Tissue biological assay. Medical search. Frequent questions

Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
The in-vitro or ex-vivo assays can target the various biological mechanisms providing a limitless opportunities of claims support. (premiumbeautynews.com)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq , " Evaluation of Cellular Processes by In Vitro Assays ", Bentham Science Publishers (2018). (benthamscience.com)
In vitro sphere formation assays are a type of colony formation assay used to evaluate the clonogenic potential of CSCs and other stem cells. (bio-connect.nl)
Objectives: To investigate the potential role of a commercially available preparation of micronized Amniotic Membrane Allograft (AMA) (Amniofix, MiMedx, USA) in the biological augmentation of an in vitro model of rotator cuff repair with respect to cellular pro- liferation, collagen content and mechanical properties of the bone- tendon interface. (researchgate.net)
Evaluation of the efficacy of proposed agents in an in vitro model is convenient and allows assessment of the mechanical properties of the bone-tendon interface as well as the effect on fibroblast proliferation and collagen content of the tissue. (researchgate.net)

Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression. (muni.cz)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Can be readily automated as a high-throughput assay for thousands of samples per day. (immpact-international.org)

A TdT-mediated dUTP nick-end labeling (TUNEL) assay was used to identify cell apoptosis. (frontiersin.org)

Cuvette or 96-well plate assay formats possible. (immpact-international.org)
Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. (bioassaysys.com)

The lower limit of reliable quantification of the assay was 0.18 pg/ml and the antibody pairs recognized Aβ multimers comprised of either synthetic standards, or endogenous oligomers isolated from confirmed human AD and healthy control brain. (jneurosci.org)
In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. (immpact-international.org)
Here, we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive, and robust targeted mass spectrometric method selected reaction monitoring, SRM. (nih.gov)

Additionally, for complex biological matrices, antibody selection can be challenging since protein targets secreted from cells into cell culture media or serum may be cleaved or modified, altering epitope recognition-hence the benefit of using protease cocktail inhibitors. (genengnews.com)
The concentration of TSH in circulation is expressed as milli-international units of biological activity per liter of serum (mIU/L). This way of expressing the concentration originates from the way in which the TSH (and other hormones) was initially evaluated. (medscape.com)

ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)

Full validation of the above assays, including intra-assay/inter-assay variability, spike recovery, dilution linearity, and limit of detection, is not usually reported. (jneurosci.org)
Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
The dynamic range of an assay defines both the lower and upper limits of detection. (genengnews.com)
BioAssay Systems' DPEC-100 kit provides a rapid and convenient assay for pectinase enzyme activity, in which pectin the substrate forms a turbid complex with the detection reagent. (bioassaysys.com)
[ 2 ] Although not completely reliable, an immunoassay and a mouse biologic assay are available for detection of ciguatoxin in affected fish. (medscape.com)

Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask. (abcam.com)
In the total antioxidant capacity assay protocol, the Cu2 + ion is converted to Cu + by both small molecule and protein antioxidants. (abcam.com)
This product is manufactured by BioVision, an Abcam company and was previously called K274 Total Antioxidant Capacity (TAC) Colorimetric Assay Kit. (abcam.com)
The Antioxidants Reagent Assays and the kits are used to assess the total antioxidant capacity of biological fluids, cells, and tissue. (pharmiweb.com)

For quantitative determination of pectinase activity determination in biological samples. (bioassaysys.com)
Hsa_circ_001242 expression levels in 40 paired OSCC tissues and four OSCC cell lines were selected using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). (hindawi.com)

Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases. (abcam.com)
We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)

Until now the standard procedure to validate antibodies for IP couples the IP assay to immunoblotting (IP/IB), a procedure that is challenging to scale to high-throughput analysis [ 10 ]. (biomedcentral.com)

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you. (bioassaysys.com)

The collagen content of the BTC and cellular proliferation of fibroblasts were assessed using colorimetric assays. (researchgate.net)

Here she developed nanoparticle sensors for biological assays. (wikipedia.org)

LncRNA CASP5 have been found to be up-regulated in GBM tissues compared with normal tissues in a microarray-based lncRNA profiling study. (cancerindex.org)
Forty oral cancer tissue samples and paired adjacent normal tissues were collected from the Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Peking University (Shenzhen, China), from December 2016 to May 2017. (hindawi.com)

Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)
We subjected diseased primary human lung fibroblasts to an advanced three-dimensional phenotypic high-content assay and screened a repurposing drug library of small molecules for inhibiting ECM deposition. (lu.se)

Here, we show that persistent DNA damage accumulation in tissue-infiltrating macrophages carrying an ERCC1-XPF DNA repair defect ( Er1 F/− ) triggers Golgi dispersal, dilation of endoplasmic reticulum, autophagy and exosome biogenesis leading to the secretion of extracellular vesicles (EVs) in vivo and ex vivo. (nature.com)
The aim of this study was to evaluate the sealing capability and tissue interaction of mussel-mimetic tissue adhesive (mussel glue) in comparison to fibrin glue on punctured fetal membranes in vivo. (researchgate.net)
Antifibrotic activity of N23Ps was verified by proteomics in a human ex vivo tissue fibrosis disease model, suppressing profibrotic markers SERPINE1 and CXCL8. (lu.se)

Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress. (abcam.com)

We sought to develop a robust assay to quantify oligomers from CSF. (jneurosci.org)
We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synonymous mutations, and post-translational modifications. (nih.gov)

Biochips market 2019-2024 Analyzed by Type - DNA, Protein, Lab-On-Chip and Other (Cellular chips, Tissue chips, Drug of Abuse Chips. (medgadget.com)
Further, we use these materials to develop novel techniques to characterize and screen cell-material interactions, rapidly detect biological molecules through controlled surface chemistries, and develop models to study cellular pathology. (colorado.edu)
The absence of antioxidants in the body and excess of Free radicals and reactive oxygen species can cause tissue and cellular injuries leading to further damaging of DNA, proteins, carbohydrates and lipids. (pharmiweb.com)
The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)
The whole system can be kept in an incubator, allowing long-term cellular assays to be performed. (moleculardevices.com)

The flowchip is composed of sample wells, which contain organoids, connected to additional multiple wells that can hold various assay reagents. (moleculardevices.com)

Applicants are required to have hands-on experience in organic/polymer synthesis and characterization of mechanical/biological properties of synthetic materials. (icmrs.net)

regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. (benthamscience.com)
Through the biological assays we intend to determine the anti-oxidant potential of the oligosaccharides produced in the tissues of organs (intestine, pancreas liver and spleen), as well as the growth of probiotic bacteria of the genus Lactobacillus and Bifidobacterium in the cecal lumial material. (fapesp.br)
There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translational research. (moleculardevices.com)
IVDs for clinical decisions on diagnosis and treatment, as does screening of blood/blood products for transfusion and human organs/tissues for transplantation. (who.int)

The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not easily scaled for high-throughput analysis. (biomedcentral.com)
Procedure: Assay takes 30 min. (immpact-international.org)
In this study the ACC hydrogels were prepared through freeze drying procedure and their angiogenic potential was studied by chorioallantoic membrane assay (CAM assay). (lancs.ac.uk)
Another recent project involved Suzanne's collaboration with fellow researchers on another CDC campus, working on adapting an assay or investigative (analytic) procedure on botulinum neurotoxin human exposure for state public health labs to follow. (cdc.gov)

The immunoprecipitation assay (IP) is an important tool used in academic and industrial research labs for applications such as targeted protein purification, protein concentration, analysis of protein-protein interactions, identification/analysis of protein complexes and analysis of protein/DNA interactions using chromatin IP (ChIP). (biomedcentral.com)
The ability to reliably and reproducibly measure any protein of the human proteome in any tissue or cell type would be transformative for understanding systems-level properties as well as specific pathways in physiology and disease. (nih.gov)

Collects, label, and distributesbiological specimens and tissues from. (employdiversity.com)

The biological indicators in bioassay are the reactions of living organisms or tissues. (pharmacologycorner.com)

Underlying biological processes involve induced massive deposition of extracellular matrix (ECM) by aberrant fibroblasts. (lu.se)

A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)
Wash-based assays can be used to circumvent interference caused by highly complex sample types, as molecules within the matrix that contribute to interference are washed away. (genengnews.com)
Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)

Collectively, these factors are known to influence data quality, biological relevance, and therapeutic predictability, which will ultimately impact the success of entire preclinical drug development efforts. (genengnews.com)
Additionally, primary cell and tissue sources for preclinical development might be quite difficult to obtain. (healthworkscollective.com)

Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)

A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)

The Laboratory Response Network at CDC will distribute the completed assay to State labs across the country. (cdc.gov)

Using mice with an engineered ERCC1-XPF defect in tissue-infiltrating macrophages, we provide evidence for a fundamental mechanism by which irreparable DNA damage triggers an exosome-based, metabolic reprogramming that leads to chronic inflammation and tissue pathology in NER progeroid syndromes and likely also during aging. (nature.com)
The assignment comprises transcriptomic (bulk and sc-mRNA-seq), epigenomic (ATACseq, CUT&RUN), proteomic (Olink assays), and compositional, metabolic and immunophenotypic profiling (high dimensional cytometry). (lu.se)

A major focus of research in the Anseth group is the development of biomaterial scaffolds with highly-controlled architectures and chemistries for three-dimensional cell culture, tissue regeneration, and biological arrays and/or assays. (colorado.edu)
Exosomes are being utilized in a variety of biomedical applications, including targeted drug delivery, gene therapy, diagnosis, and tissue regeneration. (selectbiosciences.com)

Low interference in biological samples. (immpact-international.org)
Assay technologies may not always be compatible with particular matrices, which can cause interference. (genengnews.com)
Assay technologies relying on excitation or emission at these wavelengths will be prone to interference. (genengnews.com)

Bioprocess considerations covering the cell isolation step starting from different tissue sources (i.e., stem/progenitor cells vs. more committed precursors), as well as the need for robust and cost-effective protocols featuring easy scalability envisioning clinical applications, which are approached herein. (mdpi.com)

In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions. (cshl.edu)

The optical density of the calibrator matches the optical density of wells assayed with 100 U/L pectinase under the standard assay conditions. (bioassaysys.com)

In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. (cshl.edu)

Botulinum toxins (BTX) are substances that are poisonous or destructive to nerve tissue and produced by rod-shaped bacteria known as Clostridium botulinum , and related species. (cdc.gov)

Mechanical forces play a vital role for many biological mechanisms, such as the sensation of infected cells by T-cells. (hu-berlin.de)

The diversity of proteoglycans and their high interaction with growth factors and their receptors provides structural basis for a multitude of biological functions. (premiumbeautynews.com)

Fig. 1b, c ) or the pancreas and the white adipose tissue (WAT) that are infiltrated with MAC1-possitve macrophages expressing YFP (Fig. 1d ). (nature.com)

Using a case study approach, the study applied the available literature to build the biological plausibility for 'tissue injury', a KE of regulatory relevance to MNs. (cdc.gov)

Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
Our goal is for each student to design a set of experiments focusing on their gene or biological interest. (cshl.edu)
The gene targeting experiments will be combined with other manipulations, such as tissue explants and transplants and live imaging to analyze the function of the genes. (cshl.edu)

This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)

This human SRMAtlas provides definitive coordinates that conclusively identify the respective peptide in biological samples. (nih.gov)

Using the assay, a significant 3- to 5-fold increase in Aβ oligomers in human AD CSF compared with comparably aged controls was demonstrated. (jneurosci.org)
He is a Professor at the Department of Orthopaedics and Traumatology at the University of Hong Kong (HKU), Affiliate Research PI at the Human Tissue and Organ Degeneration Center of the Shenzhen Institutes of Advanced Technology (SIAT) of the Chinese Academy of Sciences. (icmrs.net)
Currently all TSH assays are using WHO purified calibrators (second IRP 80/558), which have 7-5 pg purified human pituitary extract and are the equivalent of 37 mIU. (medscape.com)
The postdoc project aims to use systems-level methodologies to investigate the impact of biological sex on human immunity. (lu.se)

Cell therapy contract manufacturers such as Scorpion Biological Services make sure that the technical, regulatory, access, and licensing considerations are taken care of. (healthworkscollective.com)

The results of the analysis reveal the various endpoints, assays and specific biological markers used for assessing and reporting tissue injury. (cdc.gov)
Process and assay development programs at CDMOs can be tailored to meet the specific requirements of biological input materials. (healthworkscollective.com)
Chemists have successfully synthesized specific ciguatoxins, ensuring that a practical supply will be available for future biological applications. (medscape.com)
Each level has specific controls for containment of microbes, or microscopic organisms, and biological agents. (cdc.gov)

Hence, it is desirable to start with a biochemical assay followed by a cell-based assay to corroborate results in a physiological context (that is, to provide orthogonal validation). (genengnews.com)
When the absolute amounts of standard used in the assay are known, the results of the assay can be used to estimate the amount - in absolute units - of biologically active material contained in the unknown preparation. (pharmacologycorner.com)

Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)

Muscle tissue engineering aims to repair or regenerate defective muscle tissue lost by trauma or disease. (mdpi.com)
Mild creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) elevations, if present, reflect muscle tissue breakdown. (medscape.com)

Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs. (muni.cz)

This, in turn, establishes chronic inflammation and tissue pathology in mice with important ramifications for DNA repair-deficient, progeroid syndromes and aging. (nature.com)

Chitosan/collagen-based hydrogels were studied for their promising role in skin tissue engineering applications due to their unique biocompatibility and biodegradation properties. (lancs.ac.uk)

Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)

Our research spans the spectrum of fundamental studies to better understand the role of the biomaterial environment on cell function and the biology of tissue formation to targeted clinical applications in the design of in situ forming cell carriers that promote healing. (colorado.edu)
Conclusions: Biological augmentation of cuff repair in order to improve clinical outcomes is currently highly topical. (researchgate.net)

Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
Precious" samples, such as limited cells or tissue, impact the permissible volume for testing. (genengnews.com)
For example, T-cells (and other cells in our immune system) use mechanical forces to explore their environment, by pulling or pushing the other cells or tissue surfaces. (hu-berlin.de)

1994. Threshold limit values for chemical substances and physical agents and biological exposure indices (1994- 1995). (cdc.gov)
1994. Biological monitoring of exposure to organophosphorus insecticides by assay of urinary alkylphosphates: influence of protective measures during manual operations with treated plants. (cdc.gov)

Expertise and capability in functional genomics and DNA based assay design can be applied to numerous disciplines, research interests and diagnostic test development in the food or animal testing areas. (dcu.ie)

CCK-8 assay, flow cytometric analysis, western blotting, orthotopic GBM model as well as transwell assay were performed to investigate the biological role of CASP5. (cancerindex.org)
In this project the candidate will use chick embryos, patient tissue material, and omics data to investigate how hotspot mutations in susceptibility genes affect healthy embryogenesis, organ formation and initation of the endocrine tumor form paraganglioma. (lu.se)

SOR), hydrogen peroxide, and 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) radical activity by a model (most biological y active) of the anticancer plant was also evaluated. (who.int)

Anatomy3

Organisms2

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes12

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Vitro7

Cytotoxicity3

High-throughput assay1

Apoptosis1

Plate assay2

Quantification4

Serum2

ELISA2

Detection7

Total Antioxidant Capacity4

Quantitative2

Fluids2

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BioAssay Systems1

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Nanoparticle1

Normal tissues2

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Vivo3

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Cellular6

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Characterization1

Organs4

Procedure4

Protein2

Specimens1

Living organisms1

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Molecules3

Preclinical2

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Detect1

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Regeneration2

Interference3

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METHODS1

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Pancreas1

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Gene3

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