Surface biofilms. Medical search. Frequent questions

To help prevent biofilms from forming on the surfaces of these devices, researchers at Harvard's School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering have developed a new coating technology that sheds any bacteria forming on it. (medgadget.com)
The oil prevents bacteria sticking to it, and since it's constantly seeping out the device's surface maintains its slippery and repulsive nature. (medgadget.com)
Zobell ( 2 ) observed that the number of bacteria on surfaces was dramatically higher than in the surrounding medium (in this case, seawater). (cdc.gov)
We applied this strategy to study coagulase (Coa), a secreted protein and a major contributor to the formation of a fibrin network in S. aureus biofilms that protects bacteria from the host immune system and increases attachment to host surfaces. (sdu.dk)
This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
Because biofilms protect the bacteria, they are often more resistant to traditional antimicrobial treatments, making them a serious health risk. (wikipedia.org)
Dispersion forces between the polymer chains and the bacterial cells prevent bacteria from binding to the surface and initiating biofilm growth. (wikipedia.org)
Biofilms form as a way of survival for bacteria in aqueous situations. (wikipedia.org)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
Based on the comparison of clean water and dirty water control bath toys, we argue that bath toy biofilms are influenced by (1) the organic carbon leaching from the flexible plastic material, (2) the chemical and biological tap water quality, (3) additional nutrients from care products and human body fluids in the bath water, as well as, (4) additional bacteria from dirt and/or the end-users' microbiome. (discovermagazine.com)
Once there's a quorum or a sufficient number of these bacteria, a structure, a three-dimensional structure, rises up off the surface and forms the channels and the architecture that we call mature biofilm. (hstalks.com)
Bacteria on damp and dry surfaces do not remain as isolated and free-floating life forms. (infectioncontroluniversity.com)
All biofilms harbored gram-positive bacteria, including pathogens associated with HAIs. (infectioncontroluniversity.com)
Bacteria embedded in biofilms can be up to 1,000 times more resistant to disinfectants. (infectioncontroluniversity.com)
Cleaning with surfactant-based detergents or disinfectants deposits organic residues, which some bacteria can metabolize as a nutrient source for growth in biofilms. (infectioncontroluniversity.com)
IMPORTANCE Most bacteria express multiple adhesins that contribute to surface attachment and colonization. (nih.gov)
In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)
Bacteria within biofilms are protected from external damage and attacks from the host immune system and can exchange genomic information including antibiotic-resistance genes. (brad.ac.uk)
Understanding how bacteria develop biofilms is pertinent to tackle biofilm-associated infections and transmission. (brad.ac.uk)
A biofilm is a collection of bacteria and/or fungi that exists in a multicellular or community form, encased in an extracellular polysaccharide matrix that they themselves synthesise. (thepigsite.com)
Formation of a biofilm starts with adherence of bacteria followed by production of the polysaccharide matrix. (thepigsite.com)
Other bacteria, other micro-organisms and debris become incorporated in the biofilm. (thepigsite.com)
Bacteria within a biofilm communicate by quorum sensing leading to phenotypic modifications and changes in gene expression. (thepigsite.com)
Bacteria within the biofilm are protected from disinfectants and have a fundamentally reduced antibacterial sensitivity compared with planktonic, free-living, bacteria. (thepigsite.com)
5.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. (astm.org)
RESULTS AND CONCLUSION: (1) Bacteria were inoculated first and then macrophages were added to the experiment: CFU cell count, flow cytometry technology and inverted fluorescence microscope detection showed that as the surface hardness of the material increased, the phagocytosis of macrophages increased and the number of E. coli biofilm cells decreased. (syr.edu)
2) Macrophages were inoculated first and then bacteria were added to the experiment: CFU cell count test showed that as the surface hardness of the material increased, the phagocytosis of macrophages increased and the number of bacterial cells decreased. (syr.edu)
Even though biofilm cells showed clear changes in gene expression compared to planktonic cells, no differences could be detected between bacteria on SLIPS and on control surfaces. (nanosurf.com)
Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
A biofilm is an aggregate of surface-associated bacteria, and the biofilm matrix is the envelope of polymeric substances that surrounds the bacteria. (planteome.org)
Our solution addresses a pervasive need to design environmentally friendly materials to impede dangerous surface bacteria growth," the BGU researchers from the Avram and Stella Goldstein-Goren Department of Biotechnology Engineering explain. (eurekalert.org)
This holds tremendous potential for averting biofilm formed by surface-anchored bacteria and could have a tremendous impact. (eurekalert.org)
In the scope of this, the interaction between the surfaces and bacteria, which is highly determined by topography and surface chemistry, will be investigated. (sckcen.be)
Scientists in Austria have investigated where biofilms hide and what bacteria can be found in them. (foodsafetynews.com)
Researchers isolated bacteria from the biofilm-positive samples and characterized them. (foodsafetynews.com)
This clearly shows that these are multi-species biofilms, so different bacteria colonize the same biofilm. (foodsafetynews.com)
Most often we were able to assign the biofilm bacteria of the genera Brochothrix, Pseudomonas and Psychrobacter. (foodsafetynews.com)
When bacteria team up in sticky communities called biofilms, they can be nearly impossible for conventional antibiotics to eradicate. (technologyreview.com)
MIT researchers are developing viruses to combat biofilms like this one, a group of bacteria growing inside a catheter. (technologyreview.com)
Working with Boston University synthetic biologist James Collins, -Harvard-MIT graduate student Timothy Lu has designed a new, highly effective means of dispersing and killing the bacteria living in biofilms. (technologyreview.com)
The two researchers have engineered viruses that attack both the bacteria and the carbohydrates that hold them together-eliminating, for example, more than 99 percent of the E. coli in a model biofilm. (technologyreview.com)
If such bacteria-attacking viruses prove safe for industrial and clinical use, researchers could develop stocks of different kinds of viruses, each tailored to attack a different kind of biofilm. (technologyreview.com)
Because they are protected by a sticky carbohydrate scaffold called a matrix, bacteria living communally in biofilms are a thousand times as resistant to antibiotics as free--swimming bacteria are, says Collins. (technologyreview.com)
In the BacData project, the researchers are investigating two clinically highly relevant biofilm associated infections: the lung infection in cystic fibrosis patients caused by Pseudomonas bacteria and the implant infection as an example of frequently occurring multi-species biofilm infections. (l3s.de)
In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than can cause infections in humans, especially in hospitals. (netbalaban.net)
The role of volatile sulfur compounds (VSCs) in producing bacteria that colonize the dorsal surface of the tongue was recently understood as a primary cause of halitosis (bad breath). (medscape.com)
Under a high magnification, this scanning electron microscopic (SEM) image depicted numbers of Staphylococcus aureus bacteria, which were found on the luminal surface of an indwelling catheter. (cdc.gov)
This biofilm has been found to protect the bacteria that secrete the substance from attacks by antimicrobial agents such as antibiotics. (cdc.gov)
Although microorganisms can live free in the environment, most bacteria are associated in a biofilm. (bvsalud.org)
Not only the teeth, but also the restorative material can be colonized by bacteria and allow biofilm growth 3,7,12 . (bvsalud.org)
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. (bvsalud.org)
In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. (bvsalud.org)
Bacteria and microbes mainly grow on the inside of our water pipes, which can be seen in the form of a thin, sticky coating - a so-called biofilm. (lu.se)

Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
Rediscovery of a microbiologic phenomenon, first described by van Leeuwenhoek, that microorganisms attach to and grow universally on exposed surfaces led to studies that revealed surface-associated microorganisms (biofilms) exhibited a distinct phenotype with respect to gene transcription and growth rate. (cdc.gov)
These biofilm microorganisms have been shown to elicit specific mechanisms for initial attachment to a surface, development of a community structure and ecosystem, and detachment. (cdc.gov)
Van Leeuwenhoek, using his simple microscopes, first observed microorganisms on tooth surfaces and can be credited with the discovery of microbial biofilms. (cdc.gov)
Heukelekian and Heller ( 1 ) observed the "bottle effect" for marine microorganisms, i.e., bacterial growth and activity were substantially enhanced by the incorporation of a surface to which these organisms could attach. (cdc.gov)
5 ) in 1978 put forth a theory of biofilms that explained the mechanisms whereby microorganisms adhere to living and nonliving materials and the benefits accrued by this ecologic niche. (cdc.gov)
Biofilm formation occurs when free floating microorganisms attach themselves to a surface. (wikipedia.org)
Aggregate of microorganisms in which cells that are frequently embedded within a self-produced matrix of extracellular polymeric substances (EPSs) adhere to each other and/or to a surface. (wikipedia.org)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Biofilms on highly touched surfaces pose a risk to patients because dry-surface biofilms persist for long periods, and microorganisms within biofilm have been shown to be transmitted. (infectioncontroluniversity.com)
Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. (frontiersin.org)
Biofilms are composed of microorganisms attached to a solid surface or floating on top of a liquid surface. (nature.com)
Usually, these electroactive microorganisms form an electroactive biofilm (EAB) on the electrode surface. (uni-bremen.de)
Biofilms are microorganisms that cling to a surface in a very narrow space and form a protective layer, the so-called matrix. (foodsafetynews.com)
Biofilms were analyzed regarding the number of viable microorganisms (CFU/mL), the metabolic activity (MTT assay) and the proportion of hyphae ( optical microscopy ). (bvsalud.org)
They prevent the adherence of microorganisms which can stick to the mucosa or tooth surfaces. (news-medical.net)
Microorganisms present in the oral cavity form a layer on the oral surfaces. (news-medical.net)
Besides s treptococci, various other microorganisms can also form biofilms at varied sites. (news-medical.net)
Bacterial biofilm can be simply defined as a set of microorganisms and their extracellular products, adhered on many surface types. (bvsalud.org)

Specifically, 10 μm tall hexagon-shaped topographic patterns with side length of 15 μm and interpattern distance of 2 μm were prepared to reduce biofilm formation on the side of protruding patterns and interrupt cell-cell interaction in the grooves. (syr.edu)
Knowledge gained on presence and composition, published in the International Journal of Food Microbiology , could help to prevent and reduce biofilm formation within food processing environments. (foodsafetynews.com)
Introduction: Currently, new methods to reduce biofilm formation on biomaterials are very studied, for example the use of silver nanoparticles, which were bactericidal. (bvsalud.org)

As a demonstration, we show the preparation of silicone-coated polyurethane catheters and significant reduction of Escherichia coli and Staphylococcus epidermidis biofilm formation on the catheter surface. (medgadget.com)
Staphylococcus aureus is a major human pathogen that utilises many surface-associated and secreted proteins to form biofilms and cause disease. (sdu.dk)
The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus ( S. aureus ) growth and prevented it from forming biofilms. (frontiersin.org)
Staphylococcus-aureus dry-surface biofilms are not killed by sodium hypochlorite. (infectioncontroluniversity.com)

With increasing flow rates, the surface exhibited a significant reduction in attached biofilm of both the oral initial colonizer Streptococcus oralis and an oral multispecies biofilm composed of S. oralis, Actinomyces naeslundii, Veillonella dispar, and Porphyromonas gingivalis. (nanosurf.com)
Microscopic evaluation of these biofilms indicated that streptococcus accounts for approximately 80 percent of this colonization. (news-medical.net)
The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group a Streptococcus. (cdc.gov)
Objective: This study aimed to compare in vitro the Streptococcus mutans biofilm formation on conventional light-cured composite resin with that on experimental light-cured composite resin, modified with silver nanoparticles. (bvsalud.org)

The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
Because of this, it is crucial to understand how LAB-CFS affects the makeup and function of metabolites in S. aureus biofilms. (frontiersin.org)
No resistance mutations occurred, implying that hypochlorite resistance is an intrinsic property of S. aureus biofilms. (infectioncontroluniversity.com)

The presence of dry biofilms harboring bacterial pathogens is virtually universal on commonly used items in healthcare settings," according to the study. (infectioncontroluniversity.com)
Dry-surface biofilms are widespread and can incorporate pathogens and multi-drug resistant organisms (MROs). (infectioncontroluniversity.com)
Biofilms may include spoilage agents and food pathogens such as Listeria, E. coli and Salmonella. (foodsafetynews.com)

Antibiotics, biocides, and ion coatings are commonly used chemical methods of biofilm prevention. (wikipedia.org)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
Biofilms are notoriously resistant to antibiotics. (sciencedaily.com)
Microbial biofilms are of special concern, because they can cause damage to spaceflight equipment and are difficult to eliminate due to their increased resistance to antibiotics and disinfectants. (sckcen.be)
Large doses of antibiotics can usually eradicate these infections, but there is some worry that drug--resistant biofilm infections are becoming more common. (technologyreview.com)
What's more, some evidence suggests that the use of antibiotics actually induces biofilm formation. (technologyreview.com)
Biofilms make easy to obtain nutrients and increase the resistance to antibiotics 10 . (bvsalud.org)

Two major thrusts in the last decade have dramatically impacted our understanding of biofilms: the utilization of the confocal laser scanning microscope to characterize biofilm ultrastructure, and an investigation of the genes involved in cell adhesion and biofilm formation. (cdc.gov)
As an important surface property, topography has been shown to affect bacterial adhesion and biofilm formation. (syr.edu)
Bacterial adhesion and biofilm formation are affected by the characteristics of biological materials, such as surface charge, hydrophobicity, hardness, and surface chemistry. (syr.edu)

Analysis of a flow culture of Pseudomonas aeruginosa through untreated PDMS and iPDMS tubing shows at least an order of magnitude reduction of biofilm formation on iPDMS, and almost complete absence of biofilm on iPDMS after a gentle water rinse. (medgadget.com)
This topography exhibited 85% and 46% reduction of biofilm formation and associated conjugation, respectively, compared to the smooth control. (syr.edu)

abstract = "Bacterial biofilms are highly tolerant to antimicrobials and play an important role in the development and spread of antibiotic resistance based on horizontal gene transfer due to close cell-to-cell contact. (syr.edu)
abstract = "Aquaculture settings present a number of surfaces that can potentially act as a reservoir for Flavobacterium psychrophilum, resulting in a serious threat to the health of farmed salmonids. (pucv.cl)

Much of the work in the last 2 decades has relied on tools such as scanning electron microscopy (SEM) or standard microbiologic culture techniques for biofilm characterization. (cdc.gov)
The present study gives a detailed characterization of bath toy biofilms and a better understanding of determinants for biofilm formation and development in systems comprising plastic materials in contact with potable water. (discovermagazine.com)
In this project, magnetic resonance imaging (MRI) is applied for the characterization of electroactive biofilms in porous electrodes. (uni-bremen.de)
Research projects for this candidate will involve the development of functional materials for medical applications, including but not limited to systematic materials characterization, surface charge measurements, and quantification of interactions (biomechanics) on biointerface. (aeesp.org)
Preference will be given to candidates who have experience in performing functional materials synthesis and characterization, biofilm growth and analysis, as well as the experience in the journal publication. (aeesp.org)

Bacterial biofilms are a cause of countless challenging infections originating from implanted medical devices, urinary and intravenous catheters, and other clinical tools that interface with the human body. (medgadget.com)
Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
For example, there are more than one million cases of catheter-associated urinary tract infections (CAUTI) reported each year, many of which can be attributed to bacterial biofilms. (wikipedia.org)
Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
More than 65 percent of hospital-acquired infections manifest as biofilms. (sciencedaily.com)
A study published in the Journal of Hospital Infection examined occurrence of dry biofilms on hospital surfaces, as well as the effect they might have on the spread of hospital-acquired infections (HAIs). (infectioncontroluniversity.com)
In clinical settings, persistent presence of biofilms increases the risks of healthcare-associated infections and imposes huge healthcare and economic burdens. (brad.ac.uk)
Dispersed bacterial cells from attached biofilms on medical devices or host tissues may also serve as the origin of further infections. (brad.ac.uk)
BACKGROUND: Totally 60%-70% of hospital-acquired infections are related to medical devices of different materials, and bacterial biofilm cells play an important role in chronic infections. (syr.edu)
To combat implant-associated infections, there is a need for novel materials which effectively inhibit bacterial biofilm formation. (nanosurf.com)
Biofilms, which often form on hard surfaces such as catheters and water pipes, can cause gingivitis and chronic ear infections. (technologyreview.com)
Biofilm-associated infections are therefore one of the greatest challenges facing medicine . (l3s.de)
BacData is intended to improve the prognosis of patients suffering from biofilm-associated infections in the long term and, in addition, to create the basis for a personalised therapy of further infectious diseases. (l3s.de)
Fungal infections in the oral cavity can be a result of the growth of fungal biofilms. (news-medical.net)
And while it isn't known whether biofilms have directly contributed to astronaut illnesses, on Earth, biofilms are associated with 65% of microbial infections, and 80% of chronic infections, the researchers say. (netbalaban.net)
Three recent articles [ 1 , 2 , 3 ] highlight how our understanding of bacterial persistence and recent advances in disinfection modalities may provide a means for control of biofilms and nosocomial infections. (medscape.com)

Materials and Methods Candida albicans biofilms on acrylic resin plates were treated for 15 minutes with either nystatin, fluconazole, Polident, 220µM erythrosine + 1% (w/w) nano-TiO 2 + 15 J/cm 2 blue light photodynamic therapy (Ery PDT), or distilled water. (thieme-connect.de)
At 6 hours, Ery PDT reduced the number of viable C. albicans in biofilms by 0.28log 10 CFU/mL, which was equal to the effect of fluconazole and Polident. (thieme-connect.de)
Conclusion Photodynamic therapy with erythrosine + nano-TiO 2 + blue light at low light power density (15 J/cm 2 ) was as effective at inhibiting C. albicans biofilm on acrylic resin as fluconazole and Polident. (thieme-connect.de)
however, biofilm-associated C. albicans can be resistant to these agents. (thieme-connect.de)
Red propolis hydroalcoholic extract inhibits the formation of Candida albicans biofilms on denture surface. (bvsalud.org)
To evaluate the antifungal activity of the red propolis hydroalcoholic extract (RPHE) against Candida albicans biofilms . (bvsalud.org)
C. albicans biofilms were formed on the surface of resin specimens preconditioned with artificial saliva (1h). (bvsalud.org)
RPHE has antifungal activity against C. albicans biofilms , suggesting its use for the biofilm control on denture surfaces. (bvsalud.org)
C. Albicans are naturally occurring fungi in the oral cavity but in the presence of unhygienic conditions, it quickly multiplies and grows especially on the hard surfaces like dentures. (news-medical.net)
The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. (lu.se)
These compounds, benzyl α-l-fucopyranoside and benzyl β-d-xylopyranoside, inhibit the hyphae formation and adhesion of C. albicans to a polystyrene surface, resulting in a reduced biofilm formation. (lu.se)

The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)

Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
These attached communities are known as biofilms. (thepigsite.com)

Water purification methods are being scrutinized here because it is in this state that contamination is thought to occur and biofilms are formed. (wikipedia.org)
Biofilms are proven sources of contamination in the food industry. (foodsafetynews.com)
If food comes into contact with these biofilms, contamination may occur. (foodsafetynews.com)
We discovered further biofilms in drains and water hoses - places that are not cleaned by default, but are a potential source of contamination. (foodsafetynews.com)
Alfa and colleagues [ 2 ] looked at the impact of improper positioning of the elevator lever of a duodenoscope on bacterial persistence, biofilm formation, and endoscope contamination. (medscape.com)

By using a specific polysaccharide-stain called Ruthenium red and coupling this with osmium tetroxide fixative, these researchers were also able to show that the matrix material surrounding and enclosing cells in these biofilms was polysaccharide. (cdc.gov)
Most microbes live in mixed communities and in many circumstances, these are attached to an environmental surface encasing themselves in an extracellular polysaccharide matrix which they themselves have produced. (thepigsite.com)
Biofilms, a complex aggregation of cells embedded in a polysaccharide matrix, have been of interest for a long time in history - right when Antoine van Leuwenhoek examined a scraping of his tooth plaqueunder a microscope that he had built 1 . (nature.com)
2016. Novel Anti-Adhesive Biomaterial Patches: Preventing Biofilm Using Metal Complex Films (MCF) Derived from a Microalgal Polysaccharide. (eurekalert.org)
The absence of either OxyR or PNAG1 resulted in enhanced biofilm formation, higher surface hydrophobicity, and increased motility, implying that K-locus-driven polysaccharide production in both the oxyR and PNAG1 deletion mutants may be related to these phenotypes. (korea.ac.kr)

Control of biofilms is difficult and addresses preventing initial formation and removing existing biofilms. (thepigsite.com)
Kober-Rychli added further research is needed in the prevention, rapid detection and control of biofilms in the food sector. (foodsafetynews.com)

Scientists from the Feed and Food Quality, Safety and Innovation (FFoQSI) Competence Center examined 11 rooms in November 2018 within the plant and took 108 samples - 47 food contact surfaces before operation and 61 surfaces not directly in contact with food during daily operation - for the presence of biofilms. (foodsafetynews.com)

Conclusion: The risk may be further exacerbated by inefficient cleaning and disinfection practices for hospital surfaces. (infectioncontroluniversity.com)
In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)
These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks. (frontiersin.org)
Seven of the biofilm-positive samples were taken during work and three after cleaning and disinfection, including one at a screw conveyor. (foodsafetynews.com)

[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
This matrix, which consists of carbohydrates, proteins and extracellular DNA, protects inhabitants of the biofilm from external influences such as disinfectants, UV radiation and dehydration. (foodsafetynews.com)

The results demonstrate that square-shaped topographic patterns with side length of 20, 50, and 100 μm and interpattern distance equal to or larger than 10 μm promote biofilm formation and conjugation compared to the smooth control. (syr.edu)
The samples were incubated in bacterial suspension (S. mutans) enriched with 20% sucrose to promote biofilm growth on the surfaces. (bvsalud.org)

Effect of surface hardness of biomaterials in the phagocytosis of e. (syr.edu)
Dive into the research topics of 'Effect of surface hardness of biomaterials in the phagocytosis of e. (syr.edu)
According to the research published in Advanced Materials Interfaces , anti-adhesive patches that are developed from naturally occurring biomaterials can prevent destructive bacterial biofilm from forming on metal surfaces when they are immersed in water and other damp environments. (eurekalert.org)
The successful candidate will have a unique opportunity to develop and expand upon a diversified technical skill set while making a positive contribution to the areas of biomaterials, biomechanics, and biofilms. (aeesp.org)

So the detachment fragments have all the colony defenses of the mature biofilm. (hstalks.com)
Biofilms have been suggested as a continuum of growth modes for adapting to different environments, initiating from bacterial cells sensing their attachment to a surface and then switching cellular physiological status for mature biofilm development. (brad.ac.uk)

The goal in biofilm research and efficacy testing is to choose the growth reactor that generates the most relevant biofilm for the particular study. (astm.org)

3 ) used scanning and transmission electron microscopy to examine biofilms on trickling filters in a wastewater treatment plant and showed them to be composed of a variety of organisms (based on cell morphology). (cdc.gov)
Among other things, a technology to examine biofilms in water pipes with PCR-based large-scale sequencing has been developed together with the Swedish National Forensics Centre and Lund researchers Anders Tunlid, Björn Canbäck and Tomas Johansson, who used a similar technique to examine the microflora in soil samples. (lu.se)

the means of prevention have thus concentrated largely on two areas: killing the microbes that form the film, or preventing the adhesion of the microbes to a surface. (wikipedia.org)
A biofilm is any group of microbes that stick together on a surface. (sciencedaily.com)
Inside these vials are chambers containing the new surface material and the microbes. (netbalaban.net)
One approach to preventing biofilms is to use surfaces coated with certain metals or oxides that kill microbes, but this approach can fail when a layer of dead microbes builds up on the surface and allows biofilm to form above it. (netbalaban.net)
But this was not the case with the liquid-infused surface that performed well in the ISS experiments: Rather than killing the microbes, it prevented them from adhering to the surface in the first place. (netbalaban.net)
This spiky surface is then infused with a silicon oil, which is drawn into the texture and held in place by capillary action, leaving a smooth and highly slippery surface that significantly reduces the adhesion of microbes and prevents them from forming a biofilm. (netbalaban.net)
Periphyton is a complex mixture of algae , cyanobacteria , heterotrophic microbes , and detritus that is attached to submerged surfaces in most aquatic ecosystems . (wikipedia.org)

Unsurprisingly, they found fungi (mold), as well as 'dense biofilms with complex bacterial and fungal communities. (discovermagazine.com)
Denture stomatitis is a condition caused primarily by fungal growth on all surfaces of dentures that are worn for prolonged times and/or following inadequate cleaning of dentures. (thieme-connect.de)
Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people. (netbalaban.net)
The organisms are commonly bacterial (possibly as part of a biofilm on the mucosal surface) but may be fungal. (msdmanuals.com)

On prolonged incubation, S. aureus regrew and formed biofilms. (infectioncontroluniversity.com)
After overnight incubation, biofilms were daily exposed (2x/day for 15 min) along 3 days with 3% RPHE, 0.12% chlorhexidine (CHX), 50,000 IU/mL nystatin (NYS) or saline ( 0.9% NaCl ). (bvsalud.org)
After a 72 h incubation period, F. psychrophilum had formed mature biofilms composed by dense, multilayered clusters of short bacilli on stainless steel, polystyrene, and polycarbonate. (pucv.cl)

The introduction of antimicrobial surfaces for medical, pharmaceutical and industrial purposes has shown a unique potential for reducing and preventing biofilm formation. (sckcen.be)
This article describes the development process of ESA's BIOFILMS experiment, that will evaluate biofilm formation on various antimicrobial surfaces under spaceflight conditions. (sckcen.be)
This article describes the development process of ESA{\textquoteright}s BIOFILMS experiment, that will evaluate biofilm formation on various antimicrobial surfaces under spaceflight conditions. (sckcen.be)

Ozone targets extracellular polysaccharides, a group of bacterial colonies on a surface, and cleaves them. (wikipedia.org)
Using a microfluidic device, an increased amount of polysaccharides and enhanced biofilm formation were observed following continuous exposure to H 2 O 2 and removal of the H 2 O 2 -sensing key regulator, OxyR, in Acinetobacter oleivorans DR1 cells. (korea.ac.kr)

For this purpose, a biofilm reactor compatible to MRI with an internal RF-coil will be designed. (uni-bremen.de)
The aim of this study was to employ the CDC Biofilm Reactor to evaluate the capacity of F. psychrophilum to form biofilms on stainless steel, polystyrene, polyurethane, and polycarbonate. (pucv.cl)

An established biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)
A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. (cdc.gov)
The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Biofilms are an ensemble of microbial cells irreversibly associated with a surface and enclosed in an essentially self-produced matrix. (intechopen.com)

capitis, Cupriavidus metallidurans and Acinetobacter radioresistens are biofilm forming organisms that have been chosen as model organisms. (sckcen.be)
Bravo and colleagues [ 1 ] investigated the ability of Acinetobacter pittii strains to form biofilms after long-term desiccation if they were fed with nutrient media. (medscape.com)
These findings are consistent with a tendency of other Acinetobacter species to survive on dry surfaces, form biofilms, and contribute to nosocomial outbreaks. (medscape.com)

They require the presence of water, and form on a solid substrate, on soft tissue surfaces within living organisms and at liquid-air interfaces. (thepigsite.com)
They can occur on solid substratum's in contact with moisture, on soft tissue surfaces in living organisms and at liquid-air interfaces. (thepigsite.com)
The BIOFILMS experiment will study the biofilm formation potential of these organisms in microgravity on the International Space Station on inert surfaces (stainless steel AISI 304) as well as antimicrobial active copper (Cu) based metals that have undergone specific surface modification by Ultrashort Pulsed Direct Laser Interference Patterning (USP-DLIP). (sckcen.be)
A. Organisms that live in colonies and form a protective covering over them are called biofilms. (allergyconsumerreview.com)
Steam kills the entire colony and removes the organisms from the surface. (allergyconsumerreview.com)

Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
Two well-studied E. faecalis adhesins, aggregation substance (AS) and endocarditis- and biofilm-associated pili (Ebp), both contribute to biofilm formation on abiotic surfaces and in endocarditis, suggesting that they may be expressed at the same time. (nih.gov)
Aggregating and forming biofilms on biotic or abiotic surfaces are ubiquitous bacterial behaviors under various conditions. (brad.ac.uk)

They found that at low concentrations, the compound, DAPG (the acronym stands for 2,4-diacetylphloroglucinol), produced by the bacterium Pseudomonas protegens, did not kill the experimental target bacterium, Bacillus subtilis, but merely prevented it from forming biofilms. (sciencedaily.com)
5.2 The purpose of this test method is to direct a user in how to grow, treat, sample and analyze a Pseudomonas aeruginosa biofilm using the MBEC Assay. (astm.org)
1.1 This test method specifies the operational parameters required to grow and treat a Pseudomonas aeruginosa biofilm in a high throughput screening assay known as the MBEC (trademarked) 2 (Minimum Biofilm Eradication Concentration) Physiology and Genetics Assay. (astm.org)

If further testing confirms its long-term stability and successful biofilm prevention, coatings based on the liquid-treated surface concept could be applied to a variety of critical components that are known to be susceptible to biofilm fouling, such as water treatment hoses and filters, or to parts that come in close contact with astronauts, such as gloves or food preparation surfaces. (netbalaban.net)
THE RESULTS HAVE LED TO lively discussions about biofilm coatings within the industry. (lu.se)

Attachment is a complex process regulated by diverse characteristics of the growth medium, substratum, and cell surface. (cdc.gov)
and prevention of microbe surface attachment. (wikipedia.org)
They prevent biofilm formation by interfering with the attachment and expansion of immature biofilms. (wikipedia.org)
What Peg Dirckx is illustrating here are the four basic components of biofilm, which is attachment, microcolony formation, quorum sensing to form a mature colony, and then reproduction. (hstalks.com)
It is crucial to understand bacterial gene regulatory networks and decision-making processes for biofilm formation upon initial surface attachment. (brad.ac.uk)
Several hypotheses and studies have suggested that extracellular macromolecules and appendages play important roles in bacterial responses to the surface attachment. (brad.ac.uk)
Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
This is important because past work has found that microgravity can have a significant influence on biofilm morphologies, attachment behavior, and gene expression, according to McBride. (netbalaban.net)

Biofilm is established on the pegs under batch conditions (that is, no flow of nutrients into or out of an individual well) with gentle mixing. (astm.org)
On the other hand, the biofilm limits the diffusive transport of nutrients and reaction products. (uni-bremen.de)
it even provides something like a primitive circulatory system, distributing nutrients to cells throughout the biofilm. (technologyreview.com)
Despite the reduction in culturability over time, all strains that recovered from starvation on solid-surface experiments retained their capacity to form biofilms after rehydration, an addition of nutrients, and changing temperature. (medscape.com)

Researchers have shown that a known antibiotic and antifungal compound produced by a soil microbe can inhibit another species of microbe from forming biofilms--microbial mats that frequently are medically harmful--without killing that microbe. (sciencedaily.com)
Researchers assessed 61 terminally cleaned items from three hospitals in the United Kingdom and used culture-based methods and scanning electron microscopy to study the presence of dry biofilm. (infectioncontroluniversity.com)
April 25, 2016 - Researchers at Ben-Gurion University of the Negev (BGU) have developed an innovative anti-biofilm coating, which has significant anti-adhesive potential for a variety of medical and industrial applications. (eurekalert.org)
Researchers at the unit of food microbiology at Vetmeduni Vienna looked at biofilms in an Austrian meat processing environment that included pork, poultry and beef. (foodsafetynews.com)
Naturally occurring viruses can attack biofilms, but the researchers' genetic modifications increased viruses' effectiveness by two orders of magnitude. (technologyreview.com)
The new technique will allow researchers to build bacteriophage cocktails for attacking all kinds of biofilms. (technologyreview.com)
To the researchers' surprise, the liquid-infused surface performed even better in space than it did on Earth at preventing microbial adhesion. (netbalaban.net)

In addition to the pure science implications of these studies, a potential application of this would be to integrate such chips into medical devices that are common sites of biofilm formation, such as catheters, and then use the chips to limit bacterial colonization. (sciencedaily.com)

Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)

Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down- regulation of specific genes. (cdc.gov)
Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)
Conclusion: Staph aureus incorporated into environmental DSB and covered by extracellular polymeric substances readily contaminates gloved hands and can be transferred to another surface. (infectioncontroluniversity.com)
Biofilms are sessile microbial communities growing on surfaces, frequently embedded in a matrix of extracellular polymeric substances (also known as slime). (medscape.com)
Differences in architecture and cell-surface morphology were observed in biofilms formed by the M1- and M41-wild-type strains, accompanied by varying amounts of deposited extracellular matrix and differences in cell-to-cell junctions within each biofilm. (cdc.gov)

While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)

Our study identified a novel mechanism maintaining Bacillus subtilis and Mycobacterium smegmatis biofilms-active production of calcite minerals. (nature.com)
For the study, first author Matthew Powers, an undergraduate student in Shank's lab, used a strain of B. subtilis--a species commonly used in lab experiments--that fluoresces when genes for biofilm formation are being shut off. (sciencedaily.com)
This study advances our understanding of how E. faecalis , a ubiquitous member of the human gut microbiome and an opportunistic pathogen, uses multiple surface structures to evolve and thrive. (nih.gov)
Therefore, the microbial diversity of biofilms in the different pipe materials and the effect on particle concentration on biofilm formation were investigated in this study. (mdpi.com)
OBJECTIVE: To study the phagocytosis of macrophages on the early biofilm cells of E. coli on polydimethylsiloxane, a biomaterial with different surface hardness. (syr.edu)
In the present study, the antiadhesive properties of titanium surface functionalization based on the "slippery liquid-infused porous surfaces" (SLIPS) principle were demonstrated and the underlying mechanism was analyzed. (nanosurf.com)
In this study, nine healthcare personnel were randomly assigned to assess surfaces 30 seconds and 3 minutes after bleach application with and without Highlight. (medscape.com)
In this study, we demonstrate that surface-associated streptococcal collagen-like protein-1 (Scl1) plays an important role in GAS biofilm formation. (cdc.gov)
Conclusion: Resins modified with silver presented reduction of S. mutans biofilm on their surfaces, according to the conditions of this study. (bvsalud.org)

Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
To assess the possible roles of biomineralization in biofilm development, we grew wild-type B. subtilis cells on media in the presence or absence of calcium acetate as a calcium source. (nature.com)
Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
All examined bath toys revealed notable biofilms on their inner surface, with average total bacterial numbers of 5.5 × 106 cells/cm2 (clean water controls), 9.5 × 106 cells/cm2 (real bath toys), and 7.3 × 107 cells/cm2 (dirty water controls). (discovermagazine.com)
However, staining of residual biofilm showed that live S. aureus cells remained. (infectioncontroluniversity.com)
1.4 This test method describes how to sample the biofilm and quantify viable cells. (astm.org)
The peripheral cells of the biofilm have the advantage of direct access to glutamate. (nature.com)
Cells in the interior, on the other hand, depend on the leftover glutamate that diffuses inwards in the biofilm, but do not lose gaseous molecules as rapidly as the peripheral cells. (nature.com)
Thus cells in different regions of the biofilm will likely exhibit differences in their metabolic behavior 7 . (nature.com)
1) E. coli were inoculated on the surface of three kinds of hard materials to incubate bacterial biofilm cells. (syr.edu)
CFU cell counting, flow cytometry and inverted fluorescence microscope were used to detect the difference in macrophages Phagocytosis of E. coli biofilm cells on surface hardness materials. (syr.edu)
CFU cell count was applied to detect macrophages phagocytosis of E. coli biofilm cells on materials with different surface hardness. (syr.edu)
3) The results show that with the increase of the hardness of polydimethylsiloxane, the efficiency of macrophages phagocytosis of E. coli biofilm cells is higher. (syr.edu)
The external part of the biofilm matrix, a structure lying external to bacterial cells. (planteome.org)
The observed morphotypes were in remarkable agreement with the differential strength of adhesion hypothesis proposing that a tug-of-war among surface structures of different cells governs cell sorting. (elifesciences.org)
Then, the numbers of viable cells recovered from the biofilms were counted through the serial dilution method. (bvsalud.org)
The addition of cAMP to cells treated with α-l-fucopyranoside restored the yeast-hyphae switch and the biofilm level to that of the untreated control. (lu.se)
In the β-d-xylopyranoside treated cells, the biofilm level was only partially restored by the addition of cAMP, and these cells remained mainly as yeast-form cells. (lu.se)

Biofilm growth reactors are engineered to produce biofilms with specific characteristics. (astm.org)

They communicate and colonize with other germs to build a tough, protective biofilm that can withstand even the strongest disinfectants. (infectioncontroluniversity.com)
Conclusion: Current cleaning practices and disinfectants are suboptimal and reinforce that MROs are incorporated into hospital surfaces' film. (infectioncontroluniversity.com)
In fact, biofilms have grown in disinfecting concentrations of quaternary disinfectants. (infectioncontroluniversity.com)
Biofilms are best removed by oxidative disinfectants (Virkon® S or Hyperox® from DuPont Animal Health Solutions). (thepigsite.com)
Brooklyn, New York), which temporarily colorizes chlorine-based disinfectants and fades to undetectable within approximately 5 minutes when applied to surfaces. (medscape.com)
The data showed that addition of Highlight to chlorine-based disinfectants increased the ability of personnel to identify high-touch surfaces with suboptimal application of disinfectant. (medscape.com)

Recently, a minimal mathematical model has been employed to describe the oscillations in Bacillus subtilis biofilms. (nature.com)
In experiments using a microfluidics chamber, oscillations were observed in the growth of Bacillus subtilis 4 which was supplied with glutamate on one end of the chamber while the waste products of the biofilm were washed off at the other end at a constant rate. (nature.com)

S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
In summary, the GAS surface adhesin Scl1 may have an important role in biofilm-associated pathogenicity. (cdc.gov)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
Instead, at higher cell densities during biofilm formation, Ebp and AS differentially contribute to biofilm development and structure, synergizing to promote maximal biofilm formation. (nih.gov)

Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)

Here, we examined two well-characterized adhesins in Enterococcus faecalis , aggregation substance and endocarditis- and biofilm-associated pili, and found that they exhibit distinct functional contributions depending on the growth stage of the bacterial community. (nih.gov)
Regulation of biofilm growth is complex and still largely elusive. (nature.com)
After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. (netbalaban.net)
The results show that textured surfaces impregnated with a lubricant were highly successful at preventing biofilm growth during their long exposure in space. (netbalaban.net)
In both marine and freshwater environments, algae - particularly green algae and diatoms - make up the dominant component of surface growth communities. (wikipedia.org)
Biofilm growth was poor on polyurethane surfaces. (pucv.cl)
To discover methods of reducing the biofilm growth on some materials is challenging both for the science and industry 9 . (bvsalud.org)
The antimicrobial activity of some materials show when nanoparticles are added has been recently demonstrated and largely studied as the new method to allow controlling the microbial biofilm growth on surfaces 8,17,18 . (bvsalud.org)
Thus, innovations enabling the control of biofilm growth on such materials have been the aim of many researches, for example, the use of silver nanoparticles and other alternative compounds 4,13,15,25 . (bvsalud.org)

To avoid the undesirable effects of leaching, antimicrobial agents can be immobilized on device surfaces using long, flexible polymeric chains. (wikipedia.org)
What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin. (bvsalud.org)

In addition, some determinants for biofilm formation were assessed, using six identical bath toys under controlled conditions with either clean water prior to bathing or dirty water after bathing. (discovermagazine.com)
GAS biofilm, which is an in vitro equivalent of tissue microcolony, has only recently been studied and little is known about the specific surface determinants that aid biofilm formation. (cdc.gov)

A motility (motB) mutant of the recipient strain exhibited defects in biofilm formation at the "hot spots" and conjugation, which were recovered by complementing the motB gene on a plasmid. (syr.edu)

Anatomy1

Organisms13

Diseases2

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes13

Disciplines and Occupations1

Technology, Industry, Agriculture6

Health Care4

Bacteria59

Microorganisms21

Reduce biofilm formation3

Staphylococcus4

Streptococcus4

Aureus biofilms3

Pathogens3

Antibiotics7

Adhesion and biofilm formation3

Reduction of biofilm formation2

Abstract2

Characterization5

Infections17

Albicans11

Anti-biofilm agents2

Known as biofilms2

Contamination5

Polysaccharide5

Control of biofilms2

Presence of biofilms1

Disinfection4

Proteins3

Promote biofilm2

Biomaterials4

Mature biofilm2

Relevant biofilm1

Examine biofilms2

Microbes7

Fungal4

Incubation3

Antimicrobial surfaces3

Polysaccharides2

Reactor2

Microbial cells4

Acinetobacter3

Organisms5

Abiotic3

Pseudomonas3

Coatings2

Attachment8

Nutrients4

Researchers7

Catheters1

Inhibits1

Extracellular5

Multi-species1

Study9

Cells18

Reactors1

Disinfectants6

Bacillus2

Pathogenicity2

Differentially2

Inhibition1

Growth9

Polymeric2

Determinants2

Motility1