• Gene
  • Translocating chain-associated membrane protein 2 is a protein that in humans is encoded by the TRAM2 gene. (wikipedia.org)
  • What does this gene/protein do? (cancerindex.org)
  • Gene expression profiling of acute myeloid leukemia with translocation t(8;16)(p11;p13) and MYST3-CREBBP rearrangement reveals a distinctive signature with a specific pattern of HOX gene expression. (springer.com)
  • Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. (wikipedia.org)
  • EVI5L (Ecotropic Viral Integration Site 5-Like) is a protein that in humans is encoded by the EVI5L gene. (wikipedia.org)
  • Some of the main transcription factors predicted to bind to the promoter includes: activator-, mediator- and TBP-dependent core promoter element for RNA polymerase II transcription from TATA-less promoters, p53 tumor suppressor, brachyury gene, mesoderm developmental factor, EGR/nerve growth factor induced protein C & related factors, and GLI zinc finger family. (wikipedia.org)
  • Given this, the paralogs of the gene are associated with starvation-induced autophagosome formation and trafficking and translocation of GLUT4-containing vesicles. (wikipedia.org)
  • The expressed VSG gene is located downstream of 76‐bp repeats and the eight expression site‐associated genes (ESAGs). (els.net)
  • CCR4-NOT transcription complex subunit 7 is a protein that in humans is encoded by the CNOT7 gene. (wikipedia.org)
  • The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. (wikipedia.org)
  • In molecular biology, DiGeorge syndrome critical region gene 5 (non-protein coding), also known as DGCR5, is a long non-coding RNA. (wikipedia.org)
  • Endogenous Gene Tagging , Chapter in Methods in Molecular Biology Volume 1239, pp 231-240, 2015. (sigmaaldrich.com)
  • SKOV3 cells that have the genomic HER2 gene endogenously tagged with GFP gene and the genomic EGFR gene - with RFP gene. (sigmaaldrich.com)
  • A549 cells that have the genomic epidermal growth factor receptor (EGFR) gene endogenously tagged with GFP gene. (sigmaaldrich.com)
  • Protein Mdm4 is a protein that in humans is encoded by the MDM4 gene. (wikipedia.org)
  • The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. (wikipedia.org)
  • The cynS gene that encodes cyanase was identified among bacterial, fungal, and plant sequences in public databases, and the gene was particularly prevalent among cyanobacteria, including numerous Prochlorococcus and Synechococcus strains. (embl-heidelberg.de)
  • The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. (wikipedia.org)
  • This gene is expressed at high levels in a number of fetal epithelia. (wikipedia.org)
  • Zebrafish Fat1 was found to bind the protein scribble and regulate Hippo signalling Using the human SHSY5Y cell line as a model of neuronal differentiation, human FAT1 was shown to regulate Hippo kinase components with loss of FAT1 leading to nucleocytoplasmic relocation of TAZ and enhanced transcription of the Hippo target gene CTGF. (wikipedia.org)
  • Regulation of protein translation constitutes a crucial step in control of gene expression. (rsc.org)
  • The central dogma of molecular biology states that genetic information flows sequentially from DNA, via messenger RNA (mRNA), to proteins, the often final functional products of gene expression. (rsc.org)
  • Thanks to a variety of global transcriptome analysis methods such as DNA micro-array chip hybridization (micro-array) 2 and RNA-sequencing (RNA-seq), 3 much has been revealed regarding regulation of gene expression at the mRNA level. (rsc.org)
  • Kinesin-like protein KIF3B is a protein that in humans is encoded by the KIF3B gene. (wikipedia.org)
  • The protein encoded by this gene forms a heterotrimeric motor complex with kinesin family member 3A and KAP3 (kinesin accessory protein 3) to drive intra-flagellar transport and possibly to aid in chromosome movement during mitosis and meiosis. (wikipedia.org)
  • ETV6 (i.e. translocation-Ets-leukemia virus) protein is a transcription factor that in humans is encoded by the ETV6 (previously known as TEL) gene. (wikipedia.org)
  • However, ETV6 is also an anti-oncogene or tumor suppressor gene in that mutations in it that encode for a truncated and therefore inactive protein are also associated with certain types of cancers. (wikipedia.org)
  • the gene is expressed in virtually all cell types and tissues. (wikipedia.org)
  • Two unrelated kindreds were found to have autosomal dominant inherited mutations in the ETV6 gene, one family with a germline DNA substitution termed L349P that lead to replacing leucine with proline at amino acid 349 in the DNA binding domain of the ETV6, the second, termed N385fs, in germline DNA caused the lose of five base pairs ETV6 and a truncated ETV6 protein. (wikipedia.org)
  • The protein encoded by this gene is one of three V1 domain G subunit proteins. (wikipedia.org)
  • 1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). (wikipedia.org)
  • Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. (wikipedia.org)
  • The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g. (wikipedia.org)
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a protein that in humans is encoded by the SMARCD1 gene. (wikipedia.org)
  • The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. (wikipedia.org)
  • Arginine-glutamic acid dipeptide repeats protein is a protein that in humans is encoded by the RERE gene. (wikipedia.org)
  • This gene encodes a member of the atrophin family of arginine-glutamic acid (RE) dipeptide repeat-containing proteins. (wikipedia.org)
  • Lipoma HMGIC fusion partner-like 1 protein is a protein that in humans is encoded by the LHFPL1 gene. (wikipedia.org)
  • This gene is a member of the lipoma HMGIC fusion partner (LHFP) gene family, which is a subset of the superfamily of tetraspan transmembrane protein encoding genes. (wikipedia.org)
  • Mutations in one LHFP-like gene result in deafness in humans and mice, and a second LHFP-like gene is fused to a high-mobility group gene in a translocation-associated lipoma. (wikipedia.org)
  • Voltage-dependent anion-selective channel protein 2 is a protein that in humans is encoded by the VDAC2 gene on chromosome 10. (wikipedia.org)
  • regulates
  • Among these are a novel G-protein coupled receptor that instructs Schwann cells to make myelin in peripheral nerves, receptors that control migration of glial cells along growing axons, a kinesin motor protein that is essential for mRNA localization and normal membrane compaction in myelinating oligodendrocytes, and a transcription factor that regulates the migration of the cells that form myelin in the brain and spinal cord. (stanford.edu)
  • The ETV6 protein regulates the development and growth of diverse cell types, particularly those of hematological tissues. (wikipedia.org)
  • binds
  • Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (wikipedia.org)
  • TrpC4 exists as two splice variants (TrpC4 α and TrpC4 β ) that differ only in an 84-residue sequence that binds to phosphatidylinositol(4,5) bis phosphate (PIP 2 ), the substrate of PLC β 1b. (aspetjournals.org)
  • We conclude that TrpC4 α is selectively involved in mechanisms downstream of PLC β 1b culminating in cardiomyocyte hypertrophy, and that the hypertrophic response is dependent on the TrpC4 α splice variant-specific sequence that binds to PIP 2 . (aspetjournals.org)
  • Omomyc selectively targets Myc protein interactions: it binds c- and N-Myc, Max and Miz-1, but does not bind Mad or select HLH proteins. (pubmedcentralcanada.ca)
  • It contains an N-terminal SH3 domain, which in Dock180 binds ELMO (a family of adaptor proteins which mediate recruitment and efficient GEF activity of Dock180), and a C-terminal proline-rich region which, in Dock180, binds the adaptor protein CRK. (wikipedia.org)
  • kinase
  • Axonal R-Ras GAP activity downregulates phosphatidylinositol 3-kinase signaling pathway, and thereby induces inactivation of a microtubule assembly promoter protein, CRMP-2. (jneurosci.org)
  • EVI5L has been shown to interact with NUDT18 (nucleoside diphosphate linked moiety X)-type motif 18 and SRPK2 (serine/threonine-protein kinase 2). (wikipedia.org)
  • on target proteins such as Raf to control mitogen-activated protein kinase activation, Cdc25 to control G2/M cell cycle progression, and BAD and FKHRL1 to control apoptosis. (rupress.org)
  • TrpC3, -6, and -7 are activated directly by DAG as well as indirectly following DAG-induced protein kinase C activation ( Hardie, 2003 ). (aspetjournals.org)
  • In contrast, TrpC1, -4, and -5 do not respond to DAG, although TrpC1 can be activated by protein kinase C, and thus indirectly by DAG. (aspetjournals.org)
  • Sphingosine kinase 1 phosphorylates sphingosine to sphingosine-1-phosphate (S1P) SK1 is normally a cytosolic protein but is recruited to membranes rich in phosphatidate (PA), a product of Phospholipase D (PLD) Sphingosine-1-phosphate (S1P) is a novel lipid messenger with both intracellular and extracellular functions. (wikipedia.org)
  • nucleus
  • EVI5L is a soluble protein that localizes in the nucleus. (wikipedia.org)
  • These results strongly suggest that the molecular mechanism of the nuclear import of SXR was different from that of another xenosensor, the constitutively active receptor, whose translocation into the nucleus is mediated by a leucine-rich xenochemical response signal in its ligand binding domain. (aspetjournals.org)
  • The encoded protein co-localizes with a transcription factor in the nucleus, and its overexpression triggers apoptosis. (wikipedia.org)
  • bind
  • However, physiological interaction between Lpd and Ras family GTPases still remains unclear, and the identity of which Ras family proteins directly bind to Lpd and function upstream of Lpd in neurons has been rarely understood. (jneurosci.org)
  • Recombinant proteins containing the SBP-Tag bind to streptavidin and this property may be utilized in specific purification, detection or immobilization strategies. (wikipedia.org)
  • The SBP-Tag has been shown to bind streptavidin with an equilibrium dissociation constant of 2.5nM and is readily eluted with biotin under native conditions. (wikipedia.org)
  • There are several relatively abundant mammalian proteins that inherently associate with the IMAC matrices that bind to the more commonly used Polyhistidine-tag (His-tag). (wikipedia.org)
  • The SBP-Tag will also bind to streptavidin or streptavidin reagents in solution. (wikipedia.org)
  • Proteomics
  • This trend is now beginning to shift thanks to recent advances in next-generation sequencing, proteomics, and microscopy based methodologies which allow accurate monitoring of protein translation rates, from single target messenger RNA molecules to genome-wide scale studies. (rsc.org)
  • Ironically, multitude of studies using quantitative proteomics in conjugation with transcriptomics have highlighted that globally little correlation exists between mRNA and protein levels in various biological systems, 6-11 although individual protein/mRNA ratio levels seem to be conserved. (rsc.org)
  • mRNA
  • The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. (wikipedia.org)
  • 1 As a result of decades of intense research in both prokaryotes and eukaryotes, it is mechanistically well understood how DNA is transcribed and processed into mRNA, and how mRNA is translated into proteins. (rsc.org)
  • VASP
  • Lamellipodin (Lpd) has been identified as an Ena/VASP family protein-interacting molecule harboring a Ras association (RA) domain, pleckstrin-homology (PH) domain, and proline-rich region. (jneurosci.org)
  • membrane protein
  • Applications of these engineered associations include the visualization of specific proteins within living cells, monitoring of the kinetics of the translation of individual proteins in an in vitro translation system, control of the integration of a multi-spanning membrane protein into the endoplasmic reticulum by fusing the SBP-Tag to the N-terminal translocation sequence and then halting integration with streptavidin and restarting integration with biotin. (wikipedia.org)
  • expression
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • 14 Work horse technology: Micro.arrays Epigenetic studies CGH Transcriptional profiling Genotyping Methylation Protein Expression Splice Variant studies Protein selection or attachment by aptamers Protein-ssDNA interactions Protein-dsDNA interactions Apart from quality issues, data interpretation is currently the main bottleneck in microarray analyses. (docplayer.es)
  • Expression data from expressed sequence tag mapping, microarray and in situ hybridization shows EVI5L has ubiquitously low expression. (wikipedia.org)
  • Some human pathogens use programmed DNA rearrangements to evade the immune system by varying the expression of their antigenic surface proteins. (els.net)
  • Human pathogens use programmed DNA rearrangements to vary expression of antigenic surface proteins to avoid the host immune system. (els.net)
  • Heightened PLC β 1b expression caused TrpC4 α , but not TrpC4 β , translocation to the sarcolemma, where it colocalized with PLC β 1b. (aspetjournals.org)
  • The FAT1 protein once translated undergoes furin mediated S1 cleavage forming a non-covalent heterodimer before achieving cell surface expression although this processing is often perturbed in cancer cells which express non-cleaved FAT1 on the cell surface. (wikipedia.org)
  • Protein Expression and Purification. (wikipedia.org)
  • nucleotide
  • 10 GenBank contains nucleotide sequences for more than named organisms GenBank. (docplayer.es)
  • All of these proteins are in the guanosine nucleotide-binding protein family There are 63 orthologs of EVI5L that have been identified including mammals, birds, reptiles, and fish. (wikipedia.org)
  • It is a member of the DOCK-B subfamily of the DOCK family of guanine nucleotide exchange factors (GEFs) which function as activators of small G proteins. (wikipedia.org)
  • GEFs activate G proteins by promoting this nucleotide exchange. (wikipedia.org)
  • transcription
  • In addition, Chromatin Immunoprecipitation (ChIP) followed by micro-array chip hybridization (ChIP-chip) 4 or next-generation sequencing (ChIP-seq) 5 methods have enabled analysis of transcription factor-DNA associations on genome-wide scales. (rsc.org)
  • intracellular
  • In addition, the encoded protein may be involved in the intracellular movement of membranous organelles. (wikipedia.org)
  • V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. (wikipedia.org)
  • Dock3 (Dedicator of cytokinesis 3), also known as MOCA (modifier of cell adhesion) and PBP (presenilin-binding protein), is a large (~180 kDa) protein involved in intracellular signalling networks. (wikipedia.org)
  • receptor
  • We recently reported that the semaphorin 4D (Sema4D) receptor Plexin-B1 induces repulsion in axon and dendrites by functioning as a GTPase-activating protein (GAP) for R-Ras and M-Ras, respectively. (jneurosci.org)
  • similarity
  • The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. (wikipedia.org)
  • Dock3 exhibits the same domain arrangement as Dock180 (a member of the DOCK-A subfamily and the archetypal member of the DOCK family) and these proteins share a considerable (40%) degree of sequence similarity. (wikipedia.org)
  • leucine-rich
  • identified a leucine-rich sequence within the COOH-terminal helix (helix αI) of 14-3-3 that was highly homologous to known nuclear export signals (NESs). (rupress.org)
  • These authors showed that point mutations in this leucine-rich sequence of 14-3-3 prevented Cdc25 from being sequestered in the cytoplasm by disrupting its binding to 14-3-3. (rupress.org)
  • actin
  • Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate actin-filament dynamics. (wikipedia.org)
  • Lpd was expressed in F-actin-rich distal dendritic processes and was required for both basal and M-Ras-mediated dendrite development. (jneurosci.org)
  • The following loci were tagged: LMNB1 (lamin B1, nuclear envelope), TUBA1B (α-tubulin 1b, microtubules), ACTB (β-actin, actin stress fibers) by BFP, GFP and RFP respectively in the same cells (U2OS). (sigmaaldrich.com)
  • U2OS cells with a cytoskeletal protein β-actin GFP tagged. (sigmaaldrich.com)
  • interaction
  • citation needed] The SBP-Tag has been used to reversibly immobilize recombinant proteins onto streptavidin-functionalized surfaces thereby permitting interaction assessment such as by surface plasmon resonance (SPR) techniques with re-use of the functionalized surface. (wikipedia.org)
  • Phylogenetic
  • Phylogenetic analysis of cynS sequences retrieved from the Global Ocean Survey database identified >60% as belonging to unicellular marine cyanobacteria, suggesting an important role for cyanase in their nitrogen metabolism. (embl-heidelberg.de)
  • Phylogenetic analysis of the sequences suggests that Van OMT-2 and Van OMT-3 evolved from the V. planifolia COMT. (deepdyve.com)
  • family
  • Transforming growth factor‐β1 (TGF‐β1) is the prototype of a family of structurally related cytokines, which also includes activins and bone morphogenetic proteins (BMPs). (embopress.org)
  • Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. (wikipedia.org)
  • guanosine
  • In their resting state G proteins are bound to Guanosine diphosphate (GDP) and their activation requires the dissociation of GDP and binding of guanosine triphosphate (GTP). (wikipedia.org)
  • Splice Variant
  • PLC β 1b differs from the other splice variant PLC β 1a by only a 32-residue C-terminal sequence, and the splice variant specificity allowed us to identify factors critically involved in Gq/PLC β 1b-mediated responses. (aspetjournals.org)
  • hippocampus
  • He found that postsynaptic proteins were degraded in the hippocampus by polyubiquitination after the retrieval of contextual fear memory. (wikipedia.org)
  • Dock3 is specifically expressed in neurones (primarily in the cerebral cortex and hippocampus). (wikipedia.org)
  • histone
  • The monocytic leukemia zinc finger protein MOZ is a histone acetyltransferase. (springer.com)
  • A similar protein in mouse associates with histone deacetylase and is thought to function as a transcriptional co-repressor during embryonic development. (wikipedia.org)
  • Cell
  • We also discuss how these methods can be utilized to spatially resolve the sites of protein translation inside the cell, and implication of such spatial information on our understanding of the role and significance of localized translation in cell biology. (rsc.org)
  • interactions
  • Taken together, these studies raise the possibility that the primary function of helix αI may be to mediate interactions between 14-3-3 and many phosphoprotein ligands but do not prove that this sequence cannot function as an NES. (rupress.org)
  • This molecule - instead - appears to cause an edge-specific perturbation that destroys some protein interactions of the Myc node and keeps others intact, with the result of reshaping the Myc transcriptome. (pubmedcentralcanada.ca)
  • Myc operates within a highly interconnected interactome network and a possible strategy for targeting Myc oncogenic function is dominant interference of Myc protein interactions. (pubmedcentralcanada.ca)
  • cellular
  • Crucially, with the advent of methods that allow measurement of protein translation rates on a global scale, it has become apparent that translational control seems to be the defining step in determining the steady-state levels of most cellular proteins. (rsc.org)
  • Dock3 is part of a large class of proteins (GEFs) which contribute to cellular signalling events by activating small G proteins. (wikipedia.org)
  • specifically
  • Cardiomyocyte hypertrophy downstream of Gq-coupled receptors is mediated specifically by PLC β 1b that is scaffolded onto a SH3 and ankyrin repeat protein 3 (Shank3) complex at the sarcolemma. (aspetjournals.org)
  • Dock3 specifically activates the small G protein Rac. (wikipedia.org)