• This study was taken up to evaluate the antigenotoxic potential of ginger in human peripheral blood lymphocytes. (scialert.net)
  • In vitro treatment of peripheral blood lymphocytes with TSO induced significant number of micronuclei in all samples. (scialert.net)
  • Cultured peripheral human lymphocytes were used as test system. (europa.eu)
  • Peripheral human lymphocytes are recommended in international guidelines (OECD, EC). (europa.eu)
  • mDC, derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) and stimulated with lipopolysaccharide (LPS) for the last 24 h, were phenotyped for the expression of the co-stimulatory molecules B7.1 and B7.2. (torvergata.it)
  • Synergy among thymocytes and peripheral lymph node cells in the in vitro generation of lymphocytes cytotoxic to alloantigens. (ox.ac.uk)
  • Chromosomal aberrations (hypo- and hyperdiploidy, deletions, breaks, and gaps) in peripheral lymphocytes and bone marrow cells are the predominant effects seen in humans. (cdc.gov)
  • The frequencies of chromosome aberrations in both vehicle and positive controls were within the expected range and verified the sensitivity of the assay and the efficacy of the S9-mix. (europa.eu)
  • Based on the results from the preliminary toxicity test, the doses selected for treatment of the initial mutagenesis assay ranged from 2.5 to 120 µg/mL and 20 to 160 µg/mL for the S9 non-activated and activated cultures, respectively. (europa.eu)
  • Based on the results of the preliminary toxicity test, the doses chosen for treatment of the extended treatment assay ranged from 0.31 to 15 µg/mL for non-activated cultures with a 24-hour exposure. (europa.eu)
  • Additionally, MarinBio specializes in in vitro cell-based assay testing services for clinical and pre-clinical studies, including method development, qualification, validation, transfer, lot-release testing, and potency testing according to guidelines from both FDA and International Council for Harmonization (ICH). (marinbio.com)
  • The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
  • It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs. (lookformedical.com)
  • Quality Assurance also includes testing for sterility, pH, osmolority, presence, or absence of Mycoplasma, endotoxin and presence of Human Leukocyte Antigen (HLA Ag) by Complement Dependent Cellular Cytotoxicity (CDCC) and flow cytometry. (labquip.net)
  • This material was tested and found to be non-reactive for hepatitis B Surface Antigen (HBsAg), Anti-HIV and Hepatitis C. (labquip.net)
  • In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
  • Hepatitis B is not a reliable antigen for testing immune competence because of the high frequency of nonresponders in the population, particularly in persons older than 40 years. (medscape.com)
  • Borrelia burgdorferi LFA-1 (Lymphocyte Function Antigen 1): Own body protein + Borrelia burgdorferi sensu stricto (shared epitope). (arminlabs.com)
  • Moreover, proliferative response of allogenic naive T cells cultured with mDC was impaired in the patients (13471 +/- 9917.2 versus 40976 +/- 24527.2 cpm, P = 0.04). (torvergata.it)
  • Diagnosis is by detecting lymphopenia, absence or a very low number of T cells, and impaired lymphocyte proliferative responses to mitogens. (msdmanuals.com)
  • Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)
  • Results from this experiment were confirmed by those of a second test with groups of female mice of the same strain fed dietary levels of captan ranging from 500 to 16 000 ppm for up to 213 days. (inchem.org)
  • a) Bone tissue marrow cells from BALB/c mice were cultured with GM-CSF and IL-4 to induce the creation of BMDCs. (sipurpashut.net)
  • Aqueous ginger extract was added at concentrations namely 0.1, 0.2 and 0.4 mg to test for antigenotoxicity. (scialert.net)
  • Two separate experiments were conducted, in the first five concentrations of test substance (50, 150, 500, 1500 and 5000 µg/plate) were assayed in triplicate against each tester strain, using the direct plate incorporation method. (europa.eu)
  • CTLL-2 cells were cultured with increasing concentrations of client drug for four days. (marinbio.com)
  • A study was conducted to determine the mutagenic potential of test substance, Coco TMAC (33% active in water), according to OECD 471 and EPA OPPTS 870.5265 Guidelines, in compliance with GLP. (europa.eu)
  • The mutagenic potential of the test substance, manganese dichloride, was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 471 and EU Method B.13/14. (europa.eu)
  • The potential of the test substance, manganese dichloride, to induce structural chromosomal aberrations in human lymphocyte cells in vitro was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD 473. (europa.eu)
  • The mutagenic potential of the test substance, manganese dichloride, was determined in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD 476. (europa.eu)
  • Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. (biomedcentral.com)
  • This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. (biomedcentral.com)
  • The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
  • Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. (biomedcentral.com)
  • This OPXV strain (Italy_09/17) was isolated on cell cultures and embryonated eggs. (cdc.gov)
  • During the study test strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and a tester strain of Escherichia coli (WP2uvrA) were exposed to the test substance both in the presence and the absence of metabolic activation. (europa.eu)
  • Alongside the production by B lymphocytes of virus-specific antibodies, the stimulation and multiplication (clonal expansion) of virus-specific T lymphocytes is a central feature of this immune response. (drroberthess.com)
  • The confirmation by this cell function test of prior Covid-19 immunization succeeds significantly more often than with the serological determination of antibodies, as SARS-CoV-2-specific antibodies are not always detectable even with a PCR test following an infection. (drroberthess.com)
  • Borrelia infections do not only show humoral immune responses by antibodies, but can activate T-lymphocytes at the same time. (arminlabs.com)
  • After a second centrifugation step, HBSS was removed and cells were resuspended in 5 mL culture medium and incubated for another 20 - 22 h (24 h fixation time). (europa.eu)
  • In six patients and six controls allogenic mixed leucocyte reaction (AMLR) was performed using mDC and cord blood-derived naive T cells at a DC/T naive ratio of 1 : 200. (torvergata.it)
  • During the study duplicate cultures of human lymphocyte cells, treated with test substance, were evaluated for chromosome aberrations over at least three dose levels. (europa.eu)
  • The test substance did not induce any toxicologically significant increases in the frequency of cells with aberrations in either of the 4(20)-hour exposure groups, in the absence or presence of a liver enzyme metabolising system, or following 24 hours continuous exposure. (europa.eu)
  • Periodically 'cleansed' of spontaneous mutants: Prior to dose-range finding and mutagenicity testing, the mouse lymphoma cells were grown for 1 day in R10-medium containing hypoxanthine, aminopterine and thymidine to reduce the amount of spontaneous mutants, followed by a recovery period of 2 days on R10-medium containing hypoxanthine and thymidine only. (europa.eu)
  • Cells were exposed to the test item in basic medium supplemented with 5%/10% (v/v) heat-inactivated horse serum (R5-medium) for 3/24 hour exposure. (europa.eu)
  • MarinBio's contract research and development service professionals are expert in designing customized or standardized bioassays and in the measurements of interactions of drugs (including small molecules, biologics, or device interactions) with cultured cells and primary cells. (marinbio.com)
  • In the course of the acute immune response, the T lymphocytes first differentiate into effector T cells, which are actively involved in the elimination of virus-infected cells (CD8 killer T cells) and in controlling the function of other immune cells (CD4 T helper cells). (drroberthess.com)
  • The ELISPOT method, on which our SARS-CoV-2 T-Cell immunity test is based, provides a sensitive and specific analysis of T cells in the blood samples submitted by our clients. (drroberthess.com)
  • This cell culture test is based on the release of messenger substances from the immune system (cytokines) by single cells after stimulation of lymphocytes with virus fragments (peptides). (drroberthess.com)
  • Our proposal focuses on the possibility to test agents selectively reducing the fraction of GPI-AP deficient cells and consequently improving the growth of GPI-AP normal cells. (aamds.org)
  • Our proposal is based on the possibility to pre-clinically test drugs improving the function of normal cells and decreasing the fractions of cells with PIGA mutations. (aamds.org)
  • Using a large screening of chemicals and natural compounds already approved by the U.S. Food and Drug Administration or tested in previous studies we identified one chemical substance called CGS- 15943 which has an effect in improving the proliferation of normal cells by decreasing the number of PNH cells. (aamds.org)
  • A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
  • Subsequent characterization of this virus has shown that it is not significantly related to any known animal retrovirus, is not an endogenous (genetically transmitted) virus of man, and so far has been associated only with fresh or cultured T -cells from patients with T -cell neoplasia. (science-connections.com)
  • 1975) have required the establishment of continuously growing cell lines from the disease of origin or cocultivation of these cells with previously established cell lines, the ability to grow malignant T -cells in long-term culture could facilitate the isolation of retroviruses from these diseased states. (science-connections.com)
  • Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. (nature.com)
  • It seems that these tests will be used in the future, apart from serological tests. (arminlabs.com)
  • Clinicians should not refer patients to CDC to find out where or how to get tested for COVID-19 or how to get COVID-19 test results. (cdc.gov)
  • The test substance was therefore considered to be non-clastogenic to human lymphocytes in vitro. (europa.eu)
  • In this study, we tested the effect of local administration of human MSC-Exo on established EAU in the same species. (nature.com)
  • In early tests, the biomechanical treatment removed more than 90 percent of toxins from infected human blood within a few hours. (discovermagazine.com)
  • Researchers then tested their device by cleaning 5 liters of human blood spiked with a variety of pathogens. (discovermagazine.com)
  • The next step for researchers is to test their device in pigs, after which human trials could begin within a couple years, Nature reports. (discovermagazine.com)
  • Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)
  • Toxicity in the cloned cultures was observed at doses of 10 and 15 µg/mL. (europa.eu)
  • Scientists tested their device by infecting rats with lethal doses of either Escherichia coli or Staphylococcus aureus and then cleaning their blood. (discovermagazine.com)
  • Based on the results of the three in vitro studies, the test substance is considered to have no genotoxicity potential. (europa.eu)
  • So far, we have been testing only one of the two immunity pillars, namely antibody production. (drroberthess.com)
  • Under the study conditions, the test substance is not considered to be mutagenic in the presence and absence of exogenous metabolic activation. (europa.eu)
  • No toxicologically significant increases in the frequency of revertant colonies were recorded for and of the bacterial strains, with any dose of the test substance, with or without metabolic activation. (europa.eu)
  • Three treatment conditions were used for the study, as follows: firstly, cultures were exposed for 4 hours with a 20 hour expression time, both in the presence and absence of metabolic activation (S9 mix), secondly cultures were continuously exposed for 24 hours in the absence of metabolic activation. (europa.eu)
  • Precipitate of the test substance was observed at and above 10 µg/mL in the 4-hour exposure groups in the absence of metabolic activation and at and above 20 µg/mL in the 4-hour exposure group in the presence of metabolic activation. (europa.eu)
  • The test substance was found to cause no visible reduction in growth of the bacterial background lawn at any dose and was therefore tested up to the maximum dose level of 5000 µg/plate A particulate precipitate was at 1500 µg/plate and above. (europa.eu)
  • Therefore, under the conditions of the study the test substance was concluded to be non-mutagenic. (europa.eu)
  • Laboratory tests showed leukocytosis (20.79 × 10 9 /L), moderate anemia (69 g/L), increased percent of neutrophils (91.6%), increased C-reactive protein (CRP) (80.90 mg/L), procalcitonin (PCT) (5.87 ng/ml) and erythrocyte sedimentation rate (ESR) (106.0 mm/h) level, and mild hypoalbuminemia (31.0 g/L). Stool routine revealed Leukocyte ++++/HP, pus cell ++++/HP, stool blood was positive. (frontiersin.org)
  • 0.5 mL of whole blood in a total volume of 5 mL medium was cultured in the presence of 0.16 mM Trans Stilbene Oxide (TSO) for the induction of micronuclei. (scialert.net)
  • This supplementary CE-certified blood test facilitates the cellular immunological detection of a SARS-CoV-2 infection. (drroberthess.com)
  • When those nanobeads are mixed with infected blood they adhere to pathogens, and then, as the blood passes through channels inside the device, magnets pull out the beads with pathogens attached. (discovermagazine.com)
  • The blood culture was also positive for Y. enterocolitica in 1 case. (who.int)
  • It works by testing specific cellular immunity after a SARS-CoV-2 infection, as well as detecting immune reactivity against endemic coronaviruses, also referred to as background immunity. (drroberthess.com)
  • The fluorescence-based version of the ELISPOT method used for this test allows for the simultaneous detection of two different cytokines - interferon-y (IFN-y) and interleukin-2 (IL-2). (drroberthess.com)
  • LTT and EliSpot are a few of these tests. (arminlabs.com)
  • We also measured IL-10 and IL-12 concentration in the supernatant of DC cultures. (torvergata.it)
  • Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)
  • PHS supports Mixed Lymphocyte Culture (MLC) and Mitogen (PHA) Response. (labquip.net)
  • To determine whether TJ-M2010-2 affected DCs maturation and following T-cell proliferation, we examined the inhibitory ramifications of TJ-M2010-2 on lipopolysaccharides (LPS)-induced DC maturation and T-cell proliferation in combined lymphocyte response (MLR) program. (sipurpashut.net)
  • WHO is not responsible, and does not accept any liability, for the testing of pesticides for compliance with the specifications, nor for any methods recommended and/or used for testing compliance. (who.int)
  • We are experts in the art and science of cell culture. (marinbio.com)
  • Each unit is tested and found negative for: HBsAg, HCV, HIV-1, HIV-2, HIV-1Ag or HIV 1-NAT, ALT, and syphilis by FDA-Approved Methods. (labquip.net)
  • Recommended test system in international guidelines (e.g. (europa.eu)
  • All of the positive control substances induced marked increases in the frequency of revertant colonies, confirming the activity of the S9-mix and the sensitivity of the bacterial strains. (europa.eu)
  • However, the diagnosis can be made upon the onset of ataxia and before the appearance of telangiectasia if confirmed by laboratory tests. (shengsci.com)
  • A dermatologist can document and monitor a patient's cicatricial alopecia using these guidelines, and with the pull test. (wikipedia.org)
  • Furthermore, the focus of TJ-M2010-2 found in this research did not straight impact the cell viability of DCs and lymphocytes (find Supplementary Fig. S2). (sipurpashut.net)
  • In the second experiment the test substances and vehicle control were dosed using a pre-incubation method. (europa.eu)
  • Thus, in the oral cavity B lymphocytes contribute to control commensal C. albicans carriage by secreting IgA at foci of colonization thereby preventing fungal dysbiosis. (frontiersin.org)
  • in stools by cold-enrichment culture at 4 ° C for 21 days. (who.int)
  • The cat was regularly vaccinated for common feline diseases (feline panleukopenia, rhinhotracheitis, calicivirosis, and chlamydiosis) and showed negative test results for retroviral infections. (cdc.gov)
  • However the increase was within the range specified by the Standard Test Method, and proved non-reproducible over two separate experiments. (europa.eu)
  • No known test method can offer complete assurance that Hepatitis B virus, Hepatitis C virus, HIV-1, HIV-2, or other infectious agents are absent. (labquip.net)
  • A: "At the moment, there are cellular based tests on the market. (arminlabs.com)
  • 19 testing and test results. (cdc.gov)
  • Mature DC (mDC) from 18 children with T1DM and 10 age-matched healthy children were tested. (torvergata.it)
  • 1/ A full ADI of 0-0.1 mg/kg body weight was established on the basis of the no-effect levels partially taken from data reported by Industrial Bio-Test Laboratories (IBT). (inchem.org)