• The crystal structures of bovine pancreatic ribonuclease A (RNAse A) soaked in the following organic solvents are presented: 50% dioxane, 50% dimethylformamide, 70% dimethylsulfoxide, 70% 1,6‐hexanediol, 70% isopropanol, 50% R,S,R‐bisfuran alcohol, 70% t ‐butanol, 50% trifluoroethanol, or 1.0 M trimethylamine‐N‐oxide. (ncsu.edu)
  • In this first study, we utilized immunoprecipitation of wild-type and mutant FUS in the presence and absence of RNase, followed by LC MS/MS. The identified proteins represent those that directly or indirectly interact with FUS, with relatively high affinity that can be pulled down with immunoprecipitation. (uky.edu)
  • Immunofluorescent staining of proteins interacting with mutant FUS were localized to cytoplasmic inclusions. (uky.edu)
  • According to Sidney Altman it makes sense that there are enzymes, such as RNase P, which include RNA components in addition to proteins. (blogspot.com)
  • Ribosomes are also ribonucleoproteins (RNPs), but they are much more complicated than RNase P. It seems unreasonable that ribosomes, with three RNAs and about 50 proteins, would exist without less complex RNPs having come into existence first and having persisted throughout evolution. (blogspot.com)
  • The RNASEH2B gene provides instructions for making one part (subunit) of a group of proteins called the RNase H2 complex. (medlineplus.gov)
  • We screened the Nebraska Transposon Mutant Library of 1920 single-gene inactivations in S. aureus strain JE2, for increased susceptibility to the anti-staphylococcal antimicrobials (ciprofloxacin, oxacillin, linezolid, fosfomycin, daptomycin, mupirocin, vancomycin, and gentamicin). (frontiersin.org)
  • The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells. (justia.com)
  • For the putative effector gene smut_5844, a signal peptide was required to induce non-host cell death, whereas ribonuclease catalytic active sites were required for smut_2965. (biomedcentral.com)
  • All three resistant mutants carried mutations in their polymerase gene. (springer.com)
  • The majority of the gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathway enrichment responses were for membrane-building and ploidy-related processes, consistent with the excessive floral organs and altered cell size observed in the mutant. (biomedcentral.com)
  • Integrated mRNA and miRNA profiling data provided comprehensive gene expression information on the wild-type and multi-tepal mutant at the transcriptional level that could facilitate our understanding of the molecular mechanisms of floral patterning of C. goeringii . (biomedcentral.com)
  • RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. (biomedcentral.com)
  • In dsRNA feeding assays with several triggers, both mutant strains were strongly, but not completely, resistant to RNAi targeting the muscle-specific gene and the essential gene (Fig. 2B). (mindunwindart.com)
  • The RNASEH2B gene mutations that cause Aicardi-Goutières syndrome likely result in a dysfunctional RNase H2 complex. (medlineplus.gov)
  • Individuals with a single replicate of an mutant PLCG2 gene, which encodes the alternative PLC-gamma Two that is lacking in your autoinhibitory C-terminal Src homology A couple of (cSH2) area, show PLC-gamma 2-associated antibody deficit as well as immune system dysregulation (PLAID). (prmtsignal.com)
  • To better understand the antiviral defenses relevant to COVID-19, we used interferon-stimulated gene (ISG) expression screening to reveal that OAS1, through RNase L, potently inhibits SARS-CoV-2. (cdc.gov)
  • Site-directed mutations have been made at each of the two cis proline residues, 93 and 114, and a double mutant has been made. (protabank.org)
  • The concept of mutation was coined by Hugo De Vries in 1901, whom worked with plants species of the genus Oenothera where he discovered some phenotypic hereditary characteristics that he coined as "mutations" and "mutants" to those individuals that have these phenotypic alterations. (intechopen.com)
  • The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
  • In order to remain functional, resistant mutants of HIV-RT need to maintain their capability of incorporating nucleotide at a sufficient rate, as well as to discriminate against NRTIs or improve the excision rate of incorporated NRTIs [ 17 ]. (biomedcentral.com)
  • RNase-resistant, noninfectious virus-like particles containing exogenous RNA sequences (armored RNA) are good candidates as RNA controls and standards in RNA virus detection. (politicalespionage.com)
  • However, there are three studies where favipiravir-resistant mutants were obtained in vitro. (springer.com)
  • The interaction between WAGO-1 and RDE-12 was resistant to RNase A treatment, suggesting that the interaction is not bridged by RNA. (mindunwindart.com)
  • TDF is a non-selective RT inhibitor with demonstrated in vitro activity against both wild-type and mutant strains of HIV [ 4 - 6 ] and is also effective for treating chronic hepatitis B (CHB) [ 7 - 10 ]. (biomedcentral.com)
  • Comparison of the MSCS structures with inhibitor‐bound crystal structures of RNAse A reveals that the organic solvent molecules identify key interactions made by inhibitor molecules, highlighting ligand binding hot‐spots in the active site. (ncsu.edu)
  • To illustrate application of the method, simulations of the diffusional encounter of the extracellular ribonuclease, barnase, and its intracellular inhibitor, barstar, are described. (h-its.org)
  • Transformation efficiencies of Ras mutants at residue 61 range over three orders of magnitude, but the in vitro GTPase activity decreases 10-fold for all mutants. (ncsu.edu)
  • Genes controlling floral organ identity have been identified through the genetic analysis of homeotic mutants, leading to the ABCDE model, in which five classes of regulatory genes (A, B, C, D and E) work in a combinatorial manner to confer the organ identities of the four whorls [ 10 ]. (biomedcentral.com)
  • This complex is a ribonuclease, which means it is an enzyme that helps break down molecules containing RNA, a chemical cousin of DNA. (medlineplus.gov)
  • In particular, the RNase H2 complex normally helps break down molecules in which one strand of RNA is combined with one strand of DNA (RNA-DNA hybrids) when these molecules are no longer needed. (medlineplus.gov)
  • A number of these MLV RT mutants resulted in statistically significant decreases in fidelity (1.2 to 2.8-fold) whereas two mutants showed a statistically significant increase in fidelity (0.8-fold) relative to wild-type MLV RT. (wvu.edu)
  • MazF-Da, expressed using a cell-free protein synthesis system, is a six-base-recognition-specific ribonuclease that preferentially cleaves UACAAA sequences and weakly cleaves UACGAA and UACUAA sequences. (uibk.ac.at)
  • This mutant cleaved UACAAG sequences in addition to UACAAA sequences, but did not cleave UACGAA or UACUAA sequences, suggesting that Asn36 affects the loosening and narrowing of MazF-Da cleavage sequence recognition. (uibk.ac.at)
  • Several structural determinants of MLV RT were identified that were important for fidelity which included position V223 of the YVDD box, several residues of the dNTP-binding site, as well as residues residing in the RNase H domain of MLV RT. (wvu.edu)
  • Furthermore, these amino acid residues were observed to play critical roles in catalysis and viral replication, which was exhibited by the reductions in both viral titers as well as RT activities of these mutants. (wvu.edu)
  • The dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that bears two amino acid substitutions: G66E (glycine at position 66 replaced with glutamic acid) and G174A ( 14 , 35 , 38 ). (asm.org)
  • These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)
  • Multiple solvent crystal structures of ribonuclease A: An assessment of the method. (ncsu.edu)
  • This set of structures is compared with four sets of crystal structures of RNAse A from the protein data bank (PDB) and with the solution NMR structure to assess the validity of previously untested assumptions associated with MSCS analysis. (ncsu.edu)
  • Our crystal structures show that, in transforming mutants, switch II takes part in a network of hydrophobic interactions burying the nucleotide and precatalytic water molecule. (ncsu.edu)
  • To understand how protein synthesis is suppressed by mutant FUS mediated defects in RNA metabolism, we examined changes in a well conserved RNA turnover pathway namely: nonsense mediated decay (NMD). (uky.edu)
  • The bridging water molecule is absent in the transforming mutants, contributing to the burying of the nucleotide. (ncsu.edu)
  • RDE-12-specific antisera recognized a 100 kDa protein in that is absent or truncated in mutant animals (Fig 1B). (mindunwindart.com)
  • Interestingly, we found that WAGO-1 and RDE-12 do not co-immunoprecipitate (IP) order RepSox in mutants (Fig. 1D), where the majority of both endo- and exo-secondary siRNAs are absent [4, 11]. (mindunwindart.com)
  • These studies have shown that the molecular mechanism of TFV resistance is complex and involves reverse transcriptase, RNase and exonuclease activities of HIV-RT. (biomedcentral.com)
  • But when that simple version of RNase P was transplanted into yeast-replacing the more complex ribonucleoprotein version-everything worked fine. (blogspot.com)
  • The RNase H2 complex is also thought to be involved in DNA replication, error repair, and other cellular processes, including helping to prevent inappropriate immune system activation. (medlineplus.gov)
  • Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex. (medlineplus.gov)
  • In Trypanosoma brucei , the pathogen that causes headaches in evolutionists , RNase P consists merely of a single protein. (blogspot.com)
  • Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf. (ncsu.edu)
  • We propose a mechanism for intrinsic hydrolysis in Raf-bound Ras and elucidate structural features in the Q61 mutants that correlate with their potency to transform cells. (ncsu.edu)
  • Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)
  • We found that MazF-Da exhibited the highest activity at around 60°C. Analysis using mutants with a single mutation at an amino acid residue site that is well conserved across various MazF toxins showed that G18, E20, R25, and P26 were important for the ribonuclease activity of MazF-Da. (uibk.ac.at)
  • Our Mutant Proteinase K has higher specific activity and is more stable at room temperature compared with wild-type Proteinase K. It is a non-specific serine proteinase with broad substrates. (sbsgenetech.com)
  • RNase Activity: None detectable after incubation with RNA for 16 hours at 25°C. (sbsgenetech.com)
  • The application of this Mutant Proteinase K is similar to wild-type Proteinase K. But this mutant one has higher specific activity and is more stable at room temperature. (sbsgenetech.com)
  • Feng S, Cao Z. Is the role of human RNase H2 restricted to its enzyme activity? (medlineplus.gov)
  • In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
  • Error-prone DNA synthesis by retroviral reverse transcriptases (RTs) is a major contributor to variation in retroviral populations generating mutants that exhibit altered host tropisms, resistance to antiviral drugs, and the ability to escape the host's immune system. (wvu.edu)
  • The recognition sequence of the N36A mutant differed from that of the wild type. (uibk.ac.at)
  • Gentamicin efficacy was significantly improved, when treating larvae infected with the atpA mutant compared to wild type cells with gentamicin at a clinically relevant concentration. (frontiersin.org)
  • Protein translation assays using both N2A and ALS patient fibroblasts demonstrated suppression of protein biosynthesis in mutant FUS expressing cells. (uky.edu)
  • We found that NMD is hyperactivated in cells expressing mutant FUS, likely due to chronic suppression of protein translation shifting the pathways autoregulatory circuit to allow for hyperactivation. (uky.edu)
  • The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. (justia.com)
  • The present invention relates to methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells. (justia.com)
  • Mutant Proteinase K is active with SDS, urea, and EDTA and active between 15°C and 75°C. (sbsgenetech.com)
  • Perrino FW, Harvey S, Shaban NM, Hollis T. RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases. (medlineplus.gov)
  • This shows how experimental rates for a series of mutants and the dependence of rates on ionic strength can be reproduced well by Brownian dynamics simulations. (h-its.org)
  • Thermal unfolding experiments on four single mutants give delta Tm approximately equal to 10 degrees C and delta delta G0 (apparent) = 2-3 kcal/mol. (protabank.org)
  • The toxin MazF, belonging to the type II TA system, functions as a sequence-specific ribonuclease that recognizes 3 to 7 bases. (uibk.ac.at)
  • The 3-D structure of Bacillus cereus (569/H/9) beta-lactamase (EC3.5.2.6), which catalyses the hydrolysis of nearly all beta-lactams, hasbeen solved at 2.5 A resolution by the multiple isomorphous replacementmethod, with density modification and phase combination, from crystals ofthe native protein and of a specially designed mutant (T97C). (embl.de)
  • New research is showing that RNase P makes no sense in evolution's preconceived role. (blogspot.com)
  • VASA homolog RDE-12 is a WAGO-1 interactor As in other organisms, the upstream events in the RNAi response include processing of long dsRNA into siRNAs by the RNase III-related protein, Dicer, loading of siRNAs into an RNase H-related AGO protein, and scanning for target mRNAs by siRNA-mediated base-pairing that precisely positions AGO for target mRNA cleavage [5]. (mindunwindart.com)