BD Multitest™ CD3 fluorescein isothiocyanate (FITC)/CD8 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP)/CD4 allophycocyanin (APC) is a four-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3+), suppressor/cytotoxic (CD3+CD8+) T-lymphocyte subsets, and helper/inducer (CD3+CD4+) T-lymphocyte subsets in erythrocyte-lysed whole blood. (bdbiosciences.com)
Using monoclonal antibodies to the surface antigens or suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cell subsets, we characterized peripheral lymphocytes in 27 patients with ulcerative colitis (UC) and 15 age-matched healthy controls by a fluorescence activated cell sorter. (elsevierpure.com)
The lymphocytophilic antibodies were also demonstrated to be reactive with non-helper, non-suppressor T cell subsets and some populations of helper T cells. (elsevierpure.com)
The activated IL-2 receptor, expressing cells belonged to both CD4 subsets, 'helper inducer' (CD44B4) and 'suppressor inducer', (CD42H4). (ox.ac.uk)
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells. (nih.gov)
Receptors2
Increased numbers of T lymphocytes bearing the interleukin-2 (IL-2) receptors were found in intraocular fluids or peripheral blood or both of 35/51 patients with uveitis. (bmj.com)
This increased expression of IL-2 receptors on lymphocytes correlated with increased expression of other early T lymphocyte activation markers, HLA-DR and L-35. (bmj.com)
Cells26
HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
The virus infects mature T lymphocytes in vitro, although other cells can serve as targets. (aao.org)
Immunological studies among jaundiced patients revealed significant changes in T-helper and T-suppressor cells among chronic HBV cases from the acute and control groups. (who.int)
In the BALF obtained after NO2 exposure and in the baseline state, the total number of lymphocytes and the percentages of T cells (CD 3), B cells (CD 20), T cytotoxic-suppressor cells (CD 8), T helper-inducer cells (CD 4), and large granular lymphocytes (CD 57) also did not differ after NO2 exposure. (researchgate.net)
A slightly but significantly greater proportion of natural killer cells (CD 16) was found in the BALF obtained after NO2 exposure (7.2 +/- 3.1 vs 4.2 +/- 2.4% of total lymphocytes). (researchgate.net)
Both T helper cells (Leu-3A+) and suppressor cells (Leu 2A+) were activated in vivo. (bmj.com)
These data show that some patients with idiopathic uveitis have a perturbation of T helper cells. (bmj.com)
For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions. (bvsalud.org)
The important role of both helper and suppressor T cells in this regulation is quite apparent. (johnshopkins.edu)
HIF-1α- and hypoxia-dependent immune responses in human CD4+CD25high T cells and T helper 17 cells. (uni-luebeck.de)
Lymphoid tissue inducer cells express a diverse array of tumor necrosis family ligands, including those that bind CD30 and the lymphotoxin 13 receptor. (birmingham.ac.uk)
Reduced expression of CCL21 due to CD30 deficiency was functionally significant: mice deficient in both lymphotoxin and CD30 (dKO) signals had significantly smaller accumulations of lymphocytes in their splenic white pulp areas, with no evidence of focal aggregation of T cells. (birmingham.ac.uk)
We suggest that CD30 signals from lymphoid tissue inducer cells were a primitive mechanism to recruit and prime CD4 T cells. (birmingham.ac.uk)
BACKGROUND AND OBJECTIVES: Follicular helper T (Tfh) cells play a critical role in protective immunity helping B cells produce antibodies against foreign pathogens and are likely implicated in the pathogenesis of various autoimmune diseases. (regionh.dk)
T follicular helper (Tfh) cells and T peripheral helper (Tph) cells produce interleukin (IL)-21 and are thought to contribute to follicular and extra-follicular B-cell activation, respectively, in autoimmune diseases. (elsevierpure.com)
Intracellular cytokine staining and T-cell-B-cell co-culture studies indicated that IFN-α promotes generation of IL-21+IFN-γ+CXCR5-CD4+ T cells thereby enhancing B-cell helper function. (elsevierpure.com)
In recent onset insulin-dependent diabetes blood there was no lack of CD4 CD45R+ (suppressor/inducer) T cells. (ox.ac.uk)
In contrast to the ease of cloning and characterizing, at the molecular level, helper and cytotoxic T cells, suppressor T cells remain an enigma, and their existence as discrete entities is being increasingly challenged. (ox.ac.uk)
Since some therapeutic approaches in various human diseases are based on the manipulation of helper and suppressor functions, the question whether committed suppressor cells exist has important practical implications in medicine. (ox.ac.uk)
Some of these will then activate the adaptive immunity cells, the lymphocytes, by showing them antigens from the infectious agents. (reverdy.fr)
This activation starts either by direct contact with the pathogenic agent (for the B lymphocytes), or via the appearance of a pathogenic antigen by cells presenting antigens such as phagocytic cells taking part in the innate immune reaction. (reverdy.fr)
Cytotoxic T lymphocytes , responsible for cell immunity, that act by releasing granules containing cytotoxins which attack the membranes and DNA of infected cells, leading to their death. (reverdy.fr)
Additionally, the frequency of helper and cytotoxic T cells was reduced during the 1(st) wave, whereas CD69 expression in helper T cells was increased in the 2(nd) wave for both H1N1 and ILI patients. (ox.ac.uk)
Virus-specific T-helper cells are considered critical for the control of chronic viral infections. (ox.ac.uk)
Antigens3
Lymphocyte blastogenesis assay in response to different mitogens and antigens produced two groups: responders (acute cases) and nonresponders (chronic cases). (who.int)
Circulating and BALF lymphocytes were labeled with fluorochrome-conjugated monoclonal antibodies to human lymphocyte antigens and a flow cytometer was used to count lymphocyte subtypes. (researchgate.net)
Because the helper T lymphocytes are central to the immune response, loss of this subset results in a profound immune deficiency, leading to the life-threatening opportunistic infections indicative of AIDS. (aao.org)
This application note demonstrates the use of the NovoCyte flow cytometer for the analysis of human blood lymphocyte subset. (selectscience.net)
These patients are unable to mount an antibody response to novel T lymphocyte-dependent B-lymphocyte challenges, although they have B-lymphocyte hyperfunction with polyclonal B-lymphocyte activation, hypergammaglobulinemia, and circulating immune complexes. (aao.org)
This B-lymphocyte hyperfunction may be a direct consequence of HIV infection: studies have demonstrated that polyclonal activation can be induced in vitro by adding HIV to B lymphocytes. (aao.org)
In idiopathic uveitis activation of Leu-3A lymphocytes (helper/inducer) was significantly increased, and intraocular activation of the Leu-2A lymphocytes (cytotoxic/suppressor) was significantly decreased. (bmj.com)
A prior activation phase before any action of lymphocytes is essential. (reverdy.fr)
Regulatory4
Here we review evidence that CD4+ regulatory clones, capable of expressing both helper and suppressor functions, may account for much of the suppressor function. (ox.ac.uk)
T-Lymphocytes, Regulatory" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
This graph shows the total number of publications written about "T-Lymphocytes, Regulatory" by people in this website by year, and whether "T-Lymphocytes, Regulatory" was a major or minor topic of these publications. (wakehealth.edu)
Below are the most recent publications written about "T-Lymphocytes, Regulatory" by people in Profiles. (wakehealth.edu)
Subpopulations1
CD3+ T lymphocytes can be further divided into CD4+ T helper/inducer and CD8+ suppressor/cytotoxic subpopulations. (selectscience.net)
Follicular helper1
BAFF also contributed to the maintenance and/or expansion of the follicular helper T cell population, although it was dispensable for their formation. (duke.edu)
It also shows dermal infiltration by T lymphocytes of both helper-inducer and suppressor-cytotoxic phenotypes, a pattern commonly seen with lichen planus. (medscape.com)
Splenic1
Animal studies have suggested that NO2 exposure causes a decrease in the numbers of some splenic lymphocyte subtypes and impairs lymphocyte-dependent immune responses. (researchgate.net)
Antibodies1
B lymphocytes , responsible for humoral immunity, by producing specific antibodies against the infectious agent. (reverdy.fr)
Interleukin1
The prevailing dogma that IBD was mediated by interleukin (IL)-12-driven T-helper (Th)1 CD4 T cell responses towards the bacterial flora has been largely dispelled by findings that the closely related cytokine IL-23 appears to be the key mediator of intestinal inflammation. (ox.ac.uk)
Depletion2
The hallmark of the immunodeficiency in AIDS is a depletion of the CD4 + helper-inducer T lymphocytes. (aao.org)
This selective depletion of CD4 + helper T lymphocytes leads to the characteristic inverted CD4 + /CD8 + ratio (also known as the T4/T8 ratio ), where the ratio drops to less than 1.0, in comparison to the normal CD4 + /CD8 + ratio, which is around 2. (aao.org)
Auxiliaires1
Les études immunologiques effectuées chez des malades atteints d'un ictère ont révélé des changements importants au niveau des lymphocytes T suppresseurs et des cellules T auxiliaires chez les cas chroniques d'hépatite B par comparaison avec les groupes de cas aigus et de témoins. (who.int)
Proliferation2
Cross-linking of T3 (CD3) with T4 (CD4) enhances the proliferation of resting T lymphocytes. (bdbiosciences.com)
However, this effect appeared to occur in the presence of high concentrations of propolis, while at low concentrations the effect is reversed, causing stimulation of lymphocyte proliferation. (ijpsonline.com)
Responses1
We observed increased virus-specific cytotoxic T lymphocytes and maintained T-helper-cell responses in all. (ox.ac.uk)
Immunodeficiency1
In addition to cellular immunodeficiency, patients with AIDS have abnormalities of B-lymphocyte function. (aao.org)
Ratio1
A reversed T-suppressor/helper cell ratio was noted as the only immunologic abnormality. (duke.edu)
Human1
To investigate whether ambient levels of NO2 alter circulating and bronchoalveolar lavage fluid (BALF) human lymphocytes, we studied five healthy nonsmoking adult volunteers. (researchgate.net)
Patients3
Le test de prolifération lymphocytaire en réponse aux différents mitogènes et antigènes a produit deux groupes: les patients répondants (cas aigus) et les patients non-répondants (cas chroniques). (who.int)
Les deux groupes étaient semblables en ce qui concerne leur réponse proliférative aux antigènes de surface de l'hépatite B (HBsAg) mais une réponse vigoureuse aux antigènes centraux de l'hépatite B (HBcAg) était une caractéristique importante chez les patients répondants. (who.int)
To test the role of T helper cell Th1 immunity we recruited 24 patients with idiopathic thrombocytopenia associated with H. pylori seropositivity. (who.int)
Signals2
It is suggested that a single T cell clone, depending on the signals it receives from its environment, may release either helper or suppressor cytokines. (ox.ac.uk)
Studying such clones under defined conditions (providing suppressor signals), may preclude detection of their helper capacity. (ox.ac.uk)
Enumeration of CD4+ lymphocytes in HIV-positive participants and age-matched controls was performed on cryopreserved whole blood using the method reported by Fiebig et. (cdc.gov)