It is important to note that measuring the functional titer of a recombinant lentivirus is not only dependent on how efficiently the transfer vector is packaged but also the transduction efficiency of the cell line used. (sigmaaldrich.com)
We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
These multiple possibilities support future use of combinations of compounds to synergistically increase transduction efficiency. (seattlechildrens.org)
In addition to improving gene therapy and delivery of gene-editing tools into cells, increasing transduction efficiency using small molecule agents could facilitate using of hemopoietic stem cells for protective functions. (seattlechildrens.org)
On the other hand, Ad vectors also have a number of disadvantages, including their low transduction efficiency in cells lacking coxsackievirus-adenovirus receptor (CAR) and their induction of innate immune responses. (hindawi.com)
Improved transduction efficiency may allow intravitreal injection to become the preferred route for delivering gene therapy to both the inner and outer retina. (molvis.org)
Intravitreal injection has been used to transduce retinal ganglion cells and bipolar cells, but at present this approach produces relatively low efficiency retinal transduction. (molvis.org)
In the present study the cellular tropism and transduction efficiency of seven AAV vector serotypes (AAV1, 2, 3, 4, 5, 6, 8) and an integration-deficient lentiviral vector were assessed for their ability to transduce corticospinal neurons (CSNs) following intracortical injection. (nih.gov)
Currently, quantum transduction demonstrations are limited in conversion efficiency, with most schemes operating in the high-pump regime, therefore with large noise. (fnal.gov)
The high microwave quality factor and large coupling strength between microwave and optical modes are expected to lead to orders of magnitude enhancements for transduction efficiency at a low pump power of tens of μW. (fnal.gov)
Transduction Efficiency of Zika Virus E Protein Pseudotyped HIV-1 gfp and Its Oncolytic Activity Tested in Primary Glioblastoma Cell Cultures. (bvsalud.org)
Using five different GBM primary cell cultures and three laboratory -adapted GBM cell lines , we showed that ZIKV /HIVgfp achieved a 4-6 times higher transduction efficiency compared to the commonly used VSV/HIVgfp. (bvsalud.org)
Signal transduction mechanisms2
There are several signal transduction mechanisms that modulate intracellular calcium concentration and therefore the state of vascular tone. (cvphysiology.com)
Although HPV was described decades ago, the underlying oxygen sensing and signal transduction mechanisms remain obscure. (ersjournals.com)
Lentiviral7
Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. (sigmaaldrich.com)
Keep them stored on ice while performing lentiviral transductions. (sigmaaldrich.com)
A common method of transferring new genes or gene-editing components into cells, to provide novel functions, is by transduction of engineered lentiviral or other vectors. (seattlechildrens.org)
Following a single in vivo lentiviral transduction, genetically tagged AMs persisted in lung alveoli and expressed transferred genes for the lifetime of the adult mouse. (jci.org)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Intracellular1
This subclass comprises research and experimental development in intracellular signaling, signal transduction, receptor and membrane biology. (ubc.ca)
Mechanisms3
Therefore, the understanding of the diverse signalling molecules, signal recognition mechanisms and signal transduction pathways is fundamental for the insight into physiological and pathophysiological processes. (mdpi.com)
The focus of this article is applied physiology of the inner ear, emphasizing the processes involved in transduction and the homeostatic mechanisms necessary for maintaining the inner ear in a functional state. (medscape.com)
oncogene activation, mechanisms of signal transduction, and genetic linkage analysis. (cdc.gov)
Sensory transduction1
While performing this sensory transduction process, the inner ear analyzes a sound stimulus in terms of its frequency, intensity, and temporal properties, and it transmits this information to the CNS for further processing and interpretation. (medscape.com)
Specificity3
Limitations in the usage of AAV in murine models include cell types that are refractory to transduction and difficulties achieving tissue-specificity. (nature.com)
Here we explain some specifications of signal transduction : a) Specificity : Any signal molecules fit into the binding site with its complementary receptor and other signals never fit. (assignmenthelp.net)
Tests of GBM tumor specificity by transduction of GBM tumor and normal brain cells showed a high specificity for GBM cells . (bvsalud.org)
Receptors2
Diverse signal recognition receptors and signal transduction pathways were defined which regulate cellular functions, metabolism and development. (mdpi.com)
We have examined NGF-induced signal transduction events and neuronal differentiation in MAH cells, a neuronal progenitor cell line, in which the expression of the two NGF receptors, p140^(trk) (Trk) and p75^(LNGFR) (p75), has been independently manipulated. (caltech.edu)
Vectors2
Here we describe a model system, SELective Expression and Controlled Transduction In Vivo (SELECTIV), that enables efficient and specific expression of transgenes by coupling adeno-associated virus (AAV) vectors with Cre-inducible overexpression of the multi-serotype AAV receptor, AAVR. (nature.com)
however, the transduction properties of Ad vectors in neonates and infants have not been fully examined. (hindawi.com)
Modulate1
These data provide evidence that p75 can modulate signal transduction through Trk in a neuronal progenitor cell context and that such modulation has functional consequences for the neuronal differentiation pathway induced by NGF. (caltech.edu)
Transmembrane1
We review current evidence for the presence and activity in plants of several paradigmatic components of transmembrane signal transduction systems. (rti.org)
Progenitor cell1
iii) Neonates and infants have higher stem/progenitor cell ratios, and thus transduction into stem/progenitor cells may occur more efficiently than in adults. (hindawi.com)
Cells9
The Innate Immunity Signal Transduction in Human Leukocytes is a research study to determine the response of immune cells from the bloodstream. (nih.gov)
By directing AAV transduction through the regulated expression of its receptor, the approach provides precise control over which cells express the AAV-vectored transgene. (nature.com)
For example, Dr. Torbett's group is studying polyphenols as transduction enhancers based on their results showing that the resveratrol-like compound caraphenol A dose-dependently increases gene delivery and genomic integration into human cells. (seattlechildrens.org)
The small compounds boost transduction into a variety of cells including other types of stem cells and T cells for CAR T therapy. (seattlechildrens.org)
The oxygen sensing and signal transduction machinery is located in the pulmonary arterial smooth muscle cells (PASMCs) of the pre-capillary vessels, albeit the physiological response may be modulated in vivo by the endothelium. (ersjournals.com)
Without the addition of enzymes, transduction was limited to occasional cells in the retinal ganglion cell layer. (molvis.org)
British Library EThOS: Lipopolysaccharide-activated signal transduction in cardiac and vascular smooth cells. (bl.uk)
Although there is much debate over the mechanism by which this "protein transduction" occurs, the ability of CPPs to translocate rapidly into cells is being exploited to deliver a broad range of therapeutics including proteins, DNA, antibodies, oligonucleotides, imaging agents and liposomes in a variety of situations and biological systems. (benthamscience.com)
The ↑[Ca 2+ ] i activates a signaling cascade that involves, sequentially, calcium-calmodulin, nitric oxide synthase, guanylyl cyclase, and protein kinase G. This signal transduction pathway enables Cl − to pass through vesicular channels and act as a counterion for the transport of H + , so that the vesicles of secretogogue-stimulated cells become acidic. (jneurosci.org)
Infectious Disease3
Prenatal exposure to arsenic and cadmium impacts infectious disease-related genes within the glucocorticoid receptor signal transduction pathway. (cdc.gov)
This research aimed to identify key genes/signal transduction pathways that associate prenatal exposure to these toxic metals with changes in infectious disease susceptibility using a Comparative Genomic Enrichment Method (CGEM). (cdc.gov)
Given their common identification across numerous human cohorts and their known toxicological role in disease, the identified genes within the glucocorticoid signal transduction pathway may underlie altered infectious disease susceptibility associated with prenatal exposures to the toxic metals iAs and Cd in humans. (cdc.gov)
Genetic1
Genetic transduction is a major evolutionary force that underlies bacterial adaptation. (gla.ac.uk)
Mice2
Accordingly, this study examined the properties of Ad vector-mediated transduction in neonatal mice. (hindawi.com)
In this study, we tried to elucidate signal transduction pathway in primitive hematopoietic progenitors of mice stimulated with interleukin-11(IL-11). (nii.ac.jp)
Pathway3
Two signal transduction pathways are linked to Gq-proteins: phospholipase C pathway (forms inositol triphosphate, IP 3 ) and the Rho-kinase pathway. (cvphysiology.com)
This dissertation focuses on understanding some of the biochemical events that take place in the chemotaxis signal transduction pathway of bacteria. (umd.edu)
A set of 82 infection and exposure-related genes was identified and found to be enriched for their role in the glucocorticoid receptor signal transduction pathway. (cdc.gov)
Pathways6
Pancreatic beta-cell signal transduction is complex and involves a well-regulated interaction of a number of signals generated by the metabolism of glucose and the activation of a variety of receptor-operated pathways. (ki.se)
Both bacteria and archaea use chemosensory arrays and downstream signal transduction pathways to receive and process external stimuli. (esrf.fr)
Signal transduction pathways in hepatocyte cell death. (rug.nl)
The investigation of signal transduction pathways is critical to the basic understanding of cellular processes as these pathways function to regulate diverse processes in both eukaryotes and prokaryotes. (umd.edu)
Based on these findings, we developed a computational synthetic biology framework for design and re-engineering signal transduction networks and pathways that involve cross-talk between molecular chaperones and protein kinase clients. (utoronto.ca)
CaR stimulation leads to the activation of multiple signal transduction pathways. (jneurosci.org)
Protein2
Protein Transduction: Cell Penetrating Peptides and Their Therapeutic Applications, Frontiers in Medicinal Chemistry (2010) 5: 98. (benthamscience.com)
The allosteric interactions and regulation of molecular chaperones and protein kinases allow for molecular communication and event coupling in signal transduction networks. (utoronto.ca)
Biochemistry1
Combining X-ray crystallography data from beamline ID30B and biochemistry with cell biology reveals how the conserved signal transduction machinery in bacteria and archaea is coupled to the archaeal rotary motor termed the archaellum. (esrf.fr)
Improves2
Experiment results demonstrate that the proposed method improves the traditional graph transduction by as much as 15% in accuracy and AUC in all common situations of distribution difference. (videolectures.net)
To determine whether the co-injection of extracellular matrix degrading enzymes improves retinal transduction following intravitreal delivery of adeno-associated virus-2 (AAV2). (molvis.org)
Mechanism1
Network-defined modularity of interacting components and cross-talk in signal transduction networks are quantified through molecular mechanism of allosteric regulation. (utoronto.ca)
Research2
Professor Per-Olof Berggren is research group leader of the group Signal Transduction at the Department of Molecular Medicine and Surgery. (ki.se)
We present ongoing and future research on optical-microwave transduction, which would have an impact on single-photon sensing and networks, with the ability to perform measurements below the standard quantum limit (SQL). (fnal.gov)
Molecules1
The Torbett group is screening for and identifying other small molecules such as prostaglandin E2 to add to their toolbox of small molecules that enhance transduction. (seattlechildrens.org)
Greatly1
The addition of heparinase III or chondroitin ABC lyase greatly enhanced transduction of the retinal ganglion cell layer and increased the depth of transduction into the outer retina. (molvis.org)
Generate1
Our findings indicate that C. difficile employs phase variation of the CmrRST signal transduction system to generate phenotypic heterogeneity during infection, with concomitant effects on bacterial physiology and pathogenesis. (nih.gov)
Plant1
At the current stage of development of the field of plant signal transduction, broad similarities between plant and the more well-studied animal systems are apparent. (rti.org)
Cell2
We focus on Signal transduction in the pancreatic beta-cell. (ki.se)
Here we focus on the flagellated bacterium {\it E. coli}, for which a great deal is known about signal detection, transduction and cell-level swimming behavior. (mpg.de)
Approach3
Our approach exploits the absolute dependence of AAV transduction on the expression of its receptor 22 , AAVR (also named KIAA0319L). (nature.com)
Furthermore, the intravitreal approach can potentially deliver more widespread transduction across the retina when compared to localized subretinal blebs. (molvis.org)
In particular, we generalize the moment-based approach to macroscopic equations used earlier to higher dimensions and show how aspects of the signal transduction and response enter into the macroscopic equations. (mpg.de)
Higher1
Electroretinograms survived with higher concentrations of heparinase III and chondroitin ABC lyase than were required for optimal retinal transduction. (molvis.org)