Protein EngineeringTissue EngineeringGenetic EngineeringDNA ShufflingMetabolic EngineeringModels, MolecularMolecular Sequence DataDirected Molecular EvolutionAmino Acid SequenceProtein FoldingSubtilisinBiomedical EngineeringEscherichia coliMutagenesis, Site-DirectedProtein ConformationKineticsInteinsMutationProtein StabilityProteinsEnzyme StabilityBiotechnologyProtein Structure, SecondaryMutagenesisRecombinant ProteinsBinding SitesStructure-Activity RelationshipSynthetic BiologyTissue ScaffoldsProtein Structure, TertiaryProtein SplicingCrystallography, X-RayBase SequenceSubstrate SpecificityPeptide LibraryBacterial ProteinsAmino Acid SubstitutionCatalysisMolecular StructureBiophysical ProcessesMutant ProteinsBiocompatible MaterialsStereoisomerismBacteriophage M13ThermodynamicsRecombinant Fusion ProteinsProtein BindingPhosphoribosylglycinamide FormyltransferaseCell Surface Display TechniquesTyrosine-tRNA LigaseProtein BiosynthesisGenes, SyntheticProtein DenaturationBioengineeringDNAEnzymesHydroxymethyl and Formyl Transferasesalpha-AmylasesCircular DichroismDrug DesignPeptidesTemperatureBacillusCell EngineeringPlasmidsCloning, MolecularMagnetic Resonance SpectroscopyChemical EngineeringSequence AlignmentModels, ChemicalBiosensing TechniquesCell LineSequence Homology, Amino AcidCells, CulturedAmino AcidsElectrophoresis, Polyacrylamide GelCysteineTriose-Phosphate IsomeraseGene LibraryDNA PrimersTitrimetryCrystallizationHydrogen-Ion ConcentrationCatalytic DomainProtein Synthesis InhibitorsGeobacillus stearothermophilusRNA, MessengerNuclear Magnetic Resonance, BiomolecularComputer SimulationDisulfidesOxidation-ReductionSaccharomyces cerevisiaeMuramidaseLigandsBioartificial OrgansSubtilisinsMixed Function OxygenasesGenetic VectorsPlant ProteinsHydrophobic and Hydrophilic Interactions