Tissue specific protein expressed sequence tags. Medical search. Frequent questions

ESTs may be used to identify gene transcripts, and were instrumental in gene discovery and in gene-sequence determination. (wikipedia.org)
Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. (wikipedia.org)
One can map ESTs to specific chromosome locations using physical mapping techniques, such as radiation hybrid mapping, Happy mapping, or FISH. (wikipedia.org)
In this respect, ESTs have become a tool to refine the predicted transcripts for those genes, which leads to the prediction of their protein products and ultimately of their function. (wikipedia.org)
Moreover, the situation in which those ESTs are obtained (tissue, organ, disease state - e.g. cancer) gives information on the conditions in which the corresponding gene is acting. (wikipedia.org)
Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the same mRNA of an organism. (wikipedia.org)
When the complete genome sequence of an organism is available and transcripts are annotated, it is possible to bypass contig assembly and directly match transcripts with ESTs. (wikipedia.org)
Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
Nearly 39% of the ESTs were predicted to encode proteins of fungal origin and among these, 162 ESTs were predicted to contain N-terminal signal peptides (SPs) in their deduced open reading frames (ORFs). (biomedcentral.com)
Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)
6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
The more recent duplication event in particular would be expected to result in many paralogous pairs of genes differing by relatively few sequence differences, thus complicating gene identification using ESTs. (biomedcentral.com)

EST approaches have largely been superseded by whole genome and transcriptome sequencing and metagenome sequencing. (wikipedia.org)

Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)

After Blast analysis within GenBank, 64% could be identified, 21% were homologous to unannotated expressed sequence tag (EST) or genomic sequences, and 15% were novel. (appliedbioinfo.com)
Large scale gene analysis of most organisms is hampered by incomplete genomic sequences. (biomedcentral.com)

Gene expression analyses using NGS platforms were carried out in the vegetative and reproductive tissues such as shoot, root, mature leaf, ﬂower bud, young pod, seed and nodule by various groups which resulted in identification of several tissue-specific transcripts. (intechopen.com)
For a selected subset of CEAPs and candidate effectors, semiquantitative RT-PCR showed that these transcripts were either expressed constitutively in both in vitro and in planta or induced during plant infection. (biomedcentral.com)
Database information indicates expression of SPRN in embryo, brain and retina of mouse and rat, hippocampus of human, and in embryo and retina of zebrafish, and we directly confirmed a strikingly specific expression of the mammalian (human, mouse, rat) transcripts in whole brain. (nih.gov)

Soybean has a large (1115 Mbp) genome that has yet to be fully sequenced. (biomedcentral.com)
Such EST data form a valuable foundation for the understanding of the gene composition and genomic biology of yet-to-be fully sequenced genomes [ 3 ]. (biomedcentral.com)

The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
The enzyme deficiencies lead to the accumulation of mucopolysaccharides in the lysosomes of the cells in the connective tissue and to an increase in their excretion in the urine. (medscape.com)
16q24.3 (Morquio syndrome): The deficiency of enzymes in Morquio syndrome type A or type B leads to the accumulation of keratan sulfate and chondroitin-6-sulfate in the connective tissue, the skeletal system, and the teeth. (medscape.com)

The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli . (hindawi.com)
The rabbits were immunized with the purified recombinant protein. (hindawi.com)
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
FDX1 (NP_004100.1, 85 a.a. ~ 183 a.a) partial recombinant protein with GST tag. (sigmaaldrich.com)

One way to generate GFP tagged proteins is through small epitope tag systems such as split-GFP (Ghosh 2000). (citiesofdata.org)
The Pur alpha-Rbcomplexes contain a form of Pur alpha with extensive post-syntheticmodification, as demonstrated following expression of Pur alpha cDNA fusedto a 9-amino acid epitope tag. (embl-heidelberg.de)

They may be represented in databases as either cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand. (wikipedia.org)
The presence of a stop codon in exon 13 generates two families of Sec31B gene products (each displaying additional patterns of mRNA splicing): a group of full-length proteins (hereafter referred to as Sec31B-F) and also a group of truncated proteins (hereafter referred to as Sec31B-T), distinguished by their utilization of exon 13. (biologists.com)
ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
The human coding sequence of MYH16 has a frameshift mutation that prevents translation of the functional product. (johnhawks.net)
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
This result together with some common structural features led to the suggestive hypothesis of a possible functional link between mammalian PrP and Sho proteins. (nih.gov)
We will now focus on establishing an immune (i.e. serological) response within cancer patients and attempt to characterise the functional significance of the proteins within individual tumours. (longdom.org)
You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
The large subunit interacts with the smaller subunit (sfGFP11), which can be inserted as a small fusion tag within the protein of interest, forming a functional GFP. (citiesofdata.org)
Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
Functional annotation of gene sequences indicate functional classifications are not randomly distributed among gene families containing few or many genes. (biomedcentral.com)
Pur-alpha can influence viral interaction through functional associations, for example with the Tat protein and TAR RNA of HIV-1, and with large T-antigen and DNA regulatory regions of JC virus. (embl-heidelberg.de)

Thirteen Arabidopsis and six rice NAC transcription factors have been shown to contain an α-helical transmembrane motif in the far C-terminal region which anchors the NAC protein to intracellular membranes rendering them inert. (biomedcentral.com)
Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
When expressed as a fusion protein with eYFP in cultured cells, Sec31B-F associates with the endoplasmic reticulum and with vesicular-tubular clusters, displays restricted intracellular movement characteristic of COPII vesicle dynamics, co-distributes on organelles with Sec13, Sec31A and Sec23 (markers of the COPII coat), and concentrates with ts045-VSV-G-CFP (VSV-G) when examined early in the secretory pathway or after temperature or nocodazole inhibition. (biologists.com)
Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)
Amyloidosis is a clinical disorder caused by extracellular and/or intracellular deposition of insoluble abnormal amyloid fibrils that alter the normal function of tissues. (medscape.com)

The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)

Advances in genomic sequencing technology are transforming medical discovery, enabling researchers to conduct comprehensive explorations across whole genomes for individuals and populations. (cdc.gov)

Eleven candidate effector genes were identified based on features common to characterized fungal effectors, i.e. they encode small, soluble (lack of transmembrane domain), cysteine-rich proteins with a putative SP. (biomedcentral.com)
Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)

To date, a number of fruit-specific promoters have been isolated and characterized from various plant species. (biomedcentral.com)
Phylogenetic comparisons to Brachypodium, rice, and Arabidopsis NAC proteins indicate that the barley NAC family includes members from all of the eight NAC subfamilies, although by comparison to these species a number of HvNACs still remains to be identified. (biomedcentral.com)
Although this protocol is specific for transgenic Aedes aegypti, the protocol may also be used for other mosquito species or other experimental treatment conditions, with the caveat that certain biological contexts may require special adaptations. (bvsalud.org)

In an effort to reduce the number of expressed sequence tags for downstream gene discovery analyses, several groups assembled expressed sequence tags into EST contigs. (wikipedia.org)
Extensive analyses of transcriptome have been carried out in chickpea, which is the third most important legume valued as a source of dietary protein and micronutrients. (intechopen.com)
Sequence analyses of unigenes revealed four potential groups: hydrolases, cell envelope associated proteins (CEAPs), candidate effectors and other proteins. (biomedcentral.com)
We report evidence from cDNA isolation and expression analysis as well as analyses of genome, expressed sequence tag (EST), cDNA and expression databases for a new gene named SPRN (shadow of prion protein). (nih.gov)

The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)

In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. (nih.gov)
Genes that showed very low expression patterns in normal tissues were then tested against a range of cancer types. (longdom.org)
These small subunit tags create fluorescent signals that matched the localization patterns of the wild-type protein in the gonad. (citiesofdata.org)
Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
Each of the four different nucleobases are characterized by distinct edge-specific distribution patterns of their respective hydrogen bond donor and acceptor atoms, complementarity with which, in turn, define the hydrogen bonding patterns involved in base pairing. (wikiversity.org)
They involve specific hydrogen bonding patterns corresponding to their respective Watson-Crick edges, and are considered as Canonical Base Pairs. (wikiversity.org)
[ 3 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)

These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
Transcriptome analysis has facilitated the development of molecular markers such as simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs) and potential intron polymorphisms (PIPs) that are being used to expedite the chickpea breeding programmes. (intechopen.com)
Entrez is NCBI's primary text search and retrieval system that integrates the PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, structure, gene, genome, genetic variation and gene expression. (nih.gov)
The BioProject database is a searcheable collection of complete and incomplete (in-progress) large-scale molecular projects including genome sequencing and assembly, transcriptome, metagenomic, annotation, expression and mapping projects. (nih.gov)
Conserved Domains is a database of protein domains represented by sequence alignments and profiles for protein domains conserved in molecular evolution. (nih.gov)
To date, molecular dynamics simulations focus on protein-protein and protein-ligand interactions, but there are currently no studies for simultaneous ligand exploration between two competing proteins. (biomedcentral.com)
Three explanatory types of data can be obtained from the current study: (i) the dynamics of multiple binding sites, (ii) competition between two proteins for a ligand, and (iii) the intrinsic molecular components involved in the competition. (biomedcentral.com)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
By cataloguing the molecular processes by which cells maintain and modify protein levels, proteomic studies offer another dimension of information that may help advance our understanding of health and disease. (cdc.gov)

The ancillary domain, which mediates binding to ECM components and specific protease substrates, consists of a conserved central thrombospondin type 1 sequence repeat motif (TSR), a cysteine-rich domain and a spacer domain. (encyclopedia.pub)
Cysteine-rich PR proteins, such as non-specific lipid transfer proteins (nsLTPs) and metallocarboxypeptidase inhibitors, are candidates for the sequestration of metals. (uni-bayreuth.de)

In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. (wikipedia.org)
sequenced 178 clones from a rabbit muscle cDNA library. (wikipedia.org)
Almost 2,000 expressed sequence tag (EST) cDNA clones were sequenced to analyze gene expression in control and Cd-treated leaf trichomes. (uni-bayreuth.de)

Extraction and Solubilization of Proteins for Proteomic Studies - Richard M. Leimgruber 2. (nhbs.com)

B-cell translocation gene 4, cullin 1, MCF.2 transforming sequence, a locus similar to snail soma ferritin, and three unidentified genes were, indeed, preferentially expressed in the oocyte, even though most were also highly expressed in testis. (appliedbioinfo.com)
Sec31B is widely expressed and enriched in cerebellum and testis. (biologists.com)

Over the last two decades, several laboratories have used a wide range of techniques encompassing expressed sequence tag (EST) analysis, serial analysis of gene expression (SAGE), microarray and next-generation sequencing (NGS) technologies for analysing the chickpea transcriptomes. (intechopen.com)
We developed a microarray-based approach for the systematic discovery of tissue-specific genes and, by applying it to Foxo3 ovaries and other samples, defined a surprisingly large set of ovarian factors (n = 348, approximately 1% of the mouse genome). (appliedbioinfo.com)
Methods and Results: We have performed a bioinformatics screen using microarray and expressed-sequence tag databases to identify potential new CTAs. (longdom.org)

Among these tick salivary proteins are lipocalins, antiparallel beta-barrel proteins that sequester small molecules. (biomedcentral.com)
Aided by state-of-the-art, high-throughput computational methods, the current study simulated and analyzed the dynamics of competitive histamine binding at the tick-host interface using the available crystal structures of both the tick salivary lipocalin histamine-binding protein from Rhipicephalus appendiculatus and the human histamine receptor 1. (biomedcentral.com)
Salivary gland transcriptomes have revealed that ticks, as obligate hematophagous (blood feeding) arthropods, secrete many protein families at the injury site to counteract host physiological responses - antagonizing inflammation is one example of the mechanisms ticks employ. (biomedcentral.com)
The crystal structure of one of these tick salivary Ra-HBPs (Ra-HBP2) revealed a few structural deviations from the archetypal lipocalin protein family, that it sequesters two histamine molecules, and also causes an inhibition of guinea-pig ileum to contract by competing with H1R/H2R for histamine binding [ 6 ]. (biomedcentral.com)
Here, we generated two knockout (KO) mosquito lines by CRISPR/Cas9 to functionally characterize D7L1 and D7L2, two abundantly expressed salivary proteins from the yellow fever mosquito vector Aedes aegypti. (bvsalud.org)
The absence of D7 proteins in the salivary glands of KO mosquitoes was confirmed by mass spectrometry, enzyme-linked immunosorbent assay, and fluorescence microscopy of the salivary glands with specific antibodies. (bvsalud.org)
We also investigated the role of D7 salivary proteins in Plasmodium gallinaceum infection and transmission. (bvsalud.org)
Aedes aegypti, the vector of yellow fever and dengue, expresses two D7 long-form salivary proteins: D7L1 and D7L2. (bvsalud.org)

This gene product has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific. (sigmaaldrich.com)

Thereafter, the fungus switches to the necrotrophic phase that is associated with the production of thin secondary hyphae that ramify intra- and inter-cellularly, killing and macerating host tissues by hydrolytic enzymes ahead of infection. (biomedcentral.com)

The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. (biomedcentral.com)

Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. (betalifesci.com)
To regulate tissue-specific fluorescence of target proteins, the large subunit (sfGFP1-10) is definitely constitutively expressed under the regulation of a temporally and/or spatially limited promoter. (citiesofdata.org)

For instance women with two chromosomes have one of these silenced by the same methylation tag such that they have one active X chromosome as have men. (sciencedaily.com)
Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. (origene.com)

Our results revealed that the trichome-specific transcriptome approach is a powerful tool to investigate the defensive functions of trichomes against both abiotic and biotic stress. (uni-bayreuth.de)

The biological and pathological functions of ADAMTS proteases are determined broadly by their respective substrates and their interactions with proteins in the pericellular and extracellular matrix. (encyclopedia.pub)
However, the four ADAMTS proteases show differences in the number and localization of predicted sites for posttranslational modifications, such as N-glycosylation ( http://www.cbs.dtu.dk/services/NetNGlyc/ ), O-fucosylation, and furin processing which could specify individual substrates, define protein-protein interactions, or govern autocatalytic properties that collectively distinguish these four ADAMTS proteases from each other. (encyclopedia.pub)
There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. (nhbs.com)
Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)

Typical NAC transcription factor, with N-terminal NAC domain, consisting of five conserved subdomains, A-E. The C-terminal part of the protein is more variable and contains the transcriptional activation or repressing region. (biomedcentral.com)
Over-expressed MS1 co-localized with actin in H9c2 cells and altered expression of genes of the myocardin-related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathways and in addition the apoptosis repressor with caspase recruitment domain (Nol3) gene. (westminster.ac.uk)

In response to tissue damage or a hypersensitivity reaction, histamine is released by mast cells and basophils that then bind to its native receptors (histamine receptors, H1R and/or H2R) to facilitate repairing agents or mediators of the immune response arriving at the injury site [ 1 ]. (biomedcentral.com)
To isolate bovine oocyte marker genes, we performed suppressive and subtractive hybridization between oocytes and somatic tissues (i.e., intestine, lung, muscle, and cumulus cells). (appliedbioinfo.com)
Comparative profiling of other tissues, including microdissected oocytes and somatic cells, revealed distinct gene classes and provided new insights into oogenesis and ovarian function, demonstrating the utility of our approach for tissue-specific gene discovery. (appliedbioinfo.com)
This modification (methylation) is important not only in gene expression but also in ensuring that there is the right balance in the level of expression of proteins in different cells. (sciencedaily.com)
Estrogen withdrawal or treatment with the established anticancer drug doxorubicin cause the methyl groups to be removed from regulatory regions of specific genes within tens of minutes in human breast cancer cells. (sciencedaily.com)
In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. (betalifesci.com)
As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. (betalifesci.com)
Another limitation of this technology is definitely that GFP fusions illuminate the proteins of interest in all cells of an organism that communicate the target protein, which can be problematic when attempting to study the role of a protein in a specific cells. (citiesofdata.org)
These split-GFP systems also have the advantage of creating cells specific reporter tags. (citiesofdata.org)
To test for this, apelin-55 was applied exogenously to HEK293A cells overexpressing proprotein convertase subtilisin kexin 3 (PCSK3), the only apelin processing enzyme identified thus far, and to differentiated 3T3-L1 adipocytes, which endogenously express apelin, PCSK3 and other proprotein convertases. (phoenixpeptide.com)
In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)
Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
TFs regulate genes to ensure they're expressed in proper sequence and at the right time in cells. (ddw-online.com)
The breakthrough was in realizing that we could leverage deep learning and massive datasets of tissue-level TF binding profiles to understand how TFs regulate target genes in individual cells through specific signals," Xie said. (ddw-online.com)
Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separations - Regis Dieckmann, Yohanne Coute, Denis Hochstrasser, Jean-Jacques Diaz and Jean-Charles Sanchez 11. (nhbs.com)
This is because expression of the transgene is controlled by a promoter specific to lactating mammary cells. (sciencelearn.org.nz)
The human Pur factor binds strongly to a sequence element repeated withinzones of initiation of DNA replication in several eukaryotic cells. (embl-heidelberg.de)
These proteins either arise from proteins expressed by cells at the deposition site (localized), or they precipitate systemically after production at a local site (systemic). (medscape.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

During the transition, C. truncatum probably secretes a range of proteins to establish a compatible interaction with its host, including some that may exclusively be involved in switching the pathogen to the necrotrophic phase. (biomedcentral.com)

Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
By comparing the sequences of humans and other primates, Stedman's team estimated that MYH16 had lost its function in human ancestors around 2.4 million years ago. (johnhawks.net)
With the sequencing of the human genome the general public has become very aware that the answer to many diseases lies in our DNA. (sciencedaily.com)
Brain is the most important tissue in human speciation. (karger.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
A: An alignment of amino acid sequences of rat preproapelin with human and bovine preproapelin. (phoenixpeptide.com)
B: An alignment of amino acid sequences of human apelin and angiotensin II. (phoenixpeptide.com)
Differentially regulated genes were validated at the level of gene and protein expression, with genes identified as estrogen-regulated in human confirmed as estrogen-dependent in young estrogen depleted mice in vivo . (biomedcentral.com)
Scientists at AgResearch in New Zealand have successfully produced healthy transgenic cows that make modified milk or human therapeutic proteins in their milk. (sciencelearn.org.nz)
Since 2000, scientists at AgResearch have been successfully producing transgenic cows that make modified milk or produce therapeutic proteins to treat human diseases. (sciencelearn.org.nz)
Association of human Pur alpha with the retinoblastoma protein, Rb,regulates binding to the single-stranded DNA Pur alpha recognitionelement. (embl-heidelberg.de)
Human Pur alpha, expressed as a glutathioneS-transferase fusion protein, specifically binds to the hypophosphorylatedform of Rb with an affinity as high as that of SV40 large T-antigen. (embl-heidelberg.de)
Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
Studies of the human proteome have enabled scientists to track protein synthesis, modification, and degradation over time and across different cell types. (cdc.gov)
In 2014, scientists developed a draft map of the human proteome , which catalogued proteins encoded by over 17,000 human genes, or about 84% of all protein-coding genes in the human genome. (cdc.gov)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)

very easily with the smaller subunit, while constitutively expressing the larger subunit from another locus. (citiesofdata.org)
Therefore, our study shows that this approach could be utilized for tissue-specific GFP manifestation from an endogenous locus. (citiesofdata.org)
However, this was performed by transgenic integration of both parts of the system, outside the endogenous locus, making it unsuitable for Rabbit Polyclonal to CROT adaptation of CRISPR/Cas9 technology, while potentially adding confounding variables to studying protein function (observe discussion). (citiesofdata.org)
This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. (sigmaaldrich.com)

They looked at more than 1100 base pairs of sequence from a chimpanzee, orangutan, macaque, and a dog, and they found that the coding sequence in humans has a small number of unexpected amino-acid-coding changes-changes that wouldn't be likely if the gene product was still being maintained by selection. (johnhawks.net)
The purine-rich element binding (Pur) protein family protein consists PURalpha/beta/gamma in humans. (embl-heidelberg.de)
[ 4 ] In humans, about 23 different unrelated proteins are known to form amyloid fibrils in vivo. (medscape.com)

Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. (biomedcentral.com)
The use of tissue-specific promoters is critical for producing transgenic crops with improved economically important tissues, such as the fruits in citrus. (biomedcentral.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Find out more about Transgenic cows making modified milk and Transgenic cows making therapeutic proteins . (sciencelearn.org.nz)
Researchers use ear tags and microchips to identify transgenic cows and their calves. (sciencelearn.org.nz)

Reverse transcription-PCR (RT-PCR) analysis demonstrated that antipathogenic T-phylloplanin-like proteins, glutathione peroxidase and several classes of pathogenesis-related (PR) proteins were expressed specifically or dominantly in trichomes. (uni-bayreuth.de)
We investigated the expression profile of several candidates in the oocyte and a panel of gonadal and somatic tissues by reverse transcription-polymerase chain reaction. (appliedbioinfo.com)
Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
In recent years, research in the regulatory roles of members of the plant-specific NAC ( N AM, A TAF-1,2, C UC) transcription factor family has increased considerably. (biomedcentral.com)
This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)

and a C-terminal peptide predicted to be a signal sequence for glycophosphotidylinositol (GPI)-anchor attachment. (nih.gov)

For example, a protein that is active in a nerve cell is not expressed in the liver. (sciencedaily.com)
In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. (betalifesci.com)
Significant expression of IL-33 was seen in mouse liver and lung tissues determined with the anti-IL-33. (hindawi.com)

Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. (enquirebio.com)

Plays a role in the degradation of Extracellular domain matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. (enquirebio.com)
All types of amyloid consist of one major fibrillar protein that defines the type of amyloid. (medscape.com)

In a phylogenetic analysis of coleopteran and lepidopteran arylsulfatases, the P. chrysocephala GSSs formed a cluster within a coleopteran-specific sulfatase clade distant from the previously identified GSSs of the diamondback moth, Plutella xylostella , suggesting an independent evolution of GSS activity in ermine moths and flea beetles. (nature.com)
Until now, that process had been limited to tissue-level analysis. (ddw-online.com)
Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)
Analysis of these sequences revealed single base substitutions and single base indels are the most frequently observed form of sequence variation between genes within families in the dataset. (biomedcentral.com)

In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (nih.gov)
In contrast to ancestral clade 2.3.4 H5 proteins, novel clade 2.3.4.4 H5 proteins bind to fucosylated sialosides because of substitutions K222Q and S227R, which are unique for highly pathogenic influenza virus H5 proteins. (cdc.gov)

Ticks counteract host inflammatory responses by secreting proteins from their saliva that compete for histamine binding. (biomedcentral.com)
Excessive immune and inflammatory responses can cause a wide spectrum of pathologies, thus resulting in either cell lesions or tissue damage. (frontiersin.org)
Polymorphisms that slightly vary native peptides or inflammatory processes set the stage for abnormal protein folding and amyloid fibril deposition. (medscape.com)

A number of characterized NAC proteins have a conserved transmembrane motive, TM, in the far C-terminal region. (biomedcentral.com)

The 162 sequences could be assembled into 122 tentative unigenes comprising 32 contigs and 90 singletons. (biomedcentral.com)

Apelin peptides are cognate ligands for the apelin receptor, a G-protein-coupled receptor (GPCR). (phoenixpeptide.com)

Using live imaging we were able to detect germline fluorescence of all of the tagged proteins in the germline. (citiesofdata.org)

In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. (wikipedia.org)

In 1991, Adams and co-workers coined the term EST and initiated more systematic sequencing as a project (starting with 600 brain cDNAs). (wikipedia.org)

However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. (betalifesci.com)
Only 10% of amyloidosis deposits consist of components such as glycosaminoglycans (GAGs), apolipoprotein-E (apoE), and serum amyloid P-component (SAP), while nearly 90% of the deposits consist of amyloid fibrils that are formed by the aggregation of misfolded proteins. (medscape.com)

HA proteins bind to sialoside receptors on the host cell surface. (cdc.gov)
These proteins bind and counteract hemostatic agonists such as biogenic amines and leukotrienes. (bvsalud.org)

A first challenge is that tissue provenance of EST libraries is described in plain English in dbEST. (wikipedia.org)
This makes it difficult to write programs that can unambiguously determine that two EST libraries were sequenced from the same tissue. (wikipedia.org)
In many organisms, such as soybean, the best source of sequence information is the existence of expressed sequence tag (EST) libraries. (biomedcentral.com)
Many EST libraries were constructed from RNA extracted from various genetic backgrounds, thus gene identification from these sources is complicated by the existence of both gene and allele sequence differences. (biomedcentral.com)

Anatomy15

Organisms20

Chemicals and Drugs35

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes59

Disciplines and Occupations4

Technology, Industry, Agriculture4

Information Science13

Health Care2

ESTs13

Superseded by whole genome1

Differentially expressed4

Genomic sequences2

Transcripts3

Fully sequenced2

Connective tissue3

Recombinant protein6

Epitope tag2

MRNA3

Functional10

Intracellular5

Full-length proteins1

Genomes1

Putative3

Species3

Analyses4

Various tissues1

Patterns10

Molecular11

Cysteine-rich2

Clones3

Proteomic Studies1

Testis2

Microarray3

Salivary8

Different tissues1

Hydrolytic enzymes1

Vegetative1

Constitutively expressed2

Chromosome2

Transcriptome1

Interactions4

Transcriptional2

Cells19

Pathogen1

Human17

Locus4

Humans3

Transgenic5

Transcription7

Signal sequence1

Liver3

Regulatory1

Fibrillar2

Analysis4

Substitutions2

Inflammatory3

Transmembrane1

Unigenes1

Peptides1

Fluorescence1

Genetics1

Systematic1

Aggregation2

Bind2

Libraries4