Target protein engineering. Medical search. Frequent questions

The engineers addressed this problem by modifying proteins known as transcription activator-like effectors, or TALEs, which are able to turn off a wide range of genes. (dailycardinal.com)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

My Ph.D. projects centered on using computational structural biology tools to develop protein engineering methods for targeted therapeutic delivery, emphasizing delivering molecules to the brain. (caltech.edu)
First, utilizing computational structural biology techniques, I investigate the molecular mechanism that enables engineered adeno-associated viral (AAV) capsids to cross the blood-brain barrier (BBB). (caltech.edu)
Green fluorescent protein (GFP) is a widely used scaffold for protein-based targeted nanomedicines because of its high biocompatibility, biological neutrality and outstanding structural stability. (uab.cat)
Here we report a human nidogen-derived protein (HSNBT), rationally designed to mimic the structural and functional properties of GFP as a scaffold for nanomedicine. (uab.cat)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
RESULTS: Structural comparisons ofrepresentative members of already known protein families structurallyrelated to Bet v 1 with all entries of the Protein Data Bank yielded 47structures with non-identical sequences. (embl.de)
Structural diversity of leucine-rich repeat proteins. (embl.de)
To identify such inaccuracies, scoring functions have been developed which analyze different structural features of the protein models in order to derive a quality estimate. (unibas.ch)
Structural proteins are attached to microfilaments in the cytoskeleton which ensures stability of the cell. (phys.org)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

September 15, 2023 - Vaccine targets six flu strains. (nih.gov)
July 27, 2023 - Trial results show that engineered T cells can effectively target mesothelin, a protein found on many solid tumors. (nih.gov)
New York, May 23, 2023 (GLOBE NEWSWIRE) -- The Protein Engineering Market size is projected to surpass around USD 9,329 Million by 2032, and it is poised to reach a CAGR of 13.6% from 2023 to 2032. (globenewswire.com)
By Technology, the rational protein design segment has dominated the market, and it is growing at the highest CAGR over the forecast period 2023 to 2032. (globenewswire.com)
In June 2023, VRBPAC recommended the vaccine composition be updated to a 2023-2024 formulation to target the XBB.1.5 Omicron subvariant. (medscape.com)

To overcome this problem, Werewolf Therapeutics is utilizing Predator, a protein engineering technology, to create conditionally activated molecules called Indukines. (genengnews.com)

His current interests deal with the engineering of small molecules and proteins for sensing and targeting nucleic acid structures in vivo. (imperial.ac.uk)
The research in my lab is at the interface of chemistry and biology to investigate the molecular basis of diseases and discover novel therapeutic/technological approaches (e.g., ligands, biodrugs, nanodiscs, targeted nanovectors, lipid-nanoparticles for nucleic acid delivery, engineered viral cages and antibodies). (iit.it)
nanoBIOTECHNOLOGY , with applications that range from design and generation of bioactive 3D (nano-)biomaterials (e.g., nanodiscs, targeted nanovectors, lipid-nanoparticles for nucleic acid delivery, engineered viral cages and antibodies) to biodrugs and cascade biocatalysts in sustainable technology. (iit.it)
Once the target nucleic acids are recognized by the DNA probes, the pore-attached DNA nanolock caused remarkable impedance amplification by blocking the nanopores. (bvsalud.org)
Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. (bvsalud.org)
Current work in the group includes studies of platinum-, gold- and ruthenium-based anticancer active drugs and their influence on nucleic acid structure, transcription and protein translation. (lu.se)

The resulting construct T22-HSNBT-H6, is a humanized CXCR4-targeted nanoparticle that selectively delivers conjugated genotoxic Floxuridine into cancer CXCR4+ cells. (uab.cat)
Some of the applications that have been tested using affimers include cell imaging, super resolution microscopy, protein function modulation, magnetic nanoparticle formation, and MRI reagent development. (news-medical.net)

The ESK1 monoclonal antibody was engineered to recognize WT1 peptides brought to the surface of cancer cells. (mskcc.org)
The new monoclonal antibody, called ESK1, targets a protein that is associated with many types of cancer and is of great interest to cancer researchers. (mskcc.org)
The antibody-like proteins, called OptoBinders, allow researchers to rapidly control processes inside and outside of cells by directing their localization, with potential applications including protein purification, the improved production of biofuels, and new types of targeted cancer therapies. (scienceblog.com)
Another company reported that it is testing a chimeric PD-1 antibody/IL-2 fusion protein that recruits killer immune cells. (genengnews.com)
Did you know that ionic charge can affect antibody-target binding? (ezyspot.com)
Learn how Twist Bioscience helped a leading pharmaceutical company precisely interrogate key residues involved in both target recognition and antibody charge by synthesizing combinatorial variant libraries. (ezyspot.com)
Learn how Twist Bioscience helped relieve the biologics bottleneck in the antibody discovery pipeline with a gene to protein workflow that results in antibodies ready for screening. (ezyspot.com)
By Protein Type, the monoclonal antibody segment accounted for the largest revenue share. (globenewswire.com)
Genetically engineered antibody MIMETIC PROTEINS, derived from ANKYRIN PROTEINS. (bvsalud.org)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. (bvsalud.org)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In this system, large combinatorial libraries of macrocyclic molecules are biosynthesized in Escherichia coli cells and simultaneously screened for their ability to rescue pathogenic protein misfolding and aggregation using a flow cytometric assay. (nature.com)
Monoclonal antibodies are molecules that can be engineered to target specific proteins on cancer cells. (mskcc.org)
The team reported that one type of OptoBinder changed its affinity for its target molecules up to 330-fold when shifted from dark to blue light conditions, while others showed a five-fold difference in binding affinity - all of which could be useful to researchers seeking to understand and engineer the behaviors of cells. (scienceblog.com)
Because protein molecules are highly dynamic, constant chaperone surveillance is required to ensure protein homeostasis (proteostasis). (nature.com)
They target specific molecules believed to be involved in axial SpA. (nass.co.uk)
They are made up of genetically engineered proteins and are very large, complex molecules. (nass.co.uk)

The main interest of our group is the development of methods and algorithms for molecular modeling and simulations of three-dimensional protein structures and their interactions. (unibas.ch)
Ultimately, the quality of a model determines its usefulness for different biomedical applications such as planning mutagenesis experiments for functional analyses or studying protein-ligand interactions, e.g. in structure based drug design. (unibas.ch)
We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)
Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
Protein-based plastics are particularly interesting because their precisely controlled sequences can give rise to complex properties based on the synergy of diverse intermolecular interactions. (techconnectworld.com)
Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. (bvsalud.org)
Recently, with the in-depth research of aptamer-protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. (bvsalud.org)
There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer-protein interactions. (bvsalud.org)
Herein, we comprehensively introduce the methods for regulating aptamer-protein interactions and elaborate on the detection techniques for analyzing aptamer-protein interactions. (bvsalud.org)
Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer-protein interactions for detecting biomolecules. (bvsalud.org)
A particular focus is development of new proteomics techniques to investigate protein interactions between host and pathogen and to investigate systemic proteome changes during sepsis. (lu.se)
His research aims to elucidate molecular mechanisms underlying bacterial interactions with human cells and to use that information to identify targets for treatment and diagnostics of bacterial infections. (lu.se)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

Whole-body PD-L1 expression PET imaging may potentially allow for a better prediction of response to programmed cell death protein-1-targeted therapies. (snmjournals.org)
Because these therapies are proteins, they do not work as tablets and have to be given as injections into the skin. (nass.co.uk)

The responsibility of Protein Structure and Bioinformatics Group, Lund University is limited to applying the best efforts in providing and publishing good programs and data. (lu.se)
Thomas Laurell is Professor in Medical and Chemical microsensors and heads the division of Nanobiotechnology at the Department of Biomedical Engineering, Lund University. (lu.se)
Below you can see some examples of the infrastructure for proteins, available for researchers at Lund University. (lu.se)

Because of their large size, monoclonal antibodies can target only proteins located on the outside of cancer cells. (mskcc.org)
Blocking the interaction of the immune checkpoint molecule programmed cell death protein-1 and its ligand, PD-L1, using specific antibodies has been a major breakthrough for immune oncology. (snmjournals.org)
Crucially, OptoBinders can target proteins that are naturally present in cells, and their binding is easily reversible by changing light conditions - "a new capability that is not available to normal antibodies," said co-author José Avalos , an assistant professor of chemical and biological engineering and the Andlinger Center for Energy and the Environment . (scienceblog.com)
Antibodies have been the most popular method for studying protein expression and function for the past decades, but have been dogged by lack of validation and the poor cost-effectiveness of constantly generating new antibodies. (news-medical.net)
Affimers stem from the drive to generate alternative methods of studying protein binding and function, which can complement information provided by antibodies. (news-medical.net)
Affimers with enhanced specificity for tubulin have been used in super resolution microscopy with high success, and can target regions of the tubulin structure that antibodies are typically restricted from. (news-medical.net)
They offer advantages over antibodies because of their highly specific target PROTEIN BINDING with high affinity and specificity. (bvsalud.org)
In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
The scFv antibodies are engineered with a FLAG tag to allow for secondary detection using standard immunohistochemistry methods. (cdc.gov)

Stability of biomolecules, especially of proteins, is of great interest and significance. (lu.se)
Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)

Both joined the Princeton faculty in 2015, and soon began working together on new ways to apply optogenetics - a set of techniques that introduce genes encoding light-responsive proteins to control cells' behaviors. (scienceblog.com)
A couple of highly interesting target genes have been identified, partially characterized and filed for patenting. (tugraz.at)
We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)
University engineers hope their discovery on switching off genes can have broader applications. (dailycardinal.com)
A group of UW-Madison engineers has discovered how to turn on and off specific genes within bacteria, according to a university press release. (dailycardinal.com)
We were frustrated because synthetic biology is littered with examples of artificial factors that can turn on and turn off gene expression under different conditions, but they only work for certain genes," said Brian Pfleger, a UW-Madison associate professor of chemical and biological engineering. (dailycardinal.com)
New metabolic engineering approaches have recently been developed to modulate carotenoid content, including the employment of CRISPR technologies for single-base editing and the integration of exogenous genes into specific "safe harbors" in the genome. (frontiersin.org)
The objective of this project is to engineer defines factors that are potent in transactivating downstream target genes for the generation of induced pluripotent stem cells (iPSCs). (ca.gov)
The binding of virally expressed defined factors to the downstream target genes is not a limiting factor in the iPSC induction. (ca.gov)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

We hope that this is going to be the beginning of the next era of optogenetics, opening the door to light-sensitive proteins that can interface with virtually any protein in biology, either inside or outside of cells," said Toettcher, the James A. Elkins, Jr. '41 Preceptor in Molecular Biology. (scienceblog.com)
Hartl, F. U. Molecular chaperones in cellular protein folding. (nature.com)
We thus used molecular approaches to engineer the OCT4 protein factor in order to enhance the remodeling of intra-chromosomal interaction. (ca.gov)
Proteins, 19, 277-290 (1994) +} {+ reference: Stein EG, Rice LM, Brunger AT, Torsion angle molecular dynamics: a new efficient tool for NMR structure calculation. (lu.se)
With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)

Biologics are genetically engineered proteins created from living organisms that mimic normal biological functions. (healthline.com)
Biologic drugs or "biologics" are genetically engineered from proteins derived from living cells or tissues. (nationaleczema.org)

NEW YORK (GenomeWeb) - Duke University researchers reported today in a new study that they were able to increase the specificity of CRISPR-based gene editing by several orders of magnitude by engineering a hairpin secondary structure onto the spacer region of single guide RNAs (hp-sgRNAs) and combining them with various CRISPR effectors. (genomeweb.com)
Specifically, as they wanted to analyze this interaction in human cells, they decided to utilize nuclease-inactive dCas9-based transcriptional activators where endogenous gene activation can serve as a sensitive measure of dCas9 binding to target DNA. (genomeweb.com)
We used a spacer that targets the endogenous promoter of IL1RN, a gene we have previously activated with high efficiency," the authors wrote. (genomeweb.com)
Transfecting sgRNA variants and a dCas9-P300 transactivator into human cells, we observed that hp-sgRNAs can tune gene activation at the target locus, suggesting modulation of dCas9 binding. (genomeweb.com)
The researchers also found that hp-sgRNAs increased the specificity of gene editing with SaCas9 and AsCas12a proteins as well. (genomeweb.com)
The engineers manipulated the TALEs so that the proteins repressed gene expression, as opposed to promoting it. (dailycardinal.com)
Protein engineering refers to the process in which a researcher transforms a protein sequence through insertion, substitution, or deletion of nucleotides in the encoding gene, aiming to obtain a modified protein that is more appropriate for a particular application or purpose than an unmodified protein. (globenewswire.com)
Currently, FLIP encompasses experimental data across adeno-associated virus stability for gene therapy, protein domain B1 stability and immunoglobulin binding, and thermostability from multiple protein families. (nips.cc)
Typically, nucleotide sequences encoding the protein/peptide library are fused to a gene encoding a phage coat protein thus allowing them to be displayed on the phage exterior. (cdc.gov)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

Statement of significance: Targeted nanomedicine seeks for humanized and biologically neutral protein carriers as alternative of widely used but immunogenic exogenous protein scaffolds such as green fluorescent protein (GFP). (uab.cat)
As a type of affinity reagent, affimers are biophysically stable scaffolds of proteins that can be reused. (news-medical.net)

With 4D printing, engineers have created tiny needles that mimic parasites that attach to tissues and could replace hypodermic needles, according to a new study. (sciencedaily.com)

In a pair of papers published Aug. 13 in Nature Communications, the researchers describe the creation of OptoBinders that can specifically latch onto a variety of proteins both inside and outside of cells. (scienceblog.com)
Biological membranes consist of a phospholipid bilayer and a variety of proteins that accomplish vital biological functions. (phys.org)

A distance-based phylogenetic treeyielded a classification into 11 subfamilies, nine exclusively containingplant sequences and two subfamilies of bacterial proteins. (embl.de)
The superfamily of leucine-rich repeat proteins can be subdivided into at least six subfamilies, characterised by different lengths and consensus sequences of the repeats. (embl.de)
At least six families of LRR proteins, characterised by different lengths and consensus sequences of the repeats, have been identified. (embl.de)
Template-based protein modeling techniques exploit the evolutionary relationship between a target protein and templates with known experimental structures, based on the observation that evolutionarily related sequences generally have similar 3D structures. (unibas.ch)
The " Continuous Automated Model EvaluatiOn" (CAMEO) project aims to complement CASP and provide a fully automated blind assessment for prediction servers only based on weekly pre-released sequences of the Protein Data Bank PDB. (unibas.ch)
Because TALEs are able to recognize specific letters in DNA, they were the perfect tool to target particular DNA sequences. (dailycardinal.com)

Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)

We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)

Most proteins must fold into defined three-dimensional structures to gain functional activity. (nature.com)
In this study, we aimed to identify and classify all Betv 1-related structures from the Protein Data Bank and all Bet v 1-relatedsequences from the Uniprot database. (embl.de)
The approach used for the prediction of the leucine-rich repeat protein structures can be applied to other proteins containing internal repeats of about 20 to 30 residue in length. (embl.de)
One of the major limitations for using structure-based methods in biomedical research is the limited availability of experimentally determined protein structures. (unibas.ch)
Comparative or homology modeling, which uses experimentally elucidated structures of related protein family members as templates, is currently the most accurate and reliable approach to model the structure of the protein of interest. (unibas.ch)
QMEANBrane further extends the approach to membrane protein structures, which play crucial roles in many biological processes and are important drug targets. (unibas.ch)
Jan. 6, 2022 Protein assembly is essential for the formation of ordered biological structures, but imagine engineering one! (sciencedaily.com)
Our research aims to develop new platforms for producing silk-inspired polymers with well-defined chemical structures and targeted material properties. (techconnectworld.com)
reference: Nilges M, Gronenborn AM, Brunger AT, Clore GM, Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints: application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2. (lu.se)
Protein Engineering 2, 27-38, (1988) +} {+ reference: Nilges M, Clore GM, Gronenborn AM, Determination of three-dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms. (lu.se)

His group's research centres on the chemical understanding and exploitation of biomolecular function (Synthetic Biology, Chemical Biology and Chemical Medicine), with an emphasis on carbohydrates and proteins. (wikipedia.org)
These have enabled associated mechanistic details of protein and sugar biology to be elucidated and exploited for biotechnological applications. (wikipedia.org)
Prediction of the 3D structure of a protein from its amino acid sequence remains a fundamental scientific problem, and it is considered as one of the grand challenges in computational biology. (unibas.ch)

Our conceptual approach is to identify protein targets involved in bacterial sensory or metabolic pathways important in the infectious process and to describe their function both at the atomic level and at the host-microbe interaction scale. (leloir.org.ar)
We will additionally discuss recent findings regarding the metabolic challenges associated with producing recombinant silk in bacteria, and we will present our work in engineering a new E. coli strain with significantly improved capacity for synthesizing recombinant silk. (techconnectworld.com)

Therefore, we study enzyme characteristics by classical protein analytics and devise novel enzyme assays. (tugraz.at)
For bacterial in vivo characterization, we evaluate the biological relevance of our targets in the host-microbe interaction: we use genome site directed mutagenesis, homologous and heterologous complementation with wild-type and mutant versions of the proteins, classical microbiological determination assays (i.e. biofilm formation, EPS content, flagellar apparatus production, motility, survival to stresses, growth, etc). (leloir.org.ar)

This unnatural amino acid can be site-specifically incorporated into proteins expressed in both Escherichia coli and eukaryotic cells by genetic modifications. (chemistryviews.org)
The protein engineering market is growing speedily and creating numerous growth opportunities due to the increasing number of engineered proteins and amino acids worldwide. (globenewswire.com)

In order to increase the specificity of class 2 CRISPR systems and reduce off-target effects, the researchers began with the general strategy of reducing the energetics of DNA interrogation by the Cas9-sgRNA complex. (genomeweb.com)
In order to engineer hp-sgRNAs, the researchers began by extending the protospacer-adjacent motif (PAM)-distal end of the spacer. (genomeweb.com)
The researchers generated a variety of hp-sgRNAs, and measured indel frequency at on-target and off-target sites for each spacer. (genomeweb.com)
At the off-target sites, hp-sgRNAs showed decreases of 91 percent, 79 percent, and 67 percent [for SpCas9, SaCas9 and AsCas12a, respectively] relative to WT-sgRNAs, compared with decreases of 88 percent, 38 percent, and 0 percent for the ns-sgRNAs," the researchers wrote. (genomeweb.com)
This is exactly what researchers have now accomplished with protein needles. (sciencedaily.com)
In about a fifth of the cases of Parkinson's disease, look to a small, malfunctioning protein in the lysosome as a risk factor, say University of Michigan researchers. (phys.org)

The chosen coding regions are then cloned into an expression vector in Escherichia coli , and the recombinant protein was purified over the course of a week. (news-medical.net)

The taxonomicdistribution of these families extracted from the Pfam protein familydatabase showed that members of the polyketide cyclase family and theactivator of Hsp90 ATPase homologue 1 family were distributed among allthree superkingdoms, while members of some bacterial families wereconfined to a small number of species. (embl.de)

I develop a pipeline to model the vast and dynamic complex between engineered AAV capsids and their BBB receptors. (caltech.edu)
New research on protein receptors could lead to breakthroughs in improving drug delivery. (umn.edu)

Second, I describe the development of Automated Pairwise Peptide-Receptor AnalysIs for Screening Engineered proteins (APPRAISE). (caltech.edu)
This computational pipeline predicts the receptor binding propensity of engineered proteins based on competitive modeling and physics-grounded analysis. (caltech.edu)
I show that APPRAISE is capable of distinguishing between receptor-dependent and receptor-independent adeno-associated viral vectors and ranking various engineered proteins, such as miniproteins binding to the SARS-CoV-2 spike and nanobodies binding to a G-protein-coupled receptor. (caltech.edu)
A melatonin indicator was then created by integrating the repurposed receptor with a fluorescent protein. (caltech.edu)
Finally, some are engineering chimeric antigen receptor (CAR) T-cell systems that attack solid tumors and overcome the harsh barriers of the tumor microenvironment (TME). (genengnews.com)
By finding biomarkers, affimers have been applied to cancer studies, where they have been used to target a protein in tumor vessel formation called the VEGF receptor. (news-medical.net)
Membrane receptor proteins serve as connection between the cell's internal and external environments. (phys.org)

This scaffold is the basis of a humanized nanoconjugate, where GFP, from the well-characterized protein T22-GFP-H6, has been substituted by the nidogen-derived GFP-like HSNBT protein. (uab.cat)
Therefore, the newly developed GFP-like protein scaffold appears as an ideal candidate for the development of humanized protein nanomaterials and successfully supports the tumor-targeted nanoscale drug T22-HSNBT-H6-FdU. (uab.cat)
The stefin A scaffold is engineered from human stefin A protein, whereas Adhiron is synthetic. (news-medical.net)

Protein misfolding and aggregation are common pathological features of several human diseases, including Alzheimer's disease and type 2 diabetes. (nature.com)

The multi-compartment protein p32 is overexpressed and present at cell surfaces in a variety of tumors, including TNBC, specifically in the malignant cells and endothelial cells, and in macrophages localized in hypoxic areas of the tumor. (oncotarget.com)
This has been demonstrated in T22-HSNBT-H6-FdU, a humanized CXCR4-targeted protein nanoconjugate able to selectively deliver its genotoxic load into cancer cells. (uab.cat)
The ability to let go [of a target protein] is actually very valuable for many applications," said Avalos, including engineering cells' metabolisms, purifying proteins or potentially making biotherapeutics. (scienceblog.com)
Monobodies, on the other hand, are engineered pieces of human fibronectin, a large protein that forms part of the matrix between cells. (scienceblog.com)
For example, SH2 proteins expressed in mammalian cells are challenging to extract. (news-medical.net)
Cell recognition proteins allow cells to identify each other and interact. (phys.org)
Using Ce6 as a drug model, these C18-aptamer micelles exhibit efficient tumor-targeting and -binding ability, facilitating the entry of Ce6 into targeted cells for photodynamic therapy. (bvsalud.org)
The ideal drug would target only cancer cells and have no adverse effects on normal cells. (msdmanuals.com)
Current research targets ultrasonic separation and enrichment of cells, bacteria, virus and extracellular vesicles aimed at providing new technology for faster sepsis diagnostics. (lu.se)

Herein we used polyethylene glycol-polycaprolactone polymersomes that were affinity targeted with the p32-binding tumor penetrating peptide LinTT1 (AKRGARSTA) for imaging of TNBC lesions. (oncotarget.com)
In a TNBC model in mice, systemic LinTT1-targeted polymersomes accumulated in early tumor lesions more than twice as efficiently as untargeted polymersomes with up to 20% ID/cc at 24 h after administration. (oncotarget.com)
Confocal imaging of tumor tissue sections revealed a high degree of vascular exit and stromal penetration of LinTT1-targeted polymersomes and co-localization with tumor-associated macrophages. (oncotarget.com)
Our studies show that systemic LinTT1-targeted polymersomes can be potentially used for precision-guided tumor imaging and treatment of TNBC. (oncotarget.com)
The company asserts that its Indukines can enhance tumor-specific killing while minimizing undesirable off-target effects. (genengnews.com)

Using engineered microbes, protein-based plastics can be made from renewable resources. (techconnectworld.com)

For that aim, we functionally characterize these targets in vitro and in vivo. (leloir.org.ar)

The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. (mdpi.com)
We think our 4D-printed microneedle array will allow for more robust and sustained use of minimally invasive, pain-free and easy-to-use microneedles for delivering drugs, healing wounds, biosensing and other soft tissue applications," said senior author Howon Lee, an assistant professor in the Department of Mechanical and Aerospace Engineering in the School of Engineering at Rutgers University-New Brunswick. (sciencedaily.com)
Inspired by these examples, Rutgers engineers developed a microneedle that interlocks with tissue when inserted, enhancing adhesion. (sciencedaily.com)
These innovations have potential applications in environmental sustainability (e.g. biodegradable plastics for a circular economy) and in healthcare (e.g. materials for tissue engineering and drug release). (techconnectworld.com)

The global protein engineering market size accounted for USD 2,691 Million in 2022. (globenewswire.com)

More than half of all proteins interact with membranes. (phys.org)

PROTEIN BIOTECHNOLOGY , from design of engineered proteins to the structure of membrane protein targets for ligand discovery and biomolecular medicine. (iit.it)

Third, I show an example to engineer a genetically encoded transmitter indicator (GETI), which may eventually be a cargo delivered to the brain. (caltech.edu)
An Oxidative Bioconjugation Strategy Targeted to a Genetically Encoded 5-hydroxytryptophan , Partha Sarathi Addy, James S. Italia, Abhishek Chatterjee, ChemBioChem 2018 . (chemistryviews.org)

But in the cellular environment, newly synthesized proteins are at great risk of aberrant folding and aggregation, potentially forming toxic species. (nature.com)
Skach, W. R. Cellular mechanisms of membrane protein folding. (nature.com)

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl.de)

The Critical Assessment of Structure Prediction ( CASP ) experiment has for the last 25 years assessed the progress in the field of protein structure modeling. (unibas.ch)
To this end, every two years, about 100 blind prediction targets are carefully evaluated by human experts. (unibas.ch)
In this work, we introduce Fitness Landscape Inference for Proteins (FLIP), a benchmark for function prediction to encourage rapid scoring of representation learning for protein engineering. (nips.cc)

Proteins in lipid membranes are one of the fundamental building blocks of biological functionality. (phys.org)

The market is growing positively due to the rising utilization of protein drugs over non-protein ones. (globenewswire.com)
Furthermore, the utilization of engineered microorganisms allowed efficient mass production of carotenoids, making it convenient for industrial practices. (frontiersin.org)

This work reports for the first time the rational engineering of a human homolog of the GFP based in the human nidogen (named HSNBT) that shows full potential to be used in humanized protein-based targeted nanomedicines. (uab.cat)
Rational and evolutionary enzyme engineering approaches allow us to make novel enzymes or enzyme families fit for biocatalytic applications, as isolated enzymes or in whole-cell applications. (tugraz.at)

Bartlett, A. I. & Radford, S. E. An expanding arsenal of experimental methods yields an explosion of insights into protein folding mechanisms. (nature.com)
DNA transcription, DNA damage repair mechanisms), focusing on the identification of new therapeutic targets and of disease-related biomarkers. (imperial.ac.uk)

The site-specific chemical modification of proteins is a powerful method to understand and engineer protein function. (chemistryviews.org)

They are designed to target specific parts of the immune system that contribute to chronic inflammatory diseases such as atopic dermatitis. (nationaleczema.org)

Furthermore, the difference in the packing explains why the repeats from different subfamilies never occur simultaneously in the same protein. (embl.de)
Eleven-residue segments of the LRRs (LxxLxLxxN/CxL), corresponding to the beta-strand and adjacent loop regions, are conserved in LRR proteins, whereas the remaining parts of the repeats (herein termed variable) may be very different. (embl.de)

In addition, determination of the Bet v 1structure revealed the existence of a large superfamily of structurallyrelated proteins. (embl.de)

The SWISS-MODEL expert system developed by our group is a fully automated web-based workbench, which greatly facilitates the process of computing of protein structure homology models. (unibas.ch)
They hypothesized that engineering the sgRNA might serve to regulate diverse CRISPR systems, and they specifically engineered an RNA secondary structure onto the spacer by extending a designed hairpin on the 5' end of the sgRNA. (genomeweb.com)
We hypothesized that by adjusting the strength of the secondary structure, R-loop formation could proceed to completion at the on-target site but could be impeded at off-target sites, which have reduced energetics due to RNA-DNA mispairing. (genomeweb.com)
Existing benchmarks like CASP or CAFA assess structure and function predictions of proteins, respectively, yet they do not target metrics relevant for protein engineering. (nips.cc)
In press (1998) +} {+ reference: Garrett DS, Kuszewski J, Hancock TJ, Lodi PJ, Vuister GW, Gronenborn AM, Clore GM, The impact of direct refinement against three-bond HN-C alpha H coupling constants on protein structure determination by NMR. (lu.se)
B, 104(1), 99-103, (1994) May +} {+ reference: Kuszewski J, Qin J, Gronenborn AM, Clore GM, The impact of direct refinement against 13C alpha and 13C beta chemical shifts on protein structure determination by NMR. (lu.se)
B, 106(1), 92-6, (1995) Jan +} {+ reference: Kuszewski J, Gronenborn AM, Clore GM, The impact of direct refinement against proton chemical shifts on protein structure determination by NMR. (lu.se)

There are more drugs currently in development and going through clinical trials which target these and other pathways, which should become available in the future if they are shown to work safely. (nass.co.uk)
Increasing frequencies of chronic diseases would fuel the demand for protein engineering in order to develop effective protein drugs for the treatment of chronic diseases, and this will lead to market growth. (globenewswire.com)
Nanoengineers at the University of California San Diego have developed immune cell-mimicking nanoparticles that target inflammation in the lungs and deliver drugs directly where they're needed. (phys.org)

Research in the Davis laboratory has been funded by the Engineering and Physical Sciences Research Council, the Biotechnology and Biological Sciences Research Council, the Medical Research Council, UCB-Celltech, AstraZeneca, the European Union, GlaxoSmithKline, Cancer Research UK, the Wellcome Trust and the Royal Society. (wikipedia.org)

Sequence analyses of LRR proteins suggested the existence of several different subfamilies of LRRs. (embl.de)

Anatomy2

Organisms5

Chemicals and Drugs51

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes53

Disciplines and Occupations9

Technology, Industry, Agriculture17

Information Science8

Health Care1

Transcription activator-like effectors2

Structural10

20235

Therapeutics1

Nucleic6

Nanoparticle2

Antibody9

Ability to interact with proteins2

Molecules7

Interactions13

Therapies2

Lund University3

Antibodies9

Biomolecules2

Genes14

Molecular5

Biologics2

Gene10

Scaffolds2

Mimic1

Variety of proteins2

Sequences6

Membrane proteins1

Endogenous2

Structures10

Biology3

Metabolic2

Assays2

Amino2

Researchers6

Recombinant protein1

Bacterial1

Receptors2

Receptor7

Scaffold3

Misfolding and aggregation1

Cells9

Tumor5

Microbes1

Vivo1

Tissue4

20221

Interact1

Ligand1

Genetically encoded2

Cellular2

Genomic1

Prediction3

Lipid1

Utilization2

Rational2

Mechanisms2

Understand and engineer1

Immune system1

Repeats2

Revealed the existence1

Structure7

Drugs3

Biotechnology1

Analyses1