• camelid
  • Those derived from camelid and fish antibodies are less lipophilic and more soluble in water, owing to their complementarity determining region 3 (CDR3), which forms an extended loop (coloured orange in the ribbon diagram above) covering the lipophilic site that normally binds to a light chain. (wikipedia.org)
  • Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. (readbyqxmd.com)
  • Tremendous effort has been expended over the past two and a half decades to understand many aspects of camelid heavy chain antibodies, from their biology, evolution, and immunogenetics to their potential applications in various fields of research and medicine. (readbyqxmd.com)
  • glycoproteins
  • The blocked, immobilized glycoprotein-specific antibodies are able to capture and isolate glycoproteins from a complex sample that is applied directly onto the microarray slides. (jove.com)
  • has recently invented a chemically blocked antibody microarray with a glycan-binding protein (GBP) detection method for high-throughput and multiplexed profile glycosylation of native glycoproteins in a complex sample 18 . (jove.com)
  • Antibodies are highly functional glycoproteins capable of providing immune protection through multiple mechanisms, including direct pathogen neutralization and the engagement of their Fc-portions with surrounding effector immune cells and immune components that induce anti-pathogenic responses. (readbyqxmd.com)
  • Amyloid
  • In addition we are collaborating with various groups to identify other single domains from our libraries against other targets of interest including Amyloid protein, which is involved in Alzheimer's disease. (edu.au)
  • Patients with Alzheimer's disease suffer from synaptic and neuronal loss, which is thought to be caused by the presence of a chemically "sticky" protein called amyloid beta (Aβ). (allelebiotech.com)
  • Mutations in the TTR gene cause misfolding of the protein and the formation of amyloid fibrils that typically contain both mutant and wild-type TTR that deposit in tissues such as the peripheral nerves and heart, resulting in intractable peripheral sensory neuropathy, autonomic neuropathy, and/or cardiomyopathy. (pharmaceuticalintelligence.com)
  • functional
  • Small modifications to multiple antibody biophysical features induced by vaccines and other therapeutic regimens can significantly alter functional immune outcomes, though it is difficult to predict which combinations confer protective immunity. (readbyqxmd.com)
  • proteins
  • Shark antibodies and their human equivalents are small and extremely stable with long loops that are able to penetrate into important regions of proteins. (edu.au)
  • In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. (jove.com)
  • Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed. (jove.com)
  • Introduction Glycosylation of protein, which is the most ubiquitous post-translational modification on proteins, modifies the physical, chemical, and biological properties of a protein, and plays a fundamental role in various biological processes 1-6 . (jove.com)
  • The resulting stabilized beta-catenin enters the nucleus and activates transcription of Wnt target genes through its interactions with Tcf-family transcription factors, proteins that contain a beta-catenin-binding domain and a sequence-specific DNA-binding domain. (stanford.edu)
  • amino acids
  • 4,5 Much recent work has focused on the distribution of amino acids preferentially found in the membrane environment in comparison to aqueous solution and the different intermolecular forces that drive protein association. (jove.com)
  • Interestingly, the same region of NS1, which comprises amino acids 224HWPKPHTLW232, was conserved for both kinds of antibodies displaying the consensus motif histidine-tryptophan-tryptophan or tryptophan-proline-tryptophan. (jove.com)
  • novel
  • Sequence analysis of the VHH antibodies revealed novel and long complementarity determining regions 3 (CDR3). (jove.com)
  • resistance
  • Once activated, the drug sets in motion the destruction of a resistance protein that repairs certain DNA lesions. (bio-medicine.org)
  • Bispecific antibodies that can inhibit two correlated signaling molecules at the same time can be developed to overcome inherent or acquired resistance and to be more efficient angiogenesis inhibitors. (mdpi.com)
  • gene
  • Pseudomonas aeruginosa exoS gene contains important replacement (non-synonymous) single-nucleotide polymorphism (SNP) loci, of which mutations in loci 162 (G162A) and 434 (G434C) in exoS greatly affects virulence. (readbyqxmd.com)
  • receptor
  • WHO sets a goal to have universal vaccine by 2020, it seems we are close to get it done within five-year timeframe as indicated in a recent article in PNAS on "broadly neutralizing antibody" and stimulating cellular responses though Fc receptor interactions. (genscript.com)
  • Infection
  • Infection specificity is achieved by substituting a chimeric G, composed of the extracellular domain of the ASLV-A glycoprotein and the cytoplasmic domain of the RABV-G (A/RG), for the normal RABV-G 1 . (jove.com)
  • This demonstrates that VHH antibodies are likely to be an option for developing point-of-care tests against DENV infection. (jove.com)
  • chimeric
  • 9 The transmembrane domain of interest is expressed as a chimeric protein with maltose binding protein for location to the periplasm and ToxR to provide a report of the level of oligomerization (Figure 1). (jove.com)
  • typically
  • However, current methods to study protein glycosylation typically are too complicated or expensive for use in most normal laboratory or clinical settings and a more practical method to study protein glycosylation is needed. (jove.com)
  • Next-generation DNA sequencing (NGS) technologies have now made it possible to interrogate antibody repertoires to unprecedented depths, typically by sequencing of cDNAs encoding immunoglobulin variable domains. (readbyqxmd.com)
  • suitable
  • The final step is the translation of the optimised single-domain antibody in E. coli, Saccharomyces cerevisiae or other suitable organisms. (wikipedia.org)
  • The wavelength is suitable for the range of 400 to 700 nm, and protein concentrations (human cancer biomarkers) can be measured accurately. (genscript.com)
  • However, methods to study protein glycosylation have been complicated, and not suitable for routine laboratory and clinical settings. (jove.com)
  • multiple
  • In this method, multiple immobilized glycoprotein-specific antibodies are printed directly onto the microarray slides and the N-glycans on the antibodies are blocked. (jove.com)
  • cancer
  • Monoclonal antibodies have revolutionized cancer therapy. (readbyqxmd.com)
  • Immunogenetic analyses of expressed antibody repertoires are becoming increasingly common experimental investigations and are critical to furthering our understanding of autoimmunity, infectious disease, and cancer. (readbyqxmd.com)
  • The study funded by the National Institutes of Health (NIH) is the next step in developing targeted cancer therapies, said the lead researcher, Peter Glazer, M.D., chair of therapeutic radiology and leader of the radiobiology research program at Yale Cancer Center. (bio-medicine.org)
  • We feel this could create an Achilles heel for cancer cells that would make them more vulnerable to traditional cancer therapies. (bio-medicine.org)
  • Besides retargeting effector cells to the site of cancer, new applications were established for bispecific antibodies. (mdpi.com)
  • complexes
  • We also present carefully optimized procedures for expression and purification that are equally applicable whether producing single TM domains or crosslinked complexes and provide advice for adapting these methods to new TM sequences. (jove.com)
  • Human INO80 complexes consist of 14 protein subunits including Ino80, a SNF2-like ATPase, which serves both as the catalytic subunit and the scaffold for assembly of the complexes. (jove.com)
  • clinical
  • Since NNTTR is pathogenic in ATTR amyloidosis and the level of NNTTR reduction correlated with total TTR knockdown, these results provide direct mechanistic evidence supporting the therapeutic hypothesis that TTR knockdown has the potential to result in clinical benefit. (pharmaceuticalintelligence.com)
  • An increasing number of monoclonal antibodies have obtained approval and entered clinical applications [ 1 ]. (mdpi.com)
  • study
  • We reported data from our ongoing Phase 2 open-label extension (OLE) study of patisiran, an investigational RNAi therapeutic targeting transthyretin (TTR) for the treatment of TTR-mediated amyloidosis (ATTR amyloidosis) patients with familial amyloidotic polyneuropathy (FAP). (pharmaceuticalintelligence.com)
  • potential
  • The therapeutic potential of anti-rabies VHH was examined in a mouse model using intranasal challenge with a lethal dose of rabies virus. (jove.com)