• The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. (hmdb.ca)
  • Effects of EGCG on nuclear factor kappa B (NFκB) and activator protein-1 (AP-1) transcriptional activity were assessed by reporter gene assay. (molvis.org)
  • In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. (qedbio.com)
  • The disease is characterized by an oxidant / antioxidant imbalance in the lungs leading to activation of redox-sensitive transcription factors e.g. activator protein 1 (AP-1) and nuclear factor kappa B (NF-kappa B). We have previously described PNRI-299, a small molecule beta-strand mimetic template compound, as an inhibitor of the multifunctional AP endonuclease / redox factor 1 (Ref-1) [1]. (eurekaselect.com)
  • Currently, the clinical interventions for patients with BC-bone metastasis (BC-BM) are bisphosphonates (BPs) or denosumab (an anti-receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody), which disrupt the vicious cycle by targeting osteoclasts [ 6 ]. (biomedcentral.com)
  • We have identified and characterized in infected cells the transcriptional activator, NF-κ B, whose recognition motifs have been identified in several gene enhancers important for lymphocyte-specific gene expression. (columbia.edu)
  • The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. (cusabio.com)
  • In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. (cusabio.com)
  • Direct measurement of NF-kappaB-regulated genes revealed that their extent of activation was greater in the apoE4 mice. (nih.gov)
  • The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. (hmdb.ca)
  • p50 binds to the kappa-B consensus sequence 5'- GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. (hmdb.ca)
  • O NF- k B é um fator de transcrição envolvido no controle da expressão de diversos genes ligados à resposta inflamatória. (usp.br)
  • O NF- k B e os IFNGR1 e INFGR2 são necessários para a expressão dos genes NCF1 e NCF2 e para a ativação do sistema NADPH oxidase humano neste sistema modelo. (usp.br)
  • The NF- k B is a transcriptional factor involved in the expression of several genes related to the inflammatory response. (usp.br)
  • We studied the NF- k B and the IL-12/23-IFN- g axis defects consequences on the regulation of CYBA, NCF1, NCF2 and NCF4 genes of the human NADPH oxidase system in U937 cells, and in B EBV cells from patients with EDA-ID, DGC, or patients with IL-12/23-IFN- g axis defects. (usp.br)
  • Biological Function Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. (qedbio.com)
  • In macrophages, TonEBP is a transcriptional cofactor of NF-κB which stimulates the transcription of pro-inflammatory genes in response to LPS. (biomedcentral.com)
  • In macrophages, TonEBP regulates transcription of pro-inflammatory genes such as tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) as a transcription cofactor of NF-κB independent of DNA binding [ 11 ]. (biomedcentral.com)
  • Inhibition of MAP kinase/NF-kB mediated signaling and attenuation of lipopolysaccharide induced severe sepsis by cerium oxide nanoparticle s. (cdc.gov)
  • This stabilization and retention of IκB in the nucleus resulted in inhibition of NFκB and transcriptional suppression of the critical antiapoptotic protein, survivin. (aacrjournals.org)
  • Pre-treatment of cells with the novel Gq/11 inhibitor YM-254890 and Gq/11 siRNA caused a similar pattern of inhibition and also reduced PAR-2-mediated NFκB transcriptional activity. (strath.ac.uk)
  • Inhibition of Gi or G12/13 pathways by pertussis toxin pre-treatment or over-expression of the RGS mutant Lsc, also did not effect NFκB phosphorylation. (strath.ac.uk)
  • Additionally, inhibition of NF-κB activity in HCC cells abrogated URG4/URGCP-induced NF-κB activation and angiogenic capacity. (biomedcentral.com)
  • Has anti-apoptotic effects due to inhibition of the transcriptional activity of p53. (antibodies-online.cn)
  • disables inhibition of NF-kappa-B DNA-binding activity. (lu.se)
  • Immunohistochemistry experiments revealed that microglial and NF-kappaB activation were more pronounced in apoE4 than in apoE3 mice. (nih.gov)
  • These analyses suggest that an integrated NF-κB system network underlies activation of both RelA and RelB containing dimer and that a malfunctioning canonical pathway will lead to an aberrant cellular response also through the non-canonical pathway. (wikipedia.org)
  • In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. (hmdb.ca)
  • The role of nuclear factor kappa B (NF- k B) and the IL-12/23-IFN- g axis in the activation of the NADPH oxidase system. (usp.br)
  • The NF- k B and the IFNGR1 and INFGR2 are necessary for NCF1 and NCF2 gene expression and activation of the human NADPH oxidase in this model system. (usp.br)
  • We previously showed that genotoxic stress induced an active extracellular release of nucleophosmin (NPM) in human cardiac mesenchymal progenitor cells, and that serum deprivation provokes NPM secretion from human endothelial cells, eliciting inflammation via nuclear factor kappa B (NF-kB) transcriptional activation. (frontiersin.org)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • Parallel experiments showed that in both cell types, p65 NFκB phosphorylation is mediated through the selective activation of IKK2. (strath.ac.uk)
  • The mechanism underlying circIKBKB-mediated activation of NF-κB/bone remodeling factors signaling and EIF4A3-induced circIKBKB were investigated using RNA pull-down, luciferase reporter, chromatin isolation by RNA purification and enzyme-linked immunosorbent assays. (biomedcentral.com)
  • Building on these observations, we found a specific activation of NF-κB and a block of IRF3 nuclear translocation in SARS-CoV-2 infected cells. (nature.com)
  • This NF-κB response was mediated by cGAS-STING activation and could be attenuated through several STING-targeting drugs. (nature.com)
  • One unknown link in the activation of NF-κB was the ubiquitin ligase that catalyzes generation of the active p50 subunit from its p105 inactive precursor. (iucc.ac.il)
  • Cerulenin disrupted the assembly of the TonEBP/NF-κB/AP-1/p300 complex and suppressed the LPS-induced microglial activation and the neuronal damages in animals. (biomedcentral.com)
  • Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. (biossusa.com)
  • Promotes proteolytic processing of NFKB2/P1, which leads to activation of NF-kappa-B via the non-canonical pathway. (biossusa.com)
  • In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. (cusabio.com)
  • In particular, NPM is directly associated with the DNA-binding domain of NF-kB p65 subunit, enhancing its DNA-binding activity. (frontiersin.org)
  • Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. (cusabio.com)
  • Kupffer cells and macrophage cells exposed to CeO2NPs exhibited decreases in LPS-induced cytokine release (TNF-alpha, IL-1beta, IL-6, HMGB1) which were associated with diminished cellular ROS, reduced levels of nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), and decreased nuclear factor-kappa light chain enhancer of activated B cells (NF-kB) transcriptional activity. (cdc.gov)
  • A luciferase reporter assay was used to examine the transcriptional activity of nuclear factor kappa - light - chain - enhancer of activated B cells (NF-κB). (biomedcentral.com)
  • EGCG significantly inhibited phosphorylation of the MAPKs p38 and c-Jun N-terminal kinase (JNK), and NFκB and AP-1 transcriptional activities. (molvis.org)
  • Furthermore, overexpressing URG4/URGCP increased - whereas knockdown of URG4/URGCP decreased - VEGFC expression, NF-κB transcriptional activity, the levels of phosphorylated (but not total) IκB kinase (IKK) and IκB-α, and expression of TNFα, IL-6, IL-8 and MYC in HCC cells. (biomedcentral.com)
  • NF- kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. (hmdb.ca)
  • maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. (rcsb.org)
  • NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. (cusabio.com)
  • These findings suggest that the increased brain inflammation in apoE4 mice is related to disregulation of NF-kappaB signaling pathway. (nih.gov)
  • NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. (hmdb.ca)
  • NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. (cusabio.com)
  • His work investigates the relationship between Cancer research and topics such as Inflammation that intersect with problems in Downregulation and upregulation, Transcriptional regulation, Clinical significance and Intracellular. (research.com)
  • Further mechanism study disclosed that CypA could regulate the transcriptional activity of NF-κB, the pivotal transcriptional factor regulating M1 polarization, dependent of its PPIase activity. (nih.gov)
  • this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (By similarity). (rcsb.org)
  • and exerts a dominant negative effect on the transcriptional activity of hGRα. (medscape.com)
  • It also regulates the stability and activity of transcription factors including p53 and nuclear factor kappa B (NF-κB) ( 5 , 6 ). (frontiersin.org)
  • Pretreatment with the proteasome inhibitor carfilzomib can further stabilize IκB, which is sequestered in the nucleus to inhibit transcriptional activity of NFκB and suppresses expression of survivin, thereby inducing apoptosis. (aacrjournals.org)
  • Taken together these data indicate dependency for Gq/11 in early phosphorylation of p65 NFκB and this subsequently affects initial NFκB-dependent gene transcriptional activity, however later regulation of p65 is unaffected. (strath.ac.uk)
  • TonEBP deficiency blocked the LPS-induced expression of pro-inflammatory cytokines and enzymes in association with decreased activity of NF-κB in BV2 cells. (biomedcentral.com)
  • We found that there was also a decreased activity of AP-1 and that TonEBP was a transcriptional cofactor of AP-1 as well as NF-κB. (biomedcentral.com)
  • Overall these novel data demonstrate an IKK2-dependent, predominantly G-protein-independent pathway involved in PAR-2 regulation of NFκB phosphorylation in keratinocytes. (strath.ac.uk)
  • May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. (lu.se)
  • In this pathway, NIK stimulates the processing of p100 into p52, which in association with RelB appears in the nucleus as RelB:p52 NF-κB heterodimers. (wikipedia.org)
  • Free NF-κB then enters the nucleus to initiate its transcriptional program. (iucc.ac.il)
  • Tax is a transcriptional co-factor that interfere several signaling pathways related to anti-apoptosis or cell proliferation. (kegg.jp)
  • The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. (cusabio.com)
  • NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. (rcsb.org)
  • The mechanisms underpinning the coupling of GPCRs, such as PAR-2, to the phosphorylation of p65 NFκB have not been investigated. (strath.ac.uk)
  • Mechanically, circIKBKB activated NF-κB pathway via promoting IKKβ-mediated IκBα phosphorylation, inhibiting IκBα feedback loop and facilitating NF-κB to the promoters of multiple bone remodeling factors. (biomedcentral.com)
  • Tyrosine-phosphorylation could only lead to dissociation from NF-kappa-B. (lu.se)
  • We previously showed that physiologic breaks initiate lymphocyte development-specific transcriptional programs. (biomedcentral.com)
  • We identified a central lymphocyte-specific transcriptional response common to both physiologic and genotoxic breaks, which includes many lymphocyte developmental processes. (biomedcentral.com)
  • While it is known that genotoxic agents, such as IR, activate transcriptional programs involved in maintaining the integrity of the genome, we also want to investigate whether or not the genotoxic breaks could affect lymphocyte-specific maturation transcriptional responses similar to those we observed following RAG-induced physiological DSBs. (biomedcentral.com)
  • The inactive cytoplasmic precursor could be activated by treatment of extracts with deoxycholate, which was shown previously to dissociate NF-κ B from an inhibitor, I kappa B. Treatment of lymphocyte extracts with 3 mM GTP stimulated NF-κ B binding to its recognition motif in vitro, thereby distinguishing it from a related nuclear factor, H2-TF1. (columbia.edu)
  • NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. (hmdb.ca)
  • NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF- kappa-B inhibitor (I-kappa-B) family. (hmdb.ca)
  • Subsequently, the inhibitory proteins that sequester NF-κB in the cytoplasm are phosphorylated, ubiquitinated, and degraded in the proteasome. (iucc.ac.il)
  • Our findings provide evidence that PPIase CypA promoted macrophages polarization toward pro-inflammatory M1 phenotype via transcriptional activating NF-κB, thus leading to aggravated arthritis. (nih.gov)
  • Overexpression of KPC1 probably shifts the balance between NF-κB subunits from the "canonical" p50·p65 heterodimer to a p50 homodimer which results in a strong tumor suppressive phenotype. (iucc.ac.il)
  • NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. (hmdb.ca)
  • Guido Franzoso mostly deals with Transcription factor, Cell biology, NF-κB, Tumor necrosis factor alpha and NFKB1. (research.com)
  • His primary areas of investigation include Cancer research, Cancer, NF-κB, NFKB1 and Pathogenesis. (research.com)
  • Interestingly, NPM interacts with Toll-like receptor (TLR)4 in these cells and activates an NF-kB-dependent inflammatory pathway upregulating interleukin IL-6 and COX-2 gene expression. (frontiersin.org)
  • Theileria-infected lymphocytes contained constitutively high levels of activated NF-kappa B in nuclear fractions and inactive NF-κ B in cytoplasmic fractions. (columbia.edu)
  • Selective killing of the parasite, which left the host cells intact, resulted in a rapid loss of NF-κ B from the nuclear fractions and a slower loss from the cytoplasmic fractions. (columbia.edu)
  • Infection with the Intracellular Protozoan Parasite Theileria parva Induces Constitutively High Levels of NF-κ B in Bovine T Lymphocytes. (columbia.edu)
  • Comparing these transcriptional responses provides a greater understanding of the mechanisms cells use in the differentiation between types of DNA damage and the potential consequences of different sources of damage. (biomedcentral.com)
  • Transcriptional Control of Regulatory T Cells in Cancer: Toward Therapeutic Targeting? (mdpi.com)
  • Our results show that SARS-CoV-2 directs a cGAS-STING mediated, NF-κB-driven inflammatory immune response in human epithelial cells that likely contributes to inflammatory responses seen in patients and could be therapeutically targeted to suppress severe disease symptoms. (nature.com)
  • NF-κ B is normally constitutively activated in nuclear extracts derived from B cells and can be induced in T cells and nonlymphoid cells by phorbol esters. (columbia.edu)
  • In parasitized cells, NF-κ B could not be further stimulated by treatment with 12-O-tetradecanoylphorbol-13-acetate whereas in cells treated to remove the parasite, this compound stimulated elevated levels of NF-κ B. We propose that high levels of activated NF-κ B are maintained by the presence of the parasite in infected T cells. (columbia.edu)
  • Guido Franzoso combines subjects such as Transcription factor, Pathogenesis and Multiple myeloma with his study of NF-κB. (research.com)
  • An important signaling "code" in the multiple-step NF-κB activating cascade consists of different ubiquitin (Ub) chains that are assembled on the different effector proteins through different lysine residues in the Ub molecule. (iucc.ac.il)
  • The nuclear factor-kappa B (NF-κB) transcription factor plays an essential role in the host immune response to different pathogens and genotoxic stimuli. (iucc.ac.il)
  • A variety of biological functions carried out by NF-κB, depends on the dimeric composition of the transcription factor, and can determine the tumor suppression/promotion fate of the cell. (iucc.ac.il)
  • The scientist's investigation covers issues in Cell biology, Transcription factor, NF-κB, Programmed cell death and Cancer research. (research.com)
  • Here we compare transcriptional responses to physiological DNA breaks with responses to genotoxic DNA damage induced by ionizing radiation. (biomedcentral.com)
  • Thus, this novel mechanism by which selinexor suppresses NFκB signaling provides unique opportunities for cancer therapy, especially when this drug is combined with a proteasome inhibitor. (aacrjournals.org)
  • We revealed a plausible mechanism for circIKBKB-mediated NF-κB hyperactivation in bone-metastatic BC, which might represent a potential strategy to treat breast cancer bone metastasis. (biomedcentral.com)
  • Guido Franzoso mainly focuses on Cancer research, Cancer, NF-κB, Cancer cell and Carcinogenesis. (research.com)
  • A select set of TNF-R superfamily members, including lymphotoxin β-receptor (LTβR), BAFF-R, CD40 and RANK, activate the non-canonical NF-κB pathway. (wikipedia.org)
  • Recent studies has suggested that the functional non-canonical NF-κB pathway is modulated by canonical NF-κB signalling. (wikipedia.org)
  • These genome wide transcriptional responses are very tightly regulated and complex. (biomedcentral.com)
  • By comparing the transcriptional responses to both types of DNA damage, we can compare the similarities in the responses to damage as well as the differences induced by genotoxic damage. (biomedcentral.com)
  • NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. (hmdb.ca)
  • The subcellular distributions of IκB and NFκB are indicative of carcinogenesis. (aacrjournals.org)
  • Cerulenin was used to block the NF-κB cofactor function of TonEBP. (biomedcentral.com)
  • Here, we have evaluated transcriptional and cytokine secretion profiles and detected a distinct upregulation of inflammatory cytokines in infected cell cultures and samples taken from infected patients. (nature.com)
  • His research integrates issues of Carcinogenesis and Programmed cell death in his study of NF-κB. (research.com)