AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Chlamydia trachomatisChlamydia InfectionsDNA ProbesDNA, BacterialPolymerase Chain ReactionLymphogranuloma VenereumFemale Urogenital DiseasesNucleic Acid HybridizationMale Urogenital DiseasesGenital Diseases, FemaleGonorrheaCervix UteriUterine CervicitisUrethraPelvic Inflammatory DiseaseTrachomaMolecular ProbesNeisseria gonorrhoeaeChlamydiaSalpingitisBase SequenceOligonucleotide ProbesSensitivity and SpecificityNucleic Acid Amplification TechniquesMolecular Sequence DataChlamydophila psittaciUterine Cervical DiseasesBacterial Outer Membrane ProteinsSexually Transmitted Diseases, BacterialUrineAntibodies, BacterialFluorescent Antibody Technique, DirectEvaluation Studies as TopicReagent Kits, DiagnosticBacteriological TechniquesConjunctivitis, InclusionDNA Probes, HLAUrethral DiseasesPorinsInclusion BodiesMolecular Probe TechniquesSpecimen HandlingDigoxigeninUrogenital SystemBlotting, SouthernCloning, MolecularPolymorphism, Restriction Fragment LengthReproductive Tract InfectionsGenes, BacterialIn Situ Hybridization, FluorescenceSpecies SpecificityFallopian Tube DiseasesAntigens, BacterialMycoplasma genitaliumHeLa CellsArthritis, ReactiveGenital Diseases, MaleAzithromycinChlamydophila pneumoniaeGenitalia, FemaleVaginal DiseasesBacterial Proteins
ChlamydiaNeisseriaPlasmidInfectionBacteriumInfectionsStrainsOligonucleotideAssayConjunctivitisGenomicREAL-TIMNucleicDetectionPrevalenceGonorrhoeaeBacteriaClinicalPolymerase chain reComplementary to the targetInfectiousExtrachromosomalDiseasesAmplificationReagentsProteinsPresenceAmplifyInhibitorsVaginal swabRecombinantYoung adultsBlotHumansPrimersInclusionSequenceBindGuidelinesEnzymeTargetOrganismsMastermixControl
Chlamydia41
Neisseria1
  • The Neisseria gonorrhoeae assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of a specific target nucleic acid sequence in the Opa gene of Neisseria gonorrhoeae. (cdc.gov)
Plasmid3
  • In addition to the chromosome that contains most of the genome, nearly all C. trachomatis strains carry a 7.5 kilobase plasmid that contains 8 genes. (wikipedia.org)
  • The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific target sequence within the C. trachomatis plasmid DNA. (cdc.gov)
  • Most C. trachomatis strains have a multicopy extrachromosomal cryptic plasmid, which is widely used as the target for PCR testing. (roboscreen.com)
Infection4
  • T of C. trachomatis infection blunts the immune response, rachoma was first recognized as an ocular disease in the increasing the patient's susceptibility to reinfection ( 4 ). (cdc.gov)
  • C trachomatis infection causes other diseases as well, including conjunctivitis, pneumonia or pneumonitis, afebrile pneumonia syndrome (in infants born vaginally to infected mothers), Fitz-Hugh-Curtis syndrome, and trachoma (the world's leading cause of acquired blindness). (medscape.com)
  • Screening programs have been demonstrated to reduce both the prevalence of C. trachomatis infection and rates of PID in women (79,80). (cdc.gov)
  • In men, the most common manifestation of C. trachomatis infection is inflammation of the urethra (about 50% of cases) [4]. (roboscreen.com)
Bacterium1
  • C. trachomatis is the most common infectious cause of blindness and the most common sexually transmitted bacterium. (wikipedia.org)
Infections4
  • To eliminate infections with species trachoma was spread from the Middle East to Europe during other than C. trachomatis, longer treatment intervals might the Crusades and was a major cause of blindness during the be required ( 8 ). (cdc.gov)
  • trachomatis infections are the leading cause of sexually transmitted diseases in the United States. (ammoliteprodukties.nl)
  • The young women have the highest prevalence of genitourinary C. trachomatis infections. (roboscreen.com)
  • Neonates born of infected mothers can develop inclusion conjunctivitis, nasopharyngeal infections and pneumonia due to C. trachomatis [5]. (roboscreen.com)
Strains2
  • C. trachomatis strains are generally divided into three biovars based on the type of disease they cause. (wikipedia.org)
  • C trachomatis can be differentiated into 18 serovars (serologically variant strains) on the basis of monoclonal antibody-based typing assays. (medscape.com)
Oligonucleotide2
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • The resulting liposome-target complexes will be subsequently magnetically immobilized within the microfluidic channels of the CARD by use of magnetic beads conjugated to other oligonucleotide probes that are complementary to another region of the target sequences. (genomeweb.com)
Assay5
  • If you select the control assay, then the control primers and probe will be mixed with the pathogen primers and probe. (pcrassays.com)
  • The Synthetic DNA positive control contains a mixture of 500 bp regions of all the pathogens and the control assay at approximately 5000 copies/uL for each target. (pcrassays.com)
  • Also note that the synthetic DNA control is specific for each assay, and thus the positive control cannot be used to test other assays that are directed to other regions of the genome. (pcrassays.com)
  • The assay was demonstrated to be specific for H. pylori with a lower limit of detection of 100 fg of bacterial genomic DNA. (microbiologyresearch.org)
  • The Abbott RealTime HBV is an in vitro polymerase chain reaction (PCR) assay for the quantitation of Hepatitis B Virus (HBV) DNA in human plasma or serum from HBV-infected individuals. (dssimage.com)
Conjunctivitis1
  • C. trachomatis is the single most important infectious agent associated with blindness (trachoma), and it also affects the eyes in the form of inclusion conjunctivitis and is responsible for about 19% of adult cases of conjunctivitis. (wikipedia.org)
Genomic3
  • Using genomic DNA as common reference in microarray experiments has recently been tested by different laboratories. (biomedcentral.com)
  • Pair-wise comparison of three experimental conditions was obtained either with two labeled cDNA samples co-hybridized to the same array, or by employing Shewanella genomic DNA as a standard reference. (biomedcentral.com)
  • This work could serve as a guideline for microarray data analysis using genomic DNA as a standard reference. (biomedcentral.com)
REAL-TIM1
  • DNA Probe/Multiplex Real Time PCR for Vaginitis which detects 3 common pathogens- Gardnerella vaginalis, Candida albicans and Trichomonas vaginalis. (shimclinic.com)
Nucleic3
  • 15. In the case of tests for infectious agents, methodologies include detection by immunofluorescence, immunoassay, or nucleic acid probe techniques. (codemap.com)
  • The template probe is composed of the complementary strand of the target and two free nucleic acid anchors at the head and tail. (bvsalud.org)
  • The IC nucleic acids contain primer binding regions identical to those of the target sequence and contain a unique probe binding region that differentiates the IC from amplified target nucleic acid. (arabslab.com)
Detection3
  • The detection of specific DNA sequences and the identification of single nucleotide polymorphisms are important for disease diagnosis. (bvsalud.org)
  • Herein, by combining the high specificity of the base-stacking effect with the high reproducibility of bovine serum albumin (BSA) modified electrodes and the high loading performance of DNA nanoclews (DNA NCs), a novel sandwich-type electrochemiluminescence (ECL) biosensor is reported for the highly specific detection of HPV16 (chosen as the model target). (bvsalud.org)
  • The CMV PCR Kit constitutes a ready-to use system for the detection of CMV DNA using polymerase chain reaction (PCR). (dssimage.com)
Prevalence2
  • Although there are no recommendations to screen heterosexual men, it USPSTF suggests testing to test sexually active heterosexual men in clinical settings with a high prevalence of C. trachomatis (e.g. (cdc.gov)
  • If shown to be effective, the widespread use of mouthwash will reduce the prevalence of oropharyngeal gonorrhoea, which plays key role in driving the emergence of gonococcal antimicrobial resistance through DNA exchange with oral commensal bacteria. (biomedcentral.com)
Gonorrhoeae2
  • C. trachomatis and N. gonorrhoeae control efforts in men differ substantially from those recommended for women. (cdc.gov)
  • And earlier this month, at the American Association for Clinical Chemistry annual meeting in Anaheim, Calif., Rheonix presented data from research studies demonstrating the use of the CARD system for detecting in human subjects warfarin sensitivity, human papillomavirus, and four sexually transmitted infectious agents - N. gonorrhoeae , C. trachomatis , T. palladium , and T. vaginalis - either singly or in multiplex ( PCR Insider , 8/5/10 ). (genomeweb.com)
Bacteria3
  • Over the course of the C. trachomatis life cycle, the bacteria take on two distinct forms. (wikipedia.org)
  • The C. trachomatis genome is substantially smaller than that of many other bacteria at approximately 1.04 megabases, encoding approximately 900 genes. (wikipedia.org)
  • PNAS publishes a paper by Stanley Cohen and Herbert Boyer in which they demonstrate the expression of a foreign gene implanted in bacteria by recombinant DNA methods. (ebioworld.com)
Clinical1
  • Specific DNA present in small amounts in a clinical specimen are amplified by PCR so they become detectable. (medscape.com)
Polymerase chain re1
Complementary to the target2
  • The oligonuleotides are designed to be complementary to the target sequence so that in the presence of target, the probes will bind adjacent to one another. (cdc.gov)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
Infectious1
  • Using one strand of natural viral DNA to assemble 5,300 nucleotide building blocks, Nobel Laureate Arthur Kornberg's Stanford group synthesizes infectious viral DNA. (ebioworld.com)
Extrachromosomal1
  • Instability of extrachromosomal DNA transformed into the diatom Phaeodactylum tricornutum. (harvard.edu)
Diseases1
  • Different types of C. trachomatis cause different diseases. (wikipedia.org)
Amplification1
  • 2 Diagnostic tests based on DNA amplification have made outreach sampling possible, 3 and several studies have shown the feasibility of applying such test strategies in the field, 4 in military settings, 5 and in schools. (bmj.com)
Reagents1
  • Contamination of reagents or specimens with target DNA from the environment must be avoided because such contamination can produce false-positive results. (medscape.com)
Proteins3
  • The RB synthesizes its own DNA, RNA, and proteins but requires energy in the form of adenosine triphosphate (ATP) from the host cell. (medscape.com)
  • Reversible phosphorylation of nuclear proteins is required for both DNA replication and entry into mitosis. (duke.edu)
  • Currently, ongoing projects include the elucidation of the functions of L. pneumophila effector proteins that are involved in actin dynamics or are targeted to the nucleus, as well as a C. trachomatis Golgi associated protein. (unl.pt)
Presence1
  • In the presence of the target DNA, the template probes can form stacked base pairs with target, generating high base-stacking energy. (bvsalud.org)
Amplify2
  • In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
  • Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products was confirmed by Southern blot hybridisation to a digoxigenin-labelled H. pylori probe. (microbiologyresearch.org)
Inhibitors1
  • Radiolabeled inhibitors as probes for imaging mutant IDH1 expression in gliomas: Synthesis and preliminary evaluation of labeled butyl-phenyl sulfonamide analogs. (duke.edu)
Vaginal swab1
  • Patient self-collection: Partially open the package of the Gen-Probe® Aptima® vaginal swab kit. (ammoliteprodukties.nl)
Recombinant3
  • After two years of discussion between Stanford and the NIH, the federal government affirms that universities can hold patents and license recombinant DNA inventions. (ebioworld.com)
  • Genentech Inc. And The City of Hope National Medical Center announce the successful laboratory production of human insulin using recombinant DNA technology. (ebioworld.com)
  • Preventing packaging of translatable P5-associated DNA contaminants in recombinant AAV vector preps. (harvard.edu)
Young adults1
  • The aim of this study was to assess the effectiveness of a mass media campaign to recruit young adults for C trachomatis testing by use of home obtained mailed samples. (bmj.com)
Blot1
  • The amplified segment may be observed using electrophoretic gel or can be identified by Southern blot analysis, using specific DNA probes for that segment. (medscape.com)
Humans2
  • C. trachomatis along with C. pneumoniae have been found to infect humans to a greater extent. (wikipedia.org)
  • C. trachomatis exclusively infects humans. (wikipedia.org)
Primers3
  • In the second step, the temperature decreases (45-60°C), depending on the primers, to permit annealing (strengthening) of the target DNA primers. (medscape.com)
  • One kit provides enough primers and probes to perform 100 PCR reactions with 20 uL total volume. (pcrassays.com)
  • The Enzyme mastermix is provided in a separate tube from the primers and probes. (pcrassays.com)
Inclusion1
  • Within the inclusion, C. trachomatis transforms into a larger, more metabolically active form called the reticulate body. (wikipedia.org)
Sequence1
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
Bind1
  • This results in the shorter free anchors of template probes being able to bind to the capture and signal probes. (bvsalud.org)
Guidelines1
  • Science publishes a letter by Stanford Biochemist Paul Berg and others calling for National Institute of Health Guidelines for DNA Splicing. (ebioworld.com)
Enzyme1
  • Cohen and Boyer show that DNA can be cut with a restriction enzyme, joined together with other enzymes, and reproduced by inserting the DNA into Escherichia coli. (ebioworld.com)
Target4
  • The temperature of these is increased to 90-95°C in order to separate (denature) the 2 strands of target DNA. (medscape.com)
  • The target DNA segment is amplified in the range of 10 5 - to 10 6 -fold by repeating this cycle no less than 30-40 times. (medscape.com)
  • This will be accomplished by conjugating oliogonucleotide probes that are complementary to portions of the amplified target sequences, to the exterior surface of liposomes which, in turn, will encapsulate electroactive compounds," the grant's abstract states. (genomeweb.com)
  • The various immunotherapy types include DNA or RNA-based vaccines, protein-based vaccines, dendritic cell vaccines, and TIL-based therapies that target various disease indications such as colorectal cancer, renal cell carcinoma, non-small cell lung cancer, bone cancer, and gynecological cancer. (thebusinessresearchcompany.com)
Organisms1
  • Oswald Avery, Colin Macleod and Maclyn McCarty demonstrate that DNA, not protein, is the hereditary material in most living organisms. (ebioworld.com)
Mastermix1
  • 50 μl of extracted DNA have to be pipetted into the tube and the ready mastermix is diluted. (roboscreen.com)
Control1
  • When using DNA extraction kits of other manufacturers, it is highly recommended to use Internal Control sample (IC) (VBC8881, BIORON Diagnostics). (roboscreen.com)