• 2015) The 3' addition of CCA to mitochondrial tRNASer(AGY) is specifically impaired in patients with mutations in the tRNA nucleotidyl transferase TRNT1. (rnacentral.org)
  • Perturbing ANGEL2 expression affected the efficiency of pre-tRNA processing, X-box-binding protein 1 ( XBP1 ) mRNA splicing during the unfolded protein response, and tRNA nucleotidyltransferase 1 (TRNT1)-mediated CCA addition onto tRNAs. (rcsb.org)
  • Transfer RNAs (tRNAs) enter ribosome-mediated protein biosynthesis in a translationally competent state, which includes post-transcriptional modifications at various positions, including the anticodon loop, and the presence of an intact single-stranded CCA-sequence at the 3′-terminus that is required for amino acid attachment by the corresponding aminoacyl-tRNA-synthetase [7] . (prolekarniky.cz)
  • tRNA nucleotidyltransferase (N2), proteasome subunit (EC 3.4.25.1) (N3), probable exosome complex RNA-binding proteins 1 (N4), huge ribosomal subunit proteins Retigabine tyrosianse inhibitor L37 Ae (N5) huge ribosomal subunit proteins L15electronic (N7), ribonuclease P (tRNA processing) proteins element 3 (EC 3.1.26.5) (N8), ribonuclease P protein element 2 (EC 3.1.26.5) (N9), prefoldin, chaperonin cofactor (N10), and a predicted exosome subunit containing the IMP4 domain within small nuclear ribonucleoprotein (N11). (cancercurehere.com)
  • Proofreading (editing) of mischarged tRNAs by cytoplasmic aminoacyl-tRNA synthetases (aaRSs), whose impairment causes neurodegeneration and cardiac diseases, is of high significance for protein homeostasis. (cnrs.fr)
  • 42520) hypothetical protein CP001857 tRNA Arcpr_R0002 complement(42639. (go.jp)
  • As a post-doctoral fellow in the laboratory of Eric Phizicky at the University of Rochester School of Medicine, she participated in identification of several previously undiscovered tRNA modification enzymes, including several that exhibit novel enzyme chemistry. (osu.edu)
  • The Jackman laboratory utilizes the principles and techniques of mechanistic enzymology and enzyme kinetics to elucidate the molecular mechanisms of tRNA processing enzymes. (osu.edu)
  • These same principles of mechanistic enzymology, in combination with the powerful approaches of yeast genetics and molecular biology, also allow the lab to investigate the biological function of enzymes in living cells, including a tRNA splicing enzyme and a tRNA methyltransferase whose biological roles in the yeast Saccharomyces cerevisiae are well-defined, but whose possible role(s) in other organisms remain a mystery. (osu.edu)
  • This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase]. (prolekarniky.cz)
  • Some bacteria carry tRNA genes encoding CCA termini, thus the CCA-adding enzyme is primarily involved in repairing damaged CCA ends in these organisms [9] . (prolekarniky.cz)
  • The functional repertoire of the CCA-adding enzyme has been expanded by its recently discovered role in the quality control of hypo-modified tRNAs [12] . (prolekarniky.cz)
  • Thereby, two tRNA-halves (designated 5′- and 3′-tiRNAs) are generated by a ubiquitously expressed enzyme, angiogenin [18] . (prolekarniky.cz)
  • tRNA synthetase class II core domain (G, Seryl-tRNA synthetase N-terminal domain [Interproscan]. (ntu.edu.sg)
  • Here, we show that mammalian cells critically depended on the editing of mitochondrial threonyl-tRNA synthetase (mtThrRS, encoded by ), disruption of which accumulated Ser-tRNA and generated a large abundance of Thr-to-Ser misincorporated peptides in vivo. (cnrs.fr)
  • 4560 valyl-tRNA synthetase CP001857 CDS Arcpr_0004 4868. (go.jp)
  • 55056) tyrosyl-tRNA synthetase CP001857 CDS Arcpr_0065 complement(55112. (go.jp)
  • 2014 The TAIL-seq process starts with removal of abundant noncoding RNAs such as for example rRNA tRNA little nuclear. (researchtoactionforum.org)
  • tRNA synthetases class I (I, Anticodon-binding domain of tRNA, Zinc finger found in FPG and IleRS [Interproscan]. (ntu.edu.sg)
  • Trm10 catalyzes the methylation of guanosine residues at position 9 of several tRNA species, a modification that is conserved throughout eukaryotic tRNAs (Jackman et al. (osu.edu)
  • tRNA m1G9 methyltransferase (Trm10). (osu.edu)
  • 16508 ribonuclease HII CP001857 tRNA Arcpr_R0001 16547. (go.jp)
  • An oxidative-stress activated nuclease, angiogenin, cleaves first within the conserved single-stranded 3′-CCA termini of all tRNAs, thereby blocking their use in translation. (prolekarniky.cz)
  • The second step is transfer of the now activated aminoacyl groups to cognate tRNAs to be ferried to the ribosomes by chaperone proteins. (tutordale.com)
  • Predicted to enable nucleotidyltransferase activity and tRNA binding activity. (nih.gov)
  • In this study, we demonstrate that exposure to oxidative stress results in a quick repression of translation by deactivation of the aminoacyl-ends of all transfer-RNA (tRNA). (prolekarniky.cz)
  • This cleavage, however, does not significantly reduce the level of mature tRNAs, which implies that tiRNAs may rather act as a signal transducer to modulate translation of specific mRNAs, than to globally repress translation [18] . (prolekarniky.cz)
  • During pre-transfer RNA (tRNA) and unconventional messenger RNA (mRNA) splicing, 2',3'-cyclic phosphates are substrates of the tRNA ligase complex, and their removal is critical for recycling of tRNAs upon ribosome stalling. (rcsb.org)
  • CCA addition by tRNA nucleotidyltransferase: polymerization without translocation? (wikipedia.org)
  • The TRNT1 gene encodes tRNA nucleotidyltransferase 1, which catalyzes the addition of cytosine-cytosine-adenosine (CCA) to the ends of cytoplasmic and mitochondrial tRNAs. (bvsalud.org)
  • peptidyl-tRNA hydrolase, ribosome-attached [Ensembl]. (ntu.edu.sg)
  • Many eukaryotes, pre-tRNA molecules are synthesised with a 16 nucleotide 5′-leader, a 14 nucleotide intron and extra 5′- and 3′- nucleotides. (biologydiscussion.com)
  • The exploration group, drove by Teachers Mario Mörl and Sonja Prohaska, zeroed in on proteins called tRNA nucleotidyltransferases, which join three nucleotide building blocks in the succession C-A to little RNAs (move RNAs) in cells. (thetrendingpost.net)
  • Soderberg K, Denekamp L, Nikiforow S, Sautter K, Desrosiers RC, Alexander L. A nucleotide substitution in the tRNA(Lys) primer binding site dramatically increases replication of recombinant simian immunodeficiency virus containing a human immunodeficiency virus type 1 reverse transcriptase. (umassmed.edu)
  • Incubation of either reagent with IleRS resulted in a rapid loss of both the tRNA Ile aminoacylation and isoleucinedependent isotopic ATP-PPi exchange activities. (openbiochemistryjournal.com)
  • The CCA sequence at the 3′ end is important for export of tRNA precursors from the nucleus and essential for tRNA charging with amino acids. (waw.pl)
  • The most prominent examples of RNA genes are transfer RNA (tRNA) and ribosomal RNA (rRNA), both of which are involved in the process of translation. (wikidoc.org)
  • Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. (nih.gov)
  • Lastly, the tRNA undergoes a chain of base modification i.e. modification in 20% of bases to form unusual bases such as ribothymidine (rT), pseudouridine (Ψ), dihydrouridine (DHU) and inosine (I). (biologydiscussion.com)
  • At each end introns are removed by endo-catalytic cleavage followed by ligation of the half molecules of tRNA. (biologydiscussion.com)
  • Maf1 from yeast Saccharomyces cerevisiae mediates repression of trna transcription when cells are transferred from medium with glucose to medium with glycerol, a non-fermentable carbon source. (waw.pl)
  • This effectwas reduced by overexpression of the yeast CCA1 gene encoding ATP(CTP):tRNA nucleotidyltransferase. (waw.pl)
  • In this article we will discuss about the processing of pre-tRNA in E.coli and yeast. (biologydiscussion.com)
  • Balasubramaniam M, Reis RJS, Ayyadevara S, Wang X, Ganne A, Khaidakov M. Involvement of tRNAs in replication of human mitochondrial DNA and modifying effects of telomerase. (uams.edu)
  • Human Thg1 displays tRNA-inducible GTPase activity. (nih.gov)
  • This tRNA shortening is specific for nutritional stress and renders tRNAs unsuitable substrates for translation. (nih.gov)
  • Through a specific step, RNases D, E, F and P generate these mature tRNA molecules by exo-and endo-nucleolytic cleavage (Fig. 10.11 A). (biologydiscussion.com)
  • Once optimal growth conditions are restored tRNAs are rapidly repaired by the trypanosome tRNA nucleotidyltransferase thus rendering the recycled tRNAs amenable for translation. (nih.gov)
  • In this study tRNA precursors were analysed by RNA-Seq and Northern hybridization in wild type strain and maf1Δ mutant grown in glucose medium or upon shift to repressive conditions. (waw.pl)