• Mice also express an epidermal type 15-lipoxygenase (e-12LO) which has 50.8% amino acid sequence identity to human 15-LOX-2 and 49.3% sequence identity to mouse Arachidonate 8-lipoxygenase. (wikipedia.org)
  • The AA, a normal component of cell membrane phospholipids, serve as substrate for prostaglandin endoperoxide (PGH) syntases-1 and -2, also known as cycloxygenase (COX)-1 and -2, lipoxygenase (5-, 12-, or 15) (LO), or cytochrome p450 enzymes [ 18 ]. (hindawi.com)
  • during this metabolism, however, ALOX15 also forms 12(S)-HpETE as a minor product. (wikipedia.org)
  • Recent studies indicate that the metabolism by ALOXE3 of the R stereoisomer of 12-HpETE made by ALOX12B and therefore possibly the S stereoisomer of 12-HpETE made by ALOX12 or ALOX15 is responsible for forming various hepoxilins in the epidermis of human and mouse skin and tongue and possibly other tissues. (wikipedia.org)
  • Description: A competitive ELISA for quantitative measurement of Canine Arachidonate 15 lipoxygenase (ALOX15/LOG15) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (myelisakit.com)
  • Our objective in this study was to determine the extent to which tocotrienols inhibit platelet aggregation and reduce coronary thrombosis, a major risk factor for stroke in humans. (biomedcentral.com)
  • a mixture of α- + γ- + δ-tocotrienols) on in vivo platelet thrombosis and ex vivo platelet aggregation (PA) after intravenous injection in anesthetized dogs, by using a mechanically stenosed circumflex coronary artery model (Folts' cyclic flow model). (biomedcentral.com)
  • Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
  • Conversely, reactive oxygen species may favor the development of type 2 diabetes mellitus, mainly through the induction of insulin resistance. (biomedcentral.com)
  • For the quantitative determination of 15(S)-HETE in culture supernatants, plasma, serum, and urine from any species. (enzolifesciences.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Complement 1q (C1q) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (2sars.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Fucosidase Alpha L1, Tissue (FUCa1) in samples from serum, plasma, tissue homogenates or other biological fluids. (tuberculosistest.net)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Cyclin Dependent Kinase Inhibitor 3 (CDKN3) in samples from tissue homogenates or other biological fluids. (jemsec.com)
  • Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Brain Natriuretic Peptide (BNP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (2sars.com)
  • Description: Quantitativesandwich ELISA kit for measuring Human Complement 1q, C1q in samples from serum, plasma, tissue homogenates. (2sars.com)
  • Description: Quantitativesandwich ELISA kit for measuring Human Malate dehydrogenase, mitochondrial (MDH2) in samples from serum, plasma, tissue homogenates, cell lysates. (therabio.org)
  • Description: Quantitativesandwich ELISA kit for measuring Rat Hepatocyte nuclear factor 1-alpha (HNF1A) in samples from serum, plasma, cell culture supernates, tissue homogenates. (wildpalm.net)
  • Description: Quantitativesandwich ELISA kit for measuring Human A Disintegrin And Metalloprotease 9, ADAM9 in samples from serum, plasma, tissue homogenates, cell culture supernates, asciticfluid. (scalegen.com)
  • Description: Quantitativesandwich ELISA kit for measuring Rat brain natriuretic peptide, BNP in samples from serum, plasma, tissue homogenates. (2sars.com)
  • The present results indicate that intravenously administered tocotrienols inhibited acute platelet-mediated thrombus formation, and collagen and ADP-induced platelet aggregation. (biomedcentral.com)
  • The inhibitory effects of α-tocopherol on platelet function in vivo and ex vivo have been well established [ 14 - 18 ], but inhibition of platelet aggregation by α-tocopherol in vitro requires non-physiologically high levels of α-tocopherol [ 14 ]. (biomedcentral.com)
  • Collectively, these landmark discoveries provided initial insight into the COX pathway of arachidonate metabolism. (medscape.com)
  • The 15(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. (enzolifesciences.com)
  • It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) in tissue homogenates, cell lysates and other biological fluids. (resourcerecovery.be)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (resourcerecovery.be)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fucosidase Alpha L1, Tissue (FUCa1) in serum, plasma, tissue homogenates and other biological fluids. (tuberculosistest.net)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (therabio.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in Tissue homogenates, cell lysates and other biological fluids. (wildpalm.net)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (wildpalm.net)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Wilms Tumor Protein (WT1) in tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Wilms Tumor Protein (WT1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
  • Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Brain Natriuretic Peptide (BNP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (2sars.com)
  • Next, pharmacokinetic studies were carried out and tocol levels in canine plasma and platelets were measured. (biomedcentral.com)
  • Tocotrienols, given intravenously, could potentially prevent pathological platelet thrombus formation and thus provide a therapeutic benefit in conditions such as stroke and myocardial infarction. (biomedcentral.com)
  • Ferroptosis is a new type of programmed cell death, which occurs with iron dependence. (nature.com)
  • What is the relationship between ferroptosis and other types of cell death? (nature.com)
  • 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. (enzolifesciences.com)
  • Vascular endothelium is an active endocrine, paracrine, and autocrine organ, indispensable for the maintenance of vascular homeostasis [ 15 ]. (hindawi.com)
  • 12(R)-HpETE also spontaneously decomposes to a mixture of hepoxilins and trihydroxy-eicosatetraenoic acids that possess R or S hydroxy and epoxy residues at various sites while 8R-hydroxy-11R,12R-epoxy-hepoxilin A3 spontaneously decomposes to 8R,11R,12R-trihydroxy-5Z,9E,14Z-eicosatetraenoic acid. (wikipedia.org)
  • It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. (enzolifesciences.com)
  • CONCLUSIONS: Our findings demonstrate a shift in PMF types among US coal miners, from a predominance of coal- and mixed-type PMF to a more commonly encountered silica-type PMF. (cdc.gov)
  • The initial 12(S)-HpETE and 12(R)-HpETE products, regardless of their pathway of formation, are rapidly reduced to 12(S)-HETE and 12(R)-HETE, respectively, by ubiquitous cellular peroxidases, including in particular Glutathione peroxidases or, alternatively, are further metabolized as described below. (wikipedia.org)
  • Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. (kits-elisa.com)
  • In these roles, they may amplify or dampen, expand or contract cellular and tissue responses to disturbances. (wikipedia.org)
  • Brain tissue responses to ischemia. (southampton.ac.uk)
  • Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte type 12-lipoxygenase (also termed 12/15-lipoxygenase, 12/15-LOX or 12/15-LO) which is an ortholog of, and metabolically equivalent to, human 15-LO-1 in that it forms predominantly 15(S)-HpETE with 12(S)-HpETE as a minor product. (wikipedia.org)
  • More recent year of birth was significantly associated with silica-type PMF. (cdc.gov)
  • These results also indicate that intravenously administered tocotrienols were significantly better than tocopherols in inhibiting cyclic flow reductions (CFRs), a measure of the acute platelet-mediated thrombus formation. (biomedcentral.com)
  • In response to the presence of certain activating substances, including oxidized LDL, monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-8, and platelet-derived growth factor (PDGF), leukocytes migrate into the wall of the artery (see the image below). (medscape.com)
  • Microglia are efficient phagocytes of synaptic material and apoptotic cells, which are key processes in the developing brain 12 . (nature.com)
  • Although inflammation serves as a protective function in controlling infections and promoting tissue repair, it can also cause tissue damage and disease. (420magazine.com)
  • 12. Mitsios N, Gaffney J, Kumar P, Krupinski J, Kumar S, Slevin M. Pathophysiology of acute ischaemic stroke: an analysis of common signalling mechanisms and identification of new molecular targets. (southampton.ac.uk)
  • They are part of a much larger group of compounds, the eicosanoids, which, although ubiquitous as a group, are produced by a variety of cell type-, tissue-, and species-specific biosynthetic pathways. (jci.org)
  • COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
  • At 24hours after the exposure, significant increases in the pro-inflammatory markers IL-12p70, IL-13, IL-15, IFN-, TNF-, IL-17A, VEGF, MCP-1, and MIP-1 were noted in the basolateral cell culture medium of ABS-exposed cells compared to non-exposed chamber control cells. (cdc.gov)
  • Many cell types involved in this process express components of the cannabinoid signaling system that can be endogenously or pharmacologically controlled. (420magazine.com)
  • 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis. (enzolifesciences.com)
  • METHODS: We obtained lung tissue specimens and data from the National Coal Workers' Autopsy Study. (cdc.gov)
  • In 1930, American gynecologists Kurzok and Lieb first described the stimulatory effects of seminal fluid on human uterine muscle tissue. (medscape.com)
  • A few years later, von Euler of Sweden independently discovered similar effects of human seminal fluid on smooth muscle tissue. (medscape.com)
  • Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. (nature.com)
  • As expected, α-Tocopherol treatment increased levels of total tocopherols in post- vs pre-treatment specimens (57 vs 18 μg/mL in plasma, and 42 vs 10 μg/mL in platelets). (biomedcentral.com)
  • However, treatment with α-tocopherol resulted in slightly decreased levels of tocotrienols in post- vs pre-treatment samples (1.4 vs 2.9 μg/mL in plasma and 2.3 vs 2.8 μg/mL in platelets). (biomedcentral.com)
  • tocotrienols, 2.8 vs 0.9 μg/mL in plasma and 1.28 vs 1.02 μg/mL in platelets). (biomedcentral.com)
  • tocotrienols, 8.6 vs 1.7 μg/mL in plasma and 3.8 vs 3.9 μg/mL in platelets). (biomedcentral.com)
  • α-Tocotrienols treatment induced increases in α-tocopherol levels of 4-fold and 6-fold in plasma and platelets, respectively. (biomedcentral.com)
  • Interestingly, tocotrienols (TRF) treatment induced a less pronounced increase in the levels of tocotrienols in plasma and platelets, suggesting that intravenously administered tocotrienols may be converted to tocopherols. (biomedcentral.com)
  • RESULTS: Of 322 cases found to have PMF, study pathologists characterised 138 (43%) as coal-type, 129 (40%) as mixed-type and 55 (17%) as silica-type PMF. (cdc.gov)
  • Unlike COX-1, COX-2 expression is dramatically induced by ischemia and appears to be an effector of tissue damage. (southampton.ac.uk)
  • In contrast, the rate of silica-type PMF did not decline in cases from more recent birth cohorts. (cdc.gov)