• neurons
  • The ATP dependence of the kinetics of Ca 2+ -dependent exocytosis after flash photolysis of caged Ca 2+ was studied by capacitance measurements with submillisecond resolution in single synaptic terminals of retinal bipolar neurons. (rupress.org)
  • control
  • However, the mechanisms through which they control synaptic vesicle traffic have remained elusive. (jneurosci.org)
  • After control experiments verified that this combination of techniques is valid for the study of exocytosis in synaptic terminals, a comparison was made between the Ca 2+ dependence of the rate of exocytosis in synaptic terminals internally dialyzed with MgATP, MgATP-γ-S, or no added Mg 2+ or nucleotide. (rupress.org)
  • results
  • These results suggest a major role for MgATP in preparing synaptic vesicles for fusion, but indicate that cytosolic MgATP may have little role in events downstream of calcium entry, provided that [Ca 2+ ] i near release sites is elevated above ≈30 μM. (rupress.org)
  • events
  • These events are paralleled by a prominent increase in evoked synaptic vesicle recycling that is blocked by the specific MAPK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one. (jneurosci.org)
  • The identification of a number of highly conserved proteins that are important for membrane trafficking and fusion, combined with the development of new techniques for monitoring exocytosis have permitted models of the molecular events leading up to the calcium-dependent fusion of synaptic vesicles to be proposed. (rupress.org)