Transform adenoviruses human. Medical search. Frequent questions

Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
erbB-2 overexpression in human mammary epithelial cells confers growth factor independence. (musc.edu)
Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells. (musc.edu)
Activation of Src by protein tyrosine phosphatase 1B Is required for ErbB2 transformation of human breast epithelial cells. (musc.edu)
Cooperative interactions of HER-2 and HPV-16 oncoproteins in the malignant transformation of human mammary epithelial cells. (musc.edu)

Gene and mRNA for precursor polypeptide VI from adenovirus type 2. (microbiologyresearch.org)
The gene for the adenovirus 2 hexon polypeptide. (microbiologyresearch.org)
Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region. (uu.nl)
Consequently, gene therapy products are transforming the treatment of cancers and genetic diseases. (kaloramainformation.com)
Many gene therapy clinical trials rely on retroviruses or adenoviruses to deliver the desired gene. (kaloramainformation.com)
This indicates that an adenovirus gene located outside the classic transforming region (E1) can significantly influence the in vivo oncogenicity of an adenovirus. (princeton.edu)
PDGF-receptor b -targeted adenovirus redirects gene transfer from hepatocytes to activated stellate cells. (rug.nl)
Amazingly, the first human cancer gene was cloned only thirty years ago. (jcancer.org)
Without doubt, the first human transforming gene, and more broadly, oncogenes and anti-oncogenes, had been previously theorized by Theodor Boveri. (jcancer.org)
Results: FGS of tumours in the lung was enabled by labelling with a telomerase-dependent adenovirus containing the GFP gene. (elsevierpure.com)

The role of adenovirus structural proteins in the regulation of adenovirus infection. (microbiologyresearch.org)
The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)

These cell lines were transformed with origin-defective adenovirus/SV40 recombinant virions, and IJC was determined both between transformed cells (homologous IJC) and between transformed and untransformed cells (heterologous IJC). (ox.ac.uk)

Processing of adenovirus 2 induced proteins. (microbiologyresearch.org)
We conclude that the association and altered regulation of IRS proteins by Ad5E1A contribute to the adenovirus-transformed phenotype and modulates viral infection in an Akt-dependent manner. (ox.ac.uk)
This review discusses the interaction between proteins of several human oncogenic viruses with the Wnt signaling pathway. (ac.ir)

Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973. (wikipedia.org)
HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
The recombinant virus replicated efficiently in human embryonic kidney cells and HeLa cells, showing that the transforming regions of Ad5 and Ad12 had similar functions in productive infection. (uu.nl)
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5. (nih.gov)

Given the location of the adrenal gland (adrenal means "next to the kidney"), a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. (wikipedia.org)
4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin. (cellosaurus.org)

Adenovirus protein- protein interactions: hexon and protein VI. (microbiologyresearch.org)
The mRNA vaccines contain only the code for the SARS-CoV-2 envelope spike protein, whereas the DNA-based vaccines both contain an adenovirus viral vector that has been augmented with DNA that codes for the SARS-CoV-2 spike protein. (theepochtimes.com)
But a disadvantage is that those who have been exposed to natural forms of the adenovirus have antibodies to the virus that will likely block the synthesis of the spike protein, and therefore not afford protection against SARS-CoV-2. (theepochtimes.com)
Growth/differentiation factor-5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family, which is a subgroup of the transforming growth factor- (TGF-) superfamily. (ispub.com)
Using mass spectrometric analysis insulin receptor substrate 4 (IRS-4) has been identified as a novel adenovirus 5 early region 1A (Ad5E1A)-binding protein. (ox.ac.uk)
Prolonged expression of Ad5E1A13S is required for the observed dramatic increase in the levels of IRS-4 mRNA and protein in Ad5E1-transformed human cell lines. (ox.ac.uk)
FIG. 11 depicts enzyme-linked immunosorbent assay (ELISA) measurements of human glial-derived neurotrophic factor (hGDNF) protein in retinal homogenates 2 and 3 months following intravitreal delivery of ShH10.Y445F.scCAG-hGDNF. (justia.com)
Methods: Human lung cancer cell lines H460 and A549 were genetically transformed to express red fluorescent protein (RFP). (elsevierpure.com)
After the tumours reached 5 mm in diameter, they were injected under fluorescence guidance with the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus, OBP-401. (elsevierpure.com)
Transforming growth factor-β (TGFβ) plays a pivotal role in extracellular matrix production, but bone morphogenetic protein (BMP)-7, a member of the TGFβ superfamily, can antagonise the fibrogenic activity of TGFβ. (mssm.edu)

Characterization of the adenovirus proteinase: substrate specificity. (microbiologyresearch.org)
Characterization of murine pregnancy decidua transforming growth factor beta. (mcmaster.ca)

Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. (uu.nl)

It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)

Telomeres shorten during ageing of human fibroblasts. (cshlpress.com)
Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. (ox.ac.uk)
The effect of SV40 viral transformation of human fibroblasts on intercellular gap junctional communication (IJC) was investigated using a short-term quantitative assay. (ox.ac.uk)
IJC was measured using metabolic cooperation in a coculture system of argininosuccinate synthetase- and argininosuccinate lyase-deficient human fibroblasts. (ox.ac.uk)
We conclude that SV40 transformation of human fibroblasts leads to a partial impairment of IJC which is additive when both communicating partners are transformed. (ox.ac.uk)

The development of a vaccine against human cytomegalovirus infection (HCMV) is a high-priority medical goal. (asm.org)
Congenital human cytomegalovirus infection (cCMV) is a major cause of childhood disease, often leading to permanent neurologic sequelae ( 1 ). (asm.org)
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
AAV belongs to the Parvoviridae family and Dependovirus genus, whose members require co-infection with a helper virus such as adenovirus to promote replication, and AAV establishes a latent infection in the absence of a helper. (justia.com)
Studies in humans and animal models have shown that symptoms are not immediate and appear days or even weeks after infection. (shengsci.com)
Methods and results: In primary cultured rat stellate cells and the LX-2 human stellate cell line, induction of BMP-7 by Ad-BMP-7 infection decreased the expression of collagen 1A2 mRNA and smooth muscle α-actin in the presence or absence of TGFβ, via Smad 1/5/8 phosphorylation. (mssm.edu)

Subsequent analysis has shown that the transformation was brought about by inserting ~4.5 kilobases from the left arm of the viral genome, which became incorporated into human chromosome 19. (wikipedia.org)
The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
The recombinant genome consists essentially of Ad5 sequences, with the exception of the transforming early region 1 (E1) which is derived from Ad12. (uu.nl)
The amounts of different regions of the SV40 genome present in a line of transformed mouse cells. (cshl.edu)
Study of Sequences of Adenovirus-2 DNA in Nine Lines of Transformed Rat Cells Using Specific Fragments of Viral Genome. (cshl.edu)

Lack of functional pRb results in attenuated recovery of mRNA synthesis and increased apoptosis following UV radiation in human breast cancer cells. (musc.edu)

More than 100 serologically distinct types of adenovirus have been identified including 49 types that infect humans. (biologydiscussion.com)

Impaired antiviral response of adenovirus-transformed cell lines supports virus replication. (probiogen.de)
When amplifying purified adenovirus that has been serially propagated it may be wise to isolate viral clones (with a plaque amplification), in order to avoid any possible problems with replication-competent adenovirus. (umich.edu)

However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
SV40 transformation of human diploid cells. (cellosaurus.org)
Human diploid lung fibroblast cell lines WI 26 and WI 38 exhibit isozyme shift of alkaline phosphatase after viral transformation. (cellosaurus.org)
Oncogene activation induces metabolic transformation resulting in insulin-independence in human breast cancer cells. (musc.edu)

Kelly, F. , Sambrook, J. (1974) Variants of Simian Virus 40-Transformed Mouse Cells Resistant to Cytochalasin-B. Cold Spring Harbor Symposia on Quantitative Biology, 39. (cshl.edu)

Papovaviruses are one of the four important dsDNA viruses (e.g. papovaviruses, adenoviruses, herpes viruses and pox viruses) which produce tumour in many animals. (biologydiscussion.com)
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains. (cellosaurus.org)

Viral DNA and a viral peptide are cofactors of adenovirus virion proteinase activity. (microbiologyresearch.org)
The adenovirus protease is activated by a virus-coded disulphide-linked peptide. (microbiologyresearch.org)

A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
Respiratory viruses cause disease in humans characterized by an abrupt onset of symptoms. (shengsci.com)
Several adenoviruses cause respiratory and conjunctival diseases such as pneumonia, acute follicular conjunctivitis, epidemic keratoconjunctivitis, cystitis and gastroenteritis. (biologydiscussion.com)

Genetic analysis of adenovirus type 2. (microbiologyresearch.org)
The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA. (uni-bielefeld.de)
It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)
In rats, human adenovirus type 9 (Ad9) elicits mammary fibroadenomas that are similar to common breast tumors in women, as well as phyllodes-like tumors and mammary sarcomas. (princeton.edu)
Cell-specific delivery of a transforming growth factor-beta type I receptor kinase inhibitor to proximal tubular cells for the treatment of renal fibrosis. (rug.nl)
An E1/E3 deleted adenovirus type 5 vector expressing cre recombinase under CMV promoter control. (umich.edu)
E1/E3 deleted, CMV promoter, expressing human p53 wild type. (umich.edu)

IMPORTANCE Infections with the human cytomegalovirus (HCMV) may cause severe and even life-threatening disease manifestations in newborns and immunosuppressed individuals. (asm.org)
We show that focused ion beam/scanning electron microscopy (FIB/SEM) tomography is an excellent method to analyze the three-dimensional structure of a fibroblast nucleus infected with human cytomegalovirus (HCMV). (shengsci.com)

It is estimated that up to 20% of all types of human cancers worldwide are attributed to viruses. (ac.ir)
p16 is a cyclin-dependent kinase inhibitor that has shown prognostic utility in some human cancers. (umich.edu)
Transforming function of the LSM1 oncogene in human breast cancers with the 8p11-12 amplicon. (musc.edu)

Genetic analysis of indefinite division in human cells: identification of four complementation groups. (cellosaurus.org)
Genetic characteristics of the human hepatic stellate cell line LX-2. (cellosaurus.org)
Identifying and validating causal genetic alterations in human breast cancer. (musc.edu)

Aim: In this study, we examined whether adenovirus-mediated overexpression of BMP-7 (Ad-BMP-7) antagonised the effect of TGFβ in vitro and in vivo. (mssm.edu)

In addition, a few types of human adenoviruses induce undifferentiated sarcomas in newborn hamsters and other rodents and can transform certain rodent and human cell cultures. (biologydiscussion.com)

Adenovirus 5 E1A is responsible for increased expression of insulin receptor substrate 4 in established adenovirus 5-transformed cell lines and interacts with IRS components activating the PI3 kinase/Akt signalling pathway. (ox.ac.uk)
HPV16-E7 expression causes fluorodeoxyuridine-mediated radiosensitization in SW620 human colon cancer cells. (musc.edu)
erbB family receptor expression and growth regulation in a newly isolated human breast cancer cell line. (musc.edu)
Genomic and expression analysis of the 8p11-12 amplicon in human breast cancer cell lines. (musc.edu)

Transmissible spongiform encephalopathy (TSE) characterized by the accumulation in the central nervous agents have contaminated human tissue-derived medical system and, less often, in lymphoid tissues of TSE- products, human blood components, and animal vaccines. (cdc.gov)
It concerns vaccines containing live viruses which have been prepared from human cell lines of foetal origin, using tissues from aborted human foetuses as a source of such cells. (resistbiden.org)

In this regard, the AstraZeneca (AZ) vaccine has a slight advantage over the Johnson & Johnson (J&J) vaccine because the virus normally infects chimpanzees rather than humans, so fewer people are likely to have been exposed to it. (theepochtimes.com)
Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)

Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)
Mutations of p53 are associated with many forms of human malignancy. (umich.edu)

Viruses and human cancer. (ac.ir)
because viruses could be good vehicles to transport good genes into the human cell. (kaloramainformation.com)
The other important viruses of this group are JC virus (associated with neurological degeneration), BX virus (which suppresses immune system of humans), K virus of mice, etc. (biologydiscussion.com)

Evidence for the involvement of sFlt1 in the occurrence of PE was initially provided by an animal model in which gravid rats were infected with a recombinant adenovirus encoding sFlt1 and compared to animals infected with the empty control adenovirus. (hindawi.com)

4. Mesri EA, Feitelson MA, Munger K. Human viral oncogenesis: a cancer hallmarks analysis. (ac.ir)
It has a role in inhibiting cell proliferation, with prognostic and therapeutic implications for human cancer. (umich.edu)
Cancer has been in existence longer than human beings, and man has been facing the illness ever since he made his appearance on Earth. (jcancer.org)
The first description of human cancer can be found in the Edwin Smith Papyrus dated 3000 BC that illustrated a case of breast cancer. (jcancer.org)
Multiple interacting oncogenes on the 8p11-p12 amplicon in human breast cancer. (musc.edu)
p38MAPK-activated AKT in HER-2 overexpressing human breast cancer cells acts as an EGF-independent survival signal. (musc.edu)
Transforming properties of 8p11-12 amplified genes in human breast cancer. (musc.edu)
Identification and functional analysis of 9p24 amplified genes in human breast cancer. (musc.edu)

293T (or HEK 293T) is a derivative human cell line that expresses a mutant version of the SV40 large T antigen. (wikipedia.org)
I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. (cshlpress.com)

Consistent with a direct role in mammary gland oncogenesis, the Ad9 E4 region also exhibited transforming properties in vitro. (princeton.edu)
Induction of metabolism and transport in human intestine: Validation of precision-cut slices as a tool to study induction of drug metabolism in human intestine in vitro. (rug.nl)

By constructing recombinant adenoviruses between Ad9 and Ad26 (a related nontumorigenic virus), it was shown that the Ad9 E4 region was absolutely required to produce these mammary tumors. (princeton.edu)
A bcl-xS adenovirus selectively induces apoptosis in transformed cells compared to normal mammary cells. (musc.edu)

These findings are discussed in relation to the role of pVI in triggering the adenovirus maturation pathway. (microbiologyresearch.org)

It's not just human sewage either - the fecal matter of livestock and other animals ends up in the groundwater as well. (tyentusa.com)
The colon then absorbs additional fluid, transforming a relatively liquid fecal stream in the cecum to well-formed solid stool in the rectosigmoid. (medscape.com)

Coordinated induction of drug transporters and phase I and II metabolism in human liver slices. (rug.nl)

Similarly, sEng is a truncated form of receptor for two subtypes of transforming growth factor beta (TGF β ) specifically, TGF β 1 and TGF β 2 which are highly expressed by vascular endothelial cells and syncytiotrophoblasts. (hindawi.com)

E1 and partial E3 deleted adenoviral vector, RSV LTR promoter, and expresses human Bclx short (BclxS). (umich.edu)

The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutant. (microbiologyresearch.org)
Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
We describe a method for generating transformed B cell lines using Epstein-Barr virus. (jove.com)
Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. (cshl.edu)
Multi-site validation of an amplicon-based sequencing approach for human monkeypox virus. (cdc.gov)

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