Synthase escherichia coli proteins. Medical search. Frequent questions

Biosynthesis of aromatic amino acids in plants, many bacteria, and microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, a prime target for drugs and herbicides. (rcsb.org)
Glyphosate appears to occupy the binding site of the second substrate of EPSP synthase (phosphoenol pyruvate), mimicking an intermediate state of the ternary enzyme.substrates complex. (rcsb.org)
Bifunctional (p)ppGpp synthase/hydrolase SpoT or SpoT is a regulatory enzyme in the RelA/SpoT Homologue (RSH) protein family that synthesizes and hydrolyzes (p)ppGpp to regulate the bacterial stringent response to environmental stressors. (wikipedia.org)
protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
The recombinant protein had enzymatic properties similar to those of the plant enzyme. (weedscience.com)
The enzyme was dimeric and was slightly inhibited by ATP (Ki= 1.9 mM for acetyl coenzyme A), which is a known inhibitor for many eukaryotic, dimeric citrate synthases. (nih.gov)
NADH, an allosteric inhibitor of prokaryotic hexameric citrate synthases, did not affect enzyme activity. (nih.gov)
Unlike most prokaryotic dimeric citrate synthases, the enzyme did not have any methylcitrate synthase activity. (nih.gov)
A unique feature of the enzyme, in contrast to citrate synthases from both eukaryotes and prokaryotes, was a lack of stimulation by K+ ions. (nih.gov)
Refinement of the model, obtained using phases derived from the structure of the same enzyme from Escherichia coli by molecular replacement, is in progress. (cnr.it)
Active site cysteine, histidine, and lysine residues in the beta 2 subunit of E. coli tryptophan synthase are conserved in the yeast enzyme. (unipr.it)
Also conserved in the yeast enzyme are 6/8 amino acid residues having an important role in maintaining the structure and function of the E. coli tryptophan synthase alpha subunit. (unipr.it)

Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
A pathway for stilbene biosynthesis was constructed in Escherichia coli with 4-coumaroyl CoA ligase 1 4CL1) from Arabidopsis thaliana and stilbene synthase (STS) cloned from Arachis hypogaea . (biomedcentral.com)
Engineered route to stilbene biosynthesis in E. coli . (biomedcentral.com)
We aimed to develop a high-throughput functional metagenomic screening system for identifying novel terpene synthases from bacterial metagenomes by relieving the toxicity of terpene biosynthesis precursors to the Escherichia coli host. (biomedcentral.com)
Escherichia coli and Saccharomyces cerevisiae are the two host systems that have been most actively engineered as platforms for the biosynthesis of terpenes and their derivatives [ 5 ]. (biomedcentral.com)
Both strains have native metabolic pathways generating prenyl pyrophosphate precursors for the terpene biosynthesis, namely, the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway in E. coli and the mevalonate (MVA) pathway in S. cerevisiae [ 6 ]. (biomedcentral.com)

SpoT and its homologues have been studied in bacterial model organism E.coli for their role in the production and degradation of (p)ppGpp in the stringent response pathway. (wikipedia.org)
Despite its importance in central metabolism and bacterial cell signaling, protein histidine phosphorylation has remained elusive with respect to its extent and functional roles in biological systems because of the lack of adequate research tools. (princeton.edu)
To achieve this goal we use a combination of x-ray crystallography, molecular modelling, molecular docking and molecular biology in collaboration with medicinal chemistry to engineer drugs that specifically interact with and disable critical bacterial target proteins. (ubc.ca)
Recent genomics investigations have predicted a large untapped reservoir of bacterial terpene synthases residing in the genomes of uncultivated organisms living in the soil, indicating a vast array of putative terpenoids waiting to be discovered. (biomedcentral.com)

Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12. (microbiologyresearch.org)
Expression of a valine-resistant acetolactate synthase activity mediated by the ilvO and ilvG genes of Escherichia coli K-12. (microbiologyresearch.org)
The relative levels of specific terpenes in the glandular trichomes of each cultivar were correlated with the levels of transcripts for eight genes encoding distinct terpene synthases. (myspecies.info)
Our results showed that there were thirty-one FvSUN genes in F. vesca and the FvSUNs encoded proteins were classified into seven groups, and the members in the same group showed high similarity in gene structures and conservative motifs. (bvsalud.org)
Phenylpropionic acids, such as 4-coumaric acid and caffeic acid, can be efficiently converted to stilbene compounds by recombinant E. coli cells expressing plant biosynthetic genes. (biomedcentral.com)

Penicillin-binding protein 1B (PBP1B) of Escherichia coli is a bifunctional murein synthase containing both a transpeptidase domain and a transglycosylase domain. (ncl.ac.uk)
Yeast tryptophan synthase, a bifunctional protein, has a primary structure which corresponds to an Escherichia coli tryptophan synthase alpha chain-beta chain fusion. (unipr.it)

Beta-ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue. (genebiosystems.com)
In addition, the transient time for the coupled reaction sequence was less for the fusion protein than for the free enzymes. (lu.se)
Prior sequencing of the genome of Geobacter sulfurreducens revealed a putative citrate synthase sequence related to the citrate synthases of eukaryotes. (nih.gov)
The deduced coding sequence for tryptophan synthase contains 2,127 base pairs. (unipr.it)
A portion of the connecting region of yeast tryptophan synthase exhibits nucleotide sequence similarity to the 3' terminus of E. coli trpC and the trpC-trpB intercistronic region. (unipr.it)
Our functional metagenomic screening of human fecal metagenomes yielded a novel β-farnesene synthase, which does not show amino acid sequence similarity to known β-farnesene synthases. (biomedcentral.com)

The acetohydroxy acid synthase III isoenzyme of Escherichia coli K-12: regulation of synthesis by leucine. (microbiologyresearch.org)
Optimization of precursor conversion and cyclization of the bulky ferulic acid precursor by host metabolic engineering and protein engineering may afford the synthesis of even more structurally diverse stilbene compounds. (biomedcentral.com)
A six-lobed membrane spanning cellulose synthesis complex (CSC) containing multiple cellulose synthase (CESA) glycosyltransferases mediates cellulose microfibril formation. (osti.gov)
Acute exposure to COV resulted in a reduction in mean arterial and diastolic blood pressures 1 and 28 d after exposure, changes in nitrate-nitrite and H(2)O(2) levels, and in the expression of transcripts and proteins that regulate inflammation, vascular remodeling, and the synthesis of NO in the heart and kidneys. (cdc.gov)

Mutant of Escherichia coli K-12 missing acetolactate synthase activity. (microbiologyresearch.org)
A single amino acid substitution from Tryptophan 574 to Leucine has led to resistance to Inhibition of Acetolactate Synthase as indicated in the table below. (weedscience.com)
Acetolactate synthase (ALS) inhibitors are among the most commonly used herbicides. (weedscience.com)

The total amounts of terpenes were correlated with total levels of terpene synthase activities, and negatively correlated with levels of phenylpropanoids and phenylalanine ammonia lyase activity. (myspecies.info)
this precursor toxicity can be alleviated by channeling the excessive prenyl pyrophosphates into terpenes through an exogenous terpene synthase [ 9 ]. (biomedcentral.com)

Citrate synthase activity of these organisms is of interest due to its key role in acetate metabolism. (nih.gov)
All citrate synthase activity in G. sulfurreducens could be resolved to a single 49-kDa protein via affinity chromatography. (nih.gov)
Similar citrate synthase sequences were detected in a diversity of other Geobacteraceae members. (nih.gov)
This first characterization of a eukaryotic-like citrate synthase from a prokaryote provides new insight into acetate metabolism in Geobacteraceae members and suggests a molecular target for tracking the presence and activity of these organisms in the environment. (nih.gov)

Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. (biomedcentral.com)
An NH2-terminal 239 amino acid segment of yeast tryptophan synthase is homologous with E. coli tryptophan synthase alpha subunit, while a distal 389 amino acid residue segment is homologous to the E. coli tryptophan synthase beta chain. (unipr.it)

Protein domains observed in members of the RSH protein family are separated by hydrolase (HD) functionality and synthetase (SYNTH) functionality (see Figure 2). (wikipedia.org)

Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
Therefore, molecular docking was also used in the present study to simulate the binding of cordycepin with different antibacterial targets, such as β-ketoacyl-(acyl-carrier-protein) synthase III (FabH), DNA gyrase B and thymidylate kinase, in order to identify the most likely antimicrobial mechanism of cordycepin. (spandidos-publications.com)
Structural comparisons with TruB demonstrate that all Psi synthases are descended from a common molecular ancestor. (genesilico.pl)
The protein chain has a calculated molecular weight of 76,544. (unipr.it)

UniProt: the universal protein knowledgebase in 2021. (chesterlecturesociety.org)

β-Ketoacyl-acyl carrier protein synthase II of Escherichia coli. (illinois.edu)
Enoyl-(Acyl carrier protein) reductase [Interproscan]. (ntu.edu.sg)

In a cultivar that synthesizes mostly geraniol, transcripts of geraniol synthase were the most abundant, but the glands of this cultivar also contained a transcript of an (R)-LIS gene with a 1-base insertion that caused a frameshift mutation. (myspecies.info)
Furthermore, structural analysis of Mm HCP C67Y and characterization of several Mm HCP variants with a Cys67 mutation reveal the significance of Cys67 in protein structure, metallocluster binding and hydroxylamine reductase activity. (nature.com)

protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)

When the gene for encoding RelA is nonfunctional, E. coli can still regulate (p)ppGpp through SpoT as it has both HD and SYNTH domains. (wikipedia.org)
A geraniol synthase-LIS hybrid gene was constructed and expressed in Escherichia coli, and the protein catalyzed the formation of both geraniol and (R)-linalool from geranyl diphosphate. (myspecies.info)
The promoter of the AOX1 gene has become a popular tool to drive the expression of recombinant proteins. (biomedcentral.com)

We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)

Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins for a variety of species such as anti-Human N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Xenopus laevis N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Cow N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase). (antibodies-online.com)

This is the expected M(r) for the fusion protein subunit. (lu.se)

E.coli uses RelA and SpoT as its two main (p)ppGpp regulating enzymes. (wikipedia.org)
The kinetics of CS1 and MDH1 activities of the fusion protein were compared to those of the free enzymes. (lu.se)
It was observed that AAT was a less effective competitor for OAA when the CS1/MDH1 fusion protein is used than when the separate enzymes are employed. (lu.se)
Fe-S clusters are present in more than 300 different types of enzymes or proteins and play crucial roles in fundamental life processes such as respiration, photosynthesis and nitrogen fixation. (uga.edu)
protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)

Growth inhibition of Escherichia coli K-12 by valine: a consequence of a regulatory pattern. (microbiologyresearch.org)
Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B. (ubc.ca)

2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
In vivo, the acetohydroxy acid synthase (AHAS) isoenzymes have an affinity for α-KB sufficiently high that α-acetolactate production is severely limited when α-KB is supplied exogenously. (microbiologyresearch.org)

E7 from human papil omavirus type 16 cooperate to cancer: the European prospective investigation into doi:10.1093/carcin/bgp321 PMID:20047954 target the PDZ protein Na/H exchange regulatory cancer and nutrition study. (who.int)

2015 . The crystal structure of full-length E. coli YidC revealed that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans , which lacks a periplasmic domain. (tcdb.org)
Structural characterization of SpoIIIAB sporulation-essential protein in Bacillus subtilis. (ubc.ca)
Structural and kinetic analyses of penicillin-binding protein 4 (PBP4)-mediated antibiotic resistance in Staphylococcus aureus. (ubc.ca)

Isoleucine and valine metabolism in Escherichia coli. (microbiologyresearch.org)
Homocystinuria is an inherited autosomal recessive defect in methionine metabolism that is caused by a deficiency in cystathionine synthase. (medscape.com)

They then export some of these proteins as well some mitochondrially-encoded proteins to the inter membrane space, or they insert them into the inner membrane. (tcdb.org)
Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
Characterization of the two conformations adopted by the T3 SS inner-membrane protein PrgK. (ubc.ca)

These observations recommend that thiol modifications on a number of regulatory proteins are answerable for oxidant-induced losses throughout fatigue. (ncbcs.org)

Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)

Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)

Full-length cDNA clones encoding deoxyhypusine synthase (DHS) and eucaryotic initiation factor 5A (eIF-5A) have been isolated from a cDNA expression library prepared from tomato leaves (Lycopersicon esculentum, cv. (cornell.edu)
The physical and chemical properties, protein conservative motifs, phylogenetic evolution, and expression patterns of the MADS-box transcription factors were analyzed. (bvsalud.org)

Thymidylate synthase (TS) catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) by 5, 10-methylenetetrahydrofolate (CH2H4folate) to form deoxythymidine monophosphate (dTMP) and dihydrofolate (H2folate). (rcsb.org)

Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato. (cornell.edu)

2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)

In addition to the central catalysts, CO dehydrogenase and acetyl-CoA synthase, ATPases are needed in the pathway. (degruyter.com)
Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase. (degruyter.com)

Patients must maintain a diet with limited amounts of protein (1 g/kg) and amino acid mixtures. (medscape.com)

Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins with a specific Grade. (antibodies-online.com)
Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins validated for a specific application such as ELISA, WB, SDS, AP. (antibodies-online.com)

The flow of carbon to α-acetolactate in Escherichia coli K12 is shown to involve the endogenous pool of α-ketobutyrate (α-KB). (microbiologyresearch.org)
Hybrid cluster proteins (HCPs) are Fe-S-O cluster-containing metalloenzymes in three distinct classes (class I and II: monomer, III: homodimer), all of which structurally related to homodimeric Ni, Fe-carbon monoxide dehydrogenases (CODHs). (nature.com)
We also observed that the amount of protein pHis in Escherichia coli lysates depends on carbon source and nitrogen availability in the growth medium. (princeton.edu)

Enhanced allosteric regulation of threonine deaminase and acetohydroxy acid synthase from Escherichia coli in a permeabilized cell assay system. (microbiologyresearch.org)
Allosteric interactions on biosynthetic l -threonine deaminase from E. coli K12. (microbiologyresearch.org)

Escherichia coli K-12 substr. (lbl.gov)

Measuring exact concentrations of proteins can present insights into organic processes. (ncbcs.org)

The relative levels of geranyl diphosphate synthase and farnesyl diphosphate synthase activities did not correlate with the total amount of terpenes produced, but showed some correlation with the ratio of monoterpenes to sesquiterpenes. (myspecies.info)

In a cultivar that produces mostly (R)-linalool, transcripts of (R)-linalool synthase (LIS) were the most abundant of these eight. (myspecies.info)

The nptII protein, neomycin phosphotransferase II, confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin, and was used as a selectable marker for transformed plant cells. (cbd.int)

A type III polyketide synthase then sequentially adds three acetate extender units, derived from malonyl-CoA, to a single activated 4-coumaroyl-CoA starter unit. (biomedcentral.com)

This antibody was successfully used in ELISA, western blotting, dot blot assays and immunoprecipitation and in detection and identification of histidine-phosphorylated proteins from native cell lysates when coupled with MS analysis. (princeton.edu)

Evidence that MgATP accelerates primary electron transfer in a Clostridium pasteurianum Fe protein- Azotobacter vinelandii MoFe protein nitrogenase tight complex. (degruyter.com)

Overproduction of the inactive PBP1B variants, but not of the active proteins, caused lysis of wild-type cells. (ncl.ac.uk)

Anatomy8

Organisms21

Diseases6

Chemicals and Drugs118

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes77

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Enzyme12

Biosynthesis6

Bacterial4

Genes5

Bifunctional2

Sequence6

Synthesis4

Acetolactate synthase3

Terpene synthase2

Citrate synthase4

Yeast3

Synthetase1

Molecular4

UniProt1

Acyl carrier2

Mutation2

Pyruvate-forma1

Gene3

Crystallography1

Species1

Subunit1

Enzymes5

Inhibition2

AFFINITY2

PMID1

Structural3

Metabolism2

Membrane8

Regulatory1

Sequences1

Eukaryotes1

Expression2

Thymidylate1

Characterization1

20181

Dehydrogenase2

Amino acid1

Lyase2

Carbon3

Allosteric2

Substr1

Concentrations1

Terpenes1

Transcripts1

Phosphotransferase1

Acetate1

Identification1

Evidence1

Cells1