and SNAREs. (kegg.jp)

Intact folded proteins and even oligomeric proteins can be transported. (tcdb.org)
Unfortunately, several tools to quantify oligomeric states of proteins require overexpression of proteins which can lead to artifacts in experimental results. (bvsalud.org)
In contrast, fluorescence correlation spectroscopy (FCS) is a single-molecule technique which can be used to quantify the oligomeric state of transmembrane proteins. (bvsalud.org)
This chapter describes how to implement FCS, focused on ZIP transporters, to quantify the oligomeric state of transmembrane in vivo. (bvsalud.org)
Taken together, FCS is a powerful mechanism to investigate the oligomeric state of proteins embedded within membranes of cells. (bvsalud.org)

Both of these suppressors, when overexpressed, have been reported to influence retention of lumenal endoplasmic reticular proteins as well as glycosylation in the Golgi apparatus. (wikipedia.org)
Despite the presence of this motif, Emp47p was shown to be a Golgi protein at steady-state. (rupress.org)
To investigate whether Emp47p undergoes retrograde transport from the Golgi to the ER like other di-lysine-tagged proteins we developed an assay to measure this step after block of forward transport in a sec12 mutant. (rupress.org)
Cdc42 interacts with proteins that regulate endocytosis and exocytosis and mediate traffic between ER and Golgi. (rupress.org)

Alterations in mitochondrial dynamics due to mutations in proteins involved in the fusion-fission machinery represent an important pathogenic mechanism of human diseases. (centrodinoferrari.com)

P39928) Osmolarity two-component system protein SLN1 (EC 2.7.3. (yeastrc.org)

Anatomy22

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Eukaryotes7

TOPOLOGY2

Gene3

Pathway8

Ubiquitin-like proteins2

Translocation5

Strain3

Recombinant4

Pore4

Peptides1

Homologous proteins2

Domain16

TMSs1

Mitochondria7

Helices1

Target proteins1

Mass spectrometry2

Lipid1

Biogenesis1

Signal transduction1

Inner membrane proteins2

Suppressor2

Antigen2

Binds1

Proteasome1

Fragment1

Segments1

Fungi1

Substrate protein1

Hydrophilic2

GPCR1

Sequences4

Mammalian3

Structural3

Nuclear2

Biosynthesis1

Uptake1

Phosphorylation2

Domains3

Schizosaccharomyces1

Involved in the mitochondrial1

Matrix proteins1

Transport proteins1

Plasma membrane2

Vesicle2

Oligomeric5

Golgi4

Mechanism1

SLN11