Transgene gene flow. Medical search. Frequent questions

In particular, a unique method of intra-arterial gene transfer under vascular isolation that results in highly efficient and localized transgene expression will be used for gene transfer and laser Doppler perfusion imaging to measure dermal blood flow will facilitate the accomplishment of the above aims. (nih.gov)

Flow cytometry and fluorescent microscopy revealed expression of mCherry in diverse cell types, accounting for more than 90% of adult animal cells. (iucc.ac.il)
We show that flow cytometry enabled quantification of the relative expression conferred by the number of transgene integrations, and allowed the quick identification of clones containing single-allele, double-allele, and random integrations. (nih.gov)
Among the array of analytical techniques utilized in CAR T cell assessment, flow cytometry stands out as a powerful and versatile tool. (biobostonconsulting.com)
Flow cytometry enables researchers to simultaneously analyze multiple parameters, offering critical insights into various CAR T cell attributes throughout the manufacturing process. (biobostonconsulting.com)
Key parameters that can be assessed through flow cytometry include cell viability, identity, purity, and strength, all of which are crucial for ensuring the safety and efficacy of CAR T cell therapies. (biobostonconsulting.com)
To achieve robust and reliable results, flow cytometry assays should be developed using scientifically sound principles. (biobostonconsulting.com)
Properly validated flow cytometry assays can provide accurate and consistent data on CAR T cell characteristics, paving the way for informed decision-making during preclinical and clinical development. (biobostonconsulting.com)
For the initial submission of an Investigational New Drug (IND) application, a detailed description of the flow cytometry assay should be provided. (biobostonconsulting.com)
Increased transduction efficiency was determined by performing GFP-based flow cytometry, and the expression of the TK protein by the retargeted adenovirus was assessed by performing an immunohistochemical analysis focused on HSV-TK. (medscape.com)
3. Flow cytometry for assessment of the efficacy of siRNA. (nih.gov)
The Flow Cytometry Core is a state-of-the-art facility to accommodating a wide range of cell sorting and cell analysis needs for basic and translational research for NHLBI investigators in a cost-efficient time bound manner. (nih.gov)
This Core supports a wide variety of applications, including high speed cell sorting, 30-color cell analysis, cytokine analysis by multiplex bead arrays (Luminex), imaging flow cytometry (Amnis Image-Stream), phosphoprotein analysis, cell cycle and ploidy analysis, protocol standardization / methods development for high dimensional flow cytometric assays for longitudinal studies. (nih.gov)
This includes the use of flow cytometry, diagnostic PCRs, qRT PCR and southern blotting. (hdac-assay.com)
Assessment of T Cell Development by Flow Cytometry. (nih.gov)

7.5 kb), and can achieve high levels of transgene expression. (medscape.com)

Transgenes and conventional genes are subject to the biological processes of mutation, gene flow, introgression, recombination and natural selection. (cgiar.org)
Therefore, best practices for preventing introgression of conventional genes also provide an appropriate basis for preventing introgression of transgenes. (cgiar.org)
This finding suggests that glyphosate resistance might come from GM oilseed rape crops illegally cultivated in the country or as a seed contaminant, and it implies gene flow and introgression between feral populations of GM B. napus and wild B. rapa. (uchile.cl)
However, a neglected factor, gene introgression from domesticated species through repeated outcrossing, may have a significant impact on CWR diversity. (jse.ac.cn)
When largely enhancing or reducing fitness of wild plants, the introgression of crop genes will impose more significant genetic and evolutionary impacts on CWR populations, leading to undesired consequences for conserved CWR populations and species. (jse.ac.cn)
This article reviews the challenge of crop-wild gene flow, and particularly transgene introgression from GE crops, for the in situ conservation of wild relative species. (jse.ac.cn)
This article explores the trajectory of the global controversy over the introgression (or not) of transgenes from genetically modified maize into Mexican indigenous maize landraces. (naturalezacienciaysociedad.org)

In addition, plastid transformation has the advantage that the site of gene insertion can be controlled, high rates of transgene expression and protein accumulation can be achieved and epigenetic effects are absent. (uni-muenchen.de)
Spatial patterns of gene expression in living organisms orchestrate cell decisions in development, homeostasis, and disease. (nih.gov)
We introduce a depth- and scale-flexible method to direct volumetric gene expression patterning in 3D artificial tissues, which we call "heat exchangers for actuation of transcription" (HEAT). (nih.gov)
We show that gene expression patterning can be tuned both spatially and dynamically by varying channel network architecture, fluid temperature, fluid flow direction, and stimulation timing in a user-defined manner and maintained in vivo. (nih.gov)
Transgene expression in tick cells using Agrobacterium tumefaciens. (cdc.gov)
We demonstrate that co-transfection of the crRNA:tracrRNA duplex into Cas9-expressing cells leads to target gene mutation and loss of target protein expression in the majority of the cell population. (biomedcentral.com)
To this end, we have generated multiple human iPSC lines by gene tareting at two defined loci that allow constitutive expression of the inserted reporter genes. (nih.gov)
Adenovirus transduction in gene therapy is dependent on the expression of the coxsackie virus adenovirus receptor (CAR) for initial binding and on the integrin receptors (αvβ3, αvβ5) for viral internalization. (medscape.com)
2. Nonviral genetic modification mediates effective transgene expression and functional RNA interference in human mesenchymal stem cells. (nih.gov)
5. Downregulation enhanced green fluorescence protein gene expression by RNA interference in mammalian cells. (nih.gov)
12. A tightly regulated and reversibly inducible siRNA expression system for conditional RNAi-mediated gene silencing in mammalian cells. (nih.gov)
15. Stable suppression of gene expression in murine embryonic stem cells by RNAi directed from DNA vector-based short hairpin RNA. (nih.gov)
Stem cells in tissue are rare and reside in particular environmental niches that make it difficult to assess gene expression without contamination from surrounding tissue. (rosalindfranklin.edu)
To identify and collect spatially restricted samples in tissue, the center has a Leica Laser microdissection system and equipment for downstream analysis of gene expression by quantitative PCR. (rosalindfranklin.edu)
In vitro, CD4-LV demonstrated absolute receptor specificity on cell lines and the naturally mixed population of primary peripheral blood mononuclear cells (PBMC), with transgene expression exclusively detectable in CD4+ but not CD4- cells. (tu-darmstadt.de)
This study aimed to determine whether suppression of G6p2 gene expression might therefore prevent or delay disease progression. (diabetesjournals.org)
Since a combination of TGS and PTGS effects can lead to a progressive shut down of transgene expression, we Inhibitors,Modulators,Libraries were inter ested in finding early indicators and methods to avoid or even predict unwanted Inhibitors,Modulators,Libraries transgene silencing in the wild tobacco N. attenuata. (hdac-assay.com)
And the low methylation status of MUC2 gene plays a predominant role in high level MUC2 expression in mu cinous colorectal cancer. (hdac-assay.com)
The histone H3 modifica tion could play an important role in MUC2 gene expression, possibly affecting DNA methylation in pan creatic neoplasm. (hdac-assay.com)
One major limitation of this method Is decreased recombinant gene expression due to host Immunity. (ox.ac.uk)
To assess the relative Importance of host factors In reducing transgene expression In-vlvo, we performed adenoviral gene transfer of B-Galactosldase (B-Qal) to rabbit carotid arteries (CA), Gene transfer was carried out to both CA using passive dwell for 15 min using liters of 1x10", 2x10'°, or 8x10 pfu/ml. (ox.ac.uk)
Results: Vessels from 6 rabbits were studied lor B-Gal expression 3 days after gene transler. (ox.ac.uk)
Conclusions: Adenoviral gene transfer to rabbit CA was highly efficient, with only minor differences in expression across the three tilers. (ox.ac.uk)
Therefore, host responses to adenoviral-medlated arterial gene transfer markedly reduce transgene expression in-vivo. (ox.ac.uk)
Because efficient gene silencing depends on both the design and level of expression of the shRNA, it is critical to choose silencing reagents where both the targeting sequence and the specific promoter driving expression are taken into consideration. (horizondiscovery.com)
In WD/DDLPS, NCs contain amplified copies of genes including MDM2 , CDK4, SAS, HMGA2 and YEATS4, whose over-expression is thought to drive or contribute to carcinogenesis. (sarcomahelp.org)
NRSEs are short pieces of DNA that suppress neural gene expression in non-neural tissue. (nih.gov)
The team then showed that proteins endogenous to zebrafish were likely binding the NRSE sites and inhibiting transgene expression in non-nervous system tissue. (nih.gov)
2012). Brain Selective Transgene Expression in Zebrafish Using an NRSE Derived Motif. (nih.gov)

The TuBECs were transduced with either a native adenovirus (AdHSV-TK) or a retargeted adenovirus (FGF2-AdHSV-TK), both of which carry the suicide herpes simplex virus thymidine kinase (HSV-TK) gene. (medscape.com)

Transgenic plants carry transgenes in their genomes and transmit them to their progeny through normal reproduction. (cgiar.org)
To reduce the risk in crops where transgenes are commonly part of new cultivars, it is recommended that regeneration be carried out in isolation from any areas where transgenic crops are likely to be grown. (cgiar.org)
Information on crops' transgenic status is essential to determine what measures, if any, are needed to confirm that germplasm is free of transgenes. (cgiar.org)
misc{unal_58526, author = {Chaparro-Giraldo Alejandro and Blanco M. Jennifer Teresa and López-Pazos Silvio Alejandro}, title = {Evidence of gene flow between transgenic and non-transgenic maize in Colombia}, year = {2015-09-01}, abstract = {Maize (Zea mays) is an important crop worldwide and is essential for industry. (unal.edu.co)
We analyzed the gene flow phenomenon of 60 randomly chosen plots of maize, including farmer field landraces or other conventional varieties such as non-transgenic hybrids in Valle de San Juan (Colombia) using Inmunostrip®, PCR and ELISA tests on leaves (seed gene flow) and seeds (pollen gene flow). (unal.edu.co)
More than 88% of the plots were positive with the Inmunostrip® and PCR tests (35S promoter, Nos terminator and cry1F gene), using the leaves, while the remaining seven plots (12%) were positive for transgenic sequences in the seeds. (unal.edu.co)
The results indicated a significant level of overall transgene existence, which is consistent with gene flow from transgenic events. (unal.edu.co)
There are many problems that could increase the gene flow potential in Valle de San Juan, such as little respect for regulations (Colombian Decree 4525 on transgenic crops and biosafety), distance between transgenic and non-transgenic maize or use of refuge and/or buffer zones, high seed reuse and exchange and low technical assistance. (unal.edu.co)
TY - GEN T1 - Evidence of gene flow between transgenic and non-transgenic maize in Colombia AU - Chaparro-Giraldo, Alejandro AU - Blanco M., Jennifer Teresa AU - López-Pazos, Silvio Alejandro Y1 - 2015-09-01 UR - https://repositorio.unal.edu.co/handle/unal/58526 PB - Universidad Nacional de Colombia AB - Maize (Zea mays) is an important crop worldwide and is essential for industry. (unal.edu.co)
Described in the journal Nature , the Beijing team created their transgenic monkeys by using a virus to transfer a human gene called MECP2 into egg cells of cynomolgus monkeys, or the crab-eating macaque. (iflscience.com)
Last but not least, five offspring were produced from one of the male transgenic monkeys, all of which had the human MECP2 gene and showed increased levels of the protein it codes for in their brains. (iflscience.com)

therefore, plastid genetic engineering is a promising tool to reduce the risk of transgene flow, because in most angiosperm species plastids are inherited maternally. (uni-muenchen.de)
Regarding genetic stability, both genes segregate according to Mendelian rules of inheritance for a single genetic locus. (fao.org)
More information on Transgenes can also be found at the genetic identity page under Management strategies). (cgiar.org)
Social issues requiring genetic identity to be stated or that certain genes/transgenes be limited. (cgiar.org)
Germplasm is most at risk from gene flow during regeneration (see also information on Regeneration ) and controlling gene flow is essential to ensure genetic integrity. (cgiar.org)
It is argued that a GE crop usually contains transgenes with strong natural selection advantages, and such transgenes introgressed into CWR populations may have strong impacts on their genetic diversity and evolutionary processes, threatening their conservation. (jse.ac.cn)
For this research we are using the genetic disease, canine leukocyte adhesion deficiency (CLAD), as a model for applying advances in understanding of stem cell biology to enhance gene transfer into the hematopoietic stem cells. (nih.gov)
The ability to correct genetic hematopoietic diseases by gene therapy has a number of advantages over current therapies that involve allogeneic hematopoietic stem cell transplantation (HSCT). (nih.gov)
Establishing an efficient, multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in its application would further advance the adoption of CRISPR/Cas9 for genetic studies. (biomedcentral.com)
Compared to RNA interference (RNAi), CRISPR/Cas9-mediate gene KO has the advantage of creating true genetic nulls instead of hypomorphs. (biomedcentral.com)
Those who eat meat, eggs, and cheese, and drink milk, those who buy food for their pets, those who prefer to eat organic food, those who question the wisdom of inserting into a plant's genetic make-up the genes of a bacterium that confers resistance to a broad-spectrum herbicide-basically all of us, one way or another-should be paying close attention. (ecologicalgardening.net)
Genetic fallout in biocultural landscapes: molecular imperialism and the cultural politics of (not) seeing transgenes in Mexico. (naturalezacienciaysociedad.org)
Hexanucleotide repeat expansions in the C9ORF72 gene are the commonest known genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. (nature.com)
What is viral in Virologist actions is that a early source of the genetic Cry recombination reduces created written into the crop's selected ability, even that the Notice's single-gene leaf is the source. (be-mindful.de)
Despite groundbreaking work in defining the genetic basis for the disease, leading to the description of more than 400 predominantly missense mutations in more than 8 largely sarcomeric genes, therapies remain palliative. (nih.gov)

It is generally accepted as unwise for crops containing transgenes to be incorporated into germplasm collections. (cgiar.org)
Immediate attention: crops with transgenes that are already commercially distributed. (cgiar.org)
Letting the gene out of the bottle: the population genetics of genetically modified crops. (notulaebotanicae.ro)

Transgenes are genes that are introduced into another organism or species through recombinant DNA techniques. (cgiar.org)
Moving genes between species is changing our world. (psgr.org.nz)
The interaction between cultivated genetically modified plants (GMP) and wild plant species could be a transference source of transgenes. (notulaebotanicae.ro)
Biodiversitas encourages submission of manuscripts dealing with all biodiversity aspects of plants, animals and microbes at the level of the gene, species, and ecosystem as well as ethnobiology. (smujo.id)
Plant transformation is a technique that allows us to transfer genes from one species to another in order to introduce new characteristics into the recipient. (intechopen.com)
There are now many established gene-transfer methods, both direct and indirect, for the stable introduction of novel genes into plant species. (intechopen.com)

4. [Detection of RNA interference in nasopharyngeal carcinoma cell lines using reporter genes]. (nih.gov)

These iPSC lines contain lox-flanked CAG driven fluorescent protein genes at the well-defined AAVS1 locus in the PPP1R12C (protein-phosphatase 1 regulatory subunit 12C) on Chr. (nih.gov)

Delivery of functional or reporter transgenes to cells in vitro and in vivo is an important tool for the discovery of stem cell regulation and contribution to repair. (rosalindfranklin.edu)
Elucidation of the actions of these disease genes at a systems level with novel functional and cellular outcomes from our neural circuit mouse model and characterization of additional neurodegenerative disease genes in this model system hopefully will contribute to the development of effective therapies. (nih.gov)

CRISPR/Cas9 mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes simultaneously. (biomedcentral.com)
In this study, we present a simple, versatile and highly efficient method to achieve acute gene knockout with CRISPR/Cas9 using chemically synthesized crRNA and tracrRNA oligos. (biomedcentral.com)
Our method is a convenient yet powerful tool to enable rapid and scalable gene knockout using CRISPR/Cas9 in mammalian cells. (biomedcentral.com)
CRISPR/Cas9 has been adapted for gene knockout (KO) in eukaryotic cells. (biomedcentral.com)

We show that dominant molecular norms of proof and standards of detection, which co-developed with the world of industrial monocropping and gene patenting, discarded and externalized non-compliant actors (i.e. complex maize genomes, human dimensions of gene flow). (naturalezacienciaysociedad.org)

The combination of cellular therapy and gene delivery is an attractive option as it will potentially protect the vector from immune surveillance, and will support targeted delivery of a gene or therapeutic protein to the tumour site. (biomedcentral.com)

In order to deliver a safe and effective product, human gene therapies present many manufacturing challenges. (nih.gov)

Indeed, upon pseudotyping of PTVs with MVwt-GPs, marker gene and GFP, Cre or ovalbumin (Ova) cargo protein delivery was completely restricted to SLAM- and Nectin-4-positive CHO cells and naturally SLAM-expressing B cell lines, whereas broad transduction was observed for VSV-G pseudotyped control vectors. (tu-darmstadt.de)
Beside transduction of CD4+ T lymphocytes, also gene transfer into CD4+ human macrophages could be demonstrated in vitro. (tu-darmstadt.de)

Genebanks should take proactive steps to limit the risk of exotic genes, including transgenes, in their ex situ collections. (cgiar.org)
These analyses employ standard cytogenetic and fluorescence in situ hybridisation (FISH), array-based profiling, and flow cytometric isolation and massively parallel sequencing of WDLPS NCs, complemented by transcriptome sequencing. (sarcomahelp.org)

A pmi gene, allowing transformed maize cells to utilize mannose as a sole carbon source, was used as a selectable marker. (fao.org)
Accessions for which there were no commercial transgenes present at the time of acquisition (such as maize prior to 1996) or no transgenics near the collecting site. (cgiar.org)
However, little is known about the gene flow processes that affect maize fields in Colombia, which is near the center of diversity for cultivated maize. (unal.edu.co)
All of the field types (conventional maize, buffer zones, refuge, and Colombian landraces) showed evidence of a transgene presence. (unal.edu.co)
While a plurality of knowledge-making processes were deployed to render transgenes visible or invisible, we analyze how a particular in vitro based DNA-centered knowledge came to marginalize other forms of knowledge, thus obscuring other bio-cultural dimensions key to the understanding of gene flow and maize diversity. (naturalezacienciaysociedad.org)

This approach leverages fluid-based heat transfer from printed networks in the tissues to activate heat-inducible transgenes expressed by embedded cells. (nih.gov)
This project represents a pre-clinical model for gene transfer into hematopoietic stem cells. (nih.gov)
First, the use of autologous hematopoietic stem cells for gene therapy obviates the need for a matched bone marrow donor since each individual would serve as his or her own bone marrow stem cell donor. (nih.gov)
Second, the use of the patient's own bone marrow cells for gene replacement does not result in graft-versus-host disease (GVHD), a major complication of current allogeneic transplant. (nih.gov)
We have used this model to test retroviral and foamy viral gene transfer into the bone marrow cells of dogs. (nih.gov)
Because RNAi utilizes the endogenous RNA-induced silencing complex (RISC), gene knockdown can be achieved by simply transfecting a synthetic short-interfering RNA (siRNA) duplex into cells, a process that is both highly efficient and easily scalable. (biomedcentral.com)
7. Effective gene suppression using small interfering RNA in hard-to-transfect human T cells. (nih.gov)
Initially, production of PTVs was optimized to allow maximum gene and protein transfer into transduced cells. (tu-darmstadt.de)
In vivo, following systemic injection of CD4-LV transferring a bicistronic gfp/luciferase reporter gene, luciferase activity in NOD-scid IL2Rγ-/- mice reconstituted with human PBMC or hematopoietic stem cells was mainly detected in lymphoid organs. (tu-darmstadt.de)
The aim of the present review is to discuss the range of approaches that have been used to exploit the tumour-homing capacity of mesenchymal stem cells for gene delivery, and to highlight advances required to realize the full potential of this promising approach. (biomedcentral.com)
In the current study, we further characterized TCR:zeta in gene-engineered T cells and assessed whether this receptor is able to interact with surface molecules and drive correct synapse formation in Jurkat T cells. (unideb.hu)

This remarkable combination of properties has formed the basis for many studies investigating their potential as tumour-specific delivery vehicles for suicide genes, oncolytic viruses and secreted therapeutic proteins. (biomedcentral.com)

One of the most important components of proper genebank management is testing for the presence of a gene or phenotype. (cgiar.org)
There are a number of reasons why it is important to detect the presence of a gene/transgene in a genebank accession. (cgiar.org)
Regulatory issues, especially related to phytosanitary or biosafety, where the country of import, and potentially the country of export, requires reporting on the presence of such genes. (cgiar.org)
Situations in which the presence of such a gene/transgene could affect intellectual property rights either in the country where the genebank is located or in a country where the accession is to be sent. (cgiar.org)
The two basic methods to detect the presence of a gene/transgene are ELISA and PCR amplification. (cgiar.org)
Presence of transgenes could be cause and adverse environmental impact on non-target organisms. (notulaebotanicae.ro)
Characterization of this model uncovered a population of mouse olfactory neurons that undergo enhanced cell death and axon mistargeting in a non-cell autonomous fashion in the presence of this AD gene. (nih.gov)

Results Non Mendelian segregation of the resistance marker as the first indicator of transgene silencing To be able to work with N. attenuata lines that constitu tively express antimicrobial peptides under a 35S pro moter, we created eleven different transformation vectors containing eleven different antimicrobial peptide genes. (hdac-assay.com)
The segregation analysis of the resistant marker provides not only Inhibitors,Modulators,Libraries information Inhibitors,Modulators,Libraries about zygosity but can additionally reveal independent segregating loci and the occurrence of unwanted trans gene silencing very early in the screening process. (hdac-assay.com)

The loci utilized are so called "safe harbors" in the genome which, due to their open chromatin structure, allow transcription of the introduced transgenes robustly and persistently. (nih.gov)

Much progress has been gained in identifying mutations and gene products that underlie these disease processes, particularly early onset Alzheimer's disease (AD) and Parkinson's disease (PD). (nih.gov)

Explanation for this could be duplication or redundancy of nuclear genes mediating maternal inheritance and suppressing paternal leakage in Arabidopsis in such a way that the defect in one gene is compensated for by the function of its homologue. (uni-muenchen.de)
Gene paralog redundancy is a common phenomenon in the mammalian genome. (biomedcentral.com)

The aim of this study was to assess the frequency of plastid transfer from atrazin-resistant ElyF3BC4 Arabidopsis thaliana plants bearing a point mutation in the plastid psbA gene to male sterile N75 plants by spontaneous crossing under field conditions. (uni-muenchen.de)

2023. An androgen receptor regulated gene score is associated with epithelial to mesenchymal transition features in triple negative breast cancers. . (ncbs.res.in)

We generated animals containing a TATA binding protein (TBP) promoter upstream of the mCherry gene. (iucc.ac.il)
Canadian researchers created the fast-growing genetically engineered salmon with a growth hormone gene from Chinook salmon and a gene promoter from ocean pout.They microinjected the transgene into fertilized eggs of wild Atlantic salmon, and characterized the insertion. (siaweddings.com)
MUC2 gene regulation mechanism disclosed that DNA methylation and his tone modification in the 5 flanking region of the MUC2 promoter may play an important role. (hdac-assay.com)
Guaranteed gene silencing with state-of-the-art shRNA designs and multiple promoter and reporter options. (horizondiscovery.com)

11. In vivo gene silencing in solid tumors by targeted electrically mediated siRNA delivery. (nih.gov)

The vectors and methods identified in these studies will be used in future clinical trials of ex vivo gene therapy. (nih.gov)
To date ex vivo gene therapy in hematopoietic disease has only been successful in diseases in which the transgene carries a selective advantage. (nih.gov)

Recombinant adenovtruses have emerged as a promising vehicle for in-vlvo gene therapy. (ox.ac.uk)

He also says that environmental risks have not been adequately evaluated, including the effect of gene-flow on biological diversity. (org.in)
This guidance also applies to combination products that contain a human gene therapy biological product in combination with a drug or device as part of the final product. (nih.gov)

9. Sequence-specific gene silencing in murine muscle induced by electroporation-mediated transfer of short interfering RNA. (nih.gov)
Microinjection is a direct type of gene transfer. (mybiologydictionary.com)

Adenoviral vectors have been extensively used in laboratory experiments and clinical trials for GBM gene therapy. (medscape.com)
There have been recent published reports of gene therapy clinical trials in which adenoviral vectors have been used in the treatment of GBM, including the multicenter trial of ONYX-015 conducted by Chiocca, et al. (medscape.com)
The use of viral vectors has become increasingly important in cell culture for gene therapy applications and requires a reliable, reproducible, and efficient scale-up platform to streamline time to market. (corning.com)
Despite significant advances in the field of gene therapy for cancer, two major obstacles remain that continue to limit the clinical potential of this approach: lack of tumour tropism of vectors, and stimulation of an immune response. (biomedcentral.com)

Up to ten shRNA silencing constructs are predesigned for every gene in human, mouse and rat providing broad coverage across the entire genome. (horizondiscovery.com)

Review a bibliography of published papers supporting the use of Corning cell expansion cultureware in cell, immune, and gene therapy applications. (corning.com)

His Microglia research includes themes of CX3CR1, Transcriptome, Experimental autoimmune encephalomyelitis, Gene and Neurogenesis. (research.com)
We have developed an experimental approach to elucidate the actions of genes that cause neurodegenerative disease in a single mammalian neural circuit. (nih.gov)
In addition, we propose to extend this experimental approach to genes associated with other neurodegenerative diseases such as PD. (nih.gov)

We also show that delivering three crRNAs targeting EGFP, KRAS and PTEN in the same reaction leads to the simultaneous knockout of all three genes. (biomedcentral.com)

As the name suggests, it's caused by a DNA duplication event on part of the X chromosome that carries the MECP2 gene , which is thought to play a key role in facilitating brain cell communication by maintaining the connections across which information flows. (iflscience.com)

This guidance document provides to you, sponsors of a human gene therapy investigational new drug application (IND), recommendations on the chemistry, manufacturing, and control (CMC) information to include in an original IND. (nih.gov)
Modified crab-eating macaques can pass on human genes to offspring. (iflscience.com)
After infecting the eggs, they were artificially inseminated and transferred to surrogate mothers, and all of the successful pregnancies resulted in offspring with the human gene. (iflscience.com)

Ldb1-nucleated transcription complexes function as primary mediators of global erythroid gene activation. (nih.gov)

Posted on August , 2017 in Alagna F( 2013) Innovative Transcriptomics Approaches for Large Scale Identification of Genes Involved In Plant medical download Facility Piping Systems Handbook: For Industrial, Commercial, and Healthcare. (be-mindful.de)

6. Selection and validation of optimal siRNA target sites for RNAi-mediated gene silencing. (nih.gov)

Using mouse genetics, we expressed an AD gene exclusively in primary olfactory sensory neurons in a conditional manner. (nih.gov)

This is critical for various phytosanitary requirements, but is also becoming important for the detection of transgenes. (cgiar.org)
1. Choice of the adequate detection time for the accurate evaluation of the efficiency of siRNA-induced gene silencing. (nih.gov)

While these findings have led to the development of mouse models of these diseases, little is known at the systems level about how these disease genes impact the function of a specific neural circuit. (nih.gov)

The ever-changing landscape of gene therapy has led to therapeutic developments for conditions once thought untreatable, as well as improvements in existing medical interventions. (corning.com)
Look to our decades-long expertise in cell culture product development, and our knowledgeable field application support specialists, to help guide your next gene therapy discovery. (corning.com)
T-cell receptors (TCRs) can be genetically modified to improve gene-engineered T-cell responses, a strategy considered critical for the success of clinical TCR gene therapy to treat cancers. (unideb.hu)

13. Cell lines retained their pluripotency after gene-targeting, as assayed by staining for pluripotency markers and by in vitro spontaneous differentiation via embryoid body formation. (nih.gov)

Anatomy5

Organisms36

Diseases1

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Psychiatry and Psychology4

Phenomena and Processes65

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena4

Technology, Industry, Agriculture5

Humanities2

Information Science3

Health Care17

Geographicals17

Localized transgene expression1

Cytometry14

Levels of transgene expression1

Introgression7

Expression29

Herpes simplex virus thymidin1

Transgenic11

Genetic15

Crops3

Species6

Reporter genes1

AAVS11

Functional2

CRISPR4

Genomes1

Cellular1

Therapies1

Transduction2

Situ2

Maize5

Cells11

Proteins1

Presence7

Marker2

Loci1

Mutations1

Redundancy2

Point mutation1

Mesenchymal1

Promoter4

Tumors1

Vivo gene2

Recombinant1

Biological2

Transfer2

Vectors4

Constructs1

Immune1

Experimental3

Knockout1

Carries1

Human3

Transcription1

Plant1

RNAi1

Exclusively1

Detection2

Diseases1

Therapy3

Differentiation1