AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
TransfectionPolyethyleneimineGene Transfer TechniquesLiposomesPlasmidsCationsPolylysineDNAElectroporationParticle SizeGenetic TherapyGenetic VectorsLuciferasesQuaternary Ammonium CompoundsNanoparticlesCation Exchange ResinsChitosanPhosphatidylethanolaminesFatty Acids, MonounsaturatedDrug CarriersPolymersGreen Fluorescent ProteinsNanocapsulesCell SurvivalCell Linebeta-GalactosidaseCell Line, TumorUltrasonicsGenes, ReporterPolyethylene GlycolsLipidsDendrimersNylonsGene ExpressionCOS CellsMicrobubblesPoloxamerSurface-Active AgentsProtaminesRNA, Small InterferingPolyethylenesCells, CulturedGlycerophospholipidsMolecular Sequence DataBase SequenceAdenoviridaeTransgenesHeLa CellsIminesNanostructuresPhosphoric AcidsMagnetic FieldsPolyglycolic AcidFlow CytometryCercopithecus aethiopsCricetinaePromoter Regions, GeneticMethacrylatesTumor Cells, CulturedNIH 3T3 CellsPolyaminesLuminescent ProteinsCHO CellsBuffersPolymethacrylic AcidsStatic ElectricityRNA, MessengerDrug Delivery SystemsMagnetite NanoparticlesMicroscopy, ConfocalSpermineAbsorbable ImplantsHEK293 CellsElectrophoresis, Agar GelScattering, RadiationElectrophoretic Mobility Shift AssayMicroscopy, FluorescenceBiocompatible Materials3T3 CellsIndicators and ReagentsGene Expression RegulationRecombinant Fusion ProteinsChloramphenicol O-AcetyltransferaseMice, NudeEndosomesPolyestersDNA, RecombinantPeptidesRecombinant ProteinsRNA InterferenceLac OperonTime FactorsTransferrinGene SilencingMice, Inbred BALB CGene TargetingFluorescent DyesReverse Transcriptase Polymerase Chain ReactionMolecular StructureCricetulus
ReagentRNAiHighly efficient transfectionMammalianVitroRISCTransient transfectionVivoSiRNAsDetermine transfection efficiencyVectorDisease causiCytotoxicityOverexpressionUptakeEukaryotic cellsKnockdown of targetVectorsTargetsElectroporationCellsMiRNAPost-transcriptional levelShRNAEfficientMoleculesSignaGen LaboratoriesAntibodyToxicityPromoterStablyRecombinantApoptosisMRNA and proteinEfficacyDeliveryExperimentalCellularCell proliferationMutationsHighSignificantlyExperimentsReceptorSequencesDharmaFECTInterferenceCas9Target
Reagent22
  • [ 46 ] Recently Jiao and colleagues used PepMute™ (SignaGen Laboratories, MD, USA) siRNA Transfection Reagent, a formulation of simulated virus cell penetrating peptides, for efficient delivery of siRNA in human embryonic kidney cells. (medscape.com)
  • This novel siRNA delivery reagent facilitated effective down regulation of influenza A/environment/Qinghai/1/2008(H5N1) NS1 gene expression as observed by the reduced mRNA and protein levels in HEK293 cell. (medscape.com)
  • The HiPerFect Transfection Reagent is intended for molecular biology applications. (qiagen.com)
  • HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. (qiagen.com)
  • In addition to siRNA, HiPerFect Transfection Reagent is ideally suited to transfection of miRNA mimics or inhibitors. (qiagen.com)
  • Cell-type-specific protocols using HiPerFect Transfection Reagent are available at the TransFect Protocol Database. (qiagen.com)
  • A range of suspension cell lines and differentiated and undifferentiated macrophages have been tested using HiPerFect Reagent for siRNA transfection. (qiagen.com)
  • Transfection with HiPerFect Reagent is a superior alternative to electroporation for many of these cell types. (qiagen.com)
  • Some cell lines were not amenable to transfection using HiPerFect Reagent (see Table 2). (qiagen.com)
  • Using HiPerFect Transfection Reagent, highly efficient transfection and silencing have been observed, in some cases with as little as 10 pM siRNA. (qiagen.com)
  • HiPerFect Transfection Reagent provides highly efficient siRNA transfection over a range of siRNA concentrations from low to high, allowing researchers to choose the siRNA concentration they wish to use. (qiagen.com)
  • Reagent exhibits at least 79% transfection efficiency of siRNA delivery. (altogen.com)
  • Using a high-efficiency transfection reagent provides a higher degree of experimental reproducibility and low cytotoxicity. (altogen.com)
  • Altogen Biosystems synthesizes biodegradable polymer-based high-efficiency transfection reagent kits for the BALB/3T3 mouse embryo fibroblast cell line. (altogen.com)
  • For transfection procedure, follow protocol provided by the transfection reagent manufacturer. (cellsignal.com)
  • Silencing efficiency comparison of PepMute Transfection Reagent (upper panel) with Dharmafect 4 (middle panel) and Lipofectamin e RNAiMAX (RNAiMAX, lower panel) siRNA Transfection Reagents on A549 cells. (signagen.com)
  • PepMute Transfection Reagent knocked down stable GFP expression in MCF7 cell (upper panel) and U2OS cell (lower panel) by reversely transfecting 5.0 and 1.0 nM GFP siRNA respectively. (signagen.com)
  • In contrast treatment with siRNA alone or with a conventional transfection reagent PEI (polyethylenimine) showed negligible silencing effects (Number 1d) as did graded concentrations of siCD4 encapsulated in isotype control mAb-coated nanoparticles (IgG-NPs) (Number 1c). (healthyguide.info)
  • You have access to all the documents related to the transfection reagent. (polyplus-sartorius.com)
  • TurboFectin 8.0 is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. (origene.com)
  • TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone/TrueORF Clone (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many different cell types. (origene.com)
  • Preferred reagent for reverse transfection assays. (origene.com)
RNAi27
  • Depending on the purpose of the RNAi experiment, the optimal concentration of siRNA to use may be 1 nM (minimal risk of off-target effects and efficient knockdown) or 5 nM (higher knockdown efficiency). (qiagen.com)
  • A ready-to-use reverse transfection format RNAi screening library targeting mouse cytokine receptors. (horizondiscovery.com)
  • Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, similar to miRNA, and operating within the RNA interference (RNAi) pathway. (wikipedia.org)
  • In 1998, Andrew Fire at Carnegie Institution for Science in Washington DC and Craig Mello at University of Massachusetts in Worcester discovered the RNAi mechanism while working on the gene expression in the nematode, Caenorhabditis elegans. (wikipedia.org)
  • Compared to RNA interference (RNAi), CRISPR/Cas9-mediate gene KO has the advantage of creating true genetic nulls instead of hypomorphs. (biomedcentral.com)
  • On the other hand, RNAi, particularly in the form of siRNA transfection, is easy to use due to the simplicity in its delivery. (biomedcentral.com)
  • Because RNAi utilizes the endogenous RNA-induced silencing complex (RISC), gene knockdown can be achieved by simply transfecting a synthetic short-interfering RNA (siRNA) duplex into cells, a process that is both highly efficient and easily scalable. (biomedcentral.com)
  • RNA interference (RNAi) has been regarded as a vital asset in the field of therapeutics as it has the capability to silence various disease causing genes including those that cause cancer. (nih.gov)
  • Small non-coding RNA molecules such as short interfering RNAs (siRNAs) are one of the extensively studied RNAi inducers for gene modulations. (nih.gov)
  • As a naturally occurring post-transcriptional gene regulatory mechanism, RNAi is currently a very promising therapy due to its precise multi-targeted gene silencing. (bocsci.com)
  • RNA interference(RNAi) is a process involving sequence-specific gene silencing, which mainly involves endogenous non-coding RNA such as microRNA(miRNA), small interfering RNA(siRNA), long non-coding RNA(lncRNA), and cyclic RNA(circRNA) to target and silence messenger RNA(mRNA) in a sequence-specific manner. (bocsci.com)
  • RNAi has the added advantage of targeting multiple genes with homology at the same time, quickly, economically, and with the potential for site-specific targeting. (bocsci.com)
  • To be more specific, the primary role of RNAi in cells is to down-regulate the expression of target genes, which in turn down-regulates the expression of the encoded proteins, leading to the desired results. (bocsci.com)
  • instability of RNA molecules, low transfection efficiency and half-life, lack of site-specific targeting and distribution in target tissues, and tumor heterogeneity add to the challenges of gene silencing using the RNAi process. (bocsci.com)
  • RNA interference (RNAi)-mediated knockdown of gene expression offers a book treatment technique for individual immunodeficiency AZD2281 trojan (HIV) infection. (healthyguide.info)
  • Launch Sequence-specific gene silencing by RNA disturbance (RNAi) has been explored being a book therapeutic strategy in a number of illnesses.1 Regardless of the option of highly dynamic antiretroviral therapy there's a particularly solid curiosity about developing RNAi being a therapeutic choice for individual immunodeficiency trojan (HIV) due to the significant practical complications such as for example toxicity and advancement of drug level of resistance connected with lifelong treatment. (healthyguide.info)
  • Different from other gene silencing phenomena (such as TGS, PTGS), RNAi has highly significant efficiency, specificity, and transferability. (atagenix.com)
  • Flexible applications of RNAi can specifically knock out or inhibit the expression of target genes. (atagenix.com)
  • 1. Determine the target gene, design and synthesis of RNAi, construct the vector for transfection. (atagenix.com)
  • For stable transfection RNAi, AtaGenix provides 1-3 stable cell lines, attached with lab reports (the same as transient transfection of RNAi). (atagenix.com)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • Herein, we investigated whether RNAi-interfering nanoparticles could deliver siRNA to NSCLC cells and silence PLK1 expression in vitro and in vivo . (oncotarget.com)
  • RNAi is a naturally occurring form of post-transcriptional gene silencing (PTGS) mediated by double-stranded RNA (dsRNA). (nih.gov)
  • By taking advantage of RNAi mechanisms, researchers have been able to reduce the expression of specific target genes on an individual cell to organism-wide scale. (nih.gov)
  • It was shown that the introduction into embryos of antisense (but, unexpectedly, also sense) dsRNA induced a sequence-specific gene silencing at a post-transcriptional level, an effect that was termed RNAi. (nih.gov)
  • Having found RNAi gene silencing in worms and flies, investigators now turned their attention to determining whether RNAi could be induced in mammalian cells. (nih.gov)
  • It was known, however, that in somatic mammalian cells, the introduction of long dsRNAs (~70+ nucleotides) led to nonspecific suppression of gene expression instead of the sequence-specific gene silencing seen in RNAi. (nih.gov)
Highly efficient transfection3
  • Over 300 chemicals have been synthesized and several of them showed highly efficient transfection in different cell types, surpassing the efficiency of several popular commercial transfection reagents. (screenfect.com)
  • Positive dielectrophoresis (pDEP) was used to selectively place cells on the nanochannel outputs, thus allowing for controlled and highly efficient transfection. (confex.com)
  • Our regular plasmid Tet regulatory protein expression vector allows highly efficient, transfection-based delivery of Tet regulatory protein(s) into mammalian cells. (vectorbuilder.cn)
Mammalian11
  • In 2001, the expression of a specific gene was successfully silenced by introducing chemically synthesized siRNA into mammalian cells (Tuschl et al. (wikipedia.org)
  • Our method is a convenient yet powerful tool to enable rapid and scalable gene knockout using CRISPR/Cas9 in mammalian cells. (biomedcentral.com)
  • Gene paralog redundancy is a common phenomenon in the mammalian genome. (biomedcentral.com)
  • With our proprietary peptide simulation technology (PST), hundreds of viral CPPs were simulated, synthesized and screened for gene delivery efficacy in variety of mammalian cells (Figure 1). (signagen.com)
  • The Lentivirus IPTG-Inducible shRNA Knockdown vector is a highly efficient system for achieving temporal knockdown of target genes in a wide variety of mammalian cell lines and offers an effective tool for studying genes essential for cell development or cell survival. (vectorbuilder.kr)
  • The RNA-induced silencing complex (RISC) pathway in mammalian cells. (idtdna.com)
  • Choose among a broad range of powerful transfection reagents specifically developed to enable you to reach the desired level of protein expression in a wide range of adherent and suspension mammalian cell types, primary and cell lines. (polyplus-sartorius.com)
  • VectorBuilder's regular plasmid Tet regulatory protein expression vector can be used for simple transfection-based delivery of Tet regulatory protein(s) (tTS, rtTA, etc.) into mammalian cells to help you achieve tetracycline-inducible expression of target genes placed downstream of a tetracycline responsive-element (TRE) promoter. (vectorbuilder.cn)
  • The Tet-On inducible system is a powerful tool to control the timing of expression of the gene(s) of interest (GOI) in mammalian cells. (vectorbuilder.cn)
  • Delivering plasmid vectors into mammalian cells by conventional transfection is one of the most widely used procedures in biomedical research. (vectorbuilder.cn)
  • We have developed an experimental approach to elucidate the actions of genes that cause neurodegenerative disease in a single mammalian neural circuit. (nih.gov)
Vitro7
  • It has been reported in separate studies, that using lipid-based transfection reagents, about 60% of reduction in target mRNA levels could be achieved in vitro . (medscape.com)
  • The immunoliposomes specifically bound to the HA-expressing Sf9 cells and increased the siRNA transfection efficiency in vitro . (medscape.com)
  • While siRNA has shown promising results in vitro, there are inherent obstacles on the road to achieving gene silencing in vivo and making gene therapy a reality. (bocsci.com)
  • We hypothesize that small interfering RNA (siRNA) targeting MDR1 gene (siMDR1) delivery with microbubble and ultrasound can down-regulate MDR1 expression and improve responsiveness to chemotherapeutic drugs for yolk sac carcinoma in vitro . (biomedcentral.com)
  • In the present study, we established a pancreatic cancer cell line that stably silenced the SnoN gene by siRNA, and investigated the effects of SnoN on the proliferation and apoptosis of pancreatic cancer cells in vitro . (biomedcentral.com)
  • Furthermore, in vitro and in vivo inhibition of G9a could increase the differentiation and proliferation of pacemaker-like cells by decreasing the levels of H3K9me2 in the promoter region of HCN4 gene. (spandidos-publications.com)
  • iNOP-7-PLK1 siRNA silenced PLK1 expression and reduced NSCLC growth in vitro . (oncotarget.com)
RISC5
  • this is what enables the molecules to form the RNA-Induced Silencing Complex (RISC). (wikipedia.org)
  • The nascent siRNA associates with Dicer, TRBP, and Argonaut (Ago2) to form the RNA-induced silencing complex (RISC), which mediates gene silencing (Figure 1) [ 3 ]. (idtdna.com)
  • Once in RISC, one strand of the siRNA (the passenger strand) is degraded or discarded, while the other strand (the guide strand) remains to direct sequence selection of the silencing complex. (idtdna.com)
  • When the homologous dsRNA (double stranded RNA) of the endogenous mRNA are imported into cells under the intracellular enzyme's effect related to the RNA induced silencing complex (RISC), endogenous mRNA degrades, resulting in the silencing of gene expression. (atagenix.com)
  • siRNAs are incorporated into a multiprotein RNA-induced silencing complex, or RISC. (nih.gov)
Transient transfection1
Vivo4
  • siRNAs conjugated to bile acids and long-chain fatty acids mediate siRNA uptake into cells and facilitate effective gene silencing in vivo . (medscape.com)
  • However, further in vivo studies are needed to clarify the influence of SnoN gene silencing by siRNA on pancreatic cancer therapy. (biomedcentral.com)
  • They include a 2D NEP-optical tweezers platform for single cell transfection*, a 3D DEP-NEP platform for large scale cell transfection, and a 3D NEP patch for in vivo gene transfection. (confex.com)
  • The search for an ideal gene delivery system is a long and laborious process in which several factors from the first idea to final formulation, including main challenges, peaks and troughs, should be deeply taken into consideration to ensure adequate biological safety and in vivo efficacy endpoints. (cibbim.eu)
SiRNAs11
  • Transfection of siRNA can result in off-target effects, in which siRNAs affect the expression of non-homologous or partially homologous gene targets. (qiagen.com)
  • siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. (horizondiscovery.com)
  • siGENOME and ON-TARGET plus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA). (horizondiscovery.com)
  • siRNAs can also be introduced into cells by transfection. (wikipedia.org)
  • Since in principle any gene can be knocked down by a synthetic siRNA with a complementary sequence, siRNAs are an important tool for validating gene function and drug targeting in the post-genomic era. (wikipedia.org)
  • siRNAs and their role in post-transcriptional gene silencing(PTGS) was discovered in plants by David Baulcombe's group at the Sainsbury Laboratory in Norwich, England and reported in Science in 1999. (wikipedia.org)
  • miRNAs typically silence genes by repression of translation and have broader specificity of action, while siRNAs typically work with higher specificity by cleaving the mRNA before translation, with 100% complementarity. (wikipedia.org)
  • siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into BALB 3T3 cells following the recommended protocol. (altogen.com)
  • These works indicate that CRISPR/Cas9 can be adapted as a transfection-based method analogous to that use for siRNAs. (biomedcentral.com)
  • Chimeric siRNAs are the preferred strategy to enhance the stability, specificity, or delivery efficiency of siRNA molecules for expanding and optimizing gene therapy applications. (bocsci.com)
  • Chemically modified siRNAs can significantly improve the efficacy of gene therapy by overcoming poor stability, short half-life, and low cellular uptake. (bocsci.com)
Determine transfection efficiency2
Vector13
  • A human immunodeficiency virus (HIV)-1 derived lentivirus vector was used to silence the expression of M1 gene of influenza A/WSN/33 (H1N1) virus in MDCK cells. (medscape.com)
  • 12. siRNA delivery by a transferrin-associated lipid-based vector: a non-viral strategy to mediate gene silencing. (nih.gov)
  • Welgen guarantees that the selected vector will provide 70% silencing efficiency in a co-transfection experiment. (welgen.com)
  • This review provides an update of the new developments in cancer gene therapy, new principles, techniques, strategies and vector systems, and shows how they may be applied in the treatment of gastric cancer. (wjgnet.com)
  • Specifically we will review the current state of science in gene based therapeutics including gene targeted approaches, vector design and delivery options, characteristics of suitable gene molecular targets and mechanisms amenable to gene based therapies. (nih.gov)
  • However, the differentiation efficiency of pacemaker-like cells is still not high and only ~7% of BMSC-Tbx18 cells were reported to beat on day 10 after transfection with a Tbx18 overexpression vector ( 8 ). (spandidos-publications.com)
  • Our lentivirus IPTG-inducible shRNA knockdown vector has been validated for highly efficient target gene knockdown in the presence of IPTG as shown in Figure 1 below. (vectorbuilder.kr)
  • similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. (biomedcentral.com)
  • PEGylated chitosan has been widely used as a gene/drug delivery vector by forming a polyelectrolyte complex (PEC) in biomedical engineering. (sigmaaldrich.com)
  • While our regular plasmid Tet regulatory protein expression vector includes an expression cassette consisting of the Tet regulatory protein(s) driven by a user-selected promoter, the GOI driven by the TRE promoter must be provided using a separate helper vector to achieve tetracycline induced gene expression in the presence of tetracycline, while minimizing leaky expression in the absence of tetracycline. (vectorbuilder.cn)
  • While a number of more sophisticated gene delivery vector systems have been developed over the years such as lentiviral vectors, adenovirus vectors, AAV vectors and piggyBac, conventional plasmid transfection remains the workhorse of gene delivery in many labs. (vectorbuilder.cn)
  • Using this vector to coexpress the Tet regulatory proteins tTS and rtTA along with the TRE driven GOI can achieve nearly complete silencing of the GOI in the absence of tetracycline, and strong, rapid expression in response to the addition of tetracycline. (vectorbuilder.cn)
  • The TALENs were delivered along with a DNA donor vector with a ubiquitous promoter driving expression of a cDNA using a nucleofector to get high transfection efficiencies. (nih.gov)
Disease causi1
  • Short-interfering-RNA (siRNA) holds promise as a class of therapeutics, which can selectively silence disease-causing genes. (oncotarget.com)
Cytotoxicity2
  • 17. Enhanced transfection efficiency and reduced cytotoxicity of novel lipid-polymer hybrid nanoplexes. (nih.gov)
  • This study aims at developing chitosan-based nanoparticles suitable for an intravenous administration of small interfering RNA (siRNA) able to achieve (i) high gene silencing without cytotoxicity and (ii) stability in biological media including blood. (sigmaaldrich.com)
Overexpression1
  • Moreover, the overexpression of MAVS or silencing of miR-3470b by its inhibitors suppressed BEFV replication, and knockdown of MAVS by small interfering RNA also promoted the replication of BEFV. (biomedcentral.com)
Uptake2
  • 16. Enhanced cellular uptake and gene silencing activity of siRNA using temperature-responsive polymer-modified liposome. (nih.gov)
  • Although positively charged mRNA-polyplexes were unsuccessful for NK cell transfection, even when combined with cellular uptake and endosomal escape enhancing peptides, LNPs proved to be a suitable mRNA delivery platform. (uu.nl)
Eukaryotic cells1
  • CRISPR/Cas9 has been adapted for gene knockout (KO) in eukaryotic cells. (biomedcentral.com)
Knockdown of target1
Vectors7
  • Viral vectors, in general exhibit high transfection efficiencies but are expensive and likely to confer immunological and safety issues. (nih.gov)
  • Recent studies have shown that ultrasound microbubble-mediated delivery improves the efficacy of gene transfection and reduces the side effects of other bioactive transfection agents, such as liposome, viral vectors [ 27 ]. (biomedcentral.com)
  • When you design shRNA vectors on VectorBuilder's online platform, you will have the option to search for your target genes in our database. (vectorbuilder.kr)
  • By design, our lentiviral vectors lack the genes required for viral packaging and transduction (these genes are instead carried by helper plasmids used during virus packaging). (vectorbuilder.kr)
  • Gene and Cell Therapy rely on the use of modified viruses as vectors to transfer genes into cells that they are able to infect . (polyplus-sartorius.com)
  • Production of viral vectors is dependent on efficient transfection of one to several plasmid DNA containing the necessary viral genome to produce recombinant viral particles. (polyplus-sartorius.com)
  • Our Tet-On inducible gene expression vectors are designed to achieve nearly complete silencing of a GOI in the absence of tetracycline and its analogs (e.g. doxycycline), and strong, rapid expression in response to the addition of tetracycline or one of its analogs (e.g. doxycycline). (vectorbuilder.cn)
Targets4
  • Short interfering RNA (siRNA) has been widely used to silence its targets through complementary-binding to the target mRNA. (confex.com)
  • Improved molecular biological techniques and a better understanding of gastric carcinogenesis have allowed us to validate a variety of genes as molecular targets for gene therapy. (wjgnet.com)
  • The technology can be widely used to study gene functions, confirm drug targets, gene therapy, etc. (atagenix.com)
  • Generally, microRNAs have emerged as master regulators that typically bind to complementary sequences in the 3′ untranslated region (UTR) of multiple mRNA targets and modulate gene expression either via the translational inhibition or degradation of their target messenger RNAs (mRNAs) in a sequence-specific manner [ 6 , 7 , 8 ]. (biomedcentral.com)
Electroporation3
  • Unique Virofect technology for efficient transfection of many primary cell types, better than electroporation. (targetingsystems.net)
  • We have overcome this problem by developing a new technology, nanochannel electroporation* (NEP) allowing transfection of many small sized and delicate cells with precise control over dose and timing. (confex.com)
  • Ultimately, the optimized LNP formulation presented ~75% transfection efficiency in umbilical cord blood derived NK cells, with a 5.5-fold increase in fluorescence from eGFP expression compared to the next-best nonviral alternative for NK cell transfection, electroporation. (uu.nl)
Cells32
  • Naked siRNA molecules are generally taken up by the cells at very low efficiencies. (medscape.com)
  • The gene silencing effect was reportedly enhanced when the virus-infected MDCK cells were treated with the immunoliposomes 6-12 h post-infection. (medscape.com)
  • Gene knockdown by transfection of exogenous siRNA is often unsatisfactory because the effect is only transient, especially in rapidly dividing cells. (wikipedia.org)
  • This cell line could be useful for studying embryonic tissue of mammals, such as stem cells and as a transfection host in a variety of cell biology research. (altogen.com)
  • At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. (altogen.com)
  • Transfection efficiency was determined by calculating the ratio of fluorescent to total cells after transfection with a plasmid containing the green fluorescent protein (GFP) gene. (screenfect.com)
  • SDS-PAGE / Western Blot was used to visualise expression of a protein (LRP6 receptor) after its mRNA was targeted in MEF cells upon lrp6 siRNA transfection. (screenfect.com)
  • We demonstrate that co-transfection of the crRNA:tracrRNA duplex into Cas9-expressing cells leads to target gene mutation and loss of target protein expression in the majority of the cell population. (biomedcentral.com)
  • Alternatively, CRISPR components can be delivered to host cells using transfection of a recombinant ribonucleoprotein (RNP) complex consisting of the Cas9 protein with guide RNAs [ 4 ]. (biomedcentral.com)
  • Bivalent siRNA chimera customization enables efficient delivery of bivalent siRNA chimeras and selective targeting of cells to down-regulate the expression of multiple genes. (bocsci.com)
  • 3. Lipoplexes targeting the CD44 hyaluronic acid receptor for efficient transfection of breast cancer cells. (nih.gov)
  • Transfection efficiency of pSEB-siMDR1 DNA was significantly increased by ultrasound microbubble-mediated delivery in rat yolk sac carcinoma L2 (L2-RYC) cells. (biomedcentral.com)
  • MDR1 silencing led to decreased P-glycoprotein activity and drug resistance of L2-RYC cells, which may be explored as a novel approach of combined gene and chemotherapy for yolk sac carcinoma. (biomedcentral.com)
  • Efficient transfection reagents for embryonic and mesenchymal stem cells. (targetingsystems.net)
  • It may also play a key role in the non-viral generation of engineered stem cells and induce pluripotent stem cells with high efficiency and non-carcinogenic properties. (confex.com)
  • The phenotype of "rounded-up" 293 cells were visualized (upper panel) and quantified (lower panel) 48h post transfection with a Nikon microscope. (signagen.com)
  • siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with renilla luciferase gene (0.5 g of pRL-CMV DNA per well) by the above three transfection reagents per manufacturers' protocols into A549 cells growing on a 24-well plate. (signagen.com)
  • Lucifera se-silencing efficiency was calculated relative to untreated cells. (signagen.com)
  • Significant downregulation of β-defensin1 gene ( DEFB1 ) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • In this work we describe the process behind the development of a small interfering RNA (siRNA) delivery system to inhibit ALOX5 in cancer stem cells (CSC), as a model target gene. (cibbim.eu)
  • This combined micelle presented adequate technological properties, safety profile and biological efficacy, resulting in high ALOX5 gene silencing and strong reduction of invasion and transformation capabilities of a stem cell subpopulation isolated from MDA-MB-231 triple negative breast cancer cells. (cibbim.eu)
  • Additionally, silencing of LOC285194 increased the ability of colony formation, cell migration, and invasive capacities, together with blocking the apoptotic rates of GC cells. (puppyreading.com)
  • The induced pacemaker-like cells were analyzed using reverse transcription-quantitative PCR (RT-qPCR) and immunofluorescence to assess the efficiency of differentiation. (spandidos-publications.com)
  • Therefore, the present study assessed a potential new method to improve the efficiency of differentiation of BMSCs into pacemaker-like cells. (spandidos-publications.com)
  • Yet, current methods for gene transfection of NK cells are lacking in efficiency or they induce undesired toxicity or phenotypical changes in the cells. (uu.nl)
  • The reported LNP formulation is an effective non-toxic method for gene delivery in clinically relevant NK cells and may thus provide a suitable platform of phenotypical NK cell optimization for adoptive cancer immunotherapy. (uu.nl)
  • Transcriptome analyses have been extensively used to investigate gene expression in individual cells. (nature.com)
  • c) Compact disc4 silencing … Intravenous administration of siRNA-entrapped LFA-1 I-tsNP silences focus on gene appearance in T cells of humanized mice To. (healthyguide.info)
  • Our findings is the first to reveal that miR-3470b as a novel host factor regulates BEFV replication via directly targeting the MAVS gene in BHK-21 cells and may provide a potential strategy for developing effective antiviral therapy. (biomedcentral.com)
  • Polyplus-transfection has developed a range of products specifically designed for protein production in CHO and HEK-293 cells grown in suspension in synthetic serum-free media. (polyplus-sartorius.com)
  • iNOP-7 was non-toxic, and delivered siRNA with high efficiency to NSCLC cells. (oncotarget.com)
  • In this review, we will briefly discuss the critical issues related to the antitumor efficacy including the structure of CAR, the potential of T cells subsets, the gene modification of lymphocytes, the preconditioning regimens, etc. (biomedcentral.com)
MiRNA1
  • a Most of miRNA genes are transcribed by Pol II and produce greater than 200-nt pre-miRNAs, which contain at least one hairpin structure harboring the miRNA sequence. (biomedcentral.com)
Post-transcriptional level2
  • Alternative splicing is a crucial and ubiquitous mechanism that controls gene expression at the co- and post-transcriptional level. (nature.com)
  • This co-suppression refers to silencing of gene expression principally at a post-transcriptional level. (nih.gov)
ShRNA7
  • The transcription of shRNA gene is driven by a U6 or H1 promoter. (biomedcentral.com)
  • The shRNA targeting the gene of interest (GOI) is placed downstream of the modified U6 promoter with an additional LacO sequence located downstream of the shRNA. (vectorbuilder.kr)
  • Our shRNA stem-loop sequences are optimized to mediate efficient shRNA processing and target gene knockdown. (vectorbuilder.kr)
  • Guaranteed gene silencing with state-of-the-art shRNA designs and multiple promoter and reporter options. (horizondiscovery.com)
  • Because efficient gene silencing depends on both the design and level of expression of the shRNA, it is critical to choose silencing reagents where both the targeting sequence and the specific promoter driving expression are taken into consideration. (horizondiscovery.com)
  • Up to ten shRNA silencing constructs are predesigned for every gene in human, mouse and rat providing broad coverage across the entire genome. (horizondiscovery.com)
  • Recommended for shRNA-induced silencing ( click for data ). (origene.com)
Efficient3
  • Many physical and conjugation methods have also been used for efficient siRNA delivery for the suppression of gene expression. (medscape.com)
  • Establishing an efficient, multiplexed gene knockout system using CRISPR/Cas9 that is both simple and robust in its application would further advance the adoption of CRISPR/Cas9 for genetic studies. (biomedcentral.com)
  • In this study, we present a simple, versatile and highly efficient method to achieve acute gene knockout with CRISPR/Cas9 using chemically synthesized crRNA and tracrRNA oligos. (biomedcentral.com)
Molecules3
  • Candidate molecules are selected by cell-based high throughput screenings and then undergo extensive optimization to generate specific transfection reagents. (screenfect.com)
  • The preparation of siRNA-based chimeric molecules is a good strategy to improve the effect of siRNA-targeted gene silencing. (bocsci.com)
  • SignalSilence ® Atg14 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Atg14 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. (cellsignal.com)
SignaGen Laboratories1
  • 208.00 : SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents. (signagen.com)
Antibody1
  • The Atg14 Antibody confirms silencing of Atg14 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control. (cellsignal.com)
Toxicity4
  • The compound with the highest transfection efficiency and lowest toxicity is Screen F ect ® A. (screenfect.com)
  • Of note, Screen F ect ® A shows an optimal balance of transfection efficiency and cellular toxicity. (screenfect.com)
  • The presence of Pluronic ® F127 in the formulation showed to be of utmost importance, because not only the silencing activity of the polyplexes was improved, but also PEI-associated toxicity was clearly reduced. (cibbim.eu)
  • The ultimate in high efficiency and low toxicity! (origene.com)
Promoter3
  • Promoter methylation represents one of the major epigenetic mechanisms responsible for the regulation of gene expression. (nih.gov)
  • In the absence of tetracycline, the tTS protein derived from the fusion of TetR (Tet repressor protein) and KRAB-AB (the transcriptional repressor domain of Kid-1 protein) binds to the TRE promoter, leading to the active suppression of gene transcription. (vectorbuilder.cn)
  • The rtTA protein, on the other hand, derived from the fusion of a mutant Tet repressor and VP16 (the transcription activator domain of virion protein 16 of herpes simplex virus), binds to the TRE promoter to activate gene transcription only in the presence of tetracycline. (vectorbuilder.cn)
Stably1
Recombinant2
Apoptosis3
MRNA and protein2
  • Additionally, the target association between miR-3470b and mitochondrial antiviral signaling protein (MAVS) was predicted by target gene prediction tools and further validated using a dual-luciferase reporter assay, and the expression of MAVS mRNA and protein levels was negatively associated with miR-3470b levels. (biomedcentral.com)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
Efficacy1
  • Due to the low silencing efficacy obtained, chitosan polyplexes were combined with Pluronic ® -based polymeric micelles with recognized advantages regarding gene transfection. (cibbim.eu)
Delivery4
  • BOC Sciences offers a wide range of siRNA splicing strategies to choose from, and in collaboration with our siRNA delivery system customization services , your gene therapy development research is sure to be enhanced. (bocsci.com)
  • 7. Effect of hyaluronic acid-binding to lipoplexes on intravitreal drug delivery for retinal gene therapy. (nih.gov)
  • During the delivery process, the stable compact structure of the complex, with cationic chitosan chains as the outershell and the anionic genes/drugs as the core, efficiently protects genes/drugs from the ″sweeping effect″ of mucociliary clearance and degradation by enzymes. (sigmaaldrich.com)
  • Chitosan nanoparticles for siRNA delivery: optimizing formulation to increase stability and efficiency. (sigmaaldrich.com)
Experimental2
  • This study may provide experimental evidence for gene therapy in PC. (biomedcentral.com)
  • In addition, we propose to extend this experimental approach to genes associated with other neurodegenerative diseases such as PD. (nih.gov)
Cellular4
  • We show dose control effects on a variety of transfection agents such as oligonucleic acids for cancer therapy, molecular probes for intra-cellular biomarker detection and large plasmid for cell reprogramming. (confex.com)
  • These issues may be overcome by optimizing the cellular phenotype with additional activating receptors or silencing inhibitory receptors, through genetic manipulation. (uu.nl)
  • The silencing of Grp78 expression using short-interference RNA enhanced CdCl 2 -induced cellular damage. (nih.gov)
  • It is important to determine the subcellular compartments that respond to cellular stress induced by cadmium exposure and affect diverse areas of cellular function such as signal transduction, gene expression, cell survival, and death. (nih.gov)
Cell proliferation2
Mutations3
  • Much progress has been gained in identifying mutations and gene products that underlie these disease processes, particularly early onset Alzheimer's disease (AD) and Parkinson's disease (PD). (nih.gov)
  • Despite groundbreaking work in defining the genetic basis for the disease, leading to the description of more than 400 predominantly missense mutations in more than 8 largely sarcomeric genes, therapies remain palliative. (nih.gov)
  • Third, noticed dolutegravir efficiency was not described through ISBT filled with darunavir/ritonavir, lopinavir/ritonavir, or various other boosted PI, also among sufferers contaminated with HIV-1 with principal PI level of resistance mutations. (researchensemble.com)
High4
  • Yolk sac carcinoma have high expression of MDR1 gene [ 21 ], so we hypothesize that small interfering RNA (siRNA) mediated silencing of MDR1 expression would improve the sensitivity of yolk sac carcinoma to chemotherapy drugs. (biomedcentral.com)
  • The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. (biomedcentral.com)
  • Transfection reagents are ready-to-use with a pre-optimized protocol to ensure high transfection efficiency while preserving cell viability and morphology. (polyplus-sartorius.com)
  • Safe harbor TALEN-mediated homologous recombination is a high-efficiency method to generate targeted mini-gene transfer or reporter knock-in cell lines in both human iPSCs and NSCs. (nih.gov)
Significantly2
  • In a Polymerase Chain Reaction (PCR) array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of STAT3. (cdc.gov)
  • Furthermore, the miR-3470b mimic transfection significantly contributed to increase the BEFV N mRNA, G protein level and viral titer, respectively, whereas the miR-3470b inhibitor had the opposite effect on BEFV replication. (biomedcentral.com)
Experiments1
Receptor2
  • According to a TREM2 mechanism, this response is mediated by SYK-NFAT axis and is compromised by blockade and gene silencing of the receptor. (researchsquare.com)
  • The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. (cancerindex.org)
Sequences3
  • It interferes with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, preventing translation. (wikipedia.org)
  • All you need to do is to provide the validated, effective 19 bp sequences from the target genes. (welgen.com)
  • RNA interference is a conserved pathway common to plants and mammals, where double-stranded RNAs (dsRNAs) suppress expression of genes with complementary sequences [ 1-2 ]. (idtdna.com)
DharmaFECT2
  • DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. (horizondiscovery.com)
  • While PepMute and Dharmafect 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, Lipofectamine RNAiMAX gave good knockdown only after 20 nM while enhanced gene expression at low concentration of siRNA (0.5 and 1.0 nM respectively) was observed. (signagen.com)
Interference1
  • R unning interference that is, interfering with the expression of targeted genes has become a popular sport in some scientific circles. (nih.gov)
Cas91
  • CRISPR/Cas9 mediated gene knockout is a powerful tool for genome editing with the ability to target multiple genes simultaneously. (biomedcentral.com)
Target5
  • 90% inhibition in the target gene expression. (medscape.com)
  • For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative. (horizondiscovery.com)
  • These findings indicate that SnoN gene plays an important role in pancreatic cancer development, and might serve as a potential therapeutic target for pancreatic cancer. (biomedcentral.com)
  • Our database ranks all available shRNAs for a target gene in order of their decreasing knockdown scores and recommends testing the top 3 shRNAs with the highest knockdown scores. (vectorbuilder.kr)
  • Customer provides the name, sequence or GeneBank ID of target genes as well as specific cell lines if necessary. (atagenix.com)