• Mass spectrometry coupled to liquid chromatography is a powerful tool for the analysis of complex plant derivatives such as GADF. (unboundmedicine.com)
  • We use a combination of MS techniques, namely liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the polyphenolic constituents of the soluble fraction of GADF. (unboundmedicine.com)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. (degruyter.com)
  • We describe here a new reversed-phase high-performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. (cas.cz)
  • The purposes of this study were to develop a sensitive, specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection of 25-hydroxyvitamin D(2) and D(3) and establish reference intervals for these analytes. (nih.gov)
  • It also has a toxicology business that uses liquid chromatography tandem mass spectrometry for quantitative urine drug testing. (genomeweb.com)
  • The most comprehensive metabolite coverage is typically accomplished by leveraging a non-targeted approach using high-performance liquid chromatography (HPLC) coupled with high-resolution accurate mass (HRAM) mass spectrometry, commonly referred to as HPLC-MS, or LC-MS. LC-MS is capable of routine detection of thousands of unique chemical compounds, providing broad coverage of biologically relevant metabolites. (metabolon.com)
  • The initial testing was performed mostly using enzyme-linked immunosorbent assay, but by liquid chromatography-tandem mass spectrometry (LC-MS-MS) as well. (erowid.org)
  • Urine samples from both patients were analysed using liquid-chromatography tandem mass spectrometry (LC-MS/MS) following glucuronidase digestion and solid-phase extraction. (erowid.org)
  • Application of an innovative high-throughput liquid chromatography-tandem mass spectrometry method for simultaneous analysis of 18 hazardous drugs to rule out accidental acute chemotherapy exposures in health care workers. (cdc.gov)
  • Methods: We examined the feasibility of alternate high-performance liquid chromatographic-tandem mass spectrometry methods to simultaneously detect eighteen chemotherapy analytes in plasma and urine. (cdc.gov)
  • Urine samples were extracted using solid phase extraction (SPE), pre-concentrated, and analyzed by high-performance liquid chromatography-tandem mass spectrometry using turbo-ionspray atmospheric pressure ionization. (cdc.gov)
  • Quantification of Folic acid (FA) in fortified wheat flour was carried out using a triple quadrupole liquid chromatography tandem mass spectrometry method. (eurekaselect.com)
  • A total of 11 unknown degradation products were characterized using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS/MS). Two major degradation products (DP2 and DP3) were isolated using preparative HPLC and their structures were confirmed by conducting 1H and 13C NMR experiments. (lookchem.com)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can be used to analyze urine specimens and identify a much wider variety of substances to which a person might be exposed ( 3 ). (cdc.gov)
  • Here a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous analyses of multiple steroid hormones in cetacean blubber is validated and applied to 10 stranded humpback whales (Megaptera novaeangliae). (nist.gov)
  • To examine changes in circulating proteins in response to verapamil treatment, the researchers used liquid chromatography-tandem mass spectrometry of blood serum samples from subjects diagnosed with Type 1 diabetes within three months of diagnosis and at one year of follow-up. (sciencedaily.com)
  • Therefore, we developed and validated a liquid chromatography-tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. (elsevierpure.com)
  • Sal-T was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Linear and quantile regression analyses were used to determine the age-specific 2.5th and 97.5th percentiles for the general population (1675 men and 2453 women) and the population with health exclusions (1145 men and 1276 women). (medscape.com)
  • The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
  • Electrospray ionization mass spectrometry has been used to investigate possible urinary metabolites of BSH. (aspetjournals.org)
  • 2000) describe a method for determining perchlorate in urine samples using electrospray ionization mass spectrometry. (cdc.gov)
  • Turbulent flow chromatography TFC-tandem mass spectrometry supporting in vitro/vivo studies of NCEs in high throughput fashion. (irbm.com)
  • 140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)
  • They can then be further fragmented and re-analyzed in tandem mass spectrometry, often with a quadrupole ion trap, but also possible with tandem time of flight. (wikipedia.org)
  • Analytes were quantified in negative selected ion monitoring mode using a single quadrupole mass spectrometer. (cas.cz)
  • The digested protein can be analyzed with different types of mass spectrometers such as ESI-TOF or MALDI-TOF. (wikipedia.org)
  • Product-ion traces at m/z 120.141, 176.055, and 295.074 were used for quantitation of FA, and traces at m/z 442.236 were used as parent mass quantified using positive heated electrospray ionization (HESI).The validated method showed linearity ranging from 1.25 to 640 ng/ml of FA and a correlation coefficient of 0.996. (eurekaselect.com)
  • While the standard MS/MS approaches provide 4000-5000 protein identifications within a couple of hours of instrumentation time, we advocate here that the higher number of identified proteins does not always translate into better quantitation quality of the proteome analysis. (sdu.dk)
  • The results obtained in this study demonstrated comparable quantitation efficiency of 5 min DirectMS1 with both TMT and DIA methods, yet the latter two utilized a 10-20-fold longer instrumentation time. (sdu.dk)
  • A set of tools for detecting differentially abundant peptides and proteins in shotgun mass spectrometry-based proteomic experiments with tandem mass tag (TMT) labeling. (bioconductor.org)
  • The use of a peptide-mass fingerprint is fairly widespread in proteomic research. (wikipedia.org)
  • In bio-informatics, a peptide-mass fingerprint or peptide-mass map is a mass spectrum of a mixture of peptides that comes from a digested protein being analyzed. (wikipedia.org)
  • The mass spectrum serves as a fingerprint in the sense that it is a pattern that can serve to identify the protein. (wikipedia.org)
  • The method for forming a peptide-mass fingerprint, developed in 1993, consists of isolating a protein, breaking it down into individual peptides, and determining the masses of the peptides through some form of mass spectrometry. (wikipedia.org)
  • Once formed, a peptide-mass fingerprint can be used to search in databases for related protein or even genomic sequences, making it a powerful tool for annotation of protein-coding genes. (wikipedia.org)
  • Before analyzing with mass spectrometry, a protein must be accurately isolated and digested. (wikipedia.org)
  • Mechanism-based P450 inhibitors can be characterized as displaying NADPH-, concentration-, and time-dependent quasi-irreversible or irreversible inactivation because of chemical modification of the heme and/or protein, and the inactivation rate is diminished in the presence of a competing substrate ( Silverman, 1998 ). (aspetjournals.org)
  • To gain insight into this mechanism, we used mass spectrometry to investigate changes in protein expression in hESCs cultured in hypoxia. (uwo.ca)
  • Electrospray tandem mass spectrometry was performed to compare global protein expression of hESCs from each oxygen condition. (uwo.ca)
  • Based on immunological and mass spectrometric analysis, SALSA was estimated to constitute up to 4-10% of the total protein amount in meconium, making it one of the most abundant proteins. (researchgate.net)
  • Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
  • The developed chromatographic method was extended to tandem mass spectrometry (QTOF-MS) for the characterization of the degradation products. (lookchem.com)
  • For glycopeptide characterization, mass spectrometry has arisen as the go-to methodology. (news-medical.net)
  • Title : Evaluation of an Isotope Dilution HPLC Tandem Mass Spectrometry Candidate Reference Measurement Procedure for Total 17-β Estradiol in Human Serum Personal Author(s) : Botelho, Julianne Cook;Ribera, Ashley;Cooper, Hans C.;Vesper, Hubert W. (cdc.gov)
  • Extraction and quantification of total folate using a microbiological assay (MA) with Lactobacillus casei as the assay organism and Enzyme Linked Immuno Sorbent Assay (ELISA) by competitive binding rapid ELISA kit was also performed. (eurekaselect.com)
  • The work presented here outlines an analytical platform that utilizes ambient ionization mass spectrometry and additional techniques (e.g., tandem mass spectrometry) to analyze trace residues from drug paraphernalia to quickly detect both drugs and cutting agents. (nist.gov)
  • The method was validated in terms of retention time stabilities, limits of detection, linearity, recovery, and analytical accuracy. (cas.cz)
  • The application of these results into a consistent acquisition method was managed through the use of a timed stepping of collision energies and transfer settings (Figure 1A and B). (news-medical.net)
  • Recently, we presented the DirectMS1 method of ultrafast proteome-wide analysis based on minute-long LC gradients and MS1-only mass spectra acquisition. (sdu.dk)
  • At that time, the best analytical method available had sensitivities of 400 g/L. Subsequently, it was improved to 100 g/L by Aerojet Corporation. (cdc.gov)
  • These correspond to a complex mixture of unidentified adducts, 4,5 in-source fragments, multimers, true molecular ions and a vast assortment of isotopes only differentiable by retention time. (metabolon.com)
  • Screening used a combination of tandem mass spectrometry, molecular technologies and biochemical analysis. (who.int)
  • The screening ciency the Delfia® time-resolved fluores- tests themselves but includes all of the protocol utilized a combination of MS/ cence application (Wallac Oy) was used elements essential for every neonate MS, molecular technologies and bio- to determine levels of phenylalanine to have access to a screening system chemical analysis. (who.int)
  • Rapid Commun Mass Spectrom 2012 Jul 15;26(13):1533-40. (cdc.gov)
  • Finally, most of the identities were unequivocally confirmed by accurate mass measurements on the TOF spectrometer. (unboundmedicine.com)
  • The fragmented ions are then separated by mass-to-charge ratio based on the time of flight (TOF) through the spectrometer. (wikipedia.org)
  • The output received from a mass spectrometer comes in the form of a peak list. (wikipedia.org)
  • Chromatographic retention times were under 7 minutes. (cdc.gov)
  • Tandem mass spectrometry was used to identify the intracellular metabolites (cytokinin monophosphorylated, diphosphorylated, and triphosphorylated nucleotides) in riboside-treated cells. (cas.cz)
  • Analytical platforms such as mass spectrometry and nuclear magnetic resonance (NMR) have emerged as the dominant platforms enabling the robust detection of numerous metabolites. (metabolon.com)
  • a final blood sample was collected for analysis at the time of the morning drug administration on day 7, but dogs were not treated that day. (avma.org)
  • Kinetic analysis of enzyme inactivation in hepatocytes was used to describe the effect of these time-dependent inhibitors and derive the inhibition parameters k inact and K I , which generally were in good agreement with the values derived using recombinant P450s and human liver microsomes (HLMs). (aspetjournals.org)
  • Tandem MS-MS analysis was performed in selective reaction monitoring mode (SRM). (eurekaselect.com)
  • As well as the instrumental arrangement, a suitable software solution is essential to enable glycopeptide identification and cut laborious processing and de novo analysis time. (news-medical.net)
  • To demonstrate proof-of-concept, samples collected from syringe service programs throughout the state of Maryland were analyzed by direct analysis in real time - mass spectrometry (DART-MS) to provide rapid, near complete chemical profiles (drugs, cutting agents, and other compounds of interest). (nist.gov)
  • Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. (lu.se)
  • After ionization of the analytes the ions were detected using electrospray ionization-tandem mass spectrometry. (uni-wuerzburg.de)
  • The retention times ranged from 4 min to 13 min for the analytical run. (cdc.gov)
  • precision of retention times. (lu.se)
  • Find answers to common questions about applying for magnet time, preparing for experiments and other practical user information. (nationalmaglab.org)
  • Because of this sensitivity, sample preparation is likely the most important step in forming a peptide-mass fingerprint. (wikipedia.org)
  • While it can be analyzed to some degree on its own, in forming a peptide-mass fingerprint, the peak list is run through a database search to find homologous peptide sequences. (wikipedia.org)
  • Despite the fact that the classification workflow in ProteinScape has an explicit parameter to address this issue, the peptide mass of low abundant N-glycopeptides can be miscalculated at times, which can result in no or incorrect identifications. (news-medical.net)
  • Until recently, classification of N-glycopeptide spectra in ProteinScape (detection of glycopeptide spectra and calculation of glycan and peptide moiety mass) has centered on glycan fragment distances and oxonium ion detection when using typical CID conditions on ion trap or qTof instruments (see application note LCMS-66)4. (news-medical.net)
  • Because the mass of individual peptides is measured in forming a fingerprint, mixtures of different proteins can yield unreliable results. (wikipedia.org)
  • The mass spectrometers were purchased by a grant from the Hayes Investment Fund of the Ohio Board of Regents. (aspetjournals.org)
  • We suggest that children with rapidly progressing acute encephalitis-like syndrome at the time of the litchi harvest have intoxication caused by hypoglycemic toxins, rather than viral encephalitis, as previously suspected. (cdc.gov)
  • Five of the eight mothers were symptomatic at the time of their child(ren)'s diagnosis, and all mothers and maternal grandmothers evaluated molecularly and biochemically had a homoplasmic pathogenic variant in MT-ATP6, low citrulline, elevated C3, and/or elevated C5-OH. (stanford.edu)
  • Identification was based upon comparison of the retention time and enhanced product ion scan with reference standards. (erowid.org)
  • Renaissance RX uses gold-standard real-time PCR to provide pharmacogenetic testing, according to its website. (genomeweb.com)
  • We present drug testing results from 10,349 nail samples collected from high-risk cases during a 3-year period of time. (erowid.org)
  • Engineering, Lund University in environmental samples are generally time consuming. (lu.se)
  • Mean + SD serum metformin concentrations over time following IV (A) and oral (B) administration of a bolus dose of metformin hydrochloride to 7 mixed-breed dogs (phase 1) in a crossover experiment (interval of 1 week between treatments). (avma.org)
  • Mean + SD serum metformin concentrations over time following twice-daily oral administration of metformin (phase 2) to the 7 dogs in Figure 1 . (avma.org)
  • At the time of the previous IARC evaluation of atrazine (Vol. 73, 1999), the most relevant epidemiologic studies consisted of two cohort studies of manufacturing workers, three population-based case-control studies of lymphatic and hematopoietic malignancies in agricultural areas of the U.S., and a population-based case-control study of ovarian cancer in a rice-growing area of Italy. (who.int)
  • Elution of 25(OH)D(2), 25(OH)D(3), and Delta(9)-THC-D(3) was achieved after 3.0 minutes (total run time, 6.0 minutes). (nih.gov)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • Timely data on seasonal patterns in child and adolescent conditions can guide optimal timing of prevention and intervention strategies. (cdc.gov)
  • Fifty-three proteins showed significantly altered relative abundance over time in response to verapamil. (sciencedaily.com)
  • In these settings, (0,2)X-ring fragmentation of the innermost N-acetylglucosamine of the chitobiose core (+83 Da), and a loss of the complete N-glycan moiety by cleavage of both the N-glycosidic bond and the side-chain amide group of the N-glycosylated asparagine, produces a characteristic peak doublet with a mass difference of 17 Da. (news-medical.net)
  • Tienilic acid selectively inhibited CYP2C9-dependent diclofenac 4′-hydroxylation activity, and erythromycin, troleandomycin, and fluoxetine inhibited CYP3A4-dependent midazolam 1′-hydroxylation in a time- and concentration-dependent manner. (aspetjournals.org)
  • Fluoxetine also inhibited CYP2C19-dependent S -mephenytoin 4′-hydroxylation in a time- and concentration-dependent manner in hepatocytes, HLMs, and recombinant CYP2C19 ( K I 0.4 μM and k inact 0.5 min -1 ). (aspetjournals.org)
  • Mean area under the concentration-time curve extrapolated to infinity, maximum concentration, half-life, and time to maximum concentration was 74.99 and 154.16 h·μg/mL, 37.3 and 51.5 μg/mL, 0.96 and 1.11 hours, and 1.28 and 1.65 hours, respectively, for the IV and IV + IM groups. (avma.org)
  • By contrast, approximately 25% of SIDS victims are younger than 2 months at the time of death, according to several epidemiologic studies of SIDS. (medscape.com)
  • A clinically compatible case in which a high index of suspicion (credible threat or patient history regarding location and time) exists for trichothecene mycotoxins exposure, or an epidemiologic link exists between this case and a laboratory-confirmed case. (cdc.gov)
  • time, two factors that are of primary importance in drug discovery. (irbm.com)
  • This work demonstrates that caution is warranted in the interpretation of enzyme induction studies with metabolically stable, weak time-dependent inhibitors, which may have dramatic inhibitory effects on P450 activity in this system. (aspetjournals.org)
  • AIMS: The aim of this study was to determine the effects of life-time tobacco, alcohol, and substance use on psychological and behavioral variables among 10th grade students in Istanbul/Turkey. (who.int)