Time coli plasmids. Medical search. Frequent questions

We show that multiple lineages of FosA-producing extended spectrum β-lactamase Escherichia coli have circulated in France since 2012, potentially reducing the efficacy of fosfomycin in treating infections with antimicrobial drug-resistant gram-negative bacilli. (cdc.gov)
This mechanism is particularly relevant because it is disseminative and frequently associated with ESBL-producing Escherichia coli . (cdc.gov)
Citations to Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli. (jci.org)
Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli. (jci.org)
Therefore, we studied the fate of Escherichia coli DNA following phagocytosis of E. coli by polymorphonuclear and mononuclear leukocytes. (jci.org)
The role of caspase-1, caspase-4 and NLRP3 in regulating the host cell response evoked by uropathogenic Escherichia coli. (oru.se)
Transcriptional alterations in bladder epithelial cells in response to infection with different morphological states of uropathogenic Escherichia coli. (oru.se)
Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells. (igem.org)
Our objective was to investigate the plasmid replicon-types involved in spread of ESBLs among Bulgarian Klebsiella pneumoniae and Escherichia coli. (who.int)
These primers were added to starting plasmid template and thermal cycled to produce mutant DNA plasmids, which were subsequently transformed into competent Escherichia coli DH5α.The plasmid DNA was purified from all the obtained colonies and the preparation containing a plasmid mix was analyzed by DNA sequencing. (conicet.gov.ar)
In Escherichia coli , besides the PEPCK reaction, there is another pathway to form PEP from C 4 -dicarboxylic acid which involves the NAD- and NADP-dependent malic enzymes (MAEA and MAEB) and phosphoenolpyruvate synthase (PPS). (asm.org)
Little is known about the genomic diversity of Escherichia coli in healthy children from sub-Saharan Africa, even though this is pertinent to understanding bacterial evolution and ecology and their role in infection. (peerj.com)
These were spread over 37 seven-allele sequence types and the E. coli phylogroups A, B1, B2, C, D, E, F and Escherichia cryptic clade I. Immigration events accounted for three-quarters of the diversity within our study population, while one-quarter of variants appeared to have arisen from within-host evolution. (peerj.com)
Eight isolates contained a large nonconjugative plasmid that could transform Escherichia coli. (ox.ac.uk)
These sequences are cloned into the bacterium Escherichia coli using USER technology, where they are amplified and then assembled into a single plasmid. (diwou.com)
We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. (bvsalud.org)
This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
Furthermore, production of intracellular membrane vesicles in Escherichia coli can be triggered by expression of Acholeplasma laidlawii lipid glycosyltransferases [ 5 ]. (biomedcentral.com)
Of these, 11 isolates (4 Escherichia coli , 5 Klebsiella pneumoniae and 2 Enterobacter cloacae ) produced ESBL of the CT-X-M-15 type. (who.int)
Parmi celles-ci, onze isolats (quatre Escherichia coli , cinq Klebsiella pneumoniae et deux Enterobacter cloacae ) ont produit des -lactamases à spectre élargi de type CTX-M-15. (who.int)
Escherichia coli ATCC 25922 conducted by PCR using a combina- oxyimino-cephalosporins and mono- was used as a control strain. (who.int)
Escherichia coli is a gram-negative rod that is found as a normal commensal in the GI tract, which can produce ocular infection including corneal ulcer and endophthalmitis, which can result in a devastating outcome. (medscape.com)
Ranjith K, Arunasri K, Reddy GS, Adicherla H, Sharma S, Shivaji S. Global gene expression in Escherichia coli , isolated from the diseased ocular surface of the human eye with a potential to form biofilm. (medscape.com)
RESUME Nous avons étudié la résistance aux antimicrobiens et les profils plasmidiques des isolats d' Escherichia coli uropathogénique chez des malades hospitalisés et des malades externes à l'Hôpital universitaire jordanien en 2000 et 2001. (who.int)
Why is Bacillus subtilis respiratory Complex II not assembled when produced in Escherichia coli? (lu.se)
The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)
What was believed to be a routine Escherichia coli urinary tract infection harbored a particular gene making it resistant to colistin , usually viewed as a last-resort antibiotic for the treatment of gram-negative infections. (medscape.com)
The experimental part focus on the model organisms, Bacillus subtilis , Escherichia coli and Streptomyces coelicolor . (lu.se)
To achieve enzyme-activatable cMOs, we focused on the Escherichia coli nitroreductase NfsB as the triggering catalyst. (cdc.gov)

Here we report the prevalence and mechanisms of fosfomycin resistance among clinical human E. coli strains isolated in Paris, France. (cdc.gov)
Sometimes particular E. coli strains are sub-optimal for plasmid extraction. (zymoresearch.com)
When possible, stick to tried and tested strains like E. coli DH5α which contain mutations to lack certain endonucleases and increase plasmid stability. (zymoresearch.com)
Thus, these modified E. coli strains are used as workhorses for molecular cloning and plasmid production. (zymoresearch.com)
The second method uses chloramphenicol, an antibiotic that halts protein synthesis and decouples it from plasmid replication, when culturing strains containing a plasmid with a relaxed origin of replication. (zymoresearch.com)
The antimicrobial activity of cellular extracts was determined against the Gram (+) and Gram (-) indicator strains (L. monocytogenes FBUNT and E. coli MC4100). (conicet.gov.ar)
The National Cancer Institute's (NCI) solution is a technology that consists of three specialized bacterial strains and seven plasmids, developed around a genetic system in E. coli that was harnessed into an enabling platform technology, allowing for highly efficient, rapid, and direct manipulation of larger DNA sequences (up to 100kb) than previously enabled by conventional molecular biology methods. (federallabs.org)
Whole genome sequencing (WGS) performed at CDC on isolates from 15 patients* confirmed that the recent isolates did not share common strains or plasmids with the 2012 outbreak. (cdc.gov)
The emergence and spread of ESBL-producing Enterobacteriaceae in intensive care units (ICU) is said to be due to clonal dissemination of a few epidemic strains as well as horizontal transmission of resistance gene-carrying plasmids among bacterial organisms (Wu et al . (scialert.net)
This resistance has spread to strains of E. coli and other gram-negative bacteria (Iroha et al . (scialert.net)
Resistant strains have existed for a long time, Snesrud said, but previously these were due to genetic mutations on the genome that could only be passed on to daughter cells. (genomeweb.com)
The mcr-1 on transferrable plasmids can instead be passed on to "other strains and other species that they come in contact with in the environment," he said. (genomeweb.com)
Yet, most of what we know about E. coli stems from the investigation of laboratory strains, which fail to capture the ecology and evolution of this key organism "in the wild" ( Hobman, Penn & Pallen, 2007 ). (peerj.com)

In this study, we determined the fitness effects of the major antibiotic resistance plasmid pOXA-48_K8 in wild-type, ecologically compatible enterobacterial isolates from the human gut microbiota. (nature.com)
Sixty-three isolates, with transferable beta-lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL-, RAPD-, and plasmid replicon-type. (who.int)
Among E. coli, 59% of the isolates were clonally related. (who.int)
Isolates of that cluster produced CTX-M-15, belonged to the O25b-ST131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. (who.int)
To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among Bulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX-M-15-producing E. coli and IncL/M plasmids among CTX-M-3 positive K. pneumoniae isolates. (who.int)
Among the seven recent isolates that underwent WGS, only two E. coli ST167 isolates appeared to be related. (cdc.gov)
The present study evaluated the antimicrobial effect of different combinations of gentamicin and floroquinolones (ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin) against extended spectrum beta lactamase (ESBL) positive E. coli isolates using checkerboard method. (scialert.net)
One hundred and four clinical isolates of E. coli obtained from urine (26), blood (24), stool (20), sputum (19) and semen (14) were investigated for ESBL production. (scialert.net)
This study was therefore designed to investigate the in vitro therapeutic outcome of combining an aminoglycoside (gentamicin) and some floroquinolones including ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin at different ratios against E. coli isolates expressing ESBL enzymes. (scialert.net)
One hundred and four clinical isolates of E. coli were collected from the intensive care unit of Eastern Nigeria Medical Centre Enugu. (scialert.net)
We isolated and whole-genome sequenced up to five colonies of faecal E. coli from 66 asymptomatic children aged three-to-five years in rural Gambia (n = 88 isolates from 21 positive stools). (peerj.com)
Several isolates encode putative virulence factors commonly found in Enteropathogenic and Enteroaggregative E. coli, and 53% of the isolates encode resistance to three or more classes of antimicrobials. (peerj.com)
Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase. (ox.ac.uk)
ABSTRACT We investigated antimicrobial resistance patterns and plasmid profiles of uropathogenic Escher- ichia coli isolates from inpatients and outpatients at Jordan University Hospital in 2000 and 2001. (who.int)
E. coli accounted for 32.4% and 37.4% of all isolates respectively. (who.int)
A large, transferable R-plasmid of 28 kb was found in most E. coli isolates (67%) that were resistant to at least ampicillin, cotrimoxazole and tetracycline. (who.int)
Eight to 10 colonies of tance to fluoroquinolones among uropatho- each pure culture of E. coli were taken and genic E. coli isolates [ 1-14 ]. (who.int)
We included 53 multidrug-resistant E. munity and hospitalized patients at a major coli isolates that were susceptible to nalid- teaching hospital in Jordan. (who.int)

The possible dissemination of this gene is worrisome because fosA3 is generally surrounded by the IS 26 insertion sequence on a composite transposon borne by the IncFII conjugative plasmid, which is known to be a dissemination vector of resistance genes worldwide. (cdc.gov)
Some of the most clinically relevant resistance genes, such those encoding carbapenemases (ß-lactamase enzymes able to degrade carbapenem antibiotics), are carried on conjugative plasmids that spread across high-risk bacterial clones 5 , 6 . (nature.com)
Carbapenemases, enzymes that confer resistance to the carbapenem class of antibiotics, are believed to contribute to increasing transmission and regional spread of CRE because the genes encoding these enzymes can reside on mobile plasmids and can be transferred among bacterial species. (cdc.gov)
AMR, through acquired genes located on transposons or conjugative plasmids, is the horizontal transmission of genes required for a given bacteria to withstand antibiotics. (mdpi.com)
The E. coli was found to contain the mcr-1 gene on a transmissible plasmid as well as genes conferring other types of antibiotic resistance. (genomeweb.com)
But if the mcr-1 gene is transferred and ends up on a resistance plasmid that contained genes encoding carbapenemases or 16s methylation , for example, it could be dire. (genomeweb.com)
The E. coli in question wound up having two plasmids with different complements of other antibiotic resistance genes, including extended-spectrum beta-lactamase genes, but Snesrud emphasized that it was not a pan-resistant strain. (genomeweb.com)
Thus, resident E. coli in these children may constitute reservoirs of virulence- and resistance-associated genes. (peerj.com)
In contrast to cell-specific promoters, heat shock promoters drive the expression of genes at arbitrary timing via heat shock [ 4 ]. (biomedcentral.com)
Therefore, for detailed analysis of multifunctional genes, such as the components of the Ras-MAPK pathway described below, simultaneous time-specific and cell-specific knockdown is necessary. (biomedcentral.com)
Antimicrobial susceptibility testing, molecular typing, characterization of ESBL-encoding genes and the genetic environment, conjugation experiments and plasmid analysis were carried out. (who.int)

In 2016, Portugal reported the first imported case of a travel-related infection in Europe with an E. coli strain co-expressing fosA3 and CTX-M-15 ( 11 ). (cdc.gov)
Qualified cell banks should be used and tested for identity, viability, strain, genotype/phenotype, and presence/copy number of the plasmid vector of interest, along with a description of its structural elements. (bioprocessintl.com)
These E. coli contaminations include one strain, E. coli O157:H7, which can be toxic to humans, because cattle typically hold this strain in their digestive system. (wikipedia.org)
If you are experiencing low yields for your plasmid prep, double check that the strain you're using is best for plasmid propagation. (zymoresearch.com)
While many guides will recommend 12-16 hour cultures, every E. coli strain is slightly different in its growth rate and final density. (zymoresearch.com)
In particular, the extract obtained from the strain SN72 was four times more active on both E. coli and L. monocytogenes. (conicet.gov.ar)
To begin the procedure, transform an Nsa1 expression plasmid into a suitable E.coli expression strain. (jove.com)
Automation of the protocol on an ASSIST PLUS pipetting robot with a VIAFLO 12 channel 1250 μl electronic pipette offers an easy and efficient way to extract and purify plasmid DNA with minimal hands-on time, guaranteeing perfect and reproducible liquid handling while protecting the user from repetitive strain injuries. (integra-biosciences.com)
Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
The resistance was detected in a strain of Esherichia coli collected from a woman in Pennsylvania with a urinary tract infection, according to a report published in Antimicrobial Agents and Chemotherapy last month. (genomeweb.com)
Although clinical microbiology typically relies on single-colony picks (which has the potential to underestimate species diversity and transmission events), within-host diversity of E. coli in the gut is crucial to our understanding of inter-strain competition and co-operation and also for accurate diagnosis and epidemiological analyses. (peerj.com)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

A conjugation test was done using our conjugation protocol to verify that Conjugation Testing Plasmid 1 was transmitted during conjugation. (igem.org)
Incorporating our fitness results into a simple population dynamics model revealed a new set of conditions for plasmid stability in bacterial communities, with plasmid persistence increasing with bacterial diversity and becoming less dependent on conjugation. (nature.com)
Plasmids are extra-chromosomal mobile genetic elements able to transfer between bacteria through conjugation 1 . (nature.com)
These multiresistant plasmids can be transferred by conjugation. (medscape.com)

BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. (biochain.com)
QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
Zhixing, Y and Nahon, J-L (1995) DNA gyrase improves DNA transformation of E.coli cells with large recombinant plasmids. (stackexchange.com)
Although a number of workers have contributed to the concepts underlying genetic engineering based on recombinant DNA, Professor Stanley N. Cohen and his colleagues were the first to construct, in 1973, a biologically functional hybrid plasmid and in the following year to achieve actual expression of a foreign gene implanted in E. coli by the recombinant DNA method. (wolffund.org.il)

Second, studies tend to analyse the fitness effects of a single plasmid in a single bacterium. (nature.com)

In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. (biochain.com)
Transformation Timer reimagines a time keeping device for a routine biotech laboratory technique. (laurasplan.com)
The motor is timed to rotate every 90 seconds, the time it takes in some lab protocols for an E. coli cell to take up a DNA plasmid during bacterial transformation. (laurasplan.com)
Size of the plasmid plays a large part in transformation, so intuitively it makes me think supercoiling the plasmid prior to transformation would improve the efficiency. (stackexchange.com)
I thought transformation was simply DNA diffusing through holes, but are parts of the plasmid required to bind/interact with the host? (stackexchange.com)
Here is a report that shows significant increase in transformation efficiency for large plasmids (transformation was by electroporation. (stackexchange.com)

Antimicrobial resistance occurs through plasmid-mediated determinants. (medscape.com)
Positive cultures were submitted pathogens with antimicrobial susceptibility for antibiotic susceptibility using the disk patterns that have changed over time [ 1-6 ]. (who.int)

This R-plasmid reservoir may contribute to the spread of multiple antibiotic resistance in our Region. (who.int)

The use of silica membrane-based DNA purification kits is a convenient way to prepare plasmid DNA samples for cloning or subsequent analysis, e.g. sequencing or restriction analysis. (integra-biosciences.com)
Then, the purified plasmid DNA was digested by restriction enzymes. (toyobo-global.com)
Plasmid DNA revealed highly related restriction fragments though plasmids appeared to have undergone some evolution over time. (ox.ac.uk)

However, it is a time-consuming step in genetic analysis. (integra-biosciences.com)
Here, we present a suite of tools for genetic manipulation in the tomato pathogen C. michiganensis including a markerless deletion system, an integrative plasmid, and an R package for identification of permissive sites for plasmid integration. (biorxiv.org)

Moreover, genomic results showed a link between pOXA-48_K8 fitness effects and bacterial phylogeny, helping to explain plasmid epidemiology. (nature.com)
The particular plasmid type that contains the resistance elements is important to the epidemiology. (genomeweb.com)

Generated plasmids have been used in clinical trials for gene therapy and vaccines as well as in viral vector production. (bioprocessintl.com)
Whether a vector is kept at low copy number, low culture density, or culture overgrowth, this guide will help you navigate your purification woes and determine the best way to boost your plasmid yields from E. coli cultures. (zymoresearch.com)
After digestion and Antarctic Phosphotase treatment of the vector, I ligated, transformed into TOP10 E. Coli cells and then plated on Chloramphenicol plates. (igem.org)
The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)

To further analyze the capacity of mononuclear leukocytes to degrade E. coli DNA, chromosomal and plasmid DNA was isolated from ingested bacteria and subjected to agarose gel-electrophoresis. (jci.org)
The increasing prevalence of antibiotic-resistant uropathogenic E. coli (UPEC) together with the recognition of UPEC as intracellular bacteria confront our current treatment regimens of urinary tract infections. (oru.se)
Endotoxins, also known as lipopolysaccharides or LPS, are cell-membrane components of Gram-negative bacteria such as E. coli and are released during the lysis step of plasmid purification. (qiagen.com)
A few months ago, a group of Chinese scientists found that many gram-negative bacteria ( Enterobacteriaceae ) harbor the plasmid gene mcr-1 . (medscape.com)
[ 2 ] A plasmid enables bacteria to spread easily to other bacteria and generate resistance to colistin. (medscape.com)
[ 4 ] So although just reported, colistin resistance has been developing for some time, perhaps silently, because such bacteria as E coli are rarely tested for colistin resistance. (medscape.com)
However, it is important because, having plasmid as a basis, these bacteria can spread to a whole host of organisms. (medscape.com)

There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells. (bvsalud.org)

Sometimes the simplest way for how to increase plasmid yields is to just input more raw material. (zymoresearch.com)
Chloramphenicol treatment can stop protein production but allow the E. coli to continue to "amplify" the plasmids, resulting in increased yields during plasmid purification 1 . (zymoresearch.com)
The alkaline lysis-based miniprep protocol typically yields 5-15 μg of plasmid DNA from 1.5ml overnight cultures. (integra-biosciences.com)

Characterization of the plasmids 1 and 2 by fluorescence experiments. (igem.org)
I mean, E. coli is used in experiments everywhere, right? (metafilter.com)

Plasmid DNA of E. coli carrying a gene coding for ampicillin resistance remained intact for a 2-h period after ingestion, and was still able to transform recipient E. coli cells after this period. (jci.org)
All the four parts were incorporated into an ampicillin resistant plasmid backbone. (igem.org)

Identification of best suitable parameters ranges for a desired time-delay device. (igem.org)
The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines. (qiagen.com)
Suitable for high throughput extraction of plasmid from E. coli cells. (toyobo-global.com)

40% glycerol and then stored at -20 °C for terns and plasmid profiles of uropathogenic further investigation. (who.int)

Or perhaps supercoiling reduces efficiency of subsequent plasmid preps/transcription (though wouldn't the host's natural enzymes supercoil anyway? (stackexchange.com)

One method for plasmid amplification uses an inhibitory amount of chloramphenicol (170 µg/ml) added to a culture, which is then incubated further until plasmid purification (typically the next day) 2 . (zymoresearch.com)
A variation of this method that reports higher plasmid yield uses lower amounts of chloramphenicol (10-20 µg/ml) added to exponentially growing cells that are subsequently incubated overnight prior to plasmid purification 3 . (zymoresearch.com)
Alternatively, another study demonstrated increased plasmid yield by growth in the presence of sub-inhibitory concentrations of chloramphenicol (3-5 µg/ml) from the time of culture inoculation until plasmid was harvested the next day 4 . (zymoresearch.com)

High-yield protocols and extra buffer volumes are provided with the QIAGEN Plasmid Plus Kits. (qiagen.com)

We developed permissR, an R package to identify permissive sites for chromosomal integration, which can be used in conjunction with pSelAct-Express, a non-replicating integrative plasmid that enables recombination into a permissive genomic location. (biorxiv.org)

Carefully flick the tube 4-5 times to mix cells and DNA. (neb.com)
UPRT fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in mammalian and E. coli cells. (invivogen.com)
This afternoon I made competant cells with the ibpAB-I13504 plasmid in them. (igem.org)
If expression in mammalian or insect cells is required - to obtain post-translational modifications, for example - the same construct can be used without the need for time-consuming subcloning procedures. (qiagen.com)
MagExtractor -Plasmid- provides a simple and reliable method for the rapid purification of plasmid DNA from E. coli cells utilizing magnetic silica beads. (toyobo-global.com)
MagExtractor -Plasmid- (Code No. NPK-301) extracts plasmids from E. coli cells, MagExtractor -Viral RNA- (Code No. NPK-401) is a kit for extracting viral RNA from serum or plasma specimens. (toyobo-global.com)
Plasmid DNA was purified from JM109 E. coli cells (from 1.2 mL cultured medium) carrying pUC18 by plasmid purification kits. (toyobo-global.com)
Using transmission electron microscopy, we detected membranous structures in the cytoplasm of E. coli cells expressing P9. (biomedcentral.com)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

We investigated the occurrence and molecular features of all fosfomycin-resistant E. coli isolated from hospitalized patients during a 12-month period (August 2014-July 2015). (cdc.gov)
To gain a handle on the factors that are propelling the problem of AMR, molecular and patient-level investigations are necessary to better elucidate the time-varying and heterogeneous role of antibiotic selection pressure on emergence and selection of AMR. (europa.eu)
QIAGEN Plasmid Plus Kits are intended for molecular biology applications. (qiagen.com)

Being plasmid-mediated is a concern especially as colistin use is the "canary in the coal mine" of gram-negative resistance. (medscape.com)

Cattle CAFOs can also be a source of E. coli contamination in the food supply due to the prevalence of manure in CAFOs. (wikipedia.org)
These results help to explain the high prevalence of plasmids in the greatly diverse natural microbial communities. (nature.com)

Automation of the pipetting steps of the miniprep workflow with the ASSIST PLUS pipetting robot allows for more hands-free time for the user and increased reproducibility. (integra-biosciences.com)
The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization. (biochain.com)

Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS ( K175031 ), key for the lock of medium RBS ( K175032 ), lock for weak RBS ( K175029 ) or key for the lock of weak RBS ( K175030 ). (igem.org)

However, when pDNA is used as a transfection material, multiple plasmid constructs often are required to produce an individual product coupled with an escalating demand for plasmid quantity as production scales increase. (bioprocessintl.com)

E. coli représentait 32,4 % et 37,4 % de tous les isolats. (who.int)
On a trouvé un grand plasmide R, de 28 kb, dans la plupart des isolats d' E. coli (67 %) qui étaient résistants à l'ampicilline, au cotrimoxazole et à la tétracycline tout au moins. (who.int)

MACHEREY-NAGEL's NucleoSpin 96 Plasmid kit and NucleoVac 96 Vacuum Manifold are a proven method for high throughput plasmid DNA purification. (integra-biosciences.com)
The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing hands-on time. (qiagen.com)

The plasmid mixture was used to obtain an expression library in E. coli BL21. (conicet.gov.ar)

During this time, the company has successfully executed more than 240 DNA programs with process development, manufacturing, and stability studies for more than 35 different customers globally. (bioprocessintl.com)

There are a couple ways to ensure high yield plasmid preparations from E. coli cultures by using antibiotics for selective pressure. (zymoresearch.com)

Corneal infection due to E coli produce indolent corneal ulcers with poor prognosis because most of these patients of have an underlying immunocompromised disorder or have abnormal corneal surface with compromised protective barrier. (medscape.com)

In real-time PCR (qPCR), reactions are characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. (thermofisher.com)
The availability of these fluorogenic probes enabled the development of a real-time method for detecting only specific amplification products. (thermofisher.com)

This plasmid is inserted into the yeast cell Saccharomyces cerevisieae genome where it produces the receptors. (diwou.com)
Figure 1: 'Transposition reaction from plasmid entry into the recipient cell to integration of the transposon into the genome. (igem.org)

The pharmacodynamic study modelled the relationships between antibiotic exposure and AMR emergence over time for various classes of agents. (europa.eu)

In particular, RNAi is a powerful tool that enables time- or cell-specific knockdown via heat shock-inducible RNAi or cell-specific RNAi. (biomedcentral.com)
To investigate the temporal- and cell-specific profiles of the functions of Ras, we developed a new RNAi method that enables simultaneous time- and cell-specific knockdown. (biomedcentral.com)

Although phagocytosis and killing by mononuclear leukocytes was less efficient than that by polymorphonuclear leukocytes, only mononuclear leukocytes were able to degrade E. coli PC2166 DNA. (jci.org)

Stress-Induced Accumulation of DcAOX1 and DcAOX2a Transcripts Coincides with Critical Time Point for Structural Biomass Prediction in Carrot Primary Cultures (Daucus carota L. (frontiersin.org)
Purification from cultures grown in highly enriched media such as Terrific Broth (TB) yield higher amounts of plasmid DNA per ml of culture than standard Luria-Bertani Broth (LB). (zymoresearch.com)

Those include an antibiotic-free plasmid maintenance system, operator repressor titration (ORT), and cell-lysis technologies ( 2 - 4 ). (bioprocessintl.com)
Add 1-5 µl containing 1 pg-100 ng of plasmid DNA to the cell mixture. (neb.com)
It's imperative to ensure the correct amount of antibiotic is present in your culture, otherwise the lack of selection pressure will cause your culture to start to dilute out the plasmid during cell division. (zymoresearch.com)
Typical plasmid yield from an E. coli cell line carrying a high-copy plasmid is approx. (toyobo-global.com)
We have established a new RNAi method that performs simultaneous time- and cell-specific knockdown and have applied this to reveal temporal profiles of the Ras-MAPK pathway in the control of exploratory behavior under poor environmental conditions. (biomedcentral.com)
The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)

Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)

The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)

To develop a real-time fluorescence assay for the LightCycler to detect and characterize ETEC. (cdc.gov)

What is Real-Time PCR (qPCR)? (thermofisher.com)
Initially, intercalator dyes were used to measure real-time PCR (qPCR) products. (thermofisher.com)

Partial confirmation of sequence of both plasmids. (igem.org)

In addition, pDNA sizes used for viral constructs are usually larger (≥11 kb) ( 1 ) and can contain base-pair sequences (such as inverted terminal repeats) that cause problems in their plasmid production. (bioprocessintl.com)

Escherichia29

Strains13

Isolates18

Genes11

Strain13

Conjugation4

Recombinant5

Single plasmid1

Transformation6

Antimicrobial2

Antibiotic resistance1

Restriction3

Genetic2

Epidemiology2

Vector4

Bacteria7

Mammalian cells2

Yields3

Experiments2

Ampicillin2

Suitable3

Uropathogenic1

Enzymes1

Chloramphenicol3

Protocols1

Recombination1

Cells9

Molecular3

Resistance1

Prevalence2

Miniprep2

Backbone1

Transfection1

Isolats2

Vacuum Manifold2

Expression1

Stability1

High yield1

Produce1

Amplification2

Genome2

Emergence1

Enables2

Degrade1

Cultures2

Cell6

Protein1

Operon1

Fluorescence1

QPCR2

Sequence1

Constructs1