Transcription factors protein expressed sequence tags. Medical search. Frequent questions

We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
A ) Pools of homology repair template plasmids containing different protein tags were co-injected with a plasmid encoding an sgRNA targeting the N-terminus of the target coding sequence into embryos expressing transgenic Cas9. (elifesciences.org)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

Of these, 29 were found to correspond to known genes, 22 matched expressed sequence tags in public databases and 21 showed no sequence similarity to any existing entries. (elsevierpure.com)
Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
describe a novel method to identify potential transcription factors from a list of differentially expressed genes using textual similarity between the genes and transcription factors reported in the literature [ 30 ]. (biomedcentral.com)
Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)

6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
After Blast analysis within GenBank, 64% could be identified, 21% were homologous to unannotated expressed sequence tag (EST) or genomic sequences, and 15% were novel. (appliedbioinfo.com)
Sample-specific meta-genomic assembled genomes (MAGs) were used as reference genomes to accurately identify the origin of RNA reads, and transcript ratios of genes with opposite transcription responses were compared to eliminate biases related to differences in organismal abundance, an approach hereafter named the "diametric ratio" method. (osti.gov)

You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)

The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). (geneticsmr.com)
After randomly choosing, cloning and sequencing, 227 ESTs with high quality were obtained and submitted to GenBank database. (geneticsmr.com)
Using BLASTX, 79.7% of the ESTs shared homology with known functional DNA sequences and 20.3% shared significant homology with unknown proteins. (geneticsmr.com)
Functional genomics has its foundation in the large-scale projects for collecting expressed sequence tags (ESTs) and for genome sequencing, begun in the 1980s. (biomedcentral.com)
Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)

Two key mechanisms in this context are methylation and acetylation, both of which involve modifications to the DNA molecule or to the histone proteins around which DNA is wound. (prohealth.com)
On the other hand, histone acetylation involves the addition of an acetyl group (CH3CO) to the histone proteins around which DNA is wound. (prohealth.com)
The DNA is wrapped around these histone proteins to form a structure called chromatin, which can be loosely or tightly packed. (prohealth.com)
The protein structure that surrounds the DNA, chromatin, largely comprises histone proteins and can be open or closed and thus allow or prevent factors binding to the DNA. (lu.se)
Epigenetic modifications of the histone proteins, such as acetylation or methylation, affect how tightly the DNA is packed around the histones and thus how accessible the DNA sequence is. (lu.se)
As well as DNA methylation, there are also other epigenetic mechanisms such as modification of histone proteins. (lu.se)

To define the molecular basis of the IL-6-induced mitogenic response in myeloma cells, we applied STAR (subtractive transcriptional amplification of mRNA), a new differential expression analysis technology, to isolate mRNAs preferentially expressed in IL-6-treated versus untreated cultures of the factor-responsive myeloma cell line U266. (elsevierpure.com)
Desiccation tolerance of peanut seeds was achieved during 45 to 65 DAP (days after pegging) embryogenesis, while a set of low molecular weight (9 to 15.5 kD) heat-stable polypeptides was preferentially expressed. (jipb.net)
Genes preferentially expressed in bovine oocytes revealed by subtractive and suppressive hybridization. (appliedbioinfo.com)
B-cell translocation gene 4, cullin 1, MCF.2 transforming sequence, a locus similar to snail soma ferritin, and three unidentified genes were, indeed, preferentially expressed in the oocyte, even though most were also highly expressed in testis. (appliedbioinfo.com)

The purity of the protein is greater than 90% assayed by SDS-PAGE. (cusabio.com)

Mercator is a tool to batch classify protein or gene sequences into MapMan functional plant categories. (gabipd.org)
The study seeks to identify putative regulatory motifs in the upstream regions of 13 functional groups of genes expressed in the intraerythrocytic developmental cycle of Pf . (biomedcentral.com)
The 'CACA motif' was absent in functional groups expressed during the ring to early trophozoite transition. (biomedcentral.com)
For functional groups expressed in each transition, the motifs tended to be similar. (biomedcentral.com)
In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
When expressing membrane proteins, researchers must assume from the start that the protein will be difficult to express in functional form. (neb.com)
As a solution NEB offers a tunable T7 expression strain for the production of membrane proteins and other difficult targets so that the level of functional protein may be optimized. (neb.com)

Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Based on oil accumulation dynamics of V. galamensis seeds, we harvested seed samples from three stages (17, 38, and 45 days after pollination, DAP) representing the initial, fast and final EFA accumulation phases, and one mixed sample from different tissues for RNA-sequencing, with three biological replicates at each sample. (researchsquare.com)
HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
We investigated the expression profile of several candidates in the oocyte and a panel of gonadal and somatic tissues by reverse transcription-polymerase chain reaction. (appliedbioinfo.com)

The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. (wikipedia.org)
This family has structurally related proteins that appear to have antiproliferative properties. (appliedbioinfo.com)

We hypothesized that the apple homolog(s) of AINTEGUMENTA ( ANT ), an APETALA2-repeat containing transcription factor, regulates cell production during fruit growth in apple. (biomedcentral.com)

However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)

and they are associated with altered histone acetylation and transcription factor (nerve growth factor-induced clone A [NGFIA]) binding to the glucocorticoid receptor promoter. (deepdyve.com)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
This clone was engineered to express the complete ORF with an expression tag. (origene.com)
The subtracted library was characterized by sequencing 185 random clone inserts, representing 146 nonredundant genes. (appliedbioinfo.com)

Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
NEB also offers solutions for expressing these types of proteins, either in vitro or in vivo. (neb.com)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

This means that while our DNA sequence remains the same, how it's read and expressed can change based on these epigenetic modifications, significantly impacting our health and development. (prohealth.com)
EGFRIII changes the cell's internal signaling network, its uptake and use of nutrients, key elements of its gene-reading machinery known as transcription factors and its epigenetic landscape--the distribution of chemical tags that determines which parts of its genome are available for reading. (sciencedaily.com)
Using technology developed in Ren's laboratory, the researchers began by profiling EGFRvIII's specific epigenetic activation of DNA sequences known as "enhancers. (sciencedaily.com)
Epigenetic changes also take place throughout our lives as a result of normal ageing, lifestyle and environmental factors. (lu.se)
Epigenetic changes mainly take place through chemical modification of DNA (DNA methylation) and histones, the proteins around which the DNA helix winds itself. (lu.se)

This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
Some viruses hijack cellular machinery to translate some of their mRNAs through a noncanonical initiation pathway using internal ribosome entry site (IRES), a highly structured RNAs which can directly recruit the ribosome with a restricted set of initiation factors, and in some cases even without cap and initiator tRNA. (cnrs.fr)
however, mRNAs encoding a subset of factors important for error-free replication and chromosome segregation, including TIPIN, PLK2 and PLK3, remain at low abundance. (babraham.ac.uk)

Epigenetics, the study of changes in gene expression not caused by alterations in the DNA sequence, has revolutionized our understanding of genetics and its impact on health and longevity. (prohealth.com)
It does this by physically impeding the binding of transcription factors and other machinery necessary for gene expression. (prohealth.com)
This results in a more open or "relaxed" chromatin structure, enabling greater access for the transcription machinery, thereby promoting gene expression. (prohealth.com)
Among the known genes uncovered in the screen were those encoding proteins that function in cell division, cell signalling and gene/protein expression. (elsevierpure.com)
The acquisition and induction of desiccation tolerance associated with the expression of heat-stable proteins in the developing peanut (Arachis hypogaea L. ) seeds were studied. (jipb.net)
CUSABIO transfected the expression vector which inserted the recombinant DNA into the E.coli, cultured the cells, and then induced the transcription and translation of the cloned vector. (cusabio.com)
TFBMs act as binding sites for transcription factors and coordinate the expression of the genes in whose promoter regions they appear. (biomedcentral.com)
Expression profiling is the direct offspring of these sequencing projects. (biomedcentral.com)
With genome or EST sequences in hand, microarrays or macroarrays can be constructed to analyze gene-expression profiles at the genome-wide level over a range of conditions. (biomedcentral.com)
Like Zap1p, these factors activate the expression of target genes in metal-limiting conditions, to facilitate uptake and distribution of the metal ion. (biomedcentral.com)
As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. (betalifesci.com)
In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
Most of the enhancers activated by EGFRvIII bore signature DNA sequences that are bound by dozens of transcription factors--regulators of gene expression--expressed at high levels in GBM. (sciencedaily.com)
This protein controls the expression and activity of yet another transcription factor, cMyc, which plays a central role in driving the aberrant metabolism and growth of GBM cells. (sciencedaily.com)
HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
The factors responsible for the postnatal and starvation-associated induction of HMGCS2 gene expression have been identified and shown to involve changes in circulating hormone and fatty acid levels. (aacrjournals.org)
In addition, this indel mutation significantly increased StAR mRNA expression by disrupting the ability of the FOXL2 protein to bind to the StAR promoter and act as a repressor of this gene. (ijbs.com)
Laser microdissection unravels cell-type-specific transcription in arbuscular mycorrhizal roots, including CAAT-box transcription factor gene expression correlating with fungal contact and spread. (uni-bielefeld.de)
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express. (neb.com)
Thanks for holding, and welcome to the New England BioLabs Solutions for the Expression of Difficult Proteins conference call. (neb.com)
Welcome to everyone who has chosen to join us for this information session on solutions for the expression of difficult proteins. (neb.com)
I will start by outlining the most common problems encountered during the expression of recombinant proteins in E. coli and specific solutions will be given for each problem. (neb.com)
The two most common problems during recombinant protein expression are low expression level and solubility of the target protein. (neb.com)
First let me address the problem of low target protein expression. (neb.com)
Since many of today's expression vectors encode Shine-Dalgarno sequences it is easy to overlook problems with translation initiation. (neb.com)
If you find that the five prime UTR is not optimized for expression then simply change the vector, the ribosome binding site, or adding an affinity tag at the N-terminus often enhances expression. (neb.com)
Finally, low expression may be due to uncontrolled expression of toxic protein during the culture outgrowth phase. (neb.com)
Today we use epigenetics to mean changes to gene expression that are not caused by changes in the actual DNA sequence. (lu.se)
Gene expression is the process of several steps through which the information in a gene's DNA sequence is transferred to the structures and functions of a cell. (lu.se)
Gene expression generally produces proteins, but there are also non-coding genes that produce RNA molecules (rRNA, tRNA). (lu.se)
Transcription factors bind alongside the promoter and set off the gene expression. (lu.se)

Zelda and Bicoid fusion proteins were generated by inserting fluorescent proteins at the endogenous locus. (elifesciences.org)
To avoid potential problems associated with endogenous tagging of a multicopy locus, histone fusions were supplied as exogenous transgenes. (elifesciences.org)
Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis , respectively. (frontiersin.org)

The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. (betalifesci.com)

This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. (wikipedia.org)
Northern blot analysis documented that two transcription factor genes chosen for further study, c-myc promoter-binding protein (MBP-1) and X-box binding protein 1 (XBP-1), were up-regulated in U266 cells about 3-fold relative to the cell cycle-dependent β-actin gene 12 h after IL-6 treatment. (elsevierpure.com)
They are likely to have conserved DNA sequence elements, called transcription factor binding motifs (TFBMs), in their promoter regions. (biomedcentral.com)
For example, our group has examined the genes regulated by the Zap1p transcription factor in S. cerevisiae using DNA microarrays and promoter motif analysis [ 1 ] (Figure 1 ). (biomedcentral.com)
Conserved transcription factor-binding sites within promoter regions of human RON genes were then searched. (spandidos-publications.com)
In front of every gene is a DNA sequence called a promoter. (lu.se)

Mature drying treatment enhanced the ability of 65 DAP peanut embryos to withstand fast drying, also increased the heat stability of arachins, the major peanut storage protein, which was heat labile during 45 to 65 DAP embryogenesis. (jipb.net)
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
It improves protein stability, extends storage time, and reduces shipping costs. (betalifesci.com)
And finally, many proteins from higher organisms require complex disulfide bonds for stability and proper folding. (neb.com)

The role of two members of the ETS (E26 avian leukemia oncogene) family of transcription factors, ETS-1 and ETS-2, has been investigated in many cancers but has not yet been studied in ocular tumors. (molvis.org)
The interlinked phenomena, the team reports, are reflected in a global database of the genes expressed in GBM cells and seen in tumors expressing EGFRvIII obtained from GBM patients. (sciencedaily.com)

A team of Ludwig Cancer Research scientists has mapped out how a mutant version of the epidermal growth factor receptor (EGFR) known as EGFRvIII specifically drives critical processes that alter the reading of the genome to fuel the growth of the brain cancer glioblastoma multiforme and -- most important -- how each process is linked to the other. (sciencedaily.com)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

The mean inoculum density of P.× cambivora in the rhizosphere soil, as determined using the soil dilution plating method and expressed in terms of colony forming units (cfus) per gm of soil, the isolation frequency using leaf baiting, and the percentage of infected fibrous roots from 20 randomly selected beech trees with severe decline symptoms (50 to 100 foliage transparency classes) were 31.7 cfus, 80%, and 48.6%, respectively. (bvsalud.org)
Isolation techniques supplemented by sequencing of DNA from axenic cultures have provided a robust methodology for the study of Phytophthora communities in agricultural and natural ecosystems. (bvsalud.org)
In today's presentation I will describe a new strain that we have engineered to aid the isolation of pure his-tag proteins. (neb.com)

Zinc, a strong Lewis acid (an electron-pair acceptor), is a catalytic component of over 300 enzymes and plays critical structural roles in many proteins, for example in zinc fingers. (biomedcentral.com)
Zap1p activates the transcription of its target genes in zinc-limited but not in zinc-replete yeast cells. (biomedcentral.com)
In this study, we identified zinc finger transcription factor 148 ( ZNF148 , also called Zfp148 and ZBP-89) as a direct target of MYC. (nature.com)

check this if you want to blast your sequences against a large subset of the Swissprot plat proteins. (gabipd.org)
Drosophila HB9 differs from its vertebrate orthologs in several ways: it is not expressed in all somatic motoneurons, it is expressed in a subset of interneurons, and it is required for the proper development of both interneurons and motoneurons. (jneurosci.org)

the mutant protein was found in the cytoplasm, while the wild-type protein was found in the nucleus. (ijbs.com)

while John Weinstein, Chair of the Department of Bioinformatics and Computational Biology at the University of Texas M.D. Anderson Cancer Center, talked on "Personalizing Cancer Medicine in the Era of Next-Generation Sequencing: Omics and Informatics. (biomedcentral.com)

Fibroblast growth factor-18 (Fgf18) was the extracellular and secreted factor that decreased glycosaminoglycan release and depletion from the cartilage, and enhanced proliferation of articular chondrocytes. (cusabio.com)
The extracellular sequences of RON contain several domains including an N-terminal semaphorin (sema) domain, followed by the plexin, semaphorin, integrin (PSI) domain, and four immunoglobulin, plexin, transcription factor (IPT) domains. (spandidos-publications.com)

RON is a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity. (spandidos-publications.com)
Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)

Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). (abcam.com)
V-set and immunoglobulin domain-containing 4 (VSIG4) is a complement receptor of the immunoglobulin superfamily (CRIg) that is specifically expressed in resting tissue-resident macrophages [ 7 , 8 ]. (thno.org)
The targetPROFILER is a profiling assay to monitor the activities of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) under diverse stimuli conditions. (systasy.de)
Recepteur d'origine nantais (RON) is a receptor tyrosine kinase (RTK) normally expressed at low levels in epithelial cells. (spandidos-publications.com)

We developed a high-throughput gene knockout system, and using this system, we obtained null mutants of 104 fungal-specific Zn2Cys6 transcription factor (TF) genes by screening 8741 primary transformants in M. oryzae. (prelekara.sk)

2005) The genome sequence of the rice blast fungus Magnaporthe grisea. (prelekara.sk)
This thematic series of companion papers constitutes the initial exploration of the genome biology of the waterflea (Daphnia) building on the first complete crustacean genome sequence. (biomedcentral.com)

PCR genotyping and DNA sequencing were used to find chromosomes containing tag insertions and to determine the identity of the inserted protein. (elifesciences.org)

The whole coding sequence and nearby 5' untranslated region (UTR) and 3'UTR of the FOXL2 gene were amplified using polymerase chain reaction (PCR) with three sets of overlapping primers, followed by sequencing analyses. (ijbs.com)

Thus, vertebrate HB9/MNR2 genes are expressed specifically in somatic motoneurons and are essential for distinguishing motoneuron/interneuron cell types. (jneurosci.org)
Even if the DNA sequence - the set of genes - is the same in all cells, only certain genes are expressed, i.e. only information from certain genes is copied (transcribed) and used to build protein structures or regulate processes. (lu.se)

Female infertility syndromes are among the most prevalent chronic health disorders in women, but their genetic basis remains unknown because of uncertainty regarding the number and identity of ovarian factors controlling the assembly, preservation, and maturation of ovarian follicles. (appliedbioinfo.com)

Both genes were also similarly up-regulated by IL-6 in factor-dependent ANBL-6 myeloma cells. (elsevierpure.com)
TFs regulate genes to ensure they're expressed in proper sequence and at the right time in cells. (ddw-online.com)
Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. (betalifesci.com)
In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. (betalifesci.com)
RON was found to be primarily expressed in cells of epithelial origin such as colon, breast and skin ( 9 ). (spandidos-publications.com)
These concerted processes change the spectrum of genes expressed by GBM cells. (sciencedaily.com)
Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
The same DNA sequence is then found in all the baby's cells. (lu.se)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

propose a one-dimensional diffusion-reaction model, in the form of a master equation, to analyze the non-equilibrium protein sliding kinetics along a segment of bacterial DNA [ 29 ]. (biomedcentral.com)
Model validity is assessed through Monte Carlo simulations, and the results are interpreted within the context of bacterial transcription. (biomedcentral.com)
This is particularly true when expressing eukaryotic genes in bacterial hosts. (neb.com)

a Domain organization of the four subunits of human Pol δ and amino acid sequence of PCNA-interacting (PIP-box) motifs. (nature.com)
The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. (rndsystems.com)

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. (wikipedia.org)

The N-terminal 6xHis tag sequence was appended to the gene coding for the E.coli of the human FGF18 protein to form the recombinant DNA. (cusabio.com)
And then Mehmet Berkman will highlight the SHuffle line of E. coli strains for producing proteins with complex disulphide bonds. (neb.com)

The MYC proto-oncogene is a transcription factor essential in stem/progenitor cell maintenance and differentiation [ 11 ]. (nature.com)
According to co-senior author Xiaohui Xie, UCI Professor of computer science, the framework enables the study of transcription factor binding at the cellular level, which was previously impossible due to the intrinsic noise and sparsity of single-cell data. (ddw-online.com)
In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. (betalifesci.com)
RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
Notably, silencing of the two transcription factors stopped tumor growth, both in cell cultures and in an animal model that mimics GBM. (sciencedaily.com)
This encoded protein can induce G1 arrest in the cell cycle. (appliedbioinfo.com)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)

Methylation generally serves to suppress gene transcription, meaning that genes with methyl tags are less likely to be expressed. (prohealth.com)
Alternatively, it can also attract proteins that block gene transcription. (prohealth.com)
They are about 6-8 nucleotides (nt) in length and occur upstream of the gene transcription start site (TSS). (biomedcentral.com)

Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)

Complex formation then triggers a signaling cascade that culminates in the transcription of the interferon stimulated genes (ISGs) and additional transcription factors. (rndsystems.com)
It is unknown whether any additional transcription factors promote a general motoneuron identity. (jneurosci.org)

Fgf18 is generally known to activate the IIIc splice variants of FgfR2 and FgfR3, and both of these receptors are known to be expressed in chondrocytes of human and murine articular cartilage. (cusabio.com)

While over half the genes are estimated to be regulated at the transcriptional level, few regulatory motifs and transcription regulators have been found. (biomedcentral.com)
The number of transcription associated proteins (TAPs) encoded by the Pf genome is about one third the number of TAPs associated with the transcriptional process in free-living eukaryotes [ 10 ]. (biomedcentral.com)
The basal transcriptional apparatus (RNA Polymerase II, TATA-box binding protein and other components) in Pf is similar to that in free-living eukaryotes. (biomedcentral.com)
The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). (frontiersin.org)
However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)

Chromatin remodeling complexes and histone acetylases and deacetylases, which modulate the accessibility of DNA to transcription factors, are also present. (biomedcentral.com)

It was concluded that the heat-stable proteins may contribute to desiccation tolerance of the peanut seeds, and the low molecular weight heat-stable polypeptides may confer nonspecifieally heat tolerance on peanut storage proteins which were normally heat labile. (jipb.net)

These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)

Overexpression of OsPIL15 , a phytochrome-interacting factor-like protein gene, represses etiolated seedling growth in rice [J]. J Integr Plant Biol. (jipb.net)
Several well-known oocyte-specific genes were represented, including growth differentiation factor 9, bone morphogenetic protein 15, or the zona pellucida glycoprotein genes. (appliedbioinfo.com)
Estrogens are important regulators of growth and differentiation in (range, 0 -9 fmol/mg protein, median 0.7). (lu.se)

Systematic Analysis of ZnCys Transcription Factors. (prelekara.sk)
Systematic Analysis of ZnCys Transcription Factors Required for Development and Pathogenicity by High-Throughput Gene Knockout in the Rice Blast Fungus. (prelekara.sk)
Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)
Importantly, their approach has the potential to identify transcription factors that might be indirectly regulating the genes and, thus, would not be recognized by sequence-based analysis of the expressed gene promoters. (biomedcentral.com)
The PCR product (584 bp) was subjected to direct sequence analysis, and the identified paramyxovirus was tentatively named ZMLS/2011. (cdc.gov)

Until now, whether this phenotype has a Mendelian inheritance pattern and which underlying genetic factors play a role in its development remained to be elucidated. (appliedbioinfo.com)

Anatomy12

Organisms9

Diseases2

Chemicals and Drugs121

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes79

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science7

Health Care1

Transgenic4

Similarity4

Genomic4

Liquid or Lyophilized1

ESTs5

Histone proteins6

Preferentially expressed4

Purity1

Functional7

Tissues5

Structurally related2

Regulates1

Transcript1

Clone4

Vitro3

Epigenetic5

MRNAs3

Expression32

Endogenous3

Arabidopsis1

Situ1

Promoter6

Stability5

Tumors2

Epidermal growth f1

Recombinant protein1

Isolation3

Zinc3

Subset2

Mutant1

Informatics1

Extracellular2

Heterodimeric2

Receptor4

Mutants1

Genome sequence2

Chromosomes1

Analyses1

Genes are expressed2

Maturation1

Cells9

Bacterial3

Amino acid seq2

Winged helix1

Coli2

Cell7

Gene transcription3

Localization1

Additional transcription factors2

Receptors1

Transcriptional5

Chromatin1

Molecular weight1

Enzymes1

Growth3

Analysis6

Phenotype1