Survival nuclear mcf 7 cells. Medical search. Frequent questions

As determined using XTT cell growth and survival assays, MIH 2.4Bl exhibited growth inhibition activity on most breast cancer cell lines tested, compared with normal human mammary epithelial cells. (lsu.edu)
We also show that EphA2 overexpression is sufficient to confer malignant transformation and tumorigenic potential on nontransformed (MCF-10A) mammary epithelial cells. (aacrjournals.org)
Our previous studies revealed that the levels of protein tyrosine phosphorylation regulate a balance between cell-cell and cell-ECM adhesions in epithelial cells (7) . (aacrjournals.org)
Using oncogene-transformed mammary epithelial cells, we showed that elevated tyrosine kinase activity weakens cell-cell contacts and promotes ECM adhesions (7) . (aacrjournals.org)

Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)
In vitro data has shown that GEN can inhibit the growth of several colon cancer cells [ 8 ], while its particular effects on cancer cells and the mechanisms involved remain unknown [ 9 , 10 ]. (biomedcentral.com)
In vitro, the affinity and internalization kinetics of 111 In-R1507 were determined using the IGF-1R-expressing triple-negative breast cancer cell line SUM149. (snmjournals.org)
Flavone 2 (Luteolin-3´- O -(2´´- O - E - p -coumaroyl)-α-L-rhamnopyranoside) was responsible for the antiproliferative activity, showing in vitro cytotoxic activity against MCF-7 human cancer cell line with IC 50 value of 13.9 µM. (jmcs.org.mx)
To evaluate the function of CSE1L in vitro and in vivo , CCK-8, colony formation, cell apoptosis and subcutaneous tumor model were performed. (researchsquare.com)
The NSP 5a3a isoform had been found to be highly expressed in-vitro in particular cancer cell lines while very low to un-detectable levels in normal body tissues [1]. (oncotarget.com)
H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
In addition, Shen et al ( 14 ) reported that the autophagy of rat AF cells was induced by serum deprivation in vitro and that IL-1β upregulated serum deprivation-induced autophagy in a dose-dependent manner. (spandidos-publications.com)

Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)
Cell lifestyle Triple-negative breasts cancer tumor (MDA-MB-231 and CRL-2321) and regular mammary epithelial (MCF10A) cell lines had been extracted from the American Type Lifestyle Collection. (unambitiousus.com)

The cytotoxic effect of MIH 2.4Bl on breast cancer cell lines was determined by XTT and crystal violet assays, flow cytometry analysis, electron microscopy characterization, and terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) apoptosis assays. (lsu.edu)
To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
EMT was determined by cell invasion assays using a transwell chamber and the expression changes of EMT-related markers were confirmed by RT-PCR, Western blotting, and immunofluorescence staining. (biomedcentral.com)
The gain- and loss-of-function assays indicated that CSE1L promoted NSCLC cells proliferation and inhibits cells apoptosis. (researchsquare.com)
Relative cell viability was assessed using MTT assays. (wjgnet.com)
Failure to reverse breast cancer progression despite apparent inhibition of HER1 and HER2 kinase functions in cell-based assays as well as in patient tissues and tumours is an enigma that is not yet resolved [ 6 ]. (biomedcentral.com)
For reporter gene assays, COS-7 cells were transiently cotransfected with the plasmid pGL2P, IGF-1R or HSP90a (kindly provided by Dr. Carrie Shemanko, Calgary College, Canada) assemble and the STAT5b expression vector. (adenosine-receptor.com)

Thus, it was suggested that fucoidan can induce MCF-7 cells. (fucoxanthin.us)
Classically, HER family members function from the cell surface, where binding to cognate ligands can induce receptor homo- or hetero-dimerization with other HER family receptors [3] . (plos.org)
Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. (biomedcentral.com)
Genistein was found can induce the reversal of EMT in prostate cancer cells by an upregulated expression of epithelial marker E-cadherin and the loss of expression of mesenchymal marker vimentin [ 22 ]. (biomedcentral.com)
In this study, we demonstrated that GEN can inhibite proliferation and induce apoptosis of colon cancer cells by reversal of EMT via a Notch1/NF-κB/Slug/E-cadherin pathway. (biomedcentral.com)
CNTs can not only increase drug delivery accuracy and efficiency but also promote drug uptake, decrease drug efflux, improve tumor hypoxia conditions, and induce autophagy and apoptosis in tumor cells, which make the tumor more sensitive to antitumor agents. (eurekaselect.com)
Plumbagin, for example, was shown to inhibit the DNA-binding activity of NF-B and induce apoptosis in triple-negative MDA-MB-231 breast tumor cells (20). (unambitiousus.com)
On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)

MCF-7 cells displayed both time- and dose-dependent decreases in cell growth and survival, with a maximum cytotoxic effect observed at 72 and 96 hours. (lsu.edu)
Such compounds are attractive to researchers developing novel anti-cancer drugs, as conventional treatments - such as chemotherapy - are burdened by the therapy's cytotoxic effects on healthy cells. (technologynetworks.com)
In Trp53KO/C-MycOE mice and mice with tumors grown from Hepa1-6 cells, injection of the combination of anti-PD1 and anti-TIGIT significantly reduced tumor growth, increased the ratio of cytotoxic to regulatory T cells in tumors, and prolonged survival.PVRL1, which is up-regulated by HCC cells, stabilizes cell surface PVR, which interacts with TIGIT, an inhibitory molecule on CD8+ effector memory T cells. (stanford.edu)

Further evidence supporting the role of miR-206 in tumorigenesis originates from studies showing miR-206 is highly expressed in triple-negative breast tumors that grow independent of 17β-estradiol when compared to estrogen-receptor positive (ERα+), 17β-estradiol sensitive breast cancer cells. (wikipedia.org)
Interestingly, these 3 cell lines were derived from tumors of Luminal A origin and have ER (estrogen receptor), PR (progesterone receptor), and HER2 (human epidermal growth factor receptor 2) positive expression. (lsu.edu)
TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)
Immune checkpoint inhibitors are effective in the treatment of some hepatocellular carcinomas (HCCs), but these tumors do not always respond to inhibitors of programmed cell death 1 (PDCD1, also called PD1). (stanford.edu)
We investigated mechanisms of resistance of liver tumors in mice to infiltrating T cells.Mice were given hydrodynamic tail vein injections of clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp53 and overexpress C-Myc (Trp53KO/C-MycOE mice). (stanford.edu)
tumors were analyzed by mass cytometry using markers to detect T cells and other lymphocytes. (stanford.edu)
Tumors from mice given anti-PD1 had larger numbers of memory CD8+ T cells (CD44+CD62L-KLRG1int) and T cells that expressed PD1, lymphocyte activating 3 (LAG3), and TIGIT compared with mice not given the antibody. (stanford.edu)
Here, we demonstrate that the endoplasmic reticulum stress sensor inositol-requiring enzyme 1 (IRE1α) and its substrate transcription factor X-box-binding protein 1 (XBP1) drive NK cell responses against viral infection and tumors in vivo. (cancerindex.org)
111 In-R1507 SPECT and 89 Zr-R1507 PET images of mice with subcutaneous SUM149 tumors were acquired at 1, 3, and 7 d after injection. (snmjournals.org)
Although they account for only 10%-17% of all breast carcinomas ( 1 - 8 ), tumors are often high grade and patients are relatively young and have a reduced breast cancer-specific survival, compared with patients with estrogen receptor- or HER2 / neu -expressing tumors ( 2 , 9 ). (snmjournals.org)
Rapid growth and division of cells in tumors are correlated with intensive nucleolar metabolism as a response to oncogenic factors overexpression. (biomedcentral.com)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
Recent studies have identified overexpression of HER2 or epidermal growth factor receptor in some tumors and used this knowledge to develop successful approaches for therapeutic targeting of cancer cells (2) . (aacrjournals.org)

A number of additional miR-206 regulatory modules have been identified, such as the lncRNA HOTAIR mediated up-regulation of Bcl-w through sequestration of miR-206 which in turn enhances cellular proliferation in breast cancer cells. (wikipedia.org)
Caveolin-2 expression is necessary for the control of E2-dependent cellular proliferation of MCF-7 cells. (cusabio.com)

To identify the molecular determinants of this hormone-re- sponsive tumor phenotype , a comprehensive genome- wide analysis was performed in estrogen stimulated MCF-7 and ZR-75.1 cells by integrating time-course mRNA expression profiling with global mapping of genomic ER-alpha binding sites by chromatin immunopre- cipitation coupled to massively parallel sequencing , mi- croRNA expression profiling, and in silico analysis of transcription units and receptor binding regions iden- tified. (unica.it)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
It was found that fucoidan induced the chromatin condensation of nuclear interstitial DNA. (fucoxanthin.us)
Transcriptome and chromatin immunoprecipitation analysis revealed c-Myc as a new and direct downstream target of XBP1 for regulation of NK cell proliferation. (cancerindex.org)
This protein is thought to be involved inattaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as towhether this protein is a component of chromatin or a nuclear matrix protein. (immune-source.com)

IκBζ, an atypical member of the nuclear IκB family of proteins, is expressed at low levels in most resting cells, but is induced upon stimulation of Toll-like/IL-1 receptors through an IRAK1/IRAK4/NFκB-dependent pathway. (oncotarget.com)
GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. (biomedcentral.com)
The involvement of multiple signaling pathways (NF-κB and JNK) and proteins (EGF-R, Id-1 and Id-3) was suggested to explain the antiproliferative effect of GTT in prostate cancer (PCa) cells [ 14 ]. (biomedcentral.com)
Many laboratories have focused on the functions of the clock genes and proteins that are involved in cellular activities, including cell survival, cell cycle regulation, proliferation, apoptosis, DNA damage repair, and tumor promotion or suppression [ 5 , 6 ]. (explorationpub.com)
Export and import of mRNA and specific proteins from the nucleus is a key step in intracellular signaling and can lead to cell proliferation or apoptosis. (researchsquare.com)
Generally, these receptors explain their function on the cell membrane where, after the binding with growth factors or neuregulin undergo to homo or hetero oligomerization with the activation of the intrinsic tyrosine kinase activity and the subsequent recruitment of proteins involved in the cytoplasmic signalling pathways. (biomedcentral.com)
Malignant cells often have increased interactions with surrounding ECM 3 proteins, which provide linkages and signals that promote several aspects of metastasis (6) . (aacrjournals.org)
Scaffoldattachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind toS/MAR. The encoded protein is thought to serve as a molecular base to assemble atranscriptosome complex in the vicinity of actively transcribed genes. (immune-source.com)
Box C/D RNAs are associated with four snoRNP core proteins: NOP56, NOP58, fibrillarin, and 15.5 kDa [ 5 - 7 ]. (hindawi.com)

Fucoidan treatment of HL-60 cells could change the mitochondrial membrane permeability. (fucoxanthin.us)
Purvalanol induced mitochondrial membrane potential loss, caspase-7 and caspase-3 activation and PARP cleavage following a 48 h treatment. (spandidos-publications.com)

TNBC is considered the most aggressive subtype of breast cancer and is characterized by early recurrence, high incidence of visceral metastasis, and short survival time [ 3 ]. (nature.com)
New antitumor strategies using drugs targeted to intracellular signaling involved in cell proliferation and survival, angiogenesis, and metastasis have become promising in recent years. (researchgate.net)

Many tumor microenvironments, including lymphoma and carcinoma, are enriched in immune suppressive cells that contribute to immune exhaustion by means expression of inhibitory ligands, suppressive cytokines, and tumor-promoting factors. (researchgate.net)
Initially, there is a signaling stage, which results from various apoptotic stimuli such as DNA damage, growth factors, death receptor activation, and cytokines [ 7 ]. (oncotarget.com)
to inhibit the actions of cytokines made by Th1 immune system cells.9 Moreover, a direct impact of just one 1,25(OH)2D3 on B-cell homoeostasis Mouse monoclonal to SNAI1 has been verified.10 Therefore, supplement D may have a job in B-cell-related autoimmune disorders such as for example SLE. (informationalwebs.com)
A critical role of autophagy was identified in rat nucleus pulposus (NP) cells: Inhibition of autophagy suppresses, while activation of autophagy enhances, the catabolic effect of cytokines. (spandidos-publications.com)

These changes were characterized by an alteration of nuclear morphology and mitochondrial degeneration consistent with apoptotic cell death. (lsu.edu)
It is considered that the induction of apoptotic cell death and reversal of EMT are promising emerging strategy for prevention and treatment of cancer [ 20 , 21 ]. (biomedcentral.com)
Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)

To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells. (biomedcentral.com)
HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR). (biomedcentral.com)
In MDA-MB-231 cells, treatment by tocotrienols led to several fold increase of NRF2 expression marked by corresponding decrease in Kelch-like ECH-associated protein 1 (KEAP1) levels [ 15 ]. (biomedcentral.com)
It was thought to decrease the expression of a protein called mitochondrial transcription factor A (TFAM), a key player in cancer cell proliferation. (technologynetworks.com)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)
This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)
Herein, we also provide evidence that LOE inhibits NF-B signaling by reducing RIP1 protein levels in MDA-MB-231 cells. (unambitiousus.com)
Further, we reveal that cell routine protein and apoptotic markers, aswell as essential NF-B regulatory substances, are modulated by treatment with LOE, thus shedding light on the mechanism of actions behind the anticancer ramifications of LOE. (unambitiousus.com)
Given the constitutively low levels of hMSH4 expression in human cells [15,25], acetylation could temporally 5-HT6 Receptor Agonist review transform hMSH4 protein stability andor conformation, presumably by means of the competitors with lysine polyubiquitination-a modification known to mediate hMSH4 degradation [37]. (btkinhibitor.com)
Finally, the nuclear functions of hMSH4 and its interacting companion hMSH5 are most likely harnessed by mechanisms governing nuclear-cytoplasmic protein trafficking [46]. (btkinhibitor.com)
Thus, it will be exciting to understand whether or not hMSH4 acetylation might have any effect on nuclear-cytoplasmic protein redistribution. (btkinhibitor.com)
Using the ONCOMINE and Gene Expression Omnibus (GEO) databases, we compared the gene expression of 24 importins and exportins in 20 cancer microarray datasets and then analyzed the survival of the candidate genes, CSE1L (Chromosome Segregation Like 1 protein/CAS) was finally selected. (researchsquare.com)
Subsequent investigation of this isoform revealed its novel interaction with B23 [2], a multifunctional nucleolar protein involved in ribosome biogenesis, rRNA transcription, mitosis, cell growth control, and apoptosis [3]. (oncotarget.com)
Thus, the control of ribosome assembly and protein synthesis is essential for the survival of cancer cells. (biomedcentral.com)
In this context, during any sort of stress condition the alternative reading frame protein p14ARF subtracts MDM2, an E3 ubiquitin ligase, from the interaction with p53 in manner to free and stabilise p53 level in the nucleolus of the cell where it inhibits RNA polymerase I activity [ 3 ]. (biomedcentral.com)
Compared with CAR-T cells, CAR exosomes do not express Programmed cell Death protein 1 (PD1), and their antitumour effect cannot be weakened by recombinant PD-L1 treatment. (nature.com)
Here we report increased levels of the EphA2 (ECK) protein tyrosine kinase in clinical specimens and cell models of breast cancer. (aacrjournals.org)
Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. (knoblauchpublishing.com)
All measurements were performed in triplicate, and each and every experiment was recurring at minimum a few instances double-stranded DNA sequence was utilised as a DNA probe to bind active STAT5b and STAT3 protein in nuclear extracts. (adenosine-receptor.com)
Nuclear protein extracts ended up organized with the Nuclear Extract Package (Panomics, AY2002). (adenosine-receptor.com)
Protein concentations were calculated using the Bradford strategy.Cells had been plate on six nicely tradition dishes and incubated at 37uC, 5% CO2. (adenosine-receptor.com)
In addition, nuclear factor κB (NF‑κB), c‑Jun N‑terminal kinase (JNK), extracellular signal‑regulated kinases and p38 mitogen‑activated protein kinase inhibitors and TNF‑α were used to determine the molecular mechanism of autophagy during the inflammatory conditions, and only the NF‑κB and JNK inhibitor were found to enhance the autophagy of rat NP cells. (spandidos-publications.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
They found that apoptosis of colon cancer cells induced by fucoidan is regulated by the death of receptor-mediated apoptotic pathways. (fucoxanthin.us)
Melatonin can disrupt estrogen-mediated cellular pathways, ensuing in a lattice drop of estrogenic stimulus to the breast cancer cells. (explorationpub.com)
Apoptosis can be described as a series of tightly regulated series of molecular events that occur over three distinct stages though involve multiple and in tandem molecular pathways of activation [ 7 ]. (oncotarget.com)
Tumor suppressors, oncogenes and alternatively deregulated upstream signalling pathways can directly influence the RNA polymerase I activity inducing hyper activation of rRNA transcription in cancer cells. (biomedcentral.com)
Nucleolar activity is also influenced by the interaction between pathways activated from extracellular signals in order to coordinate ribosome synthesis and cell proliferation [ 6 ]. (biomedcentral.com)
In genomic pathways, calcitriol binds to intracellular vitamin D receptor (VDR), which subsequently heterodimerizes with another nuclear receptor retinoid X receptor (RXR). (jcancer.org)

For example, pinocembrin, a flavanone which comprises nearly 55% of the total LOE, has been shown to decrease viability and prevent epithelial-mesenchymal transition induced by TGF- in Y-79 retinoblastoma cells (25). (unambitiousus.com)
Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
Inasmuch as artificial box C/D RNAs decreased viability of transfected human cells, we propose that natural snoRNAs as well as their artificial analogues can influence the maturation of complementary pre-mRNA and can be effective regulators of vital cellular processes. (hindawi.com)
At the start of each experiment, the cells ended up resuspended in the medium at a density of 2.56105 cells/ml.Cell viability was assayed by measuring blue formazan that was metabolized from 3-(4,five-dimethylthiazol-two-yl)-two,five-diphenyl tetrazolium bromide (MTT) by AG-221 mitochondrial dehydrogenase, which is active only in dwell cells. (adenosine-receptor.com)

High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
The present study demonstrated that genistein suppressed the migration of colon cancer cells by reversal the EMT via suppressing the Notch1/NF-κB/slug/E-cadherin pathway. (biomedcentral.com)
Our previous study has proved GEN inhibits EGF-induced proliferation in colon cancer cells by promoting FOXO3 activity, targeting upstream the PI3K/Akt pathway [ 3 ]. (biomedcentral.com)
IRE1α-XBP1 were essential for expansion of activated mouse and human NK cells and are situated downstream of the mammalian target of rapamycin signaling pathway. (cancerindex.org)
RNA-seq results indicated that CSE1L activated the MAPK signaling pathway in NSCLC cells by increasing activity of P65. (researchsquare.com)
CSE1L promotes proliferation of NSCLC cells by activating the MAPK signaling pathway and inhibiting apoptosis in coordination with P65. (researchsquare.com)
These data point to the existence of an ErbB3-mediated non canonical pathway that glioblastoma cells use to control ribosomes synthesis and cell proliferation. (biomedcentral.com)
Although it is kinase defective, the HER family member HER3 can be phosphorylated by HER1 or HER2, and HER3 can couple with the pro-survival phosphatidylinositol-3-OH kinase (PI[ 3 ]K)/Akt pathway directly whereas HER1 and HER2 cannot [ 5 ] (Figure 1 ). (biomedcentral.com)

Luminal-like breast tumor cells express estrogen receptor alpha (ER-alpha) , a member of the nuclear receptor family of ligand-activated transcription factors that controls their proliferation, survival, and functional status. (unica.it)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)
Estrogen treated TG1-1 cells secreted higher levels of VEGF which were comparable to secreted levels from cells grown under hypoxic conditions. (biomedcentral.com)
Further, hypoxia and estrogen are interchangeable as both similarly modulate epithelial-endothelial cell interaction. (biomedcentral.com)
Taken together with the fact that 60-70% of human breast cancers are estrogen receptor-alpha positive[ 7 ], the evidence suggests an etiological significance of estrogen in breast cancer initiation and progression. (biomedcentral.com)

We identified 36 new putative partners for PRMT6 and we validated the interaction in vivo for 7 of them. (embl.de)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)

Long studied as a key component of the immune response, IκBζ emerges as an important regulator of inflammation, cell proliferation and survival. (oncotarget.com)

applied fucoidan to the human colon cancer DLD-1 and found that it can inhibit tumor cell multiplication by suppressing the activity of epidermal growth factors. (fucoxanthin.us)
Studies using commercially available reagents of these compounds have shown they have pro-apoptotic and anti-proliferative effects on various tumor cell lines. (unambitiousus.com)
To identify tyrosine kinases that control tumor cell adhesion, we developed novel technologies to generate monoclonal antibodies against tyrosine kinases in cancer cells (8) . (aacrjournals.org)

Three breast cancer cell lines (MCF-7, T-47D, and ZR-75-1) showed a more potent sensitivity index to growth inhibition by MIH 2.4Bl than the other breast cancer cell lines. (lsu.edu)
GTT increases the expression of several antioxidant enzymes such as thioredoxin and quinine reductase 2 (NQO2) while suppressing the growth of MCF-7 cells [ 15 ]. (biomedcentral.com)
It can prevent the growth of cancer cells along with immune-modulating and antiviral effects. (fucoxanthin.us)
first reported the impact of fucoidan on the growth of bladder cancer cells. (fucoxanthin.us)
Activation of TRPV1 by capsaicin caused significant inhibition of cancer cell growth and induced apoptosis and necrosis. (dovepress.com)
Transient receptor potential (TRP) channels, which are membranous ion channels that conduct calcium and sodium ions, have been shown to influence cancer cell growth. (dovepress.com)
10 TRPV1 agonist, capsaicin, is capable of inducing apoptosis 11 , 12 and inhibiting cancer cell growth by cell cycle arrest in many different types of cancer, for example, osteosarcoma, colon, and pancreatic cancer cells, while normal cells remained unharmed. (dovepress.com)
Cancer cells can utilize the transportins into and out of the nucleus to stimulate tumor growth and to effectively evade apoptotic mechanisms. (researchsquare.com)
We aimed to identify transportins that are overexpressed in Non-small cell lung cancer (NSCLC) and might be targeted to slow the tumor growth. (researchsquare.com)
Several members of the Epidermal Growth Factor Receptor (EGFR) family, have been identified in the nucleus and nucleolus of many cancer cells, but their function in these compartments remains unexplored. (biomedcentral.com)
Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
Interestingly, stimulation of EphA2 reverses the malignant growth and invasiveness of EphA2-transformed cells. (aacrjournals.org)
At the cellular level, malignant cells have overcome restraints on cell growth and migration that result from physical linkages and signals conveyed by cell-cell contacts (5) . (aacrjournals.org)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)

While miR-206 is tightly regulated during the embryonic development of skeletal muscle, miR-206 is also regulated by the nuclear steroid hormone receptor 17β-estradiol. (wikipedia.org)
Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
Brand TM, Iida M, Luthar N, Wleklinski MJ, Starr MM, Wheeler DL (2013) Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3. (plos.org)
Genetically engineered T cells expressing a chimeric antigen receptor (CAR) are rapidly emerging a promising new treatment for haematological and non-haematological malignancies. (nature.com)
However, it appears that regardless of the mechanism that triggers the formation of oncogenic HER2/HER3 heterodimers (HER2 over-expression or overall low HER2 but high levels of the HER3 ligand heregulin), HER3 transphosphorylation is a common response of breast cancer cells upon treatment with current inhibitors of the HER receptor tyrosine kinase network. (biomedcentral.com)

In this preliminary investigation, we wanted to observe the effect that over-expressing NSP 5a3a may have on cell cycle and its potential application in cancer treatment in aggressive cancers such as head and neck carcinomas. (oncotarget.com)
Low levels of plasma 25(OH)D 3 are associated with higher cancer incidence and mortality in men in colorectal, breast, lung and prostate cancers ( 7 - 10 ). (jcancer.org)
Soon after incubation for 15 min at area temperature in the dim, the proportion of apoptotic cells was analyzed by move cytometry (Becton-Dickinson FACScan, San Jose, CA). For positive controls 10 mM camptothecin and 23 mM actinomycin D have been utilised.Breast most cancers cells had been addressed with MSM for established moments. (adenosine-receptor.com)

mRNA microarray analyses of A549, PC14, and PC14CDDP cells that were transiently transfected with either pre-miR-197 or LNA-miR-197 and their controls. (nih.gov)

While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. (biomedcentral.com)
To investigate the effects and mechanisms of genistein on EMT of colon cancer cells, HT-29 cells were used and treated by genistein and TNF-α in this paper. (biomedcentral.com)
Genistein inhibited cell migration at 200 μmol/L. Genistein reversed the EMT of colon cancer cells by upregulation of E-cadherin and downregulation of N-cadherin, accompanied by the suppression of EMT related makers, such as Snail2/slug, ZEB1, ZEB2, FOXC1, FOXC2 and TWIST1. (biomedcentral.com)
However, current knowledge of GEN in regulating EMT of colon cancer cells is limited, and more detailed investigations of its function and mechanism are required. (biomedcentral.com)
Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)

GEN was also suggested can inhibit cell migration and invasion in both AsPC-1 and Notch-1-over-expressed AsPC-1 cells as Notch-1 could play a key role in the regulation of EMT [ 23 ]. (biomedcentral.com)
Further, trans-caryophyllene and -humulene, two sesquiterpenes present in the remove, were proven to synergize and inhibit cell development and proliferation in MCF-7 breasts cancer tumor cells (29). (unambitiousus.com)

Withaferin A (WA) causes cell death in prostate cells. (nih.gov)

Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression. (cusabio.com)
Epithelial mesenchymal transition (EMT) is an important process during tumor progression which affects critical steps of morphogenesis by interconverting epithelial cell types into cells with mesenchymal attributes [ 11 ]. (biomedcentral.com)
In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
These studies expose that LOE suppresses important features of the progression of aggressive breast cancer cells and provides a basis for further definition of its underlying mechanisms of action and anticancer potential. (unambitiousus.com)
Instead ErbB3 silencing or nucleolar stress reduce cell proliferation and affect cell cycle progression. (biomedcentral.com)
Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)

Calcitriol elicits anti-tumor effects mainly through the induction of cancer cell apoptosis, cell cycle arrest, differentiation, angiogenesis and the inhibition of cell invasiveness by a number of mechanisms. (jcancer.org)
The broad spectrum anti-tumor effects of calcitriol and analogs are mostly based on inhibition of cancer cell proliferation and invasiveness, induction of differentiation and apoptosis, and promotion of angiogenesis. (jcancer.org)

Additional analysis of cytotoxicity mediated by MIH 2.4Bl was performed using the MCF-7 cell line. (lsu.edu)
A lately study reported that hMSH4 expression in the breast cancer cell line MCF-7 was down-regulated on account of DNA hypermethylation [38]. (btkinhibitor.com)
Though, the apoptosis induced in the WI38 control cell line showed an average of a 13.2 fold increase between treated and controls comparable to the HN30 cell line 3 days post-transfection. (oncotarget.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. (nih.gov)

Long-term treatment (28 days) of human breast cancer cells (MCF-7 and SK-BR-3) and adriamycin-resistant MCF-7 cells (MCF-7/ADM) with Gd-DTPA (1 mM) or gadodiamide (GDD, 1 mM) did not affect the IC50 values of ADM. (bvsalud.org)
The antiproliferative effect of GTT in rat hepatoma dRLh-84 cells and human hepatoma Hep3B cells were reported by Sakai et al. (biomedcentral.com)
In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types. (dovepress.com)
Aung HH, Mehendale SR, Wang CZ, Xie JT, McEntee E. Cisplatin's tumoricidal effect on human breast carcinoma MCF-7 cells was not attenuated by American ginseng. (novapublishers.com)
Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
Since the hMSH4 and hMof interaction in human cells happens only following the induction of DNA harm, the basal level of hMSH4 acetylation is most likely to become maintained by acetyltransferases by means of transient interactions. (btkinhibitor.com)
In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)
We found that transfection of artificial box C/D snoRNA analogues directed to HSPA8 pre-mRNAs into human cells induced suppression of the target mRNA expression in a time- and dose-dependent manner. (hindawi.com)
Finally, IKKβ knockdown was used to further confirm the effect of the NF‑κB signal on human NP cells autophagy, and the data showed that IKKβ knockdown upregulated the autophagy of NP cells during inflammatory conditions. (spandidos-publications.com)

However, nuclear receptors are also associated with melatonin activity. (explorationpub.com)
Reports claim that sufferers with SLE possess modifications in steroid hormone fat burning capacity.11 It's been demonstrated Nifuroxazide that oestrogen results are mediated through oestrogen receptors and probably , that are expressed in an array of immune system cells and so are involved with adaptive and innate immune system responses. (informationalwebs.com)

All 1270 genes that were found to respond to 17beta-estradiol in both cell lines cluster in 33 highly con- cordant groups , each of which showed defined kinetics of RNA changes. (unica.it)
Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
Furthermore, loss of chromosomal area 1p31-32, harboring hMSH4 and various other genes, in myeloma individuals is drastically linked with shorter survival [41]. (btkinhibitor.com)
Genes to Cells. (elsevierpure.com)
In earlier studies, the development of snoRNA expression vectors was one of the main approaches to study the influence of box C/D snoRNAs on target genes in mammalian cells. (hindawi.com)

Using multi-omic approach, we discover that nuclear DNA damage promotes mitochondrial β-oxidation and drives a global loss of fat depots. (knoblauchpublishing.com)

7 , 8 These channels can be activated by many stimuli, including temperature or pH changes in the extracellular space. (dovepress.com)
Each of these stimuli can respectively activate a subsequent initiator of apoptosis or inhibitor of anti-apoptosis, such as PUMA/Noxa, Bad, Bid, and Bim [ 7 - 8 ]. (oncotarget.com)

Above mentioned effects were significantly blocked when the cells were pretreated with an antioxidant N-acetylcysteine (NAC), suggesting the involvement of ROS generation. (biomedcentral.com)

In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)
When malignant cells metastasize to distant sites in the body, morbidity and mortality increase significantly (3) . (aacrjournals.org)
Background Loss of function of fumarate hydratase (FH), the mitochondrial tumor suppressor and tricarboxylic acid (TCA) cycle enzyme, is associated with a highly malignant form of papillary and collecting duct renal cell malignancy. (immune-source.com)

Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
Cells had been lysed on ice for 10 min in radioimmunoprecipitation assay (RIPA) lysis buffer made up of protease and phosphatase inhibitors. (adenosine-receptor.com)

miR-206 is studied in C2C12 myoblast cells as this is a widely used model for the study of cellular differentiation of skeletal muscle. (wikipedia.org)
Generally, the process of apoptosis is a critical and essential cellular function allowing regulated turn-over of cells during embryonic development, differentiation, and in response to disease [ 5 - 6 ]. (oncotarget.com)

Prx exists as six unique isoforms (PrxI to VI) in mammalian cells. (biomedcentral.com)

This indolamine advances apoptosis, which arrests the cell cycle and regulates metabolic activity. (explorationpub.com)
Typically, this complex process which regulates cellular homeostasis is characterized by distinct morphological and biochemical signatures including nuclear condensation, cell shrinkage, formation of apoptotic bodies, cellular blebbing and the externalization of phosphatidylserine [ 5 - 6 ]. (oncotarget.com)

Pvrl1 and Pvrl3 were knocked down in Hepa1-6 cells by using short hairpin RNAs. (stanford.edu)
Small nucleolar RNAs (snoRNA) are commonly known to be involved in the processing of precursor ribosomal RNA (pre-rRNA) and small nuclear RNAs (snRNAs). (hindawi.com)

In this study, stimulation by the TRPV1 agonist, capsaicin, of SUM149PT cells, a model system for the most aggressive breast cancer subtype, triple-negative breast cancer, led to intracellular calcium signals that were diminished by the specific TRPV1 antagonist, capsazepin. (dovepress.com)
Typically, CARs consist of a target binding domain, which is an extracellular domain that is specifically expressed by CAR-T cells, a transmembrane domain, and a signalling domain, which is an intracellular domain that provides an activation signal to T cells. (nature.com)
For the intracellular metabolomic analysis, cells were quickly washed three instances with PBS to remove contaminations from the metabolites in the press. (immune-source.com)
Ma et al ( 15 ) revealed that compression activated autophagy in NP cells and that compression-induced autophagy was closely associated with intracellular reactive oxygen species production. (spandidos-publications.com)

Further, halofuginone inhibited the nuclear localization of NF-B and AP1, which are essential transcriptional activators of matrix metalloproteinase-9 (MMP-9), therefore reducing migration and invasiveness of these cells (21). (unambitiousus.com)

Increased PVRL1 was associated with shorter times of disease-free survival. (stanford.edu)
Results: 7 transportins were over-expressed in NSCLC patients, and higher expression of CSE1L and XPO5 are associated with shorter survival times. (researchsquare.com)
Increased expression of CSE1L in tumor tissues associated with shorter survival times and advanced pathological stage of patients with cancer. (researchsquare.com)

Effects via Activation of Dendritic Cells and Regulatory T Cells. (novapublishers.com)

19 ] suggested that tocotrienol rich fraction directly influenced the expression dynamics of peroxiredoxin-2 in H 2 O 2 challenged lymphocyte cells to improve the cells ability to resist oxidative damage. (biomedcentral.com)

Next, we identified full-length nuclear HER3 association and regulation of a 122 bp region of the cyclin D1 promoter. (plos.org)
Apoptosis, which is a major way of programmed cell death, has been known to all plays an important role in the regulation of tissue development and homeostasis [ 17 ]. (biomedcentral.com)
With the synchronized output of the central circadian clock in relation to the pineal gland and other peripheral oscillators, melatonin acts as a key role player in the regulation of intermediary metabolism and cancer prevention [ 7 ]. (explorationpub.com)
Melatonin has oncostatic properties that are mediated by induction of apoptosis, arresting the cell cycle, regulation of metabolic activity, and the inhibition of hypoxia-inducible factor 1α (HIF-1α) activity [ 9 ]. (explorationpub.com)

In current clinical development, targeting CD19 + B cell malignancies, which include acute and chronic B-cell leukaemia and B-cell non-Hodgkin lymphomas (NHLs), with anti-CD19 CAR-T cells is one of the most advanced adoptive T-cell therapies and has been approved by the FDA. (nature.com)
Although a strong response was observed for CAR-T cells in patients with treatment-refractory haematologic malignancies, only modest outcomes have been reported in solid tumours. (nature.com)

In the eukaryotic cells the production of ribosomes takes place in the nucleolus, a specialized subnuclear compartment where the RNA Polymerase I (RNA Pol I) catalyses the ribosomal RNA synthesis (rRNA). (biomedcentral.com)

However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)

However, treatment with Gd-DTPA or GDD significantly increased TRPC5 expression and decreased the accumulation of ADM in the nuclei of MCF-7 and SK-BR-3 cells, promoting the survival of these two breast cancer cells in the presence of ADM. (bvsalud.org)
These data indicate that prolonged GBCA treatment may lead to increased breast cancer cell survival owing to the upregulation of TRPC5 expression and the increased ADM resistance. (bvsalud.org)
Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment. (biomedcentral.com)
18 ]. They reported that the expression of Prx1 and Prx6 is selectively regulated in alveolar type II cells isolated from vitamin E-depleted rats, depending on the severity of oxidative load. (biomedcentral.com)
In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. (cusabio.com)
Moreover, genistein can inhibit the expression of notch-1, p-NF-κB and NF-κB, while promote the expression of Bax/Bcl-2 and caspase-3 in HT-29 cells. (biomedcentral.com)
The expression level of CSE1L was detected in both NSCLC cells and clinical samples. (researchsquare.com)
The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). (knoblauchpublishing.com)
EMSA experiment is performed by incubating a biotin-labeled transcription component (TF-STAT5 and STAT3) probe with handled and untreated nuclear extracts.The expression vectors for mouse STAT5b (pMX/STAT5b kindly provided by Dr. Koichi Ikuta, Kyoto University, Japan) had been built as earlier explained. (adenosine-receptor.com)

However, the autophagy induced by TNF‑α and IL‑1β and the corresponding molecular mechanism appear to be cell‑type dependent. (spandidos-publications.com)

The bioactive compound of Kencur ginger, ethyl p-methoxycinnamate, reduces cancer cell proliferation in mice. (technologynetworks.com)
In mice, this compound reduced cancer cell proliferation at low doses. (technologynetworks.com)

in particular, HER3 signalling buffered against incomplete inhibition of HER2 kinase activity has been suggested to be the mechanism that allows HER2 over-expressing breast cancer cells to escape HER TKIs. (biomedcentral.com)

Constitutive nuclear factor-B (NF-B) signaling offers been shown to contribute to the heightened inflammatory state and survival in TNBC cells. (unambitiousus.com)

The migration of multiple myeloma cells which are treated with chemotherapy drugs was affected by fucoidan. (fucoxanthin.us)

Moreover, CAR-T cells are vulnerable to immunosuppressive mechanisms. (nature.com)
While the mechanisms of tumorigenesis in FH-deficient cells have been extensively looked into, the metabolic changes caused by the loss of FH activity have only been partially tackled. (immune-source.com)

Antitumor therapies targeted to reduce the induction, recruitment, or suppressive activities of the immune cells have been investigated. (researchgate.net)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)

Anatomy12

Organisms11

Diseases45

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment76

Phenomena and Processes43

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science9

Named Groups3

Health Care34

Geographicals1

Epithelial cells4

Vitro8

Mammary2

Assays7

Induce9

Cytotoxic3

Tumors13

Cellular proliferation2

Chromatin5

Proteins9

Mitochondrial membrane2

Metastasis2

Cytokines4

Apoptotic cell5

Protein23

Pathways8

Viability4

Pathway8

Estrogen5

Vivo2

Proliferation and survival1

Tumor cell3

Growth14

Receptor5

Cancers3

Transiently1

Colon8

Inhibit cell2

Prostate1

Progression6

Anti-tumor2

Line5

Human9

Receptors2

Genes5

Promotes1

Stimuli2

Antioxidant1

Malignant3

Inhibitors3

Differentiation2

Mammalian1

Regulates2

RNAs2

Intracellular4

Transcriptional1

Associated with shorter3

Dendritic Cells1

Oxidative damage1

Regulation4

Malignancies2

Eukaryotic cells1

Interaction1

Expression9

Molecular mechanism1

Cancer cell proliferation2

Kinase1

Inflammatory1

Migration1

Mechanisms2

Induction2