Techniques frozen sections. Medical search. Frequent questions

For electron microscopy the sections are considerably less than one ten-thousandth of a millimeter (0.1 micron, m) thick. (columbia.edu)
As new research techniques, such as electron microscopy, immunohistochemistry , and molecular biology have expanded the means by which biomedical scientists can study disease, the definition and boundaries of investigative pathology have become less distinct. (bionity.com)
In addition to the structome analysis by serial ultrathin sectioning, PCM (phase contrast microscopy) and SEM (scanning electron microscopy) examinations were performed on the same bacterial sample because the fundamental morphological studies were achieved with these observation techniques. (harvard.edu)

Frozen sections of tissue and cytological specimens are also suitable for laser microdissection. (concordia.ca)
For this reason and because of the body of literature from hundreds of different Chlamydia studies that demonstrate a tremendous variability in diagnosing, identifying, and localizing Chlamydia in clinical specimens, we went to the extreme in our investigations by using a large variety of techniques on different clinical specimens. (alzforum.org)
We cannot stop short of intensifying these studies and we must work to develop standard techniques that can be applied by laboratories analyzing clinical specimens. (alzforum.org)
Electron energy loss spectroscopy allows one to look at chemical binding states of 100-nm thin section specimens, with about 0.5-eV energy resolution in most instruments and a spatial resolution of 5 nm or better. (nationalacademies.org)
When possible it is preferable to submit the specimens unfixed immediately to the pathologist who can select the most appropriate technique for handling each individual specimen. (bccancer.bc.ca)
For microtensile test, samples were prepared into blocks of enamel/resin, which were sectioned to obtain hourglass-like specimens. (bvsalud.org)

The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. (wikipedia.org)
Other techniques involve dehydration in alcohols and infiltration with paraffin, or some similar agent - a process called embedding. (columbia.edu)
The LCM technique can be used on formalin-fixed, paraffin-embedded (FFPE) sections. (concordia.ca)
Students will perform histological procedures involving tissue specimen including: fixation, embedding, microtomy, frozen sectioning, and staining paraffin embedded tissue. (asurams.edu)
The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. (southampton.ac.uk)

Frozen section may be considered if the Surgeon is proceeding directly to further definitive surgery after excisional biopsy. (bccancer.bc.ca)

Tissues are freshly snapped frozen in OCT blocks, sectioned at a thickness of 7-10microns and sections are mounted on positively charged slides (2 sections per slide). (delos.info)
For fixed frozen sections, tissues are fixed in 10% neutral buffered formalin or 4% paraformaldehyde, cryoprotected in sucrose solution, snapped frozen in OCT and sectioned as above. (delos.info)
In order to study tissues with a microscope they must be preserved (fixed) and cut into sections thin enough to be translucent. (columbia.edu)
Finally, in order to preserve the section which has been made from a block of fixed tissues and stained, it is mounted on a glass slide and covered with a thin cover glass by means of a transparent substance which hardens and seals the preparation to make it permanent. (columbia.edu)
The option to process both FFPE slides (human and mouse only) or Fresh frozen tissues is available. (gulbenkian.pt)
In our PCR analysis, we used frozen tissue samples to minimize the difficulties encountered in PCR from fixed and embedded tissues. (alzforum.org)

Frozen tissue sections are prepared in RNase-, protease-free environment and are ideal for immunohistochemistry and in situ hybridization. (delos.info)

The facility offers a wide variety of histological studies, ranging from routine techniques and services to the development of new approaches (IHC, IF,…), experimental pathology studies, reports, and consultancy services (mouse phenotyping, animal models of disease, drug efficacy and non-GLP toxicity studies). (irbbarcelona.org)
The unit provides a well-equipped laboratory for performing histological analysis for diagnostic and research purposes, as well as staff with the necessary expertise to offer advice and guidance on histology and cytological techniques. (otago.ac.nz)

52 ] The current modalities available for intraoperative brain tumor examination include stimulated Raman histology, frozen sections (hematoxylin and eosin-stain), and cytological methods. (surgicalneurologyint.com)

Sections 3 to 10 microns (3 to 10 thousandths of a millimeter) in thickness are cut on steel knives mounted in an instrument called a microtome, which has a precise mechanical advance. (columbia.edu)

The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. (wikipedia.org)
The microtome can be compared to a very accurate "deli" slicer, capable of slicing sections as thin as 1 micrometre. (wikipedia.org)
Subsequently, it is cut frozen with the microtome portion of the cryostat, the section is picked up on a glass slide and stained (usually with hematoxylin and eosin, the H&E stain). (wikipedia.org)
Lung lobes obtained at necropsy from 100 patients were frozen at -70 degrees C and sectioned as a whole in a sledge microtome as used on complete frozen animals. (bmj.com)

Do you free-float the sections or mount them on slides? (utsouthwestern.edu)

Using thick resin sections and electron tomography allowed us to capture a much better three-dimensional view of the extensive membrane compartments within the matrix of the PAS and a massive elaboration of membrane tubules within the arbuscule," Berg said. (danforthcenter.org)

Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after formalin fixation, and pathologist Dr William Welch, also at Hopkins, experimented with Cullen's procedure but without clinical consequences. (wikipedia.org)
The process of fixation is briefly described in the next section. (columbia.edu)
Following fixation, blocks of tissue must be cut into thin sections. (columbia.edu)

The distribution of neoplastic and normal B-lymphoid cells in nodular lymphomas: use of an immunoperoxidase technique on frozen sections. (bdbiosciences.com)
6 months, processed by dRIT in Prevention (CDC) by incorporating various components of the field, and placed into glycerol solution for shipment to existing immunoperoxidase techniques ( 6 ). (cdc.gov)

[2] [8] A student of Virchow's, Julius Cohnheim (1839-1884) combined histology techniques with experimental manipulations to study inflammation , making him one of the earliest experimental pathologists. (bionity.com)
With our specialised equipment and expert team of scientists and technicians, we have the flexibility to tailor our histology techniques to suit any research project. (otago.ac.nz)

We used cryofixation to ultra-rapidly freeze the root tissue. (danforthcenter.org)
E. coli cells were cut into 65 nm thick serial ultrathin sections after rapid cryofixation and freeze-substitution. (harvard.edu)

Section One: Theoretical and experimental aspects of food freezing. (chipsbooks.com)

The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. (wikipedia.org)
The intraoperative consultation is the name given to the whole intervention by the pathologist, which includes not only frozen section but also gross evaluation of the specimen, examination of cytology preparations taken on the specimen (e.g. touch imprints), and aliquoting of the specimen for special studies (e.g. molecular pathology techniques, flow cytometry). (wikipedia.org)
Usually blocks are taken perpendicular to the margin but if taken 'en face' this must be recorded in the dictation or on a specimen diagram. (bccancer.bc.ca)

Using high pressure jets (~2000 atmospheres) of liquid nitrogen the roots were frozen in six milliseconds without cellular ice crystal formation, and providing state of the art preservation of cellular structure. (danforthcenter.org)
This letter is in response to the recent article in Journal of Clinical Microbiology (38[2]:881-882, 2000) entitled 'Failure to detect Chlamydia pneumoniae in brain sections of Alzheimer's Disease Patients' by Gieffers et al. (alzforum.org)

This allows for transcriptome-wide mapping of genes expression onto tissue sections, with concomitant H&E or IF staining. (gulbenkian.pt)

Also I did some slicing today with some of my frozen brains and kept the block in the cryostat till it came to temperature and as compared to before I think I can say that the slices came out pretty well. (utsouthwestern.edu)
Are you using an anti-roll plate when sectioning on the cryostat? (utsouthwestern.edu)

The principal use of the frozen section procedure is the examination of tissue while surgery is taking place. (wikipedia.org)
Histology services prepare samples for analysis using a variety of techniques for subsequent examination. (otago.ac.nz)
The process of obtaining a frozen section, the gold standard for intraoperative histopathological examination, is time-consuming and can delay surgical treatment. (surgicalneurologyint.com)

It addresses the principles of freezing and the concepts of quality from a variety of different perspectives, including: technological (mechanical and cryogenic methods of freezing) categorical (classification of quality losses) analytical (measurement of quality), theoretical (model building), applied (preventive treatments), and administrative (policy). (chipsbooks.com)
The mechanical characteristics, microstructure, water absorption, freeze-thaw and fire resistance of the modified AAMs were evaluated. (imperial.ac.uk)
Results showed that brick-based one-part AAMs can achieve mechanical properties, pore structure, water absorption and freeze-thaw resistance comparable to fly ash-based AAM while having 65% better fire resistivity. (imperial.ac.uk)

Elaborates on treatments, outside the freezing process, which may minimize quality losses during freezing and storage. (chipsbooks.com)
Section three: Techniques to minimize quality losses. (chipsbooks.com)

Provides a thorough discussion of the technological and fundamental features of freezing, providing a solid background for the remaining sections which emphasize the effects on food quality. (chipsbooks.com)

Students will demonstrate proper laboratory safety techniques including the handling of chemicals and biohazardous waste. (asurams.edu)
The objectives were to validate the dRIT evaluated under field and laboratory conditions to detect as a field test for rabies surveillance and evaluate the dRIT rabies virus antigen in frozen and glycerol-preserved field on glycerol-preserved field samples. (cdc.gov)
The analytical methods are described in the Description of Laboratory Methodology section above. (cdc.gov)
Laboratory team performance is monitored using several techniques. (cdc.gov)

this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. (scielo.br)
Emergent frozen section can help confirm diagnosis in early cases. (orthobullets.com)
In addition, estrogen receptor staining may be a useful technique in evaluating metastases in patients with unknown primaries in whom the possibility of metastatic breast carcinoma is in the differential diagnosis. (bccancer.bc.ca)
For the routine diagnosis of rabies, glyc- erol saline is a convenient preservative in situations in which refrigeration or freezing facilities are not promptly available ( 7 ). (cdc.gov)
A brain smear is a simple, rapid, and cost-effective technique, particularly important in the diagnosis of brain tumors. (surgicalneurologyint.com)

The Freeze Panes option is intended for use in more complex situations in which you need to freeze multiple rows or columns. (redmondmag.com)

The method of processing is usually done with the bread loafing technique. (wikipedia.org)
Companies in this industry make fresh and frozen bread as well as cakes, pies, and doughnuts. (thermofisher.com)

The patterns of the printcultures were reproducible in successive sections, and the quantity of the bacterial species could be assessed. (bmj.com)

Overview of physical/chemical aspects of freezing. (chipsbooks.com)
Chemical measurements of frozen foods. (chipsbooks.com)

Not previously found in other publications, this book offers an enhanced concentration on the principles of frozen food quality. (chipsbooks.com)

Our findings hold such great implications as to how inflammation in the AD brain may be triggered by infection with C. pneumoniae that we must demand that studies to replicate and/or validate our first report should be performed with the rigor and comparable techniques that will provide data that can truly be compared and analyzed. (alzforum.org)

Incorporating bricks as aggregate resulted in a maximum improvement of 17% and 27% in the AAMs' compressive and flexural strength levels, respectively, and a general enhancement in the freeze-thaw resistance with showing no reduction in compressive strength after exposure to elevated temperature. (imperial.ac.uk)

With the development of new surgical techniques in breast cancer, such as oncoplastic breast surgery, increased knowledge of risk factors for poor satisfaction with conventional breast-conserving surgery (BCS) is needed in order to determine which patients to offer these techniques to. (springer.com)

Cakes and Pies, Fresh and Frozen - What's The Best Inspection Technique? (thermofisher.com)
If you understand the challenges involved, consider 'product effect', identify fresh vs frozen differences, and ensure packaging and trays are considered, then delivering the perfect pie to the customer will be a piece of cake. (thermofisher.com)
One way is to make a firm block by freezing fresh or fixed tissue. (columbia.edu)
It can be performed on fresh tissue, frozen tissue, core biopsies scrapings obtained from the surface of small lesions or frozen sections, and on aspirates with reliable results. (bccancer.bc.ca)

Role of packaging in quality preservation of frozen foods. (chipsbooks.com)
The team's research built upon previous studies by deploying high pressure freezing techniques that provided more accurate preservation of cell structure. (danforthcenter.org)

Ultrasonography of the neck is a safe and widely used technique for localization of abnormal parathyroid glands. (medscape.com)
a version of this technique is widely employed by modern pathologists to render diagnoses and provide other clinical information intraoperatively. (bionity.com)

I'll share the experiences and techniques I've gained in my long career as a chef. (cindysbackstreetkitchen.com)

Excel refers to the process of locking a row or a column as freezing. (redmondmag.com)

this compound is known by many names and when frozen has the same density as frozen tissue. (wikipedia.org)

If the percentage of breast volume excised is estimated to exceed 20%, other techniques, such as oncoplastic breast surgery, with or without contralateral surgery, or mastectomy with reconstruction, may be considered. (springer.com)
2 cm in diameter, as adjuvant therapy following neoadjuvant chemotherapy and surgery (see sections 4.4 and 5.1). (who.int)

As such, I used a few different techniques to make the spreadsheet easier to use. (redmondmag.com)
Incidentally, if you make a mistake, you can undo the operation by going back to the Freeze Panes icon and selecting the unfreeze option. (redmondmag.com)

Analysis of developed sections showed that at 5 min, there was a rapid accumulation of 14C-activity in the kidney cortex and stomach walls. (cdc.gov)

We can carry out the 'wet lab' aspects of your project and perform processing, tissue embedding, sectioning, and routine and specialised staining. (otago.ac.nz)

It can be a viable and accessible alternative to more traditional methods such as frozen sections and can be incorporated into neurosurgical practice in LMICs as a reliable and efficient diagnostic tool. (surgicalneurologyint.com)

Immunocytochemical staining is the standard technique to test for estrogen receptors. (bccancer.bc.ca)

The animals were embedded in carboxymethyl cellulose and frozen immediately. (cdc.gov)
Frozen animals were sectioned and processed using whole-body autoradiographic techniques. (cdc.gov)

Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. (scielo.br)

Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice and are good for one year if properly stored. (delos.info)

Western blotting is an important technique used in cell and molecular biology. (delos.info)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

An upgrade of the technique Visium HD, bringing single-cell resolution, will be released in the coming months. (gulbenkian.pt)
P.285 right column 2nd paragraph: 'Ribosomes as electron dense particles with 20 nm diameter in the cytoplasm of the cell in each serial ultrathin section were enumerated [refs 16,22] using menus and macro of ImageJ/ Fiji [ref 21]. (harvard.edu)

Many cake and pie products are frozen immediately after production, so some bakers choose to inspect after the items are case packed using an X-ray unit that can accommodate the case size. (thermofisher.com)

Frozen stress techniques in photoelasticity have been used very much to obtain the stress fields developed in the models. (witpress.com)

The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. (wikipedia.org)

As an alternative, you could also select the column just to the right of the one that you want to freeze. (redmondmag.com)

Anatomy5

Diseases8

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes2

Disciplines and Occupations4

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