• vivo
  • Compare the effectiveness, in terms of incidence of graft failure and incidence of greater than grade 1 acute graft-vs-host disease, of ex vivo manipulation of bone marrow cells comprising counterflow centrifugal elutriation for T-lymphocyte depletion followed by CD34-positive stem cell selection using CliniMACS vs Isolex 300i in patients with a hematologic malignancy undergoing allogeneic bone marrow transplantation from an HLA-identical sibling donor. (clinicaltrials.gov)
  • Patients in both arms then undergo ex vivo manipulated, T-lymphocyte-depleted, CD34-positive stem cell-selected, allogeneic bone marrow transplantation on day 0. (clinicaltrials.gov)
  • To figure out if IL-6 is a element implicated in the ex vivo bone marrow amplification noticed in the existence of PTH and Flt-3L, bone marrow cells had been cultured for 8 times with a single remedy of PTH, Flt-3L or PTH plus Flt-3L in the existence and absence of IL-6 at the time of plating. (c14-demethylase.com)
  • cells
  • Arm I: Allogeneic bone marrow cells are subjected to counterflow centrifugal elutriation (CCE) for T-lymphocyte depletion. (clinicaltrials.gov)
  • Complete bone marrow was isolated from wild-kind mice and seeded at 1.86105 cells/cm2 in the existence or absence of Flt-3L (a hundred ng/ml), PTH (10 nM), a mixture of both, or motor vehicle only, (A) Circulation cytometric analyses of Annexin V+ Propidium Iodide- (early apoptosis) cells executed on non-adherent cells. (c14-demethylase.com)
  • Bone marrow was isolated and flow cytometric analyses of Annexin V+ cells ended up done. (c14-demethylase.com)