I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
8. Melchers, F.: Biosynthesis, transport and secretion of immunoglobulin in plasma cells. (uni-mainz.de)
High spatial-resolution MSI will elucidate the compartmentation of oil assembly in developing seeds, which is essential for the redesign of lipid biosynthesis in seeds in order to improve yield. (ukri.org)
For instance, the transformation of plants with a yeast-derived invertase targeted to different subcellular compartments has led to the elucidation of several key aspects of sugar metabolism, including phloem loading mechanisms, the regulation of photosynthesis by sugars, the importance of sugar-metabolism compartmentation with regards to sucrose biosynthesis, storage and distribution, as well as the role of cell-wall invertase in phloem unloading and sink strength. (sun.ac.za)
Protein-protein inte1
9. Melchers, F., Köhler, G., Messer, W.: Stabilization of conformations of Escherichia coli beta-galactosidase by specific antibodies.In: 23rd Mossbach Colloquium of the Germany Society for Biological Chemistry on Protein-Protein Interactions, pp. 409-425, Springer, Berlin, 1972. (uni-mainz.de)
Phenomena1
In: 'Molecular Aspects of Membrane Phenomena,' eds. (uni-mainz.de)
Superoxide1
MitoQ consequently decreased the mitochondrial membrane potential and mitochondrial superoxide production by these cells. (biomed.news)
Biology1
In: 'Perspectives in Membrane Biology,' eds. (uni-mainz.de)
Synthesis3
11. Melchers, F.: Synthesis, transport and secretion of immunoglobulin in lymphoid cells. (uni-mainz.de)
17. Melchers, F., Andersson, J.: Immunoglobulin production in B-lymphocytes: synthesis of the membrane-bound receptor and the secreted serum glycoprotein immunoglobulin M. Biochem. (uni-mainz.de)
The transcriptome response of A. caldus to elevated NaCl concentrations included changes in carbon flux, elevated glutathione synthesis, alterations in cell wall and membrane composition, the down-regulation in genes involved in flagellar synthesis and rotation, the reduced energy generation through sulfur oxidation, and the up-regulation in genes involved in DNA and protein repair. (bvsalud.org)
Mice2
Cyclic AMP compartmentation due to increased cAMP-phosphodiesterase activity in transgenic mice with a cardiac-directed expression of the human adenylyl cyclase type 8 (AC8)" (PDF). (wikipedia.org)
It reduced by ~50% the metastatic homing of human MIA PaCa-2 cells in the lungs of mice. (biomed.news)
Metabolism2
The genetic transformation of plants and the subsequent development of transgenic lines with disturbed sugar metabolism have made an unprecedented impact on the study of sugar translocation and -partitioning. (sun.ac.za)
Moreover, cell population measurements of mitochondrial and glycolytic metabolism using a Seahorse analyzer demonstrated that WT KRAS TP53-silenced cells display an increase of the basal respiration and tightly-coupled mitochondria, in the presence of glucose as substrate, compared to TP53 competent cells. (biomed.news)
Genes3
In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
Somatic mutations in genes involved in oncogenic signaling pathways, including KRAS and TP53, rewire the metabolic machinery in cancer cells. (biomed.news)
Specifically, we explored how somatic mutations in these genes and substrate availability (lactate, glucose, substrate deprivation) from the extracellular microenvironment affect bioenergetic parameters, including cellular ATP, NADH and mitochondrial membrane potential dynamics. (biomed.news)
Alterations1
On the other hand, oncogenic KRAS in TP53-deficient cells reversed the alterations in cellular ATP levels. (biomed.news)
Proliferation2
In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
Growth differentiation factor 9 (GDF9) stimulates proliferation and inhibits steroidogenesis by bovine theca cells: influence of follicle size on responses to GDF9. (ouhsc.edu)
Plant3
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
In this study, a similar strategy of transgenic plant analysis was employed to expand our insight into the regulation of sugar partitioning. (sun.ac.za)
Plants5
Application of Si to plants promotes cell walls strength and provides additional support through increased mechanical and biochemical support. (researchsquare.com)
The invention furthermore relates to novel nucleic acid sequences which code for the Δ6-desaturases used in the method, gene constructs comprising these nucleic acid sequences, a vector and transgenic plants comprising at least one nucleic acid sequence or a gene construct. (rothamsted.ac.uk)
Lipids are key components of biological system ranging from plants to mammals. (ukri.org)
The yeast-invertase Suc2 gene, from Saccharomyces cere visiae , was overexpressed in either the cytosol, vacuole or apoplast of transgenic tobacco plants. (sun.ac.za)
Transgenic lines overproducing the yeast-derived invertase in either the vacuole (Vac-Inv) or apoplast (Apo-Inv) were utilised to analyse the effect of the altered sugar levels in sink and source organs on the expression of sugar transporters, as well as the endogenous cell wall invertase and inhibitors in these plants. (sun.ac.za)
Human2
Knockdown of MBP-1 in human prostate cancer cells delays cell cycle progression. (ouhsc.edu)
We here set out to determine, at the single cell level, metabolic signatures in human colon cancer cells engineered to express combinations of activating KRAS gene mutations and TP53 gene deletions. (biomed.news)
Expression4
Expression and effect of fibroblast growth factor 9 in bovine theca cells. (ouhsc.edu)
Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy. (ouhsc.edu)
Using four different cancer cell lines, we report that, at clinically relevant concentrations (100-500 nM), MitoQ selectively repressed mesenchymal pancreatic cancer cell respiration, which involved the inhibition of the expression of PGC-1α, NRF1 and a reduced expression of electron-transfer-chain complexes I to III. (biomed.news)
Phenotypically, MitoQ further inhibited pancreatic cancer cell migration, invasion, clonogenicity and the expression of stem cell markers. (biomed.news)
Organisms1
The powerful quantitative metabolomics-fluxomics approach will help our ability to engineer unicellular and multicellular organisms evolve from trial-and-error to a more predictable process, and from cells to organ and organisms. (mdpi.com)
Molecular1
Cell) books are molecular residing phrases and are useful from divide highlights under house. (cutechabeads.com)
Walls1
In the roots of Steen, White French, Jacquez and 1202 C the formation of multinucleate syncytia by the destruction of the walls of groups of cells often occurred. (sun.ac.za)
Distribution2
The compartmentation and distribution of metabolites between mitochondria and the rest of the cell is a key parameter of cell signalling and pathology. (biomed.news)
Using this procedure revealed the in vivo compartmentation of mitochondrial metabolites and will enable assessment of the distribution of metabolites between the cytosol and mitochondria during a range of situations in vivo. (biomed.news)
Function1
20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
Analysis1
This approach extends the analysis of complex systems from description to prediction, including control of complex dynamic behavior ranging from biological rhythms to catastrophic lethal arrhythmias. (mdpi.com)
Single cell level1
The AP-MALDI source offers high spatial-resolution (5 - 10 microm), at the single cell level, while the ESI interface is compatible with other imaging modalities e.g. (ukri.org)
Role1
Despite this abundance, Al is not considered an essential element and so far no experimental evidence has been put forward for a biological role. (frontiersin.org)
Volume1
The quantity of volume or surface area of CELLS. (ouhsc.edu)
Production1
Intriguingly, cytosolic, mitochondrial and overall cellular ATP measurements revealed that, in WT KRAS cells, TP53 deficiency leads to an enhanced ATP production in the presence of extracellular lactate and glucose, and to the greatest increase in ATP following a starvation period. (biomed.news)
Groups1
12. Melchers, F.: Possible roles of the carbohydrate groups in biological functions of the glycoproteins, immunoglobulin M and G. In: 'Int. Symp. (uni-mainz.de)
Total1
This graph shows the total number of publications written about "Cell Size" by people in this website by year, and whether "Cell Size" was a major or minor topic of these publications. (ouhsc.edu)
Journal1
The Journal of Biological Chemistry. (wikipedia.org)