• Here we show that an affinity proteomics strategy using affinity-purified antibodies raised against recombinant human protein fragments can be used for chromosome-wide protein profiling. (nih.gov)
  • A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)
  • In this paper, the optimized gene AMP BSN-37 was cloned into pCold-SUMO for fusion expression by recombinant DNA technology. (bvsalud.org)
  • The recombinant plasmid was constructed and identified by PCR, enzyme digestion and sequencing. (bvsalud.org)
  • The recombinant plasmid pCold-SUMO-BSN-37 was obtained, and the fusion AMP BSN-37 was preliminarily expressed in BL21. (bvsalud.org)
  • The gene encoding a (2 R ,3 R )-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (ReBDH) was over-expressed in Escherichia coli and the resulting recombinant ReBDH was successfully purified by Ni-affinity chromatography. (mdpi.com)
  • To circumvent the need for BSL-3 laboratories, an alternative assay was developed that avoids live virus and instead uses a recombinant VSV expressing luciferase and possesses the full length or truncated spike proteins of SARS-CoV-2. (biomedcentral.com)
  • An integrated framework for cDNA library production, sequencing, quality control, expression data generation, and systems-level analysis is presented and utilized. (biomedcentral.com)
  • a loblolly pine (pinus taeda l.) cdna with properties of a nonspecific lipid transfer protein (nsltp) is reported. (liverpool.ac.uk)
  • two genes preferentially expressed in differentiating xylem of loblolly pine (pinus taeda l.) were cloned from cdna and genomic libraries and designated ptx3h6 and ptx14a9. (liverpool.ac.uk)
  • Functional annotation of gene sequences indicate functional classifications are not randomly distributed among gene families containing few or many genes. (biomedcentral.com)
  • Primary annotations based on sequence similarity are linked to networks of systematic annotation in Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and can be queried and computationally utilized in downstream analyses. (biomedcentral.com)
  • This is due to a paucity of functional annotation from sequence homology, which is compounded by low genetic tractability compared with many model organisms. (portlandpress.com)
  • Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. (nih.gov)
  • They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. (genomicglossaries.com)
  • Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
  • No cannabinoid receptors are expressed in sponges, which probably diverged before the origin of the eumetazoan ancestor. (researchgate.net)
  • The valuable source of large-scale genomic information initiated attempts to identify the origin(s) of G protein-coupled receptors (GPCR), count and categorize those genes, and follow their evolutionary history. (springer.com)
  • Schioth HB, Fredriksson R (2005) The GRAFS classification system of G-protein coupled receptors in comparative perspective. (springer.com)
  • Sequence comparison does not support an evolutionary link between halobacterial retinal proteins including bacteriorhodopsin and eukaryotic G-protein-coupled receptors. (springer.com)
  • Soybean has a large (1115 Mbp) genome that has yet to be fully sequenced. (biomedcentral.com)
  • Such EST data form a valuable foundation for the understanding of the gene composition and genomic biology of yet-to-be fully sequenced genomes [ 3 ]. (biomedcentral.com)
  • Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
  • An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
  • A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 ( MP43 ) of X. laevis. (xenbase.org)
  • The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
  • The advent of genome sequencing revealed in humans and other species thousands of genes encoding proteins that had not been identified by previous biochemical or genetic studies. (biorxiv.org)
  • Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
  • Bardet-Biedl syndrome 5 protein is a protein that in humans is encoded by the BBS5 gene. (wikipedia.org)
  • Vaccination by direct injection of lentiviral vector was more effective in sterile alpha motif and HD-domain containing protein 1-knockout (SAMHD1-knockout) mice, suggesting that lentiviral vectors containing Vpx, a lentiviral protein that increases the efficiency of dendritic cell transduction by inducing the degradation of SAMHD1, would be an effective strategy for the treatment of chronic disease in humans. (jci.org)
  • We present evidence for the de novo origin of the Wookiee wookiee protein-coding gene GLABR since their divergence from humans. (ubc.ca)
  • Recent studies suggest that most of the genome are transcribed, among the transcripts only a small portion encode for proteins, whereas a large portion of the transcripts do not encode any proteins, which are generally termed non-protein coding RNAs (npcRNA). (biomedcentral.com)
  • Strains were transformed with nudE variants in pAid vector and grown at 43°C. Numbers refer to amino acid residues of NUDE protein expressed by the constructs (see Fig. 3 for detailed amino acid sequences). (xenbase.org)
  • Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. (frontiersin.org)
  • After Blast analysis within GenBank, 64% could be identified, 21% were homologous to unannotated expressed sequence tag (EST) or genomic sequences, and 15% were novel. (appliedbioinfo.com)
  • Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
  • These and many other functional genomics studies rely on full genomic sequence to establish well-annotated databases. (biomedcentral.com)
  • About a quarter are required for viability, and functional screening of the rest revealed hits for fertility, development, locomotion, protein quality control and resilience to stress. (biorxiv.org)
  • However, identification of long npcRNAs is limited to a few plant species, such as Arabidopsis, rice and maize, no systematic identification of long npcRNAs and their responses to abiotic and biotic stresses is reported in wheat. (biomedcentral.com)
  • In addition, post-translational modifications can also contribute to the increased complexity and diversity of protein species [ 3 ]. (biomedcentral.com)
  • These processes have left footprints in the genomic sequence that can be detected by bioinformatic methods and may help to interpret receptor function in the light of a given species in its environment. (springer.com)
  • Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)
  • Phylogenetic comparisons to Brachypodium, rice, and Arabidopsis NAC proteins indicate that the barley NAC family includes members from all of the eight NAC subfamilies, although by comparison to these species a number of HvNACs still remains to be identified. (biomedcentral.com)
  • Fujisawa Y, Kato H, Iwasaki Y (2001) Structure and function of heterotrimeric G proteins in plants. (springer.com)
  • The S protein is the main target with which the neutralizing antibodies react. (biomedcentral.com)
  • The neutralization antibody test (NT) for serum using live SARS-CoV-2 is a method in which inhibition of the serum upon viral infection is observed in the presence of neutralizing antibodies against proteins involved in viral binding and penetration in the serum. (biomedcentral.com)
  • The genes of human chromosome 21 identified by the genome efforts were investigated, and the success rates for de novo cloning, protein production, and antibody generation were 85, 76, and 56%, respectively. (nih.gov)
  • An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
  • The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues. (nih.gov)
  • Genes preferentially expressed in bovine oocytes revealed by subtractive and suppressive hybridization. (appliedbioinfo.com)
  • B-cell translocation gene 4, cullin 1, MCF.2 transforming sequence, a locus similar to snail soma ferritin, and three unidentified genes were, indeed, preferentially expressed in the oocyte, even though most were also highly expressed in testis. (appliedbioinfo.com)
  • The approach is based on affinity reagents raised toward bioinformatics-designed protein epitope signature tags corresponding to unique regions of individual gene loci. (nih.gov)
  • Genomic sequencing of selected loci indicate that intron-like intervening sequences are numerous and are approximately 220 bp in length. (biomedcentral.com)
  • The more recent duplication event in particular would be expected to result in many paralogous pairs of genes differing by relatively few sequence differences, thus complicating gene identification using ESTs. (biomedcentral.com)
  • We describe a bioinformatics pipeline known as FunnyBase that has been used to store, annotate, and analyze 40,363 expressed sequence tags (ESTs) from the heart and liver of the fish, Fundulus heteroclitus . (biomedcentral.com)
  • Many EST libraries were constructed from RNA extracted from various genetic backgrounds, thus gene identification from these sources is complicated by the existence of both gene and allele sequence differences. (biomedcentral.com)
  • CRISPRi genetic interaction analysis revealed Retro-2 activity resembles disruption of the transmembrane domain recognition complex (TRC) pathway, which mediates post-translational ER-targeting and insertion of tail-anchored (TA) proteins, including SNAREs required for retrograde transport. (elifesciences.org)
  • The pore-forming BCL-2 family proteins mediate mitochondrial poration to initiate apoptosis through protei-protein and protein-lipid interactions. (portlandpress.com)
  • The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
  • Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
  • In 2013, the genome sequences of O. ulmi and O. novo-ulmi were publicly released. (sisef.it)
  • 2005) The genome sequence of the rice blast fungus Magnaporthe grisea. (prelekara.sk)
  • Since the release of the first draft of the human genome sequence in 2000, the function of many of these new proteins has been identified. (biorxiv.org)
  • Large scale gene analysis of most organisms is hampered by incomplete genomic sequences. (biomedcentral.com)
  • Analysis of these sequences revealed single base substitutions and single base indels are the most frequently observed form of sequence variation between genes within families in the dataset. (biomedcentral.com)
  • Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae -specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome ( Fig. 1 ). (nature.com)
  • Systematic Analysis of ZnCys Transcription Factors. (prelekara.sk)
  • Systematic Analysis of ZnCys Transcription Factors Required for Development and Pathogenicity by High-Throughput Gene Knockout in the Rice Blast Fungus. (prelekara.sk)
  • Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
  • The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
  • Efimov VP and Morris NR (2000), The LIS1-related NUDF protein of Aspergillus ni. (xenbase.org)
  • The LIS1 -related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein. (xenbase.org)
  • NUDF protein interacts with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. (xenbase.org)
  • Burton, R.S., Metz, E.C., Flowers, J.M., Willett, C.S. (2005) Unusual structure of ribosomal DNA in the copepod Tigriopus californicus: intergenic spacer sequences lack internal sub repeats. (mapress.com)
  • Furthermore, the adaptation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated protein 9 (CRISPR/Cas9) technology has dramatically improved gene editing efficiency at the single gene level. (portlandpress.com)
  • In the present study, we isolated RNA aptamers specific to influenza NS1 protein. (datexis.com)
  • Western blotting showed that the fusion peptide was successfully expressed and had good activity. (bvsalud.org)
  • Endogenous synthesis of antigen results in efficient proteolytic peptide processing and presentation of peptide antigen on MHC class I proteins. (jci.org)
  • Alternatively, the DCs are transduced with a viral vector that expresses the antigen. (jci.org)
  • The probes have been used for molecular hybridizations (fluorescence in situ hybridization, or FISH) in clinical cytogenetics, Y chromosome mapping, sequencing, gene discovery, and comparative Y chromosome studies between the horse and other equids/ Perssodactyls. (veteriankey.com)
  • Therefore, microdissected Y-chromosome probes are good for FISH but not suitable for cloning and sequencing of the male-specific or pseudoautosomal regions of the chromosome. (veteriankey.com)
  • Together, our work explains how Retro-2 prevents retrograde trafficking of toxins by inhibiting TA-protein targeting, describes a general CRISPR strategy for predicting the MOA of small molecules, and paves the way for drugging the TRC pathway to treat broad classes of viruses known to be inhibited by Retro-2. (elifesciences.org)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)
  • The S protein is also important for induction of protective humoral and cellular immunity during infection. (biomedcentral.com)
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
  • 2008), which is responsible for sequence variations of different virus strains. (datexis.com)
  • The 576-aa DEAF-1 protein possesses two conserved domains, SAND and MYND. (sdbonline.org)
  • This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
  • Cleavage was performed at where can you get clarinex each Bownes stage from pole cell migration due to the number of plaques formed on lawns of bacteria expressing the MM15 crRNA (15). (billfryer.com)
  • We compared infection of bacteria expressing crRNAs with where can you get clarinex and without mismatches to crRNAs with. (billfryer.com)
  • Antiphage small molecules produced by bacteria - beyond protein-mediated defenses. (spp2330.de)
  • Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
  • Kim JY, Haastert PV, Devreotes PN (1996) Social senses: G-protein-coupled receptor signaling pathways in Dictyostelium discoideum . (springer.com)
  • Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
  • If, on the other hand, A. nidulans and A. fumigatus are assumed to have lost 7-9 Mb of sequence after branching off from their A. oryzae -like ancestor, a greater proportion of syntenic blocks would be conserved between each of them and A. oryzae than between the two. (nature.com)
  • A) Homology between A. nidulans NUDE protein and N. crassa RO11 protein. (xenbase.org)
  • The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
  • Moreover, SENP5 inhibits the SUMOylation level of E2F transcription factor 1 (E2F1) and increases the protein expression of E2F1. (bvsalud.org)
  • Several polymorphisms in TS may influence TS mRNA transcription, stability, or protein expression. (biomedcentral.com)
  • Several features of lentiviral vectors have made them advantageous for the expression of therapeutic proteins in vivo. (jci.org)
  • This gene has no protein-coding homolog in any other genome but its presence is supported by evidence from expression and hybridization data. (ubc.ca)
  • Thirteen Arabidopsis and six rice NAC transcription factors have been shown to contain an α-helical transmembrane motif in the far C-terminal region which anchors the NAC protein to intracellular membranes rendering them inert. (biomedcentral.com)
  • Nordstrom KJ, Sallman Almen M, Edstam M et al (2011) Independent HHsearch, Needleman-Wunsch-based, and motif analyses reveal the overall hierarchy for most of the G protein-coupled receptor families. (springer.com)
  • Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is required for the formation of cilia. (wikipedia.org)
  • In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of antigen-expressing lentiviral vector, obviating the need for ex vivo transduction of dendritic cells. (jci.org)
  • S protein is a glycoprotein, which is responsible for binding and penetration of target cells. (biomedcentral.com)
  • Through searches in publicly available barley sequence databases we have obtained a list of 48 barley NAC genes ( HvNACs ) with 43 of them representing full-length coding sequences. (biomedcentral.com)
  • NUDF protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. (xenbase.org)
  • The identification of potential gene sequences (pHaps) from soybean allows us to begin to get a picture of the genomic history of the organism as well as begin to observe the evolutionary fates of gene copies in this highly duplicated genome. (biomedcentral.com)
  • Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), DEAF-1 was identified as a candidate regulator. (sdbonline.org)
  • Structural biology has been used to elucidate their mechanisms of activation, dimerization, and interaction with membranes, and AlphaFold, which relies solely on sequence information, can accurately predict the activated states, from Moldoveanu and colleagues, pages 35 to 47. (portlandpress.com)
  • The expressed BSN-37 showed antimicrobial activity similar to that of synthesized BSN-37. (bvsalud.org)
  • The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)
  • Typical NAC transcription factor, with N-terminal NAC domain, consisting of five conserved subdomains, A-E. The C-terminal part of the protein is more variable and contains the transcriptional activation or repressing region. (biomedcentral.com)
  • 2007). This allowed isolation of ECAY sequences and generation of Y-specific molecular probes. (veteriankey.com)
  • 1992). Due to the largely heterochromatic nature of ECAY and repeat-biased amplification of DOP-PCR, microdissected probes tend to be enriched with repetitive sequences, while euchromatic sequences remain underrepresented. (veteriankey.com)
  • Several well-known oocyte-specific genes were represented, including growth differentiation factor 9, bone morphogenetic protein 15, or the zona pellucida glycoprotein genes. (appliedbioinfo.com)
  • Of 768 clone inserts submitted for differential screening by macroarray hybridization, 83% displayed a fourfold overexpression in the oocyte. (appliedbioinfo.com)
  • are highly expressed in the human brain and show a strikingly similar regional distribution. (biorxiv.org)
  • If the bait and prey proteins indeed interact then the two factors fused to these two proteins are also brought into proximity with each other. (genomicglossaries.com)
  • The fact that NUDF and LIS1 interact with the same protein domain strengthens the notion that these two proteins are functionally related. (xenbase.org)
  • In many organisms, such as soybean, the best source of sequence information is the existence of expressed sequence tag (EST) libraries. (biomedcentral.com)
  • We developed a microarray-based approach for the systematic discovery of tissue-specific genes and, by applying it to Foxo3 ovaries and other samples, defined a surprisingly large set of ovarian factors (n = 348, approximately 1% of the mouse genome). (appliedbioinfo.com)
  • The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
  • Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)