• Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
  • As documented in a recent publication in Stem Cell Reports , their MOS can be used as patient avatars for studies involving direct viral infection, immune cell penetration and high-throughput therapeutic drug screening, something that is not obtainable with conventional patient-derived models. (technologynetworks.com)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
  • This clinical trial uses a laboratory test called a high throughput sensitivity assay in planning treatment for patients with relapsed or refractory acute myeloid leukemia. (clincosm.com)
  • To obtain results from a high throughput drug sensitivity assay within 10 days, procure drug within 14 days and initiate treatment within 21 days. (clincosm.com)
  • A patient receives a drug intervention based on the results of a high throughput sensitivity assay. (clincosm.com)
  • Patients receive 1 of 160 possible interventions based on high throughput drug sensitivity assay. (clincosm.com)
  • Whether treatment was administered in the time frame based on the high throughput drug screen. (clincosm.com)
  • Development of a Robust and Quantitative High Throughput Screening Method for Antibiotic Production in Bacterial Libraries. (unca.edu)
  • Within natural diffusion limit of vascularized tissue, tumor-derived MOS allowed sufficient penetration by therapeutic immune T-cells such as CAR-T, enabling a novel T cell potency assay to assess tumor killing by the engineered T-cells. (technologynetworks.com)
  • Such a model would be highly useful in investigating tumor responsiveness and in developing anti-tumor immune cell therapies. (technologynetworks.com)
  • MOS could be further integrated with deep-learning imaging analysis for rapid drug testing of small and heterogeneous clinical tumor biopsies. (technologynetworks.com)
  • Numerous studies have confirmed that in addition to interfering with the tumor inflammatory environment, anti-inflammatory agents can directly increase apoptosis and sensitivity to conventional therapies and decrease invasion and metastasis, making them useful candidates for cancer therapy. (ijbs.com)
  • Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. (broadinstitute.org)
  • Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
  • The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
  • Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
  • Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
  • Such a model would be very useful in the investigation of tumor response and in the development of anti-tumor immune cell therapies. (genericviabuy.com)
  • Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
  • Discuss the current challenges and key considerations to be taken when designing assays to identify new biomarkers in pan tumor applications. (hub-xchange.com)
  • Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
  • In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
  • The aim is to try to identify drugs that may be effective in killing leukemia cells for those patients who will not be cured with conventional chemotherapy. (clincosm.com)
  • The identification, optimization and synthesis of antineoplastic drugs from PnV molecules may lead to a new multitarget chemotherapy. (nature.com)
  • Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
  • Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. (broadinstitute.org)
  • A typical workflow for conventional bioprospecting and drug discovery. (microbialtec.com)
  • Medicinal plants used by traditional medical practitioners (TMP) to treat cancers are considered safe when used alone or combined with conventional therapy to ensure their effectiveness and eliminate the toxic effects of orthodox medicines. (who.int)
  • Discussion topics will cover Immune Biomarkers & Translational Research, Preclinical Research, Clinical Development, Cell Therapies and Next-Generation Therapies. (hub-xchange.com)
  • IPS applies machine learning and AI to help research teams evaluate potential new medicines and therapies, as well as identify novel biomarkers for predicting how patients may respond to treatment. (bms.com)
  • We identified kinases within the insulin signaling pathway and centrosome cycle as regulators of ependymoma cell proliferation, and their corresponding inhibitors as potential therapies. (nih.gov)
  • There, he has established a discovery chemistry group that focuses on developing first-in-class inhibitors for newly emerging biological targets, including resistant alleles of existing targets, as well as inhibitors of well-validated targets, such as Her3 and RAS, that have previously been considered recalcitrant to small molecule drug development. (stanford.edu)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • Corresponding cultures yielded a strain identified as M . haemophilum at the Netherlands National Institute for Public Health and the Environment (RIVM) by using the Inno-LiPA Mycobacteria v2 reverse line blot assay (Innogenetics, Ghent, Belgium). (cdc.gov)
  • Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)
  • Immunosuppressive agents encompassed a large number of drugs, mainly represented by anti-tumour necrosis factor (TNF)-α, anti-retroviral therapy (ARV), azathioprine (AZT), methotrexate (MTX), mycophenolate mofetil (MMF), non-steroidal anti-inflammatory drugs (NSAIDs), rituximab and steroids. (ersjournals.com)
  • All RA patients in SA are at increased risk of tuberculosis (TB), in particular patients using anti-tumour necrosis factor (TNF) biologic therapy. (samj.org.za)
  • Dr. Shen and his team continue to refine and improve upon the MOS technology and to spotlight its versatility, not only as a physiological model for screening potential personalized treatments, but for disease studies and a variety of other applications as well," said Ali Khademhosseini, Ph.D., TIBI's Director and CEO. (technologynetworks.com)
  • The goal is to use this laboratory assay to best match a drug to a patient's disease. (clincosm.com)
  • This assay best matches a drug to the patient's disease. (clincosm.com)
  • Dr. Shen and his team continue to refine and improve the MOS technology and demonstrate its versatility, not only as a physiological model for screening potential personalized treatments, but also for disease studies and a variety of applications of others," said Ali. (genericviabuy.com)
  • Single cell proteome approaches already have changed how we explore cellular mechanism in disease and during therapy. (frontiersin.org)
  • There is still no drug that alters the state of the disease. (helsinki.fi)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • Updated treatment recommendations for the therapy of rheumatoid arthritis (RA) in South Africa advocate early diagnosis, prompt initiation of disease-modifying anti-rheumatic drugs (DMARDs), and an intense treatment strategy where disease activity is assessed with a composite score such as the Simplified Disease Activity Index (SDAI). (samj.org.za)
  • Frequent assessments and escalation of therapy are necessary until low disease activity (LDA) (SDAI ≤11) or ideally remission (SDAI ≤3.3) is achieved. (samj.org.za)
  • and ( iii ) the emergence of biologic disease-modifying anti-rheumatic drugs (DMARDs). (samj.org.za)
  • With over 50 compounds in development and 40 disease areas being studied, diverse and unique career opportunities are available in areas from Small Molecule Drug Discovery to Nonclinical, and Translational Research. (bms.com)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • The promising preclinical results seen with our bimodal ASO, IM-T9P1, pave the way for the development of combination therapies targeting TLR9 and PD-L1 for the treatment of cancer. (izb-online.de)
  • The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
  • Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
  • FDA approved drugs not currently used to treat ependymoma were also identified that posses selective toxicity against ependymoma cells relative to normal NSCs both in vitro and in vivo, e.g., 5-fluorouracil. (nih.gov)
  • A recent in vitro screening of the Phoneutria nigriventer spider venom (PnV) antitumor effects by our group has shown that the venom significantly affected glioblastoma cell lines. (nature.com)
  • Moreover, the algorithm was able to distinguish cytotoxic vs. cytostatic drug effects and drug-resistant clones that will give rise to later relapse. (technologynetworks.com)
  • Since in classic chemotherapies it is observed a decrease in immune response, methotrexate (MTX) was used only to compare the PnV effects on innate immune cells with a highly immunosuppressive antineoplastic drug. (nature.com)
  • Studies in progress are selecting the venom molecules with antitumor and immunomodulatory effects and trying to better understand their mechanisms. (nature.com)
  • Additionally, the algorithm was able to distinguish between cytotoxic and cytostatic effects of drugs and drug-resistant clones that will cause subsequent relapse. (genericviabuy.com)
  • Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)
  • Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • Prior to Flagship Biosciences, he served in senior scientific roles in oncology drug development for OSI Pharmaceuticals and Pfizer. (hub-xchange.com)
  • Researchers anywhere can explore more than 6,000 drugs in the hub and search for possible new uses for them to jump-start new drug discovery. (broadinstitute.org)
  • Further more, one of the most recent approaches in drug discovery from microorganisms is cocultivation. (microbialtec.com)
  • If you are interested in microbial drug discovery services, please contact us for more details. (microbialtec.com)
  • We will also shortly discuss how leveraging single cell multi-omic approaches can be used to accelerate cellular biomarker discovery during clinical trials to predict response to therapy, follow responsive cell types, and define novel druggable target pathways. (frontiersin.org)
  • Nathanael Gray is the Krishnan-Shah Family Professor of Chemical and Systems Biology at Stanford, Co-Director of Cancer Drug Discovery Co-Leader of the Cancer Therapeutics Research Program, Member of Chem-H, and Program Leader for Small Molecule Drug Discovery for the Innovative Medicines Accelerator (IMA). (stanford.edu)
  • and discovery that sphingosine-1-phosphate receptor-1 (S1P1) is the pharmacologically relevant target of the immunosuppressant drug Fingomilod (FTY720) followed by the development of Siponimod (BAF312), which is currently used for the treatment of multiple sclerosis. (stanford.edu)
  • Brad Nelson has twenty five years of experience in leading rapid growth life science organizations, delivering new technology and applications for drug discovery, development and clinical markets. (hub-xchange.com)
  • Wolfe, A. L. Advances in antibiotic drug discovery: Reducing the barriers for antibiotic development" Future Medicinal Chemistry 2020 , 12(22) , 2067-2087. (unca.edu)
  • Their current application in oncology has revolutionized cancer treatment by improving the antitumor activity of several chemotherapeutic agents. (mdpi.com)
  • In the lab we systematically derivatize each targets core structure to improve both their antibacterial activity and their physical properties in order to make them a viable drug molecule. (unca.edu)
  • MOS also provide a unique platform for studying and developing immune cell therapy. (technologynetworks.com)
  • We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
  • Functional T-cell assays are exposed to bias at several levels (manufacturing, pre-analytical, analytical and immunological) [ 2 ] and, unfortunately, the lack of a gold standard precludes higher diagnostic accuracy. (ersjournals.com)
  • MOS also provides a unique platform for the study and development of immune cell therapy. (genericviabuy.com)
  • These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
  • Explore how novel tissue staining technologies can be applied to interrogate new drug targets and immune cell while preserving tissue architecture for spatial analysis. (hub-xchange.com)
  • A technique in which electric pulses, in kilovolts per centimeter and of microsecond-to-millisecond duration, cause a loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. (bvsalud.org)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
  • Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)
  • During his six year stay at GNF, Dr. Gray became the director of biological chemistry where he supervised a group of over fifty researchers integrating chemical, biological and pharmacological approaches towards the development of new experimental drugs. (stanford.edu)
  • Dr. Gray has also developed structure-based, generalized approaches for designing drugs to overcome one of the most common mechanisms of resistance observed against most kinase inhibitor drugs, mutation of the so-called 'gatekeeper' residue, which has been observed in resistance to drugs targeting BCR-ABL, c-KIT and PDGFR. (stanford.edu)
  • Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)
  • Despite screening, mycobacterial infections have been diagnosed after prolonged treatment in various patients ( 8 ). (cdc.gov)
  • In addition, rhodomycin A rendered gefitinib-resistant lung adenocarcinoma cells more sensitive to gefitinib treatment, implying a synergistic effect of the combination therapy. (oncotarget.com)
  • Against this background, the South African Rheumatism and Arthritis Association (SARAA) has proposed the development of an updated treatment strategy for the effective therapy of RA in South Africa (SA). (samj.org.za)
  • This symposium presents a remarkable opportunity to convene with other researchers, clinicians, and industry leaders to discuss the extraordinary progress and immense potential of new drug therapies and technologies in paving the way for a brighter future in cancer treatment. (nyas.org)
  • Dr. Shen's team was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (technologynetworks.com)
  • The team of Dr. Shen was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (genericviabuy.com)
  • The HIV/AIDS pandemic in the 1980s then gave a dramatic impulse to the technology of counting specific cells, since it became clear that the quantification of peripheral blood CD4+ T cells was crucial to follow the course of the infection, and eventually for monitoring response to therapy ( 2 ). (frontiersin.org)
  • MOS can be further integrated with deep learning image analysis for rapid drug testing of clinical biopsies of small and heterogeneous tumors. (genericviabuy.com)
  • Otherwise, patients receiving at least one immunosuppressive drug (especially steroids) are more likely to have an indeterminate result [ 1 , 4 , 7 , 8 ]. (ersjournals.com)
  • We made the hypothesis that the proportion of "bad" responders to mitogen among patients with negative QFT could be underestimated, especially during immunosuppressive drug intake. (ersjournals.com)
  • Biologic DMARD therapy should be considered for patients who have failed a 6-month trial of at least 3 synthetic DMARDs. (samj.org.za)
  • These recommendations provide practical suggestions for the screening and management of TB and other comorbidities, and offer an approach to monitoring of RA patients. (samj.org.za)
  • But in at least 50% of cases, the patients produce neutralizing antibodies, rendering therapy with these proteins ineffective. (genengnews.com)
  • At BMS, being a part of any one of our many Research departments means pursuing pathbreaking science in the foundational and leading-edge discoveries that begin in the lab, on to clinical trial, before becoming therapies that transform patients' lives. (bms.com)
  • The goal of discovering and developing molecules with these principles is for them to have an increased probability of success of becoming approved therapies that transform the lives of patients with serious diseases. (bms.com)
  • Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
  • Welcome to hubXchange's West Coast Immuno-Oncology Xchange 2021, bringing together executives from pharma and biotech to address and find solutions to the key issues faced in developing immuno-oncology therapies, through a series of highly-interactive roundtable discussions. (hub-xchange.com)
  • We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)
  • Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
  • The Drug Repurposing Hub is one of the most comprehensive and up-to-date biologically annotated collections of FDA-approved compounds in the world. (broadinstitute.org)
  • Our researchers are now extracting bacterial DNA from soil and marine habitats, cloning large fragments into, for example, bacterial artificial chromosomes, expressing in a host bacterium and screening the library for new compounds. (microbialtec.com)
  • Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma. (broadinstitute.org)
  • Further applications are possible in various areas of pharmacology, developments catalyzed by the use of simple enzyme assays for screening before testing in intact animals. (microbialtec.com)
  • Molecules from animal venoms are promising candidates for the development of new drugs. (nature.com)
  • Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • Microorganisms play an important role in the development of the chemistry of natural products and medical therapy. (microbialtec.com)
  • Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
  • In 2021, Dr. Gray joined Stanford University where he has joined the Stanford Cancer Institute, Chem-H and the Innovative Medicines Accelerator (IMA) to spur the development of prototype drugs. (stanford.edu)
  • Joseph Krueger serves as the scientific leader for Invicro's Advanced Pathology Services and is responsible for developing new biomarker applications to meet drug development needs. (hub-xchange.com)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • Amongst all the marine invertebrates, sponges represent the most described group with potential application in medicinal chemistry, representing a valid starting point for new drug leads due to their chemical defense mechanisms. (microbialtec.com)
  • We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)
  • These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
  • The most effective drugs are fenretinide and α-galactosylceramide (α-GalCer). (frontiersin.org)
  • Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)