• Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
  • Albumin binding affinity test and in vitro control-released assay were performed to screen these BF-30 derivatives and explore the mechanism of anti-tumour effects. (jcancer.org)
  • Surface plasmon resonance (SPR) measurements, plasma stability test and in vitro control-released assay all showed that LBF-14 exerted better druggability compared with the others. (jcancer.org)
  • Integration of a machine learning-derived signature of response with in vitro assessment of LP-184 efficacy facilitated the derivation of manageable yet robust biomarkers which can be used to predict drug sensitivity with high accuracy and clinical value. (biomedcentral.com)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. (ucdenver.edu)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • The MTT assay was used to examine the cytotoxic effects of fungal taxol against the human liver cancer cell line HepG2. (degruyter.com)
  • Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
  • The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • Molecular docking, Western Blot, and ELISA assays were conducted to investigate the targets of SFI in intervening in melanoma fatty acid metabolism. (bvsalud.org)
  • Apoptosis was confirmed by DNA fragmentation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay. (biomedcentral.com)
  • Western Blot and TUNEL results suggested that Nigella sativa seed extracts regulated the expression of pro- and anti- apoptotic genes, indicating its possible development as a potential therapeutic agent for cervical cancer upon further investigation. (biomedcentral.com)
  • 50 years of preclinical anticancer drug screening:empirical to target-driven approaches. (jscimedcentral.com)
  • The selected BF-30 derivative was further subjected to the preclinical efficacy study and chronic evaluation of anti-tumour effects melanoma-bearing model mice. (jcancer.org)
  • Open data on the gene expression of the NCI-60 cell lines, provides a unique opportunity to add another dimension to the preclinical development of such drugs by interrogating correlations with gene expression patterns. (biomedcentral.com)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • Culture B16-F10 melanoma cells for treatment with mangosteen ethanolic extract to determine cell viability by MTT and genotoxic effect by comet assay. (unifesp.br)
  • The leaf extract induced genotoxicity and apoptosis in B16-F10 cells, since the fragmentation of DNA in the comet assay. (unifesp.br)
  • Cell proliferation assays demonstrated that treatment with the more than 10,000 molecular weight fraction significantly inhibited viability in DLD‑1 cells. (spandidos-publications.com)
  • The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. (frontiersin.org)
  • Synthesis and biological evaluation of new 3(2)-pyridazinone derivatives as non-toxic anti-proliferative compounds against human colon carcinoma HCT116 cells. (amrita.edu)
  • Successive assays revealed that some compounds were endowed with a promising anti-proliferative effect against HCT116 cells, alone or stimulated by serotonin as a pro-inflammatory factor in order to mimick an inflamed model of cancer cell microenvironment. (amrita.edu)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • Metronidazole selectively radiosensitizes of metronidazole (in a range of therapeutic hypoxic cells without influencing the ra- concentrations) on PMN-generated free diation response of normal well-oxygenated radicals during phagocytosis activity in cells [ 5,7-9 ]. (who.int)
  • To evaluate the antitumor and antimicrobial activity of ethanolic extract of Garcinia mangostana L. (mangosteen) cultivated in southeastern Brazil.METHODS:Anatomical characterization and histochemical reactions were performed for structural identification and the presence of phytocompounds. (unifesp.br)
  • The ethanolic extract of mangosteen obtained in the resin, leaf and fruit showed antimicrobial activity against Staphylococcus aureus and Escherichia coli with a MIC at 0.1 mg/mL.CONCLUSION: In conclusion, we have demonstrated both antimicrobial and antitumor activity of ethanol extract of mangosteen emphasizing its therapeutic potential in infectious diseases and in cancer, such as melanoma. (unifesp.br)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • The present study aimed to investigate components other than phenolic compounds that may have antitumor effects against colorectal cancer (CRC). (spandidos-publications.com)
  • Therefore, AGEs in MSpf may be potential compounds for the development of antitumor drugs for the treatment of CRC with fewer adverse effects compared with existing antitumor drugs. (spandidos-publications.com)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • Compounds of interest include not only classical "drug-like" organic small molecules, but also peptides, proteins, and other bioactive chemical classes. (cancer.gov)
  • Screening of compounds obtained from plants for their pharmacological activity has resulted in the isolation of innumerable therapeutic agents. (scialert.net)
  • Ciccocioppo R designed the study, was in charge of the diagnostic and therapeutic management of the patients, analyzed and interpreted the data, wrote the manuscript, and obtained funding. (wjgnet.com)
  • In other hand, it promoted a hypotensive activity by significant reduction in arterial blood pressure without change heart rate, confirming the therapeutic use as antihypertensive for this plant. (researchgate.net)
  • Beyond showing how BMP signaling impacts DIPG, our study also identified the potent antitumor efficacy of Dacinostat for DIPG. (nature.com)
  • The efficacy of the drug was evaluated through in vivo imaging technology, HE staining, and immunofluorescence. (bvsalud.org)
  • On the basis of the above results 7 herbs were further screened in order to test their efficacy against targeted mycotoxins: Glycyrrhiza glabra, Tinospora cordifolia, Zingiber officinale, Curcuma longa, Centella asiatica, Silybum marianum, Withania somnifera. (europa.eu)
  • Consequently, plenty of strategies were developed to increase the therapeutic duration of BF-30 in vivo including enlarging the molecular size via PEGylation to prevent from the rapid renal filtration and enzymatic degradation [ 6 ]. (jcancer.org)
  • In vivo experiments demonstrate that these nanocapsules can target to tumor sites through fluorescence/magnetic resonance imaging and offer remarkable therapeutic results. (nature.com)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • Anticancer drugs: Keeping one step ahead. (jscimedcentral.com)
  • The contribution of synthetic organic chemistry to anticancer drug development. (jscimedcentral.com)
  • It is well established that the role of the tumor microenvironment (TME) in cancer progression and therapeutic resistance is crucial, but many of the underlying mechanisms are still being elucidated. (frontiersin.org)
  • Emerging evidence suggests that they provide a cellular mechanism for induction and emergence of drug resistance and contribute to increased invasive and metastatic potential. (frontiersin.org)
  • Diagnostic testing continues to improve, but multi-drug resistance to treatment is an increasing challenge. (cdc.gov)
  • Previous researches revealed that BF-30 is a phenylalanine- and lysine-rich polypeptide exhibiting broad spectrum and strong antibacterial properties without drug resistance. (jcancer.org)
  • Dr. Gray's research has had broad impact in the areas of kinase inhibitor design and in circumventing drug resistance. (stanford.edu)
  • Dr. Gray has also developed structure-based, generalized approaches for designing drugs to overcome one of the most common mechanisms of resistance observed against most kinase inhibitor drugs, mutation of the so-called 'gatekeeper' residue, which has been observed in resistance to drugs targeting BCR-ABL, c-KIT and PDGFR. (stanford.edu)
  • Recently, various innovative approaches have been explored to overcome the therapeutic resistance caused by hypoxia. (nature.com)
  • Additional screens for MMTV provirus entry sites in tumors revealed several other upregulated genes that are associated with other gene development pathways such as INT2, INT3, and INT4 [4,5,6]. (chemdiv.com)
  • Specifically, the use of leukocyte-derived membrane proteins to functionalize nanovesicles (leukosomes) can enable their long circulation and target the inflamed endothelium present in many inflammatory pathologies and tumors, making them a promising and versatile drug delivery system. (frontiersin.org)
  • Special advances are achieved in HER-positive tumors using targeted therapy with drugs which suppress the tumor cell growth factors (trastuzumab, herceptin). (cttjournal.com)
  • Smartly engineered nanocapsules can not only shrink and decompose into small-sized nanodrugs upon drug release but also can regulate the TME to overproduce reactive oxygen species for enhanced synergistic therapy in tumors. (nature.com)
  • Salmonella as an innovative therapeutic antitumor agent. (nih.gov)
  • 1985. Mutagenicity evaluation of phthalic acid esters and metabolites in Salmonella typhimurium cultures. (cdc.gov)
  • Guidebook on molecular modeling in drug design: Academic Press. (jscimedcentral.com)
  • Ooms F. Molecular modeling and computer aided drug design. (jscimedcentral.com)
  • Library has been assembled using in house structural biology insight, molecular stimulation-modeling, virtual screening of ChemDiv's novel chemistries and medicinal chemistry filtering/ranking of the resulting hits. (chemdiv.com)
  • The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. (cancer.gov)
  • The data on the prognostic and predictive role of the tumor molecular biomarkers, as well as on clinically used and cellular therapy approaches and developing targeted drugs are presented, and the prospects for the future research are outlined. (cttjournal.com)
  • Molecular defects are often represented by hereditary BRCA (Breast cancer gene) mutations leading to altered DNA repair, thus presuming higher efficiency of DNA-damaging agents, such as platinum drugs and poly-ADP-ribose polymerase (PARP) inhibitors. (cttjournal.com)
  • Drug Screening Assays, Antitumor" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
  • Updated treatment recommendations for the therapy of rheumatoid arthritis (RA) in South Africa advocate early diagnosis, prompt initiation of disease-modifying anti-rheumatic drugs (DMARDs), and an intense treatment strategy where disease activity is assessed with a composite score such as the Simplified Disease Activity Index (SDAI). (samj.org.za)
  • Rapid diagnosis of rheumatoid arthritis allows for earlier treatment with disease-modifying antirheumatic drugs, which is associated with better outcomes. (aafp.org)
  • Importantly, each of these pathways is known to respond effectively to different therapeutic agents, thus personalized diagnosis equates to personalized treatment. (nih.gov)
  • Hyperprolactinemia administration п‚· the first steps in cases of signs of hyperprolactinemia ought to be a crucial diagnosis, as discussed above, may involve dynamic testings, evaluation for macroprolactinemia and further laboratory tests to get rid of false constructive or negative outcomes. (ehd.org)
  • Billingsley ML. Druggable targets and targeted drugs: enhancing the development of new therapeutics. (jscimedcentral.com)
  • There, he has established a discovery chemistry group that focuses on developing first-in-class inhibitors for newly emerging biological targets, including resistant alleles of existing targets, as well as inhibitors of well-validated targets, such as Her3 and RAS, that have previously been considered recalcitrant to small molecule drug development. (stanford.edu)
  • Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications. (frontiersin.org)
  • Qi J, Wang W, Lu W, Chen W, Sun H, Shang A. Design and biological evaluation of novel BF-30 analogs for the treatment of malignant melanoma. (jcancer.org)
  • Many of the medical complications that arise are the direct consequence of the need to administer potent immunosuppressive agents, with their attendant risks of infection, malignancy and drug toxicity. (ersjournals.com)
  • They included protein medicines used in supportive care as well as those used as therapeutic agents. (springer.com)
  • The second section will address novel agents associated with liver injury in 2014 (referred to as "signals"), especially in terms of clinical, research and drug development implications. (wjgnet.com)
  • Additional disease-modifying antirheumatic drugs or biologic agents should be added if disease activity persists. (aafp.org)
  • Moreover, according to the requirements of US Food and Drug Administration (FDA), theranostic agents should be completely cleared from the body within a reasonable period of time. (nature.com)
  • Horizon Discovery is using the iQue Screener platform for immuno-oncology assays and services. (intellicyt.com)
  • Mechanism-based target identification and drug discovery in cancer research. (jscimedcentral.com)
  • Drug repositioning and pharmacophore identification in the discovery of hookworm MIF inhibitors. (jscimedcentral.com)
  • Organic chemistry in drug discovery. (jscimedcentral.com)
  • The role of the medicinal chemist in drug discovery--then and now. (jscimedcentral.com)
  • EFMC would like to remind his efforts, joint with Henk Timmerman and Han van de Waterbeemd, to launch the successful series of EFMC Short Course in Medicinal Chemistry in 2009 with a very successful course on "Improving Compound Quality: Physical Chemistry and DMPK Properties in Drug Discovery. (efmc.info)
  • Nathanael Gray is the Krishnan-Shah Family Professor of Chemical and Systems Biology at Stanford, Co-Director of Cancer Drug Discovery Co-Leader of the Cancer Therapeutics Research Program, Member of Chem-H, and Program Leader for Small Molecule Drug Discovery for the Innovative Medicines Accelerator (IMA). (stanford.edu)
  • and discovery that sphingosine-1-phosphate receptor-1 (S1P1) is the pharmacologically relevant target of the immunosuppressant drug Fingomilod (FTY720) followed by the development of Siponimod (BAF312), which is currently used for the treatment of multiple sclerosis. (stanford.edu)
  • In particular, the discovery of the new use of artemisinin derivatives as excellent anti-cancer drugs is also reviewed. (anti-agingfirewalls.com)
  • Cancer immunotherapy has greatly expanded the therapeutic options against cancer. (intellicyt.com)
  • Li X, Taratula O, Taratula O, Schumann C, Minko T. LHRH-Targeted Drug Delivery Systems for Cancer Therapy. (jscimedcentral.com)
  • Sawa M, Masai H. Drug design with Cdc7 kinase: a potential novel cancer therapy target. (jscimedcentral.com)
  • If the role of cancer heterocellular signaling via TNTs in the TME is proven to be crucial, then disrupting formation and maintenance of TNTs represents a novel therapeutic approach for ovarian and other similarly invasive peritoneal cancers. (frontiersin.org)
  • This symposium presents a remarkable opportunity to convene with other researchers, clinicians, and industry leaders to discuss the extraordinary progress and immense potential of new drug therapies and technologies in paving the way for a brighter future in cancer treatment. (nyas.org)
  • The increase in the incidence of cancer has contributed to the search for new therapeutic methods. (mdpi.com)
  • Hence, it seems reasonable that research and potential therapeutic strategies for these two subtypes of DIPG should be tailored specifically, focused on the now-known differential impacts of the H3.1K27M versus H3.3K27M mutations on cancer epigenomes. (nature.com)
  • Dutta S, Mahalanobish S, Saha S, Ghosh S and Sil PC: Natural products: An upcoming therapeutic approach to cancer. (spandidos-publications.com)
  • BACKGROUND: Addressing brain metastases in cancer presents substantial challenges due to limited therapeutic options and high mortality rates. (bvsalud.org)
  • From the paper: 'Carriers of pathogenic germline variants (PGV) in BRCA2 could soon be offered gastric cancer screening using gastroscopy. (cdc.gov)
  • In the longer term, some might even take part in lung cancer screening programs. (cdc.gov)
  • This article presents the roles of the Wnt/β-catenin pathway in cancer and current therapeutic strategies involving this pathway. (chemdiv.com)
  • In 2021, Dr. Gray joined Stanford University where he has joined the Stanford Cancer Institute, Chem-H and the Innovative Medicines Accelerator (IMA) to spur the development of prototype drugs. (stanford.edu)
  • Chemotherapeutic drugs target cancer-specific characteristics, such as alterations in DNA repair, which has complicated roles in oncogenesis and tumor progression. (biomedcentral.com)
  • The Part 2 post in this series is concerned with mainline anti-cancer drug interventions that simultaneously address multiple growth pathways, ones that are entering clinical practice. (anti-agingfirewalls.com)
  • Smart drug delivery systems (SDDSs) for cancer treatment are of considerable interest in the field of theranostics. (nature.com)
  • A study of new drug comparing with standard chemotherapy treatment (Docetaxel) in previously treated stage 4 lung cancer patients. (who.int)
  • These medicinal systems have identified more than 700 individual herbal extracts as well as several drug preparations which claim to treat and/or prevent several diseases including cancer [ 11 ]. (biomedcentral.com)
  • Studies have therefore proposed that blocking ACVR1 kinase activity may represent a useful therapeutic strategy for the DIPG subgroup carrying ACVR1 mutations 14 , 17 . (nature.com)
  • However, the chemical PEGylation may hamper the therapeutic effects of the BF-30 or other peptides because the increased steric hindrance prevents the binding of targeting molecules [ 6 ]. (jcancer.org)
  • In this study, lentiviral transfection of sh‑RNA was used to knock down the level of RRS1, to detect the effect of RRS1 on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis by COIP and dual‑luciferase reporter gene assays. (morulaivf.com)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • His achievements in understanding the role of physiochemical properties in drug activity, more precisely in the disposition of medicines ought to be particularly recognised. (efmc.info)
  • Using a dual luciferase assay, we assessed the effect of JMJD5 on the transcriptional activity of the c-Myc target gene. (morulaivf.com)
  • A number of formulation approaches have been employed to increase the solubility and oral absorption of some herbal extracts and products and subsequently to enhance their bioavailability and therapeutic activity. (europa.eu)
  • Understanding the tumorigenesis mechanisms of DIPG and identifying potential therapeutic strategies are main research foci in the DIPG field. (nature.com)
  • We obtained protein components from maple syrup by ammonium sulfate precipitation, and treatment with the protein fraction of maple syrup (MSpf) was found to exhibit a potential antitumor effect. (spandidos-publications.com)
  • Zhu K, Aykas DP and Rodriguez-Saona LE: Pattern recognition approach for the screening of potential adulteration of traditional and bourbon barrel-aged maple syrups by spectral fingerprinting and classical methods. (spandidos-publications.com)
  • Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • During his six year stay at GNF, Dr. Gray became the director of biological chemistry where he supervised a group of over fifty researchers integrating chemical, biological and pharmacological approaches towards the development of new experimental drugs. (stanford.edu)
  • Over recent years, treatment for metastatic melanoma has significantly evolved with the development and regulatory approval of several therapeutic classes of medications including both molecularly targeted and immunologically focused checkpoint inhibitor therapies. (bmj.com)
  • In a randomized trial of patients who were on stable disease-modifying antirheumatic drug (DMARD) regimens and in clinical remission for at least six months, 84% of patients who continued full DMARD treatment remained in remission after 12 months, compared with 61% who tapered DMARDs by 50%, and with 48% of those who stopped all DMARDs. (aafp.org)
  • To observe the effects of Asini Corii Colla, turtle carapace glue, and other drugs on the intestinal flora of nude mice with uterine fibroids model, so as to provide evidence for the clinical application of drugs. (hindawi.com)
  • While offering a vital therapeutic option for patients with advanced lung disease, LTx remains fraught with complications that threaten both the quality and duration of the recipient's life. (ersjournals.com)
  • Switches occur when patients receive medicines formally designated as biosimilars, but may also occur after manufacturing process changes have occurred, if the process changes lead to structural modifications or changes in the impurity profile of the biologic drug [ 5 ]. (springer.com)
  • These recommendations provide practical suggestions for the screening and management of TB and other comorbidities, and offer an approach to monitoring of RA patients. (samj.org.za)
  • Methotrexate should be the first-line disease-modifying antirheumatic drug in patients with rheumatoid arthritis unless there are contraindications. (aafp.org)
  • Patients should be screened for chronic infections, including latent tuberculosis, hepatitis B virus, and hepatitis C virus, before starting rheumatoid arthritis treatment. (aafp.org)
  • Patients who are in remission from rheumatoid arthritis for more than six months and on stable medication regimens are candidates for tapering or discontinuing disease-modifying antirheumatic drug or biologic treatment. (aafp.org)
  • We also present the data of our own research concerning evaluation of the prognostic role of cytokines and lymphocyte subpopulations in peripheral blood of the TNBC patients. (cttjournal.com)
  • assays indicated possible low cy to to xicity to the extract. (researchgate.net)
  • Green medicine is safe and more dependable than the costly synthetic drugs, many of which have adverse side effects ( Joseph and Raj, 2010a ). (scialert.net)
  • Inhibition of Angiotensin Converting Enzyme (ACE) is a modern therapeutic target in the treatment of hypertension. (scielo.br)
  • These nanocapsules are made of a PLGA-polymer matrix coated with Fe/FeO core-shell nanocrystals and co-loaded with chemotherapy drug and photothermal agent. (nature.com)
  • To the best of our knowledge, no reports are available for the screening of endophytic fungal taxol in this medicinal plant. (degruyter.com)
  • At therapeutic doses of metronidazole (4.98-24.86 μg/mL) significant inhibition of the production of reactive oxygen species was noted in both methods. (who.int)
  • However, there are still many challenges in developing TME-sensitive SDDSs to overcome the anaerobic problem and improve the therapeutic effect. (nature.com)
  • The samples were kept at 4 °C until metronidazole does not show any radiosen- the start of the assay. (who.int)
  • 2+ in intensity in archival tumor sample (or if not available fresh biopsy sample) involving at least 50 % of the tumor cell population as demonstrated prospectively by a centrally assessed ICH assay. (who.int)
  • The traditional procedure of TIL production is assayed for specific tumor recognition and usually takes 6-8 weeks. (biomedcentral.com)
  • The need to control all these variables often leads to time-consuming and very expensive screening studies that require the analysis of a single variable at a time. (frontiersin.org)
  • However, developing SDDSs with early diagnostic capability, enhanced drug delivery and efficient biodegradability still remains a scientific challenge. (nature.com)