Transgenic promoter regions genetic. Medical search. Frequent questions

We have developed a simple and sensitive method for conditional cell ablation in transgenic mice, called "toxin receptor-mediated cell knockout. (nih.gov)
We expressed the diphtheria toxin (DT) receptor in transgenic mice using a hepatocyte-specific promoter and found that injection of DT caused fulminant hepatitis. (nih.gov)
The promoter of the GABA(A) receptor δ subunit gene was analyzed in transgenic mice and in cultured cells to study sequences involved in neuronal subtype-specific gene expression. (psu.edu)
RePub, Erasmus University Repository: Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice. (eur.nl)
A dectin-2 lentivector encoding the human melanoma antigen NY-ESO-1 primed an NY-ESO-1-specific CD8(+) T-cell response in HLA-A2 transgenic mice and stimulated a CD4(+) T-cell response to a newly identified NY-ESO-1 epitope presented by H2 I-A(b). (ox.ac.uk)
In this study, we identified a transcriptional enhancer from the HRC gene that is sufficient to direct the expression of lacZ in the expression pattern of endogenous HRC in transgenic mice. (ox.ac.uk)
Mice with the required genotypes were obtained by crossing transgenic, mdm2 +/− , and p53 −/− mice. (molvis.org)
In a wild-type genetic background ( mdm2 +/+ ), lens damage and microphthalmia were observed only in mice homozygous for Tg p53 ( t/t ). (molvis.org)
Transgenic mice that were created that had a mutation in the gene which codes for the transcription factor (NGFIB). (freeonlineresearchpapers.com)
First transgenic experiments were carried out on mice. (freeonlineresearchpapers.com)
Reproducible multi-stage progression to invasive squamous carcinoma of the epidermis has been achieved in transgenic mice expressing the HPV16 early-region genes, including the E6/E7 oncogenes, under the control of the human keratin-14 promoter/enhancer. (elsevierpure.com)
Although 100% of K14-HPV16 transgenic animals develop hyperplastic and/or dysplastic lesions in several inbred backgrounds, including C57BL/6, BALB/c, and SSIN/SENCAR, only mice backcrossed into the FVB/n background progress to malignant squamous cell carcinomas of two pathological grades, well differentiated and moderate/poorly differentiated (WDSC or MPDSC, respectively), each displaying characteristic patterns of malignant behavior. (elsevierpure.com)
The transition to the malignant state, in 21% of FVB/n transgenic mice, is characterized by alteration of the repertoire of keratin intermediate filament proteins expressed within neoplastic epidermis, such that WDSCs maintain expression of keratins common to terminally differentiating stratified keratinocytes (K10), whereas MPDSCs are distinguished from WDSCs by activation of embryonic and mucosal keratins (K13, K8, and K19). (elsevierpure.com)
When reporter mice were bred onto a genetic background expressing a lower affinity form of the Ah receptor (DBA allele), transgene and murine Cyp1a1 protein expression were both attenuated in the adult mouse liver upon xenobiotic challenge. (ox.ac.uk)
Here, we characterize Tpcn1 expression in two transgenic mouse lines (Tpcn1(XG716) and Tpcn1(T159)) and show expression of a novel evolutionarily conserved Tpcn1B transcript from an alternative promoter, raising important questions regarding the status of Tpcn1 expression in mice recently described to be Tpcn1 knockouts. (ox.ac.uk)
To study the function of DREAM in the brain, we used transgenic mice expressing a Ca(2+)-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). (ox.ac.uk)
The highest degrees of colocalization were observed in mice harbouring the 20-kb construct, suggesting a large promoter fragment is required to faithfully direct transgene expression in a 5-HT(1A) receptor like pattern. (ox.ac.uk)
To identify genetic elements that direct appropriate prenatal and postnatal expression of the ADA gene, a segment of DNA including the ADA promoter and 6.4 kilobases of the adjacent 5' flanking region was tested for the ability to direct the expression of a reporter gene in transgenic mice. (utmb.edu)
In seven lines of transgenic mice studied, this construct directed high levels of reporter gene expression in the placenta and forestomach and exhibited correct developmental regulation in these tissues. (utmb.edu)
MORp constructs designed from promoter regions upstream of the mouse Oprm1 gene (mMORp) were validated for transduction efficiency and selectivity in endogenous MOR+ neurons in the brain, spinal cord, and periphery of mice, with additional studies revealing robust expression in rats, shrews, and human induced pluripotent stem cell (iPSC)-derived nociceptors. (bvsalud.org)
In contrast to wild-type mice, the transgenic UMOD mice had marked improvement in blood pressure with furosemide (an inhibitor of NKCC2). (medscape.com)
Although HLA-B51 transgenic mice do not develop any manifestations of Behçet disease, their neutrophils show excessive function. (medscape.com)

We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. (nih.gov)
Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. (nih.gov)
However, promoters of these genes were demethylated and activated during retinal development. (nih.gov)
2) highly-methylated promoters of photoreceptor-related genes. (nih.gov)
This can be done through genetic engineering, which involves the insertion of specific genes or DNA sequences into an organism's genome. (tutorialspoint.com)
Reporter genes can be fused to the regulatory regions of other genes, allowing the expression of the target gene to be monitored in real-time. (tutorialspoint.com)
Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. (biomedcentral.com)
Use of these fruit specific/preferential promoters for genetic engineering can help with precise expression of beneficial genes and help with accurate prediction of the activity of new genes in host fruit plants. (biomedcentral.com)
Furthermore, a genome-wide search identified several genes that carry the Lmx1b-FoxC motif in their promoter regions. (ox.ac.uk)
Further studies showed that the J protein bound to the promoter of the E1 gene (a legume-specific flowering repressor) to downregulate E1 expression, thereby relieving the suppression of E1 on soybean FLOWERING LOCUS T ( FT ) genes, which leads to early flowering under short photoperiod. (chinbullbotany.com)
Moreover, we identified that PARIS actively modulates expression of PPARγ target genes by physically binding to the promoter regions. (nature.com)
In recent years, our understanding of genetic predisposition to PD has advanced vastly, including causative mutations in the genes encoding α-synuclein, LRRK2, parkin, PINK1, and DJ-1 just to name a few 5 . (nature.com)
The initiation of transcription and modulation of gene expression in eukaryotic genes is directed by a variety of DNA sequence elements collectively arranged in a larger sequence called promoter. (allindianpatents.com)
The minimal promoter or core promoter is a minimal stretch of contiguous DNA sequence that is sufficient to direct accurate initiation of transcription by RNA [Pol II machinery, Smale, S. T, genes dev 15, 2503-2508 2001]. (allindianpatents.com)
No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T 1 plants. (elsevierpure.com)
These transcription factors bind promoters and distant regulatory regions, including E-box elements, of genes whose expression is restricted to muscle cells. (unipv.it)
While these transgenic assays have been important in defining the structure of the cis -regulatory elements and identifying trans -acting factors that bind to them, their functions within the context of the endogenous genes remain poorly understood. (nih.gov)
The transcriptional silencers are clustered in the two regions whose interactions are required for the maintenance of silencing in the endogenous genes. (nih.gov)

Within this conserved region is a consensus site for myocyte enhancer factor 2 (MEF2) proteins that we show is bound efficiently by MEF2 and is required for transgene expression in all three muscle lineages in vivo. (ox.ac.uk)
Genetic analysis involves the study of an organism's genetic material, including its DNA, RNA, and proteins. (tutorialspoint.com)
These include unintended effects on the environment, such as the spread of transgenes to wild populations, and unintended effects on human health, such as allergic reactions to transgenic proteins. (tutorialspoint.com)
Here we survey advancement of a transgenesis way for genome, including genome-wide mapping of transcription begin regions, and present its tool by producing multiple steady transgenic lines expressing fluorescent proteins under many tissue-specific promoters. (careersfromscience.org)
The activation of the core promoter is done by the additional sequence of regulatory DNA sequence elements to which various proteins bind and subsequently interact with the transcription initiation complex to activate gene expression. (allindianpatents.com)
Identification of Potato Nuclear Proteins Binding to the Distal Promoter Region of the Proteinase Inhibitor-Ii Gene. (mpg.de)
Better promoters, enhancers, and other genetic elements have contributed to increased upstream production of proteins. (genengnews.com)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)

In conclusion, the δ subunit gene promoter/upstream region contains the information for neuronal subtype-specific expression in the entire brain except in the cerebellum and is selectively active in primary cortical neurons in vitro. (psu.edu)
Here, we report the isolation and analysis of 5' flanking genomic DNA regions upstream to the CAD gene, from two conifers, i.e. white spruce (Picea glauca (Moench) Voss) and loblolly pine (Pinus taeda L.). Sequence comparisons with available CAD gene promoters from angiosperms highlighted the conservation of cis-elements matching MYB, WRKY and bHLH binding sites. (ox.ac.uk)
Using established transgenic mouse lines where 8.5 kb of the rat CYP1A1 promoter is cloned upstream of the lacZ reporter gene (1), we describe the expression of the CYP1A1-driven reporter gene in all tissues through-out stages E7-E14 of embryonic development. (ox.ac.uk)
To further analyse the promoter of this receptor gene, additional upstream sequence information extending to -5.5 kb of the murine htr1a was generated and promoter fragments extending to -20 kb were analysed for activity in cell culture and transgenic animals. (ox.ac.uk)
These short regulatory elements are located at a varying distance from transcription start point, some regulatory elements (called proximal elements) are adjacent to core promoter while other elements can be positioned several kilobases upstream or downstream of the promoter (enhancers). (allindianpatents.com)
The chromosomal aberrations identified an imaginal leg enhancer about 35 kb upstream of the Scr promoter. (nih.gov)

This problem has been circumvented with the use of several knockout, knock-in, and overexpressing transgenic mouse models. (molvis.org)

Three independently established transgenic lines demonstrated a good correlation between the sensitivity of hepatocytes to DT and the expression level of the DT receptors. (nih.gov)

Regulation of gene expression involves post-translational modifications to histones which cause chromatin decondensation and recruit transcription factors at the promoter to initiate transcription. (utexas.edu)
The core promoter provides the site of action to RNA polymerase II which is a multisubunit enzyme with the basal or general transcription factors like, TFIIA, B, D, E, F and H. These factors assemble into a transcription pre initiation complex (PIC) that catalyzes the synthesis of RNA from DNA template. (allindianpatents.com)
Park et al analyzed TNF-alpha haplotypes in the promoter response element that affect the binding affinity of certain transcription factors. (medscape.com)

However, available tools and transgenic models for gaining long-term genetic access to MOR+ neural cell types and circuits involved in modulating pain, analgesia and addiction across species are limited. (bvsalud.org)
We investigate the role of Zeb2 in skeletal muscle differentiation using genetic tools and transgenic mouse embryonic stem cells, together with single-cell RNA-sequencing and in vivo muscle engraftment capability. (unipv.it)

Expression of a Yeast-Derived Invertase in the Cell-Wall of Tobacco and Arabidopsis Plants Leads to Accumulation of Carbohydrate and Inhibition of Photosynthesis and Strongly Influences Growth and Phenotype of Transgenic Tobacco Plants. (mpg.de)

Transfection studies using primary cortical neurons and astroglial cells revealed that the δ subunit gene promoter was selectively active in neurons even when truncated to a 267-bp core fragment. (psu.edu)
Together, our MORp toolkit provides researchers cell type specific genetic access to target and functionally manipulate mu-opioidergic neurons across a range of vertebrate species and translational models for pain, addiction, and neuropsychiatric disorders. (bvsalud.org)
We employed conditional translating ribosome affinity purification (TRAP) followed by RNA sequencing (TRAP-seq) for transcriptome profiling of DA neurons in transgenic Drosophila lines expressing human PARIS wild type (WT) or mutant (C571A). (nature.com)

Transgenic analysis of sugar beet xyloglucan endo-transglucosylase/hydrolase Bv-XTH1 and Bv-XTH2 promoters reveals overlapping tissue-specific and wound-inducible expression profiles. (herts.ac.uk)
Dive into the research topics of 'Transgenic analysis of sugar beet xyloglucan endo-transglucosylase/hydrolase Bv-XTH1 and Bv-XTH2 promoters reveals overlapping tissue-specific and wound-inducible expression profiles. (herts.ac.uk)
The use of tissue-specific promoters is critical for producing transgenic crops with improved economically important tissues, such as the fruits in citrus. (biomedcentral.com)
Here we present a method for transgenesis in using microinjection of DNA into single-cell stage embryos and demonstrate its robustness by generating multiple transgenic tissue-specific reporter lines. (careersfromscience.org)
This approach depends on the availability of tissue-specific promoters, and it does not allow further spatial selectivity within the promoter-defined region(s). (zfin.org)

As immunization with the optimal dose of the dectin-2 lentivector was similar to that stimulated by a lentivector containing a strong constitutive viral promoter, targeting antigen expression to dendritic cells can provide a safe and effective vaccine. (ox.ac.uk)
P-ubiZM1 is a constitutive promoter in corn (ie. (cbd.int)
In contrast to the absence of constitutive Cyp1a1 and lacZ transgene expression in tissues of the adult mouse, a constitutive cell-specific and time-dependent pattern of CYP1A1 promoter activity was observed in the embryo. (ox.ac.uk)
By comparison, constitutive CYP1A1 promoter activity in the embryo was identical in the presence of either the high or low affinity Ah receptor. (ox.ac.uk)
This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP. (elsevierpure.com)

To improve safety while retaining efficacy, we constructed a lentivector in which transgene expression was restricted to antigen-presenting cells using the mouse dectin-2 gene promoter. (ox.ac.uk)
In transgenic organisms, genetic analysis is used to determine the presence and expression of the foreign DNA sequences that have been introduced into the genome. (tutorialspoint.com)
Molecular analysis involves the study of the molecular mechanisms underlying gene expression and regulation in transgenic organisms. (tutorialspoint.com)
This includes the study of the promoter regions that regulate gene expression, the splicing mechanisms that generate different mRNA isoforms, and the translation mechanisms that produce the final protein products. (tutorialspoint.com)
For example, a promoter region can be fused to a gene encoding a fluorescent protein, and the expression of the protein can be visualized using fluorescence microscopy. (tutorialspoint.com)
Histochemical observations of different vegetative organs of the transgenic trees showed that this sequence was sufficient to drive GUS expression in lignifying tissues, and more specifically in differentiating xylem cells. (ox.ac.uk)
In addition, GUS expression driven by the PgCAD promoter was wound-inducible which was consistent with the accumulation of CAD mRNA in response to jasmonate application and mechanical wounding. (ox.ac.uk)
Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development. (biomedcentral.com)
Stable transformation in Micro-Tom tomato demonstrated that the candidate promoter regions exhibit differing levels of expression and with varying degrees of fruit specificity. (biomedcentral.com)
Among the five candidate citrus promoters characterized in this study, the CitSEP promoter showed a fruit-specific expression pattern, while the CitWAX and CitJuSac promoters exhibited high fruit-preferential expression with strong activity in the fruit, weak activity in floral tissues and low or undetectable activity in other tissues. (biomedcentral.com)
The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. (biomedcentral.com)
Promoters that are capable of driving fruit-specific or fruit-preferential expression would be valuable tools for engineering improved fruit traits, such as better growth, ripening, nutritional quality, and post-harvest shelf life. (biomedcentral.com)
We show that the transgenic Tpcn1(T159) line lacks expression of both Tpcn1 isoforms in all tissues analyzed. (ox.ac.uk)
None of the three constructs was prone to confer ectopic expression, however, variation of expression between the transgenic lines was observed. (ox.ac.uk)
Using colocalization studies we analysed the degree of concurrence of transgenic and endogenous htr1a expression brought about by these three different constructs. (ox.ac.uk)
To address this, we developed a catalog of MOR promoter (MORp) based constructs packaged into adeno-associated viral vectors that drive transgene expression in MOR+ cells. (bvsalud.org)
UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)
The present invention relates to the designing and synthesis of a completely artificial bidirectional expression module that can also be referred to as bidirectional promoter module. (allindianpatents.com)
The present invention relates to an artificial bidirectional promoter for activation of gene expression in plants. (allindianpatents.com)
Transient over-expression of Fortune Cstps1 promoter in sweet orange showed notable GUS activity, but the Murcott Cstps1 promoter did not. (biomedcentral.com)
Localization of Elements Important for the Wound-Inducible Expression of a Chimeric Potato Proteinase-Inhibitor Ii-Cat Gene in Transgenic Tobacco Plants. (mpg.de)
Cis Regulatory Elements Directing Tuber-Specific and Sucrose-Inducible Expression of a Chimeric Class-I Patatin Promoter Gus-Gene Fusion. (mpg.de)
Expression of Mutant Patatin Protein in Transgenic Tobacco Plants - Role of Glycans and Intracellular Location. (mpg.de)
Tuber-specific gene expression in transgenic potato plants. (mpg.de)
We also searched for spermatogonium-specific promoters and found that the Rcd-1r promoter was able to drive specific expression at this stage. (plos.org)
Contrary to expectations, a fragment containing the entire region between the TBPH gene and the bgcn translational start drove strong HEG expression only during late spermatogenesis rather than in the germline stem cells and spermatogonia as expected. (plos.org)

In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

To date, a number of fruit-specific promoters have been isolated and characterized from various plant species. (biomedcentral.com)

This site-specific or 'targeted' nature of genome editing is typically what makes genome-editing different to traditional 'genetic modification' which inserts a transgene at a non-specific location in the organisms' genome, as well as gene-editing making small edits to the DNA already present in the organisms, verses genetic modification insertion 'foreign' DNA from another species. (wikipedia.org)
Transgenic organisms are living organisms whose genetic material has been artificially modified to introduce specific traits or characteristics. (tutorialspoint.com)
Transgenic organisms have become increasingly important in various fields, including agriculture, medicine, and biotechnology. (tutorialspoint.com)
Genetic and molecular analyses are essential in understanding the mechanisms underlying transgenesis and in evaluating the potential risks and benefits associated with the use of transgenic organisms. (tutorialspoint.com)
PCR can be used to detect the presence of the foreign DNA sequences in transgenic organisms. (tutorialspoint.com)
The use of transgenic organisms has both potential benefits and risks. (tutorialspoint.com)
To evaluate the potential risks and benefits associated with the use of transgenic organisms, it is important to conduct thorough risk assessments and to carefully monitor the effects of transgenic organisms on both the environment and human health. (tutorialspoint.com)
Transgenic organisms have the potential to interact with wild populations and alter the ecological balance of an ecosystem. (tutorialspoint.com)
This can occur through gene flow, which is the transfer of genetic material from transgenic organisms to wild populations. (tutorialspoint.com)
Organisms which have altered or foreign genetic material, are now being referred to as transgenic. (freeonlineresearchpapers.com)

Only the transgenes tlp and bar, under the control of the ubiquitin promoter-intron, were expressed. (elsevierpure.com)
Construction of an Intron-Containing Marker Gene - Splicing of the Intron in Transgenic Plants and Its Use in Monitoring Early Events in Agrobacterium-Mediated Plant Transformation. (mpg.de)

The fragment contains the transcribed region plus 11.5 kb 5'-flanking and 5.5 kb 3'-flanking sequences including all known cis-regulatory elements and the 5' and 3' attachment elements (A-elements) which define the borders of the DNase I sensitive chromatin domain. (eur.nl)
Functional characterization of the P. glauca CAD promoter used P. glauca seedlings stably transformed with a DNA fragment of 1,163 base pairs (PgCAD) fused to the beta-glucuronidase (GUS) gene. (ox.ac.uk)
Here, we further define a 2.5-kb zebrafish nphs2 promoter fragment previously described and identify a 49-bp podocyte-specific transcriptional enhancer using Tol2-mediated G0 transgenesis in zebrafish. (ox.ac.uk)
In addition, by re-inserting the 12-nucleotide fragment, the activity of the Murcott Cstps1 promoter was mostly recovered. (biomedcentral.com)

Dr. Wurm reported that 70% of the $20-billion to $30-billion annual bioproducts inventory is generated with mammalian cells, and the gap separating them from other approaches (yeast, bacteria, baculovirus, transgenic plants, and animals) is only growing wider. (genengnews.com)

The functions of J gene were confirmed by transgenic complementation and near-isogenic lines: the j genotype showed later flowering and maturity date and more yield potential than the J genotype. (chinbullbotany.com)
Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
In our lab, we have generated transgenic lines that express the Green Fluorescent Protein (GFP) ectopically. (oregonstate.edu)
Homozygous transgenic RNAi lines at T 4 seeds with a decreased transcript of TaIPK1 showed 28-56% reduction of the PA. (frontiersin.org)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

Using the chromatin immunoprecipitation assay followed by real-time quantitative PCR, I show that the slowpoke promoter has a distinct spatio-temporal pattern of histone acetylation following a single sedative dose of ethanol. (utexas.edu)
Yeast one-hybrid (Y1H) experiments suggested that LsARF3 could physically interact with the LsCO promoter, which was further confirmed by a dual luciferase assay in tobacco leaves. (frontiersin.org)
Using a transgenic assay, we identified at least five DNA fragments from the Scr gene that silence transcription from a reporter gene. (nih.gov)

Sequence analysis and functional characterization of the promoter of the Picea glauca Cinnamyl Alcohol Dehydrogenase gene in transgenic white spruce plants. (ox.ac.uk)
Unfortunately, the generation of transgenic plants is, in most cases, necessary to conduct the gene editing. (oregonstate.edu)
A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants. (allindianpatents.com)
After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T 1 , T 2 and T 3 generations expressing the tlp gene than in non-transformed control plants. (elsevierpure.com)
Antisense Rna Efficiently Inhibits Formation of the 10 Kd Polypeptide of Photosystem-Ii in Transgenic Potato Plants - Analysis of the Role of the 10 Kd Protein. (mpg.de)
Translatability of a Plant-Messenger Rna Strongly Influences Its Accumulation in Transgenic Plants. (mpg.de)

For example, if a transgenic organism has been modified to express a fluorescent protein, PCR can be used to amplify the DNA sequence encoding the protein and confirm its presence in the organism's genome. (tutorialspoint.com)
introduction of specific genetic material from a donor into a host organism. (freeonlineresearchpapers.com)
The reported transgenesis technique and annotated genome series will permit advanced genetic research on stem cells and regeneration using being a model organism. (careersfromscience.org)
Genetic studies in this organism are not practical because of the tetraploid nature of the genome and the long generation time of this species. (biomedcentral.com)

This is a 1.98kb long DNA sequence containing the ubiquitin promoter and 5' untranslated region from Zea mays. (cbd.int)

Genome editing is characterised by making small edits to the genome at a specific location, often following cutting of the target DNA region by a site-specific-nuclease such as CRISPR. (wikipedia.org)
Genetic modification usually describes the insertion of a transgene (foreign DNA, i.e. a gene from another species) into a random location within the genome. (wikipedia.org)
Another method of genetic analysis is Southern blotting, which is used to detect the presence and copy number of specific DNA sequences in an organism's genome. (tutorialspoint.com)
Two features of X. laevis that have hindered genetic studies are the long generation time (1 to 2 years) and the tetraploid genome of this species. (biomedcentral.com)

The four transgenes co-segregated in the T 1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. (elsevierpure.com)
These fluorescent spotlights on the chromosomes represent the genetic region that codes for the protein of interest. (genengnews.com)

Biotech promoters always claim the expansion of soybean cultivation as a measure of the successful adoption of the transgenic technology by farmers. (i-sis.org.uk)
The study leads to a new direction in genetic research of growth periods for the expansion of soybean to tropical regions. (chinbullbotany.com)

Previous studies of the murine htr1a have revealed that its proximal promoter is GC rich and TATA-less. (ox.ac.uk)
Characterization of the chromosomal rearrangements shown in Figure 1 also revealed that two genetic elements (proximal and distal MES [maintenance elements for silencing]), about 70 kb apart in the Scr gene, must be in cis to maintain proper repression. (nih.gov)
The regions that include the proximal and distal MES are indicated by horizontal arrows. (nih.gov)

Lewy bodies which are intraneuronal aggregates composed mainly of misfolded alpha synuclein (a-syn) protein is a pathological hallmark seen in both sporadic and genetic forms of PD. (lu.se)

These genetic elements required for GT may be assembled through conventional molecular cloning in bacteria. (wikipedia.org)
This requires a combination of genetic, molecular, and ecological analyses. (tutorialspoint.com)
It is well known that anesthetics and abused drugs preferentially modulate specific molecular pathways in defined regions of the brain. (utexas.edu)
However, the molecular mechanisms and genetic contributions behind the regulatory programs that drive neuroma formation-as well as the resulting neuropathic pain-remain incompletely understood. (bvsalud.org)
Modern genetic techniques have helped us hugely in characterizing these disorders and providing a molecular diagnosis in the face of nonspecific clinical data. (medscape.com)

The rapid accumulation of genetic resources for this animal and the success of pilot mutagenesis screens have helped propel this model system forward. (biomedcentral.com)

Transgenic lettuce overexpressing LsARF3 displayed early bolting and flowering, whereas knockout of LsARF3 dramatically delayed bolting and flowering in lettuce under normal or high temperature conditions. (frontiersin.org)

Using the binary GAL4/UAS system to express slowpoke in different parts of the Drosophila nervous system I show that slowpoke induction in the mushroom bodies of the adult fly brain, a region critical for olfactory learning and memory, is important for ethanol tolerance. (utexas.edu)

Promoter is the portion of DNA sequence on 5' side, i.e. before beginning of the coding region of a gene. (allindianpatents.com)
Cloning the coding regions of Cstps1 from Fortune and Murcott mandarin, and aligning with previous reported Valencia orange Cstps1 sequence, showed that they both exhibited extremely high similarity with the known Cstps1 . (biomedcentral.com)

In recent years, these powerful genetic tools have been used in higher vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. (biomedcentral.com)
A genetic toolbox of 'cut-and-paste' DNA transposable elements is now available for use in vertebrates and includes Tol2 and piggyBac . (biomedcentral.com)

The relationship between gene targeting, gene editing and genetic modification is outlined in the Venn diagram below. (wikipedia.org)
In such cases the edits caused by gene-targeting would, in some jurisdictions, be considered as equivalent to Genetic Modification as insertion of foreign DNA has occurred. (wikipedia.org)
DNA microinjections is a common technique used for gene targeting as it involves manipulation and reintroduction of the genetic material into the same species. (freeonlineresearchpapers.com)

The most common method of genetic analysis is polymerase chain reaction (PCR), which is used to amplify specific DNA sequences. (tutorialspoint.com)
Transposable elements (TEs) are mobile genetic sequences that comprise around 50% of our genomic DNA. (lu.se)

Promoter fragments greater than 4.5 kb in length were active in 5-HT(1A) receptor mRNA positive cells and inactive in 5-HT(1A) receptor mRNA negative cells. (ox.ac.uk)

Three binary vectors, designated as p1380-FortP-GUSin, p1380-MurcP-GUSin and p1380-MurcP(+ 12)-GUSin, were developed for promoter activity analysis. (biomedcentral.com)

The µ-opioid receptor (MOR), also known as OPRM1, is highly expressed in several brain regions and the most targeted opioid receptor for pain management. (nih.gov)

Lentivectors stimulate potent immune responses to antigen transgenes and are being developed as novel genetic vaccines. (ox.ac.uk)

Genetic manipulation of this tractable model system would further enhance the use of the frog in developmental studies. (biomedcentral.com)

The enhancer is not only able to activate transcription of the Scr promoter that is 35 kb distant but can also activate transcription of the Scr promoter on the homologous chromosome. (nih.gov)

Public view of transgenic animals is that they maybe harmful to both humans and the environment. (freeonlineresearchpapers.com)

Additional phenotypic characterization is recommended for brain region and experimental paradigm of interest. (nih.gov)

Analysis of the murine 5-HT receptor gene promoter in vitro and in vivo. (ox.ac.uk)
The use of mMORp for in vivo fiber photometry, behavioral chemogenetics, and intersectional genetic strategies is also demonstrated. (bvsalud.org)
To model the effect in vivo, the authors used a transgenic mouse which over-expressed UMOD leading to salt-sensitive hypertension and interstitial nephritis . (medscape.com)

Both types of promoter elements modulate the level of transcription from core promoter [(Wasylyk, B. CRC, Critical Rev. Biochem, 23: 77-120 (1988), Johnson & McKnight Ann. (allindianpatents.com)

Despite the importance of the dermis in the structural and functional integrity of the skin, genetic analysis of dermal development in different parts of the embryo is incomplete. (biologists.com)

Anatomy19

Organisms19

Diseases3

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes95

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Mice22

Genes18

Proteins8

Upstream6

Overexpressing transgenic mouse1

Established transgenic lines1

Transcription Factors3

Tools and transgenic2

Phenotype1

Neurons3

Tissue-specific5

Constitutive5

Expression26

Tissues1

Isolated and characterized1

Organisms10

Intron2

Fragment4

Mammalian1

Lines5

Assay3

Plants6

Organism4

Ubiquitin1

Genome4

Fluorescent2

Expansion of soybean2

Proximal3

Protein1

Molecular5

Accumulation1

Knockout1

Drosophila1

Sequence2

Vertebrates2

Gene Targeting3

Sequences2

Fragments1

Vectors1

OPRM11

Transgenes1

Developmental1

Homologous1

Humans1

Characterization1

Vivo3

Modulate1

Functional1