System development zebrafish. Medical search. Frequent questions

To try to overcome some of these limitations in this study, we have used Caenorhabditis elegans (nematode) and Danio rerio embryos (zebrafish) as alternative assays for DART hazard assessment of some candidate chemicals being considered for a new commercial application. (nih.gov)
The expression pattern of this gene family has been recently described during zebrafish ( Danio rerio ) embryogenesis, and primarily locates in the nervous system. (frontiersin.org)
To this end, the zebrafish ( Danio rerio ) is an established model to study early nervous system development, which combines the complexity of a vertebrate organism with the easy-to-use and high-throughput capabilities of in vitro models ( MacRae and Peterson, 2015 ). (frontiersin.org)
The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family (Cyprinidae) of the order Cypriniformes. (wikipedia.org)
Zebrafish (Danio rerio) is a small fish of the Cyprinidae family originating in the streams of India and Myanmar. (weizmann.ac.il)
E. J. Flynn, C. M. Trent and J. F. Rawls, "Ontogeny and Nutritional Control of Adipogenesis in Zebrafish (Danio rerio)," Journal of LIPID Research, Vol. 50, No. 8, 2009, pp. 1641-1652. (scirp.org)
Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio). (oregonstate.edu)
Zebrafish (Danio rerio) embryos were exposed to 0 ppb, 0.3 ppb, 3 ppb, or 30 ppb ATZ during embryogenesis (1-72 h post fertilization (hpf)), then rinsed and raised to maturity. (cdc.gov)
Chronic toxicity: There are data for 3 trophic levels, most sensitive fish (zebrafish Danio rerio) NOEC 0.0003 microg/L. (janusinfo.se)

The zebrafish also exhibits measurable behavioral and hormonal responses already at the larval stages, providing a viable vertebrate animal model for high-throughput drug screening and chemical genetics. (weizmann.ac.il)
Through an array of larval zebrafish behavior assays, we found that zebrafish lacking all NMDARs had robust behavioral alterations, including abnormal responses to light and acoustic stimuli, and diminished prey capture abilities. (zantiks.com)
We elucidated the role of histamine in wakefulness and the interaction of the histamine and hcrt systems in larval zebrafish. (elsevierpure.com)
Our data suggests AHR2 regulates some aspect(s) of neuromuscular and/or sensory system development, with impaired behavioral responses observed in larval and adult AHR2-null zebrafish. (oregonstate.edu)
2022. Efficacy of tricaine (MS-222) and hypothermia as anesthetic agents for blocking sensorimotor responses in larval zebrafish. (awionline.org)
Novel cross-regulation interactions between dlx genes in larval zebrafish. (nih.gov)
Here we show that transient disruption of brain morphogenesis by ethanol exposure results in behavioral hyperactivity in larval zebrafish challenged with changes in lighting conditions. (oregonstate.edu)

Here, we describe in detail the spatiotemporal expression of three rprm genes ( rprma, rprmb , and rprm l) within distinct anatomical structures in the developing peripheral and central nervous system. (frontiersin.org)
With the available tools of the genomic era and the abundance of disease-associated human genes yet to be explored, the zebrafish model is becoming the preferred choice in basic and applied life sciences. (weizmann.ac.il)
Because of this, we have begun to generate zebrafish containing human missense mutations in the endogenous zebrafish genes using prime editing. (zantiks.com)
Gene expression analysis indicates that esrra is expressed in developing pharyngeal arches where genes necessary for cartilage development are also expressed. (nature.com)
Expression of esrra is colocalised with genes necessary for cartilage development in pharyngeal arches during zebrafish embryogenesis. (nature.com)
The zebrafish shares 70 % of the genes found in humans and many critical signalling pathways. (europa.eu)
We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. (researchsquare.com)
Conclusions: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. (researchsquare.com)
In the present project, we showed that zebrafish rab11 genes are conserved in vertebrate evolution by in silicon analysis. (researchsquare.com)
Lxr ligand activation affected the expression of genes involved in lipid metabolism in zebrafish adult brain and eye as well as in zebrafish embryos. (scirp.org)
We study genes, proteins and signaling pathways that might be essential for RGC and optic nerve development, function, survival, and regeneration. (nih.gov)
This requires parallel studies on genes that are important for the function of the retina, the optic nerve and aqueous humor outflow system in the normal eye. (nih.gov)
2021. Nitrate-induced improvements in exercise performance are coincident with exuberant changes in metabolic genes and the metabolome in zebrafish skeletal muscle. . (oregonstate.edu)
These approaches allow the Maves lab to use the zebrafish to understand how specific genes work to properly form the heart. (seattlechildrens.org)
Small molecules identified in this way offer potential to dissect further these and other developmental processes and to identify novel genes involved in vertebrate development. (broadinstitute.org)
The generation of new models will be based on candidate genes identified in WP1 and/or WP2The consortium also aims to use knowledge from genetic and biomarker discovery programmes (in particular, novel pathway information) to support development of novel animal models, and includes European players with a strong track record in this area. (lu.se)
The techniques will include generation of models featuring tissue-specific disruption of selected genes using Cre-loxP systems (in adipocytes, muscle, endothelium, pericytes and myocardium as required). (lu.se)
PAX6 provides instructions for making proteins that drive tissue development in the fruit fly eye by turning on 'eye' genes and turning off 'non-eye' genes. (medlineplus.gov)

Herein we examine the temporal and spatial expression patterns of RPRM gene-products (mRNA and protein) during neural development. (frontiersin.org)
Surprisingly, we found that zebrafish lacking all NMDAR function survive longer than their murine counterparts, offering a unique opportunity to interrogate NMDAR roles in neural development. (zantiks.com)
however, its role in neural development in vivo remains inconclusive. (curie.fr)
The goal of the ZENITH ETN, "ZEbrafish Neuroscience Interdisciplinary Training Hub", is to understand how neural networks mediate perception and behavior. (europa.eu)
Recently, we showed that Wnt8a is transported on short, actin-based filopodia known as cytonemes to contact responding cells and activate signalling during neural plate formation in zebrafish (1).Wnt/ Ror2 signalling regulates the formation of these Wnt cytonemes (5). (exeter.ac.uk)
A study in mouse cell lines demonstrated Grb2 was necessary in facilitating cortical neuron growth, which displays the importance of Grb2 in development of neural tissues (4). (uvm.edu)
One of the first things that appears as an embryo forms is a brain primordium and the neural tube, which will form the nervous system and "innervate" - supply with nerves - all organs and body structures. (scienceblog.com)
Without vitamin E, the zebrafish embryos showed neural tube defects and brain defects. (scienceblog.com)
In healthy organisms, neural crest cells drive the creation of facial bones and cartilage and innervate the body, building the peripheral nervous system. (scienceblog.com)
We have identified several small molecules that specifically modulate various aspects of vertebrate ontogeny, including development of the central nervous system, the cardiovascular system, the neural crest, and the ear. (broadinstitute.org)
The results demonstrate that developmental exposure to ATZ does affect neurodevelopment and neural function in adult male zebrafish and raises concern for possible health effects in humans due to ATZ's environmental presence and persistence. (cdc.gov)
These cancers originate from cells of the sympathetic nervous system (SNS), in turn derived from the transient embryonic neural crest stem cell population. (lu.se)
Paragangliomas are on the other hand slow growing tumors and mostly benign, but also these are believed to be primed during development of the neural crest. (lu.se)
Our main model organism is the chick embryo , ideal for functional and translational studies on neural crest development. (lu.se)
To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)

Here, we establish the relevancy of the zebrafish model to study developmental functions of NMDARs in vivo. (zantiks.com)
In this report, we establish an in vivo role of Esrra in cartilage development during embryogenesis in zebrafish. (nature.com)
However, more direct evidence using an animal model is necessary to demonstrate the in vivo role of ESRRa in chondrocyte development. (nature.com)
Rapid and sensitive biomarkers and bioassays, both in vitro (e.g. protein binding assays, enzyme induction assays) and in vivo (e.g. zebrafish embryo assay) methods to measure the exposure, bioavailability, and effects of chemicals with endocrine disrupting potency are available. (vu.nl)
A strong advantage of the zebrafish model is the availability of in vivo imaging techniques, because the embryo is completely transparent and can live for several days without a functional cardiovascular system. (uni-potsdam.de)
This study aims to establish the role of redox regulation on steroidogenesis using zebrafish as a vertebrate in vivo model.In contrast to humans, zebrafish have two F. (endocrine-abstracts.org)
Zebrafish has emerged as an important vertebrate in vivo model to study human disease. (endocrine-abstracts.org)

In the area of toxicology a number of cell culture systems are available but while these have some predictive value, they are not ideally suited for the prediction of developmental and reproductive toxicology (DART). (nih.gov)
In zebrafish Piperazine and analogs showed developmental delays. (nih.gov)
The results of the nematode and zebrafish studies were in alignment with data obtained from conventional mammalian toxicity studies indicating that these have potential as developmental toxicity screening systems. (nih.gov)
This method can help answer key questions in the developmental biology field because it allows direct observation of tissues and organ development in living embryos over several hours. (jove.com)
Though this method can provide insights into spacial and temporal alterations that occur during development of sweeper fish embryo and larvae, it can also be applied to investigate early developmental processes and other model organisms. (jove.com)
Zebrafish lacking any of the GluN2(A-D) subunits survive into adulthood, affording an opportunity to assay subunitspecific developmental effects. (zantiks.com)
Together, these findings highlight the unique opportunity to study, in zebrafish, the roles of NMDARs in development and disease etiology and afford a system for future examination of the developmental effects of disease-associated missense mutations. (zantiks.com)
A complex expression pattern of ESRRs during animal development seems to be consistent with the potential roles for ESRRa during appropriate developmental programs of tissues and organs in mouse and zebrafish 9 . (nature.com)
The eggs are fertilised externally, facilitating developmental interventions and studies, and the resulting embryos are transparent, meaning development can be watched under a microscope. (europa.eu)
Function of FGF signaling in the developmental process of the median fin fold in zebrafish. (nih.gov)
While the role of miRNAs in establishing and maintaining the developing nervous system is widely appreciated, the developmental neurobehavioral role of miRNAs has yet to be defined. (oregonstate.edu)
Although Rab11a subcellular localization has been demonstrated by transgenic zebrafish [10], and molecular function of Rab11 proteins in primary cilia membrane assembly in Kupffer Vesicle has been studied [6], the developmental expression profile of Rab11 proteins in zebrafish remains elusive. (researchsquare.com)
Vitamin E (VitE) is necessary for embryo development and its deficiency causes developmental errors that occur at embryologically similar periods in rodents, humans and zebrafish. (oregonstate.edu)
However, the precise developmental profile of functional zebrafish BBB acquisition and the subcellular and molecular mechanisms governing the zebrafish BBB remain poorly characterized. (elifesciences.org)
Our findings indicate a conserved developmental program of barrier acquisition between zebrafish and mice. (elifesciences.org)
Small molecule developmental screens reveal the logic and timing of vertebrate development. (broadinstitute.org)
Here, we demonstrate that small molecules capable of conditional gene product modulation can be identified through developmental screens in zebrafish. (broadinstitute.org)
Molecular and morphological changes in zebrafish following transient ethanol exposure during defined developmental stages. (nccu.edu)
Adult male zebrafish behavior, brain transcriptome, brain methylation status, and neuropathology were examined to test the hypothesis that embryonic ATZ exposure causes delayed neurotoxicity, according to the developmental origins of health and disease paradigm. (cdc.gov)

Zebrafish Models to Study NMDA Receptors in Early Nervous System Development and Disease [Doctoral thesis, State University of New York at Stony Brook]. (zantiks.com)
It is well established that cholinergic signaling has critical roles during central nervous system development. (oregonstate.edu)
Vitamin E is necessary for zebrafish nervous system development. (oregonstate.edu)
His longstanding research interest has been the role of the extracellular matrix in nervous system development, especially the function of the heparan sulfate proteoglycan agrin. (nccu.edu)
These studies are currently focusing on elucidating the mechanisms by which agrin modulates the function of heparin-binding growth factors and morphogens, such as fibroblast growth factors (Fgfs) and sonic hedgehog (Shh), during zebrafish nervous system development. (nccu.edu)
Chick embryos are easily accessible, and their nervous system development is similar to that of human embryos at comparable stages. (lu.se)

By in situ hybridization we showed distinct expression of lxr in the brain and the retina in the developing and adult zebrafish. (scirp.org)
Our data show that lxr is an important component of the regulatory network governing the lipid homeostasis during zebrafish development, which in turn may support a role of lxr for normal development of the central nervous sytem, including the retina. (scirp.org)
Retina development in zebrafish requires the heparan sulfate proteoglycan agrin. (nccu.edu)
Tiny zebrafish can also regrow parts of the eye, including neurons in the eye's retina (the light-sensitive tissue at the back of the eyeball). (medlineplus.gov)

In this report, we used the zebrafish model to examine the role of esrra in cartilage development during vertebrate embryogenesis. (nature.com)
Vitamin E deficiency dysregulates thiols, amino acids and related molecules during zebrafish embryogenesis. (oregonstate.edu)
Zebrafish Cyp11a1 (zCyp11a1) is essential during embryogenesis. (endocrine-abstracts.org)

These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (nih.gov)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

2017). The not-so-long history of zebrafish research in Israel. (weizmann.ac.il)
2017). Comparison of molecular marker expression in early zebrafish brain development following chronic ethanol or morpholino treatment. (nccu.edu)

Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. (nih.gov)
2020). Ethanol and cannabinoids regulate zebrafish GABAergic neuron development and behavior in a sonic hedgehog and fibroblast growth factor dependent mechanism. (nccu.edu)

AHR2 required for normal behavioral responses and proper development of the skeletal and reproductive systems in zebrafish. (oregonstate.edu)
The zebrafish is a powerful model system that provides a vertebrate data stream that anchors hypothesis at the genetic and cellular levels to observations at the morphological and behavioral level, in a high-throughput format. (oregonstate.edu)
A goal of Dr. Cole's laboratory is to correlate these molecular changes, as a consequence of embryonic ethanol exposure, to behavioral changes in juvenile and adult zebrafish. (nccu.edu)
Long-term behavioral impairment following acute embryonic ethanol exposure in zebrafish. (nccu.edu)
Embryonic atrazine exposure and later in life behavioral and brain transcriptomic, epigenetic, and pathological alterations in adult male zebrafish. (cdc.gov)

We will exploit the advantages of zebrafish as a small transparent vertebrate with superb genetic accessibility and use cutting-edge technology to elucidate the interactions between molecules, cells and entire networks that ultimately generate adaptive behavior. (europa.eu)
Understanding how the brain transforms sensory input into complex behavior is a fundamental question in systems neuroscience. (harvard.edu)
2019). Ethanol and cannabinoids interact to alter behavior in a zebrafish fetal alcohol spectrum disorder model. (nccu.edu)

A simple ball of cells is the starting point for humans - and zebrafish. (ist.ac.at)
Scientists are always in search of model systems that can help them study in the simplest, fastest and most effective ways disease pathways that affect humans. (europa.eu)
By using the zebrafish as a model of CMT2A, (which a ffects the distal axons of motor and sensory neurons in humans), the study was able to examine how the mitofusin 2 (MFN2) affects the phenotype in the zebrafish. (hnf-cure.org)
Zebrafish embryos are studied because their neurodevelopment has a high degree of genetic homology to humans. (oregonstate.edu)
Because the zebrafish genome and early heart development are very similar to that in humans, work in the Maves lab will have direct relevance for understanding human heart development and congenital heart defects. (seattlechildrens.org)
Zebrafish share a remarkable similarity to humans at the molecular, genetic and cellular levels, meaning many findings are immediately relevant to humans. (scienceblog.com)
Since zebrafish share 70% of their genetic information with humans and have similar pathways for the CNR1 and CNR2 receptors, they were chosen as model organisms to carry out this study (Burke) (Lee Ellis). (belmont.edu)

Activation of α2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish. (oregonstate.edu)
Embryonic zebrafish are of special interest because they develop quickly, are transparent and are easy to care for. (scienceblog.com)
Agrin function associated with ocular development is a target of ethanol exposure in embryonic zebrafish. (nccu.edu)

At 36 weeks, histological evaluations of the AHR2-null ovaries revealed a reduction of mature follicles when compared to wild type ovaries, suggesting AHR2 regulates follicle growth in zebrafish. (oregonstate.edu)
Estrogen-related receptor alpha (ESRRa) regulates a number of cellular processes including development of bone and muscles. (nature.com)
As a doctoral student in Brian Link's lab at MCW, my studies centered on the Hippo pathway which regulates organ size development. (mcw.edu)

Zebrafish are amenable to genetic manipulation, for which innovative genetic and molecular techniques are constantly being introduced. (weizmann.ac.il)
Many researchers have turned to the humble zebrafish to further their understanding of genetic disorders, and this investigation was no exception. (hnf-cure.org)
They are taking advantage of CRISPR-Cas9 genome editing technology, and of zebrafish as an animal model system, to identify the genetic causes of congenital heart defects. (seattlechildrens.org)
These studies are employing genetic and molecular approaches to examine ocular, forebrain, and hindbrain development in response to ethanol exposure, and the subsequent changes in agrin, Fgf or Shh signaling. (nccu.edu)
The development of caged MOs (cMOs) has significantly extended the versatility of these reverse-genetic tools. (cdc.gov)

scientists with a deep insight into genetics, biomarker discovery, development of novel imaging assays for assessing progression of complications, novel animal models for chronic diabetic complications, in silico models to predict development of complications and response to novel treatments. (lu.se)

MZrgs4 embryos showed motility defects and presented reduced head and eye sizes, reflecting defective motoneurons axon outgrowth and a significant decrease in the number of neurons in the central and peripheral nervous system. (curie.fr)

In order to investigate the endogenous functions of AHR, we generated an AHR2 (homolog of human AHR)-null zebrafish line ( ahr2 osu1) using the clustered, regulatory interspaced, short palindromic repeats (CRISPR)-Cas9 precision genome editing method. (oregonstate.edu)
With their expertise in using CRISPR-Cas9 genome editing technology in zebrafish, the Maves lab have the capability to engineer knock-out and precise knock-in zebrafish strains. (seattlechildrens.org)
The aquatics facility at Seattle Children's Research Institute is well-equipped with tools for using CRISPR-Cas9 in zebrafish. (seattlechildrens.org)

N-methyl-D-aspartate receptors (NMDARs) are glutamate-gated ion channels that play critical roles in nervous system function and neuronal development. (zantiks.com)
L. Wang, G. U. Schuster, K. Hultenby, Q. Zhang, S. Andersson and J. A. Gustafsson, "Liver X Receptors in the Central Nervous System: From Lipid Homeostasis to Neuronal Degeneration," Proceedings of the National Acadamy of Sciences of the USA, 15 October 2002, pp. 13878-13883. (scirp.org)
Cannabinoids are lipid, retrograde neurotransmitters have been shown to act mostly on CNR1 and CNR2 (cnr1 and cnr2 in zebrafish respectively) receptors which modulate many pathways in the central nervous system. (belmont.edu)
Previous work from our lab showed that treatment of zebrafish embryos with a synthetic cannabinoid, WINN55,212-2, led to premature hatching, morphological changes, and a downregulation of each of the cannabinoid receptors. (belmont.edu)
We investigated the role of these receptors further by treating zebrafish embryos with a half dose of WINN55,212-2 at various times during its development. (belmont.edu)

Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family. (frontiersin.org)
Such knowledge is fundamental to advance in our understanding of the possible role of this gene family in neuronal development. (frontiersin.org)
Moreover, zebrafish is very well-suited for systematic analysis of gene expression patterns at transcript level by whole-mount in situ hybridization (WISH), and at protein level by immunohistochemistry/immunofluorescence (IHC/IF). (frontiersin.org)
The team will emerge with skills in visualisation, gene editing and computational modelling to study the nervous system and enhance understanding from cells to circuits to behaviour. (europa.eu)
Optogenetic Dissection of Neuronal Circuits in Zebrafish using Viral Gene Transfer and the Tet System. (nih.gov)
We explore gene factors and how they affect the development of the heart and blood vessels. (ncl.ac.uk)
We research gene expression control during development and misregulated in diseases. (ncl.ac.uk)
We then analyzed the temporal and spatial expression of rab11a gene in embryonic development by RT-PCR and whole mount in situ hybridization. (researchsquare.com)
Thus, VitE plays a critical role in maintaining the developing nervous system by ensuring proper gene expression and localization during embryo development to subsequently prevent physical deformation of the brain and peripheral nervous system. (oregonstate.edu)
Much has been learned about vertebrate development by random mutagenesis followed by phenotypic screening and by targeted gene disruption followed by phenotypic analysis in model organisms. (broadinstitute.org)
Introduction: Steroid 5-α reductase type 2 deficiency causes 46,XY disorder of sex development (DSD) and is an autosomal recessive disorder resulting from mutations in the SRD5A2 gene. (endocrine-abstracts.org)
NIH R21 AA025400, P.I., "Gene-ethanol interactions in a zebrafish multi-binge FASD model," $405,079 total costs. (nccu.edu)
Transcriptomic analysis identified altered gene expression in organismal development, cancer, and nervous and reproductive system development and function pathways and networks. (cdc.gov)

Adult zebrafish are fed VitE sufficient (E+) or deficient (E-) diets and then are spawned to produce E+ and E- embryos. (oregonstate.edu)

Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. (researchsquare.com)
Among various Rab proteins, Rab11 is known to localize to recycling endosome and regulate vesicular recycling and primary ciliogenesis, playing a key role in Kupffer vesicle development in zebrafish [5-7]. (researchsquare.com)
The zebrafish Rab11a, Rab11ba and Rab11bb proteins are highly conserved during evolution, especially the GTPase domain, suggesting their important functions. (researchsquare.com)
Zebrafish rab11a was clustered in the Rab11a group, and rab11ba and rab11bb in the Rab11b group (Fig.1B) in the phylogenic tree constructed according to amino acid sequences of all the Rab11 proteins above. (researchsquare.com)
To study function of these proteins we also use zebrafish as a model system. (nih.gov)
The highly conserved family of Wnt proteins represents important regulators of cell behaviour, tissue development and homeostasis by inducing responses in a concentration-dependent manner. (exeter.ac.uk)
Adaptor proteins are critical for the development of many tissues and function by tethering upstream signals to downstream molecules to initiate and/or propagate cellular responses (1). (uvm.edu)

Research areas Through programs spanning genetics, biology, and therapeutic development, Broad researchers are making discoveries that drive biomedical science forward. (broadinstitute.org)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

In this study, we expressed thymosin ß (Tß) in zebrafish in vitro and studied its antimicrobial function. (bvsalud.org)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

Defects in ADA2 caused intracranial bleeding in the animals, hinting at a role in blood vessel development or integrity. (nih.gov)
In particular, they are engineering zebrafish to carry DNA mutations that are found in human patients with heart defects. (seattlechildrens.org)
Farr GH 3rd, Imani K, Pouv D, Maves L. Functional testing of a human PBX3 variant in zebrafish reveals a potential modifier role in congenital heart defects . (seattlechildrens.org)

MicroRNAs (miRNAs) and other classes of short non-coding RNAs regulate essential processes in the development and function of the nervous system. (harvard.edu)

The transparency of the zebrafish embryo allows real-time visualization of the development and morphogenesis of practically all of its tissues and organs. (weizmann.ac.il)

They are highly prized for studying the development and genetics of vertebrates. (scienceblog.com)

I am interested in the molecular mechanisms during development and morphogenesis. (uni-potsdam.de)
At the Living Systems Institute, we collaborate with biophysicists using super-resolution microscopy to describe these signalling processes in a quantitative way on a molecular level. (exeter.ac.uk)
However, the subcellular and molecular mechanisms governing the formation and maintenance of the zebrafish BBB are only beginning to be characterized. (elifesciences.org)

We show that friction is generated by forming tissues sliding against each other, and that this force is a key mechanism for regulating morphogenesis during embryo development," Carl-Philipp Heisenberg explains. (ist.ac.at)
The first main topic is modeling, analysis, and discovery of Bone Morphogenetic Protein signaling networks in Drosophila stem cells maintenance and embryo development. (purdue.edu)
Our study provides new insight into the rab11 expression and promotes the use of this model organism to tackle future studies on the role of rab11 in embryo development. (researchsquare.com)
vitamin E is the straw whose extraction brings down the house on embryo development, especially with the brain and nervous system. (scienceblog.com)

The major emphasis of the laboratory is the investigation of the role of agrin in fetal alcohol spectrum disorders (FASD) and how agrin function via Fgfs and Shh is a target of embryonal ethanol and cannabinoid exposure in zebrafish. (nccu.edu)

In situ hybridization demonstrates ubiquitous expression of grb2a and grb2b in the zebrafish nervous system. (uvm.edu)

Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

Importantly, a precise temporal description of RPRM protein expression during the development of neuronal structures is still missing. (frontiersin.org)
Kao RM, Rurik JG, Farr GH 3rd, Dong XR, Majesky MW, Maves L. Pbx4 is required for the temporal onset of zebrafish myocardial differentiation . (seattlechildrens.org)

In 2015, a study was published about zebrafishes' capacity for episodic memory. (wikipedia.org)
In the present study, Heisenberg and his group studied the mechanical forces that are at work when the central nervous system (CNS) of the zebrafish develops. (ist.ac.at)
This study aimed to determine the prevalence of keel bone deviations and feather damage of laying hens in two different free-range housing systems under commercial conditions. (awionline.org)
The objective of this study was to determine the role of LXR during development using the zebrafish model. (scirp.org)
We use a zebrafish model to study functions of Olfm1 in development. (nih.gov)
In this study, we use zebrafish as a model to investigate Grb2's role in the nervous system. (uvm.edu)
As an optically transparent model organism with an endothelial blood-brain barrier (BBB), zebrafish offer a powerful tool to study the vertebrate BBB. (elifesciences.org)
The goal for this study is to have a better understanding of how the use of cannabinoids can affect embryonic development when they are introduced at varying stages of embryonic development in zebrafish. (belmont.edu)
NIH-supported researchers continue to study fruit fly development, asking questions like, "What makes an eye an eye? (medlineplus.gov)

Advanced optical methods and genome-editing will be utilized to link molecules to circuits, to map connectivity between cell types across the nervous system and to build computational models of brain activity that will be experimentally tested. (europa.eu)

The histaminergic and hypocretin/orexin (hcrt) neurotransmitter systems play crucial roles in alertness/wakefulness in rodents. (elsevierpure.com)
However, aside from muscle development, the roles of ESRRs in other tissues including bone and cartilage have just begun to be investigated 10 . (nature.com)

Thymosin hormones, which were shown to be involved in immune system development and differentiation in previous studies, have antimicrobial functions in different animals. (bvsalud.org)

Zebrafish widely used as an animal model in various fields of biomedical research as a disease model for cancer, metabolic and neurodegenerative diseases, and regenerative medicine. (weizmann.ac.il)

The zebrafish is an important and widely used vertebrate model organism in scientific research, for example in drug development, in particular pre-clinical development. (wikipedia.org)
Expanding our understanding of the zebrafish BBB can thus reveal the mechanistic similarities between the zebrafish and mammalian BBB to further evaluate the position of zebrafish as a model organism for studying the BBB. (elifesciences.org)

Forebrain and hindbrain development in zebrafish is sensitive to ethanol exposure involving agrin, Fgf and Sonic hedgehog function. (nccu.edu)

Additionally, loss of Grb2 leads to abnormalities in the peripheral nervous system, and retinal patterning. (uvm.edu)
The current research emphasis of Dr. Cole's laboratory is to understand the mechanisms of ethanol- and cannabinoid-induced central nervous system abnormalities in zebrafish. (nccu.edu)

Structural analyses performed on adults showed that repression of miR-153c during development impacts craniofacial skeletal development. (oregonstate.edu)
Together, these data support an integral role for miRNAs in the establishment of vertebrate neurobehavioral and skeletal systems. (oregonstate.edu)

If trade-offs exist between somatic growth and reproduction for this species, a reduction in the physiological condition of swamp fish should be accompanied by reduced development of reproductive tissue (gonad mass), as found in other fish (Wu et al. (researchgate.net)

To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. (nih.gov)
Our research includes the use of knockout mice and zebrafish as laboratory models. (ncl.ac.uk)

The focus of our research is to investigate the regulation of signal transduction in development. (purdue.edu)
Zebrafish are frequently used as a means to investigate development. (awionline.org)
In our research, we use the zebrafish embryo to investigate how intercellular Wnt protein transport is regulated and how signals are subsequently delivered to the target cell in a living vertebrate organism. (exeter.ac.uk)
To investigate the interrelationship between these two systems further, we have studied the orexinergic inputs to the TM nucleus using intracellular recordings and single-cell PCRs from TM neurons as well as immunocytochemical methods. (jneurosci.org)

Tissue development is a key process for life starting from the earliest embryonic stages during which cells differentiate into later organs composing an entire body. (exeter.ac.uk)

Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. (awionline.org)
In research with key ramifications for women of childbearing age, findings by Oregon State University scientists show that embryos produced by vitamin E-deficient zebrafish have malformed brains and nervous systems. (scienceblog.com)
Zebrafish is a well-established model in translational vascular research. (endocrine-abstracts.org)
Zebrafish are a useful model for immunology research. (bvsalud.org)

By understanding Wnt dissemination mechanisms, we will be able to control Wnt signalling in development, and regeneration. (exeter.ac.uk)
However, the mechanisms by which viruses cross the blood-brain barrier (BBB) and invade into the central nervous system (CNS) remain unclear. (bvsalud.org)

However, direct evidence regarding its involvement in cartilage development remains elusive. (nature.com)

The EU-funded ZENITH project is preparing a new generation of scientists for interdisciplinary collaboration on pressing neuroscience challenges utilising the zebrafish model system and leveraging academic and industrial mentoring. (europa.eu)

Consistent with a role for miRNAs in neurobehavioral development, target prediction analyses identified multiple miRNAs misexpressed in the ethanol-exposed cohorts that were also predicted to target inversely expressed transcripts known to influence brain morphogenesis. (oregonstate.edu)

2016). Zebrafish reel in phenotypic suppressors of autism. (weizmann.ac.il)

The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. (cshlpress.com)

In summary, our findings provide evidence that these two systems are linked both functionally and developmentally, which may have important implications in sleep disorders and narcolepsy. (elsevierpure.com)
These findings also indicate that functional neuronal nAChRs are present within spinal cord at the time when locomotor output in zebrafish first begins to manifest itself. (oregonstate.edu)
These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (nih.gov)
The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos. (researchsquare.com)

An imaging system for zebrafish behaviour and development is available. (vu.nl)
Due to the conserved nature of vertebrate cell behaviour, our results will be relevant to Wnt signalling during human embryonic development and could suggest novel vulnerabilities to Wnt-dependent diseases - a prerequisite for the development of novel therapeutics. (exeter.ac.uk)

We also analyzed the role of Akt, Erk and mechanistic target of rapamycin (mTOR) signaling cascades and showed a requirement for these pathways in motoneurons axon outgrowth and neuronal development. (curie.fr)
Mechanosensitive Notch-Dll4 and Klf2-Wnt9 signaling pathways intersect in guiding valvulogenesis in zebrafish. (uni-potsdam.de)

The aryl hydrocarbon receptor (AHR) is a conserved ligand-activated transcription factor required for proper vertebrate development and homeostasis. (oregonstate.edu)
Taken together, our results indicate for the first time that ESRRa is essential for cartilage development by regulating sox9 expression during vertebrate development. (nature.com)

These results suggest that histamine is essential for the development of a functional and intact hcrt system and that histamine has a bidirectional effect on the development of the hcrt neurons. (elsevierpure.com)

There are, in conjunction with the genomics platforms built with SWEGENE support, in general systems for the immediate management of the large amounts of experimental data generated. (lu.se)

2018) In: Development and function of the zebrafish neuroendocrine system. (weizmann.ac.il)
Here, we generated and characterized a rgs4 loss of function model (MZrgs4) in zebrafish. (curie.fr)
CECR1 codes for ADA2, a poorly characterized protein thought to be a growth factor that plays a role in immune system function. (nih.gov)
To gain insights into the protein's function, the scientists turned to zebrafish. (nih.gov)
MicroRNAs control neurobehavioral development and function in zebrafish. (oregonstate.edu)
Although thymosin has been reported to be involved in the embryonic development of zebrafish, it is necessary to uncover the antimicrobial function of thymosin in zebrafish. (bvsalud.org)
For example, we have applied light-activatable cMOs to interrogate transcription factor function during zebrafish notochord, pancreas, and vascular patterning. (cdc.gov)

Anatomy71

Organisms20

Diseases8

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology3

Phenomena and Processes65

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science8

Health Care3

Danio9

Larval7

Genes18

Neural15

Vivo7

Developmental19

Nervous system development6

Retina4

Embryogenesis3

Transgenic2

20172

Neuron2

Behavioral5

Behavior3

Humans7

Embryonic Zebrafish3

Regulates3

Genetic5

Assays1

Embryos showed1

CRISPR-Cas93

Receptors5

Gene13

Adult1

Proteins7

Biology2

Vitro2

Defects3

Regulate1

Tissues and organs1

Vertebrates1

Molecular3

Embryo development4

Cannabinoid exposure1

Situ hybridization1

Endogenous1

Temporal2

Study9

Genome1

Roles2

Differentiation1

Regenerative1

Model organism2

Ethanol exposure1

Abnormalities2

Skeletal2

Reproductive1

Knockout2

Investigate4

Organs1

Research4

Mechanisms2

Remains elusive1

Neuroscience1

Morphogenesis1

20161

Drosophila1

Findings4

Behaviour2

Pathways2

Vertebrate development2

Functional1

Genomics1

Function7