Toxicity biological assay. Medical search. Frequent questions

Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
My research focuses on comprehensive evaluation of in vitro assays to improve the repeatability and interlaboratory agreement of the assay results. (nist.gov)
Overall, these efforts will help create a process for increasing measurement assurance for in vitro assays, facilitating their standardization and their use in adverse outcome pathway models. (nist.gov)
in vivo) activity per unit particle surface area showed that all in vitro assays correlated significantly with in vivo results, with the cellular assays correlating the best. (cdc.gov)

Experimental evidence suggests that anhydrotetrodotoxin does not possess sufficient acute toxicity to pose a significant public health threat. (selectagents.gov)
Then the Safflower ointment was manufactured and its acute toxicity study on rats was tested. (techscience.com)
The prepared ointment had an excellent acute toxicity safety profile. (techscience.com)
Finally, oral acute toxicity at a dose of 2000 mg/kg was done according to the Organization for Economic Co-operation and Development guideline n° 423. (biomedcentral.com)
In the acute toxicity evaluation, all animals were physically active and no deaths of rats were observed during the test. (biomedcentral.com)
Acute toxicity studies using rodents were used to calculate an acceptable daily intake (ADI) value of 20 mg·kg −1 body weight per day. (ashs.org)

Engineering spatial-organized cardiac organoids for developmental toxicity testing. (harvard.edu)
Toxicity profiling of flame retardants in zebrafish embryos using a battery of assays for developmental toxicity, neurotoxicity, cardiotoxicity and hepatotoxicity toward human relevance. (research.com)

These findings suggest quantitative considerations for building scientific confidence in NAM-based systemic toxicity predictions. (nih.gov)
The clinical application of gambogic acid, a natural component with promising antitumor activity, is limited due to its extremely poor aqueous solubility, short half-life in blood, and severe systemic toxicity. (dovepress.com)
For this reason, the skin is often exposed to potentially hazardous agents, including chemicals, which may contribute to the onset of a spectrum of adverse health effects ranging from localized damage (e.g., irritant contact dermatitis and corrosion) to induction of immune-mediated responses (e.g., allergic contact dermatitis and pulmonary responses), or systemic toxicity (e.g., neurotoxicity and hepatoxicity). (cdc.gov)
4. Subjects who have NOT received prior systemic therapy (including cytotoxic chemotherapy, targeted therapy, or antineoplastic biological therapy) for their metastatic NSCLC. (who.int)

In vitro and acellular assays can play an important role in determining the potential toxicity of compounds to humans and ecological receptors. (nist.gov)
Collaborations with other departments are of paramount importance since chemists are not able to perform most of the biological assays required to determine the efficacy of the synthesised compounds. (asynt.com)
Biologists can determine the activity, toxicity, bioavailability and other pharmacological properties of the synthesised compounds. (asynt.com)
In the research described in the paper for example, we have been able to not only synthesise a range of novel quinoxaline-alkynyl derivatives but then also carry out in-vitro testing to investigate the biological activity of these compounds. (asynt.com)
Although I no longer spend my days in a lab, as a medicinal chemist myself, I always found it highly motivating to almost see the fruits of your labour - synthesising novel compounds and then actually seeing them have an effect against a certain biological target is hugely rewarding. (asynt.com)
All the newly synthesized compounds were screened for their antioxidant activity by 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radical scavenging assay method. (ijpsonline.com)
In addition, the checkerboard assay that tests the association of compounds, showed a synergistic effect, suggesting a potentiation of the antibacterial effect when porphyrin was tested in combination with ciprofloxacin and vancomycin. (bvsalud.org)
These requirements may need simple assay development, primarily focusing on screening and assessing lead compounds. (uscalifornia.com)
Furthermore, no toxicity was observed from the compounds in a wax moth larvae model, establishing a platform for the development of a series of new antibacterial drugs with an established mode of action. (edu.au)

Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Toxicity Tests" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)

The antimicrobial activity assays were performed at specific photosensitizer (PS) concentrations under dark and white-light LED irradiation conditions for 120 min. (bvsalud.org)

Isatin (1-H-indole-2,3- dione) and its derivatives possess diverse biological and pharmacological activities and are widely used as a starting material for the synthesis of a broad range of heterocyclic compound substrates for drug synthesis [ 1 ]. (ijpsonline.com)
Synthesis, in vitro transcription inhibition assays, and biological testing of the hybrids identified a range of potent anti-transcription inhibitors with activity against a range of pathogenic bacteria with MICs as low as 3.1 μM. (edu.au)

The proposed platform allows characterization of hundreds of protein sequences at significant cost and time savings by providing a combined ex vivo computational, expression, and assay system. (sbir.gov)
The proposed platform is an all-encompassing ex-vivo computational, expression, and assay system. (sbir.gov)
Richard S. Paules focuses on Toxicity, Computational biology, Function, Prioritization and High-Throughput Screening Assays. (research.com)

In DPPH radical scavenging assay, the effect on reducing free radicals increased in a dose dependent manner. (biomedcentral.com)
SOR), hydrogen peroxide, and 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) radical activity by a model (most biological y active) of the anticancer plant was also evaluated. (who.int)

Endpoints in all assays were related to oxidative stress and responses were expressed per unit NP surface area to compare the results of different assays. (cdc.gov)

This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)
Although the toxicity values determined for I. obsoleta exceeded irgarol concentrations measured in surface waters, results from this toxicity assessment will provide valuable information to environmental resource managers faced with decisions regarding the use and regulation of antifoulant paints in the coastal zone. (unboundmedicine.com)

During the drug development phase, the entire focus shifts to assessing the efficacy, toxicity, and safety profile of a new drug molecule. (uscalifornia.com)

The knowledge gained in this project on the transport and transformation routes and toxicity of CL-20 can be used to help understand and predict fate and impact (toxicity mechanisms) of the chemical and inform decisions about its potential future use in military munitions. (serdp-estcp.org)
The aim of this study was to evaluate new response metrics based on chemical and biological activity of NP for screening assays that can be used to predict NP toxicity in vivo. (cdc.gov)
Data from this study indicate that it is possible to predict acute in vivo inflammatory potential of NP with cell-free and cellular assays by using NP surface area-based dose and response metrics, but that a cellular component is required to achieve a higher degree of predictive power. (cdc.gov)

The variety of effects observed suggest the possible, and perhaps simultaneous, involvement of several toxicity mechanisms such as increased metabolic cost, reduced energy acquisition (potentially via inhibition of digestive enzyme activity), targeted inhibition of reproduction (potentially via inhibition of vitellogenesis), and/or direct inhibition of molting. (unboundmedicine.com)

Further research is needed to differentiate between these postulated mechanisms of dietary Cu toxicity and to determine whether they act separately or in concert. (unboundmedicine.com)
Significant advances have been made in the development of Adverse Outcome Pathways (AOPs) over the last decade, mainly focused on the toxicity mechanisms of chemicals. (cdc.gov)
In the long term, we strive to develop more realistic exposure scenarios in terms of environmental complexity and to identify specific properties and mechanisms related to toxicity. (lu.se)

Toxicokinetics and Cardiotoxicity is closely connected to Pharmacology in his research, which is encompassed under the umbrella topic of Toxicity. (research.com)

In parallel, several biological biochemical and biological assays were conducted to determine toxicity of the explosive towards various terrestrial, aquatic, and avian receptors. (serdp-estcp.org)
By reducing costs and time for protein engineering, and by working in a simple system that requires no knowledge of bioinformatics, cloning, cell culturing, and biochemical characterization, biologists and non-biologists alike will be able to conduct relevant biological engineering research and rapidly test protein design hypotheses. (sbir.gov)
Therefore, enough protein can be generated for detailed biochemical characterization and activity assays. (sbir.gov)

In the present study, the reproductive toxicity and histopathological effects of Monocrotophos pesticide on an exotic epigeic Eudrilus eugeniae and an indigenous epigeic Perionyx barotensis earthworm were studied. (gmsl.lk)
The present findings show that Monocrotophos pesticide on exposure to epigeic earthworm species causes significant reproductive toxicity and histopathological abnormalities and these changes could be used as a tool in environmental risk assessment of pesticides. (gmsl.lk)
Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)

Spirocyclic systems containing one common carbon atom to two rings are structurally interesting [ 10 ] and naturally occurring substances being known for their wide range of biological activities [ 11 , 12 ]. (ijpsonline.com)

Two cell-free and two cell-based assays were evaluated for their power in predicting in vivo toxicity of eight distinct particle types with widely differing physicochemical characteristics. (cdc.gov)

The data for Functional and other assays are more heterogeneous and therefore only columns containing the aggregated and filtered values for each specific type and unit of data, along with links to the corresponding assay descriptions in ChEMBL, are displayed. (optibrium.com)
For category B, a quite heterogeneous category, more toxicity data are needed to set an HNRV. (cdc.gov)

For many regulatory authorities, the local lymph node assay (LLNA) is the preferred assay for the predictive identification of skin-sensitizing chemicals. (cdc.gov)
Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)

Alterations of blood components are important biomonitoring tools in toxicological research because of the potentiality for rapid assessment of the chronic toxicities of a compound [ 41 ]. (eaht.org)
A trial on the quantitative risk assessment of man-made mineral fibers by the rat intraperitoneal administration assay using the JFM standard fibrous samples. (cdc.gov)

Here we present the results of an acute oral and a sub-chronic oral toxicity study in Beagle dogs. (nih.gov)

ACD/Labs offers a suite of tools that assess environmentally relevant qualities such as log D , aqueous solubility, aquatic toxicity, metabolomics, and more. (acdlabs.com)
There is a growing concern that dietborne metal toxicity might be important in aquatic ecosystems. (unboundmedicine.com)

Bioanalytical Assay Exposed: The Key To Unlocking Insights Into Biological Samples! (uscalifornia.com)
Bioanalytical assays are vital at all stages of the drug development process. (uscalifornia.com)
Hence, whether in vitro assay development or fully validated bioanalytical methods, unlocking the potential of bioanalytical assays is crucial in gaining insights into biological samples. (uscalifornia.com)
Newer bioanalytical assays are developed continuously to support drug candidates at various stages of development. (uscalifornia.com)
Hence robust bioanalytical assays will always remain crucial to maximize the generation of reliable and reproducible study data. (uscalifornia.com)
However, before employing a bioanalytical assay for routine purposes, assay qualification and validation become vital to demonstrate its suitability for clinical applications. (uscalifornia.com)
As a drug candidate reaches the clinical stages of drug development , bioanalytical assays will require full assay validation. (uscalifornia.com)
A completely validated bioanalytical assay is crucial as the drug compound is tested in human subjects. (uscalifornia.com)
Hence bioanalytical assays should be robust enough to accommodate minor modifications for incorporating newer alterations to the study. (uscalifornia.com)
The need for robust bioanalytical assays is well-appreciated in the scientific community. (uscalifornia.com)
Therefore, focusing on reliable bioanalytical assay development and validation is the key to delivering insightful data into biological samples. (uscalifornia.com)

The objective of this meta-analysis, which consisted of 36 studies containing 86 materials, was to assess the availability of published invivo rodent pulmonary toxicity data for a variety of nanoscale and microscale materials and to derive potency estimates via benchmark dose modeling. (cdc.gov)
Additionally, the potency estimates based on particle mass lung dose associated with acute pulmonary inflammation were used to group materials based on toxicity. (cdc.gov)

Although data concerning the potential toxicity of some NP have been reported, validated simple assays are lacking for predicting their in vivo toxicity. (cdc.gov)
The current standard for expressing panels of proteins involves extensive bioinformatics, cloning, in vivo expression, and assays. (sbir.gov)

This process should lead to improvements in the comparability and reproducibility of assay results within and between laboratories. (nist.gov)

The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
The study included six patients with stage III colorectal cancer with negative ctDNA and imaging results who had significant postoperative complications or were otherwise considered high risk for severe treatment toxicity. (medscape.com)

The composition of volatile oil of safflower dried flowers was analyzed by gas chromatography-mass spectrophotometry with flame ionization detector (GC-FID) and in vitro sensitivity assays were performed to assess biological activity. (techscience.com)

Their toxicity profiles differ when comparing canine safety data. (nih.gov)
In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
We constructed a genetically inactivated or attenuated strain containing relevant molecular assay targets and tested to compare assay performance using this strain to the historical data obtained using irradiation-inactivated virulent spores. (cdc.gov)
Following this, for Binding assays , aggregated and filtered pChEMBL values will be displayed for each data type for which an unqualified measurement has been reported (e.g. (optibrium.com)
IC 50 in μM), two columns are displayed containing aggregated and filtered values (see notes below) for that specific type and unit of data along with links to the corresponding assays descriptions in ChEMBL. (optibrium.com)
Leafy vegetables are popular in Benin but there is no scientific data available on their medicinal properties and toxicity. (biomedcentral.com)
This allows rapid access to biological data, and on-demand protein sequence prototyping. (sbir.gov)
1974. Biological handbooks: Biology data book. (cdc.gov)
These data will be used to estimate deficiencies and toxicities of specific nutrients in the population and subgroups, to provide population reference data, and to estimate the contribution of diet, supplements, and other factors to serum levels of nutrients. (cdc.gov)

Results indicated that NP are capable of producing reactive species, which in biological systems lead to oxidative stress. (cdc.gov)

To establish the extent of the biological relevance of these assays in predicting the effects in physiological systems, a high-level of confidence in the precision and robustness of these assay measurements is required. (nist.gov)

An array of tests used to determine the toxicity of a substance to living systems. (harvard.edu)
This graph shows the total number of publications written about "Toxicity Tests" by people in Harvard Catalyst Profiles by year, and whether "Toxicity Tests" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Toxicity Tests" by people in Profiles. (harvard.edu)

Such inactivated reference materials were used as positive controls in assays to detect suspected B. anthracis in samples because live agent cannot be shipped for use in field settings, in improvement of currently deployed detection methods or development of new methods, or for quality assurance and training activities. (cdc.gov)
Early detection of a biological threat is critical not only for identifying the threat organism but also for implementing appropriate countermeasures to save and protect the victims and prevent further infection and for decontaminating and reclamating the affected environment and infrastructures. (cdc.gov)
The bedrock of successful biodetection platforms and sensors is use of well-characterized molecular assays, immunoassays, or other types of detection assays. (cdc.gov)
[ 2 ] Although not completely reliable, an immunoassay and a mouse biologic assay are available for detection of ciguatoxin in affected fish. (medscape.com)

Digestibility and thermostability assays determined that Cry1Ia1 was fully digested within 30 s of exposure to pepsin and inactive after 3 to 4 minutes at 100 °C, indicating that it would not be a potential allergen. (ashs.org)
Supplement to documentation of the threshold limit values and biological exposure indices. (cdc.gov)
In this example, a biological exposure marker (blood lead-level) was available for classifying mothers according to their exposure to lead. (cdc.gov)

Western blot analysis and biological activity assays showed molecular and functional equivalency between 'SpuntaG2'-derived Cry1Ia1 protein and bacteria-derived Cry1Ia1 protein. (ashs.org)

To evaluate the therapeutic potential and toxicity effect of this leafy-vegetable, the antibacterial, antifungal, antioxidant activities and, toxicity and phytochemical constituents were investigated. (biomedcentral.com)

3) Hormone production of the adrenal cells was measured as toxicity endpoint. (nih.gov)
Differences in the biological effects of crocidolite asbestos and two glass fibres on epithelial lung cells. (cdc.gov)

The cell-free systems comprised fluorescence- and electron spin resonance-based assays of oxidant activity. (cdc.gov)
Copper NP had the greatest activity in all assays, while TiO2 and gold NP generally were the least reactive. (cdc.gov)
Differences in the ranking of NP activity among the assays were found when comparisons were based on measured responses. (cdc.gov)
Antioxidant activity was assessed using the 2,2-diphenyl-1-picryl-hydrazyl assay and phytochemical screening was carried out using standard procedures. (biomedcentral.com)
Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity. (bdbiosciences.com)

The volume of these containers can be reduced to fit into a cooler for transport, and the air space within kept to a minimum when portions are removed in the laboratory for the toxicity test or for chemical analyses. (canada.ca)
This could entail using cause-and-effect analysis to understand sources of variability in the assay, designing new plate layouts that include key process control measurements, and utilizing appropriate statistical analyses to understand the assay results and their uncertainties. (nist.gov)

Furthermore, it can be difficult to express complex proteins due to toxicity or purification difficulty, requiring labor-intensive diagnosis of expression and purification conditions. (sbir.gov)

His Toxicity research includes elements of Zebrafish and Pharmacology. (research.com)

We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)

Another source of ongoing research is how best to design in process control measurements such as the positive chemical control to maximize the confidence in the assay results. (nist.gov)
NICEATM and NC3Rs are partnering with the National Institute of Allergy and Infectious Diseases, the U.S. Army Combat Capabilities Development Command Chemical Biological Center, and NCATS to direct the MPS for COVID Research (MPSCoRe) working group. (nih.gov)
Concept of assessing nanoparticle hazards considering nanoparticle dosemetric and chemical/biological response metrics. (cdc.gov)

The toxicity of irgarol to estuarine gastropods has not been previously examined, although they have the potential to be exposed to antifoulants through both aqueous and sediment routes. (unboundmedicine.com)

This Technical Report provides basic background information on methods suitable to define the acute inhalation toxicity of fire effluents, as generated by fire models. (iso.org)
The methods developed as part of this platform also will allow greater access to biological engineering for K-12 and undergraduate students, requiring little capital or prior biological experience. (sbir.gov)
The primary reasons for the preferment of the LLNA are the animal welfare benefits it provides compared with traditional guinea-pig methods (refinement and reduction of animal usage) and the general performance characteristics of the assay with regard to overall reliability, accuracy, and interpretation. (cdc.gov)

If no prior PDL1 result is available at Screening, the subject must be assessed for PD-L1 result prior to the date of Randomisation using the stated method (PDL1 IHC 22C3 pharmDx assay) at a qualified local laboratory. (who.int)

Any assay development effort requires testing, evaluation, and validation of the assays with live or inactivated spiking materials in appropriate matrices relevant to the environments in which the assays are intended to be used (e.g., aerosol collection filters, soils, or clinical matrices). (cdc.gov)
95%) manufactured by Kalyani Industries Ltd., Mumbai, India was taken for evaluation of its toxicity. (eaht.org)

Hi everyone, I have a question about the error on certain biological results. (physicsforums.com)

Earthworms are an ideal biological model in toxicity assays and environmental monitoring studies. (gmsl.lk)
Researchers may not perform complete assay validation during early drug discovery studies. (uscalifornia.com)
However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)

Anatomy3

Organisms2

Diseases1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes15

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care2

Vitro assays4

Acute toxicity6

Developmental toxicity2

Systemic4

Compounds9

Cytotoxicity2

MeSH1

Antimicrobial1

Synthesis2

Computational3

DPPH2

Endpoints1

Concentrations2

Efficacy1

Predict3

Inhibition1

Mechanisms3

Cardiotoxicity1

Biochemical3

Reproductive3

Substances1

Physicochemical1

Heterogeneous2

Chemicals2

Assessment2

Chronic1

Aquatic2

Bioanalytical11

Pulmonary2

Vivo2

Reproducibility1

Severe2

Sensitivity1

Data10

Species1

Relevance1

Tests3

Detection4

Exposure3

Molecular1

Antifungal1

Cells2

Activity5

Analyses2

Diagnosis1

Pharmacology1

Proteins1

Chemical3

Potential1

Methods3

Laboratory1

Evaluation2

Results1

Studies3