Transfer of nucleic acids transfection. Medical search. Frequent questions

A major advantage of the method is the mild treatment of the cells in comparison to liposome-based transfection reagents (lipofection) and electroporation, which may result in the death of 20-50% of cells. (wikipedia.org)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
The Transfection Reagents And Equipment Market is expected to witness substantial growth in the future due to the rising number of cancer patients worldwide. (verifiedmarketresearch.com)
The Global Transfection Reagents And Equipment Market report provides a holistic evaluation of the market. (verifiedmarketresearch.com)
Cationic lipids and/or polymers are frequently used in transfection reagents, which interact with negatively charged nucleic acids to facilitate endocytosis and cell entrance. (verifiedmarketresearch.com)
Reagents for transfection are a component of innovative biotechnological procedures. (verifiedmarketresearch.com)
The introduction of novel reagents to expand transfection usage across various applications is anticipated to drive market growth. (verifiedmarketresearch.com)
Early diagnosis and rising clinical trials in developing countries have boosted the use of transfection reagents and equipment. (verifiedmarketresearch.com)
The region is on the pathway to becoming one of the major players in the global pharmaceutical market and the current funding by central governments will boost the demand for transfection reagents and equipment as organizations work towards innovation in the gene therapeutics marketplace. (verifiedmarketresearch.com)
Also, transfection reagents are very expensive which limits their use for many applications. (verifiedmarketresearch.com)
The Global Transfection Reagents And Equipment Market is Segmented on the Basis of Product, End User, Method, Application, And Geography. (verifiedmarketresearch.com)
We form cationic liposomes as gene transfer reagents, and we can easily introduce genes into cells, including HilyMax, nucleic acid extraction reagents, nucleic acid staining reagents, electrophoresis reagents, and DNase and RNase that can be used for molecular biology applications. (dojindo.com)

The transfection process is different from "infection," which is the term used for a viral method of introducing nucleic acids into cells (Promega). (newworldencyclopedia.org)

The binding of the negatively charged nucleic acids to the positively charged iron particles occurs relatively fast. (wikipedia.org)

Regardless of the transfection method, osmolality is important when transfecting cells with plasmid to initiate viral production. (labmanager.com)
In conjugation, genetic material is exchanged during a temporary union between two cells, which may entail the transfer of a plasmid or transposon. (denguedenguedengue.com)
In genetics or molecular genetics, the present method is mostly used for transferring a gene, DNA sequence, unknown nucleic acid, viral DNA or plasmid DNA in any cell. (denguedenguedengue.com)
These transfection complexes must subsequently break out from the endosome and transfer the nucleic acid to the cytoplasm (mRNA, siRNA) or the nucleus in order to prevent intracellular breakdown (plasmid DNA). (verifiedmarketresearch.com)
Cell-free assays and in vitro transfections highlighted the versatility of this system with small quantities of T7 RNAP mRNA required to mediate expression at levels that were significantly greater than with the T7-driven plasmid alone or supplemented with T7 RNAP protein. (ox.ac.uk)
The results from this study demonstrate that co-delivery of mRNA is a promising strategy to yield increased expression with plasmid DNA, and represents an important step towards improving the capability of non-viral vectors to mediate efficient gene transfer in cell types, such as in DRGN, where the nuclear membrane is a significant barrier to transfection. (ox.ac.uk)
In this study a rapid test procedure based on intergeneric conjugal plasmid transfer that permitted the distinction between restriction-negative and restriction-positive C. glutamicum clones was developed. (uni-bielefeld.de)
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses an innovative technology developed by Macherey-Nagel to reduce the level of endotoxins copurified during plasmid preparations from bacterial lysates. (takarabio.com)
The NucleoSpin Plasmid Transfection-grade kit yields a plasmid solution containing sufficiently low endotoxin levels for direct transfection into common cell lines. (takarabio.com)
A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH und Co. KG, Bielefeld, Germany) was purified from E. coli using the NucleoSpin Plasmid Transfection-grade kit. (takarabio.com)
The similar ratios observed indicated that cell survival is not affected by addition of DNA eluates purified with the NucleoSpin Plasmid Transfection-grade kit. (takarabio.com)
The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify up to 1mg of plasmid DNA from a 250ml bacterial culture. (promega.com)
The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. (promega.com)

Commonly, the term transfection is used in the sense of non-viral introductions of nucleic acid specifically into eukaryotic cells, or even more specifically into animal cells. (newworldencyclopedia.org)
The term transformation often is preferred to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells such as fungi , algae , and plants . (newworldencyclopedia.org)
However, transformation also is used in other senses as well, including to the non-viral nucleic acid uptake specifically in bacteria and non-animal eukaryotic cells such as fungi , algae , and plants . (newworldencyclopedia.org)
Transformation of eukaryotic cells in tissue culture is usually called transfection. (newworldencyclopedia.org)
Transfection: it's a process of introducing nucleic acid into live eukaryotic cells. (denguedenguedengue.com)
The act of intentionally introducing unprocessed or refined nucleic acids into eukaryotic cells is known as transfection. (verifiedmarketresearch.com)

Magnet-assisted transfection is a transfection method which uses magnetic interactions to deliver DNA into target cells. (wikipedia.org)
Nucleic acids are associated with magnetic nanoparticles, and magnetic fields drive the nucleic acid-particle complexes into target cells, where the nucleic acids are released. (wikipedia.org)
In contrast, methods like lipofection offer only statistical hits between cargo and cells, because of the three-dimensional motion of cells and transfection aggregates in a liquid suspension. (wikipedia.org)
Broadly defined, transfection is the process of artificially introducing nucleic acids ( DNA or RNA ) into cells , utilizing means other than viral infection. (newworldencyclopedia.org)
Transfection of animal cells typically involves opening transient pores or "holes" in the cell plasma membrane , to allow the uptake of material. (newworldencyclopedia.org)
Originally, the term was used for the artificial insertion of viral nucleic acid into bacterial cells, resulting in multiplication of mature viral particles (Dorland 2007). (newworldencyclopedia.org)
Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of nucleic acids by non-viral methods. (newworldencyclopedia.org)
1990) state that the meaning of transfection is "clear and unambiguous" and refers to the "process by which nucleic acid (DNA or RNA) is introduced to cells with the intent to be expressed in the recipient. (newworldencyclopedia.org)
Since the DNA introduced in the transfection process is usually not inserted into the nuclear genome, the foreign DNA is lost at the later stage when the cells undergo mitosis . (newworldencyclopedia.org)
If it is desired that the transfected gene actually remains in the genome of the cell and its daughter cells, a stable transfection must occur. (newworldencyclopedia.org)
Here we describe transient protein production using QMCF Technology (Icosagen, Estonia) that uses specific suspension-adapted mammalian cell lines (QMCF cells), appropriate QMCF episomal expression vector, a chemically defined animal origin-free, serum-free medium, and Reagent 007™ (Icosagen, Estonia) for efficient delivery of nucleic acids for transfection of mammalian cells in comparison with PEI MAX™ (Transfection Grade Linear Polyethylenimine Hydrochloride, Polysciences). (springer.com)
Karro K, Männik T, Männik A, Ustav M (2015) DNA transfer into animal cells using stearylated CPP based transfection reagent. (springer.com)
HEK293 cells, which are highly transfectable, commonly undergo polyethyenimine (PEI)-mediated transfections due to the known effectiveness. (labmanager.com)
Additionally, the invention provides methods of making the compositions, as well as methods of introducing the nucleic acids into cells using the compositions and treating disease conditions. (justia.com)
To produce a GM plant, new DNA is transferred into plant cells. (denguedenguedengue.com)
What are the various methods of gene transfer in plant cells? (denguedenguedengue.com)
Apply electrical pulse to cells in the presence of specialized buffer and nucleic acids. (denguedenguedengue.com)
This method is versatile due to the fact that it can be successfully used for the DNA transfer in mammalian cells and microorganisms. (denguedenguedengue.com)
For the effective delivery of DNA, siRNA, oligos, and RNA into adherent and suspension cells, particularly difficult-to-transfect cells, there is a significant demand for transfection solutions. (verifiedmarketresearch.com)
However, the transfection of cells is usually associated with cellular stress which increases cell toxicity. (verifiedmarketresearch.com)
In this system, CS serves to encapsulate and deliver DNA cargo to intestinal cells in the form of CS-DNA nanoparticles (CS-DNA NPs), while ZN is used to form a protective matrix around the CS-DNA NPs that prevent degradation during gastric transit but then degrades to release the CS-DNA NPs for transfection upon entry into the intestines. (bvsalud.org)
Gene therapy is extensively studied as a realistic and promising therapeutic approach for treating inherited and acquired diseases by repairing defective genes through introducing (transfection) the "healthy" genetic material in the diseased cells. (bvsalud.org)
transfection of suspension-growing cells was more efficient with linear PEI and was not affected by the presence of medium conditioned for 24 h. (canada.ca)
Sonoporation, thus, employs acoustic cavitation of microbubbles interacting with nearby cell membrane s that consequently enables the transfer of macromolecules into the recipient cells. (biologyonline.com)
1 When employing sonoporation for transfection or transformation of cells, cellular viability must be considered because prolonged exposure to low-frequency ultrasound may result in the rupturing, and ultimately to the death, of the cell. (biologyonline.com)
We have developed a line of HappyFect products, which can be used to improve the delivery of a range of molecules into cells, including nucleic acids. (hum-molgen.org)
For the transfer of whole chromosomes, metaphase cells are selected. (mcqexams.com)
The fragments of the chromosomes are incubated with whole cells/ eggs for transfection. (mcqexams.com)
Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication due to their ability to transfer bioactive lipids, proteins and different species of RNA into cells. (selectbiosciences.com)
The resistance transfer capacity was determined by flow cytometry after sensitive cells incubated 48 hours with exosomes from drug-resistant cells. (esmo.org)
Exosomes from chemotherapy-resistant TNBC cells could transfer drug resistance to sensitive cells via exosomal miRNAs. (esmo.org)
Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)

In addition to electroporation, transfection can be carried out using calcium phosphate, or by mixing a cationic lipid with the material to produce liposomes, which fuse with the cell plasma membrane and deposit their cargo inside. (newworldencyclopedia.org)
What is electroporation method of gene transfer? (denguedenguedengue.com)
Thus, electroporation is employed for in vitro transfection experiments. (denguedenguedengue.com)
What are the disadvantages of electroporation and transfection? (denguedenguedengue.com)
Electroporation can transfer foreign material effectively, faster than any other viral or non-viral vector-mediated delivery system. (denguedenguedengue.com)

Trans IT™-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production. (fishersci.com)
The Effectene Transfection Reagent is intended for molecular biology applications. (qiagen.com)
Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. (qiagen.com)
Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. (qiagen.com)
In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. (qiagen.com)

Magnet-assisted transfection is a relatively new and time-saving method to introduce nucleic acids into a target cell with increased efficiency. (wikipedia.org)
citation needed] In addition, the transfection efficiency is increased in numerous cases by the directed transport in a magnetic field, especially for low amounts of nucleic acids. (wikipedia.org)
Additionally, synergistic effects in transfection efficiency can arise from the possible combination of lipofection and magnet-assisted transfection. (wikipedia.org)
Achieving high transfection efficiency is critical for high viral titer yield, but it is common to see subpar transfection efficiencies in gene therapy development. (labmanager.com)
To succeed, the proper DNA or RNA fragments need efficient vectors, and viruses are endowed with excellent transfection efficiency and have been extensively exploited. (bvsalud.org)
Green fluorescent protein (GFP) and human placental secreted alkaline phosphatase (SEAP) were used as reporter genes to monitor transfection efficiency and productivity. (canada.ca)
We have also characterized a number of parameters, such as mRNA structure, intracellular stability and persistence of each nucleic acid component that represent important factors in determining the transfection efficiency of this hybrid expression system. (ox.ac.uk)
The transfection efficiency was evaluated by qRT-PCR and Western blotting. (docksci.com)
There was maximum transfection efficiency and no significant increase in toxicity at 15 pmol/L. Bmi-1 and hTERT expression decreased, but the inhibition rate increased in the hTERT siRNA group, the hTERT+Bmi-1 siRNA group and the hTERT+Bmi-1 siRNA group compared with the scrambled siRNA group and the control group. (docksci.com)
To solve the problems of low siRNA transfection efficiency and degradation by nucleases, we used Lipid-polycation-nucleic acid (LPN) complex nanocarrier vectors. (docksci.com)

295 "Gene escape model: transfer of heavy metal resis -tance genes from E.coli":App. (globalchange.com)
How do genes transfer from one plant to another? (denguedenguedengue.com)
How genes are transferred? (denguedenguedengue.com)
Pol II, assisted by the neurodegeneration-associated enzyme senataxin, generates a shield comprising triplex nucleic acid structures known as R-loops at intergenic spacers flanking nucleolar rRNA genes. (nature.com)

In future, this technology might be also an alternative strategy to the currently used viral and non-viral vectors in gene-therapy and gene transfer. (wikipedia.org)
Arukuusk P, Pärnaste L, Oskolkov N et al (2013) New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids. (springer.com)
The complex, composed of Lipo, PEI and siRNA, is low toxicity and efficient transfection vectors. (docksci.com)

Transfection of SLC7A11 siRNA effectively abrogated the increase in chondrocyte viability induced by the miR-181 inhibitor and increased ferroptosis. (biomedcentral.com)

In transfection, the introduced nucleic acid may be transient, such that it does not replicate, or it may be stable and integrate into the genome of the recipient and replicate when the host genome replicates. (newworldencyclopedia.org)
Longo PA, Kavran JM, Kim M-S, Leahy DJ (2013) Transient mammalian cell transfection with polyethylenimine (PEI). (springer.com)

Particles and nucleic acids form complexes by ionic interaction of the negatively charged nucleic acid and the positively charged surface of the magnetic nanoparticle. (wikipedia.org)

Transfection of mRNAs encoding the chimeric constructs achieved functional expression of the proteins able to act on their targets. (unav.edu)
Transfection of a miR-181b inhibitor could increase the expression levels of the ferroptosis-related proteins solute carrier family 7 members 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), thereby inhibiting the occurrence of ferroptosis in chondrocytes. (biomedcentral.com)

We have developed and evaluated a hybrid mRNA/DNA system designed to bypass the nuclear barrier to transfection and facilitate cytoplasmic gene expression. (ox.ac.uk)

Magnet-assisted transfection can also be performed in the presence of serum, which is a further benefit. (wikipedia.org)
The presence of serum had a positive effect on gene transfer and expression. (canada.ca)

1. A purified protease which comprises the amino acid sequence SEQ ID NO:1, or a functional equivalent thereof produced by conservative amino acid alterations which result in a protease which exhibits the same biological, biochemical, chemical, physical and structural properties of the protease of SEQ ID NO:1. (everypatent.com)

Development in the field of recombinant technology & proteomics and the growing usage of transfection technology by academic researchers are some of the key trends influencing the market growth. (verifiedmarketresearch.com)
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. (qiagen.com)

A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension. (canada.ca)

In order to evaluate the regulation, expression or activity of a gene, it is necessary to transfer the gene or its manipulated form into the in vitro systems. (benthamscience.com)

Transfection also has been used to refer to infecting a cell specifically with viral nucleic acid that is either isolated from a eukaryote virus or from a bacteriophage . (newworldencyclopedia.org)

Definition: "Using the electrical current any biological molecules such as nucleic acid, drug, chemical or viral DNA can be inserted into a live cell by creating temporary pores in the cell membrane. (denguedenguedengue.com)

The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)

Paraffin-embedded glioma specimens were used for MiRNA locked nucleic acid (LNA) in situ hybridization and immunohistochemistry, and frozen glioma specimen were used for Real-time PCR. (biomedcentral.com)
Locked nucleic acid probes and enzyme-labeled fluorescence (LNA-ELF-FISH) was performed to detect exosomal miRNA molecule transfer. (esmo.org)
Exosomal miRNA molecule transfer was detected using LNA-ELF-FISH. (esmo.org)

Kunitz-like domain"--A protease inhibitor similar to soybean trypsin inhibitor or a nucleic acid sequence encoding a protease inhibitor which is similar to the soybean trypsin inhibitor. (everypatent.com)
vi)a nucleic acid sequence according to any of (i) and (ii) which is degenerate as a result of the genetic code. (allindianpatents.com)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. (justia.com)

RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. (uni-bielefeld.de)

These included 'tagging' cellular compounds with flourescent antibodies or with the enzyme horseradish peroxidase, or using nucleic acid probes which bind to cellular DNA. (nih.gov)

Extracted nucleic acid and specimens that may contain only OPV (i.e. (cdc.gov)

The magnetic nanoparticles have an approximate size of 100 nm and are additionally coated with biological polymers to allow loading of nucleic acids. (wikipedia.org)

To investigate the occurrence, development and transfer process of MM, a novel molecular targeted therapy was established to provide a reliable basis for research. (spandidos-publications.com)

260 "The polymerase chain reaction: an improved method for the analysis of nucleic acids":Hum.Genet. (globalchange.com)
What is the most effective method for gene transfer in plants? (denguedenguedengue.com)
Particle (or micro projectile) bombardment is the most effective method for gene transfer, and creation of transgenic plants. (denguedenguedengue.com)
The aim of the present study was to assess the effects of sprouty homolog 2 (SPRY2) gene regulation by miR-21 on the occurrence, development and tumor metastasis in multiple myeloma (MM). The miR‑21 expression lentiviral vector (LV)‑anti‑miR‑21 and a liposome transfection method were used to screen MM cell lines with stable silent SPRY2. (spandidos-publications.com)

The device eliminates the need for a centrifuge with a swinging bucket rotor for nucleic acid purification. (promega.com)

We develop a multifocal HCC model to test immunotherapies by introducing c-myc using hydrodynamic gene transfer along with CRISPR-Cas9-mediated disruption of p53 in mouse hepatocytes. (unav.edu)

In the following chapter, we will discuss the advantages and disadvantages of different methods of transfection. (benthamscience.com)

Manipulating with nucleic acid is a trend in biotechnology. (mcqexams.com)

Methods for the preparation of a lipid-nucleic acid composition are provided. (justia.com)

This invention relates to compositions comprising a combination of a lipid and a therapeutic agent, particularly to lipid-nucleic acid compositions, for in vivo therapeutic use. (justia.com)

Magnetically enhanced nucleic acid delivery. (wikipedia.org)

In particular, adherent mammalian cell lines and primary cell cultures show very high transfection rates. (wikipedia.org)

There are many methods employed in performing transfection. (newworldencyclopedia.org)
This article throws light upon the two methods used for gene transfer in plants. (denguedenguedengue.com)
The two methods are: (1) Vector-Mediated Gene Transfer and (2) Direct or Vector-less DNA Transfer. (denguedenguedengue.com)

A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. (promega.com)

For most applications of transfection, it is sufficient if the transfected gene is only transiently expressed. (newworldencyclopedia.org)
Tecrea is developing products that are uniquely non-toxic and effective for your transfection applications. (hum-molgen.org)

2001. Evaluation of benzene exposure in children living in Campania (Italy) by urinary trans,trans -muconic acid assay. (cdc.gov)

Anatomy16

Organisms15

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Psychiatry and Psychology1

Phenomena and Processes82

Disciplines and Occupations4

Technology, Industry, Agriculture8

Information Science7

Health Care3

Reagents12

Introducing nucleic acids into cells1

Negatively charged nucleic acids1

Plasmid13

Eukaryotic cells6

Cells33

Electroporation5

Reagent5

Efficiency10

Genes4

Vectors3

SiRNA1

Transient2

Complexes1

Proteins2

MRNA1

Presence of serum2

Amino acid seq1

Recombinant2

Polyethylenimine1

Vitro1

Bacteriophage1

Molecules1

Polypeptide1

MiRNA3

Sequence3

Particles1

Conjugation1

Probes1

Specimens1

Polymers1

Targeted therapy1

Method4

Purification1

Hydrodynamic1

Disadvantages1

Biotechnology1

Composition1

Combination1

Delivery1

Cell lines1

Methods3

Tube1

Applications2

Assay1