• Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
  • In vivo experiments confirmed mitochondrial abundance and mitochondrial oxygen consumption rates were elevated in host tissues following MSC treatment. (frontiersin.org)
  • Prior to their clinical application, hASCs must be expanded ex vivo to obtain the required number of cells for transplantation. (biomedcentral.com)
  • Karyotype analysis was performed and tumorigenesis assay in vivo was also evaluated. (biomedcentral.com)
  • Moreover, the hASCs presented normal karyotype undergoing senescence, and did not form in vivo tumors, eliminating the possibility that spontaneous immortalization of hASCs had occurred with pooled allogeneic human serum. (biomedcentral.com)
  • The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
  • In addition, you will be part of the studies determining the in vivo efficacy and safety of new compounds using preclinical tumor models and the development of a companion biomarker. (academictransfer.com)
  • We are looking for a post-doc researcher with a proven track record in Molecular Biology or Biochemistry and an interest in drug development, stem cell biology, and in vivo models. (academictransfer.com)
  • One was ex vivo and one in vivo and both illustrated the inhibition characteristics vitamin C has on new tumor blood vessel growth. (prnewswire.com)
  • The in vivo assay treated with vitamin C indicated 30% less blood vessel growth than untreated tissue. (prnewswire.com)
  • The level of insulin growth factor-1 receptor expression is directly correlated with the tumor uptake of (111)In-IGF-1(E3R) in vivo and the clonogenic survival of breast cancer cells exposed in vitro to trastuzumab (Herceptin). (ox.ac.uk)
  • We address these questions in vivo with unprecedented cell specificity and comprehensiveness, utilizing innovative methods to investigate how genome structure and organization influences gene expression specifically in the C. elegans germ line. (yale.edu)
  • In vitro and in vivo testing of purified BTIC subsets were performed to verify multipotency, self-renewal, and capacity to generate glioblastoma tumors in murine cranial xenotransplantation models. (cns.org)
  • Both BTIC subtypes were validated in vitro and in vivo as demonstrating multi-potency, self-renewal, and capacity to generate glioblastoma tumors in murine xenotransplants. (cns.org)
  • However, it was not until the 1980s when researchers began looking for ways to grow mouse sarcoma cells and develop genetically manipulable mouse models that they became fully capable of examining the direct causes of many cancers in an in vivo setting . (3dprint.com)
  • in vivo angiogenesis studies and augmentation of tumors in immunosuppressed mice. (3dprint.com)
  • In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
  • In summary, BCL9-2 induces ER positive breast cancers in vivo , regulates ER expression by a novel ß-catenin independent mechanism in breast cancer cells, and might predict the therapy response to tamoxifen treatment. (oncotarget.com)
  • Of course, in the body, that's not how it behaves at all-tumors are in 3D masses…and a drug that looks good on plastic doesn't necessarily mean that it will be good in vivo. (genengnews.com)
  • To demonstrate cell behaviors in vivo, pancreatitis mouse model with overexpressing ST6GAL1 was created, and immunofluorescent staining was done on pancreas tissue samples. (uab.edu)
  • Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
  • In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)
  • Upon its transition to the precancerous Barrett's esophagus epithelium, however, a small dedicated stem cell compartment appears that supports proliferation of the epithelium while concomitantly evidence for a stochastically dividing compartment contributing to the maintenance of the tissue disappears. (wikipedia.org)
  • Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
  • This study investigated the effect of EZH2 expression on proliferation and tumorigenesis of brain glioma cells. (medscimonit.com)
  • Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
  • Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)
  • Cell proliferation assay CCK\8 (Dojindo, Japan) assay was utilized to judge cell proliferation based on the manufacturer's guidelines. (biotech2012.org)
  • This complete characterization of hASCs cultivated in pooled allogeneic human serum, a suitable xeno-free approach, shows that pooled allogeneic human serum provides a high proliferation rate, which can be attributed for the first time to C-MYC protein expression, and showed cell stability for safe clinical applications in compliance with good manufacturing practice. (biomedcentral.com)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • In this position , you will be part of a team (ERC-PoC, NWO Demonstrator) to characterize several lead compounds using cell-based assays measuring the potency and specificity of Notch/g-secretase inhibitors and the effects of Notch activity, proliferation, differentiation, and self-renewal of normal and tumor tissues. (academictransfer.com)
  • This groundbreaking BCRI study has shown that angiogenesis or the proliferation of new blood vessels, in support of tumor growth, is retarded when high levels of vitamin C are present in the blood. (prnewswire.com)
  • Gran1 was selectively internalized by macrophages, the major host cell of Mtb , and restricted the proliferation of the pathogen. (uni-ulm.de)
  • Interference with and overproduction of GRP78 in vitro impacted the proliferation, migration, invasion, and metastasis of HCC cells. (bvsalud.org)
  • Their larval and adult cells have narrow potencies, sometimes coupled to virtually unlimited … proliferation, and function in the growth, maintenance and regulation of body size. (iospress.com)
  • The embryos of larger arthropods and deuterostomes with well-provisioned eggs or viviparity, on the other hand, exhibit regulative development, while their larval "set-aside" or adult stem cells function in the growth, maintenance, and regulation of organ size coupled to constrained proliferation and cell turnover. (iospress.com)
  • Mammalian embryonic stem cells would seem adapted to rapid proliferation, functioning in part to enclose yolk or to acquire access to maternal resources. (iospress.com)
  • Mammalian adult stem cells resemble the blastomeres of planktonic and benthic organisms with small eggs and may have evolved in mature organisms as an adaptation to the growth and maintenance of tissues via proliferation and the regulation of organ size via cell loss (e.g., terminal differentiation). (iospress.com)
  • Moreover, Corning's Matrigel matrix offers a network of proteins that provide the environment needed for optimal tissue performance, driving cellular responses such as proliferation and attachment. (3dprint.com)
  • Moreover, BCL9-2 regulated the expression of ER and the proliferation of human breast cancer cells independently of ß-catenin. (oncotarget.com)
  • This study utilizes proliferation assays, clonogenic assays, death assays and soft agar colony formation assays using murine pancreatic acinar cancer cells and immortalized normal human pancreatic acinar cells to demonstrate ST6GAL1 influencing stem-like cell behaviors in vitro. (uab.edu)
  • Overexpression of ST6GAL1 resulted in not only, increased proliferation and colony formation but iv also, decreased cell death when treated with IFN-γ and TNF-α. (uab.edu)
  • However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
  • The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). (cdc.gov)
  • Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
  • Загальні відомості про плазмоклітинні захворювання Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)
  • Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
  • CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
  • Such cells are hypothesized to persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumors. (wikipedia.org)
  • In addition, it is further related with occurrence and metastasis of malignant tumors. (medscimonit.com)
  • growth, invasion, metastasis and enhanced the manifestation of EMT and POSTN in tumor cells. (biotech2012.org)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • Among others are the breast cancer stem cells (BCSCs), which are believed to have a contribution to tumorigenesis, metastasis, and resistance against chemotherapy. (hindawi.com)
  • In this study, we sought to investigate the mechanism underlying the inhibitory effect of PPI on the invasion and metastasis of SR-HCC cells. (bvsalud.org)
  • The results indicated that PPI administration decreased the levels of proteins associated with metastasis and markers of drug resistance in tumor tissues, impeding tumor growth and spread. (bvsalud.org)
  • Cancer stem cells (CSCs) are considered to be the main reason for tumor relapse and metastasis, CSCs can maintain the number or generate more CSCs by self-renewal, and generate non-CSCs progeny by differentiation. (biomedcentral.com)
  • Approaches to characterize tumor development, progression, and metastasis in these models using state-of-the-art imaging, histopathological, surgical, and other techniques are also included. (cshlpress.com)
  • Cancer stem cells, instrumental in metastasis, would seem to ignore mechanisms normally functioning in the removal of excess cells. (iospress.com)
  • A section of a rare lymph node metastasis from adenocarcinoid tumor (250 X). Image courtesy of Professor Pantaleo Bufo, University of Foggia, Italy. (medscape.com)
  • Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
  • This model suggests that only certain subpopulations of cancer stem cells have the ability to drive the progression of cancer, meaning that there are specific (intrinsic) characteristics that can be identified and then targeted to destroy a tumor long-term without the need to battle the whole tumor. (wikipedia.org)
  • BACKGROUND: Breast cancer stem cells (BCSCs) have a crucial role in breast carcinogenesis, development, and progression. (harvard.edu)
  • A workshop was convened by the AACR to discuss the rapidly emerging cancer stem cell model for tumor development and progression. (aacrjournals.org)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • We investigated the presence of aluminium (Al) in human colon cancer samples and its potential association with biological processes involved in cancer progression, such as epithelial to mesenchymal transition (EMT) and cell death. (bvsalud.org)
  • Currently a couple of no exams that measure phenotypic or genotypic markers on tumor cells that anticipate progression (8). (oaxacalibre.net)
  • Hematological and neurological expressed 1 (HN1) is upregulated in many tumors, but the role of HN1 in breast cancer progression and its regulatory mechanism have not been well understood. (biomedcentral.com)
  • To study the role of HN1 in the initiation and progression of breast cancer, we examined HN1 levels in breast cancer cells and tissues and analyzed the relationship between HN1 levels and patient survival. (biomedcentral.com)
  • Combined with miRNAs-based transcriptome sequencing assay, it was found that Tanshinone IIA downregulated miR-125b level and upregulated its target gene STARD13 (StAR-related lipid transfer protein 13) level, thus inactivating the miR-125b/STARD13 axis, which had been previously confirmed to promote breast cancer progression. (biomedcentral.com)
  • This study successfully uncovers cell behaviors that promote the transition from ADM to neoplasia, offering novel avenues to inhibit PDAC progression. (uab.edu)
  • The somatostatin analogs (SSAs) octreotide and lanreotide are used to control carcinoid symptoms and tumor progression in advanced inoperable disease. (medscape.com)
  • Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
  • Within the lab, four PI-led teams focus on identifying actionable dependencies in tumor cells and the tumor microenvironment that can be targeted to enhance and prolong treatment response while preserving normal tissue function. (academictransfer.com)
  • The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)
  • Our team focusses on understanding the cellular and molecular crosstalk between keratinocytes- the epithelial cells of the skin- and their microenvironment in health and disease. (ugent.be)
  • Our team has developed a new biopsy technology that can profile multiple tumor microenvironment biomarkers simultaneously, revealing cellular spatial organization and interactions that will help advance personalized disease diagnosis and treatment. (aacu.org)
  • The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
  • The Notch signaling pathway is an evolutionary conserved signal transduction cascade present in almost all tissues and is required for embryonic and postnatal development, as well as for stem cell maintenance, but it is also implicated in tumorigenesis including pancreatic cancer and leukemia. (uni-ulm.de)
  • Such a high dose of growth factors results in a range of complications, including uncontrolled tissue formation, inflammation and tumorigenesis, the production or formation of tumor cells. (sciencedaily.com)
  • We used mammosphere formation assay, side population analysis, wound healing assay, transwell assay, soft agar formation assay, and xenografted tumor model to determine the effect of HN1 on the expansion of breast cancer stem cells, and the migration, invasion and tumorigenesis of breast cancer. (biomedcentral.com)
  • In breast cancer cell line, ectopic overexpression of HN1 not only promoted the expansion of breast cancer stem cells, but also promoted cell migration, invasion, and tumorigenesis, while knockdown of HN1 reduced these effects. (biomedcentral.com)
  • Overexpression of HN1 not only promoted the self-renewal of breast cancer stem cells, but also promoted migration, invasion, and tumorigenesis. (biomedcentral.com)
  • METHODS: Fresh tumor tissue samples were obtained from 31 breast cancer (BC) patients for (1) Mammosphere culture. (harvard.edu)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Bringing a compound through the entire drug discovery and development value chain is associated with a lot of risk due to high costs, long lead times and the use of many different methods and assays. (ddw-online.com)
  • This has led to the development of 3D cell culture technologies and methods that use aggregates of cells, often human primary or differentiated stem cells into tissue or organ-like structures associated with the indications of a disease. (ddw-online.com)
  • Together with Gen5 Image Prime image analysis software, the platform provides a comprehensive tool for quantitative assays particular to 3D cell culture methods. (ddw-online.com)
  • METHODS: The tumor and normal tissue uptake of [(111)In]-IGF-1 and [(111)In]-IGF-1(E3R) were determined at 4 h postinjection in mice implanted subcutaneously with MDA-MB-231, H2N, HR2 or MCF-7/HER2-18 human BC xenografts (8.5x10(4), 1.4x10(4), 4.0x10(4) and 1.0x10(5) IGF-1R/cell, respectively). (ox.ac.uk)
  • The new test, they say, is cheaper and faster than other methods currently available, and it requires only small tissue samples. (medicalnewstoday.com)
  • MATERIALS AND METHODS: A systemic search of gene expression profiles of multiple tissues in NSCLC was carried out to determine the expression intensities of traditional fibroblast biomarkers and CAF-secreted protumorigenic cytokines. (cdc.gov)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • Methods of ctDNA analysis include single-locus assays, targeted sequencing approaches, and whole genome sequencing. (medscape.com)
  • Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
  • In particular, in mice, whose life spans do not exceed two years, tumor relapse is difficult to study. (wikipedia.org)
  • we've done it for mice with liver diseases where we treat the mice with liver stem cells or liver mini-organs, that's one thing, so regenerative medicine. (ecancer.org)
  • Histological examination of tumor tissue substantiated that administering PPI via gavage to HepG2/S xenograft nude mice inhibited tumor growth and significantly reduced tumor size, as evidenced by xenograft experiments involving nude mice. (bvsalud.org)
  • IGFBP-3), and the level of IGF-1 receptor (IGF-1R) expression on human breast cancer (BC) xenografts in athymic mice, as well as the feasibility for tumor imaging. (ox.ac.uk)
  • MicroSPECT/CT imaging was performed on mice with MCF-7/HER2-18 tumors injected with [(111)In]-IGF-1(E3R). (ox.ac.uk)
  • Sperm DNA methylation is predominantly stable in mice offspring bornafter transplantation of long-term cultured spermatogonial stem cells. (diagenode.com)
  • Specifically, the trainee looked at the ability of an endothelial cell AGO 1 gene knockout to rescue streptozotocin-induced damage in female mice. (ca.gov)
  • Moreover, aged BCL9-2 transgenic mice developed ductal-like mammary tumors with high nuclear ER expression. (oncotarget.com)
  • Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
  • Besides customer-tailored cell line development as a one-stop shop, Evercyte is steadily expanding its catalog of readily available cell lines for the production of extracellular vesicles from mesenchymal stem cells using different tissues including Wharton's Jelly, adipose tissue, bone marrow, and placenta. (biospace.com)
  • By providing native extracellular vesicles from mesenchymal stem cells and targeting extracellular vesicles Evercyte will make a significant contribution to the development of new therapeutic approaches. (biospace.com)
  • CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
  • At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
  • CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
  • Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. (wikipedia.org)
  • CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells. (wikipedia.org)
  • CSCs may generate tumors through the stem cell processes of self-renewal and differentiation into multiple cell types. (wikipedia.org)
  • As CSCs form a small proportion of the tumor, this may not necessarily select for drugs that act specifically on the stem cells. (wikipedia.org)
  • Hence, at least for certain neoplastic tissues, dedicated stem cell compartments maintain and enlarge the size of the transformed compartment The cancer stem cell model, also known as the Hierarchical Model proposes that tumors are hierarchically organized (CSCs lying at the apex (Fig. 3). (wikipedia.org)
  • Essentially this theory proposes that all cells have the ability to be tumorigenic making all tumor cells equipotent with the ability to self-renew or differentiate, leading to tumor heterogeneity while others can differentiate into non-CSCs The cell's potential can be influenced by unpredicted genetic or epigenetic factors, resulting in phenotypically diverse cells in both the tumorigenic and non-tumorigenic cells that compose the tumor. (wikipedia.org)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • The meeting participants were charged with evaluating data suggesting that cancers develop from a small subset of cells with self-renewal properties analogous to organ stem cells. (aacrjournals.org)
  • Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
  • BCSC is a subset of tumor cells that has stem cell-like characteristics, such as a high capacity for self-renewal and tumor initiation, which implies that BCSC may cause aggressiveness of TNBC. (hindawi.com)
  • Breast cancer stem cell is a subset of tumor cells that has a characteristic of stem cells, having a high capacity of self-renewal and tumor initiations, which imply that BCSC may cause aggressiveness of TNBC [ 5 ]. (hindawi.com)
  • A second trainee examined the role of Sae2 in DNA replication, a critical step in growth and self-renewal of pluripotent stem cells. (ca.gov)
  • Transwell migration assay CP-466722 To evaluate the effect of BMMSC on migration of malignancy cells, Transwell assay was also used. (biotech2012.org)
  • 104 cells in 300?L serum\free DMEM were placed in CP-466722 each Transwell chamber (Corning Inc., Corning, NY, USA), and 700?L regular MSC\CM or control medium were placed in the lower chamber. (biotech2012.org)
  • Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
  • The concept of a non-contact force being able to direct a cell's motility is potentially interesting for brain cancer therapy when you consider that controlling tumor dispersion-or possibly undoing it-could be a major boon to the management of brain tumors. (nature.com)
  • Our first trainee focused on the impact of pseudouridine in coding and noncoding RNAs as an effector of glioblastoma stem cells (GSC) and the development of glioblastoma multiforme brain tumors. (ca.gov)
  • We found, that primary cell cultures of BCL9-2 breast tumors responded to tamoxifen treatment. (oncotarget.com)
  • In contrast, transcriptional regulation of the ESR1 gene promoter was described in a number of studies to be an important mechanism for overexpression and maintenance of ER expression in breast tumors [ 1 ]. (oncotarget.com)
  • Imaging studies also must be performed to detect the sites of either primary tumors or metastases. (medscape.com)
  • Mouse models of malignant tumors called sarcomas have provided a fundamental tool for researchers to understand the pathology behind human cancers. (3dprint.com)
  • Within the cancer population of the tumors there are cancer stem cells (CSC) that are tumorigenic cells and are biologically distinct from other subpopulations They have two defining features: their long-term ability to self-renew and their capacity to differentiate into progeny that is non-tumorigenic but still contributes to the growth of the tumor. (wikipedia.org)
  • In tumor angiogenesis, the blood vessels grow to support the growth of the tumor. (prnewswire.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • The use of hASCs for therapeutic applications has grown substantially in the last years, because the use of stem cells from adult tissues circumvent some ethical issues associated with the application of embryonic stem cells, and because of their accessibility via isolation from lipoaspirates, a disposable byproduct of cosmetic surgery. (biomedcentral.com)
  • Profoundly different patterns of potency and division are exhibited by mammalian embryonic and adult stem cells. (iospress.com)
  • The cellular products of embryonic stem cells routinely come under global influences and give rise to the cells of germ layers and organ rudiments. (iospress.com)
  • Strategies for regenerative therapies in adult mammals, therefore, might be based on stimulating growth of adult stem cells or their surrogates in specific tissues rather than on introducing embryonic stem cells into adults. (iospress.com)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • 3) Flow cytometry (FCM) assay using CD44, CD24, and EpCAM. (harvard.edu)
  • 4) RT-PCR profiler Arrays using stem cell (SC) panel of 84 genes for four group of cells (1) CD44 + /CD24 - /EpCAM - BCSCs, (2) CD44 + /CD24 - /EpCAM + BCSCs, (3) mammospheres, and (4) normal breast tissues. (harvard.edu)
  • Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • In the study published in the February 2010 issue of Journal of Angiogenesis Researc h, two assays were used to evaluate the effect of high-doses of vitamin C on the inhibition of new blood vessel growth. (prnewswire.com)
  • In different tumor subtypes, cells within the tumor population exhibit functional heterogeneity and tumors are formed from cells with various proliferative and differentiation capacities. (wikipedia.org)
  • They analyzed the whole-genome bisulfite sequencing profiles of 45 esophageal tumors, representing both the squamous cell carcinoma and adenocarcinoma subtypes, and matched nonmalignant tissue. (genomeweb.com)
  • In this study, single-cell microfluidic genetic profiling of primary human glioblastoma was performed to characterize intra-tumoral BTIC heterogeneity, identify unique surface markers of BTIC subsets, and associate BTIC subsets with clinically-relevant bulk tissue molecular subtypes. (cns.org)
  • Each BTIC subtype was characterized by distinct surface markers, and single-cell molecular profiles relating to distinct bulk tissue molecular subtypes. (cns.org)
  • This is the first demonstration of cancer stem cell heterogeneity in glioblastoma at the single-cell level, and particularly as it relates to prospective isolation and bulk tumor subtypes. (cns.org)
  • Persons with the most common pediatric brain malignancy, medulloblastoma, fare only slightly better than average, with a 71.9% 5-year survival, though the most common adult brain tumor, glioblastoma multiforme (GBM), incurs a mere 5.1% 5-year survival rate 2 . (nature.com)
  • Human adipose tissue-derived stem cells (hASCs) are attractive cells for therapeutic applications and are currently being evaluated in multiple clinical trials. (biomedcentral.com)
  • Maastro lab, led by Marc Vooijs, is developing more effective and safer treatments for cancer patients by targeting the Notch stem cell pathway: a high-priority clinical target. (academictransfer.com)
  • There are various types of tumors in breast cancers, which also possess different characteristics, clinical outcomes, and therapeutic responses. (hindawi.com)
  • This tumor subtype tended to show a worse clinical course, with earlier and more frequent recurrence and worse 5-year survival, compared with non-TNBC [ 4 ]. (hindawi.com)
  • The finding that both BTIC subsets were present in reproducible proportions across patients, and from both de novo and recurrent tumors, suggest these subpopulations are not differentially affected by current clinical interventions. (cns.org)
  • Termed the Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA), the fluorescence imaging-based technology can spatially profile a large number of mRNA and protein markers in cells and tissues, including clinical tumor tissues. (aacu.org)
  • [ 5 ] The researchers characterized genomic architecture and inferred the clonal evolution using eight tumor biopsies and nine plasma samples collected over 1193 days of clinical follow-up using exome and targeted amplicon sequencing. (medscape.com)
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
  • Understanding the activity of this gene will allow for an improved understanding of how cells can survive DNA damage leading to genomic rearrangements that support the initial stages of tumorgenesis and subsequent cancer development. (ca.gov)
  • Through the lens of genomic and functional assays. (lu.se)
  • ctDNA was successfully detected in 97% women in whom somatic genomic alterations were identified, and CA 15-3 and circulating tumor cells were detected in 78% and 87%, respectively. (medscape.com)
  • Fluorescence in-situ hybridization (FISH) assays on fresh (including Amniotic fluid, chorionic villi sampling, peripheral blood, product of conception, tumour tissue) and FFPE tissues. (kkh.com.sg)
  • This functional heterogeneity among cancer cells has led to the creation of multiple propagation models to account for heterogeneity and differences in tumor-regenerative capacity: the cancer stem cell (CSC) and stochastic model. (wikipedia.org)
  • Using molecular modeling, biochemical and functional assays, as well as single-molecule time-lapse imaging, we show that RBPJL and RBPJ, despite limited sequence homology, possess a high degree of structural similarity. (uni-ulm.de)
  • The Singh Laboratory, managed by Singh, leverages high-throughput functional assays and perturbations to dissect the functional regulatory programs in mammalian cells. (sciencedaily.com)
  • We wish to understand how germ cells protect their DNA, while turning different genes on and off at the right times to make functional sperm and eggs. (yale.edu)
  • Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
  • Wound repair can however predispose tissues to cancer initiation and we aim to identify novel molecular mechanisms through which inflammation, cell death and fibrosis modulate epidermal stem cells and impact on skin regeneration and neoplasia. (ugent.be)
  • 4. Can we manipulate molecular signaling pathways in the skin to improve tissue regeneration, while preventing tumour initiation? (ugent.be)
  • Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation. (ugent.be)
  • This hypothesis predominantly focuses on the transition between normal pancreatic acinar to tumor cell and the importance of ST6GAL1 in the tumor initiation process on which few studies have been reported. (uab.edu)
  • The lab led by Associate Professor Sofie Mohlin focuses on the physiology of healthy embryogenesis and tumor initiation. (lu.se)
  • A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
  • This engineering research group is led by Dr. Akhilesh Gaharwar, associate professor of biomedical engineering and Presidential Impact Fellow, in collaboration with Dr. Irtisha Singh, assistant professor in the Department of Molecular and Cellular Medicine at Texas A&M and the co-corresponding author of the study where a new class of mineral-based nanoparticles has been introduced to direct human stem cells toward bone cells. (sciencedaily.com)
  • One of the major findings of this study is that minerals such as silicon, magnesium and lithium are involved in inducing endochondral ossification, a process by which stem cells are transformed into soft and hard tissues such as cartilage and bone in young humans. (sciencedaily.com)
  • harm that can consist of lytic bone tissue lesions, anemia, immunodeficiency, and reduced renal function (2). (oaxacalibre.net)
  • The phenotype from the MM stem/tumor-propagating cell Unlike Compact disc138C or Compact disc38C cells, Compact disc138+ or Compact disc38+ MM cells can proliferate and induce lytic bone tissue lesions when transplanted into ectopic bone tissue in SCID-hu or SCID-rab immunodeficient mouse versions (19). (oaxacalibre.net)
  • Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)
  • Diagnosis typically requires demonstration of M-protein (sometimes present in urine and not serum but rarely absent entirely) and/or light-chain proteinuria, and excessive plasma cells in the bone marrow. (msdmanuals.com)
  • [ 1 ] All bones are encased in a soft tissue envelope known as the periosteum, which is vital for perfusion and nutrient supply to the outer third of the bone (see the image below). (medscape.com)
  • Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
  • In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
  • 90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)
  • Existing cancer treatments have mostly been developed based on animal models, where therapies able to promote tumor shrinkage were deemed effective. (wikipedia.org)
  • The efficacy of cancer treatments is, in the initial stages of testing, often measured by the ablation fraction of tumor mass (fractional kill). (wikipedia.org)
  • Cancer stem cells were first identified by John Dick in acute myeloid leukemia in the late 1990s. (wikipedia.org)
  • This cell model suggests these mutations could occur to any cell in the body resulting in a cancer. (wikipedia.org)
  • According to the "stochastic model" (or "clonal evolution model") every cancer cell in a tumor could gain the ability to self-renew and differentiate to the numerous and heterogeneous lineages of cancer cells that compromise a tumor These mutations could progressively accumulate and enhance the resistance and fitness of cells that allow them to outcompete other tumor cells, better known as the somatic evolution model. (wikipedia.org)
  • Meanwhile, at the DMR level, the researchers found more than 10,000 hypo-DMRs between the two esophageal cancer types and also note that many of the methylation changes there were also present in normal cells. (genomeweb.com)
  • Cancer Cell Int. 2022 Dec 31;22(1):423. (harvard.edu)
  • Prof Clevers talks to e cancertv at WIN 2014 about using single stem cells to grow tissue for cancer research and potentially cancer treatment. (ecancer.org)
  • By combining those two observations, Wnt drives cancer but Wnt drives healthy stem cells as well, we came up with a novel marker, LGR5, which is now widely used as a stem cell marker and LGR5 helped us to find the colon stem cell but also stem cells of many other tissues. (ecancer.org)
  • Now, in cancer it is the epithelium that is actually the malignant tissue and a tumeroid would be an organoid not grown from a normal stem cell but grown from a cell that comes out of a tumour. (ecancer.org)
  • Also when they are normal tissue people would have predicted that they would probably have to become cancer cells to grow for long times outside the body, that's not what we find. (ecancer.org)
  • Mutations that activate oncogenes and deactivate tumor suppressor genes are widely recognized as significant contributors to cancer development. (bvsalud.org)
  • Plasma concentrations of arsenic (As), cadmium (Cd), chromium (Cr), mercury (Hg), and lead (Pb) were measured using inductively coupled plasma mass spectrometry (ICPMS), and next-generation sequencing (NGS) of 1123 cancer-related genes was performed using the tumor tissues. (bvsalud.org)
  • If the cancer stem cell model is correct and if such cells retain the hallmarks of some tissue stem cells in being rare and entering the cell cycle infrequently, they could constitute a population that is intrinsically resistant to current therapies designed to kill cycling cells. (aacrjournals.org)
  • In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)
  • These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
  • If cancer stem cells are relatively refractory to therapies that have been developed to eradicate the rapidly dividing cells within the tumor that constitute the majority of the nonstem cell component of tumors, then they are unlikely to be curative and relapses would be expected. (aacrjournals.org)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
  • ALDH1 has a role in early stem cell differentiation through its function in the oxidation of retinol to retinoic acid, proposed to be a strong candidate for breast cancer stem cells. (hindawi.com)
  • A total of 54 of 55 patients treated at RSCM were tested for the expression of ALDH1 through an immunohistochemistry assay of breast cancer tissue using ALDH1 staining. (hindawi.com)
  • Transmission electron microscopy and energy-dispersive X-ray microanalysis confirmed the presence of Al specifically in intracytoplasmic electrondense nanodeposits adjacent to mitochondria of colon cancer cells. (bvsalud.org)
  • Al-positive samples showed a significant increase in both the number of vimentin-positive and nuclear ß-catenin-positive cancer cells compared to Al-negative samples. (bvsalud.org)
  • Identification of cell surface markers of distinct BTIC subpopulations will support future study of glioblastoma cancer stem cells, and the potential development of BTIC subpopulation-specific therapeutic strategies. (cns.org)
  • Improved understanding of the underlying cancer biology of this subpopulation of cancer stem cells, thought to drive tumor formation and therapeutic resistance, could potentially lead to improved targeted therapies that prolong patient survival. (cns.org)
  • BACKGROUND: Immune evasion in glioblastoma (GBM) shields cancer cells from cytotoxic immune response. (diagenode.com)
  • To determine whether HN1 regulates MYC, we used quantitative real-time PCR and Western blot analysis to assess the expression of MYC and their targeted genes to determine the phenotype caused by knockdown of MYC in breast cancer cell with HN1 overexpression. (biomedcentral.com)
  • In this study, we found that HN1 was upregulated in breast cancer tissues. (biomedcentral.com)
  • Downregulation of MYC abrogated the effect of HN1 overexpression in breast cancer cell lines. (biomedcentral.com)
  • Here, we found HN1 to be upregulated in breast cancer tissues, and patients with high HN1 expression had poor prognosis. (biomedcentral.com)
  • In this study we investigate the properties of populations of brain cancer undergoing electrotaxis, a phenomenon whereby cells are directed to migrate under control of an electrical field. (nature.com)
  • Likewise, strategies for the containment of cancer might be based on promoting normal pathways of cell loss, the basal mode for handling excess cells. (iospress.com)
  • From the development of several types of tumor cell invasion assays to exploring organoid model environments, from cancer and stem cell research to neurobiology, tissue engineers, biologists, and industry giants are using Matrigel matrix as a foundational biomaterial for diverse applications. (3dprint.com)
  • Now, it can also be used on the Allevi Platform to 3D bioprint cancer spheroids with a variety of cell lines. (3dprint.com)
  • City of Hope's Train, Educate and Accelerate Mastery of Stem cell research (TEAMS) program was built around four key areas of regenerative medicine research: Cancer Stem Cells, Hematopoietic Stem Cells, Tissue-Specific Stem Cells and Human Pluripotent Stem Cells. (ca.gov)
  • Within the field of Cancer Stem Cells we had four trainees. (ca.gov)
  • One trainee focused on developing in vitro growth conditions to optimize expansion of luminal epithelial cells and c-KIT+ progenitor cells to explore the early stages of breast cancer. (ca.gov)
  • Two students focused on establishing transgenic mouse models to explore the impact of androgen receptor, hepatocyte growth factor and human c-MET on the development of prostate cancer tumors. (ca.gov)
  • Within the field of Hematopoietic Stem Cells one trainee explored the impact of SGF29, a protein that recognizes methylated histones and modulates transcription, in leukemic cancer stem cells. (ca.gov)
  • The ultimate goal is to use ATOs to develop cancer fighting T cells for immunotherapy applications. (ca.gov)
  • Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. (biomedcentral.com)
  • Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
  • The study has previously revealed that miR-125b faciliates cell migratory ability breast cancer via targeting STARD13 (StAR-related lipid transfer protein 13) [ 20 ]. (biomedcentral.com)
  • I served as a member of Breast Cancer Campaign Scientific Advisory Board and I am a member of the Breast Cancer Now Tissue Access Committee. (nottingham.ac.uk)
  • T cells play a critical role in the control of cancer. (aacrjournals.org)
  • The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
  • Dr. Weian Zhao is a tenured full Professor and entrepreneur at the Sue and Bill Gross Stem Cell Research Center, Chao Family Comprehensive Cancer Center, Department of Biomedical Engineering, and Department of Pharmaceutical Sciences at University of California, Irvine. (aacu.org)
  • With this holistic view of a variety of cancer cell types it will enable scientists and researchers to personalize diagnosis and treatment. (aacu.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • People with prostate cancer are at risk of metastatic tumors forming. (medicalnewstoday.com)
  • The new test detects copy number alterations (CNAs), which are changes in the genome that drive the spread of cancer tumors. (medicalnewstoday.com)
  • By assessing CNAs in samples of blood or prostate tissue, specialists can get a better idea of whether or not the cancer cells are proliferating. (medicalnewstoday.com)
  • We believe the addition of the NG-CNA assay onto a standard cancer gene testing platform will augment personalized medicine by identifying aggressive tumors and genetic mutations that are predictors of response to targeted therapies," Dr. Ostrer declares. (medicalnewstoday.com)
  • Moreover, given that this enzyme is upregulated in cancer and our preliminary data shows that ST6GAL1 drives the cell towards a more Acinar to Ductal Metaplasia-like phenotype, I hypothesize that expression of ST6GAL1 promotes stem-like cell behaviors, such as enhanced survival and invasiveness, thereby promoting neoplasia. (uab.edu)
  • In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
  • Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
  • Chemicals causing mammary gland tumors in animals signal new directions for epidemiology, chemicals testing, and risk assessment for breast cancer prevention. (who.int)
  • In the past 5 years, there has been an explosion of breast cancer research on circulating tumor DNA (ctDNA), fueled by the idea that ctDNA may provide a liquid biopsy alternative to tissue biopsies. (medscape.com)
  • Speaking about the promise of liquid biopsies in breast cancer, Sarah-Jane Dawson, the PhD group leader of the Cancer Therapeutics Program at Peter MacCallum Cancer Centre, Victoria, Australia, said she didn't think that ctDNA would replace tissue biopsies, but tissue biopsies clearly have limitations that include being invasive and difficult to perform serially. (medscape.com)
  • Cancer cells release small fragments of ctDNA into the bloodstream, most commonly through apoptosis. (medscape.com)
  • In a recent study, researchers compared radiographic imaging of tumors with an assay of ctDNA, cancer antigen 15-3 ( CA 15-3 ), and circulating tumor cells in 30 women with MBC who were receiving systemic therapy. (medscape.com)
  • Cell apoptosis assay CAL 27 or HN4 cells were cultured in charge or MSC\CM media at 37C for 48?hours and apoptotic cells were detected using FITC\Annexin V LFNG antibody and propidium iodide (BD Biosciences). (biotech2012.org)
  • Autophagy critically controls skin inflammation and apoptosis-induced stem cell activation. (ugent.be)
  • RBPJL is specifically expressed in the exocrine pancreas, whereas it is mostly undetectable in pancreatic tumour cell lines. (uni-ulm.de)
  • PDAC can arise when pancreatic acinar cells undergo acinar-to-ductal metaplasia (ADM), giving rise to premalignant pancreatic intraepithelial neoplasias (PanINs), which finally progress to PDAC. (uni-saarland.de)
  • Multi-omic analyses revealed a higher tumor mutational burden (TMB) in Al-positive colon cancers (n = 2) compared to a control cohort (n = 100). (bvsalud.org)
  • Tumor relapse is the main cause of the high mortality of various cancers. (biomedcentral.com)
  • These dismal survival rates are due in part to a lack of available therapies for cancers in neural tissues, as the brain is a particularly difficult organ to treat. (nature.com)
  • Even with continual advances in drug discovery and delivery technologies that have substantially improved outcomes in systemic cancers, there has been disappointingly little impact on tumors of the brain. (nature.com)
  • Thus, there is an urgent need to innovate new strategies to safely manage or treat brain cancers and therein a substantial need for a better understanding of how brain tumor dissemination can be limited or controlled. (nature.com)
  • Overall, the results showed that overexpression of ST6GAL1 provides the cells with a greater proliferative, pro-survival and invasive phenotype. (uab.edu)
  • Nanosilicates dissociate into individual minerals inside the cells and turn "on" a set of key genes that result in information flow throughout the cells, known as signaling pathways. (sciencedaily.com)
  • These signaling pathways are responsible for instructing the cells to take on specific functions, such as converting into another type of cells or starting the healing process by secreting tissue-specific proteins known as extracellular matrix. (sciencedaily.com)
  • RNA-seq, a transcriptome-wide high throughput sequencing assay, provides an unbiased and holistic overview of the gene expression profiles to identify pathways that are perturbed by specific treatments. (sciencedaily.com)
  • We find that U87 mg electrotaxis is abolished under pharmacological inhibition of PI3Kγ, mTOR, AKT and ErbB2 signaling, whereas DAOY cell electrotaxis was not attenuated by these or other pathways evaluated. (nature.com)
  • 2. Which molecular pathways control the activation of epidermal stem cells? (ugent.be)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • A collaboration between researchers in the Department of Biomedical Engineering and the College of Medicine at Texas A&M University identifies the crucial role of minerals in regulating gene expression, thus controlling the number of proteins that a cell should make, thereby encouraging tissue regeneration and redefining cellular identity. (sciencedaily.com)
  • Here, we look to electrical fields and their influence on cellular behavior as a means to control tumor dispersion, using the regime of low field-strength direct current, wherein electrotaxis occurs. (nature.com)
  • Together with mapping of disease-related genes, this transcriptomic mapping of the developing human spinal cord opens new avenues for interrogating the cellular basis of motor control in humans and guides human stem cell-based models of disease. (uni-saarland.de)
  • These modifications led a reduction of cellular growth as compared to a control population of untreated cells. (ca.gov)
  • In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
  • What about the survival of organoids and tumeroids grown from single stem cells? (ecancer.org)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • The majority of the biologic markers that are proven to have an association with TNBC resistance against therapy are found in the cytoplasm of TNBC cells. (hindawi.com)
  • Surface markers of distinct BTIC subpopulations were validated in fluorescence-activated cell sorting (FACS) and colony forming assays. (cns.org)
  • To our knowledge, this is the first demonstration of the prominent cell-type specificity of PMDs across normal, precursor, and malignant states," the researchers write. (genomeweb.com)
  • however, tumors can originate from any cell of the amine precursor uptake and decarboxylation system and, therefore, produce several intestinal hormones. (medscape.com)
  • Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)
  • Hematoxylin and eosin (HE) staining of tumor tissue specimens, along with immunohistochemistry (IHC), were conducted to evaluate the expression levels of Ki67, GRP78, N-cadherin, Vimentin, and ABCG2. (bvsalud.org)
  • Cell viability and differentiation capacity toward the mesenchymal lineages were assessed, along with immunophenotype. (biomedcentral.com)
  • Cultures are subjected to a standard 2D viability assay to determine the health of the cells two days after treatment with the test compound. (genengnews.com)
  • Regeneration is mediated by the molecular processes that regulate gene expression to control tissue renewal, restoration and growth. (sciencedaily.com)
  • These nanoparticles are known specifically as nanosilicates, and with them, the team is able to determine the role of minerals in regulating gene expression profiles to direct stem cell differentiation. (sciencedaily.com)
  • In this study, they analyzed whole transcriptomic sequencing (RNA-seq) data to evaluate the effect of nanosilicates and ionic dissolution products on the gene expression profiles of stem cells. (sciencedaily.com)
  • How does genome organization stabilize and instruct tissue-specific gene expression? (yale.edu)
  • This analysis led to the discovery of a population of preadipocyte cells whose gene expression is remodeled in an age-specific fashion. (ca.gov)
  • Fetal bovine serum is the supplement most widely used for cell culture, but it has disadvantages and it is not safe for cell therapy due to the risks of pathogen transmission and immune reaction. (biomedcentral.com)
  • DNA methylation changes from primary cultures through senescence-bypassin Syrian hamster fetal cells initially exposed to benzo[a]pyrene. (diagenode.com)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • A second trainee examined the relationship between aging and the activation of adipocyte progenitor cells. (ca.gov)
  • Early fate decisions in hematopoietic stem and progenitor cells. (lu.se)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • We see no mutations, we see no structural changes to the chromosomes so it really looks like this is an indefinite culture of normal or malignant cells. (ecancer.org)
  • These cells guard their DNA very carefully to allow the production of sperm and eggs with the right number of chromosomes and no mutations. (yale.edu)
  • In this project the candidate will use chick embryos, patient tissue material, and omics data to investigate how hotspot mutations in susceptibility genes affect healthy embryogenesis, organ formation and initation of the endocrine tumor form paraganglioma. (lu.se)
  • The stem mutations, mutations that are carried in the bulk of an individual's disease, are going to be best for telling you something about tumor burden. (medscape.com)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • Monoclonal gammopathy of undetermined significance is certainly a common premalignant tumor that precedes MM Monoclonal gammopathy of undetermined significance (MGUS) includes a prevalence of 4% in Caucasians older than 50 (6, 7). (oaxacalibre.net)
  • Rarely, patients have no M-protein in blood and urine, although the currently used serum free light chain assay now demonstrates monoclonal light chains in many of these formerly so-called nonsecretory patients. (msdmanuals.com)
  • The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
  • Microfluidic genetic profiling of BTICs at the single-cell level was performed to characterize intra-tumoral BTIC heterogeneity. (cns.org)
  • The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
  • Using a sequence-aware multi-model PMD caller they developed, dubbed MMSeekR, the researchers uncovered a high level of heterogeneity at the methylation level and in the distribution of PMDs across tumor samples. (genomeweb.com)
  • These data support BTIC subpopulation heterogeneity as an underlying source of intra-tumoral bulk tissue molecular heterogeneity. (cns.org)
  • Here we transcriptomically profiled the midgestation human spinal cord with single-cell resolution and discovered remarkable heterogeneity across and within cell types. (uni-saarland.de)
  • Andersen, Thom and colleagues reveal the single-cell-resolution transcriptome of the midgestation human spinal cord and discover remarkable heterogeneity across and within cell types. (uni-saarland.de)
  • MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
  • Either mechanism is different from the widely held notion that most cells in a tumor should be competent for tumor formation. (aacrjournals.org)
  • WICHITA, Kan. , Feb. 26 /PRNewswire/ -- Bio-Communications Research Institute's (BCRI's) recent research study has found that high levels of vitamin C (ascorbate) inhibit the formation of new blood vessel growth to tumors. (prnewswire.com)
  • Possibly the regular cell this is the focus on of change provides this unusual immunophenotype, but no-one provides identified a standard BM or principal lymphoid tissues cell with this phenotype. (oaxacalibre.net)
  • This shows that the tumor-propagating cell includes a Computer phenotype, using the caveat that it's extremely hard DGKH to transplant the cells lots of cycles serially. (oaxacalibre.net)
  • Due to this, the sialic acid changes the structure and function of cell surface receptor proteins and regulates signal transduction and consequently, the cell phenotype. (uab.edu)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • However, certain perspectives maintain that this demarcation is artificial, since both processes act in complementary manners as far as actual tumor populations are concerned. (wikipedia.org)
  • 4 ]. A relationship between activated or lymphocytes and enzyme-linked immuno- memory-effector lymphocyte populations sorbent assay (ELISA) for CD117. (who.int)
  • Both EMT and CSC phenotypic changes are associated with tumor recurrence. (techscience.com)
  • The proposed approach addresses a long-standing challenge in current therapeutic approaches that utilize supraphysiological doses of growth factors to direct tissue research. (sciencedaily.com)
  • experimental approaches and assays available to assess developmental immunotoxicity. (cdc.gov)
  • Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)
  • Scintigraphy with indium-111 diethylenetriamine pentaacetic acid (DTPA) octreotide (In-111 DTPA Octr), or OctreoScan, localizes the primary carcinoid and eventual recurrences, as well as other neuroendocrine tumors, with high sensitivity and specificity. (medscape.com)