Template expression rna. Medical search. Frequent questions

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. (nih.gov)
and positive regulation of transcription by RNA polymerase II. (nih.gov)
New England Biosciences makes a similar kit, the WarmStart LAMP Kit for DNA and RNA, that uses a blend of Bst 2.0 DNA polymerase and RTx reverse transcriptase . (genomeweb.com)
Thus, RNA polymerase copies information from template DNA to generate the mRNA template for protein production. (bham.ac.uk)
For instance, H19 is the first lncRNA transcribed by RNA polymerase II and is expressed mainly in human embryonic cells. (jcancer.org)
We propose an underlying mechanism for the favorable effect of longer DNA on gene expression in terms of the enhancement of access of RNA polymerase to the shrunken conformation. (nature.com)
Our goal is to understand the molecular mechanisms whereby the genomic RNA of influenza-like viruses is, on the one hand, the template for transcription and replication of the viral genome by its RNA-dependent RNA polymerase and, on the other hand, an Achilles' heel, whose recognition as non-self can trigger an innate immune response to counter the viral infection. (embl.org)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
This cDNA is then converted into a double-stranded DNA template for transcription using DNA Polymerase and RNase H to simultaneously degrade the RNA and synthesize second strand cDNA. (nih.gov)

The RASL-seq method has been recently described as applicable to massively parallel quantitative analysis of gene expression. (genomeweb.com)
The central dogma of gene expression is that DNA is transcribed into messenger RNAs, which in turn serve as the template for protein synthesis. (nih.gov)
Moreover, zebrafish is very well-suited for systematic analysis of gene expression patterns at transcript level by whole-mount in situ hybridization (WISH), and at protein level by immunohistochemistry/immunofluorescence (IHC/IF). (frontiersin.org)
Transcription is the first step in gene expression. (bham.ac.uk)
each step provides a platform for regulation of gene expression. (bham.ac.uk)
BACKGROUND: We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. (ca.gov)
Coupled with a whole-genome gene expression microarray platform, we routinely obtain expression correlation values of R(2)~0.76-0.80 between individual cells and R(2)~0.69 between 50 pg total RNA replicates. (ca.gov)
CONCLUSIONS/SIGNIFICANCE: In summary, our method facilitates whole-genome gene expression profiling in contexts where starting material is extremely limiting, particularly in areas such as the study of progenitor cells in early development and tumor stem cell biology. (ca.gov)
Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
Many studies have investigated the determining factors that affect gene expression. (nature.com)
AFM observation of the DNA conformation indicates that longer DNA takes shrunken conformation with a higher segment density in the reaction mixture for gene expression, in contrast to the stiff conformation of shorter DNA. (nature.com)
It is expected that the enhancement of gene expression efficiency with a shrunken DNA conformation would also be a rather general mechanism in living cellular environments. (nature.com)
Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
Gene regulation can occur at any point in gene expression, from the start of the transcription phase to the translation phase. (nature.com)
To elucidate the complex mechanism of gene regulation in living cellular systems, it is necessary to closely investigate each phase of gene expression and also to shed light on the cooperative effects between transcription and translation. (nature.com)
Although these factors have been studied extensively, little is known about how the length of the DNA template influences gene expression. (nature.com)
this contributes to shutdown of host-cell gene expression. (embl.org)
And then, we found circIFT80-positive correlation with CTNNB1 ( β -catenin) by sponging miR-142, miR-568, and miR-634 upregulated the gene expression. (hindawi.com)
MicroRNAs are tiny ribonucleic acid (RNA) molecules (~22 nucleotides long) that recently have been found to play important roles in regulating gene expression in eukaryotic organisms, including plants and animals. (sciencedaily.com)
The complex then cleaves the mRNA into smaller pieces, thereby preventing translation of the protein it encodes, and thus inhibiting or "silencing" gene expression. (sciencedaily.com)
There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)
Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)
[ 5 ] Because a microarray can be used to examine the expression of hundreds or thousands of genes at once, it promises to revolutionize the way gene expression is examined. (medscape.com)
[ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)
In an efficient one-step library preparation, this technology eliminates the need for lengthy normalisation and allows for straightforward determination of gene expression values, even at a single cell level. (bioscience.co.uk)
A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease. (genomicglossaries.com)
Using an optimized protocol, the average correlation coefficient of gene expression of 11,123 cDNA clones between amplified and unamplified samples is 0.82 (0.85 when a virtual array was created using repeatedly amplified samples to minimize experimental variation). (biomedcentral.com)
Amplification appears to dampen the variation of gene expression for some genes when compared to unamplified poly(A) + RNA. (biomedcentral.com)
T7 based linear amplification reproducibly generates amplified RNA that closely approximates original sample for gene expression profiling using cDNA microarrays. (biomedcentral.com)
Other examples of specimen requiring amplification for genome-wide characterization of gene expression include purified populations of cells obtained by either flow cytometry, laser capture microdissection, breast ductal or bronchial lavage, or microendoscopy. (biomedcentral.com)

In agreement with GSS activity localization in vivo , we identified six genes encoding arylsulfatase-like enzymes with a predicted C-terminal transmembrane domain, of which five showed GSS activity upon heterologous expression in insect cells. (nature.com)
Here, we describe in detail the spatiotemporal expression of three rprm genes ( rprma, rprmb , and rprm l) within distinct anatomical structures in the developing peripheral and central nervous system. (frontiersin.org)
In most cases, circRNAs usually act as a competitive endogenous RNA (ceRNA) that can absorb miRNAs to regulate the expression of targeted genes [ 9 ]. (hindawi.com)
Auxin influences development by affecting the expression of numerous genes that control the processes of cell division and cell expansion in specific plant tissues at specific stages during the plant life cycle - e.g. for leaves, roots, and floral organs to develop in the correct patterns and correct time sequence. (sciencedaily.com)
Research reported in The Plant Cell shows that microRNAs control the accumulation of transcription factor proteins that regulate the expression of genes in the auxin response pathway. (sciencedaily.com)
Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
One of these targets is the transcription factor AUXIN RESPONSE FACTOR17 (ARF17), which is thought to repress the expression of a number of other genes involved in auxin responses. (sciencedaily.com)
The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
The expression patterns of 16 randomly selected genes were in consistent with that from the Solexa analysis using quantitative reverse-transcription PCR. (biomedcentral.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Less than 4% of genes show changes in expression level by 2-fold or greater after amplification compared to unamplified samples. (biomedcentral.com)
In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)
The program collects whole-genome sequence and other -omics data (In biology, -omics refers to measurable differences or changes in biological molecules, such as genes, metabolites, proteins, and RNA. (cdc.gov)

The ligase was then used to detect Ebola RNA transcripts as well as to detect the presence of point mutations. (genomeweb.com)
The discovery of extensive transcription of large RNA transcripts that do not code for proteins, termed long noncoding RNAs (lncRNAs), provides an important new perspective on the centrality of RNA in gene regulation. (nih.gov)
Some of them are for non-coding RNA transcripts longer than 200 nucleotides, called long non-coding RNAs (lncRNAs) [ 1 , 2 ]. (jcancer.org)
No unspliced RHO transcripts could be detected in RNA from human retina. (molvis.org)

High heat can be used to straighten out RNA molecules, making things like hybridization easier. (genomeweb.com)
The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. (microbiologyresearch.org)
To exclude the influence of supercoiling, we used linear DNA molecules (PCR amplicons or linearized by restriction enzyme) as a template. (nature.com)
Circular RNAs (circRNAs) are a form of noncoding and closed loop RNA molecules and play vital roles in the progression of various types of cancer in humans. (hindawi.com)
MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (sciencedaily.com)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)

Lexogen's Thor technology directly amplifies only the original RNA molecule and produces single-fragments per amplified antisense RNA copy. (bioscience.co.uk)
T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. (biomedcentral.com)

The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
Double-labelled immunofluorescence showed that both p33 and p92 replicase proteins localized to peroxisomes, independently of one another and of the presence of the replication template. (microbiologyresearch.org)
P1234 undergoes a series of precise cleavage events producing four nonstructural proteins (nsP1-4) representing subunits of the RNA replicase . (bvsalud.org)
Genetic testing encompasses a broad range of laboratory tests performed to analyze DNA, RNA, chromosomes, proteins, and certain metabolites using biochemical, cytogenetic, or molecular methods or a combination of these methods. (cdc.gov)

According to one recent review , T4 RNA ligases have become essential for a subset of rapid amplification of cDNA ends (RACE) protocols, 3' RNA labeling, and for the preparation of microRNA sequencing libraries. (genomeweb.com)
Lucigen also provides its OmniAmp enzyme in a kit for loop-mediated amplification of RNA and DNA at temperatures up to about 160 degrees Fahrenheit. (genomeweb.com)
He is also part of a collaboration to develop a ligation-on-RNA method that eliminates the need to create a cDNA library from RNA and merges ligation and amplification steps to detect single point mutations, as previously reported , with applications for low-cost HIV point mutation detection. (genomeweb.com)
METHODOLOGY/PRINCIPAL FINDINGS: The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for global PCR amplification. (ca.gov)
The Luthor 3' mRNA-Seq Library Prep kits from Lexogen combines novel Thor direct RNA amplification technology with an efficient, one-step library preparation method, yielding unprecedented sensitivity and reproducibility for sequencing of ultra-low input RNA and single cells. (bioscience.co.uk)
More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)
We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinomas and high-density cDNA microarrays. (biomedcentral.com)
The fidelity and reproducibility of amplification is not affected by decreasing the amount of input total RNA in the 0.3-3 micrograms range. (biomedcentral.com)
Adding template-switching primer, DNA ligase, or column purification of double-stranded cDNA does not improve the fidelity of amplification. (biomedcentral.com)
The correlation coefficient between amplified and unamplified samples is higher when total RNA is used as template for both experimental and reference RNA amplification. (biomedcentral.com)
RNA amplification techniques have been developed to address the need for sufficient RNA from tiny specimen for microarray hybridization. (biomedcentral.com)

When determining the amount of RNA template, the expression level of the target RNA molecule should be considered, as it affects the subsequent PCR step. (neb.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
They further validate the idea that this knock-in mouse, which preserves expression and binding of RAGE, may be a valuable model for studying this important molecule in disease pathogenesis. (elifesciences.org)
A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule. (msdmanuals.com)

The synthesis of DNA from an RNA template, via reverse transcription, results in complementary DNA (cDNA). (handlebar-online.com)
In parallel, we are doing the same for viral replication, which is unprimed and occurs in two-steps via an intermediate complementary RNA (cRNA). (embl.org)
Messenger RNA (mRNA) from the sample of interest can serve as a template for producing complementary DNA (cDNA) in the presence of a reverse transcriptase enzyme. (medscape.com)

Human immunodeficiency virus (HIV) is a diploid virus: each virion carries two complete RNA genomic strands. (cdc.gov)
The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing 1 - 10 . (cdc.gov)

For example, complex regulation of expression of the nucleoid-associated protein cbpA was found to be dependent not only on sigma factor specificity, but also on binding of the transcription factor Fis upstream of the cbpA start codon. (bham.ac.uk)
These studies are being extended to viral RNPs (the physiological RNA synthesis units) to understand the behaviour of the viral nucleoprotein during replication and transcription and to include host factors important for viral replication. (embl.org)
Briefly, total RNA undergoes T7 oligo(dT)-primed reverse transcription to synthesize first-strand cDNA containing a T7 promoter sequence. (nih.gov)

The enzyme consists of a protein component with reverse transcriptase activity, and an RNA component, encoded by this gene, that serves as a template for the telomere repeat. (nih.gov)
Importantly, a precise temporal description of RPRM protein expression during the development of neuronal structures is still missing. (frontiersin.org)
Herein we examine the temporal and spatial expression patterns of RPRM gene-products (mRNA and protein) during neural development. (frontiersin.org)
In the present study, we evaluated the efficiency of protein translation, focusing on the effect of DNA templates of different lengths on cell-free protein synthesis using a luciferase assay. (nature.com)
The expression level from luc 25.7k is 1000-times higher than that with luc 1.7k, indicating that the protein yield per target gene is increased by 1000-times of magnitude for a longer template, luc 25.7k (Fig. 1 d). (nature.com)
The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
A second report focuses on the function of the protein ARGONAUTE1 (AGO1), a major component of the RNA-induced silencing complex in Arabidopsis. (sciencedaily.com)
Both PWN loading and hypoxia enhanced tracheal elasticity and thickened the apical extracellular matrix (aECM) of the tracheal tubes while a notable upregulated expression of a resilin-like mucin protein Muc91C was observed at the aECM layer of PWN-loaded and hypoxic tracheal tubes. (elifesciences.org)
This unexpected finding suggests that by preserving the expression of RAGE protein (in a dominant-negative form), Ager AHA/AHA mouse might represent a cleaner genetic model to study physiological roles of RAGE in vivo compared to Ager −/− mice. (elifesciences.org)
Det kan röra sig om att kartlägga genom, jämföra expression av budbärar-RNA och proteiner, hitta skadliga mutationer, modellera hur ett protein ser ut, beskriva sammansättningen av ett bakterie-samhälle eller att själv utveckla program med bioinformatisk tillämpning. (lu.se)

Long non-coding RNAs (lncRNAs) are non-coding RNAs longer than 200 nucleotides that function as regulatory factors in many human diseases, including cancer. (jcancer.org)
In breast cancer, lncRNAs are abnormally expressed and demonstrate differential expression in subtypes of tumors [ 9 , 10 ]. (jcancer.org)
Noncoding RNAs, including circRNAs, microRNAs, and lncRNAs, have become a significant focus of research attention due to their crucial role in regulating the progression of multiple malignancies [ 5 ], including bladder cancer, gastric cancer, nasopharyngeal carcinoma, and pancreatic cancer. (hindawi.com)

Labelling with BrUTP showed that peroxisomes were the sites of nascent viral synthesis, whereas in situ hybridization indicated that DI RNA progeny were diffused throughout the cytoplasm. (microbiologyresearch.org)
A very recent report used amino C6dT-modified random hexamers to prime cDNA synthesis in conjunction with aminoallyl-dUTP and increased fluorescence intensity enough such that as little as 1 μg of total RNA from cell lines gave sufficient signal for cDNA microarray hybridization [ 14 ]. (biomedcentral.com)
Fragmentation of the biotin-labeled aRNA prepares the sample for hybridization onto GeneChip 3' expression arrays. (nih.gov)

Sci Rep. 2018) and nucleoside analogue, RNA synthesis inhibitors (Kouba et al . (embl.org)

NEW YORK (GenomeWeb) - Brown University researchers have discovered an RNA ligase that has the ability to function at high temperatures, potentially enabling detection of RNA mutations and improving RNA sequencing. (genomeweb.com)
It could be employed in place of RT-PCR or other reverse transcriptase-based methods to detect RNA via RNA-based probe ligation, and could also be used to detect RNA mutations. (genomeweb.com)
Mutations in the TERC template sequence can be incorporated into the telomeres of human tumors. (nih.gov)

The ligase might also be useful for RNA-mediated oligonucleotide annealing, selection, and ligation, or RASL-seq, Tripathi said, particularly because it is thermostable and template dependent. (genomeweb.com)

One approach to low specimen RNA input has been to use indirect labelling techniques to increase fluorescence signal intensity, such as with aminoallyl nucleotides. (biomedcentral.com)

Telomerase RNA TERC and the PI3K-AKT pathway form a positive feedback loop to regulate cell proliferation independent of telomerase activity. (nih.gov)
However, only the expression of p33, but not p92, increased the number of peroxisomes and induced membrane proliferation. (microbiologyresearch.org)
Ectopic expression of UASR1 promoted proliferation and clonogenic growth of breast cancer cells MCF7 and MDA-MB-231. (jcancer.org)

Two yeast strains were used, differing in the biogenesis of peroxisomes, the organelles supplying the membranous vesicular environment in which CymRSV RNA replication takes place in infected plant cells. (microbiologyresearch.org)
DI RNA replication occurred in yeast cells, as demonstrated by the presence of monomers and dimers of positive and negative polarities. (microbiologyresearch.org)
DI RNA replication also took place in yeast cells devoid of peroxisomes. (microbiologyresearch.org)
The EEEV trans-replicase developed here contributes significantly to research on EEEV, providing a safe and versatile tool for studying the virus RNA replication. (bvsalud.org)

Taken together, our data suggest that UASR1 plays an oncogenic role in breast cancer cells through activation of the AKT/mTOR signaling pathway, being a novel RNA oncogene. (jcancer.org)
The cell counters RNA viruses with innate immune pattern-recognition receptors, such as the RNA helicase RIG-I, which recognise particular viral RNA structural motifs (e.g. 5′ triphosphate-dsRNA) as non-self, thus activating a signalling pathway leading to interferon production and establishment of the anti-viral state. (embl.org)
In conclusion, our work reveals the impacts of circIFT80 as ceRNA in the progression of CRC, by which sponging miR-142, miR-568, and miR-634 enhanced the expression levels of β -catenin and activation Wnt/ β -catenin pathway. (hindawi.com)

This high taneously harbors two different proviruses, one mutation rate, common to most RNA viruses, RNA transcript from each provirus can be encap- permits rapid exploration of nucleotide sequence sidated into a single "heterozygous" virion. (cdc.gov)

Typical microarray labelling procedures require 2-4 μg poly(A) + RNA or 25-50 μg total RNA per cDNA microarray. (biomedcentral.com)
Small tumors identified using early detection strategies may thus be too small to excise a specimen with enough RNA for microarray analysis. (biomedcentral.com)

The group specifically sought out thermostable RNA ligases that might be efficient in nick joining, so that they could be used in RNA detection and sequencing, Tripathi said. (genomeweb.com)

Sequence variations that affect CYP2A13 expression may contribute to interindividual differences in susceptibility to tobacco-related tumorigenesis. (aspetjournals.org)
RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
these regions can between the two RNA templates so that the be conceptualized as "peaks" on a "fitness land- newly synthesized retroviral DNA sequence is scape" of sequence space. (cdc.gov)
We find that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches. (cdc.gov)
After years of development, Reggie now (late 2014) covers registration of the full pipeline of sample preparation, gene sequence and expression analysis, and report creation. (lu.se)

aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)
250 ng total RNA was used to prepare biotinylated aRNA (cRNA) according to the manufacturer's protocol (Affymetrix 3' IVT Express Kit). (nih.gov)

The ability of EEEV P123 to form functional RNA replicases with heterologous nsP4s was more efficient using EEEV template RNA than heterologous alphavirus template RNA . (bvsalud.org)

8 Howard Hughes Medical Institute and Laboratory for RNA Molecular Biology, The Rockefeller University, New York, New York, USA. (jci.org)

According to the study, the addition of single-stranded DNA ligase to the reactions increased template-dependent ligation efficiency. (genomeweb.com)

These findings provide a basis for further studies that associate CYP2A13 haplotypes with incidences of smoking-related lung tumors and for studies on the mechanisms of the low-expression phenotype of the 7520G-containing allele. (aspetjournals.org)

In a study published in RNA Biology the researchers note this is only the third RNA ligase from a thermophile to be studied, and the others are single-stranded ligases that are inefficient at strand joining. (genomeweb.com)
To give a sense of potential applications, a ligase called T4 RNA ligase 2 is similar to KOD1Rnl. (genomeweb.com)

When space (the universe of all possible RNA sequences) this virion subsequently infects a new cell, the (6). (cdc.gov)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

Long non-coding RNAs at work on telomeres: Functions and implications in cancer therapy. (nih.gov)
both E2F1 and c-Myc are stimulating factors of H19 expression, and the H19 lncRNA functions as a downstream effector of c-Myc and E2F1 in tumor development and progression [ 11 ]. (jcancer.org)

Highly parallel genome-wide expression analysis of single mammalian cells. (ca.gov)

Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. (nih.gov)

The importance of these modes of regulation is underscored by the newly recognized roles of long RNAs for proper gene control across all kingdoms of life. (nih.gov)

This amount of poly(A) + RNA or total RNA can be obtained from samples of human tissue that weigh greater than 50-100 mg. (biomedcentral.com)

The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. (nih.gov)
Expression profiles generated from single cells or 50 pg total RNA correlate well with that generated with higher input (1 ng total RNA) (R(2)~0.80). (ca.gov)
Splicing activity was assayed using an in vivo assay in transfected mammalian cells with rhodopsin ( RHO ) and transducin ( GNAT1 ) splicing templates. (molvis.org)
EEEV has a positive-sense RNA genome that, in infected cells , serves as an mRNA for the P1234 polyprotein . (bvsalud.org)

Splicing of RHO intron 3 and GNAT1 introns 3-5 mini-gene templates was inefficient with both spliced and unspliced products clearly detected. (molvis.org)

These miRNAs which targeted β -catenin mRNA were confirmed by dual-luciferase reporter system and RNA-pulldown. (hindawi.com)

The mRNA expression levels of TFFs were examined in the excised CRC specimens obtained from 154 consecutive CRC patients who underwent surgical resection between 2005 and 2007 at our institution. (spandidos-publications.com)
In the present study, we evaluated the mRNA expression levels of TFF1, TFF2 and TFF3 in excised CRC specimens and assessed the correlation between TFF expression and the clinicopathological findings in CRC patients. (spandidos-publications.com)
Bioinformatic approaches were employed for checking the expression of C1QBP in HCC patient samples and the association between C1QBP mRNA expression and survival rates of patients with HCC or the promoter methylation of C1QBP . (jcancer.org)
Furthermore, we also investigated the methylation status and copy number variation of C1QBP and analyzed their correlation with its mRNA expression in HCC patients. (jcancer.org)

We have extended our work on viral polymerases to those of other segmented negative-strand RNA viruses such as the large order of Bunyavirales . (embl.org)

Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)

Finally, we have performed RNA-seq analysis of neutrophils and lungs and found that while Ager −/− mice had a broad alteration of transcriptome in both tissues compared to wild-type mice, the changes of transcriptome in Ager AHA/AHA mice were much more restricted. (elifesciences.org)

The researchers used an "a holistic screening approach" to discover KOD1Rnl, Anubhav Tripathi, a bioengineer at Brown University and principal investigator on the RNA Biology study, told GenomeWeb in an email. (genomeweb.com)

The present study determined the relationship between TFF expression and clinicopathological findings, as well as long-term outcome in CRC patients. (spandidos-publications.com)

The temperature and duration of these steps vary by primer choice, target RNA, and reverse transcriptase used. (handlebar-online.com)

There are few reports on studies that investigated the clinical significance of TFFs expression using excised primary CRC specimens ( 19 ). (spandidos-publications.com)

A high RNA concentration in the PCR may also inhibit the reaction. (neb.com)

Anatomy10

Organisms23

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