Enzyme-linkeCFUsImmunofluorescenceVitroRectalELISABiofilmFetalIsolatesBacterialBacteriaKinaseMatrigelSensitivityPathogenCytokinesRoom temperatureReliesDefectSurvivalSpeciesBacteriumMilliliterLatent formCellsCandidaAgarDegreesAssessIntensive care unitsLaboratoryAnalysisGrowthSamplesMethodConcentrationTestFormationActivityPresentConfirmShowPatientsTimeCultureSingleCell wallStudy
Enzyme-linke1
- Other TB diagnostic methods include blood cultures for extrapulmonary TB (ETB) and serologic tests by Enzyme-linked ImmunoSorbent Assay (ELISA), which do not present sufficient sensitivity and specificity to be useful and determination of adenosine deaminase. (scielo.org.mx)
CFUs4
- It can be represented as a percentage or as colony forming units (CFUs) per microgram of DNA. (wikipedia.org)
- Here is an example of how to calculate transformation efficiency using colony forming units (CFUs): Plate a known number of cells on agar plates containing the appropriate antibiotics. (wikipedia.org)
- For example, if you plate 1x 107 cells and count 1000 colonies, the transformation efficiency is: (1000/1x 107) x 100 = 0.1% Alternatively, CFUs can be reported per microgram of DNA used for the transformation. (wikipedia.org)
- Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)
Immunofluorescence4
- The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
- The underlying mechanisms involved in genomic stability, apoptosis and oxidative stress-related signaling were further analyzed by Western blotting, flow cytometry and immunofluorescence assays. (biomedcentral.com)
- Four rhesus macaques confirmed to be Bartonella -negative by morphologic examination, blood test by nested PCR and serum test by indirect immunofluorescence assay were selected (all tests repeated after a week's interval), deloused, and held in a clean room for 7 days before the inoculation. (cdc.gov)
- Slides of culture cells were fixed in a 1:1 solution of acetone and methanol and prepared for indirect immunofluorescence assay (IFA). (cdc.gov)
Vitro4
- In vitro wound healing assay demonstrated that complexes 2(Eu) and 7(Eu) substantially promoted fibroblast migration compared to the NC. (bvsalud.org)
- Additional in vitro and in vivo data show that PilT and PilU act as independent homo-hexamers that may form a complex to facilitate pilus retraction. (biorxiv.org)
- Variables to be examined include agent concentrations, incubation duration and temperature, cell density, sequence of treatment, and number of cycles of in vitro treatment. (arizona.edu)
- Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
Rectal1
- The rectal temperature and survival were evaluated during the study. (bvsalud.org)
ELISA1
- Procedures -PI status was determined by use of an antigen-capture ELISA (ACE) and confirmed by use of a second ACE, reverse transcriptase-PCR assay of sera, immunohistochemical analysis, and virus isolation from sera. (avma.org)
Biofilm4
- Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE -mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. (biomedcentral.com)
- Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
- This mutant had obvious differences in colony morphology (smaller plaque, edge smooth and round) and defects in biofilm formation in contrast with the wild-type (WT) strains. (biomedcentral.com)
- The disk diffusion method was applied to determine antimicrobial resistance patterns according to the Clinical and Laboratory Standards Institute, and the microtiter plate assay was managed to explore the biofilm formation. (japsonline.com)
Fetal1
- The placenta is a dynamic organ consisting of maternal and fetal tissues, forming an impermeable physical and biological barrier that protects the fetus against pathogens [ 1 , 2 ]. (biomedcentral.com)
Isolates2
- This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
- This study included 74 P. aeruginosa isolates recovered from 412 clinical isolates taken from patients urine admitted to wards/intensive care units (ICUs) and who acquired nosocomial infections after excluding signs or symptoms of infection at the time of admission. (japsonline.com)
Bacterial1
- The spleen was used to assess the bacterial load using colony-forming unit assay. (bvsalud.org)
Bacteria1
- Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. (scielo.br)
Kinase4
- 2014) observed that lethally Rabbit polyclonal to ZFP2 gamma irradiated cells were metabolically active and exhibited comparable tumor growth inhibition Entinostat kinase activity assay and induction of cytokines compared to live cells. (cancer-ecosystem.com)
- These bacteriophages require the host cells machinery to varying degrees to Entinostat kinase activity assay produce progeny phage particles. (cancer-ecosystem.com)
- The culture was allowed to grow at Entinostat kinase activity assay 35C to an OD600 of ca. 0.5 resulting in approximately 1 108 colony forming units (CFU)/ml. (cancer-ecosystem.com)
- The positive Entinostat kinase activity assay control samples were packaged the same way as the experimental samples and were transported to the irradiation facility to eliminate possible differences in survival due to transport and handling. (cancer-ecosystem.com)
Matrigel1
- Using matrigel plug assay, we found that antagomiRs specific for miR-130a and miR-495 significantly reduced angiogenesis in vivo . (oncotarget.com)
Sensitivity2
- Cellular sensitivity is an approach to inhibit the growth of certain cells in response to any non-permissible conditions, as the presence of a cytotoxic agent or due to changes in growth parameters such as temperature, salt, or media components. (bio-protocol.org)
- Yet this sensitivity may lead to false positives as most assays are not designed to determine whether the DNA is from a viable cell. (surlybrewing.com)
Pathogen1
- According to the World Health Organization (WHO), TB is responsible for about 1.6 million TB deaths and 10 million (5.8 million men, 3.2 million women, and 1.0 million children) new cases have been detected in 2017 and more than a third of the world population is hosting Mycobacterium tuberculosis , the causative pathogen of TB, in its latent form ( 1 , 2 ). (frontiersin.org)
Cytokines1
- 1 , 2 In addition, mRNA encoding the cytokines interleukin 2 (IL-2) and granulocyte macrophage colony stimulating factor (GM-CSF) are increased at sites of disease activity, suggesting local activation of antigen specific T cells. (bmj.com)
Room temperature2
- The BDOC assay was performed in 40-ml vials, with glass fiber filtered, pasteurized test water, inoculated with the indigenous microflore from a stream, and incubated for 28 days in the dark et room temperature. (stroudcenter.org)
- Commercially pure Ti sheets were anodized in an electrolyte containing ethylene glycol, distilled water and ammonium fluoride at room temperature to produce TiO 2 nanotubes. (hindawi.com)
Relies1
- Fluorescent assay - This method relies on the use of a plasmid that contains a fluorescent protein or reporter gene. (wikipedia.org)
Defect1
- The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
Survival1
- After incubation, cells will be plated for CFU-GM and CFU-Leuk assays to determine survival of committed hematopoietic progenitors and log-kill of AML cells, and optimal conditions for antileukemic effect with stem-cell sparing. (arizona.edu)
Species2
- Rapid identification and differentiation of Bartonella species using a single-step PCR assay. (cdc.gov)
- Anodization leads to an oxidation of metal species that form a solid oxide on the metal surface. (hindawi.com)
Bacterium2
- We further developed an S . ruminantium -specific PCR assay to aid in the identification of this bacterium. (biomedcentral.com)
- 1 BoNTs are produced In Taiwan (China), two outbreaks have been recorded, by Clostridium botulinum , a Gram-positive, rod- one caused by type A botulism in nine patients who con- shaped, anaerobic, spore-forming, motile bacterium. (who.int)
Milliliter1
- Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)
Latent form1
- PPO is often isolated from plant tissues in a latent form, which can be activated by a range of treatments, including proteolysis, aging, and incubation with detergents. (ashs.org)
Cells7
- Incubate the plates for a period of time (usually overnight) at the appropriate temperature and conditions for the cells. (wikipedia.org)
- To calculate the transformation efficiency, divide the number of colonies by the number of cells plated and multiply by 100. (wikipedia.org)
- According to the viability assays, 3-O-acetyl-11-keto-ß-Boswellic acid (AKBA) showed more toxicity against MDA-MB-231 cells when compared with the 3-O-acetyl-ß-Boswellic acid (ABA). (bvsalud.org)
- The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
- Colonies of B. quintana harvested after 5 days of the growth on a chocolate agar plate were inoculated onto Vero E6 cells. (cdc.gov)
- When compared to air sampled prior to ignition, samples of the air-smoke mixtures contained fivefold higher concentrations of microbial cells (6.7 ± 1.3 × 10 4 cells m −3 ) and biological INPs (2.4 ± 0.91 × 10 3 INPs m −3 active at temperatures ≥ −15 °C), and these data significantly positively correlated with PM 10 . (nature.com)
- Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
Candida1
- The mechanism of action of 5-hydroxy-7,4'-dimethoxyflavone as a potent antifungal agent was investigated by determining its inhibitory activity on Candida albicans drug efflux pumps using the ciprofloxacin assay. (biomedcentral.com)
Agar1
- We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)
Degrees3
- The samples were incubated aerobically overnight at 37 degrees-C and colonies were enumerated in terms of colony forming units (CFU). (cdc.gov)
- Samples were collected at 65 percent relative humidity, 27.8 and 22.8 degrees dry, and wet bulb temperatures. (cdc.gov)
- Enzyme activity was assayed under 55 percent relative humidity, 32.3 and 25.0 degrees dry, and wet bulb temperatures. (cdc.gov)
Assess1
- Colony-forming unit (CFU) assays may also be used to assess editing efficiency by plucking and isolating DNA from individual colonies, followed by PCR analysis. (stemcell.com)
Intensive care units1
- In October 1981, Part II of the Guideline for Hospital Environmental Control, which contained the sections 'Housekeeping Services and Waste Disposal,' 'Laundry Services,' 'Intensive Care Units,' and 'Pharmacy,' was published. (cdc.gov)
Laboratory1
- Pasteurized water was sent to the Stroud Water Research Center for AOC, BDOC, and DOC analyses, and to the Risk Reduction Engineering Laboratory for the CGR assay. (stroudcenter.org)
Analysis1
- The kidney, liver, and lungs were harvested for histopathological analysis, and blood was obtained for cytokine and liver enzyme assay. (bvsalud.org)
Growth4
- The CGR assay indicated that 79 % of the test waters did not promote coliform growth, 7 % were strongly growth promoting, all from surface water sources, and 14 % were moderately growth promoting. (stroudcenter.org)
- In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
- This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies. (jove.com)
- The main advantage of this protocol is that it is less labor-intensive and time-consuming than the traditional method of counting colony forming units to estimate the intracellular growth of Mycobacterium tuberculosis. (jove.com)
Samples2
- Samples were incubated at 20°C for 5 days and response determined from colony forming units. (stroudcenter.org)
- Water samples were assayed for potential respiratory viruses (adenoviruses and enteroviruses), but none were detected. (cdc.gov)
Method1
- Time-kill assays were conducted for the combinations using the colony counting method. (biomedcentral.com)
Concentration1
- Depending on the anodization conditions (potential, nature of the electrolyte, concentration of the electrolyte, temperature, potential sweep rate, pH, and anodizing time) [ 12 , 13 ], the solid oxide layer can be either compact, or nanotubular [ 14 ]. (hindawi.com)
Test2
- Test waters were transferred to 40-ml vials, pasteurized, inoculated, and incubated at 15°C. Colony forming units was the test parameter. (stroudcenter.org)
- La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
Formation1
- 8+ avaient une corrélation positive importante avec les cellules CD34+ apoptotiques et la capacité à stimuler la formation de colonies. (who.int)
Activity2
- The isolated compounds were then investigated for their antifungal activity using broth dilution assay. (biomedcentral.com)
- Leaf extract browning assays indicated that tobacco leaves lack the endogenous phenolic substrates required for significant jrPPO1 activity and quinone production in planta. (ashs.org)
Present1
- However, the tubercle bacillus may be present in a form without a cell wall and thus not detectable by conventional microbiological methods. (bmj.com)
Confirm2
- Enzyme assays confirm that PTC-847 and PTC-672 inhibit Ng RNR and reveal that allosteric effector dATP potentiates the inhibitory effect. (elifesciences.org)
- An initial investigation was conducted to confirm the outbreak and to form hypotheses about the potential causes, followed by a case-control assessment of the plausible causative food item. (who.int)
Show2
- With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
- Resistance mutations in Ng map to the N-terminal cone domain of the α subunit, which we show here is involved in forming an inhibited α 4 β 4 state in the presence of the β subunit and allosteric effector dATP. (elifesciences.org)
Patients3
- RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
- Les patients qui ont accepté de participer après apptéléphonique ont été inclus de janvier à mai 2021.Les variables d'étudeétaient: âge, sexe, acuité visuelle (AV), caractéristiques de la LCET selon Diallo, pronostic fonctionnel et anatomique en post puberté (plus de 15 ans). (bvsalud.org)
- Il s'agit d'une étude rétrospective descriptive et analytique, multicentrique portant sur des patients de moins de 5ans pris en charge pour une affection neurochirurgicale de Janvier 2019 à Décembre 2021 à Libreville. (bvsalud.org)
Time1
- 35 kg/m(2) each incremental BMI unit increase was associated with greater incremental total OR time increases. (stanford.edu)
Culture2
- This can be calculated by multiplying the number of colonies by the volume of the culture plated and dividing by the amount of DNA used. (wikipedia.org)
- We observed that the colony morphology of aceE -mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis . (biomedcentral.com)
Single1
- Type IV pili are composed almost exclusively of a single protein called the major pilin, which forms a helical fiber that extends from the cell surface [ 6 ]. (biorxiv.org)
Cell wall1
- These collectively form a thick, dense, poorly permeable cell wall, which not only allows mycobacteria to resist the dry environment and harmful chemicals but also allows it to reproduce in the macrophages [ 4 , 6 ]. (biomedcentral.com)
Study1
- This was a longitudinal descriptive study conducted in the ophthalmology unit of the Yaoundé Gyneco-Obstetric and Pediatric Hospital. (bvsalud.org)