Subunit recombinant fusion proteins. Medical search. Frequent questions

These subunit vaccines demonstrate extremely good safety against both pneumonic and systemic types of plague [2] in mouse versions. (edrc2013.org)
Among the potential restrictions of the subunit vaccines can be that F1 is not needed for complete virulence of em Con. (edrc2013.org)
As the recombinant subunit vaccines are amazing in experimental pets and offer safety against F1 minus strains of em Y. pestis /em [2], the addition of additional antigens using the LcrV/F1 subunit vaccine applicants could enhance the ability from the ensuing vaccine to provide safety against multiple em Y. pestis /em strains, or the brand new antigens could possibly be created as distinct vaccine applicants. (edrc2013.org)
There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein which plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. (sydlabs.com)
The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. (ac.ir)
These systems can function as inexpensive platforms for the large scale production of recombinant pharmaceuticals or subunit vaccines. (waw.pl)
Plant-produced recombinant subunit vaccines and plant-made VLPs were successfully tested in clinical trials (Phase I and II) that confirmed their tolerance and immunogenicity. (waw.pl)
The choice of an expression system is essential when developing subunit vaccines, as it ensures the effective synthesis of the correctly folded target protein, and maintains its antigenic and immunogenic properties. (pimac.org)
These fusion proteins are the basis of many subunit vaccines, a type in which a part of the pathogen being vaccinated against is injected to prompt the body to efficiently raise an immune response that can protect against the live virus. (nature.com)
Little is known of the T cell repertoire these recombinant vaccines induce. (biomedcentral.com)
T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (bvsalud.org)

Appropriately, the TTSS needle complicated proteins, YscF, was chosen to research its potential like a protecting antigen. (edrc2013.org)
Summary With this scholarly research we looked into YscF, a surface-expressed proteins from the em Yersinia pestis /em type III secretion organic, like a protective antigen against experimental plague disease. (edrc2013.org)
Current attempts for vaccine advancement have centered on two proteins: LcrV (also called the V antigen) as well as the capsular F1 antigen [2]. (edrc2013.org)
The results of these ELISAs using truncated recombinant VP2 and VP3 subunit proteins as coating antigen showed that VP2-345N, VP2-396N and VP3-246M gave good immunoreactivity with CAV-positive chicken sera compared to the other subunit proteins. (biomedcentral.com)
Hemagglutinin (HA) is a major surface antigen of the influenza virus, thus it is in the centre of interests of various subunit vaccine engineering programs. (waw.pl)
Therefore, by synthesizing the N gene sequence of SARS-CoV-2, constructing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in E.coli and obtained 15 mAbs against SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established. (pimac.org)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
The specificity of FSC231 for the PICK1 PDZ domain was supported by the lack of binding to PDZ domains of postsynaptic density protein 95 (PSD-95) and glutamate receptor interacting protein 1 (GRIP1). (ku.dk)
Answer: SARS-CoV-2 S1 subunit-based immunoassay kits have better specificity though lower sensitivity. (sydlabs.com)
Compartmental specificity is encoded to a remarkable degree in the functional partnering of SNARE proteins, a fact which is in no way inconsistent with the emerging contribution of upstream regulatory components (like rabGTPases and tethering complexes) to domain/compartment specificity. (yale.edu)
In this study, we obtained 14 high-titer and high-specificity monoclonal antibodies, and the test strips exclusively react with the SARS-CoV-2-N protein and no cross-reactivity with other coronavirus and also recognize the recombinant N protein of Delta (B.1.617.2) variant. (pimac.org)

TrSOD, TAT-TrSOD-Yfp (fused to yellow fluorescent protein) and Ab-TrSOD were expressed in E.coli and purified as soluble proteins. (pimac.org)
The inner fluid can contain cargo molecules such as nucleic acids or soluble proteins. (biomedcentral.com)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

The defined antigenicity potential of these chimeric subunit proteins produced by expression in E. coli seem to have potential and could be useful in the future for the development of the CAV diagnostic tests based on a subunit protein ELISA system. (biomedcentral.com)
Various forms of recombinant HA, including monomers, trimers, virus like particles (VLPs) or chimeric proteins containing its fusion with other polypeptides were obtained and shown to maintain a proper antigenicity. (waw.pl)
In the present study, a recombinant chimeric protein rGB contriving immunologically active regions of Gapdh and bvrS from B. abortus S19 was constructed, cloned and expressed in E. coli host cell. (journalcra.com)

In addition, the culture was immunofluorescently labeled with Cy2 conjugated to antibodies that target peroxisomal membrane protein 70 ( PMP 70 ), an abundant and integral membrane component of peroxisomes. (fsu.edu)
Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
We emphasized product quality aspects regarding the fusion protein itself and in addition, post-translational characterization of the subunits in comparison to human antibodies and rhEpo. (boku.ac.at)
Interacting proteins were resolved by conventional NuPAGE and analyzed by Western blotting using anti-aldolase-A/B (D-18 1 anti-GAPDH (V-18 1 anti-PGK (E-20 1 anti-PFK (E-16 1 and anti-enolase antibodies (H-300 1 To study direct interactions of ARNO with either V-ATPase a-isoforms or aldolase-B we performed pull-down experiments with recombinant proteins. (crispr-reagents.com)
Recombinant SARS-CoV-2 spike S1-Fc protein , biotinylated His-tag and Fc-fused spike S1 subunits , SARS-CoV-2 spike S1 antibodies, immunoassay kits, custom antibody and kit development services are available. (sydlabs.com)
Answer: SARS-CoV-2 S1 subunit is better since it is bigger with more epitopes possibly better for antibody development, especially for neutralizing antibodies. (sydlabs.com)
The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L12 antibodies. (ac.ir)
That study showed that in naturally infected individuals the majority of potent neutralizing antibodies were those responding to the pre-fusion form of the protein, not the post-fusion form. (nature.com)
These findings demonstrate the potential efficacy of fusion protein rGB as a candidate subunit vaccine and showed the development of memory response and functional role of antibodies in protection against Brucella infections. (journalcra.com)

The SARS-CoV-2 spike or fusion protein is a trimer: a group of three units, each consisting of a receptor-binding subunit, S1, and a subunit responsible for fusion, S2. (nature.com)

We previously identified a lipopeptide, EK1C4, by linking cholesterol to EK1, a pan-CoV fusion inhibitory peptide via a polyethylene glycol (PEG) linker, which showed potent pan-CoV fusion inhibitory activity. (bvsalud.org)
In the process of membrane fusion, envelope protein exposes fusion peptide, followed by an insertion into the cell membrane or endosomal membrane. (bvsalud.org)
Polyclonal antisera raised against a 28-residue peptide (corresponding to part of the disintegrin domain) and two recombinant fusion proteins identified a 90 kDa protein in testicular sperm extracts and a 60 kDa protein in caput and cauda epididymidal sperm extracts, the predicted sizes for rat fertilin β precursor and mature protein respectively. (edu.au)
Ab-TrSOD, when compared to TrSOD, promoted a decrease in the formation of ROS of 19 and 14% at the concentrations of 1 and 6 μmol/L, respectively, indicating that this joplinks Recombinant Human Regulator of G-protein form was more effective in reducing oxidative stress compared to SOD without the cell penetrating peptide (CPP). (pimac.org)

The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. (creativebiomart.net)

Recombinant 2019-nCoV Spike RBD(G446S) Protein (YP_009724390.1) (Arg319-Phe541(G446S)) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus. (creativebiomart.net)
The recombinant 2019-nCoV Spike RBD(G446S) Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. (creativebiomart.net)

2006): Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells. (boku.ac.at)
By using these techniques for the characterization of the recombinant human Epo-Fc (rhEpo-Fc) molecule itself and furthermore, for the separate characterization of both subunits, we could clearly show that no significant differences in the core glycan structures compared to rhEpo and human antibody N-glycans were found. (boku.ac.at)
The expression, production and antigenic characterization of CAV viral proteins such as VP1, VP2 and VP3, and their use in the development of diagnostic kit would be useful for CAV infection prevention. (biomedcentral.com)
To carry out the further antigenic characterization of the immunorelevant domains of the VP2 and VP3 proteins, five recombinant VP2 subunit proteins (VP2-435N, VP2-396N, VP2-345N, VP2-171C and VP2-318C) and three recombinant VP3 subunit proteins (VP3-123N, VP3-246M, VP3-366C), spanning the defined regions of VP2 and VP3 were separately produced by an E. coli expression system. (biomedcentral.com)

In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. (bio-techne.com)
This work aimed to clone, express, purify and evaluate the protective effect antioxidant of this enzyme on skin cells when fused to transactivator of transcription (TAT) protein transduction domain of HIV-1 and Abalone (Ab) peptides to allow cell penetration. (pimac.org)

The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. (biolegend.com)

SARS-CoV-2 B.1.1.7 Spike Protein S1, amino acid Val16-Arg685 (Accession # QHD43416.1), with (HV69-70del, Y144del, N501Y, A570D, P681H) and a C-terminal 8-His tag was expressed in CHO cells. (biolegend.com)
The 678 amino acid recombinant protein has a predicted molecular mass of approximately 76 kD. (biolegend.com)
the primary amino acid sequences of the proteins coded by marburg and ebola-zaire filoviruses were studied by computer programs to search for putative proteolytic cleavages which yield nonapeptides with motifs of binding to known hla class i haplotypes. (liverpool.ac.uk)

However, overproduction of recombinant proteins in mammalian cells is well established, analysis of product quality of complex products for different purposes, such as product specification, purification issues, batch to batch consistency and therapeutical consequences, is required. (boku.ac.at)
Source: recombinant SARS-CoV-2 spike protein S1 subunit (Spike S1 protein, Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus. (sydlabs.com)

were prepared by constructing one novel subunit vaccine by mixing or fusing the three proteins and combining them with aluminum hydroxide adjuvant, and the immunogenicity and protective properties of the vaccine was evaluated in mice. (bvsalud.org)
An analysis of the sera from mice immunized with both variants of the protein revealed that the mRBD expressed in CHO cells provides a significantly stronger humoral immune response compared with the RBD expressed in E.coli cells. (pimac.org)
Group of mice immunized with purified rGB protein elicited both Th1 and Th2 mediated immunity as indicated by the higher secretion of IgG2a and IgG1 antibody isotypes in the sera. (journalcra.com)

Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein Binding Activity Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10565-CV) binds Recombinant Human ACE-2 His-tag ( 933-ZN ) in a functional ELISA. (bio-techne.com)
Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant SARS-CoV-2 Spike RBD His-tag Protein (10565-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa and 108-120 kDa, respectively. (bio-techne.com)

A particle according to any one of the preceding claims, wherein heterologous polypeptide sterically hinders fusion of an enveloped viral particle with an eukaryotic cell to which it is bound. (sumobrain.com)
This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. (biomedcentral.com)
The possibility to locate heterologous proteins into the P9-lipid vesicles facilitates the production of vesicular structures with novel properties. (biomedcentral.com)

The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. (bdbiosciences.com)
This recombinant human monoclonal antibody (VF0035) is specific for SARS-CoV S protein recognition. (creative-biolabs.com)

abstract = 'Proteins containing PSD-95/Discs-large/ZO-1 homology (PDZ) domains play key roles in the assembly and regulation of cellular signaling pathways and represent putative targets for new pharmacotherapeutics. (ku.dk)
abstract = "Fertilin α and β are members of the MDC (metalloproteinase-like, disintegrin-like, cysteine-rich) protein family and are expressed on the sperm surface where they have been proposed to play a role in mammalian fertilization. (edu.au)

S2 contains basic elements needed for the membrane fusion. (creativebiomart.net)
S2 provides essential elements needed for membrane fusion. (biossusa.com)
Type â enveloped viruses bind to cell receptors through surface glycoproteins to initiate infection or undergo receptor-mediated endocytosis and initiate membrane fusion in the acidic environment of endocytic compartments, releasing genetic material into the cell. (bvsalud.org)
SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. (bio-techne.com)
In subsequent years we found that we had reconstituted vesicle trafficking in the Golgi, including the process of membrane fusion. (yale.edu)
S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. (creative-biolabs.com)
This includes the S1 N-terminal domain (NTD) or the receptor-binding domain (RBD) and the S2 fusion domains that mediate viral-membrane fusion following virus-host cell attachment at the angiotensin-converting enzyme 2 (ACE2) receptor. (news-medical.net)

The Fc domain of IgG contributes several important properties to the fusion protein, including increased half-life in the bloodstream, enhanced binding to Fc receptors on immune cells, and the ability to activate complement. (wikipedia.org)
The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. (biolegend.com)
For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)

The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. (creativebiomart.net)

Contrary to expectations, recombinant fusion of the subunits have not stabilized PP7 virus-like particles against thermal denaturation, whilst disulphide bonds between coat protein dimers greatly increased the viral particles' stability. (biomedcentral.com)

It can be concluded that ECP001 is a novel effective multicomponent subunit vaccine candidate with potential as BCG Initial Immunisation-ECP001 Booster Immunisation or therapeutic vaccine for M. tuberculosis infection. (bvsalud.org)

Answer: Different from the SARS-CoV-2 spike protein in the virus, the recombinant SARS-CoV-2 spike S1 subunit is a monomer. (sydlabs.com)
Question: Does Syd Labs have SARS-CoV-2 spike protein S1 subunit-based rapid test kit or ELISA kit? (sydlabs.com)

Escherichia coli, as the host of Mtb, was used for cloning and protein purification. (bvsalud.org)
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. (elsevierpure.com)

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN ). (bio-techne.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
Dose response curve for the biotinylated recombinant human ACE2-His fusion protein with the EC50 of 0.2 ug/ml determined by ELISA. (sydlabs.com)
Answer: Yes, please check our SARS-CoV-2 IgG ELISA kit (S protein). (sydlabs.com)
The purified recombinant CAV VP1, VP2 and VP3 proteins were then used as antigens in order to evaluate their reactivity against chicken sera using indirect ELISA. (biomedcentral.com)
Purified recombinant VP2-396N and VP2-345N subunit proteins, which span defined regions of VP2, were demonstrated to have good antigenicity and higher sensitivities than VP3-246M and were able to recognize CAV-positive chicken serum using an ELISA assay. (biomedcentral.com)

Within the cytoplasm, lysophosphatidylcholine ( lyso-PC ) induces AP-1 activity and c-jun N-terminal kinase activity ( JNK1 ) by a protein kinase C-independent pathway. (fsu.edu)
We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. (rockland.com)
Here we describe the first small-molecule inhibitor (FSC231) of the PDZ domain in protein interacting with C kinase 1 (PICK1) identified by a screening of approximately 44,000 compounds in a fluorescent polarization assay. (ku.dk)

The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition. (biolegend.com)
Further conformational changes ensue in which the type 1 envelope protein forms a typical six-helix bundle structure, shortening the distance between viral and cell membranes so that fusion can occur. (bvsalud.org)
The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. (sydlabs.com)
Recent work published in The EMBO Journal and EMBO Reports reveals a novel role for the protein TECPR1 as a sensor for stressed membranes and regulator of lysosomal membrane repair. (babraham.ac.uk)
In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. (babraham.ac.uk)
We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes. (babraham.ac.uk)

The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
shown in red), a poorly characterized protein implicated in oxidative stress response. (upstate.edu)
The potential mechanisms probably involved interfering Rac1 geranylgeranylation, inhibiting the assembly of NADPH oxidase cytosolic regulatory subunits, reducing oxidative injury, and decreasing ERK1/2 and JNK phosphorylation. (hindawi.com)

We assessed replication-restricted single-injection recombinant vesicular stomatitis vaccine NiV vaccine vectors expressing the NiV glycoproteins against NiV B challenge in African green monkeys. (cdc.gov)
A set of viral vectors was thus generated, all of which contained SARS-CoV-2 coding sequences for the spike S1 subunit, the NTD, the RBD, extended RBD, and the S2 core region that contains its fusion domain. (news-medical.net)

The three S1 and S2 subunits form a fusion spike embedded in the viral membrane and held in a very particular conformation until it binds to a target cell. (nature.com)

The protein is a fusion of the extracellular domain of the CD4 receptor and the Fc domain of human immunoglobulin G (IgG), the most abundant antibody isotype in the human body. (wikipedia.org)
When recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1 is immobilized at 2 μg/mL, recombinant human ACE2-Fc Chimera (Cat. (biolegend.com)
The samples were tested in a binding assay with recombinant human ACE2-Fc Chimera. (biolegend.com)
In this study, we found that EKL1C also exhibited broad-spectrum fusion inhibitory activity against human immunodeficiency virus type 1 (HIV-1) infection by interacting with the N-terminal heptad repeat 1 (HR1) of viral gp41 to block six-helix bundle (6-HB) formation. (bvsalud.org)
In this context, we have expressed a homodimer fusion protein in CHO cells which consists of two identical polypeptide chains, in which our target protein, recombinant human erythropoietin (rhEpo), is N-terminally linked with the Fc part of a human IgG(1) molecule. (boku.ac.at)
Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. (rockland.com)

The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). (bio-techne.com)
Question: Is Syd Labs's recombinant SARS-CoV-2 spike S1 protein trimer or monomer? (sydlabs.com)

Three CAV viral proteins VP1, VP2 and VP3 was separately cloned and expressed in recombinant E. coli . (biomedcentral.com)
Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)
Recombinant HCV Fusion protein produced in E. coli containing HCV core (120 a.a. (creativebiomart.net)
The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)

The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)
Binding to a receptor triggers a very rapid structural change, with some parts of the protein flipping through 180°, pulling the viral membrane and the host membrane close together to allow them to fuse. (nature.com)

Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (creativebiomart.net)

In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the L7/L12 ribosomal gene was amplified by PCR. (ac.ir)
The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. (ac.ir)

Our lab is working to elucidate the underlying mechanisms of vesicular transport within cells and the secretion of proteins and neurotransmitters. (yale.edu)

For example, the fusion protein has been shown to induce the production of cytokines, such as interleukin-2 (IL-2) and interferon-gamma (IFN-γ), which are important for the activation and proliferation of immune cells. (wikipedia.org)

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). (bio-techne.com)
The phi6 major envelope protein P9 and the non-structural protein P12 are essential for the envelopment of its virions. (biomedcentral.com)
Early studies on nonsense mutants of phage phi6 suggested that the major envelope protein P9 and the non-structural protein P12 are the only proteins needed for phi6 virion envelopment [ 23 ]. (biomedcentral.com)
Further structural modification was needed to achieve stability, whereas fusion proteins clamped using the gp41 fragments were stable on their own. (nature.com)
These encoded sequences for the non-structural protein NSP3 and some parts of the viral spike. (news-medical.net)

YscF can be a surface Gadoxetate Disodium area localized proteins that's needed is both Gadoxetate Disodium to secrete Yops also to translocate poisons into eukaryotic cells [16-19]. (edrc2013.org)

Autophagy is a fundamental catabolic process coordinated by a network of autophagy-related (ATG) proteins. (babraham.ac.uk)
These ATG proteins also perform an important parallel role in 'noncanonical' autophagy, a lysosome-associated signaling pathway with key functions in immunity, inflammation, cancer, and neurodegeneration. (babraham.ac.uk)
Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. (babraham.ac.uk)

Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. (elsevierpure.com)
These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)
Jensen-Shannon and Grantham scores were computed for mutations in protein-coding genes to evaluate whether they affected protein function. (cdc.gov)

Entry inhibitors targeting viral envelope proteins, or host factors, are effective antiviral agents and have been widely studied. (bvsalud.org)
The only bacteriophages known to have a lipid envelope around their protein capsids are the members of the Cystoviridae family [ 6 ]. (biomedcentral.com)

Immunization strategies against this morbillivirus using recombinant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit differentiation of infected from vaccinated animals. (biomedcentral.com)

Homer proteins are components of postsynaptic density (PSD) and play a crucial role in coupling diverse target molecules. (jneurosci.org)

The kinetics of CS1 and MDH1 activities of the fusion protein were compared to those of the free enzymes. (lu.se)
It was observed that AAT was a less effective competitor for OAA when the CS1/MDH1 fusion protein is used than when the separate enzymes are employed. (lu.se)
In addition, the transient time for the coupled reaction sequence was less for the fusion protein than for the free enzymes. (lu.se)
The 3T3 cells were exposed to the oxidant agent tert-butyl hydroperoxide (tBH) and evaluated for ROS generation, in the presence or not of the recombinant enzymes. (pimac.org)
Geranylgeranyl transferase-I (GGTase-I) is one of the key enzymes mediating protein isoprenylation during the posttranslational modification, especially the geranylgeranylation of small GTPase, Rac1 [ 6 ]. (hindawi.com)

Using reverse genetics , recombinant rotaviruses of species A have been created. (news-medical.net)
Earlier, this was shown to be susceptible to modification using reverse genetics, whereby two separate proteins could be expressed by this open reading frame (ORF). (news-medical.net)

The same conformational change occurs when the spike protein is isolated or made by recombinant technology to produce a soluble subunit vaccine. (nature.com)

The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)
Flavinylation of Fp in B. subtilis does not require the Ip subunit or the membrane anchor but depends on folding of the Fp polypeptide into a state that can bind FAD and subsequent mediate covalent binding of the FAD [3, 4]. (lu.se)

CD4 immunoadhesin is a bifunctional protein that has the ability to block HIV infection, inhibit autoreactive T-cell activation, and potentially modulate immune responses. (wikipedia.org)
These results suggest that HR1 is a common target for the development of broad-spectrum viral fusion inhibitors and EKL1C has potential clinical application as a candidate therapeutic or preventive agent against infection by coronavirus, HIV-1, and possibly other class I enveloped viruses. (bvsalud.org)
Up to the present, systematically assessing viral protein antigenicity and/or determining the immunorelevant domain(s) of viral proteins during serological testing for CAV infection has never been performed. (biomedcentral.com)

This review focuses on entry inhibitors that target the six-helical bundle core against highly pathogenic enveloped viruses with class I fusion proteins, including retroviruses, coronaviruses, influenza A viruses, paramyxoviruses, and filoviruses. (bvsalud.org)

Many of the protein necessary for the building or function of the sort III secretion program (TTSS) complicated could possibly be ideal contenders to meet up the desired top features of a vaccine applicant. (edrc2013.org)
This is actually the first are accountable to our understanding employing a conserved proteins from the sort III secretion complicated of the gram-negative pathogen as an applicant for vaccine advancement. (edrc2013.org)

Enveloped viruses such as SARS-CoV-2, the virus responsible for COVID-19, infect a host via proteins protruding from their surface that bind to and fuse with a target cell's membrane, allowing the virus to enter a cell. (nature.com)
Previous work had identified three main areas of the trimeric spike proteins of many enveloped viruses like SARS-CoV-2 where modification might improve stabilization and expression. (nature.com)
The resulting rSA11/NSP3-CoV2/S viruses contain 12% more material than rSA11/wt (wildtype recombinant rotavirus). (news-medical.net)
The rSA11/NSP3-CoV2/S viruses produced smaller plaques and lower viral titers in cell culture than the wildtype, perhaps because the RNA elongation time required for transcription of the segment 7 dsRNAs is increased during the replication of the virus, or the longer translation time to synthesize proteins encoded from these RNAs. (news-medical.net)

This is the first report to systematically assess the antigenic characteristics of CAV viral proteins for sero-diagnosis purposes. (biomedcentral.com)
computer simulations of proteolysis of marburg and ebola-zaire filovirus coded proteins to generate nonapeptides with motifs of known hla class i haplotypes and detection of antigenic domains in the viral glycoproteins. (liverpool.ac.uk)
Many of the important antigenic sites on the spike protein become hidden or disappear because they literally change shape. (nature.com)

Stability Testing for Recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1. (biolegend.com)

Question: What is the advantage of SARS-CoV-2 spike S1 protein-based immunoassay kits to nucleocapsid-based kits? (sydlabs.com)
The nucleocapsid (N) protein of Severe Acute Respiratory syndrome Coronavirus 2 (SARS-CoV-2) is involved in viral replication, assembly and immune regulation and plays an important role in the viral life cycle. (pimac.org)

TP53RK belongs to a small subfamily of atypical protein kinases. (rockland.com)

Anatomy12

Organisms12

Diseases1

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes55

Information Science3

Health Care1

Vaccines13

Antigen6

Specificity5

Soluble proteins3

Chimeric3

Antibodies9

Receptor-binding1

Peptide4

Grouping of heterologous1

2019-nCoV2

Characterization4

Peptides2

Contains a receptor bindin1

Amino acid3

Mammalian cells2

Mice3

Spike RBD2

Heterologous4

Monoclonal2

Abstract2

Membrane fusion7

Receptors3

Neutralizing-antibody1

Virus like particles1

Vaccine candidate1

SARS-CoV-2 Spike Protein2

Escherichia2

ELISA6

Kinase5

Membranes6

Oxidative3

Vectors2

Binds1

Human6

Monomer2

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Viral membrane2

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Induce1

Structural5

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Autophagy3

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Enzymes5

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Conformational1

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Viruses4

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Nucleocapsid2

Kinases1