VinculinKinasePaxillinEzrinHost cytoskeletalPlasma membraneAdhesionCytoskeletonZyxinInclude actinTGB2 and TGB3Nascent adhesionsRegulateGeneFERMFilamentsRegulatorBindSignal transductionMediateLocalizationAssociateStructuresAdaptor proteinAttachmentInteractionsStructuralMeSHViralOrganizationResiduesInteractsChemotaxisTransmembraneSuperFamilyPhosphorylationProliferationKinasesTyrosine1995PhosphataseMembrane associationMRNARolesCellFamilyMechanicalMetastatic
Vinculin13
- Some components have conformations that are tension sensitive (like talin, vinculin, and p130Cas, not shown here). (cellmigration.org)
- Although focal adhesions are similar, in terms of molecular composition, to nascent adhesions and focal complexes, several adhesion proteins appear to change as focal adhesions evolve in response to tension, e.g., zyxin and vinculin . (cellmigration.org)
- Vinculin controls talin engagement with the actomyosin machinery. (univ-paris5.fr)
- Deletion of the cell adhesion adaptor protein vinculin disturbs the localization of GFAP in Bergmann glial cells. (univ-paris5.fr)
- RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover. (univ-paris5.fr)
- Vinculin regulates the recruitment and release of core focal adhesion proteins in a force-dependent manner. (univ-paris5.fr)
- The talin-vinculin axis is a key mechanosensing component of cellular focal adhesions. (bvsalud.org)
- How talin and vinculin respond to forces and regulate one another remains unclear. (bvsalud.org)
- By combining single-molecule magnetic tweezers experiments, Molecular Dynamics simulations, actin-bundling assays, and adhesion assembly experiments in live cells, we here describe a two-ways allosteric network within vinculin as a regulator of the talin-vinculin interaction. (bvsalud.org)
- We directly observe a maturation process of vinculin upon talin binding, which reinforces the binding to talin at a rate of 0.03 s-1. (bvsalud.org)
- This allosteric transition can compete with force-induced dissociation of vinculin from talin only at forces up to 10 pN. (bvsalud.org)
- Hence, the allosteric switch confines talin-vinculin interactions and focal adhesion build-up to intermediate force levels. (bvsalud.org)
- actinin, talin and vinculin) transcellularly results in IgG, IgM, and IgA antibody production against bacterial cytotoxins along with cytoskeletal proteins and may even lead to an increased risk for autoimmunity due to molecular mimicry. (lifestylematrix.com)
Kinase13
- PTK2 protein tyrosine kinase 2 (PTK2), also known as focal adhesion kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. (wikipedia.org)
- PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion (how cells stick to each other and their surroundings) and spreading processes (how cells move around). (wikipedia.org)
- The PTK2 gene encodes a cytosolic protein tyrosine kinase that is found concentrated in the focal adhesions that form among cells attaching to extracellular matrix constituents. (wikipedia.org)
- FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. (wikipedia.org)
- Non-receptor tyrosine-protein kinase TYK2. (embl.de)
- Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
- Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)
- 1997). Furthermore, it's possible that calpain cleavage of proteins kinase C (PKC)1 (Melloni et al. (thetechnoant.info)
- The human being FAK (also called PTK2, protein tyrosine kinase 2) gene continues to be mapped to chromosome 8 (15, 16). (cahrr.org)
- Kinase site is involved with binding with FIP200 protein. (cahrr.org)
- This cascade causes following cytoskeletal adjustments and activation of RAS-MAPK (mitogen-activated protein kinase) signaling pathways (26, 28). (cahrr.org)
- Phosphorylated Y397 FAK can recruit another essential signaling protein, p85 PI3-kinase (phosphoinositide 3-kinase), development factor receptor destined protein Grb 7, phospholipase Cgamma(PLCgamma) and. (cahrr.org)
- The integrin tails have no intrinsic kinase activity but rather serve as a site for the docking of various kinases and related adaptor proteins that comprise focal adhesions. (thno.org)
Paxillin1
- Y861 and Y925 (19, 26), also to phosphorylation of FAK binding proteins after that, such as for example paxillin and Cas (27). (cahrr.org)
Ezrin6
- Ezrin, moesin, and radixin are highly related proteins (ERM protein family), but the other proteins in which the FERM domain is found do not share any region of similarity outside of this domain. (embl.de)
- These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)
- The ERM proteins ezrin, however, not radixin or moesin, is certainly markedly increased because of calpain inhibition. (thetechnoant.info)
- Lately, interest continues to be centered on the implications of calpain cleavage from the membrane-bridging proteins ezrin in gastric parietal cells (Yao et al. (thetechnoant.info)
- 1997). Lately, it's been recommended that calpain facilitates cytoskeletal reorganization during cell motility by cleaving ezrin substances (Shuster and Herman, 1995) that type a bridge between your membrane and actin filaments (Algrain et al. (thetechnoant.info)
- Immunofluorescence microscopy has shown that the area of host cell in proximity to the bacterium contains polymerized actin, α-actinin, talin, and ezrin ( 2 ). (cdc.gov)
Host cytoskeletal2
- Intracellular transport of plant viruses during infection operates via unique interactions between viral proteins and selected host cytoskeletal and membrane elements. (ppjonline.org)
- The EspA organelle is used to transfer the translocated intimin receptor (Tir), which is then inserted into the host cell membrane, where it binds to intimin, a bacterial outer membrane protein ( 9 , 10 ) and triggers the host cytoskeletal events that lead to attaching/effacing lesion formation. (cdc.gov)
Plasma membrane2
- FERM domains are found in a number of cytoskeletal-associated proteins that associate with various proteins at the interface between the plasma membrane and the cytoskeleton. (embl.de)
- Moesin, which is probably involved in binding major cytoskeletal structures to the plasma membrane. (embl.de)
Adhesion13
- FAK is a protein of 125 kD recruited as a participant in focal adhesion dynamics between cells, and has a role in motility and cell survival. (wikipedia.org)
- Release of this auto-inhibitory interaction has been shown to occur within focal adhesions-but not in the cytoplasm-and therefore is thought to require interaction with focal adhesion proteins, potentially as a result of mechanical forces transmitted through the focal adhesion. (wikipedia.org)
- Focal-adhesion kinases (FAKs), cytoplasmic protein tyrosine kinases involved in signalling through integrins. (embl.de)
- Adhesion complexes (i.e., focal adhesions and focal complexes) and cell-cell contacts are specialized structures that harbor a large number of cytoskeletal proteins and one of the highest concentrations of signaling molecules in cells ( Ruoslahti and Obrink, 1996 ). (rupress.org)
- All other tissues appeared normal, suggesting that, in most cases, tensin's diverse functions are redundant and may be compensated for by other focal adhesion proteins. (ppbhg.org)
- At the core of the focal adhesion is a cluster of activated integrin heterodimers, which are transmembrane signaling proteins that attach cells to their ligands, i.e., extracellular matrix (ECM)1 (Hynes, 1992). (ppbhg.org)
- 1995). Of the myriad of focal adhesion proteins, tensin is particularly interesting because it has the ability to bind to actin microfilaments at multiple sites, enabling tensin both to cap the growing (barbed) ends of actin filaments and to cross-link actin filaments (Lo et al. (ppbhg.org)
- We prepared Titanium (Ti) and Ce-TZP/Al 2 O 3 disks with different surface roughness, namely, mirrored and machined surfaces, on which HGF-1 were cultured, and then the biological parameters of the cultured cells, including cell morphology and gene expression of focal adhesion proteins were assessed. (alliedacademies.org)
- A lager expansion of the cell area accompanied by a greater development of actin cytoskeleton and talin1, a greater adhesion strength of CTEs to each disk, and higher gene expression level of focal adhesion protein were observed with the mirror-polished surface than the mechanically polished surface both Ti and Ce-TZP/Al 2 O 3 disks. (alliedacademies.org)
- In chapter 3, I show that in B16F1 melanoma cells, the expression of three cancer signature genes, Cyr61, MUC18 and TRPM1, is strongly regulated by cell-ECM adhesion, independently of cytoskeletal tension. (ubc.ca)
- Integrins are proteins that function mechanically, by attaching the cell cytoskeleton to the extracellular matrix (ECM), and biochemically, by sensing whether adhesion has occurred. (mechanobio.info)
- Integrins function as adhesion receptors for extracellular ligands and transduce biochemical signals into the cell, through downstream effector proteins. (mechanobio.info)
- A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON. (bvsalud.org)
Cytoskeleton10
- FAK is typically located at structures known as focal adhesions, which are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. (wikipedia.org)
- Protein-tyrosine phosphatases PTPN3 and PTPN4, enzyme that appear to act at junctions between the membrane and the cytoskeleton. (embl.de)
- For cytoskeleton-membrane cross-linking, the dormant molecules becomes activated and the FERM domain attaches to the membrane by binding specific membrane proteins, while the last 34 residues of the tail bind actin filaments. (embl.de)
- These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. (biomedcentral.com)
- en] The cytoskeleton is an important system in health and disease. (uni.lu)
- Testin is one of these cytoskeletal proteins described in important processes involving the actin cytoskeleton such as cell migration and cell spreading. (uni.lu)
- Testin is involved in the regulation of the actin cytoskeleton through being part of a protein complex with amongst other zyxin and talin, also able to concentrate to the midbody. (uni.lu)
- Adhesome complexes contain signaling proteins as well as proteins that link integrins to the cytoskeleton, thereby transducing extracellular forces into the cell. (ubc.ca)
- The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
- Rho GTPases represent a family of small GTP-binding proteins involved in cell cytoskeleton organization, migration, transcription, and proliferation. (biomedcentral.com)
Zyxin1
Include actin1
- Rho GTPase effectors are a large group of proteins and include actin nucleation promoting molecules, adaptors, as well as kinases. (biomedcentral.com)
TGB2 and TGB31
- The cell-to-cell transport of many plant viruses through plasmodesmata requires viral movement proteins (MPs) encoded by a 'triple gene block' (TGB) and termed TGB1, TGB2 and TGB3. (microbiologyresearch.org)
Nascent adhesions1
- Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. (gliome.org)
Regulate2
- Lately, it's been recommended that calpain, the ubiquitous Ca2+-turned on protease, may regulate cell motility by cleaving actin-associated cytoskeletal protein within a site-specific way (Beckerle et al. (thetechnoant.info)
- In chapter 6, I show that the two isoforms of talin, talin 1 and talin 2, differentially regulate expression of the three cancer signature genes and have different effects on B16F1 spreading, migration and in vivo tumor growth. (ubc.ca)
Gene5
- Ribbon boosts ribosomal protein gene expression to coordinate organ form and function. (uchicago.edu)
- To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. (ppjonline.org)
- 3. FAK GENE Framework First, FAK cDNA encoding 125 kDa protein was isolated from poultry embryo cells (1). (cahrr.org)
- Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)
- In chapter 4, I used global transcriptome profiling to compare gene expression changes caused by increased ECM ligand density versus cytoskeletal tension. (ubc.ca)
FERM10
- ERM proteins are made of three domains, the FERM domain, a central helical domain and a C-terminal tail domain, which binds F-actin. (embl.de)
- The amino-acid sequence of the FERM domain is highly conserved among ERM proteins and is responsible for membrane association by direct binding to the cytoplasmic domain or tail of integral membrane proteins. (embl.de)
- ERM proteins are regulated by an intramolecular association of the FERM and C-terminal tail domains that masks their binding sites for other molecules. (embl.de)
- Aside from binding to membranes, the activated FERM domain of ERM proteins can also bind the guanine nucleotide dissociation inhibitor of Rho GTPase (RhoDGI), which suggests that in addition to functioning as a cross-linker, ERM proteins may influence Rho signalling pathways. (embl.de)
- There are 19964 FERM_C domains in 19942 proteins in SMART's nrdb database. (embl.de)
- Taxonomic distribution of proteins containing FERM_C domain. (embl.de)
- The complete taxonomic breakdown of all proteins with FERM_C domain is also avaliable . (embl.de)
- Click on the protein counts, or double click on taxonomic names to display all proteins containing FERM_C domain in the selected taxonomic class. (embl.de)
- In this manuscript, we report the generation of a new FAK dominant negative (FF), composed of the C terminus (FRNK) and the FERM domain of the protein. (biologists.com)
- We show that the FERM domain has a role in the recruitment of FAK on focal adhesions and controls the dynamics of the protein on these complexes. (biologists.com)
Filaments1
- TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. (ppjonline.org)
Regulator1
- 1996). Collectively, these observations indicate Ca2+-reliant proteolysis like a potential regulator of cytoskeletal proteinCprotein relationships during cell motility. (thetechnoant.info)
Bind2
- All members contain the sequence motifs characteristic of all GTP-binding proteins, bind to GDP and GTP with high affinity. (biomedcentral.com)
- Due to the high level of similarity between the Ig repeats, multiple proteins bind at multiple sites of FLNA [ 8 ] . (encyclopedia.pub)
Signal transduction1
- The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
Mediate3
- In both EHEC and EPEC, the proteins that mediate this attachment are encoded in a chromosomal pathogenicity island called the "locus of enterocyte effacement" (LEE) ( 3 ). (cdc.gov)
- In turn, GTP-bound active GTPases can interact with a plethora of different effectors which mediate the different cellular functions of this family of proteins. (biomedcentral.com)
- Filamins are one of the actin-binding proteins that mediate dynamic remodeling during cell movement. (encyclopedia.pub)
Localization1
- Further investigations of this localization, particularly in cytokinesis, permit to conclude that testin is a midbody bulge protein capable to concentrate to this intercellular bridge through its LIM1 and LIM2 domains. (uni.lu)
Associate1
Structures3
- TGB3 is a small integral membrane protein that contains subcellular targeting signals and directs both TGB2 and the helicase domain-containing TGB1 protein to plasmodesmata-associated structures. (microbiologyresearch.org)
- The BMB2 protein associates with endoplasmic reticulum (ER) membranes, accumulates at plasmodesmata-associated membrane bodies and directs the BMB1 helicase to these structures. (microbiologyresearch.org)
- Agrobacterium-mediated transient co-expression in Nicotiana benthamiana leaves revealed that green fluorescent protein (GFP)-fused actin-binding domains of Arabidopsis fimbrin (ABD2-GFP) and mouse talin (TAL-GFP) inhibited the subcellular targeting of TGB3 and BMB2 to plasmodesmata-associated bodies, which resulted in TGB3 and BMB2 accumulation in the cytoplasm in association with aberrant ER structures. (microbiologyresearch.org)
Adaptor protein1
- Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). (gliome.org)
Attachment1
- Integrin-mediated interactions with the extracellular matrix (ECM) are required for the attachment, cytoskeletal organization, mechanosensing, migration, proliferation, differentiation and survival of cells in the context of a multitude of biological processes including fertilization, implantation and embryonic development, immune response, bone resorption and platelet aggregation. (thno.org)
Interactions1
Structural3
- Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
- Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein. (microbiologyresearch.org)
- A structural feature that distinguishes the Rho proteins from other small GTPases is the so-called Rho insert domain located between a β strand and an α helix within the small GTPase domain [ 1 - 3 ]. (biomedcentral.com)
MeSH1
- Cytoskeletal Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
Viral1
- Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes. (microbiologyresearch.org)
Organization1
- It has been suggested that synemin could function as a linker between different cytoskeletal components based on the fact that it interacts with several proteins involved in the organization of the costameres, neuromuscular and myotendinous junctions within striated muscle cells. (biomedcentral.com)
Residues1
- Typically Rho proteins are 190-250 residues long and consist only of the GTPase domain and short terminal C-terminal extensions. (biomedcentral.com)
Interacts1
- FLNA interacts with more than 90 partner proteins to execute multiple cellular functions [ 7 ] , and mainly helps to provide scaffolding to its interacting partners. (encyclopedia.pub)
Chemotaxis1
- Filopodin, a slime mold protein that binds actin and which is involved in the control of cell motility and chemotaxis. (embl.de)
Transmembrane1
- The integrin family of proteins consists of alpha and beta subtypes, which form transmembrane heterodimers. (mechanobio.info)
SuperFamily1
- Rho proteins belong to the Ras superfamily. (biomedcentral.com)
Phosphorylation1
- The level of protein phosphorylation at specific sites varies from less than 1% to greater than 90%, depending on conditions [ 26 ]. (biomedcentral.com)
Proliferation1
- Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. (ppjonline.org)
Kinases1
- The encoded protein is a member of the FAK subfamily of protein tyrosine kinases that included PYK2, but lacks significant sequence similarity to kinases from other subfamilies. (wikipedia.org)
Tyrosine2
- Protein-tyrosine phosphatases PTPN14 and PTP-D1, PTP-RL10 and PTP2E. (embl.de)
- The N-terminal site (1C415 a.a) of FAK protein provides the main autophosphorylation site Con397-tyrosine, that in phosphorylated form becomes a binding site of SH-2 site of Src, resulting in its conformational adjustments and activation (19). (cahrr.org)
19951
- Shuster and Herman, 1995), the function that Ca2+-governed proteolysis has in coordinating cytoskeletal redecorating is certainly undetermined. (thetechnoant.info)
Phosphatase2
- Caenorhabditis elegans protein phosphatase ptp-1. (embl.de)
- protein phosphatase, Mg2+/Mn2+ depen. (gsea-msigdb.org)
Membrane association1
- Domains of tobacco mosaic virus movement protein essential for its membrane association. (microbiologyresearch.org)
MRNA2
- Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. (biomedcentral.com)
- Consistent with a role for talin in adhesome signaling, loss of talin had the same effect on MUC18 and TRPM1 mRNA levels as forcing B16F1 cells into suspension. (ubc.ca)
Roles1
- This occurs through specific modulation of regulatory proteins during the host-pathogen interaction, especially proteins with roles in pathogenesis [ 27 ]. (biomedcentral.com)
Cell6
- Talin, a cytoskeletal protein concentrated in regions of cell-substratum contact and, in lymphocytes, of cell-cell contacts. (embl.de)
- 1997). Actin-associated calpain substrates suggested to have jobs in cell motility are the membrane-bridging proteins talin, the cross-linking protein -actinin and actin binding proteins (ABP-280), as well as the cortical protein spectrin and ankyrin (for review find Croall and DeMartino, 1991). (thetechnoant.info)
- EspB, a protein thought to be involved in pore formation, is transferred to the host cell by this conduit and is found in both the host cell membrane and cytosol ( 7 ). (cdc.gov)
- EspB, with the aid of a second membrane lytic protein, EspD, forms pores in the host cell as part of the translocation mechanism ( 8 ). (cdc.gov)
- Filamin A (FLNA) is a large actin-binding cytoskeletal protein that is important for cell motility by stabilizing actin networks and integrating them with cell membranes. (encyclopedia.pub)
- Actin-binding cytoskeletal proteins are involved in the formation and maintenance of cell shape and morphology in response to external stimuli from surrounding connective tissue [ 1 ] . (encyclopedia.pub)
Family1
- Within their GTPase domains, they share approximately 30% amino acid identity with the Ras proteins and 40-95% identity within the family. (biomedcentral.com)
Mechanical1
- Combined, these experiments provide an integrated vista of the molecular mechanisms-and their associated kinetics-underpinning the mechanical unfolding and refolding of individual proteins when exposed to mechanical load. (bvsalud.org)