• camelid
  • Those derived from camelid and fish antibodies are less lipophilic and more soluble in water, owing to their complementarity determining region 3 (CDR3), which forms an extended loop (coloured orange in the ribbon diagram above) covering the lipophilic site that normally binds to a light chain. (wikipedia.org)
  • Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. (readbyqxmd.com)
  • Tremendous effort has been expended over the past two and a half decades to understand many aspects of camelid heavy chain antibodies, from their biology, evolution, and immunogenetics to their potential applications in various fields of research and medicine. (readbyqxmd.com)
  • peptide
  • A single-domain antibody is a peptide chain of about 110 amino acids long, comprising one variable domain (VH) of a heavy-chain antibody, or of a common IgG. (wikipedia.org)
  • They are collaborating to genetically modify human neural stem cells to express a small (58-amino acid) peptide derived from the protein called α1-takusan, which Scintillon researchers previously discovered to harbor a protective activity against Aβ-induced toxicity (1) . (allelebiotech.com)
  • molecular
  • research includes protein-drug interaction, molecular synthesis, and surface and material science. (wikipedia.org)
  • 6,7 Nevertheless, studies of molecular recognition at the transmembrane domain of proteins still lags behind those of water-soluble regions. (jove.com)
  • Patrick Sung, professor of therapeutic radiology and of molecular biophysics and biochemistry, will focus on the repair genes BRCA2, FANCD2, and RAD51, and how their repair pathways are regulated at the level of protein-protein interactions. (bio-medicine.org)
  • With the development of molecular cloning technology and the deep understanding of antibody engineering, there are diverse bispecific antibody formats from which to choose to pursue the optimal biological activity and clinical purpose. (mdpi.com)
  • functional
  • Antibodies are highly functional glycoproteins capable of providing immune protection through multiple mechanisms, including direct pathogen neutralization and the engagement of their Fc-portions with surrounding effector immune cells and immune components that induce anti-pathogenic responses. (readbyqxmd.com)
  • Small modifications to multiple antibody biophysical features induced by vaccines and other therapeutic regimens can significantly alter functional immune outcomes, though it is difficult to predict which combinations confer protective immunity. (readbyqxmd.com)
  • Amyloid
  • In addition we are collaborating with various groups to identify other single domains from our libraries against other targets of interest including Amyloid protein, which is involved in Alzheimer's disease. (edu.au)
  • Patients with Alzheimer's disease suffer from synaptic and neuronal loss, which is thought to be caused by the presence of a chemically "sticky" protein called amyloid beta (Aβ). (allelebiotech.com)
  • Mutations in the TTR gene cause misfolding of the protein and the formation of amyloid fibrils that typically contain both mutant and wild-type TTR that deposit in tissues such as the peripheral nerves and heart, resulting in intractable peripheral sensory neuropathy, autonomic neuropathy, and/or cardiomyopathy. (pharmaceuticalintelligence.com)
  • therapeutics
  • Our group is using single domain antibodies that have been developed from sharks to identify novel therapeutics against a number of chronic diseases. (edu.au)
  • Research groups in the institute have multiple collaborations and links to industry, and the institute hosts two biotechnology companies: AdAlta Pty Ltd and Hexima Ltd. AdAlta develops single-domain antibodies for use as therapeutics and diagnostics. (wikipedia.org)
  • Our ATTR program is the lead effort in our Genetic Medicine Strategic Therapeutic Area (STAr) product development and commercialization strategy, which is focused on advancing innovative RNAi therapeutics toward genetically defined targets for the treatment of rare diseases with high unmet medical need. (pharmaceuticalintelligence.com)
  • ProSci's rabbit monoclonal antibodies continue to exceed client expectations with wide applicability including diagnostics, therapeutics, and ELISA. (prosci-inc.com)
  • molecules
  • Bispecific antibodies that can inhibit two correlated signaling molecules at the same time can be developed to overcome inherent or acquired resistance and to be more efficient angiogenesis inhibitors. (mdpi.com)
  • development
  • Our single domain antibody against CXCR4 was found to block HIV invasion as well as being able to reduce inflammation and stop the development of fibrosis in mouse models of lung fibrosis and eye fibrosis. (edu.au)
  • Creative Biolabs offers a comprehensive platform for antibody discovery and development. (creative-biolabs.com)
  • Relying on the remarkable academic team and profound expertise, Creative Biolabs has established custom anti-venom antibody development service that can meet our global clients' specific requirements. (creative-biolabs.com)
  • However, the high cost of generating therapeutic horse sera and detrimental side effects limit the development of anti-venom antibodies. (creative-biolabs.com)
  • Fig.1 The Basic process of anti-venom antibody development. (creative-biolabs.com)
  • Creative Biolabs has been a long-term expert in the field of anti-venom antibody development. (creative-biolabs.com)
  • If you are interested in our anti-venom antibody discovery and development service, please do not hesitate to inquire us for more details. (creative-biolabs.com)
  • Some species have been shown to be active in the intestine after oral application, but their low absorption from the gut impedes the development of systemically active orally administered single-domain antibodies. (wikipedia.org)
  • the first being the creation of nanoantibodies against Aβ, which has generated multiple single-domain antibodies now in early development. (allelebiotech.com)
  • This regulatory role has led to the development of monoclonal antibodies (mAbs) designed to block CTLA‑4 activity for enhancing immune responses against cancer. (readbyqxmd.com)
  • The development of these specialized antibodies can help researchers avoid common challenges associated with low affinity and non specific antibodies. (prosci-inc.com)
  • Traditional direct B cell cloning preserves the original heavy and light chain antibody pairs, but shares a limitation with the other: they use the entire pool of B cells for development, ranging from newly differentiated B cells all the way through fully differentiated B cells, also known as plasma cells. (prosci-inc.com)
  • ProSci's Plasma Cell Technology 2 isolates plasma cells at the beginning and uses 100% plasma cells as the starting material for antibody development. (prosci-inc.com)
  • amino acids
  • 4,5 Much recent work has focused on the distribution of amino acids preferentially found in the membrane environment in comparison to aqueous solution and the different intermolecular forces that drive protein association. (jove.com)
  • Interestingly, the same region of NS1, which comprises amino acids 224HWPKPHTLW232, was conserved for both kinds of antibodies displaying the consensus motif histidine-tryptophan-tryptophan or tryptophan-proline-tryptophan. (jove.com)
  • novel
  • Sequence analysis of the VHH antibodies revealed novel and long complementarity determining regions 3 (CDR3). (jove.com)
  • minimal
  • The minimal target recognition module of a conventional antibody is composed of two non-covalently associated variable domains (VH and VL). (readbyqxmd.com)
  • gene
  • Pseudomonas aeruginosa exoS gene contains important replacement (non-synonymous) single-nucleotide polymorphism (SNP) loci, of which mutations in loci 162 (G162A) and 434 (G434C) in exoS greatly affects virulence. (readbyqxmd.com)
  • receptor
  • WHO sets a goal to have universal vaccine by 2020, it seems we are close to get it done within five-year timeframe as indicated in a recent article in PNAS on "broadly neutralizing antibody" and stimulating cellular responses though Fc receptor interactions. (genscript.com)
  • Infection
  • Infection specificity is achieved by substituting a chimeric G, composed of the extracellular domain of the ASLV-A glycoprotein and the cytoplasmic domain of the RABV-G (A/RG), for the normal RABV-G 1 . (jove.com)
  • This demonstrates that VHH antibodies are likely to be an option for developing point-of-care tests against DENV infection. (jove.com)
  • proteins
  • Shark antibodies and their human equivalents are small and extremely stable with long loops that are able to penetrate into important regions of proteins. (edu.au)
  • In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. (jove.com)
  • Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed. (jove.com)
  • Introduction Glycosylation of protein, which is the most ubiquitous post-translational modification on proteins, modifies the physical, chemical, and biological properties of a protein, and plays a fundamental role in various biological processes 1-6 . (jove.com)
  • purification
  • We also present carefully optimized procedures for expression and purification that are equally applicable whether producing single TM domains or crosslinked complexes and provide advice for adapting these methods to new TM sequences. (jove.com)
  • expression
  • Creative Biolabs can offer advanced protein engineering platform for your specific project, including high-scale expression, crystallization and characterization. (creative-biolabs.com)
  • 2. The method of claim 1, further comprising expressing the target-specific polyclonal antibody population in an expression vector. (patentsencyclopedia.com)
  • chimeric
  • 9 The transmembrane domain of interest is expressed as a chimeric protein with maltose binding protein for location to the periplasm and ToxR to provide a report of the level of oligomerization (Figure 1). (jove.com)
  • typically
  • However, current methods to study protein glycosylation typically are too complicated or expensive for use in most normal laboratory or clinical settings and a more practical method to study protein glycosylation is needed. (jove.com)
  • Next-generation DNA sequencing (NGS) technologies have now made it possible to interrogate antibody repertoires to unprecedented depths, typically by sequencing of cDNAs encoding immunoglobulin variable domains. (readbyqxmd.com)
  • increasingly
  • Immunogenetic analyses of expressed antibody repertoires are becoming increasingly common experimental investigations and are critical to furthering our understanding of autoimmunity, infectious disease, and cancer. (readbyqxmd.com)
  • suitable
  • The final step is the translation of the optimised single-domain antibody in E. coli, Saccharomyces cerevisiae or other suitable organisms. (wikipedia.org)
  • The wavelength is suitable for the range of 400 to 700 nm, and protein concentrations (human cancer biomarkers) can be measured accurately. (genscript.com)
  • However, methods to study protein glycosylation have been complicated, and not suitable for routine laboratory and clinical settings. (jove.com)
  • bone
  • Bispecific antibodies also have broad application prospects in bone disorders and infections and diseases of the central nervous system. (mdpi.com)
  • cancer
  • Monoclonal antibodies have revolutionized cancer therapy. (readbyqxmd.com)
  • The study funded by the National Institutes of Health (NIH) is the next step in developing targeted cancer therapies, said the lead researcher, Peter Glazer, M.D., chair of therapeutic radiology and leader of the radiobiology research program at Yale Cancer Center. (bio-medicine.org)
  • We feel this could create an Achilles heel for cancer cells that would make them more vulnerable to traditional cancer therapies. (bio-medicine.org)
  • Besides retargeting effector cells to the site of cancer, new applications were established for bispecific antibodies. (mdpi.com)
  • complexes
  • Human INO80 complexes consist of 14 protein subunits including Ino80, a SNF2-like ATPase, which serves both as the catalytic subunit and the scaffold for assembly of the complexes. (jove.com)
  • large
  • This leads to a wide concept for the construction of diverse bispecific antibodies, and thus, a large number of technologies for constructing bispecific antibodies have emerged. (mdpi.com)