AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
DNA MethylationMethylationDNA AdductsDNADNA, NeoplasmCpG IslandsEpigenesis, GeneticDNA (Cytosine-5-)-MethyltransferasePolymerase Chain ReactionSulfitesAzacitidinePromoter Regions, GeneticGene SilencingCytosineEpigenomicsDNA Modification Methylases5-MethylcytosineGenomic ImprintingHistonesLong Interspersed Nucleotide ElementsDNA-Cytosine MethylasesMethyltransferasesHistone-Lysine N-MethyltransferaseBase SequenceDinucleoside PhosphatesRNA, Long NoncodingSequence Analysis, DNAChromatinS-AdenosylmethionineGene Expression Regulation, NeoplasticGenome, HumanMolecular Sequence DataProtein MethyltransferasesDeoxyribonuclease HpaIICell Line, TumorLysineGene Expression RegulationGenetic LociS-AdenosylhomocysteineTranscription, GeneticAlu ElementsGenes, p16RNA, UntranslatedHeterochromatinOligonucleotide Array Sequence AnalysisAcetylationGene Expression ProfilingDNA, PlantArabidopsisGenes, Tumor SuppressorSite-Specific DNA-Methyltransferase (Adenine-Specific)Chromatin ImmunoprecipitationGenomeMutationCell LineHydroxamic AcidsReverse Transcriptase Polymerase Chain ReactionTumor Suppressor ProteinsDeath-Associated Protein KinasesInsulin-Like Growth Factor IIEmbryonic Stem CellsProtein-Arginine N-MethyltransferasesDNA-Binding ProteinsArabidopsis ProteinsRNA, MessengerCytidineRepressor ProteinsTranscription FactorsFolic AcidAllelessnRNP Core ProteinsChromatin Assembly and DisassemblyRNA, PlantCyclin-Dependent Kinase Inhibitor p16Polycomb-Group ProteinsGene Expression Regulation, DevelopmentalNuclear ProteinsTranscription Initiation SiteMethyl-CpG-Binding Protein 2NeoplasmsDNA PrimersGene Expression Regulation, PlantGenes, NeoplasmTumor Markers, BiologicalCyclin-Dependent Kinase Inhibitor p15EuchromatinModels, GeneticCluster AnalysisGlutathione S-Transferase piPhenotypeEmbryonic DevelopmentGene ExpressionColorectal NeoplasmsEpigenetic RepressionChromosomes, Human, XEmbryo, MammalianRepetitive Sequences, Nucleic AcidPregnancyJumonji Domain-Containing Histone DemethylasesHistone Deacetylase Inhibitors
GenesGenome wide methylationPatterns of DNA methylationEpigenome-wideHistoneMRNAEpigeneticsAdiposeCytosineFormalin-fixed paraffin-embeddedSpecific methylationDifferentialProteins450KSamplesBiomarkersEpithelialAssayIlluminaTranscriptionTumorsAlterationsEpigenetic regulationInflammationPeripheral tissuesTranscriptionalAnalysisMicroarrayExpressionFindingsResearchersGene regulationReprogrammingModificationWhole genomeWidespreadMediatesDNAmDifferentially methylatedGenomesBiomarkerSubcutaneousSignaturesCancerPlacental tissueOrganismsModificationsCancersMethyltransferasesPostmortemRegulatoryTranscriptomeMoleculeRegulateInvestigateMammalianInterestinglyDataFresh-frozenCells
Genes15
  • We tested a classification of the HMRs based on their distributions relative to transcription start sites (TSSs) and detected tissue-specific TSS-HMRs and genes that showed strong tissue effects. (umd.edu)
  • We aimed to determine whether DNA methylation and mRNA levels of the ABCA1 and ABCG1 genes in EAT and subcutaneous adipose tissue (SAT) were associated with CAD. (biomedcentral.com)
  • We established genome wide methylation profiles of various normal epithelial tissues and identified 110 genes that were differentially methylated in normal breast epithelium. (johnshopkins.edu)
  • A number of these genes also showed methylation alterations in breast cancers. (johnshopkins.edu)
  • Across the three phenotypes , we identified differentially methylated CpG sites and regions, differentially expressed genes (DEGs), and genes with concomitant differential methylation at their promoters and expression were identified. (bvsalud.org)
  • Concomitant study of DNA methylation and gene expression patterns may identify genes whose expression is epigenetically regulated in periodontitis . (bvsalud.org)
  • Transcription is the process by which genes carried by DNA are encoded in another form (RNA), this RNA is then translated into proteins. (pasteur.fr)
  • Changes in DNA methylation were especially prominent in genes that regulate the activity of neurons and the connectivity between them. (medscape.com)
  • Witt said it was also "fascinating that among those genes that showed the strongest changes in DNA methylation levels in our study, two genes were previously reported to regulate behavioral aspects of cocaine intake in rodent experiments. (medscape.com)
  • if the genes are not expressed in the right location, at the right time, or in the right amount due to genetic variations, the function of this tissue may be impaired and the risk of developing osteoarthritis may be increased. (medlineplus.gov)
  • The researchers investigated DNA methylation* at 480 000 points on the DNA and looked at how it affected the expression of the genes in the identical twins. (lu.se)
  • The cells of the body contain our genetic make-up (DNA), which contains genes. (lu.se)
  • DNA methylation is a chemical process that controls the function of the genes. (lu.se)
  • Methods: We analysed methylation status of 807 cancer-related genes in 189 fresh frozen primary breast tumours and four normal breast tissue samples using an array-based methylation assay. (lu.se)
  • Finally, by utilizing gene expression data, we observed that a large fraction of genes reported as having subtype-specific expression patterns might be regulated through methylation. (lu.se)
Genome wide methylation1
  • To this purpose, we herewith provide a relevant dataset of paired, tissue-specific, genome-wide methylation measurements in newborn tissues. (eur.nl)
Patterns of DNA methylation2
  • Three presentations from this year's Annual Meeting discussed a multicancer early detection test developed by GRAIL Inc., which utilizes machine learning and cell-free DNA from liquid biopsy samples to search for abnormal patterns of DNA methylation. (aacr.org)
  • Using patterns of DNA methylation as a measure of the biological age of cells, a total of 20 differentially methylated regions were significantly associated with CUD status after multiple testing correction. (medscape.com)
Epigenome-wide3
  • Therefore, we investigated the tissue specificity of epigenome-wide DNA methylation in newborn and placental tissues. (eur.nl)
  • DNA from 968 blood samples from children in the Study to Explore Early Development (SEED 1) was used to generate epigenome-wide array DNA methylation (DNAm) data at 485,512 CpG sites for 453 cases and 515 controls, using the Illumina 450K Beadchip. (springer.com)
  • Witt and colleagues assessed epigenome-wide DNA methylation signatures of CUD in human postmortem brain tissue of Brodmann area 9 (BA9) in 42 deceased males. (medscape.com)
Histone4
MRNA5
  • We further connected these findings to altered mRNA expression levels in adipose tissue (e.g. (lu.se)
  • Differential DNA methylation and mRNA transcription in gingival tissues in periodontal health and disease. (bvsalud.org)
  • We investigated differential DNA methylation in gingival tissues in periodontal health , gingivitis , and periodontitis , and its association with differential mRNA expression. (bvsalud.org)
  • Samples were processed for differential DNA methylation and mRNA expression using the IlluminaEPIC (850 K) and the IlluminaHiSeq2000 platforms, respectively. (bvsalud.org)
  • We have generated DNA methylation and mRNA expression array data from adipose tissue and skeletal muscle before and after a 6-months exercise intervention. (lu.se)
Epigenetics2
  • However, cancer is not an exclusively genetic disease and its progression is dependent on a host of additional biological processes such as immune activity, the tissue microenvironment, and epigenetics ( Hanahan and Weinberg, 2011 ). (frontiersin.org)
  • Epigenetics acts through two mechanisms: (1) modifications to chromosomal proteins that alter the 3D conformation of the genome and/or protein-DNA interactions and (2) chemical modification of the DNA strand itself ( Kondo, 2009 ). (frontiersin.org)
Adipose15
  • However, it is not well established whether human subcutaneous adipose tissue is affected by or affect circulating lipids through epigenetic mechanisms. (lu.se)
  • Hence, our aim was to investigate the association between circulating lipids and DNA methylation levels in human adipose tissue. (lu.se)
  • DNA methylation and gene expression were analysed genome-wide in subcutaneous adipose tissue from two different cohorts, including 85 men and 93 women, respectively. (lu.se)
  • Causal mediation analyses tested if adipose tissue DNA methylation mediates the effects of triglycerides on gene expression or insulin resistance. (lu.se)
  • We found 115 novel associations between triglycerides and adipose tissue DNA methylation, e.g. in the promoter of RFS1, ARID2 and HOXA5 in the male cohort (P ≤ 1.1 × 10-7), and 63 associations, e.g. within the gene body of PTPRN2 and COL6A3 in the female cohort. (lu.se)
  • Finally, a causal mediation analysis provided support for adipose tissue DNA methylation as a partial mediating factor between circulating triglycerides and insulin resistance. (lu.se)
  • This study identified novel epigenetic alterations in adipose tissue associated with circulating lipids. (lu.se)
  • Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the development of coronary artery disease (CAD). (biomedcentral.com)
  • Total body adiposity correlates with the amount of epicardial adipose tissue (EAT), a specific visceral fat accumulation around the myocardium in the proximity to the coronary arteries [ 2 ]. (biomedcentral.com)
  • We utilized the Multiple Tissue Human Expression Resource (MuTHER) and generated Illumina 450K adipose methylome data from 648 twins. (bcu.ac.uk)
  • Analysis of methylation quantitative-trait loci (metQTL) revealed that 28% of CpGs were associated with nearby SNPs, and when overlapping them with adipose expression quantitative-trait loci (eQTL) from the same individuals, we found that 6% of the loci played a role in regulating both gene expression and DNA methylation. (bcu.ac.uk)
  • Our results showed widespread population invariability yet sequence dependence on adipose DNA methylation but that incorporating maps of regulatory elements aid in linking CpG variation to gene regulation and disease risk in a tissue-dependent manner. (bcu.ac.uk)
  • They have made several groundbreaking discoveries such as genome-wide epigenetic modifications in pancreatic islets, skeletal muscle, adipose tissue and the liver from patients with type 2 diabetes compared with non-diabetic control subjects. (lu.se)
  • We investigate epigenomic and transcriptomic changes in skeletal muscle and adipose tissue and their association with obesity, insulin resistance, and metabolic risk. (lu.se)
  • The biopsies were taken from the vastus lateralis muscle and subcutaneous white adipose tissue. (lu.se)
Cytosine7
  • As of 2016, two nucleobases have been found on which natural, enzymatic DNA methylation takes place: adenine and cytosine. (wikipedia.org)
  • Cytosine methylation is widespread in both eukaryotes and prokaryotes, even though the rate of cytosine DNA methylation can differ greatly between species: 14% of cytosines are methylated in Arabidopsis thaliana, 4% to 8% in Physarum, 7.6% in Mus musculus, 2.3% in Escherichia coli, 0.03% in Drosophila, 0.006% in Dictyostelium and virtually none (0.0002 to 0.0003%) in Caenorhabditis or fungi such as Saccharomyces cerevisiae and S. pombe (but not N. crassa). (wikipedia.org)
  • The near-universal use of thymine exclusively in DNA and uracil exclusively in RNA may have evolved as an error-control mechanism, to facilitate the removal of uracils generated by the spontaneous deamination of cytosine. (wikipedia.org)
  • The best characterized chemical modification of DNA is the methylation of cytosine to 5-methylcytosine (5mC) that occurs almost exclusively in the context of a cytosine base linked by the DNA phosphate-backbone to guanosine, termed a CpG site. (frontiersin.org)
  • Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
  • Moreover, the CpG site methylation is mediated by DNA methyltransferases (DNMTs), which catalyze the addition of a methyl group to cytosine ( 13 , 14 ). (spandidos-publications.com)
  • DNMT1 preserves the methyltransferase by binding to hemi-methylated CpG sites and methylates the cytosine on the newly synthesized strand after DNA replication, whereas DNMT3a/DNMT3b are required for the de novo genomic methylation of DNA ( 15 ). (spandidos-publications.com)
Formalin-fixed paraffin-embedded1
  • MethCORR modelling of methylomes from formalin-fixed paraffin-embedded tissue enables characterization and prognostication of colorectal cancer. (ega-archive.org)
Specific methylation2
  • Here we studied breast specific methylation in purified normal epithelium and its reflection in breast cancers. (johnshopkins.edu)
  • Conclusions: We have found that breast cancers of the basal-like, luminal A and luminal B molecular subtypes harbour specific methylation profiles. (lu.se)
Differential2
Proteins2
  • Researchers have explored diverse strategies for detecting cancer from liquid biopsy samples, including analyzing circulating tumor cells (CTCs), proteins, and/or cell-free DNA shed from tumors. (aacr.org)
  • DNA meth-ylation represses transcription by interfering with transcription factor binding and indirectly by recruiting methyl-CpG-binding proteins and reducing chromatin remodeling activities ( 12 ). (spandidos-publications.com)
450K2
Samples17
  • ABCA1 and ABCG1 DNA methylation analysis was performed in 24 samples from the CAD group and 9 samples from the NCAD group via pyrosequencing. (biomedcentral.com)
  • DNA methylation levels at the ABCG1 cg27243685 site positively correlated with plasma triglyceride concentration (r = 0.510, p = 0.008), body mass index (r = 0.556, p = 0.013) and waist-to-hip ratio (r = 0.504, p = 0.012) in SAT samples. (biomedcentral.com)
  • (C) Methylation values from 450 K analysis of 36 DNA samples extracted from breast epithelial cells from expressed breast milk. (biomedcentral.com)
  • (D) Validation of the methylation levels using pyrosequencing in a separate set of 53 EBM samples (black solid line). (biomedcentral.com)
  • The pattern of methylation is very similar to that of the 450 K analysis of EBM samples and of ER-pos cancer cell lines (dashed line) with high levels of variability at p2 for both. (biomedcentral.com)
  • MethCORR identifies genome-wide correlations between RNA expression and DNA methylation in fresh-frozen samples. (ega-archive.org)
  • This information is used to infer gene expression information in FFPE samples from their DNA methylation profiles. (ega-archive.org)
  • MethCORR was applied to methylation profiles from 877 fresh-frozen and FFPE samples and comparative analysis identified the same two major subtypes in four independent cohorts. (ega-archive.org)
  • Missing persons projects require specialized technology for both the recovery of DNA from compromised samples and the analysis of degraded DNA. (qiagen.com)
  • Overcome the challenges of limited and degraded DNA samples in missing persons cases with the QIAGEN bone processing workflow. (qiagen.com)
  • Learn how our full Sample to Insight workflow for DNA profiling with Pyrosequencing delivers greater knowledge from forensic samples to inform investigations and create new leads. (qiagen.com)
  • EZ1 has dedicated protocols for the purification of DNA from bulk samples of ground bones and teeth. (qiagen.com)
  • Plus, we offer pre- and post-PCR cleanup kits to maximize the potential of a sample, as well as specialized DNA profiling kits, such as the Investigator 24plex QS Kit and DIPplex Kit, for degraded DNA samples. (qiagen.com)
  • Using DNA methylation data from previous publications with data collected in house for a total 1189 samples spanning 193,651 CpG sites, we developed 4 novel epigenetic clocks by choosing different regression models (elastic net- versus ridge regression) and by considering different sets of CpGs (all CpGs vs highly conserved CpGs). (sens.org)
  • The expression of the identified long noncoding RNAs was downregulated in cancer tissue samples compared with normal tissue samples, and these long noncoding RNAs correlated with a favorable prognosis in breast cancer patients and the patients in the pancancer cohort. (cancerindex.org)
  • We will analyze DNA methylation in prostate tissue samples from WTCHP patients and from a group of age and stage matched prostate cancers not related to WTC. (cdc.gov)
  • The researchers explored genome-wide DNA methylation profiles in samples of endemic Burkitt lymphoma tissue and identified a molecular signature shared by both Epstein-Barr virus infection and aflatoxin B1 exposure that affects the expression of a putative tumour suppressor, TGFBI. (who.int)
Biomarkers2
  • This allows DNA methylation cancer biomarkers to be suitable for early detection and also to have utility across a range of areas relevant to cancer detection and treatment. (frontiersin.org)
  • This review will summarize existing DNA methylation cancer biomarkers used in clinical practice across the application domains above, discuss what makes a suitable DNA methylation cancer biomarker, and identify barriers to translation. (frontiersin.org)
Epithelial4
  • We elaborated on one of them, TRIM29 (ATDC), and showed that its promoter was hypo-methylated in normal breast epithelium and heavily methylated in other normal epithelial tissues. (johnshopkins.edu)
  • Intragenic DNA methylation in ESR1 in exfoliated breast epithelial cells from expressed breast milk (EBM). (biomedcentral.com)
  • Characterization of accessible chromatin regions in cattle rumen epithelial tissue during weaning. (usda.gov)
  • Finally, the nasal lesions associated with exposure to high concentrations of MeI in rats are best supported by a mode of action that involves glutathione (GSH) depletion in the nasal epithelial tissue. (cdc.gov)
Assay3
  • To help forensic labs overcome these challenges, we've developed dedicated products and protocols, including robust instrumentation for sample preparation and a complete analytical assay portfolio, that are optimized for compromised DNA. (qiagen.com)
  • Moreover, in the ketamine group, the level of Ten‑Eleven‑Translocation methylcytosine dioxygenase for demethylation as determined by reverse transcription‑quantitative PCR assay was increased in comparison with the control group, but that was not the case for the level of DNA methyltransferases for methylation. (spandidos-publications.com)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
Illumina2
  • The findings were validated using our earlier databases using HG-U133Plus2.0Affymetrix microarrays and Illumina (450 K) methylation arrays. (bvsalud.org)
  • Genome-wide DNA methylation was measured by the Illumina HumanMethylation450K BeadChip. (eur.nl)
Transcription2
Tumors1
Alterations2
  • In this issue, we generally review the mechanisms of cellular senescence in diabetic nephropathy, which involve telomere attrition, DNA damage, epigenetic alterations, mitochondrial dysfunction, loss of Klotho, Wnt/ β -catenin signaling activation, persistent inflammation, and accumulation of uremic toxins. (hindawi.com)
  • As DNA methylation is an important regulatory mechanism for gene expression, the identified DNA methylation alterations might contribute to functional changes in the human brain and thereby to the associated behavioral aspects of addiction," first author Eric Poisel, a PhD student at the Central Institute of Mental Health in Mannheim, said in the release. (medscape.com)
Epigenetic regulation3
  • Overall, these findings emphasize the linkage between breast specific epigenetic regulation and tissue specificity of cancer. (johnshopkins.edu)
  • For this reason, the team of Shahragim Tajbakhsh from Institut Pasteur, in collaboration with the team of Wolf Reik at the Babraham (UK) tried to decipher the relationships between epigenetic regulation of DNA and ageing muscle stem cells. (pasteur.fr)
  • The mechanism of epigenetic regulation involves the CpG site methylation of promoter regions and the modification of DNA and histones by altering chromatin structure ( 9 - 11 ). (spandidos-publications.com)
Inflammation2
  • The immune system, which plays a role in healing injuries, targets these areas, and its response leads to inflammation of the joint tissues. (medlineplus.gov)
  • In addition, the cartilage damage triggers an immune response, causing inflammation of other joint tissues and leading to further joint damage. (medlineplus.gov)
Peripheral tissues2
  • Thus, we need to rely on peripheral tissues for our studies. (stanford.edu)
  • Genome-wide DNA methylation comparison between live human brain and peripheral tissues within individuals - paper cited over 50 times since published in 2019. (stanford.edu)
Transcriptional4
  • Transcriptional modifications in nociceptive pathways are pivotal for the development and the maintenance of pain associated with tissue damage. (researchgate.net)
  • This study also confirmed that age-related DNA methylation is associated to a certain extent with transcriptional modifications. (pasteur.fr)
  • The present study investigated the methylation of CpG sites in the cyclooxygenase (COX)‑2 promoter via nuclear factor (NF)‑κB transcriptional regulation and elucidated its effect on the COX‑2 transcriptional expression in a ketamine‑induced ulcerative cystitis (KIC) animal model. (spandidos-publications.com)
  • Moreover, DNA hypomethylation of the COX‑2 promoter region located from ‑1,522 to ‑829 bp might contribute to transcriptional regulation of COX‑2 expression and induce a pro‑inflammatory response in KIC. (spandidos-publications.com)
Analysis4
Microarray1
Expression9
  • Moreover, in breast carcinomas methylation increased and expression decreased whereas the reverse was noted for multiple other carcinomas. (johnshopkins.edu)
  • Spearman correlations between methylation and expression in the EPIC/mRNAseq dataset were largely replicated in the 450 K/Affymetrix datasets. (bvsalud.org)
  • Methylation reprogramming was correlated with increased gene expression variability. (jneurosci.org)
  • DNA methylation is a modification on DNA that can guide gene expression, and multiple studies have suggested that abnormal DNA methylation may be indicative of cancer. (aacr.org)
  • The results of the present study revealed that ketamine treatment induced NF‑κB p65 translocation to nuclei and activated COX‑2 expression and prostaglandin (PGE)2 production in bladder tissue, whereas COX‑2 inhibitor suppressed the inflammatory effect. (spandidos-publications.com)
  • Multi-walled carbon nanotube s elicit concordant changes in DNA methylation and gene expression following long-term pulmonary exposure in mice. (cdc.gov)
  • To elucidate whether epigenetic effects play a role for these gene expression changes, we performed whole genome bisulphite sequencing to assess DNA methylation patterns in the lungs 56 days after exposure to MWCNTs. (cdc.gov)
  • Making a family decision to donate the brain for genomic research: lessons from the genotype-tissue expression project (GTEx). (cdc.gov)
  • GWAS as well as expression microarrays and global DNA-methylation measurements on muscle, fat and blood have been conducted. (lu.se)
Findings1
  • Recent findings suggest that DNA methylation changes are related to the development and progression of a group of human diseases including atherosclerosis. (biomedcentral.com)
Researchers4
  • Seeking understand the decline in function of tissues in organisms, researchers from the Stem Cells and Development unit at Institut Pasteur made a discovery on the still unknown mechanisms of the epigenetic clock in stem cells. (pasteur.fr)
  • In recent years, researchers have examined the utility of cell-free DNA methylation patterns for early cancer detection. (aacr.org)
  • Anthropology researchers are faced with the challenge of purifying DNA from bones and teeth and creating profiles of potentially degraded DNA. (qiagen.com)
  • The question the researchers posed was whether epigenetic* changes in the DNA lead to changes in the fat tissue that in turn can lead to the development of type 2 diabetes. (lu.se)
Gene regulation2
  • Epigenetic modifications such as DNA methylation play a key role in gene regulation and disease susceptibility. (bcu.ac.uk)
  • The epigenome consists of chemical modifications attached to our DNA, which can be altered due to environmental factors, e.g., exercise, and thereby provides dynamic gene regulation and gives each cell or tissue its unique identity and functions. (lu.se)
Reprogramming2
Modification1
  • We also show that SpCas9-mediated cleavage is unaffected by DNA methylation and that the dosage of SpCas9 and sgRNA can be titrated to minimize off-target modification. (cdc.gov)
Whole genome1
  • Using Holstein cattle, we generated 29 whole genome bisulfite sequencing (WGBS) datasets for 16 tissues, 47 corresponding RNA-seq datasets, and 2 whole genome sequencing datasets. (umd.edu)
Widespread1
Mediates1
  • In contrast, the Ten-Eleven-Translocation (TET) dioxygenase family, including TET1, TET2 and TET3, mediates active DNA demethylation and hydroxylate-methylated DNA by converting 5-methylcyto-sine to 5-hydroxymethylcytosine to regulate DNA methylation status. (spandidos-publications.com)
DNAm1
  • We, in collaboration with Iowa neurosurgeon Dr. Hiroto Kawasaki , are collecting brain tissues resected for the treatment of refractory epilepsy, together with saliva and blood taken before and after surgery, so that we can test DNAm correlation among them at specific loci. (stanford.edu)
Differentially methylated1
  • Using UC-WBC as a reference tissue, we identified 3595 HUVEC tissue-specific differentially methylated regions (tDMRs) and 11,938 placental tDMRs. (eur.nl)
Genomes1
  • High CpG methylation in mammalian genomes has an evolutionary cost because it increases the frequency of spontaneous mutations. (wikipedia.org)
Biomarker2
  • These properties make cancer-associated DNA methylation changes very attractive for development of cancer biomarker tests with substantive clinical utility. (frontiersin.org)
  • The ability of circulating tumor DNA liquid biopsy assays to monitor cancer in situ also allows for the ability to monitor response to therapy, to detect minimal residual disease and as an early biomarker for cancer recurrence. (frontiersin.org)
Subcutaneous1
  • Evaluation of tissue response to dental alloys by subcutaneous implantation. (cdc.gov)
Signatures1
  • This study reveals significant association between blood-based decreased S100P methylation and BC, and provides another proof for the application of altered DNA methylation signatures from blood cells as potential markers for the detection of BC, especially for the early stage. (uni-koeln.de)
Cancer4
  • Cancer is a heterogeneous and tissue-specific disease. (johnshopkins.edu)
  • It is suggested that tissue differentiation, mediated mostly by epigenetic modifications, could guide tissue-specific susceptibility and protective mechanisms against cancer. (johnshopkins.edu)
  • Furthermore, DNA methylation patterns are relatively consistent between different types of cancer, making this method more widely applicable. (aacr.org)
  • To compare DNA methylation as a marker of tumor aggressiveness in WTCHP prostate cancer patients and in matched prostate cancers not related to WTC. (cdc.gov)
Placental tissue2
  • Umbilical cord white blood cells (UC-WBC, n = 25), umbilical cord blood mononuclear cells (UC-MNC, n = 10), human umbilical vein endothelial cells (HUVEC, n = 25) and placental tissue (n = 25) were obtained from 36 uncomplicated pregnancies. (eur.nl)
  • We hypothesized that maternal gestational glucose levels would positively correlate with DNA methylation of the PPARGC1A promoter in placental tissue. (portlandpress.com)
Organisms2
  • In plants and other organisms, DNA methylation is found in three different sequence contexts: CG (or CpG), CHG or CHH (where H correspond to A, T or C). In mammals however, DNA methylation is almost exclusively found in CpG dinucleotides, with the cytosines on both strands being usually methylated. (wikipedia.org)
  • The DNA methylation landscape of vertebrates is very particular compared to other organisms. (wikipedia.org)
Modifications1
  • Epigenetic modifications have been proposed as a major cause of age-related tissue degradation. (pasteur.fr)
Cancers2
  • Searching for abnormal methylation patterns in cell-free DNA could, therefore, allow for early detection of a wide range of cancers using a noninvasive blood sample. (aacr.org)
  • Our results suggest that methylation may play an important role in the development of breast cancers. (lu.se)
Methyltransferases1
Postmortem1
  • In a novel study of postmortem brain tissue, "we detected a trend towards stronger biological aging of the brain in individuals with cocaine use disorder compared to individuals without cocaine use disorder," lead investigator Stephanie Witt, PhD, Central Institute of Mental Health, Mannheim, Germany, said in a news release. (medscape.com)
Regulatory2
  • Although DNA methylation has been well studied in humans and other model species, its distribution patterns and regulatory impacts in cattle are still largely unknown. (umd.edu)
  • A multi-tissue atlas of regulatory variants in cattle. (usda.gov)
Transcriptome1
  • By examining the transcriptome and epigenome of individual muscle stem cells simultaneously, we found that DNA transcribes* more chaotically in older muscle stem cells compared to those in younger mice. (pasteur.fr)
Molecule1
Regulate1
  • We know that fat tissue can release hormones and regulate metabolism in different organs in the body. (lu.se)
Investigate2
  • Collectively, these models provide a range of experimental approaches to investigate the molecular and cellular mechanisms directing the morphogenesis of the craniofacial tissues and associated organs in health and disease. (grc.org)
  • We aimed to investigate whether the molecular subtypes also display distinct methylation profiles. (lu.se)
Mammalian2
  • In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. (nih.gov)
  • 3699 Adenine methylation has been observed in bacterial, plant, and recently in mammalian DNA, but has received considerably less attention. (wikipedia.org)
Interestingly1
  • Interestingly, there was no overlap between methylation sites associated with triglycerides in men and the sites found in women, which points towards sex-specific effects of triglycerides on the epigenome. (lu.se)
Data3
  • Here, we present the largest collection of cattle DNA methylation epigenomic data to date. (umd.edu)
  • We further used these WGBS data to detect 50,023 experimentally supported CGIs across bovine tissues and found that they might function as a guard against C-to-T mutations for TSS-HMRs. (umd.edu)
  • It should be noted that epigenetic clocks based on DNA methylation are constructed from data from multiple tissues from a mixture of different cell types. (pasteur.fr)
Fresh-frozen1
  • genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions. (nih.gov)
Cells7
  • Non-CpG methylation can however be observed in embryonic stem cells, and has also been indicated in neural development. (wikipedia.org)
  • In mammals, around 75% of CpG dinucleotides are methylated in somatic cells, and DNA methylation appears as a default state that has to be specifically excluded from defined locations. (wikipedia.org)
  • In epigenetic association studies, DNA methylation is often examined in DNA derived from white blood cells, although its validity to other tissues of interest remains questionable. (eur.nl)
  • While older stem cells show a slight increase in the methylation of their DNA, this methylation does not correspond to their chronological age. (pasteur.fr)
  • The observed BC-associated decreased S100P methylation in blood mainly originates from the leucocytes subpopulations but not B cells. (uni-koeln.de)
  • However, differences in blood methylation patterns may not reflect differences in brain cells or liver cells. (cdc.gov)
  • Clinical studies in bladder tissue of KIC patients have shown increases in the infiltration of eosinophil and mast cells as well as serum immunoglobulin (Ig)E level, which displayed an association with hypersensitivity and/or allergic reactions ( 7 , 8 ). (spandidos-publications.com)