• enzyme
  • All substrates were dephosphorylated by AtZDP, assuming that this enzyme could potentially be involved in double-strand DNA repair. (diva-portal.org)
  • 1999). These reactions are catalysed by members of the sulfotransferase (SULT) enzyme superfamily that transfer a sulfuryl moiety from the co-substrate 3′-phopshoadenosine 5′-phosphosulfate (PAPS). (springer.com)
  • Enzyme activity and substrate specificity of the major cinnamyl alcohol dehydrogenases in sorghum. (plantphysiol.org)
  • The result is a polymerase that is kinetically indistinguishable from the wild-type enzyme, but provides a signal to monitor changes in enzyme structure that result from conformational changes induced by substrate binding. (utexas.edu)
  • This assay provides a quick and efficient method to screen the substrate specificity of para -phenol oxidases, facilitating the enzyme engineering of known para- phenol oxidases and the evaluation of the substrate specificity of novel para -phenol oxidases. (mdpi.com)
  • It catalyses the condensation of tryptamine with secologanin to form strictosidine: tryptamine + secologanin ⇌ {\displaystyle \rightleftharpoons } 3-alpha(S)-strictosidine + H2O Thus, the two substrates of this enzyme are tryptamine and secologanin, whereas its two products are 3-alpha(S)-strictosidine and H2O. (wikipedia.org)
  • In enzymology, a tryptophan alpha,beta-oxidase (EC 1.3.3.10) is an enzyme that catalyzes the chemical reaction L-tryptophan + O2 ⇌ {\displaystyle \rightleftharpoons } alpha,beta-didehydrotryptophan + H2O2 Thus, the two substrates of this enzyme are L-tryptophan and O2, whereas its two products are alpha,beta-didehydrotryptophan and H2O2. (wikipedia.org)
  • In enzymology, a haloalkane dehalogenase (EC 3.8.1.5) is an enzyme that catalyzes the chemical reaction 1-haloalkane + H2O ⇌ {\displaystyle \rightleftharpoons } a primary alcohol + halide Thus, the two substrates of this enzyme are 1-haloalkane and H2O, whereas its two products are primary alcohol and halide. (wikipedia.org)
  • In enzymology, a trypanothione-disulfide reductase (EC 1.8.1.12) is an enzyme that catalyzes the chemical reaction trypanothione + NADP+ ⇌ {\displaystyle \rightleftharpoons } trypanothione disulfide + NADPH + H+ Thus, the two substrates of this enzyme are trypanothione and NADP+, whereas its 3 products are trypanothione disulfide, NADPH, and H+. (wikipedia.org)
  • In enzymology, a riboflavin kinase (EC 2.7.1.26) is an enzyme that catalyzes the chemical reaction ATP + riboflavin ⇌ {\displaystyle \rightleftharpoons } ADP + FMN Thus, the two substrates of this enzyme are ATP and riboflavin, whereas its two products are ADP and FMN. (wikipedia.org)
  • In enzymology, an estradiol 17beta-dehydrogenase (EC 1.1.1.62) is an enzyme that catalyzes the chemical reaction estradiol-17beta + NAD(P)+ ⇌ {\displaystyle \rightleftharpoons } estrone + NAD(P)H + H+ The 3 substrates of this enzyme are estradiol-17beta, NAD+, and NADP+, whereas its 4 products are estrone, NADH, NADPH, and H+. (wikipedia.org)
  • They catalyse the oxidation of primary amines to aldehydes, with the subsequent release of ammonia and hydrogen peroxide, which requires one copper ion per subunit and topaquinone as cofactor: RCH2NH2 + H2O + O2 ⇌ {\displaystyle \rightleftharpoons } RCHO + NH3 + H2O2 The 3 substrates of this enzyme are primary amines (RCH2NH2), H2O, and O2, whereas its 3 products are RCHO, NH3, and H2O2. (wikipedia.org)
  • In prokaryotes, the enzyme enables various amine substrates to be used as sources of carbon and nitrogen. (wikipedia.org)
  • kinetics
  • Additionally, we have characterized the kinetics governing the specificity and discrimination of several widely administered Nucleotide Reverse Transcriptase Inhibitors (NRTI's) used to combat HIV infection including 3TC (Lamivudine), FTC (Emtricitabine), and AZT (Zidovudine) for the wild-type polymerase and mutants with clinical resistance to these compounds. (utexas.edu)
  • cleavage
  • Other endoglycosidases, similar to PNGase F, include endoglycosidase F1, endoglycosidase F2, endoglycosidase F3, and endoglycosidase H. These endoglycosidases have more specificity in cleavage and are less sensitive to protein conformation than PNGase F. All of these endoglycosidases, including PNGase F, can be purified from an almond emulsion or flavobacterium meningosepticum. (wikipedia.org)
  • differences
  • Furthermore, differences in fatty acid chain length specificity of PPT1 and -2, also reported here, are explained by the structure and may provide a biochemical basis for their non-redundant roles. (rcsb.org)
  • alterations
  • Screening of a small library of VAO and EUGO active-site variants for alterations in their substrate specificity led to the identification of a VAO variant (T457Q) with increased activity towards vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol) and a EUGO variant (V436I) with increased activity towards chavicol (4-allylphenol) and 4-cyclopentylphenol. (mdpi.com)
  • proteins
  • 1990) "Identification of Major Nucleolar Proteins As Candidate Mitotic Substrates of cdc2 Kinase" Cell 60:791-801. (patentgenius.com)
  • The Walker A motif is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. (wikipedia.org)
  • kinases
  • Dependent on the initial staurosporine scaffold the organoruthenium complexes have provided marked specificity for the GSK3 and PIM kinases by the introduction of the metallic centre coordinated by a cyclopentadienyl ring and a CO ligand. (wallinside.com)
  • effects
  • Since both phosphorylation and O-GlcNAcylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. (wikipedia.org)
  • activity
  • Substrate preferences at P5 to P3 positions were important in enhancing the main protease activity. (hkmj.org)
  • Super-reactive' substrate sequences were engineered, with more than a 2-fold increase in activity, by combining the best residue choices at P5 to P3 positions. (hkmj.org)