ELISAImmunoassayMethodsSuitableAntibodiesImmunocytochemistryHistologySpecificitySensitiveApproachesAssayResultsQuantitativeReceptorSignificantlyDiagnostic techniquesMolecularResearching the developmentSpecificDevelopmentExtremelyAdvancesWesternMethodProductionBloodControlDiagnosisFlowConcentrationLevelResearchVarietyPlasmaMultiplePopulation
ELISA5
- [ 9 ] and cross-linked N-terminal telopeptide (NTX) of type 1 collagen by enzyme-linked immunosorbent assay (ELISA) technique (NTX, Osteomark). (medscape.com)
- These techniques were based on di- ple and quantitative postadsorption ELISA for distinguishing agnostic approaches such as radioimmunoassays and neu- between monkeypox and vaccinia infections. (cdc.gov)
- Serologic techniques included identification of monkeypox peptide-specific immunoglobulin (Ig) G by peptide ELISA and kinetic analysis of IgG to orthopoxvirus in paired plasma samples (2-4 mo and 1 y postinfection) by endpoint ELISA. (cdc.gov)
- For this reason, the core encourages investigators to perform a secondary screening technique, such as Western blot, dot blot, histology, immunoprecipitation or radioimmunoassay, on candidate ELISA-positive supernates. (mayo.edu)
- As a result, non-radioactive labeling techniques such as enzyme-linked immunosorbent test (ELISA) have been developed. (microbiologynote.com)
Immunoassay2
- The FIA assay resembles the radio-immunoassay (RIA) in technique and sensitivity without the potential health hazards associated with the handling of isotopes in the laboratory. (cdc.gov)
- In conclusion, radioimmunoassay (RIA) is a strong immunoassay technique that uses radioisotopes as labels to detect and quantify antigens or antibodies. (microbiologynote.com)
Methods3
- The techniques Materials and Methods described provide independent diagnostic tests suitable for retrospective analysis of monkeypox outbreaks. (cdc.gov)
- METHODS: Radioimmunoassay and RT-PCR technique were used to detect two cytokines' level and existence of receptor for AGE (RAGE). (chinaphar.com)
- Assays have been developed on mass spectrometry platforms including surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem-MS techniques (LC-MS/MS). Some methods use an internal standard, either hepcidin analogs or bioactive hepcidin-25 synthesized with stable isotopes. (haematologica.org)
Suitable1
- 3. Its rapidity and relative inexpensiveness makes radioimmunoassay at present the most suitable technique for large-scale population screening. (portlandpress.com)
Antibodies5
- Despite the tralization assays that used a preadsorption step to remove diffi culty of diagnosing clinically inapparent monkeypox in or reduce cross-reactive orthopoxvirus antibodies before previously vaccinated persons, this technique exhibited detection of species-specifi c antiviral antibodies ( 13 - 15 ). (cdc.gov)
- The Western blot is a technique used to identify candidate hybrids, clones, subclones and monoclonal antibodies of interest that recognize and bind to one or more specific molecular weight proteins of interest. (mayo.edu)
- In fact, the technique of component resolved diagnosis, measuring the specific IgE antibodies to individual allergen molecules (natural or recombinant), rather than to the entire sources containing them, provides more precise data for the diagnosis. (dovepress.com)
- The radioimmunoassay (RIA) technique is used to evaluate the presence and concentration of antigens or antibodies in a material. (microbiologynote.com)
- Radioimmunoassay (RIA) is a highly sensitive laboratory technique that uses radioisotopes to detect and measure the concentration of antigens or antibodies in a sample. (microbiologynote.com)
Immunocytochemistry1
- We make use of biochemical and cell biological techniques, such as radioimmunoassay, high pressure liquid chromatography, fluorescence immunocytochemistry and receptor binding assays, in order to examine the trafficking of the mutant p roinsulins. (nih.gov)
Histology1
- Every day there is something new to discover, for instance, the different color patterns of my charismatic lab frogs or using histology techniques to analyze the intricacy of their larynx anatomy. (berkeley.edu)
Specificity2
- Antibody to 5-FU in the sera of sensitized animals has been suggested by an immunoenzymatic staining technique and its specificity confirmed in a radioimmunoassay. (jci.org)
- The principle of radioimmunoassay (RIA) is based on competitive binding, specificity, and sensitivity. (microbiologynote.com)
Sensitive3
- Sensitive radioimmunoassay techniques were developed for diagnostic and medical use. (wikipedia.org)
- Radioimmunoassay (RIA) - RIA technique is an extremely sensitive and specific requiring specialized equipment. (myhealth.gov.my)
- These systems provide sensitive results, but the special handling and disposal problems of 125 I reagents have discouraged continued use of this technique. (bio-rad.com)
Approaches1
- A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Assay2
- During the years 1989-1994, during the work for my graduation and PhD theses, my research activities were aimed at analysing the role of opioid peptides in cell cultures and in and native tissues, with molecular biology techniques, such as RNAse protection assay, with antisense technologies, and with radioimmunoassays. (unibo.it)
- The assay is based on the use of 125 -iodine-labelled digoxin and of a gel equilibration technique for the separation of antibody-bound and free digoxin. (bmj.com)
Results3
- This publication was produced under an IAEA coordinated research project and details the results of the work completed by the project counterparts in developing radiometric and complementary techniques for veterinary drug residues. (iaea.org)
- Her pioneering work results in the development of several new techniques for administering cancer chemotherapy. (aafp.org)
- The results obtained compare closely with those by a conventional radioimmunoassay and the technique is sufficiently simple to enable its performance by relatively inexperienced laboratory staff. (bmj.com)
Quantitative1
- This combination of procedures thus provides a new quantitative technique for studying the properties of the gastrointestinal hormone-containing granules, and should therefore be of value in investigating pathophysiological problems in a variety of gastrointestinal diseases. (bmj.com)
Receptor1
- In addition, during this time frame, I also evaluated pharmacologic affinities of new compounds using receptor binding techniques. (unibo.it)
Significantly1
- The molecular allergy technique, currently defined as component-resolved diagnosis, significantly improved the diagnosis of allergy, allowing for differentiation between molecules actually responsible for clinical symptoms (genuine sensitizers) and those simply cross-reacting or shared by several sources (panallergens), thus influencing the appropriate management of a patient's allergy. (dovepress.com)
Diagnostic techniques2
- Advances in screening and diagnostic techniques allow earlier detection of cancer and timely initiation of treatment. (laskerfoundation.org)
- Historically, time-consuming and labor- enpox, developing multiple diagnostic techniques that can intensive strategies such as cross-adsorbed neutralization be used to identify and confi rm monkeypox is crucial. (cdc.gov)
Molecular3
- Creager is the author of The Life of a Virus: Tobacco Mosaic Virus as an Experimental Model, 1930-1965 (University of Chicago Press, 2002), on snuff mosaic virus As the field of molecular biology developed, Tobacco mosaic virus (TMV) became a paradigmatic experimental model for the study of viruses and the development of new scientific techniques. (wikipedia.org)
- Major techniques used for this work include monitoring of hormone levels in the blood by radioimmunoassay, quantification of neuronal activity in specific brain regions by neurochemical and molecular biology techniques, identification of specific populations of neurons in the hypothalamus by immunohistochemistry and in situ hybridisation, and use of transgenic mice to study hormone effects on brain function. (otago.ac.nz)
- Work on MAPKinases in nicotine dependence models with neuroanatomical and molecular biology techniques. (unibo.it)
Researching the development1
- As of 2015[update] Creager is researching the development of techniques for detecting environmental carcinogens and their regulation between 1960 and 1990. (wikipedia.org)
Specific1
- Analytical subcellular fractionation techniques in combination with specific radioimmunoassays have been used to investigate the properties of the hormone-containing granules in human jejunal biopsy specimens. (bmj.com)
Development2
- Beadle and Ephrussi used their technique to study the development of the eye and eye pigment. (asu.edu)
- In the 1950s, Dr. Yalow pioneered the development radioimmunoassay, a technique for detecting extremely small amounts of hormones in the blood that would eventually earn her the Nobel Prize. (riverdalepress.com)
Extremely1
- An extremely rapid radioimmunoassay for digoxin is described which is precise over the range of concentrations required to determine whether, or not, a patient has digoxin toxicity. (bmj.com)
Advances1
- She developed the technique of radioimmunoassay, one of the most important clinically applied basic research advances of the past 50 years. (laskerfoundation.org)
Western1
- We also describe a Western blot technique in which tive analysis of monkeypox outbreaks. (cdc.gov)
Method2
- Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. (portlandpress.com)
- Chen H, McCoy LF, Schleicher RL, Pfeiffer CM. Measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method external icon . (cdc.gov)
Production1
- Randomly collected or "spot" urine specimens were collected by clean-catch technique into sterile 250-mL polyethylene containers (from a large production lot, previously prescreened for trace element contamination). (cdc.gov)
Blood1
- Radioimmunoassay made it possible to screen the blood of donors and babies for diseases. (riverdalepress.com)
Control2
Diagnosis1
- [ 11 ] The diagnosis of Zollinger-Ellison syndrome is made on the basis of a combination of criteria, including the clinical presentation, gastrin radioimmunoassay findings, gastric acid secretory testing, and diagnostic imaging evaluation. (medscape.com)
Flow1
- At 0, 6 and 12 months, glomerular filtration rate (GFR) and renal plasma flow (RPF) were measured by the constant infusion technique, and UAE by radioimmunoassay. (unboundmedicine.com)
Concentration1
- 1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples. (portlandpress.com)
Level1
- Radioimmunoassay (RIA) technique reveals the level of progesterone at 30-70 pg/ml during the follicular phase, and 400-700 pg/ml in luteal phase. (vin.com)
Research1
- In 1995, visiting scientist at Glaxo Research Laboratories, Department of Pharmacology, Verona, Italy, studying the localisation of Stress Activated Protein Kinases (SAPKs) in the rat central nervous system with neuroanatomical techniques. (unibo.it)
Variety1
- Air samples were drawn through cellulose-ester and polycarbonate filters, and the filters were examined by a variety of microscopic techniques. (cdc.gov)
Plasma1
- Similar plasma levels and excretion values of angiopeptin were observed by using radioimmunoassay and radiolabeling techniques. (aspetjournals.org)
Multiple1
- A 2 x 6 months randomised cross-over study was performed using continuous subcutaneous insulin infusion (CSII) and multiple injection technique (MIT). (unboundmedicine.com)
Population1
- This technique yielded a population of T pallidum that failed to bind to fibronectin. (bmj.com)