Tissue antigens thy 1. Medical search. Frequent questions

Thy-1 or CD90 (Cluster of Differentiation 90) is a 25-37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. (wikipedia.org)
The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons. (exbio.cz)
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. (exbio.cz)
CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. (exbio.cz)
Here we identify a population of lung-resident ILCs in mice and humans that expressed the alloantigen Thy-1 (CD90), interleukin 2 (IL-2) receptor a-chain (CD25), IL-7 receptor a-chain (CD127) and the IL-33 receptor subunit T1-ST2. (nih.gov)

The 162aa (murine, 161 for human) Thy1 precursor has 19 amino acid (aa 1-19) signal sequence and 31 amino acid (aa 132-162) C-terminal transmembrane domain that is present in pro form but removed when transferring the 112 amino acid (aa 20-131) mature peptide to GPI anchor which would attach through the aa 131. (wikipedia.org)
There have been some reports of Thy1 monoclonal antibodies cross reacting with some cytoskeletal elements: anti Thy-1.2 with actin in marsupial, murine, and human cells and anti Thy-1.1 with vimentin, and were suggested to be due to sequence homology by studies done more than 20 years back. (wikipedia.org)
Corresponding THY1 RNA-seq data are presented for the same tissues. (atlasantibodies.com)
Stimulation of colonic leukocytes with IL-23 induced the production of IL-17 and interferon-gamma exclusively by innate lymphoid cells expressing Thy1, stem cell antigen 1 (SCA-1), retinoic-acid-related orphan receptor (ROR)-gammat and IL-23R, and these cells markedly accumulated in the inflamed colon. (ox.ac.uk)

Thy-1, like many other GPI anchored proteins can be shed by special types of Phospholipase C e.g. (wikipedia.org)
Thy-1 is one of the most heavily glycosylated membrane proteins with a carbohydrate content up to 30% of its molecular mass. (wikipedia.org)
The increased intestinal permeability was accompanied by augmented expression of genes marking immune cell infiltration and encoding key proteins for immune cell adhesion, antigen presentation, and cytokine signaling in the orbital tissue. (frontiersin.org)
Different MHC molecular forms, be they class I or II, can bind, and thus present, a different set of peptides.Apr 19, 2020 · Data for PD-L1 and MHC I are defined by median fluorescent intensity (MFI) of all cells, given the uniform induction of these proteins (online supplementary figure 1). (projekthouse.eu)
The expression of B7 proteins on an antigen-presenting cell is induced by pathogens during the innate response to an infection. (nih.gov)
Effector T cells act back to promote the expression of B7 proteins on antigen-presenting cells, creating a positive feedback loop that amplifies the T cell response. (nih.gov)
proteins in HSV-1-infected cells. (microbiologyresearch.org)

Additionally, we introduce the prospective isolation of DPSCs using specific cell surface markers: low-affinity nerve growth factor and thymocyte antigen 1. (elsevierpure.com)

The complex formed by the binding of antigen and antibody molecules. (lookformedical.com)
63. MHC Class II molecules are a class of major histocompatibility complex (MHC) molecules normally found only on professional antigen-presenting cells such as dendritic cells, mononuclear phagocytes, some endothelial cells, thymic epithelial cells, and B cells. (projekthouse.eu)
Hence, the effects of the inhibitors of signalling molecules at MCP-1 release were not studied after IFN-γ stimulation of human monocytes and neutrophils. (cgrpreceptor.com)
mAbs specific for immunoregulators have sometimes proven useful in establishing the importance of such molecules in immunity against experimental tumors ( 1 - 3 ). (silverchair.com)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb90844 antibody. (atlasantibodies.com)
Detect Thy-1 using this Anti-Thy-1 Antibody, clone F15-42-1, Phycoerythrin conjugated validated for use in FC. (scientificlabs.co.uk)
Antibody not tested in fixed tissue. (scientificlabs.co.uk)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
The 53-2.1 monoclonal antibody specifically binds to the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. (bdbiosciences.com)
The 53-2.1 antibody has been reported not to crossreact with Thy-1.1 (e.g. (bdbiosciences.com)
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye ( FLUORESCENT ANTIBODY TECHNIQUE , DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody ( FLUORESCENT ANTIBODY TECHNIQUE , INDIRECT). (lookformedical.com)
The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES . (lookformedical.com)
Group of diseases mediated by the deposition of large soluble complexes of antigen and antibody with resultant damage to tissue. (lookformedical.com)
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
gG polypeptides were present in purified HSV-1 virions and were targets for antibody-mediated complement-dependent virus neutralization. (microbiologyresearch.org)

Antibodies used for cell surface labeling were purchased from BD PharMingen (San Diego, CA) and included fluorescein isothiocyanate (FITC)-labeled anti-Thy-1.2, anti-Gr-1, and anti-bromodeoxyuridine (BrdU), phycoerythrin-labeled anti-CD4, anti-CD3, and anti-NK1.1. (neuromantics.net)
Biotinylated anti-CD3ε, anti-T-cell receptor (TCR)αβ, anti-Thy-1.2, and anti-Mac-1 (CD11b) were detected by streptavidin-Cy5 (Jackso. (neuromantics.net)

Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. (wikipedia.org)
Thy-1 was discovered by Reif and Allen in 1964 during a search for heterologous antisera against mouse leukemia cells, and was demonstrated by them to be present on murine thymocytes, on T lymphocytes, and on neuronal cells. (wikipedia.org)
It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). (wikipedia.org)
The discovery of Thy-1 in mice and humans led to the subsequent discovery of many other T cell markers, which is very significant to the field of immunology since T cells (along with B cells) are the major cellular components of the adaptive immune response. (wikipedia.org)
The composition of Thy-1 carbohydrate moieties varies considerably between different tissues or even among cells of the same lineage at different stages of differentiation: e.g., galactosamine only in brain Thy-1, sialic acid in thymic Thy-1 in far excess than brain Thy-1, that too increasing in parallel with T cell maturation. (wikipedia.org)
Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
The influx of macrophages and accumulation of T-cells and myofibroblast were quantified in orbital connective tissue. (frontiersin.org)
Innate lymphoid cells (ILCs), a heterogeneous cell population, are critical in orchestrating immunity and inflammation in the intestine, but whether ILCs influence immune responses or tissue homeostasis at other mucosal sites remains poorly characterized. (nih.gov)
The polyA Spin™ Kit is a rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ (mRNA) from cytoplasmic or "total" RNA isolated from eukaryotic tissue and cells. (neuromantics.net)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
The immune system is comprised of highly specialized q The findings and conclusions in this report are those of the authors and cells, tissues, and organs that give the human body the do not necessarily represent the views of the Agency for Toxic Substances and Disease Registry. (cdc.gov)
They are activated on the surface of antigen -presenting cells, which mature during the innate immune responses triggered by an infection. (nih.gov)
We also consider how innate immune responses determine the nature of adaptive responses by stimulating helper T cells to differentiate into either T H 1 or T H 2 effector cells. (nih.gov)
5. Moritz DR, Rodewald H, Gheyselinck J, Klemenz R. The IL-1 receptor-related T1 antigen is expressed on immature and mature mast cells and on fetal blood mast cell progenitors. (southernbiotech.com)
13. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. (southernbiotech.com)
It has previously been shown that scrib KD MDCK cells are eliminated when co-cultured with wild-type MDCK cells through cell death and delamination (see ref. 23 and Supplementary Fig. 1a and Supplementary Movie 1 , left), while monocultures of scrib KD cells are viable (see ref. 23 and Supplementary Fig. 1b and Supplementary Movie 1 , right). (nature.com)
The Helicobacter pylori Urease Virulence Factor Is Required for the Induction of Hypoxia-Induced Factor-1α in Gastric Cells. (cmcmed.cl)
AT2 Receptor Mediated Activation of the Tyrosine Phosphatase PTP1B Blocks Caveolin-1 Enhanced Migration, Invasion and Metastasis of Cancer Cells. (cmcmed.cl)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
immune destruction of insulin-pro- vestigate the relationship of VDR gene ducing beta cells by T-cell infiltration polymorphism to the risk of type 1 DM DNA isolation and PCR experiments of the pancreatic islets [1]. (who.int)

The 25-kDa core protein (excluding the heavy glycosylation) of rodent Thy-1 is 111 or 112 amino acids in length, and is N-glycosylated at three sites (In contrast to only two glycosylation sites for human Thy-1). (wikipedia.org)
In this regard it has yet another historic association: Thy-1 happens to be the first glycoprotein in which cell type specificity of variant glycosylation on an invariant protein was demonstrated. (wikipedia.org)
The expression levels of dentin sialo-phosphoprotein (DSPP), dentin matrix protein-1 (DMP1), enamelysin/matrix metalloproteinase 20 (MMP20) and phosphate-regulating gene with homologies to endopeptidases on X chromosome (PHEX) were analysed using RT-PCR. (scielo.br)
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. (atlasantibodies.com)
Signal 1 is provided by a foreign peptide bound to an MHC protein on the surface of the presenting cell. (nih.gov)
A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. (bvsalud.org)
From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C. (bvsalud.org)

A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue. (lookformedical.com)
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. (lookformedical.com)
Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. (lookformedical.com)
Systemic sclerosis represents a spectrum of connective tissue disorders, characterized by chronic and debilitating fibrosis of the skin and internal organs, most notably the lungs, kidney, cardiovascular system and gastrointestinal tract [ 1 ]. (biomedcentral.com)

Collectively, our results demonstrate a critical role for lung ILCs in restoring airway epithelial integrity and tissue homeostasis after infection with influenza virus. (nih.gov)

Analysis of Differencial glycosylation of Thy-1 from brain and thymus showed that all the complex N-linked structures differed between the two forms, superimposed upon a site specific common core. (wikipedia.org)

Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. (wikipedia.org)

Graves' disease (GD), characterized by TSH-receptor stimulating antibodies and increased thyroid hormone serum levels, is an autoimmune disease affecting roughly 3% of the general population ( 1 ). (frontiersin.org)
The antigen is extracted from the cell in an appropriate lysis buffer, and antibodies are added to the lysate to allow formation of the immune complex. (neuromantics.net)
However, it also antagonizes the effects of anti-Gr-1 depleting antibodies. (frontiersin.org)

Asbestos induced mesothelioma mouse model in which expression of the Simian Virus 40 large T antigen (SV40 TAg) is controlled by mesothelin tissue‑specific promoter. (ncard.org.au)
This includes the expression of distinct antigens on subsets of MSCs, which was utilized recently to define and separate functionally different subsets from bulk MSCs. (hindawi.com)
Western analysis was performed to determine expression of maspin and survivin in the tumor tissues. (biomedcentral.com)
Tissue expression and subcellular localization of the pro-survival molecule Bcl-w. (southernbiotech.com)

The signaling events initiated by the binding of peptide-MHC complexes to T cell receptors (signal 1). (nih.gov)

Orbital pre-adipocytes and fibroblasts also express the TSH receptor, resulting in expanded retro-orbital tissue and causing exophthalmos and limited eye movement. (frontiersin.org)
The T cell receptor does not act on its own to transmit signal 1 into the cell. (nih.gov)
ABSTRACT Reported associations between vitamin D receptor (VDR) polymorphism and type 1 diabetes mellitus vary across ethnic groups. (who.int)

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. (biolegend.com)
A recombinant vaccinia virus expressing herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) has been constructed. (microbiologyresearch.org)

Graves' disease is classified as an autoimmune disorder, one of a large group of conditions that occur when the immune system attacks the body's own tissues and organs. (medlineplus.gov)

Within the microenvironment of a tumor, TAMs can play a "pro-cancer" role that suppresses the host immune system, enables tumor tissue repair, and promotes tumor growth, or they can play an "anti-cancer" role by triggering strong and focused immune system attacks on tumor tissue. (holisticprimarycare.net)

Meaning: major histocompatibility complex.Dec 24, 2022 · 11.11A: MHC Polymorphism and Antigen Binding. (projekthouse.eu)

A major breakthrough in cancer therapy is immune checkpoint inhibitor (CPI)-based immunotherapy which has been approved by the FDA for the treatment of multiple types of cancers ( 1 ). (frontiersin.org)

ProcedureOne procedure is provided for processing fresh, frozen, RNAlater-preserved, and/or FFPE tissues. (neuromantics.net)

Dr. Gunn, a co-founder of InspireHealth , Canada's leading integrative cancer center, will highlight the role of tumor associated macrophages (TAMs) in determining the growth potential (or lack thereof) of tumor tissue. (holisticprimarycare.net)

Inflammation and Regeneration , 37 (1), 1-10. (elsevierpure.com)
Some people develop bulging of the eyes caused by inflammation of tissues behind the eyeball and "pulling back" (retraction) of the eyelids. (medlineplus.gov)
The active 1,25(OH)2D form of vitamin D is a potent modulator of inflammation, and may play a role in turning off chronic inflammatory response. (trustedcarechiro.com)

In mice, there are two alleles: Thy-1.1 (Thy-1a, CD90.1) and Thy-1.2 (Thy-1b, CD90.2). (wikipedia.org)

The antigen Thy-1 was the first T cell marker to be identified. (wikipedia.org)
Macrophage influx was positively linked to the extent of T cell influx and fibroblast activation within GO-affected orbital tissues. (frontiersin.org)
Proliferating cell nuclear antigen was also expressed by myofibroblasts and pericytes in dcSSc skin while being absent in control skin. (biomedcentral.com)
Sternal biopsy was compatible with metastases from clear cell renal carcinoma ( Figure 1 ). (scirp.org)
1. Prepare a single-cell suspension from the lymphoid tissue of interest according to standard laboratory procedures. (bdbiosciences.com)
To activate a cytotoxic or helper T cell to proliferate and differentiate into an effector cell , an antigen-presenting cell provides two kinds of signals. (nih.gov)
If a T cell receives signal 1 without signal 2, it may undergo apoptosis or become altered so that it can no longer be activated, even if it later receives both signals ( Figure 24-62 ). (nih.gov)
This is one mechanism by which a T cell can become tolerant to self antigens. (nih.gov)
A) A mature antigen-presenting cell can deliver both signal 1 and 2 and thereby activate the T cell. (nih.gov)
The combined actions of signal 1 and signal 2 stimulate the T cell to proliferate and begin to differentiate into an effector cell by a curiously indirect mechanism. (nih.gov)
Cell Intrinsic and Extrinsic Mechanisms of Caveolin-1-Enhanced Metastasis. (cmcmed.cl)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

To prevent tissue detachment during antigen retrieval, unbaked slides must be baked for at least 30 to 120 minutes at 60°C . before putting into xylene for de-paraffinization. (tissuearray.com)

Helicobacter pylori infection [ 1 ]. (who.int)

The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). (wikipedia.org)
In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. (elsevierpure.com)
To 1 per effect is balanced through the use and human growth hormone ceilstinuslcuting ilornione. (stonecottagegardens.com)
But research progressed and different trials explore the clinical potential of human MSCs isolated from bone marrow but also from other tissues including adipose tissue. (hindawi.com)
A workshop cosponsored by the National Institute of Environmental Health Sciences and the studies and/or human reports (Table 1). (cdc.gov)
Moreover, rottlerin, when applied alone, enhanced MCP-1 secretion by human monocytes (Fig. 5c). (cgrpreceptor.com)
The treatment of human monocytes with IFN-γ yielded no significant changes in MCP-1 levels (Fig. 3c). (cgrpreceptor.com)
Altogether, the results of our experiments allowed us to suggest a possible scheme of signalling events involved in the enhancement of MCP-1 secretion triggered after combined stimulation of human neutrophils and monocytes GSK-3 inhibition with PAR2-cAP and IFN-γ (Fig. 6a,b). (cgrpreceptor.com)

This synergistic action of PAR2 agonist and IFN-γ on MCP-1 release apparently involves the activation of PI3 kinase and JAKs in neutrophils and monocytes. (cgrpreceptor.com)

The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa califomica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. (microbiologyresearch.org)

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections 1 and frozen tissue sections 2 . (biolegend.com)
2. Most TMA slides were not coated with an extra layer of paraffin (tissue cores can be easily seen on the glass). (tissuearray.com)

H5N1 virus infection (1). (cdc.gov)
The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG- VAC. (microbiologyresearch.org)
Fifth metatarsal tuberosity fractures (dancer's fracture) are additionally common and consist of an apophyseal avulsion fracture at the peroneus brevis tendon insertion hiv infection rates london [url=https://qsrbn.gov.ng/buy-online/Medex/]medex 1 mg buy cheap on-line[/url]. (ehd.org)

Site Specific Immunotherapy (SSI), is an emerging modality that involves injecting antigens to stimulate TAMS to shift into the anti-cancer mode. (holisticprimarycare.net)

Histopathologic and immunohistochemical evidence of embedded tissue sections stained with hematoxylin and avian influenza A (H5N1) virus in leopard lung. (cdc.gov)
The immunohistochemical study was negative for Thyroid Transcription Factor 1 (TTF-1), although it was not valued for vimentin, cytokeratin 8 and CD10 because of the small size of the lesion that did not allow an adequate study ( Figure 3 ). (scirp.org)

Using α-smooth muscle actin, the ED-A splice variant of fibronectin (ED-A FN) and Thy-1 to identify myofibroblasts, we demonstrated the presence of myofibroblasts in fibrotic dcSSc skin. (biomedcentral.com)
Like pericytes, myofibroblasts express alpha smooth muscle actin (α-SMA) and are strongly associated with fibrotic tissue [ 5 ]. (biomedcentral.com)

Thyroid-associated orbitopathy (TAO) is part of an autoimmune process that can affect the orbital and periorbital tissue, the thyroid gland, and, rarely, the pretibial skin or digits (thyroid acropachy). (medscape.com)

The inhibition of PI3K and JAKs reduced, but did not abolish, the enhanced MCP-1 secretion, which was induced after monocytes were treated with PAR2-cAP together with IFN-γ (Fig. 5a). (cgrpreceptor.com)
Rottlerin also synergized with PAR2-cAP in its action on MCP-1 secretion (Fig. 5b). (cgrpreceptor.com)
inhibitor SB203580 did not influence the increased MCP-1 secretion caused by either PAR2-cAP stimulation or combined application of PAR2-cAP and IFN-γ (Fig. 5a,b). (cgrpreceptor.com)

Two distinct cohorts of GO patients were studied, one of which was a unique cohort consisting of blood, fecal, and retro-orbital tissue samples. (frontiersin.org)
The subsequent translocation of bacterial compounds to the systemic circulation may aggravate inflammatory processes within the orbital tissue and, as a consequence, augment the proportion of activated myofibroblasts, which actively secrete extracellular matrix leading to retro-orbital tissue expansion. (frontiersin.org)
These findings warrant further exploration to assess the correlation between specific inflammatory pathways in the orbital tissue and the gut microbiota composition and may pave the way for new microbiota-targeting therapies. (frontiersin.org)
[ 6 ] The disease process is a primary orbitopathy in which the orbital and periocular soft tissues are primarily affected, with secondary effects on the eye. (medscape.com)
[ 1 , 2 , 3 ] Although the use of the term thyroid ophthalmopathy is pervasive, the disease process is actually an orbitopathy in which the orbital and periocular soft tissues are primarily affected with secondary effects on the eye. (medscape.com)

Anatomy21

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CD905

Thy14

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Characterization1

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