The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. (sigmaaldrich.com)
c-Fos was detected immunohistochemically and nicked DNA by in situ TdT-dUTP terminal nick-end labeling (TUNEL). (arvojournals.org)
Double-labeling study revealed that some c-Fos-positive nuclei were also TUNEL-positive nuclei. (arvojournals.org)
In the present study, c-Fos after light damage was detected immunohistochemically, whereas cell death was indicated by TdT-dUTP terminal nick-end labeling (TUNEL). (arvojournals.org)
Double labeling of c-Fos by immunohistochemical method and DNA nicks by in situ TUNEL were also performed. (arvojournals.org)
The cell viability and apoptosis were determined by using adenosine 5′-triphosphate (ATP) assay and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL), respectively. (molvis.org)
Terminal deoxynucleotidyl transferase-mediated biotin nick end-labelling (TUNEL) was performed to assess deoxyribonucleic acid strand breakages as a characteristic of apoptosis. (ersjournals.com)
FD NeuroApop™ Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. (thomassci.com)
Terminal deoxynucleotidyl-mediated dUTP nick end labeling (TUNEL) was used to study cellular apoptosis. (atsjournals.org)
DNA-breaks were visualized by in situ end labeling (terminal deoxynucleotidyl transferase dUTP-biotin nick-end labeling, TUNEL). (uni-luebeck.de)
Using a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay, we found that IDV D/OK and D/600 infections induced apoptosis in epithelial cells lining alveoli and bronchioles, as well as nonepithelial cells in lung tissues. (cdc.gov)
FD Apop™ Kit is designed for the microscopic detection of cells undergoing apoptosis based on the principle of in situ DNA nick-end labeling (TUNEL) technique¹. (fdneurotech.com)
In situ apoptosis detection by TUNEL staining After desired treatment the paraffin-embedded sections of samples were studied by terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling (TUNEL) assay. (healthyconnectionsinc.com)
Heart sections (ten mm) have been utilized to carry out the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay (In-Situ-Cell-Death detection kit, Roche), based on the PPARβ/δ Agonist Gene ID manufacturer's instructions. (atminhibitor.com)
After exposure to isoflurane for 6 h, half the numbers of rats in each group were euthanized, and the hippocampus and cerebral cortex were dissected and examined for apoptosis by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) technique and western blot. (biomedcentral.com)
Importantly, vaccinated animals were protected against intranasal challenge with IDV [3 x 10(5) 50% tissue culture infective dose(s) (TCID50)] D/OK (n = 6) or D/600 (n = 6), based on the absence of viral RNA in necropsied tissues (5 and 7 days postchallenge) using quantitative reverse transcription-PCR and in situ hybridization. (cdc.gov)
Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments. (roche.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Transferase-mediated2
Apoptosis and associated cell types were examined in serial paraffin sections by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and immunohistochemistry. (tmu.edu.tw)
Endoscopic duodenal biopsy specimens from 12 patients with untreated and 12 with treated CD and 12 control subjects were evaluated for enterocyte apoptosis by the terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine triphosphate nick-end labeling assay and for Fas and FasL expression by immunohistochemistry. (nih.gov)
Apoptosis4
The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3'-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis. (thomassci.com)
Ventricular myocardial specimens were investigated by hematoxylin and eosin (HE) and periodic acid Schiff (PAS) staining and immunohistochemical labeling of apoptosis-associated proteins (Bax, Bcl-2, Caspase-3). (uni-luebeck.de)
Subendocardial Purkinje fibers of groups I and II hearts exhibited focal damage, with reduced labeling of apoptosis-associated proteins, glycogen loss, karyopycnosis and increased eosinophilia (15/21 hearts). (uni-luebeck.de)
The FITC-labeled cells undergoing apoptosis were recognized by nuclei with robust green fluorescence. (amparinhibitor.com)
Random-primed2
It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. (sigmaaldrich.com)
Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. (roche.com)
Fluorescein1
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. (sigmaaldrich.com)
Fluorescence2
Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. (sigmaaldrich.com)
Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. (roche.com)
Triphosphate1
However, no differences were observed either in the number of terminal deoxynucleotidyltransferase-mediated d-uracil triphosphate-biotin nick end-labeling-positive cells or viable neurons in the cornu ammonis 1 sector or in the neurologic deficit score when comparing surviving transgenic and nontransgenic rats. (asahq.org)
Probes1
A variety of methodologies for labeling DNA are used to generate end-labeled or continuously labeled probes. (enzolifesciences.com)
Most enzyme-mediated labeling techniques are very much dependent on polymerase activity, which is responsible for incorporation of the labeled nucleotides. (enzolifesciences.com)
The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
Covalently closed1
Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)
Nucleotide3
A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
In the presence of appropriately labeled nucleotide primers, PCR products are labeled as they are being synthesized. (enzolifesciences.com)
Alternatively, the primers themselves may be labeled non-isotopically during their own synthesis, negating the requirement for the inclusion of labeled nucleotide precursors as part of the reaction mix. (enzolifesciences.com)
Reaction1
Worachartcheewan, Apilak Abstract: Pyrrolidines type 2,4-disubstituted (alkyl, aryl or heteroaryl)-6,8-dioxo-3,7-diazabicyclo [3.3.0] octanes (8a-d) were successfully synthesized by an efficient one pot 1,3-dipolar cycloaddition of azomethine ylides (in situ generated from the reaction of aromatic aldehydes and methyl ester of alpha-amino acids) with dipolarophile (N-phenylmaleimide). (tu-dortmund.de)
DTTP1
In addition to the choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP. (enzolifesciences.com)
Fragments1
N-terminal fragments of mutant huntingtin accumulate and form inclusions in the cell nucleus in the brains of patients with HD, as well as in various animal and cell models of HD. (medscape.com)
MRNA1
Nuclear export of mRNA composes one part of a larger network of molecular events that begin with transcription of the mRNA in the nucleus and end with its translation and degradation in the cytoplasm. (biomedcentral.com)
Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
Method1
Nick Translation DNA Labeling System 2.0 to provide a simple and efficient method for generating labeled DNA. (enzolifesciences.com)
Degradation1
Panobinostat triggered terminal myeloid differentiation via proteasomal degradation of A/E9a. (ashpublications.org)
Cells1
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
Translation1
Nick translation (NT) is one of the oldest probe labeling techniques. (enzolifesciences.com)
Variety1
Expertise is available for a variety of labeling procedures. (wisc.edu)
Responsible1
N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. (thermofisher.com)